Thesis (MSc (Psychology))--Stellenbosch University, 2008. / As part of the South African government’s strategy to support those with HIV/AIDS and their relatives, HIV/AIDS service points have been established at Public Health Facilities. An increasing number of HIV-positive children and their caretakers receive treatment here. Indirectly the paediatric patients’ uninfected caretakers and the adult patients’ children also come into contact with the Public Health System during visits. The HIV-positive and uninfected children and caretakers form a heterogeneous HIV/AIDS-affected population, which may experience particular fears due to their particular circumstances. Circumstance-particular coping strategies may be necessary. Children do, however, also experience normative childhood fears and their coping strategies display developmental characteristics. Direct and indirect learning experiences furthermore may contribute to children’s experience of fear. The participants were 40 HIV/AIDS-affected children (M = 9.4 years; HIV-positive = 30.0%) and their caretakers (N = 34; M = 36.7 years; HIV-positive = 76.5%) who had been in contact with the Public Health System of the Western Cape Province, South Africa. There were five sub-objectives. Regarding the children the objectives were to consider, against the background of normative literature, whether the target group was a special population regarding their (1) fears and (2) coping strategies. Furthermore the objective was (3) to get to know the children’s world through their learning experiences. Concerning the caretakers the objectives were to explore their personal (4) fears and (5) coping strategies. In addition the caretakers’ perceptions about their children’s fears and coping strategies were gathered. The study was explorative and a mixed methodological research model was used. Data were gathered through individual interviews. The children also completed the Goodenough-Harris Drawing Test (GHD) and the caregivers a demographic questionnaire. Data were analysed by using a combination of qualitative and quantitative strategies. The children reported normative childhood fears, mainly of wild animals. A substantial minority of the children reported fears connected to crime, an unsafe transport system, death, illness, and HIV/AIDS and special circumstances were therefore identified. The children’s coping strategies were circumstance-specific and problem-focused avoidance reported most. Living in a modern society these children’s fears have also been influenced by for instance television. The fears and coping strategies reported by the children and their care-takers’ perceptions thereof, concurred. The caretakers’ personal fears mainly were connected to psycho-social needs and social roles, which stand central during adulthood. Specifically fears about the children’s welfare were reported most. The caretakers reported crime and animalrelated fears similar to their children. A search for social support as well as preventative action were the coping strategies that were reported most. A noteworthy preventative-action strategy seemed to be healthy eating habits, given the controversy about natural products being used as HIV/AIDS treatment within the South African context. The findings were integrated within a systems-theoretical framework. The fears and coping strategies of the HIV/AIDSaffected children and their caretakers were interpreted to be the result of an interaction between their personal characteristics and their social environment.

Thesis (PhD (Plant Biotechnology))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: Virus Induced Gene Silencing (VIGS) was optimized to allow for the study of starch metabolism. The plastidial inorganic pyrophosphatase gene, for which a mutant has never been identified, was studied using VIGS and it was found to have a broad role in this subcellular compartment. The accumulation of inorganic pyrophosphate limited the production of starch, carotenoids, chlorophyll, and increased the plants susceptibility to drought stress. These effects highlight the importance of this enzyme in maintaining a low intraplastidial concentration of PPi providing an environment which facilitates these anabolic processes. Several genes involved in starch synthesis and degradation were also targeted with the aim of establishing a system of multiple gene silencing for the study of metabolic pathways. One, two and three genes were successfully silenced using this system which was validated based on previously published data. Interestingly, simultaneous silencing of the two isoforms of disproportionating enzyme led to a novel phenotype as a large reduction in starch instead of the expected increase was observed. / No Afrikaans abstract available

