Text in Afrikaans / Weens die veeleisende aard van sorggewing aan VIGS-weeskinders, bevind die sorggewers hulle dikwels in 'n posisie waar hulle self sorg en ondersteuning nodig het. Die vraag het begin ontstaan op watter manier hierdie sorggewers ondersteun kan word. Dit het duidelik geword dat die kerk vanuit hul sosiale verantwoordelikheid sorg en ondersteuning aan die sorggewers kan bied. Sorggewers van een instansie wat aan die navorsingsreis deelgeneem het, het inderdaad nie genoeg sorg en ondersteuning van die kerk ontvang nie. Hierdie gebrek aan ondersteuning het 'n direkte invloed op die sorggewers se hantering van sorggewingseise. Sorggewers van die ander twee deelnemende instansies ontvang genoeg ondersteuning van lidmate, en dit maak 'n groot verskil aan hoe sorggewingspanning beleef word. In hierdie studie is daar krities gekyk na wyses waarop die kerk betrokke is en verder kan betrokke raak by die sorggewers van VIGSweeskinders. / Philosophy, Practical & Systematic Theology / M.Th. (Praktiese Teologie)

It is important to understand the underlying plant defense mechanisms in order to establish best strategies to reduce losses due to diseases in cereals. The current available information is mostly on model organisms and their plant-pathogen interactions. However, this study is focused on the identification of genes involved in the resistance mechanism of one of the most devastating diseases of wheat, yellow rust. The strategy undertaken was to use differential display method (DD) together with microarray technology, on yellow rust differential lines of wheat (Avocet-Yr1 and Avocet-Yr10) infected with the virulent and avirulent Puccinia striiformis f. sp. tritici races (Pst: PST17, PST45, 169E136 and 232E137) together with appropriate control infections. DD primer combinations of ninety allowed the detection of fourteen differentially expressed genes which were also confirmed by real-time QRT-PCR analysis. All of but one were found to be novel sequences in wheat genome. Among those, two very important genes were identified as full ORF including 5&rsquo / and 3&rsquo / end untranslated regions (UTR) / namely cyclophilin like protein (putative antifungal activity) and ubiquitin conjugating enzyme (E2). The sequence homology analysis of the cloned gene fragments reveled that the genes detected have roles in ubiquitinylation, programmed cell death (apoptosis), putative antifungal activities, disease resistance, pathogen related responses, including a few with no known function. In addition to DD analysis, using wheat Affymetrix &ldquo / GeneChip&rdquo / , we identified 93 differentially expressed ESTs of wheat in response to avirulent pathogen attack. We also investigated the differential expression profiles of wheat leaves during the virulent infections and determined 75 differentially regulated ESTs. 1Selected ESTs were further analyzed using QRT-PCR analysis and 15 were confirmed to be differentially regulated. For the further characterization of the identified genes, such as determination of their putative roles in disease response, functional studies have to be performed. For this purpose, BSMV (Barley Stripe Mosaic Virus) mediated virus induced gene silencing (VIGS) method is optimized in this thesis for wheat. We have successfully managed to silence the endogenous PDS gene (Phytoene desaturase) of wheat which can be used as a positive control for the monitoring of silencing of the genes we have identified. Our results show that BSMV mediated VIGS can be used efficiently and effectively to silence wheat genes that we identified through differential display and microarray analysis and can be used to study the functions of those genes

Barley and wheat are the two most important crop species in Turkey. Molecular studies for increasing crop yield of these species are very important for the economic benefits of Turkey. Powdery mildew and yellow rust are the two main pathogens, infecting barley and wheat, respectively in our country and causing a great amount of yield loss each year. Till now, classical genetics studies were performed in order to develop resistant barley and wheat cultivars, but these studies have not been succesful. Therefore, molecular plant-pathogen interactions studies are starting to become the new tool to fight against pathogens. In this thesis, two important aspects of plant microbe interactions were investigated. In the first part, the role of microRNAs (miRNAs) in powdery mildew-barley pathosysytem, and yellow rust-wheat pathosystem were studied. The expression levels of miRNAs and their putative targets were investigated via miRNA microarray analysis and qRT-PCR, respectively, in response to virulent and avirulent pathogen infections. These data were used to establish a new model for powdery mildew-barley and yellow rust-wheat pathosystems. In the second part, functional analysis of a novel F-box gene, which was a ZTL-type F-box, was performed by using Barley Stripe Mosaic Virus mediated Virus Induced Gene Silencing. This F-box gene (HvDRF) (Hordeum vulgare Disease Related F-box) was induced upon yellow rust infection and we studied its role in powdery mildew infection. The results confirmed HvDRF as a positive regulator of race specific immunity and enlarged the roles of ZTL-type F-box proteins to biotic stress responses.

