Made available in DSpace on 2016-07-18T17:51:15Z (GMT). No. of bitstreams: 1 Dissertacao Marcia.pdf: 237755 bytes, checksum: cc3f62f324b3824c463dbc43ad5d87b2 (MD5) Previous issue date: 2007-10-19 / Stress could be defined as an external factor that could cause some disadvantageous influence over the plant. In tropical regions, crops suffer non biotic stress especially due to water deficit and high temperature. Cross tolerance allow the plants to be acclimated after a short exposition to a specific stress. The objective of this work was to verify the water stress tolerance during bean seed germination influenced by thermal shock induced cross tolerance. Seed of common bean cv IAPAR 81 were submitted to the imbibition in substrate paper with pure water at 20ºC for 24h. One third of the seeds were maintained in that temperature. One third were submitted to cold shock for 24h at 7ºC in the first assay and 13ºC in the second and the final part were submitted to heat shock for 24h at 38ºC and 33ºC as used for the cold shocks; in all experiments there was no change of substrate. Seeds submitted or not to shock were transferred to paper substrate simulating hydric potential of 0; -0.6; -0,9 and -1.2MPa, in the first experiment and 0;-0.3; -0.6; -0.9 and -1.2MPa in the second experiment using mannitol at: 0; 22.29; 44.58; 66.87 e 89.17 g.L-1. Treatments were evaluated by germination percentage, abnormal plants and dead seeds. Seedling evaluation was done with shoot, root and total dry matter and by relation of root/shoot. The best development, in water restriction in the early germination, was of that seeds that passed through a 7ºC or 33ºC during 24h, what allowed the affirmation that induced cross tolerance occurred and that could be induced in the beginning of the imbibition in bean seeds. / Estresse pode ser definido como um fator externo, que exerce influência desvantajosa sobre a planta. Em regiões tropicais, as culturas agrícolas sofrem estresse abiótico principalmente por períodos de deficiência de água e excesso de temperatura. A tolerância cruzada permite às plantas se aclimatarem a uma gama de diferentes estresses após exposição a um estresse específico. O objetivo deste trabalho foi avaliar a tolerância ao estresse hídrico durante a germinação das sementes de feijão sob influência da tolerância cruzada induzida por choque térmico. As sementes de feijão cultivar IAPAR 81 foram submetidas ao processo de embebição, em substrato papel-toalha umedecido com água pura sob temperatura de 20ºC por 24 horas. A seguir, parte foi mantida nessa temperatura e parte transferida para o choque frio por 24 horas a 7ºC no ensaio 1 e 13ºC no ensaio 2 e outra parte para o choque quente por 24 horas a 38oC no ensaio 1 e 33oC no ensaio 2, sem troca do substrato. Tanto as sementes que passaram pelo choque como as que não passaram (controle) foram transferidas para substrato papel simulando diferentes potenciais hídricos, 0; -0,6; -0,9 e -1,2MPa, no ensaio 1 e 0;-0,3; -0,6; -0,9 e 1,2MPa no ensaio 2, induzidos por manitol nas seguintes concentrações: 0; 22,29; 44,58; 66,87 e 89,17 g.L-1. Os tratamentos foram avaliados por meio da porcentagem de germinação, plântulas anormais e sementes mortas; avaliações do desenvolvimento como massa seca da parte aérea, massa seca de raiz, massa seca total e relação raiz/parte aérea. O melhor desempenho das sementes que passaram por choque, de 7oC por 24h ou de 33oC por 24h, à restrição hídrica no início do desenvolvimento, permite afirmar que ocorreu indução de tolerância cruzada e que esta pode ser induzida no início do processo de embebição em sementes de feijão.