The positive diagnosis of HIV impacts the lives and the relationships of both people living and HIV and AIDS (PLHA) and their partners. This could be the result of the PLHA experiencing stigmatisation from the side of his or her partner, or associated stigmatisation experienced by the partner due to living with the PLHA. The literature review makes it clear that limited research has been conducted on interventions to reduce HIV stigma in PLHA and their partners and this subsequently forms the focus of this study. The study describes the effect of a community-based HIV stigma reduction intervention on PLHA and their partners through a case study method. In addition, it explores and describes the lived experiences of these two groups during and after an intervention in both an urban and a rural setting of the North West Province, South Africa. A qualitative holistic multiple case study design and interpretive descriptive approach through in-depth interviews was used to implement and evaluate the intervention. Purposive voluntary sampling was conducted to access PLHA, and snowball sampling to reach their partners. The case record included multiple sources. Document analysis and open coding was used for analysis of the case records and open coding for the in-depth interviews. No real differences were noted between the urban and rural groups. It was evident from both the case study and the expressed experiences of participants that the intervention was successful. Follow-up interventions with PLHA and partners are suggested, as well as implementations that involve other cultural groups in other provinces of South-Africa. Such implementations could be useful to evaluate the sustainability of the intervention in various cultural groups. The intervention could further be included in educational programmes offered in health care settings or NGO‘s to nurses, health care workers and volunteers to train people in HIV-stigma reduction programmes. / MCur, North-West University, Potchefstroom Campus, 2014

No description available.

The positive diagnosis of HIV impacts the lives and the relationships of both people living and HIV and AIDS (PLHA) and their partners. This could be the result of the PLHA experiencing stigmatisation from the side of his or her partner, or associated stigmatisation experienced by the partner due to living with the PLHA. The literature review makes it clear that limited research has been conducted on interventions to reduce HIV stigma in PLHA and their partners and this subsequently forms the focus of this study. The study describes the effect of a community-based HIV stigma reduction intervention on PLHA and their partners through a case study method. In addition, it explores and describes the lived experiences of these two groups during and after an intervention in both an urban and a rural setting of the North West Province, South Africa. A qualitative holistic multiple case study design and interpretive descriptive approach through in-depth interviews was used to implement and evaluate the intervention. Purposive voluntary sampling was conducted to access PLHA, and snowball sampling to reach their partners. The case record included multiple sources. Document analysis and open coding was used for analysis of the case records and open coding for the in-depth interviews. No real differences were noted between the urban and rural groups. It was evident from both the case study and the expressed experiences of participants that the intervention was successful. Follow-up interventions with PLHA and partners are suggested, as well as implementations that involve other cultural groups in other provinces of South-Africa. Such implementations could be useful to evaluate the sustainability of the intervention in various cultural groups. The intervention could further be included in educational programmes offered in health care settings or NGO‘s to nurses, health care workers and volunteers to train people in HIV-stigma reduction programmes. / MCur, North-West University, Potchefstroom Campus, 2014

The Southern parts of Africa have the highest prevalence of HIV-infected people and South Africa is the country with the highest number of infections in the world. There is still no cure for AIDS, but anti-HIV medicine can prolong and enhance the quality of life of an HIV infected person. Patient adherence with antiretroviral therapy is extremely low due to difficult dosing intervals, problematic dosage forms, instability of the antiretrovirals (ARVs) and the severe side-effects caused by these drugs; this leads to resistance of HIV to these drugs. Pheroid™ technology is a patented delivery system. Pheroid™ vesicles were used during this study. The entrapment of an active within the Pheroid™ would generally provide a safer, more effective formulation than the active alone. This could mean that the amount of drug needed for treatment of HIV can be decreased while producing fewer adverse effects and reducing the price of treatment. The main objectives of this study were to optimise and validate the cell viability and viral replication assays that can be used in an in vitro viral infection model. The MTT assay was used to asses the viability of the cells and to determine the toxicity of the antiretroviral drugs and Pheroid™ on the cells. HIV-1 assays were evaluated and used to determine the viral replication in the cells. Two different continuous cell lines were chosen for this study, an anchorage dependent GHOST cell line and suspended M7-Luc cells. Both these cell lines were best infected with the SWl virus. SWl is a subtype C, CXCR4 utilising virus. Subtype C is responsible for 60 % of the HIV infections worldwide and is the prevalent subtype in SUb-Saharan Africa .. Infection enhancers were not added to the cells to improve viral infection since it was observed that the Pheroid™ in combination with DEAE-dextran or Polybrene caused cytotoxicity probably by disrupting the cell's membrane. Antioxidants were added to the Pheroid ™ formulation since it was observed that the viability of the cells incubated with the Pheroid™ decreased as the Pheroid ™ matured. The added antioxidants had no significant effect on the cells. Abacavir (ABC) was chosen as the test substance for this study since it showed low cytotoxicity in cell cultures and is water soluble and would not present solubility issues in the media. It was entrapped within the Pheroid™ and its in vitro efficacy and toxicity was tested on HIV-infected and uninfected cell cultures. One directlHIV-specific (p24 antigen ELISA assay) and one indirect (Luciferase) assays were used to asses the inhibition of HIV replication caused by ABC. The p24 antigen ELISA (Enzyme-Linked ImmunoSorbent Assay) assay required a lot of washing steps and were rather expensive to use. The Luciferase assay was only used on the M7-Luc cells; this assay was sensitive, inexpensive and easy to use. The MTT (3-(4,5-demethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) viability assay was used to measure the toxicity caused by the Pheroid ™ and/or ABC on the cells. MTT is a widely used quantitative colorimetric assay to measure the viability of cells. The vitamin E and antioxidants contained in the Pheroid ™ reduced the MTT and produced results that were misinterpreted as enhanced viability when the Pheroid™ was present during MTT analysis. To prevent this problem an additional washing step should be introduced prior to analysis to reduce the interference of the Pheroid ™ with analytical methods. In conclusion, the efficacy of ABC entrapped within the Pheroid™ is still inconclusive and further studies will have to be done. MTT should be used with care for viability analysis of cells incubated in the presence of Pheroid TM. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2009.

