Thesis (MSc)--Stellenbosch University, 2000. / ENGLISH ABSTRACT: A digital portal X-ray imaging system was developed to replace the radiographic X-ray films currently used for patient position verification at the National Accelerator Centre (NAC) proton therapy facility. The main advantage of a digital system is the short time in which the image can be obtained. Other advantages include optimisation of the image display, effective archiving of the digital images, access from various locations through data networks, and lower operational costs. The digital system described in this thesis consists of a Gd202S:Tb scintillator screen for converting X-rays to visible light, a protected aluminum front silvered mirror to direct the light to a Charge Coupled Device (CCD) camera for capture and a personal computer for data acquisition, processing and display. Compared with other digital imaging systems, this is a simple, compact and affordable system. The properties of the various components were investigated. The Rarex G-130 (Gd202S:Tb) scintillation screen was chosen for its good spatial resolution, high emission efficiency and good matching between the spectral emission wavelength peak and the quantum efficiency of the CCD camera. The spatial resolution measured for the system with a field of view (FOV) of 290 x 190 mnr' is 1.3 lp/mm, which can be improved by increasing the CCD chip resolution or decreasing the field of view, since the CCD camera limits the spatial resolution. Intrinsic detector noise determines the lower limit of the dynamic range of the detector and is reduced by cooling the CCD camera. A dark current exposure is subtracted from the image to remove the bias signal and background signal level mainly caused by thermal noise. Photon noise, beam in-homogeneity and efficiency variations across the CCD chip are removed by a flat field correction. The digital images obtained with this system compare very well with the currently used radiographic film images and they are satisfactory for the purpose of patient position verification. Using the digital system it is possible to reduce the patient dose by 19 % and still obtain satisfactory image quality. / AFRIKAANSE OPSOMMING: 'n Digitale X-straalafbeeldingstelsel is ontwikkel om die radiografiese X-straalfilm wat tans gebruik word vir die kontrolering van die pasientposisionering voor die toediening van protonterapie by die Nasionale Versnellersentrum, te vervang. Die voordeel van die digitale sisteem is dat die beelde feitlik onmiddellik beskikbaar is. Verdere voordele sluit die optimisering van die vertoon van beeldkontras, effektiewe liassering, vinnige bereik deur datanetwerke en lae lopende kostes in. Die digitale sisteem beskryf in die tesis bestaan uit 'n gadolinium oksi-sulfied (Gd202S:Tb) sintillasieskerm wat X-strale omskakel na sigbare lig, 'n eerste-oppervlak aluminiumspieël wat die lig na 'n digitale kamera (CCD kamera) weerkaats en In persoonlike rekenaar vir dataverwerwing, verwerking en vertoon. Vergeleke met ander digitale stelsels is hierdie digitale beeldingstelsel eenvoudig, kompak en bekostigbaar. Die eienskappe van die verskillende komponente van die stelsel is ondersoek. Die Rarex G- 130 (Gd202S:Tb) sintillasieskerm IS gekies vanweë goeie resolusie, hoë emissiedoeltreffendheid en die hoë omsettingsdoeltreffendheid van die digitale kamera by die spektrale emissiegolflengte van dié sintillasieskerm. Die ruimtelike oplosvermoë van die stelsel is bepaal met In veldgrootte van 290 x 190 mnr' as 1.3 lynpare per millimeter. Die ruimtelike oplosvermoë kan verhoog word deur die kameraresolusie te verhoog of die veldgrootte te verklein, omdat die resolusie van die kamera tans die oplosvermoë van die stelsel beperk. Intrinsieke ruis van die detektor beperk die onderste grens van die dinamiese reikwydte van die detektor en kan verminder word deur die kamera te verkoel. 'n Donkerstroom-beeld word van die X-straalbeelde afgetrek om die voorspanningsein en die agtergrondsein, wat hoofsaaklik veroorsaak word deur termiese ruis, te verwyder. Ruis wat ontstaan as gevolg van fluktuasies in die aantal fotone, nie-homogeniteite in die bundel of variasie van die sensitiwiteit in die skerm word verwyder met behulp van 'n plat vlak beeld. Die digitale beelde verkry met die stelsel vergelyk goed met die beelde wat tans op film geneem word en die beeldkwalitiet is voldoende vir die kontrolering van die pasientopstelling. Dit is moontlik om die pasiëntdosis met 19 % te verminder en steeds voldoende beeldkwaliteit te verkry.

Structures were determined for 48 new natural products and several related compounds by NMR methods. One new natural product and two unnatural product structures were determined by X-ray diffraction. Molecular mechanics calculations on two indoles related to the neurotransmitter serotonin and on some synthetic cyclophanes were used to gain information about their preferred conformations. Considerable time is wasted redetermining the structures of known natural products when they are encountered in new sources. To help alleviate this problem, a database which searches on proton NMR chemical shifts was developed.