Plants rely on diverse strategies to regulate water loss during drought. The phytohormone abscisic acid (ABA) is a critical mediator of stomatal closure during water stress in seed plants. Studies in conifers identified diverging strategies in long-term drought of ABA-mediated dynamics, particularly a peaking type dynamic during long term drought in some conifers. Few studies have reported this dynamic in angiosperms, and no study has revealed the mechanism driving declines in ABA levels as drought progresses in peaking type species. To understand peaking type dynamics, we exposed the model peaking type gymnosperm species <i>Callitris rhomboidea</i> and the highly drought resistant evergreen angiosperm <i>Umbellularia californica</i> to controlled long-term drought. We measured leaf water potentials (Ψ_l), stomatal conductance, ABA and the ABA catabolite phaseic acid (PA) levels in potted plants during a prolonged but non-fatal drought. We aimed to determine which of three potential drivers of peaking type dynamic were responsible for this response: (1) increased catabolism of ABA into PA at a threshold Ψ_l , (2) ABA export from the leaf is enhanced under drought, and (3) ABA biosynthesis ceases at a threshold Ψ_l. During long term drought, the evergreen angiosperm species <i>U. californica</i> demonstrated peaking type ABA dynamics like gymnosperms. In both species, PA levels did not increase significantly, in fact, PA levels tracked ABA levels, suggesting that ABA catabolism to PA may be a function of ABA levels. Girdling experiments to determine whether export from the leaf drove declines in ABA levels demonstrated that of the majority of ABA was likely converted to ABA glucose ester (ABA-GE), an inactive storage form of ABA, and exported from shoots during drought. Finally, by rapidly dehydrating branched collected at different timepoints during long-term drought we were able to determine that ABA biosynthesis is completely down regulated in leaves that have been dehydrated beyond leaf turgor loss point. The decline in ABA levels in peaking type species appears conserved across seed plants and is mediated by high export rates in the form of ABA-GE. Future work should assess a more diverse selection of species as well as study long-term drought in less tolerant species to test whether ABA biosynthesis is deactivated in all species once Ψ_ldeclines below turgor loss point.

Dans le contexte actuel de changement climatique, anticiper la réponse des écosystèmes forestiers face à un risque de sécheresse accrue constitue un enjeu socio-économique, écologique et scientifique majeur. Les îles antillaises au-delà de leur statut de « hot spot de biodiversité », se situent dans un « hot-spot climatique », particulièrement à même d’être affecté par la réduction des précipitations. L’archipel Guadeloupéen constitue un modèle de choix dans l’étude des réponses végétales aux conditions environnementales. Il abrite l’ensemble de la diversité des écosystèmes caribéens, distribués le long d’un gradient altitudinal et de pluviométrie. Aux deux extrémités de ce gradient se situent des formations forestières expérimentant des conditions environnementales très contrastées : la forêt sèche et la forêt de nuage. Le premier objectif de cette étude était de caractériser les stratégies de croissance et la réponse physiologique à la contrainte hydrique de 8 espèces d’arbres structurants la forêt sèche et la forêt de nuage de Guadeloupe. Dans un premier temps, nous avons quantifié in situ, à différentes saisons, un large panel de traits fonctionnels intervenant à différentes échelles (cellule, feuille, individu) reflétant les principales fonctions végétatives des arbres (croissance, photosynthèse, transport hydraulique). Cette approche a permis de dresser un portrait fin du fonctionnement hydrique et carboné des espèces. L’approfondissement de la caractérisation de l’architecture hydraulique des espèces a été réalisée ex situ à travers une analyse structurelle (anatomique, morphologique) et fonctionnelle (résistance du xylème à la cavitation et des feuilles au flétrissement) afin de préciser leur potentiel de résistance à la sécheresse. Cette approche des différentes composantes du fonctionnement hydrique, rarement mise en œuvre, a permis de cerner les réponses des arbres de façon intégrée. Une importante diversité fonctionnelle a été mise en évidence au sein de chaque écosystème. Les espèces de forêt sèche, subissant une contrainte hydrique saisonnière marquée, montrent une capacité de résistance à la contrainte hydrique supérieure à celle des espèces de forêt de nuage. Cependant, la coexistence de différentes stratégies phénologiques dans cet écosystème aboutit à une importante diversité de stratégies hydriques et de croissance. En forêt de nuage, la contrainte hydrique édaphique est rare mais les espèces sont à même de subir une contrainte hydrique liée aux conditions atmosphériques. Dans cet écosystème, une diversité de comportement dépendant de la capacité des espèces à maintenir leur fonctionnement en cas de contrainte atmosphérique a aussi été mise en évidence. Nous avons identifié des corrélations intervenant entre la croissance cambiale, la résistance à la cavitation, la résistance au flétrissement foliaire et ses mécanismes d’ajustements sous-jacents ainsi que la sensibilité stomatique. Ces relations ont matérialisé un continuum cohérent et robuste de réponses fonctionnelles. La forte diversité relevée au sein des deux écosystèmes a induit une mixité de répartition des espèces le long de ce continuum. Afin de simplifier et résumer cette diversité mise en évidence nous avons cherché à former des groupes d’espèces déterminés par un jeu de traits