Corn (Zea mays L.) and soybean (Glycine max L.) production in the Midwest US can result in significant nutrient leaching to groundwater and surface waters, which contributes to eutrophication and hypoxia in the Gulf of Mexico. A promising strategy to control nutrient leaching and sediment runoff loss during winter fallow period is the use of cover crops (CCs). In southern Illinois, CCs are not widely adopted by farmers due to economic constraints and the lack of scientific data that supports benefits of incorporating CCs into the corn-soybean rotation. Therefore, this doctoral dissertation addresses the critical question of the feasibility of the use of CCs in southern Illinois and is divided into three overarching research studies with different objectives divided into six research chapters. Research study 1 was a field experiment conducted from 2013 to 2017 to examine the effect of CCs (CC vs noCC) under two tillage systems [(no-tillage (NT) and conventional tillage (CT)] on aboveground plant attributes [dry matter yield, C:N ratio and nitrogen uptake (N uptake)], crop yields, available soil N content and N leaching in the vadose zone. The experimental layout was a randomized design with three rotations including corn-noCC-soybean-noCC [CncSnc], corn-cereal rye (Secale cereale L.) –soybean-hairy vetch (Vicia villosa R.) [CcrShv], and corn-cereal rye-soybean-oats+radish (Avena sativa L. + Raphanus sativus L.) [CcrSor] and two tillage systems. Soil samples collected after corn or soybean harvest and CC termination were analyzed for standard soil fertility parameters. Pan lysimeters installed below the ‘A’ horizon with depth varying from 22 to 30 cm were used for measuring soil solution nutrient concentration on weekly or biweekly basis depending on the precipitation. In NT system, the corn yield was 14% greater with CcrShv compared to CncSnc, whereas no significant difference existed in corn yield due to CC treatments within CT. Both CC treatments under NT reduced soybean yield by 24 to 27% compared to noCC. The rotations CcrShv and CcrSor with hairy vetch and oats+radish as preceding CCs resulted in 89% (37.73 vs 19.96 kg ha-1) and 68% (33.46 vs 19.96 kg ha-1) more nitrate-N (NO3-N) leaching than the CncSnc during cash crop season 2015. During the CC season in spring 2016, cereal rye CC in CcrShv and CcrSor reduced the NO3-N leaching by 84% (0.68 kg ha-1) and 78% (0.63 kg ha-1) compared to the CncSnc, respectively, under the CT system. Overall, our results indicated that the CT system had greater N leaching losses compared to NT system due to higher N availability in the tilled soil profile. The goal of the second research study was to understand the mechanisms of N cycling by CCs. Therefore, we applied 15N labeled urea fertilizer (9.2% atom) to corn that followed hairy vetch and noCC in May 2017 to evaluate the contribution of fertilizer and soil organic matter to N leaching and quantify the 15N content of surface runoff after storm events. During the 2017 corn season, repeated soil samples were collected and analyzed for 15N fertilizer recovery in soil at three depths. 15N recovery was higher in the corn that had hairy vetch as the preceding CC than the corn that had noCC by 13.13 and 3.68 kg ha-1 on soil sampling events of 7 and 21 days after planting of corn, respectively, at the depth 15-30 cm. Overall, the cumulative loss of 15NO3-N during corn season 2017 was <2% of the applied fertilizer. The contribution of NO3-N from soil organic matter to leaching was 61% higher for the corn rotation with hairy vetch CC compared to corn rotation with noCC (1.12 vs 0.69 kg ha-1). Research study 3 evaluated the effects of CCs (cereal rye and hairy vetch) and topography (shoulder, backslope, footslope) on corn-soybean production, soil and water quality, nutrient and sediment export in agricultural headwater streams in a paired-watershed experimental design. The crop rotation followed in the CC-watershed was corn-cereal rye-soybean-hairy vetch whereas the rotation in the noCC watershed was corn-noCC-soybean-noCC. Use of hairy vetch CC increased N uptake at shoulder, backslope and footslope positions by 110.90, 85.02, and 44.89 kg ha-1, respectively, when compared to noCC treatment. The corn yield at the shoulder position was increased by 69% in CC-watershed compared to noCC watershed in 2017 likely by providing large N additions following decomposition. Cereal rye increased soybean yield by 17% and 8% at the shoulder and backslope position, respectively in 2016. Additionally, the effects of topography and CCs on soil N levels and N leaching in the watersheds were also evaluated. The NO3-N concentrations measured using suction cup lysimeters in CC-watershed were reduced by 2.54 mg L-1 (67%) when compared to noCC watershed. During the hairy vetch CC season, the reduction in NO3-N concentrations in soil solution was only seen at the footslope position. The excessive N at footslope positions may have been immobilized or denitrified due to soil waterlogging from higher water availability at the footslope. Forty-two and 18 storm events were collected during a 4-yr calibration period and CC-treatment period, respectively. Predictive regression equations developed from the calibration period were used for calculating TSS, NO3-N, NH4-N, and DRP losses of surface runoff for the CC-treatment watershed. The CCs reduced TSS and discharge by 33% and 34%, respectively in the CC-watershed during the treatment period. However, EMCs for NO3-N, NH4-N, and DRP did not decrease. Overall, CCs are a recommended conservation practice for farmers who want to enhance the long term profitability of their production systems, while building soil health and protecting downstream water quality. The CCs have the potential to reduce nutrient leaching, peak/total discharge, improve soil quality and crop yields. However, reduction in leaching will depend on the type of CC (legume vs non-legume crops) used in rotation, the time of termination of CCs, cover crop establishment and number of years under CCs. Synchronizing N availability from CCs to N uptake by cash crop is important for reducing nitrate leaching and increasing crop yields.

