Despite the ubiquity of wildlife management, from reintroductions and supplemental feeding to culling and habitat destruction, very little is known of the effects of management practices on species’ social dynamics. Species’ social structure has the potential to affect not only behaviour and evolution but also the transmission of information or disease. Understanding the effects of population management on social behaviour and organisation is a key step in understanding these species’ ecology. This thesis examines the differences between individuals’ roles in the social structure and what this means for the transmission of disease. It demonstrates how similarity in movement behaviour scales with increasing social circles, how seasonality in movement and seasonality in association rates covary as well as detailing post-cull behavioural changes. It finds that there is the potential for certain individuals (most likely non-breeding individuals) to transmit infection far and wide. It reveals the similarities in movement behaviour and body condition that birds share with their pair and social group. It emphasises the importance of autumn and winter movement in the transmission of infectious disease and it follows the short- and long-term changes in social structure and movement behaviour following a cull. Cull survivors were observed to retain a higher proportion of associations with their previous associates and moved less far in the year following the cull than in the year preceding it. This is the first application of social network analysis to quantify social structure before and after culling. The findings suggest that culling an infected population may facilitate rather than constrain the transmission of disease.

Effectively developing robust predictive models for forecasting infectious disease dynamics over space and time relies on successful surveillance strategies to accurately assess host infection status. We are constantly refining these models to improve our understanding of transmission and persistence dynamics in host populations but are continuously challenged with difficulties in accurately diagnosing host infection status. These challenges are especially persistent for pathogens of the Mycobacterium tuberculosis Complex (MTBC), which cause tuberculosis (TB) disease in a wide array of mammalian hosts. These challenges are further exacerbated when working with MTBC pathogens in free-ranging wildlife hosts. Although TB disease in humans is a primary concern, TB in free-ranging wildlife hosts poses a large threat to human and animal health. One recently described and novel MTBC pathogen is Mycobacterium mungi, which infects the highly social, group-living banded mongoose (Mungos mungo). M. mungi poses a large threat to human and animal health as banded mongoose hosts thrive in urbanized areas and live in close proximity to humans, but despite this threat, accurately diagnosing M. mungi infection status remains a primary challenge. Here, I develop a host response-based assay for differentiating banded mongoose with clinical M. mungi disease from individuals that are putatively healthy using transcriptional biomarkers in whole blood. To our knowledge, this is the first evaluation of host response-based transcriptional signatures to detect TB infection in unstimulated whole blood collected from a free-ranging wildlife species. I found that the expression of two genes, GBP5 and DUSP3, are significantly upregulated (GBP5, p < .05; DUSP3, p < .005) in banded mongoose with clinical M. mungi disease when compared to that of putatively healthy individuals. These results are consistent with studies of active M. tuberculosis disease in humans and support the use of host response-based assays using blood transcriptional biomarkers for diagnosing TB in free-ranging wildlife hosts. These findings are important for improving surveillance strategies for diagnosing M. mungi infection status in banded mongoose and will be essential in refining predictive models for forecasting transmission and persistence dynamics over space and time. / Creating models to predict how diseases circulate and persist within a population is dependent on our ability to accurately diagnose if a host is infected. Diagnosing infection is difficult for some diseases, including tuberculosis (TB) pathogens, which infect humans and many other mammalian species. While vast improvements have been made in diagnosing TB infection in humans, diagnosing TB in free-ranging wildlife species is a constant challenge. These challenges are further exacerbated across the different pathogen species of TB. Although TB disease in humans is a primary concern, TB in free-ranging wildlife hosts poses a large threat to human and animal health. One recently discovered TB pathogen is Mycobacterium mungi, which infects free-ranging banded mongoose (Mungos mungo). This pathogen poses a large threat to human and animals health since banded mongoose thrive in urbanized areas and live in close proximity to humans. Despite this threat, accurately diagnosing M. mungi infection in banded mongoose remains a challenge. Here, I develop a diagnostic molecular tool that uses banded mongoose blood to measure the expression of specific genes and differentiate diseased individuals from seemingly healthy individuals. To our knowledge, this is the first study that has used this specific approach for diagnosing TB in a free-ranging wildlife species. I found that the expression of two genes are significantly increased in banded mongoose with clinical M. mungi disease when compared to that of seemingly healthy individuals. These results are consistent with studies human TB disease in humans and support the use of this approach for diagnosing TB in free-ranging wildlife hosts. These findings are important for improving diagnostics for M. mungi infection in banded mongoose and will be essential in refining models for predicting how this disease circulates and persists over space and time.