morphologiques faciles à acquérir pour 14 espèces de forêt sèche et de forêt de nuage. / In the current context of climate change, anticipate the response of forest ecosystems facing an increased risk of drought is a socio- economic, ecological and scientific major issue. The Caribbean islands, beyond their status as «biodiversity hot spot», are in a "climate hot-spot ", particularly likely to be affected by reduced rainfall. The Guadeloupe archipelago is a model of choice in the study of plant responses to environmental conditions. It harbors all the diversity of Caribbean ecosystems, distributed along an altitudinal gradient and rainfall. At both ends of this gradient fall forest formations with contrasting environmental conditions: dry forest and montane cloud forest. The first aim of this study was to characterize growth strategies and the physiological response to water stress of 8 species of trees structuring the dry forest and cloud forest of Guadeloupe. First, we quantified in situ, in different seasons, a wide range of functional traits occurring at different scales (cell, leaf, individual) reflecting the main vegetative functions of trees (growth, photosynthesis, water transport). The deepening of the characterization of the hydraulic architecture of the species was carried out ex situ through a structural (anatomical, morphological) and functional (xylem resistance to cavitation and leaves wilt) analysis to clarify their potential resistance drought. This approach has enabled the identification of fine portrait of the water and carbon-functioning in the species. An important functional diversity has been demonstrated within each ecosystem. Dry forest species experience a marked seasonal water stress, show a higher resistance to water stress than that of cloud forest species. However, the coexistence of different phenological strategies in this ecosystem leads to a wide diversity of water and growth strategies. In cloud forest, edaphic water stress is rare but the species are likely to suffer water stress due to atmospheric conditions. In this ecosystem, a behavioral diversity dependent of the species' ability to maintain their functioning during atmospheric strain was also highlighted. We identified correlations between cambial growth, cavitation resistance, leaf wilt resistance and stomatal sensitivity. These relationships have materialized a coherent and robust continuum of functional responses. The high diversity recorded in the two ecosystems induced a mix of species distribution along this continuum.To simplify and summarize this diversity we sought to form groups of species determined by a set of morphological traits easy to acquire for 14 species of dry forest and cloud forest. This method produced groups reflecting different strategies for carbon management, influenced by the phenology of species. In contrast, groups obtained did not reflect drought response of species.Finally, because the seedling stage is a primary stage in terms of natural regeneration potential of forests, we evaluated ex situ the resistance to drought of dry forest seedlings. Like the adults, the dry forest seedlings showed a variety of strategies to cope drought.

<p>Thermal and multispectral imagery can provide users with insights into the water stress status and evapotranspiration demand of crops. However, traditional platforms, such as satellites, for these thermal and multispectral sensors are limited in their usefulness due to low spatial and temporal resolution. Small unmanned aircraft system (UAS) have the potential to have similar sensors installed and provide canopy temperature and reflectance information at spatial and temporal resolutions more useful for crop management; however, most of the existing research on the calibration or the estimation of water status were established based on the satellite platforms either for the sensors calibration or water status quantification. There is, therefore, a need to develop methods specifically for UAS-mounted sensors. In this research, a pixel-based calibration and an atmospheric correction method based on in-field approximate blackbody sources were developed for an uncooled thermal camera, and the higher accurate vegetative temperature acquired after calibration was used as inputs to an algorithm developed for high-resolution thermal imagery for calculating crop latent heat flux. At last, a thermal index based on the Bowen ratio is proposed to quantify the water deficit stress in a crop field, along with this, a method for plot-level analysis of various vegetation and thermal indices have been demonstrated to illustrate its broad application to genetic selection. The objective was to develop a workflow to use high-resolution thermal and multispectral imagery to derive indices that can quantify crops water status on a plot level which will facilitate the research related to breeding selection.</p> <p>The camera calibration method can effectively reduce the root mean square error (RMSE) and variability of measurements. The pixel-based thermal calibration method presented here was able to reduce the measurement uncertainty across all the pixels in the images, thus improving the accuracy and reducing the between-pixel variability of the measurements. During field calibration, the RMSE values relative to ground reference targets for two flights in 2017 were reduced from 6.36°C to 1.24°C and from 4.56°C to 1.32°C, respectively. The latent heat flux estimation algorithm yields an RMSE of 65.23 W/m² compared with the ground reference data acquired from porometer. The Bowen ratio has a high correlation with drought conditions quantified using the soil moisture index, stomatal conductance, and crop water stress index (CWSI), which indicates the potential of this index to be used as a water deficit stress indicator. The thermal and multispectral indices on a plot level displayed will facilitate the breeding selection.</p>