The diverse cropping system of eastern Virginia's coastal plain offers limited opportunity to establish winter annual cover crops (WCC) for nitrogen (N) scavenging. The winter fallow niche after double-crop or full-season soybean (Glycine max L. Merr.) encompasses the majority of acres left fallow. Our objective was to evaluate interseeded WCC N scavenging performance following soybean and N supplying capacity to subsequent corn (Zea mays L.). Field studies were conducted at four different locations in each of the two study years. The experimental design was split plot with cereal rye, hairy vetch, and RV mix WCC as main plots and ten fertilizer nitrogen (FN) rates in a factorial arrangement (0 and 45 kg FN ha-1 as starter; and 0, 45, 90, 135, and 180 kg FN ha-1 at sidedress) to corn as subplots. The highest N uptake for cereal rye at winter dormancy was 18 kg N ha-1, but the average was 6-7 kg N ha-1. At WCC termination average N uptake for cereal rye was 35 and 40 kg N ha-1 in 2013 and 2014, respectively. Average biomass dry matter (DM) at WCC termination for cereal rye, cereal rye + hairy vetch mix (RV mix), and hairy vetch was 2356, 2000, and 1864 kg ha-1 in 2013; and 2055, 2701, and 692 kg ha-1 in 2014, respectively. Average cereal rye N uptake was 35 kg N ha-1 in 2013 and 40 kg N ha-1 in 2014. Significant differences for residual soil nitrogen were most apparent for soil nitrate (NO3-N) at lower depths (15-30 and 30-60 cm) during WCC termination and in the upper 0-15 cm during corn growth stage (GS) V4 of both years. Corn grain yield plateau following hairy vetch WCC was 0.7 and 0.6 Mg ha-1 higher than when following cereal rye WCC at zero and 45 kg ha-1 starter FN, respectively. Average agronomic optimum FN rates (AONR) were 26 and 9 kg ha-1 lower following hairy vetch than cereal rye WCC at zero and 45 kg ha-1 starter FN, respectively. Estimated hairy vetch FN reductions by FN replacement and AONR difference methods were 48 and 18 kg FN ha-1 in plots receiving zero starter FN; and 58 and -43 kg FN ha-1 in plots receiving 45 kg ha-1 starter FN. In-season soil N tests did not offer adequate information in order to predict sidedress FN reductions. These findings suggest that cereal rye and RV mix have the potential to scavenge and conserve residual soil N and hairy vetch is more than capable to supply PAN to subsequent corn when interseeded into soybean. / Master of Science

The purpose of this study is to investigate the level and distribution of genetic variation in Astragalus jaegerianus by using molecular markers. The objectives of the study are: to estimate levels of genetic variation within and among populations of Astragalus jaegerianus; to test the hypothesis that levels and patterns of genetic variation in species of restricted ranges and few individuals is low and partitioned at the population level; and to discern whether, or how well, genetic partitioning of Lane Mountain milk vetch correlates with its geographic partitioning in the field.