The HIV epidemic does not only affect people living with HIV or AIDS but has a large impact on the children. They are being stigmatised by association. There is paucity in research on HIV stigma interventions. In addition, existing interventions aimed at reducing HIV stigma are not community based and very few are aimed at HIV-affected children. The purpose of this study was to assess the change on psychological well-being of HIV-affected children after a Community based HIV Stigma Reduction and Wellness Enhancement Intervention, and to explore and describe their experiences thereof. This study formed part of a larger Community based HIV Stigma Reduction and Wellness Enhancement Intervention project. A mixed method convergent parallel design involving quantitative and qualitative data collection, analyses and integration of findings was applied. The sample was drawn from populations in the greater Potchefstroom urban area and rural Ganyesa in the North West Province. The children as participants in this study were children of PLHA in the larger study and were recruited using snowball sampling (n=11) and were between the ages 15 and 21 years. The quantitative component utilised a one group pre-test-repetitive-post-test design which was analysed by using IBM SPSS (ver. 20) by comparing t-test scores and F-ratios in ANOVA. The qualitative component of the study employed a holistic multiple case study approach and qualitative interpretive description and data were analysed by using thematic content and document analyses. The results indicated no significant difference between the urban and the rural groups in the subscales and total scores of mental well-being. The results of the total scores projected that the participants’ mental health was in the region of moderately mentally healthy. The in-depth interviews confirmed the three dimensions of the subscales indicating that they have verbalised similar experiences to the itemised subscales of emotional, social and psychological well-being. The intervention was a meaningful experience to the children. They gained knowledge about HIV stigma and how to cope with it; as well how to build relationships amongst themselves and with the PLHA. They gained a better understanding of their parents suffering from HIV and other PLHA as well as support of one another being in this difficult situation. Conducting the project led to them becoming empowered to act as leaders in HIV stigma reduction. The results of the in-depth interviews showed that the children gained a greater awareness of the process of the stigma and experienced a general increase in their knowledge throughout the workshop and the project. They formed meaningful relationships with other children and deepened their relationships with their parents and other PLHA. The children were empowered through these interventions to advocate against HIV stigma despite the challenges they faced. They gained confidence and experienced personal growth through their participation in the project. It is recommended that the findings of the study be applied in education to raise awareness of HIV stigma among psychology students and for training of practicing psychologists on their role in reducing HIV stigma and enhancing well-being of the PLHA and those living close to them. It could also be meaningful if the intervention were to be used for practice purposes where support is given to the newly diagnosed PLHA and those associated with them. Further research can be done to test sustainability of the intervention in a different context and with a bigger sample. / Thesis (MA (Research Psychology))--North-West University, Potchefstroom Campus, 2013.