Globally, nurses' contribution to informed health policy decisions is limited, as there are many barriers to Nurse led change to successfully influence the HIV and AIDS policy process. In South Africa nurses at all levels of health care are not involved or consulted during the formulation of the HIV and AIDS workplace policy. This has led to concern about the absence of nurses at the policy table. This study forms part of a larger international study programme entitled: “Strengthening Nurses’ Capacity in HIV and AIDS Policy Development in Sub–Saharan Africa and the Caribbean”. This programme of international research aims to empower nurses to become involved in the policy process (formulation, implementation and evaluation) in order to strengthen health systems in the areas of HIV and AIDS care. Nurses' absence at the policy table prompted the researcher to explore and describe barriers to Nurse led change to influence HIV and AIDS workplace policy. Phase 1 of the research consisted of a literature review to identify barriers to Nurse led change to influence the HIV and AIDS workplace policy. Management's opinion about the human resource management capacity and problems experienced working in an HIV and AIDS environment was obtained through a quantitative and qualitative empirical method of data collection and analysis. Frontline nurses' perspective was obtained through qualitative interviewing to identify problems experienced with policy in an HIV and AIDS workplace environment. A mixedmethod triangulation research design was used to achieve the objectives of phase 1 of the study, and strategies applied included exploratory, descriptive and contextual designs. The analysis of the data contributed to the identification and classification of problems experienced by nurses to influence HIV and AIDS workplace policy at macro, meso and microlevel, resulting in the formulation of fifty–nine (59) concluding problem statements. These concluding statements formed the basis for the strategy development for Nurse led change to influence HIV and AIDS workplace policy, which was the only objective of the second phase of the research. The strategy for Nurse led change to influence HIV and AIDS workplace policy was developed by using a strategic process to determine the vision, mission, values, principles, assumptions, strategic objectives and functional tactics based on the concluding problem statements. Finally, the research was evaluated, limitations were identified and recommendations were formulated for practice, education, research and policy. / Thesis (Ph.D. (Nursing))--North-West University, Potchefstroom Campus, 2011.