Bibliography: leaves 151-166. This thesis describes the development of two novel and complementary analytical approaches for assaying cyanoalanine non-protein amino acids. These assays are used to determine the distribution of these compounds both within and between plants and to identify accessions of common vetch which contain low levels of the cyanoalanine non-protein amino acids in germplasm collections. These analytical tools are used to correlate toxicity observed in animal feeding experiments with the cyanoalanine content. This thesis covers also the first report of the use of diffuse reflectance using dispersive infrared spectrometry for the "in situ" quantification of specific organic components from plant tissue as well as the first use of micellar electrokinetic chromatography for the quantitative analysis of 9-fluorenylmethyl chloroformate (FMOC) derivatised and non-derivatised components of extracts from plant material.

Switchgrass, Panicum virgatum, is being developed as an economically and ecologically sustainable biomass crop. Nitrogen is considered one of the most limiting inputs of switchgrass. Alternatives to synthetic nitrogen fertilization may be nitrogen-fixing legumes interseeded into switchgrass. The objectives of this research were: (1) develop efficient legume management strategies for switchgrass production systems, (2) evaluate and identify cool and warm-season legumes that can be grown compatibly with switchgrass, (3) determine whether switchgrass yields are increased by legume N-fixation, and (4) determine N-fixation of common (Vicia sativa) and hairy vetch (Vicia villosa). This study examined the establishment and persistence of ten different legume species in ‘Alamo’, a lowland variety of switchgrass in two switchgrass production systems: a one-cut biomass harvest and a two-cut forage/biomass harvest. Cool-season legumes were alfalfa (Medicago sativa), arrowleaf clover (Trifolium vesiculosum), common vetch, crown vetch (Securigera varia), red clover (Trifolium pretense), hairy vetch, and crimson clover (Trifolium incarnatum). Warm-season legumes were Illinois bundle flower (Desmanthus illinoensis), trailing wild bean (Strophostyles helvula), and partridge pea (Chamaechrista fasciculata). Red clover showed the highest plant densities and increase in switchgrass yields when interseeded into existing switchgrass stands in both harvest systems. Crude protein levels were highest in the 135 kg N ha-1 treatment in the forage cut of the two-cut harvest system. Arrowleaf clover, crimson clover, and red clover had high stand densities with annual reseeding. A combination of cool-season legumes, crimson clover and common vetch, in combination with warm-season partridge pea, were established in existing switchgrass stands after one year. Common vetch was evaluated for its nitrogen fixing capacity, seed germination, establishment, and effects on yield of switchgrass. Scarification by sulfuric acid had higher seed germination than other scarification treatments, except 100 grit sandpaper treatment for one minute at 0.7 kg of pressure. Common and hairy vetch nitrogen contributions were 59.3 and 43.3 kg N ha-1 respectively at seeding rates of 6.7 kg PLS ha-1. Switchgrass yields can increase with common and hairy vetch seeding rates of 7.6 and 10.4 kg PLS ha-1 to achieve 67 kg N ha-1, the recommended rate of N-fertilization for switchgrass stands.