The HIV epidemic does not only affect people living with HIV or AIDS but has a large impact on the children. They are being stigmatised by association. There is paucity in research on HIV stigma interventions. In addition, existing interventions aimed at reducing HIV stigma are not community based and very few are aimed at HIV-affected children. The purpose of this study was to assess the change on psychological well-being of HIV-affected children after a Community based HIV Stigma Reduction and Wellness Enhancement Intervention, and to explore and describe their experiences thereof. This study formed part of a larger Community based HIV Stigma Reduction and Wellness Enhancement Intervention project. A mixed method convergent parallel design involving quantitative and qualitative data collection, analyses and integration of findings was applied. The sample was drawn from populations in the greater Potchefstroom urban area and rural Ganyesa in the North West Province. The children as participants in this study were children of PLHA in the larger study and were recruited using snowball sampling (n=11) and were between the ages 15 and 21 years. The quantitative component utilised a one group pre-test-repetitive-post-test design which was analysed by using IBM SPSS (ver. 20) by comparing t-test scores and F-ratios in ANOVA. The qualitative component of the study employed a holistic multiple case study approach and qualitative interpretive description and data were analysed by using thematic content and document analyses. The results indicated no significant difference between the urban and the rural groups in the subscales and total scores of mental well-being. The results of the total scores projected that the participants’ mental health was in the region of moderately mentally healthy. The in-depth interviews confirmed the three dimensions of the subscales indicating that they have verbalised similar experiences to the itemised subscales of emotional, social and psychological well-being. The intervention was a meaningful experience to the children. They gained knowledge about HIV stigma and how to cope with it; as well how to build relationships amongst themselves and with the PLHA. They gained a better understanding of their parents suffering from HIV and other PLHA as well as support of one another being in this difficult situation. Conducting the project led to them becoming empowered to act as leaders in HIV stigma reduction. The results of the in-depth interviews showed that the children gained a greater awareness of the process of the stigma and experienced a general increase in their knowledge throughout the workshop and the project. They formed meaningful relationships with other children and deepened their relationships with their parents and other PLHA. The children were empowered through these interventions to advocate against HIV stigma despite the challenges they faced. They gained confidence and experienced personal growth through their participation in the project. It is recommended that the findings of the study be applied in education to raise awareness of HIV stigma among psychology students and for training of practicing psychologists on their role in reducing HIV stigma and enhancing well-being of the PLHA and those living close to them. It could also be meaningful if the intervention were to be used for practice purposes where support is given to the newly diagnosed PLHA and those associated with them. Further research can be done to test sustainability of the intervention in a different context and with a bigger sample. / Thesis (MA (Research Psychology))--North-West University, Potchefstroom Campus, 2013.

An estimated 30 to 36 million people worldwide are living with the Human Immunodeficiency Virus (HIV). In 2009 about 5.7 million of the 30 to 36 million people who are infected with HIV were living in South Africa, making South Africa the country with the largest number of people infected with HIV in the world (UNGASS, 2010). Van Dyk (2008) states that HIV infection and Acquired Immunodeficiency Syndrome (AIDS) are accompanied by symptoms of psycho–social distress, but relatively little is known of the direct effect of HIV and AIDS on psychological well–being. The psychological distress is mainly due to the difficulties HIV brings to daily life and the harsh reality of the prognosis of the illness (Van Dyk, 2008). It is not clear whether people infected with HIV who are unaware of their HIV status show more psychological symptoms than people in a group not infected with HIV. The research question for the current study was therefore whether people