The vectors of avian malaria (Haemosporida) are an understudied component of wildlife disease ecology. Most studies of avian malaria have focused on the secondary bird hosts. This imbalance leaves a significant gap in our knowledge and understanding of the insect hosts. This study investigates the diversity of malaria parasites carried by mosquitoes (Diptera, Culicidae) in the state of Mississippi. Using PCR techniques, haemosporidian infection rates were determined and parasites were identified in a phylogenetic context to those previously annotated. A total of 27,157 female mosquitoes representing 15 species were captured. Five of those species tested positive for malaria parasites with an overall infection rate of 4 per 1000 mosquitoes infected. Mosquitoes were shown to harbor Plasmodium and Haemoproteus parasites. Surprisingly, a unique lineage of parasites was discovered in Anopheles mosquitoes potentially representing a new genus of haemosporidian parasites, reinforcing the need to continue investigating this diverse group of parasites.

No description available.

Infectious disease transmission is driven by a complex suite of drivers with behavior and landscape dynamics contributing to epidemics across host-pathogen systems. However, our understanding of the interaction between landscape, behavior, and infectious disease remains limited. In the banded mongoose (Mungos mungo), a novel tuberculosis pathogen, Mycobacterium mungi, has emerged in Northern Botswana that is transmitted through olfactory communication behaviors. Using this host-pathogen system, this thesis explores the influence of various land use areas along the human-wildlife interface on animal behavior, and ultimately, pathogen transmission potential. Using behavior data from remote sensing camera traps, a generalized linear mixed model identified vigilance behavior, land use, and their interaction as important factors in predicting olfactory behavior. Cluster and Classification and Regression Tree (CART) analysis of active den sites (n= 308, across 23 troops) identified the important characteristics of dens across land use areas. In human-modified environments, man-made den sites persisted longer than did natural dens which became unsuitable through environmental processes (e.g., collapse). We also document the occurrence of nighttime activity for this species, perceived to be strictly diurnal. These data provide information critical to the development of robust computational models and underscore the importance of both landscape and behavior in accurately predicting and managing infectious disease outbreaks. / M. S. / The overall objective of this thesis was to identify the manner in which changing landscapes may influence disease transmission. Differences in host behavior across the landscape can influence pathogen transmission. Banded mongooses (Mungos mungo) are infected with a tuberculosis pathogen (Mycobacterium mungi) that is transmitted through olfactory secretions used for communication. We studied how olfactory communication is influenced by land type (i.e., lodge, national park, residential, undeveloped, and urban) and vigilance behavior. The vigilance behaviors that allow mongooses to avoid predators and competitors had a positive influence on olfaction behavior in some land use areas, but a negative influence on others. We also examined the characteristics of den sites used by banded mongooses in relation to different land use areas. Den sites in human-mediated land use areas were often man-made, remained in use for much longer than natural den sites, and were used by multiple troops. These findings suggest that landscape can be a driving force influencing behavior, and subsequently disease transmission. Understanding the influence of anthropogenically modified landscapes on disease transmission will be key to managing wildlife species.