In the Mediterranean area water is a scarce natural resource and periods of drought are frequent. It is then important to increase water use efficiency of irrigated crops. In order to achieve this, one promising option is regulated deficit irrigation (RDI). RDI consists in reducing water application during stages of crop development when yield and fruit quality have low sensitivity to water stress. Full irrigation is provided during the rest of the season to maintain production and fruit quality at adequate levels (Behboudian and Mills, 1997). In citrus, flowering and fruit set are sensitive periods to water restrictions, because water stress during this period increases fruit drop (Ginestar and Castel 1996). The more appropriate phenological period for applying water restrictions seems to be the summer period providing that water applications returned at full dosage sufficiently before harvest in order to allow for compensation in fruit growth (Cohen and Goell 1988). Previous work by González-Altozano and Castel (1999) showed the feasibility of applying RDI in 'Clementina de Nules' and identified threshold values of plant water stress that allowed water savings of about 10-20% without any detrimental effect on yield or fruit size. It would be desirable now to study the extrapolation of these results to commercial orchards of citrus and assess the use of RDI in different citrus cultivars. Two RDI strategies (RDI-1, irrigated at 50% of crop evapotranspiration (ETc) during summer and; RDI-2, irrigated at 35% ETc during the same period to RDI-1) will be compared with a control treatment irrigated at full requirements. As the level of water stress reached by trees is the important factor when RDI strategies are applied, the study of accurate water stress indicators for citrus is also needed. Thus, during the period of water restrictions the use of sap flow and canopy temperature measurements, obtained by thermal imaging or by means of fixed infrared thermometer sensors, will be assessed and compared to classical methods like stem water potential and stomatal conductance. / Ballester Lurbe, C. (2013). Regulated deficit irrigation in citrus: agronomic response and water stress indicators [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/28582 / TESIS / Premios Extraordinarios de tesis doctorales

<p>Climate change, increasing demand and overuse have resulted in water stress, a condition where available water resources are not enough to meet needs, in many parts of the US. At the same time, increasing seasonal precipitation, and more intensive management practices mean subsurface drainage is expanding in the Midwest, which has resulted in significant nutrient loading to water bodies and changes to the hydrologic balance of river basins. To ensure the sustainability of water resources, it is crucial to understand how much water available, and how agricultural water use is impacted by changing weather, stress and legal conditions. Water use systems must be properly managed to ensure prosperous agricultural production, and secure water resources. The overall goal of this research is to evaluate the sustainability of groundwater resources in the US through better quantification of surface and groundwater use and the interactions with agricultural water management practices.</p> <p>Watershed-scale measures of surface water and groundwater stress were calculated to explore the spatial and temporal variability of water stress in the US. Much of the Western US, which has been in a water stress situation for 35 years (1985-2015), is shifting to increasing surface water use, but both groundwater stress and surface water stress are continuing to rise. An increase in groundwater stress has also been observed in isolated watersheds in the eastern United States. The pattern of rising surface water stress corresponds more closely to changes in water use, whereas the pattern of rising groundwater stress corresponds to changes in water supply. It has been observed that those watersheds that experienced water stress in 1985 have responded by increasing their water withdrawals from other sources, but they have not decreased their agricultural water withdrawals.  </p> <p>The decision to invest in groundwater irrigation depends on physical water availability and cost, as well as the right to use the water, as determined by groundwater doctrines. Overall, groundwater withdrawals for irrigation have increased in humid and temperate regions, and irrigation per unit area has also increased across the US. At the same time, the fraction of irrigation coming from groundwater is growing, suggesting a potential shift in the preferred water source. This is especially true in states following absolute ownership, correlative rights, and reasonable use doctrine. Correlative Rights doctrine prevalent in the western Corn Belt and Absolute Ownership doctrine (Indiana) appears to encourage increasing groundwater withdrawals during times of stress. These states increase their irrigation rate and the fraction of groundwater withdrawals for irrigation when less surface water is available.