with and without HIV infection differ in their psycho–social symptoms and strengths before they know their HIV status. Accordingly, the aim of this study was to explore the psychosocial health profiles of people with and without HIV and AIDS before they knew their infection status. A cross–sectional survey design was used for gathering psychological data. This was part of a multi–disciplinary study where the participants’ HIV status was determined after obtaining their informed consent and giving pre– and post–test counselling. This study falls in the overlap of the South African leg of the Prospective Urban and Rural Epidemiology study (PURE–SA) that investigates the health transition and chronic diseases of lifestyle in urban and rural areas (Teo, Chow, Vaz, Rangarajan, & Ysusf, 2009), and the FORT2 and 3 projects (FORT2 = Understanding and promoting psychosocial health, resilience and strengths in an African context; Fort 3 = The prevalence of levels of psychosocial health: Dynamics and relationships with biomarkers of (ill) health in the South African contexts) (Wissing, 2005, 2008) on psychological well–being and its biological correlates. All the baseline data were collected during 2005. Of the 1 025 participants who completed all of the psychological health questionnaires, 153 (14.9%) were infected with HIV and 863 were not infected with HIV (since the HIV status of nine of the participants was not known, they were not included in the study). In the urban communities 435 participants completed the psychological health questionnaires, of whom 68 (15.6%) were infected with HIV and 367 were not infected with HIV. In the rural communities, 581 participants completed the psychological health questionnaires, of whom 85 (14.6%) were infected with HIV and 496 were not infected with HIV. The validated Setswana versions of the following seven psychological health questionnaires were used: Affectometer 2 (AFM), Satisfaction With Life Scale (SWLS), Community Collective Efficacy Scale (CCES), Mental Health Continuum Short Form (MHC–SF), New General Self–efficacy Scale (NGSE), Sense of Coherence Scale (SOC) and the General Health Questionnaire (GHQ). Descriptive statistics were determined for all measures for all the participants with, and without HIV. Significant differences in psychosocial profiles among individuals with and without HIV and AIDS and also between those in the rural and urban areas were determined by means of t–tests and by a multivariate analysis of variance (MANOVA). Practical significance was determined by the size of the effects. The results for the entire group showed statistically significant differences between the two groups of participants who were infected with HIV and those not infected with HIV regarding their sense of coherence and their perspective on the community’s capacity to succeed in joint activities, but these differences were of only small practical significance. The HIV–infected participants in the urban areas displayed statistically and practically a lower sense of coherence and viewed themselves as less capable of meeting task demands in community contexts, than did the participants not infected with HIV. Though the participants not infected with HIV in the rural group had, statistically and practically, a significantly greater capacity to succeed in joint community activities than the participants infected with HIV, an interesting finding was that the participants infected with HIV experienced more positive affect than the participants not infected with HIV. The research showed that people with and without HIV infection differ in some respects in their psycho–social symptoms and strengths even before they are conscious of their HIV status. It is striking that the differences found on the psychological measures for the participants reflected a personal sense of social coherence and perspective on their community’s capacity to succeed in joint activities, which was lower in the case of participants infected with HIV, and might therefore have led to high–risk social behaviours and consequent infections. It might be that the participants with a relatively lower sense of social coherence, integration, and co–operation towards collectively achieving meaningful goals were more inclined to manifest behaviours that would lead to detrimental consequences (in this case HIV infection) for themselves and others. The higher level of positive affect in the rural group of the participants infected with HIV is still unexplained and requires further research. / Thesis (M.Sc. (Clinical Psychology))--North-West University, Potchefstroom Campus, 2011.