Šio darbo tikslas buvo išanalizuoti ir įvertinti žirnių ir vikių mišinio įtaką pieno sudėčiai X ūkyje. Tikslui pasiekti buvo iškelti šie uždaviniai: 1. Išanalizuoti žirnių ir vikių mišinio įtaką pieno kiekiui ir sudėčiai. 2. Palyginti pieno sudėties rodiklius ganykliniu ir tvartiniu laikotarpiais. 3. Apibendrinti tyrimų rezultatus, pateikti išvadas ir pasiūlymus. Išvados: 1. Karvių, šertų žirnių ir vikių mišinio grūdų koncentruotaisiais pašarais, vidutinis pieno riebumas 0,08 proc.ir baltymingumas 0,04 proc. buvo mažesnis nei karvių, kurios buvo šertos visaverčiais kombinuotais pašarais. 2. Karvės, šertos visaverčiais kombinuotais pašarais, davė 0,023 kg pieno daugiau, nei karvės, šertos žirnių ir vikių mišinio koncentruotaisiais pašarais. 3. Laktozės kiekis piene buvo didesnis vidutiniškai 0,01 proc karvių , šėrtų žirnių ir vikių mišinio koncentruotaisiais pašarais. Laktozės kiekis statistiškai patikimas ( p<0,05). 4. Vidutinis urėjos kiekis abiejų grupių karvių piene buvo vienodas (18,77 mg/proc.). 5. Ganykliniu laikotarpiu 0,3 proc.(p<0,05) riebesnio ir 0,25 proc.(p<0,05) baltymingesnio pieno primelžta iš visaverčiais kombinuotais pašarais šertų karvių, o tvartiniu laikotarpiu 0,29 proc.(p<0,05) riebesnio pieno davė žirnių ir vikių mišinio miltais šertos karvės. / Tasks of work: overlook basis and ration of fodder given to milch-cows. Clarify how preparation of concentrated fodder, portioning out, and norming to the cows is managed. Identify what is the impact of concentrated mixture of vetch and bean stems to the cow’s productivity of milk composition. Generalize results of research, give conclusions and suggestions. Work placement and method. Work accomplished in X farm, in district of Rokiškis, duration 2011.11.01 – 2012.09.30. During the work: science literature was used, as well as statistical analysis, methods of data filing and summation. From results of research was found out that cows which were fed with concentrates of peas’s and vetch mixtures, average of milk fat 0.08% and proteins 0.04% was lower then cows which were fed full combined forage. Cows which were fed with complete combined forage gave 10.6kg more milk then those which were fed with concentrates of peas and vetch mixtures. Quantity of lactose in milk was higher average 0.01% of those cows which were fed with peas and vetch mixtures concentrates. Average urea quantity were equal (18,77 mg/%) in both cows groups. During pasturable period cows which were fed complete combined forage were milking 0.3% fatter and with more proteins 0.25% milk. During the cow-house period fatter milk 0.29% were given from cows which were fed of peas and vetch mixtures concentrates. In research it was observed lack of cellular tissue and protein in milk. Suggestion - pay... [to full text]

Bibliography: leaves 151-166. / xi, 166 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis describes the development of two novel and complementary analytical approaches for assaying cyanoalanine non-protein amino acids. These assays are used to determine the distribution of these compounds both within and between plants and to identify accessions of common vetch which contain low levels of the cyanoalanine non-protein amino acids in germplasm collections. These analytical tools are used to correlate toxicity observed in animal feeding experiments with the cyanoalanine content. This thesis covers also the first report of the use of diffuse reflectance using dispersive infrared spectrometry for the "in situ" quantification of specific organic components from plant tissue as well as the first use of micellar electrokinetic chromatography for the quantitative analysis of 9-fluorenylmethyl chloroformate (FMOC) derivatised and non-derivatised components of extracts from plant material. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1997

Thesis (Ph. D.)--University of Adelaide, Dept. of Plant Science, 1997. / Includes bibliographical references (leaves 151-166).

Thesis (doctoral)--Universität Leipzig, 1903. / Lebenslauf. Includes bibliographical references.

Current interest in cover cropping is focused on enhancing ecosystem services beyond soil conservation. Cover crop (CC) species function uniquely in their effects on ecosystem services when grown in monoculture or mixtures. This research integrated field experiments and a literature synthesis to evaluate the role of cover crops in improving nitrogen (N) management and simultaneously providing multiple ecosystem services. Legume CC fertilized with poultry litter (PL) could replace 101 to 117 kg N ha-1 of fertilizer in corn (Zea mays L.) production. Rye (Secale cereale L.) CC fertilized with PL had a negligible effect on corn production. Biculture fertilizer equivalence ranged between -12 to +75 kg N ha-1. Fertilizer equivalence of legume-containing treatments increased across time. Without CC, fall-applied PL failed to supply N to corn. Ecosystem services of CC and PL illustrate complex species functions. Bicultures produced more total biomass than monocultures in year 1 but less than rye in year 2. Bicultures were as effective in suppressing weeds as rye, produced corn yield similar to legume, and by the second year had similar amounts of available soil N as the legume. Poultry litter effects and interspecific effects cover crop species biomass differed. Rye yield increased, while legume yield decreased slightly in biculture. Poultry litter increased legume N content and a decrease in legume C:N, while rye N content and C:N were unaffected. The synthesis corroborates that mixed and biculture cover crops yield more than the individual component species. Overyielding was transgressive in 60% of cases studied. Mixture effects varied by species: rye and brassica yield increased, while legume decreased in mixtures. The effect of mixed CC on crop yields varied by crop species and management practices, though generally crops increased 8 to 18% overall. This work can be applied to the design of complex CC and PL systems that optimize individual species functions to enhance ecosystem services. / Ph. D.