The Southern parts of Africa have the highest prevalence of HIV-infected people and South Africa is the country with the highest number of infections in the world. There is still no cure for AIDS, but anti-HIV medicine can prolong and enhance the quality of life of an HIV infected person. Patient adherence with antiretroviral therapy is extremely low due to difficult dosing intervals, problematic dosage forms, instability of the antiretrovirals (ARVs) and the severe side-effects caused by these drugs; this leads to resistance of HIV to these drugs. Pheroid™ technology is a patented delivery system. Pheroid™ vesicles were used during this study. The entrapment of an active within the Pheroid™ would generally provide a safer, more effective formulation than the active alone. This could mean that the amount of drug needed for treatment of HIV can be decreased while producing fewer adverse effects and reducing the price of treatment. The main objectives of this study were to optimise and validate the cell viability and viral replication assays that can be used in an in vitro viral infection model. The MTT assay was used to asses the viability of the cells and to determine the toxicity of the antiretroviral drugs and Pheroid™ on the cells. HIV-1 assays were evaluated and used to determine the viral replication in the cells. Two different continuous cell lines were chosen for this study, an anchorage dependent GHOST cell line and suspended M7-Luc cells. Both these cell lines were best infected with the SWl virus. SWl is a subtype C, CXCR4 utilising virus. Subtype C is responsible for 60 % of the HIV infections worldwide and is the prevalent subtype in SUb-Saharan Africa .. Infection enhancers were not added to the cells to improve viral infection since it was observed that the Pheroid™ in combination with DEAE-dextran or Polybrene caused cytotoxicity probably by disrupting the cell's membrane. Antioxidants were added to the Pheroid ™ formulation since it was observed that the viability of the cells incubated with the Pheroid™ decreased as the Pheroid ™ matured. The added antioxidants had no significant effect on the cells. Abacavir (ABC) was chosen as the test substance for this study since it showed low cytotoxicity in cell cultures and is water soluble and would not present solubility issues in the media. It was entrapped within the Pheroid™ and its in vitro efficacy and toxicity was tested on HIV-infected and uninfected cell cultures. One directlHIV-specific (p24 antigen ELISA assay) and one indirect (Luciferase) assays were used to asses the inhibition of HIV replication caused by ABC. The p24 antigen ELISA (Enzyme-Linked ImmunoSorbent Assay) assay required a lot of washing steps and were rather expensive to use. The Luciferase assay was only used on the M7-Luc cells; this assay was sensitive, inexpensive and easy to use. The MTT (3-(4,5-demethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) viability assay was used to measure the toxicity caused by the Pheroid ™ and/or ABC on the cells. MTT is a widely used quantitative colorimetric assay to measure the viability of cells. The vitamin E and antioxidants contained in the Pheroid ™ reduced the MTT and produced results that were misinterpreted as enhanced viability when the Pheroid™ was present during MTT analysis. To prevent this problem an additional washing step should be introduced prior to analysis to reduce the interference of the Pheroid ™ with analytical methods. In conclusion, the efficacy of ABC entrapped within the Pheroid™ is still inconclusive and further studies will have to be done. MTT should be used with care for viability analysis of cells incubated in the presence of Pheroid TM. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2009.

Globally, nurses' contribution to informed health policy decisions is limited, as there are many barriers to Nurse led change to successfully influence the HIV and AIDS policy process. In South Africa nurses at all levels of health care are not involved or consulted during the formulation of the HIV and AIDS workplace policy. This has led to concern about the absence of nurses at the policy table. This study forms part of a larger international study programme entitled: “Strengthening Nurses’ Capacity in HIV and AIDS Policy Development in Sub–Saharan Africa and the Caribbean”. This programme of international research aims to empower nurses to become involved in the policy process (formulation, implementation and evaluation) in order to strengthen health systems in the areas of HIV and AIDS care. Nurses' absence at the policy table prompted the researcher to explore and describe barriers to Nurse led change to influence HIV and AIDS workplace policy. Phase 1 of the research consisted of a literature review to identify barriers to Nurse led change to influence the HIV and AIDS workplace policy. Management's opinion about the human resource management capacity and problems experienced working in an HIV and AIDS environment was obtained through a quantitative and qualitative empirical method of data collection and analysis. Frontline nurses' perspective was obtained through qualitative interviewing to identify problems experienced with policy in an HIV and AIDS workplace environment. A mixedmethod triangulation research design was used to achieve the objectives of phase 1 of the study, and strategies applied included exploratory, descriptive and contextual designs. The analysis of the data contributed to the identification and classification of problems experienced by nurses to influence HIV and AIDS workplace policy at macro, meso and microlevel, resulting in the formulation of fifty–nine (59) concluding problem statements. These concluding statements formed the basis for the strategy development for Nurse led change to influence HIV and AIDS workplace policy, which was the only objective of the second phase of the research. The strategy for Nurse led change to influence HIV and AIDS workplace policy was developed by using a strategic process to determine the vision, mission, values, principles, assumptions, strategic objectives and functional tactics based on the concluding problem statements. Finally, the research was evaluated, limitations were identified and recommendations were formulated for practice, education, research and policy. / Thesis (Ph.D. (Nursing))--North-West University, Potchefstroom Campus, 2011.