Protected lands are an important source of food, shelter, and reproductive opportunities for wildlife, especially in urbanizing landscapes. When urban development abuts the edges of protected lands, synanthropic species can alter their foraging behaviors and movement to utilize human-supplemented resources throughout the urban-wild interface. Therefore, urban edges on protected lands can have pronounced effects on animal movement and ecosystem function. There is also increased risk of disease transmission where high densities of wild animals exist, or where domestic animals and wild species are interacting. In particular, members of the family Felidae, including domestic cats (Felis catus), can transmit the pathogenic parasite, Toxoplasma gondii (henceforth T. gondii) through their feces. Capable of infecting any warm-blooded host species, infection with T. gondii has been proven to cause increased risk-taking behaviors, stillbirths and congenital birth defects, and mortality in humans and a variety of wildlife species. Northern raccoons (Procyon lotor) and Virginia opossums (Didelphis virginiana) are urban adaptive species who often benefit from human-supplemented food sources such as unsecured garbage, and outdoor pet food, and may be exposed to T. gondii more frequently if they are utilizing resources close to cats. To investigate how urban edges of protected lands affect the movement and infection risk for urban-adapted mesopredators, I captured and attached GPS collars to opossums and raccoons between April 2022 and October 2023 in northern Key Largo, FL, USA, a protected area with large contiguous patches of undisturbed land cover adjacent to two distinct urban areas with high outdoor cat densities and available human-supplemented food resources. Using GPS collar data, I estimated home ranges and third-order resource selection of 27 raccoons and 12 opossums from Key Largo, FL. The average home range of opossums were 20.51 ha (14.12 SE) which was significantly smaller than raccoons (137.01 ha, 10.74 SE, t37 = -2.70, p = 0.01). The proportion of urban development in an individual’s home range was the most influential factor associated with home range size, followed by species and sex. Individuals with greater proportions of residential neighborhoods and commercial areas in their home ranges were associated with smaller home ranges. Third-order resource selection functions identified both mesopredator species using residential and commercial land use areas significantly more than they were available on the landscape. To survey for T. gondii infections, I collected blood samples from all the captured raccoons and performed a direct modified agglutination test (MAT) to detect antibodies for T. gondii. To understand how human-supplemented foods could be affected exposure rates, I collected hair samples from all raccoons and analyzed the stable carbon isotope ratios, so that higher ratios of carbon were equated to greater intake of non-natural, human-supplemented food resources. 67.7% (21/31; 95% CI: 51.3–84.2%) of the raccoons tested positive for T. gondii antibodies, and the proportion of urban development in an individual’s home range was the best predictor for infection. A multinomial regression analysis indicated that raccoons with higher levels of antibodies were also likely to have a majority “non-wild” diet based on δ13C values from a stable carbon isotope analysis. These results suggest the primary route of parasite transmission is contact with domestic cats, which may be exacerbated by the exploitation of human-supplemented food in urban areas. The Florida Keys are currently facing mammal population declines tied to Burmese pythons (Python bivitattus) and outdoor cat predations, and management policies that decrease the circulation of T. gondii oocysts in the environment are important to bolstering the fitness of these island-dwelling populations. Decreased reproductive success or behavioral changes that increase risk of predation could spell catastrophic for these mesopredator populations that are following in the footsteps of their Everglades counterparts, and at risk of extirpation on Key Largo. I recorded a systemic reduction of home range sizes on the urban edges of the protected areas of Key Largo, which often corresponds with higher densities of animals, which may increase probability of disease transmission, especially when the urban borders support populations of feral domestic species. As urbanization increases and the distance between wild lands and human disturbance decreases, it is increasingly important to study the mechanisms of how urban development on the edges of protected areas affect the ecology of wildlife species.

This thesis explores the evolution of avian influenza A viruses (IAV), as well as host-pathogen interactions between these viruses and their main reservoir host, the Mallard (Anas platyrhynchos). IAV is a genetically diverse, multi-host virus and wild birds, particularly dabbling ducks, are the natural reservoir. At our study site, up to 30% of migratory Mallards are infected with IAV during an autumn season, and host a large number of virus subtypes. IAV diversity is driven by two main mechanisms: mutation, driving genetic drift; and reassortment following co-infection, resulting in genetic shift.   Reassortment is pervasive within an autumn season, both across multiple subtypes and within a single subtype. It is a key genetic feature in long-term maintenance of common subtypes, as it allows for independent lineage turn-over, generating novel genetic constellations. I hypothesize that the decoupling of successful constellations and generation of novel annual constellations enables viruses to escape herd immunity; these genetic changes must confer antigenic change for the process to be favourable. Indeed, in an experiment utilizing vaccines, circulating viruses escaped homosubtypic immunity, resulting in the proliferation of infections with the same subtype as the vaccine. While the host plays an important role in shaping IAV evolutionary genetics, one must consider that Mallards are infected with a multitude of other microorganisms. Here, Mallards were infected with IAV, gamma coronaviruses, and avian paramyxovirus type 1 simultaneously, and we found a putative synergistic interaction between IAV and gamma coronaviruses.   Mallards occupy the interface between humans, poultry, and wild birds, and are the reservoir of IAV diversity. New incursions of highly pathogenic H5 viruses to both Europe and North America reaffirms the role of wild birds, particularly waterfowl, in diffusion of viruses spatially. Using European low pathogenic viruses and Mallard model, this thesis contributes to aspects of epidemiology, ecology, and evolutionary dynamics of waterfowl viruses, particularly IAV