HIV/AIDS treatment is costly. Lesotho as a resource–limited country depends mostly on donor funding for HIV/AIDS treatment and care. Knowledge of how much was spent on treatment of HIV/AIDS was lacking. This leads to overstocking of some ART medicines resulting in expiry. Sufficient funds need to be secured for the treatment programme. The main objective of the study is to assess the cost of antiretroviral medication treatments, by specifically assessing the cost of antiretroviral regimens, antiretroviral side effects, and the cost of medicines used for prophylaxis and treatment of opportunistic infections as well as the cost of monitoring laboratory tests and dietary supplements. The study engaged both public and private ART clinics in the Maseru District in Lesotho. The study population consisted of 1 424 patients and study period was between 12 and 56 months from January 2004 to August 2008. Retrospective observational method was used. The cost for HIV/AIDS treatment comprised the cost of antiretroviral medicines and those used for their side effects, opportunistic infections (OI) prophylaxis and treatment, dietary supplements as well as monitoring laboratory tests. Prescribed daily dose (PDD) was used to calculate the cost of all the medicines used. To determine significant differences in average costs for various regimens d– values were used, while a cost/prevalence index was used to determine whether the cost was worth spending on the population or not. Cost–effectiveness ratio was also utilized in order to assess whether the cost born was worth the benefit. The main findings revealed that regimens 1a (stavudine/lamivudine/nevirapine) and 1c (zidovudine/lamivudine/nevirapine) were the least expensive (cost/prevalence index of 0.6 and 0.7 respectively). Regimens containing efavirenz were found to be more expensive than those containing nevirapine (cost/prevalence index of 1.2 and 1.7 respectively). When using d–values, there was a significant difference between the cost of regimens 1a and 1b, 1a and 1d, 1c and 1d and the information could be used for regimen switching decisions. Increase in CD4 cell count was more in stavudine–based regimens than in zidovudine–based regimens, which cost less per treatment. Cost effectiveness ratio was lower in 1a with R9.42/1cell/mm3 of CD4 cell count increase, and the highest was 1d with R31.77/1cell/mm3 of CD4 cell count increase. Therefore it was concluded that stavudine–based regimens are less costly as they have the lowest cost– effectiveness ratio in the Lesotho clinic environment. / Thesis (M.Pharm. (Pharmacy Practice))--North-West University, Potchefstroom Campus, 2011.

The objective of this study is to explore the impact of a social group work programme in the lives of adolescent orphans in foster care in the North–West province who are also affected by HIV and AIDS, and to enhance their social functioning. Keywords: Adolescent, foster care, foster parent, social work, social group work, empower, family, orphan, HIV and AIDS There are a growing number of children in need of care in South Africa who are placed with foster parents. The biggest reason for foster placement is due to the rising mortality rate of biological parents due to HIV and AIDS. Section A refers to the problem statement, research objectives, research procedures and research methodology. The limitations of the research are also investigated and the definitions of key words. The research was conducted in two phases. The needs assessment was conducted in phase one and the planning, implementation, and evaluation of the social group work programme in phase two. The problem statement in section A is based upon the following: It was found that insufficient research is being conducted on the problems and circumstances of adolescent orphans in South Africa. It is evident that the physical and emotional needs of adolescent orphans are not being fulfilled. The HIV and AIDS pandemic have a negative effect on the quality of daily life for orphans under foster care. The problem statement gave rise to five research aims. The overarching research design conformed to the Development and Utilization Research model. The model was divided into five phases that guided the research. The single–system design was used to gather quantitative and qualitative data. A literature study was conducted around the themes of foster care, foster parents, social group work, and circumstances of the foster care situation. Section B consists of the four articles that form the report on the research outcomes Each article is dealt with as a self–contained unit focusing on specific research objectives that were achieved via specific research methods. These methods were employed to collect the necessary data for the design and evaluation of the social group work programme. Article 1: The circumstances and needs of the adolescent orphan in foster care. The aim of this article is to identify the circumstances and needs through a self–designed and structured schedule. Thirty adolescent orphans in foster care from the researcher's case load were identified to take part in the research. Twenty adolescent orphans were selected by means of accidental sampling. Ten (N = 10) were selected for the experimental group and ten (N = 10) were selected for the control group. The results indicated the problems and needs experienced by adolescent orphans. These could be used for discussion during a social group work programme designed to provide knowledge, teach skills, and improve the social functioning of the group. Article 2: The role of the social worker during service delivery to adolescent orphans in foster care. The nature and extent of foster care in the North–West province was investigated by means of the completion of questionnaires. The role of the social worker regarding services to adolescent orphans in foster care was also investigated. A self–structured questionnaire was used for data collection purposes and 85 questionnaires were sent to social workers in the North–West province. The results show the state of existing programmes and the opinions of social workers on topics that could be included in a social group work programme. The data was used in Article 2. Article 3: A social work intervention programme for adolescent orphans in foster care. The programme was developed according to the data received from the 30 (N=30) adolescent orphans in foster care and the 85 (N=85) social workers in the North–West province. The programme was presented to 30 (N=30) adolescent orphans in foster care. Social group work was used as method to present the programme. The group gained knowledge and received skills training, both of which served to enhance their social functioning. Article 4: The evaluation of a social group work programme for adolescent orphans in foster care The purpose of this article is to evaluate the social group work programme. Three standardized measuring instruments were used. The Generalized Contentment Scale, the Index of Family Relations, and the Personal Self–esteem Profile were used. These instruments were used three times: once before intervention, once during intervention and once after intervention. The control group 10 (N=10) also completed the questionnaire three times but did not attend the social group. A self–developed questionnaire was completed before and after each session. A self–developed evaluation was completed after the final session to assess the effectiveness of the programme. Section C - Summary, conclusions and recommendations. Section D - contains the appendices with the results of the three standardized measuring instruments that were used for data collection. Section E - Contains the Bibliography. / Thesis (Ph.D. (Social Work))--North-West University, Potchefstroom Campus, 2012.