</p> <p>Subsurface drainage is common practice across vast areas of the US’ Corn Belt. Subsurface drainage can provide a better growing environment for crops, removing the excess water from the root zone and improving the trafficability of the soil. However, by lowering the water table, drainage has the potential to reduce groundwater recharge. Controlled drainage allows the user to control the water table by adjusting the height of the outlet to limit the subsurface flow during the non-growing season. To better understand the mechanism of how agricultural drainage practices influence aquifer recharge, the VIC model was utilized to represent subsurface drainage and groundwater storage in the Upper Wabash River basin. When using controlled drainage, the rate of recharge increased compared to the rate of free drainage. The mean annual difference between free drainage and controlled drainage was 16 mm/year for the 30 years study period (1983 to 2012). Controlled drainage also contributes greater stream baseflow, increasing low flows relative to free drainage. Despite excess seasonal water that requires the use of subsurface drainage, it is important to note that there have been local conflicts caused by well failures, in Indiana and neighboring states during times of drought, so the local impact of agricultural practices on groundwater is important. This dissertation increases understanding of the impact of agricultural drainage practices on water resource availability. </p>

Modern tools, nontraditional datasets and a better understanding of the interaction between climate and ecohydrology are continuously being developed as today's society is in critical need for improving water management, predicting hydrometeorological hazards and forecasting future climate. In particular, the study of the intra- and inter-annual variations in grass productivity and evapotranspiration caused by variations in precipitation/soil moisture and other biophysical factors is of great significance due to their relation to future climatic changes. The research presented here falls in three parts. In the first part of the dissertation, a land use adaptable model, based on the superposition of the temperature and water vapor pressure effects, is proposed for the effective clear sky emissivity. Ground radiometer and meteorological data, applicable in the subtropical climate of Saint Johns River Water Management District, Florida, were utilized for the model development over the spring season of 2004. The performance of this model was systematically evaluated by pertinent comparisons with previously established models using data over various land covers. The second part of the thesis investigates the dynamics of evapotranspiration with respect to its significant environmental and biological controls over an unmanaged bahia grassland. Eddy correlation measurements were carried out at a flux tower in Central Florida over the annual course of 2004. The main focus was on the sensitivity of the water vapor flux to wetness variables, namely the volumetric soil water content and the current precipitation index. It was shown that the time scales involved with the dynamics of evapotranspiration were on the order of six days, suggesting that depletion of the soil moisture was mostly responsible for the temporal fluctuations in evapotranspiration. Finally, simple models for the Priestley-Taylor factor were employed in terms of water availability, and the modeled results closely matched the eddy covariance flux values on daily time scale during all moisture conditions. In the third part of this work, the partitioning between latent and sensible heat fluxes was systematically examined with respect to biophysical factors. It was found that the seasonal variations in leaf area index, soil water content and net radiation were reflected in a strong seasonal pattern of the energy balance. Calculations of the bulk parameters, namely Priestley-Taylor parameter and decoupling coefficient, indicated that evapotranspiration of this grassland was controlled by water supply limitations and surface conductance. At an annual basis, the cumulative evapotranspiration was 59 percent of the precipitation received at the site. The results of this research complemented with other studies will promote better understanding of land-atmosphere interactions, accurate parameterizations of hydroclimatic models, and assessment of climate impact of grassland ecosystems.

No description available.

Stormwater bioretention cells (BRCs) are a variety of green stormwater infrastructure with the potential to restore pre-urban water balance, provided they can be tailored to infiltrate and evapotranspire (i.e., harvest) urban runoff in proportions consistent with pre-urban hydrologic conditions. This paper evaluates their capacity to do so, focusing on evapotranspirative harvest, which is relatively understudied, and the capacity of CSR (Competitve, Stress-tolerant, and Ruderal) functional type to serve as an overarching framework characterizing the water use strategy of BRC plants. The goal is to determine if harvest (and therefore the ratio of urban runoff infiltrated to harvested; the I:H ratio) might be fine-tuned to meet pre-urban values in BRCs through informed manipulation of plant community composition. This study focuses on 3 critical plant water use traits, the turgor loss point, the point of incipient water stress, and maximum stomatal conductance. A global plant traits meta-analysis identified degree of plant competitiveness and stress tolerance as significant determinants of all three water use traits, with stem type (woody vs herbaceous) also being significant, but only for turgor loss point. Based on these results, six water use scenarios appropriate for plants with different CSR type/stem type combinations were developed. BRC plants spanning the range of CSR types necessary to actionize these scenarios were determined to be available in eight major climate zones of the coterminous US, suggesting that regulating plant water use in BRCs using CSR is