The study is aimed at looking at the emotional needs the HIV/Aids affected coloured child in middle childhood. An in depth literature study was done and shortcomings have been identified with regard to the HIV/Aids affected child, in specific the experience of the child within the period before the loss of a parent because of HIV/Aids. A number of objectives were set in order to reach the goal of this study. By the consultation of literature and experts working in the field of HIV/Aids affected children in middle childhood, a theoretical framework was set up which included the following aspects: HIV/Aids in Sub Sahara Africa, the impact of HIV/Aids on the South African society, the HIV/Aids infected and –affected child, the impact of HIV/Aids on the family, the psycho social-, emotional- and social development of the child in middle childhood and cultural diversity in South Africa (with specific reference to the coloured culture and counselling for these children). During an empirical study, semi-structured interview techniques were applied as a method for data collection in order to assess the emotional needs of the HIV/Aids affected coloured child in middle childhood. Interviews focused on the HIV/Aids child and the following aspects were handled: the child’s wishes at that specific time in life (excluding the parent’s HIV/Aids status), the child’s experience of other’s interest in his own well being, the duration of the child’s knowledge of the parent’s HIV/Aids status, concerning changes (at school or at home) in the life of the child due to the parent’s HIV/Aids status, the child’s future custody and his feelings and ideas about it, individuals whom this child can talk to about his feelings of distress/anger/hurt, the child’s feelings about the knowledge concerning the parent’s HIV/Aids status and the child’s experience of newly acquired responsibilities at home (or towards his siblings) since the parents were HIV/Aids infected. Applied research was undertaken to aid the researcher in enhancing the awareness of professionals in practice with regard to the emotional needs of the affected coloured children in middle childhood. The professionals can then be empowered to understand and handle the problems which can be caused by the unfulfilled emotional needs of the HIV/Aids affected children. A qualitative research approach was used in gathering data, in order to get a full understanding of the emotional needs of the HIV/Aids affected child. A phenomenological strategy within an exploratory study was used aiming to understand and interpret the research question. The research question for this study is: “What are the emotional needs of the HIV/Aids affected coloured child in middle childhood?” Empirical data was obtained by means of an interview schedule and verified the following: -- The HIV/Aids affected coloured child in middle childhood experiences the need for emotional safety, which is threatened by the knowledge of the possibility of the death of a parent, and insecurity considering their future custody. The child is experiencing a variety of emotions of which bereavement is the most common one. The child’s emotional safety is also threatened by other factors associated with the parent’s HIV/Aids status. -- The HIV/Aids affected coloured child in middle childhood expresses the need to debriefing. It is common that these children do not have someone to share emotions with. Proof of above mentioned assumption can be ascribed to three factors namely: the people involved are not aware of the need for debriefing, the perception that it is better for the child not to express their emotions regarding the parent’s HIV/Aids infection and last, the lack of skills on ‘know how’ in approaching and handling this specific situation. -- The HIV/Aids affected coloured child in middle childhood experiences intense emotions concerning the parent’s HIV/Aids status whereas bereavement, concern and sympathy are identified. A few of these children are partly in denial about their parents’ HIV/Aids status and claim that the parents will die because of Tuberculoses. A number of these children totally deny the fact that their parents are sick. To assist the child in handling the related emotions and the traumatic situation, it will be beneficial to get therapeutic support. -- The HIV/Aids affected coloured child in middle childhood experiences drastic change when the parent’s HIV/Aids status is disclosed, or the moment the child starts to realise that something is wrong. The HIV/Aids parent is concerned about the impact of disclosure on the child and therefore delays this process. When the parent’s HIV/Aids status is disclosed, the child experiences a crisis and does not have the inner strength or required skills to handle his emotions and the situation which he is confronted with. -- The HIV/Aids affected coloured child in middle childhood experiences a need for acceptance by peer group members and therefore fear that they will discover their parent’s status. It became clear that in cases where the peers have found out about the HIV/Aids infected parent, their reactions were negative. Because of this, the HIV/Aids affected child experiences anger and grief. -- The HIV/Aids affected coloured child in middle childhood experiences a lack of concentration at school, which has a direct negative impact on his school performance. School attendance is also influenced negatively because of the other children’s negative attitude towards them. Seeing that school, as a formal institution, will influence the child’s social-, emotional- and psycho social development, it is of importance that the HIV/Aids affected child is supported in his scholastic functioning. This study exposed the emotional needs of the HIV/Aids affected coloured child in middle childhood which holds possibility for future research. The information collected can be utilized for further studying purposes and the intervention of children in similar situations. / Dissertation (MSD (Play Therapy))--University of Pretoria, 2006. / Social Work / unrestricted