An estimated 30 to 36 million people worldwide are living with the Human Immunodeficiency Virus (HIV). In 2009 about 5.7 million of the 30 to 36 million people who are infected with HIV were living in South Africa, making South Africa the country with the largest number of people infected with HIV in the world (UNGASS, 2010). Van Dyk (2008) states that HIV infection and Acquired Immunodeficiency Syndrome (AIDS) are accompanied by symptoms of psycho–social distress, but relatively little is known of the direct effect of HIV and AIDS on psychological well–being. The psychological distress is mainly due to the difficulties HIV brings to daily life and the harsh reality of the prognosis of the illness (Van Dyk, 2008). It is not clear whether people infected with HIV who are unaware of their HIV status show more psychological symptoms than people in a group not infected with HIV. The research question for the current study was therefore whether people with and without HIV infection differ in their psycho–social symptoms and strengths before they know their HIV status. Accordingly, the aim of this study was to explore the psychosocial health profiles of people with and without HIV and AIDS before they knew their infection status. A cross–sectional survey design was used for gathering psychological data. This was part of a multi–disciplinary study where the participants’ HIV status was determined after obtaining their informed consent and giving pre– and post–test counselling. This study falls in the overlap of the South African leg of the Prospective Urban and Rural Epidemiology study (PURE–SA) that investigates the health transition and chronic diseases of lifestyle in urban and rural areas (Teo, Chow, Vaz, Rangarajan, & Ysusf, 2009), and the FORT2 and 3 projects (FORT2 = Understanding and promoting psychosocial health, resilience and strengths in an African context; Fort 3 = The prevalence of levels of psychosocial health: Dynamics and relationships with biomarkers of (ill) health in the South African contexts) (Wissing, 2005, 2008) on psychological well–being and its biological correlates. All the baseline data were collected during 2005. Of the 1 025 participants who completed all of the psychological health questionnaires, 153 (14.9%) were infected with HIV and 863 were not infected with HIV (since the HIV status of nine of the participants was not known, they were not included in the study). In the urban communities 435 participants completed the psychological health questionnaires, of whom 68 (15.6%) were infected with HIV and 367 were not infected with HIV. In the rural communities, 581 participants completed the psychological health questionnaires, of whom 85 (14.6%) were infected with HIV and 496 were not infected with HIV. The validated Setswana versions of the following seven psychological health questionnaires were used: Affectometer 2 (AFM), Satisfaction With Life Scale (SWLS), Community Collective Efficacy Scale (CCES), Mental Health Continuum Short Form (MHC–SF), New General Self–efficacy Scale (NGSE), Sense of Coherence Scale (SOC) and the General Health Questionnaire (GHQ). Descriptive statistics were determined for all measures for all the participants with, and without HIV. Significant differences in psychosocial profiles among individuals with and without HIV and AIDS and also between those in the rural and urban areas were determined by means of t–tests and by a multivariate analysis of variance (MANOVA). Practical significance was determined by the size of the effects. The results for the entire group showed statistically significant differences between the two groups of participants who were infected with HIV and those not infected with HIV regarding their sense of coherence and their perspective on the community’s capacity to succeed in joint activities, but these differences were of only small practical significance. The HIV–infected participants in the urban areas displayed statistically and practically a lower sense of coherence and viewed themselves as less capable of meeting task demands in community contexts, than did the participants not