The Vo sector of the vacuolar H+-ATPase is a multi-subunit complex that forms a proteolipid pore. The largest subunit in this complex is the a subunit which has four isoforms (a1-a4). The isoform(s) critical for secretory vesicle acidification has yet to be identified. Using a cell line derived from rat pheochromocytoma in which Voa1 and/or Voa2 had been down-regulated this study revealed that Voa1, and to a lesser extent, Voa2 are critical for acidifying dense-core vesicles (DCVs). The acidification defects resulting from down-regulation of Voa1 and Voa1/ Voa2 were suppressed by the expression of knockdown-resistant Voa1. Defects in DCV acidification resulted in reductions in their transmitter uptake and storage. Lastly, Ca2+-dependent peptide secretion appeared normal in Voa1 and Voa1/ Voa2 knockdown cells. . This study demonstrated that Voa1 and Voa2 cooperatively regulate dense-core vesicle acidification as well as transmitter uptake/storage, while they may not be critical for dense-core vesicle exocytosis.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Document formatted into pages; contains xv, 128 p.; also includes graphics (some col.). Includes bibliographical references (p. 125-128). Available online via OhioLINK's ETD Center

abstract: RF transmitter manufacturers go to great extremes and expense to ensure that their product meets the RF output power requirements for which they are designed. Therefore, there is an urgent need for in-field monitoring of output power and gain to bring down the costs of RF transceiver testing and ensure product reliability. Built-in self-test (BIST) techniques can perform such monitoring without the requirement for expensive RF test equipment. In most BIST techniques, on-chip resources, such as peak detectors, power detectors, or envelope detectors are used along with frequency down conversion to analyze the output of the design under test (DUT). However, this conversion circuitry is subject to similar process, voltage, and temperature (PVT) variations as the DUT and affects the measurement accuracy. So, it is important to monitor BIST performance over time, voltage and temperature, such that accurate in-field measurements can be performed. In this research, a multistep BIST solution using only baseband signals for test analysis is presented. An on-chip signal generation circuit, which is robust with respect to time, supply voltage, and temperature variations is used for self-calibration of the BIST system before the DUT measurement. Using mathematical modelling, an analytical expression for the output signal is derived first and then test signals are devised to extract the output power of the DUT. By utilizing a standard 180nm IBM7RF CMOS process, a 2.4GHz low power RF IC incorporated with the proposed BIST circuitry and on-chip test signal source is designed and fabricated. Experimental results are presented, which show this BIST method can monitor the DUT’s output power with +/- 0.35dB accuracy over a 20dB power dynamic range. / Dissertation/Thesis / Masters Thesis Electrical Engineering 2015