The topic of badgers in the UK is often a contentious one, dividing opinions and sparking political debate. On one hand, badgers represent an important part of the British ecosystem but on the other a wildlife reservoir of disease implicated in the transmission of bovine tuberculosis (TB) to livestock in the UK. This has prompted strong interest in their population dynamics and epidemiology. Using data from a long-term study of a naturally infected badger population in Woodchester Park, Gloucestershire, this thesis explores a range of capture-mark-recapture (CMR) models to further understand disease and demographic processes. The first section examines long term population dynamics, simultaneously estimating demographic rates alongside their drivers using integrated population models (IPMs). The findings provide new insight into badger demography, highlighting density-dependent mechanisms, vulnerabilities to changing climate and disease prevalence and subsequently how multi-factorial analyses are required to explain fluctuating badger populations. The following sections use multistate models to answer pertinent questions regarding individual disease dynamics, revealing rates of TB infection, progression and disease-induced mortality. A key finding was sex-differences in disease response, with males more susceptible to TB infection. After applying a survival trajectory analysis we suggest sex differences are due to male immune defence deficiencies. A comparative analysis demonstrated similarities between epidemiological processes at Woodchester Park to an unconnected population of badgers from a vaccine study, supporting its continued use as a model population. The final study in this thesis constructs an IPM to estimate disease and population dynamics and in doing so uncovers disease-state recruitment allocation rates, demographic and population estimates of badgers in varying health-states and predicts future dynamics. This model aims to encapsulate the more commonly held notion of populations as dynamic entities with numerous co-occurring processes, opening up avenues for future analyses within both the badger-TB system and possible extensions to other wildlife reservoir populations.

For the second study, a web-based survey was developed and distributed to all members of four major health education organizations. A total of 1,925 HEs’ completed the survey and 1,607 responses were utilized in the final analysis. This study indicated that participants had deficient knowledge and unfavorable attitudes toward the CDCproposed genomic competencies. In the third study, a theoretical model was developed to predict HEs’ likelihood to incorporate genomic competencies into their practice. Using techniques from Structural Equation Modeling (SEM), the model was tested with the same data of the second study. Findings supported the proposed theoretical model. While genomic knowledge, attitudes, and self-efficacy were significantly associated with HEs’ likelihood to incorporate genomic competencies into their practice, attitudes was the strongest predictor of likelihood. In summary, these studies indicated that participating HEs had deficient genomic knowledge, unfavorable attitudes toward a set of CDC-proposed genomic competencies, and low likelihood to adopt genomic competencies into health promotion. Relevant training should be developed and advocated. As the SEM analysis results indicated the survey findings supported the proposed theoretical model, which can be utilized to steer future training for HEs. statistics, 2) unadjusted inferential statistics, 3) stratified analysis, and 4) multivariable models. My investigation produced results in accord with generally accepted notions in addition to significant findings that interestingly counter current preconceptions. Intraspecies contact was more common than inter-species, with indirect contact occurring more frequently than direct. Direct contact between species occurred extremely rarely. The most important factors that influenced the rate of contact for both species were water, winter, and cultivated fields. Information regarding probability of infectious agent survival and transfer will be used in the future to advance current epidemiological models, including geographicautomata (Ward et al. 2007: In Press) and cellular automata models (Doran and Laffan 2005) to better understand and manage integrated domestic cattle and free-ranging wildlife populations. Such modeling provides essential and necessary knowledge for developing prevention, detection, response, and recovery strategies – employed in advance, during, and after a disease outbreak, respectively.

Social perspectives on natural resources management have become an increasingly valuable part of natural resources management decision making, especially at the policy or governance level. However, due to the range of social contexts that can exist around management questions, not every technique for incorporating stakeholders into management is suited for every management problem. My research examines the social landscape around chronic wasting disease (CWD) management on the Canadian prairies in order to identify a way forward for stakeholder involvement in CWD management. CWD is a prion disease that results in neurodegeneration and death in cervids. CWD has the potential for broad social impact because it infects elk and deer, species which are both hunted and ranched. Furthermore, management and monitoring efforts in free-ranging cervids frequently incorporate hunting activity. Q methodology was used to survey stakeholders in Saskatchewan and Manitoba and synthesize perspectives about stakeholder understanding of CWD as a problem and preferences for potential solutions. The perspectives that emerged emphasized the importance of increasing knowledge about CWD and a generalized trust in government management, coupled with a desire for stakeholder consultation under the auspices of government leadership. I found that CWD management may not be ready for stakeholder spearheaded management activity due to ambivalence and uncertainty among stakeholders, but stakeholder involvement in CWD management can still offer valuable insight for managers. This is especially notable in light of the recent loss of Saskatchewan’s CWD monitoring program.