infected with HIV. Though the participants not infected with HIV in the rural group had, statistically and practically, a significantly greater capacity to succeed in joint community activities than the participants infected with HIV, an interesting finding was that the participants infected with HIV experienced more positive affect than the participants not infected with HIV. The research showed that people with and without HIV infection differ in some respects in their psycho–social symptoms and strengths even before they are conscious of their HIV status. It is striking that the differences found on the psychological measures for the participants reflected a personal sense of social coherence and perspective on their community’s capacity to succeed in joint activities, which was lower in the case of participants infected with HIV, and might therefore have led to high–risk social behaviours and consequent infections. It might be that the participants with a relatively lower sense of social coherence, integration, and co–operation towards collectively achieving meaningful goals were more inclined to manifest behaviours that would lead to detrimental consequences (in this case HIV infection) for themselves and others. The higher level of positive affect in the rural group of the participants infected with HIV is still unexplained and requires further research. / Thesis (M.Sc. (Clinical Psychology))--North-West University, Potchefstroom Campus, 2011.

HIV/AIDS treatment is costly. Lesotho as a resource–limited country depends mostly on donor funding for HIV/AIDS treatment and care. Knowledge of how much was spent on treatment of HIV/AIDS was lacking. This leads to overstocking of some ART medicines resulting in expiry. Sufficient funds need to be secured for the treatment programme. The main objective of the study is to assess the cost of antiretroviral medication treatments, by specifically assessing the cost of antiretroviral regimens, antiretroviral side effects, and the cost of medicines used for prophylaxis and treatment of opportunistic infections as well as the cost of monitoring laboratory tests and dietary supplements. The study engaged both public and private ART clinics in the Maseru District in Lesotho. The study population consisted of 1 424 patients and study period was between 12 and 56 months from January 2004 to August 2008. Retrospective observational method was used. The cost for HIV/AIDS treatment comprised the cost of antiretroviral medicines and those used for their side effects, opportunistic infections (OI) prophylaxis and treatment, dietary supplements as well as monitoring laboratory tests. Prescribed daily dose (PDD) was used to calculate the cost of all the medicines used. To determine significant differences in average costs for various regimens d– values were used, while a cost/prevalence index was used to determine whether the cost was worth spending on the population or not. Cost–effectiveness ratio was also utilized in order to assess whether the cost born was worth the benefit. The main findings revealed that regimens 1a (stavudine/lamivudine/nevirapine) and 1c (zidovudine/lamivudine/nevirapine) were the least expensive (cost/prevalence index of 0.6 and 0.7 respectively). Regimens containing efavirenz were found to be more expensive than those containing nevirapine (cost/prevalence index of 1.2 and 1.7 respectively). When using d–values, there was a significant difference between the cost of regimens 1a and 1b, 1a and 1d, 1c and 1d and the information could be used for regimen switching decisions. Increase in CD4 cell count was more in stavudine–based regimens than in zidovudine–based regimens, which cost less per treatment. Cost effectiveness ratio was lower in 1a with R9.42/1cell/mm3 of CD4 cell count increase, and the highest was 1d with R31.77/1cell/mm3 of CD4 cell count increase. Therefore it was concluded that stavudine–based regimens are less costly as they have the lowest cost– effectiveness ratio in the Lesotho clinic environment. / Thesis (M.Pharm. (Pharmacy Practice))--North-West University, Potchefstroom Campus, 2011.