likely feasible. Hydraulic simulations (Hydrus 1D) were conducted for each scenario in all eight climate zones and revealed significant differences in evapotranspirative harvest and I:H ratios in simulated BRCs. Competitive woody plants had the highest evapotranspiration and lowest I:H ratios; 1.5-1.8 times more evapotranspiration and a 1.6-2 times lower I:H ratio than stress tolerant herbaceous plants, on average, across climate zones. Despite these significant differences, no simulated BRC in any climate zone was capable of reproducing pre-urban I:H ratios, regardless of plant type. More water was infiltrated than harvested in all scenarios and climates with the inverse being true for all pre-urban conditions. This suggests that absent additional sources of harvest (e.g., use of BRC water for nonpotable purposes such as toilet flushing and outdoor irrigation, or adoption of novel BRC designs that promote lateral exfiltration, stimulating "extra" evapotranspiration from nearby landscapes), BRCs will be unable to restore pre-urban water balance on their own. If true, then using BRCs in combination with other green technologies (particularly those biased towards harvest), may be the best path forward for balancing urban water budgets. / Master of Science / Stormwater bioretention cells (BRCs) are a variety of green infrastructure designed to manage urban stormwater flows that can dramatically reduce the amount of stormwater that is rapidly (and unnaturally) conveyed from paved surfaces to ecosystems. Their ability to recreate natural flow conditions is dependent on them balancing rates of infiltration – slowly filtering water down to the water table – and evapotranspiration – letting plants capture and transpire water. This paper evaluates the extent to which different plant functional types (competitive, stress tolerant, and ruderal (weedy)) can be used to regulate this balance, bringing urban hydrologic conditions closer to pre-urban ones. Competitiveness and stress tolerance were found to significantly influence plant water use traits, as was plant stem type (woody vs herbaceous) to a lesser extent (i.e., managing water budgets using CSR functional type is theoretically possible). Published BRC vegetation guidelines in 8 major US climate zones were found to include both competitive and stress tolerant species (i.e., the range of functional types required to regulate BRC water balance exists, suggesting it is feasible). Finally, hydraulic simulations conducted under six plant water use scenarios (reflecting different CSR types and stem types) revealed significant differences in the ratio of water infiltrated to evapotranspired by BRCs (i.e., changing plant functional types can meaningfully influence BRC water balance). This said, the magnitude of this effect may be insufficient to return urban catchments to a pre-urban state. All BRCs infiltrated too much water in our simulations suggesting that absent additional sources of harvest (for instance., use of BRC water for nonpotable purposes such as toilet flushing or outdoor irrigation), BRCs will be unable to restore pre-urban water balance on their own. If true, then using BRCs in combination with other green technologies (particularly those biased towards harvest), may be the best path forward for balancing urban water budgets.

The work developed in this thesis aimed to increase understanding about the variability and stability in eleven biotypes of Pochonia chlamydosporia, a facultative parasitic fungus with potential as a biological control agent against root-knot (Meloidogyne spp.), false root-knot (Naccobus spp.) and cyst nematodes (Heterodera and Globodera, spp.). Differences in performance were assessed by measuring saprophytic and parasitic growth using in vitro bioassays. Information on virulence (in vitro) was collected for a range of biotypes with the objective to relate in vitro parasitic growth with rhizosphere colonisation ability and secretion of extracellular enzymes. Results showed differences between biotypes in their ability to colonise the rhizosphere of plants, parasitise nematode eggs and to produce a range of extracellular enzymes but no significant relationships were found between saprophytic or parasitic growth and enzyme production. For the first time, the specific activity of protease, chitinase, esterase and lipase enzyme production by eleven biotypes of the fungus was examined. Enzymatic activity was shown to vary with the biotype and type of enzyme assayed and biotypes could be ranked according to their similarities in enzyme production A novel bioassay to estimate egg parasitism using liquid media highlighted the importance of nutrition in infection processes and suggested that all biotypes are able to infect large numbers of eggs rapidly if the conditions are favourable. The assay reliably detected fungal infection in nematode eggs within 48 hours and provided a simple, rapid assay to test the effect of specific nutrients at controlled concentrations on the infection process. Differences in infection rates between biotypes observed in previous tests on agar were not detected in the new assay in which nematode eggs and fungal conidia were added in suspension. Internal colonisation of individual whole Meloidogyne spp. eggs by P. chlamydosporia was observed using microscopy studies. The destruction of nematode eggs infected with the fungus within seven days, was confirmed. The in vitro formation of appressoria was studied in a range of P. chlamydosporia biotypes. for the first time. Biotypes were found to differ in their ability to produce appressoria but this ability was not related to differences in virulence (in vitro) against nematode eggs. Cont/d.