In this research, an innovative phased array antenna module is proposed to implement a high-power, high-efficient and compact C-band radio transmitter. The module configuration, which can be integrated into front-end circuits, was designed as planar layers stacked up together to form a metallic cube. The layers were fabricated by using a Computer Numerical Control (CNC) milling machine and screwed together. The antenna parts and the amplifier units were designed at two opposite sides of the cube to spread the dissipated heat produced by the amplifiers and act as a heat sink. Merging the antenna parts with the amplifier circuits offers additional advantages such as decreasing the total power loss, mass, and volume of the transmitter modules by removing the extra power divider and combiner networks and connectors between them as well as reducing the total signal path. To achieve both a maximum possible radiation efficiency and high directivity, the aperture waveguide antenna was selected as the array element. Four antenna elements have been located in a cavity to be excited equally and the cavity is excited through a slot on its underside so a compact subarray is formed. Antenna measurements demonstrated a 15.5 dBi gain and 20 dB return loss at 10 % fractional bandwidth centered around 5.8 GHz and with more than 98% radiation efficiency. The total dimensions of the subarray are approximately 8*12*4 cm3. The outcoming signal from the amplifiers is transferred into the slot exciting the subarray through a microstrip-to-waveguide transition (MWT). A novel and robust MWT structure was designed for the presented application. The MWT was also integrated with a microstrip coupler to monitor the power from the amplifier output. The measured insertion loss of the MWT along with the microstrip coupler was less than 0.25 dB along with more than 20 dB return loss within the same bandwidth of the subarray. The microstrip coupler shows 38 dB of coupling and more than 48 dB of isolation with negligible effects on the amplifier output signal and the insertion/return loss of the MWT. The amplifier subcomponents consist of power combiners/dividers (PCDs), high power amplifiers (HPAs) and bias circuitry. A Monolithic Microwave Integrated Circuit (MMIC) three-stage HPA was designed in a commercially available 0.15 um AlGaN/GaN HEMT technology provided by National Research Council Canada (NRC) and occupies an area of 4.7*3.7 mm2. To stabilize the HPA, a novel inductive degeneration technique was successfully used. To the best of the author’s knowledge, this is the first time this technique has been used to stabilize HPAs. Careful considerations on input/output impedances of all HEMTs were taken into account to prevent parametric oscillations. Other instability sources, i.e. odd-mode, even-mode, and low frequency (bias circuit) oscillations were also prevented by designing the required stabilization circuits. The electromagnetic simulation of the HPA shows 35 W (45.5 dBm) of saturated output power, 26 dB large signal gain and 29% power added efficiency within the same operating bandwidth as the subarray. The output distortion is less than 27 dB, indicating that the HPA is highly linear. The PCD was designed by utilizing a novel, enhanced configuration of a Gysel structure implemented on Rogers RT-Duroid5880. The insertion loss of the Gysel is less than 0.2 dB while return loss and isolation are greater than 20 dB over the entire bandwidth. The same subarray area (8*12 cm2) has been used for the amplifier circuits and up to eight HPAs can be included in each module. All the above parts of the transmitter module were fabricated and measured, except the MMIC-HPA.

At motor nerve terminals, Hg2+ causes (a) irreversible depolarization, (b) increase in transmitter release, and (c) subsequent irreversible block of transmitter release. All effects are antagonized when a Na channel blocker (tetrodotoxin, TTX) and a Ca channel blocker (Co2+) are present, but not when either blocker is used alone. The effects are not antagonized by TTX plus Co2+ when the mercurial is lipid-soluble (methylmercury). This indicates that the neurotoxic action of Hg2+ is at an intracellular site and that entry is gained through both Na and Ca channels. The results suggest that metals may inhibit transmitter release at either the Ca channel or at the release site, but that irreversible toxicity is due to an intracellular action, possibly involving SH groups.

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The IEEE 802.11 wireless local area network (WLAN) standard supports multiple transmission modes. However, the higher mandatory data rate mode does not necessarily yield higher throughput. This research started from the relationship between the link throughput and the channel's carrier-to-noise (C/N) ratio. Two algorithms are proposed, a throughput optimization and transmitter power saving (TOTPS) algorithm and an extended throughput optimization and transmitter power saving (ETOTPS) algorithm, based on the knowledge of the C/N ratio at the receiver. In particular, we take the approach of adjusting link parameters like transmitter power and transmission mode to achieve the maximum throughput at different C/N values. Since the TOTPS algorithm tends to reduce the transmitter power without degrading the link throughput, transmitter power can be saved. This not only prolongs battery life, which is critical in ad hoc wireless networks, but also reduces the potential interference to neighboring wireless network systems. The ETOTPS algorithm, on the other hand, aims for higher throughput by trading in more transmitter power. This is particularly desired for high-speed data transfer in an emergency situation. Both algorithms are developed to be applied to IEEE 802.11b, IEEE 802.11a and IEEE 802.11g links. / Ph. D.

International Telemetering Conference Proceedings / October 29-November 02, 1990 / Riviera Hotel and Convention Center, Las Vegas, Nevada / Instrumentation for taking dynamic in-bore measurements during high accelerations typically has been limited to accelerations under 20,000 g’s. In munition development and testing, there is a need for telemetry instrumentation that can relay dynamic performance data at 100,000 g’s. This paper describes the development and testing of a stable, regulated, telemetry transmitter that has been successfully tested to 67,400 g’s.