General relativity has been tested by many experiments, which, however, almost exclusively probe weak spacetime curvatures. In this thesis, I create two frameworks for testing general relativity in the strong-field regime with observations of black holes in the electromagnetic spectrum using current or near-future instruments. In the first part, I design tests of the no-hair theorem, which uniquely characterizes the nature of black holes in terms of their masses and spins in general relativity and which states that these compact objects are described by the Kerr metric. I investigate a quasi-Kerr metric and construct a Kerr-like spacetime, both of which contain an independent parameter in addition to mass and spin. If the no-hair theorem is correct, then any deviation from the Kerr metric has to be zero. I show that already moderate changes of the deviation parameters in either metric lead to significant modifications of the observed signals. First, I apply this framework to the imaging of supermassive black holes using very-long baseline interferometry. I show that the shadow of a black hole as well as the shape of a bright and narrow ring surrounding the shadow depend uniquely on its mass, spin, inclination, and the deviation parameter. I argue that the no-hair theorem can be tested with observations of the supermassive black hole Sgr A*. Second, I investigate the potential of quasi-periodic variability observed in both galactic black holes and active galactic nuclei to test the no-hair theorem in two different scenarios. Third, I show that the profiles of relativistically broadened iron lines emitted from the accretion disks of black holes imprint the signatures of deviations from the Kerr metric. In the second part, I devise a method to test the predicted evaporation of black holes in the Randall-Sundrum model of string theory-inspired braneworld gravity through the orbital evolution of black-hole X-ray binaries and obtain constraints on the size of the extra dimension from A0620-00 and XTE J1118+480. I predict the first detection of orbital evolution in a black-hole binary.

This thesis focuses on how physical and biological factors influence the outcome of exposures to γ/X-rays. That the dose rate changes during real life exposure scenarios is well-known, but radiobiological data from exposures performed at increasing or decreasing dose rates is lacking. In paper I, it was found that an exposure where the dose rate decreases exponentially induces significantly higher levels of micronuclei in TK6 cells than exposures at an increasing or constant dose rate. Paper II describes the construction and validation of novel exposure equipment used to further study this “decreasing dose rate effect”, which is described in paper III. In paper I we also observed a radioprotective effect when cells were exposed on ice. This “temperature effect” (TE) has been known for decades but it is still not fully understood how hypothermia acts in a radioprotective manner. This was investigated in paper IV, where a multiparametric approach was used to investigate the underlying mechanisms. In paper V the aim was to investigate the role of biomarkers and clinical parameters as possible risk factors for late adverse effects to radiotherapy (RT). This was studied in a rare cohort of head-and-neck cancer patients that developed mandibular osteoradionecrosis (ORN) as a severe late adverse effect of RT. Biomarker measurements and clinical factors were then subjected to multivariate analysis in order to identify ORN risk factors. The results suggest that the patient’s oxidative stress response is an important factor in ORN pathogenesis, and support the current view that patient-related factors constitute the largest source of variation seen in the frequency of late adverse effects to RT. In summary, this thesis provides new and important insights into the roles of biological and physical factors in determining the consequences of γ/X-ray exposures. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: Submitted. Paper 5: Manuscript.</p>

In this thesis I use X-ray observations to study the cores and extended structures of radio-loud AGN, to determine their structure, accretion properties and the impact they have on their surroundings. I use new Chandra data and archival XMM-Newton observations ofMarkarian 6 to look for evidence of emission from shocked gas around the external radio bubbles, using spatially resolved regions in Chandra and spectral analysis of the XMM data. The results show that the bubbles in Mrk 6 are indeed driving a shock into the halo of the host galaxy, with a Mach number of 3.9. I also find that the spectrum of the AGN has a variable absorbing column, which changes from 8 × 1021 atoms cm−2 to 3 × 1023 atoms cm−2 on short timescales (2-6 years). This is probably caused by a clump of gas close to the central AGN, passing in front of us at the moment of the observation. Using new and archival Chandra observations of the Circinus galaxy, I match them to pre-existing radio, infrared and optical data to study the kpc-scale emission. As for Mrk 6, I find that the radio bubbles in Circinus are driving a shock into the interstellar medium of the host galaxy, with Mach numbers M 2.7–3.6 and M 2.8–5.3 for the W and E shells respectively. Comparing the results with those we previously obtained for Centaurus A, NGC 3801 and Mrk 6, I show that the total energy in the lobes (thermal+kinetic) scales approximately with the radio power of the parent AGN. The spatial coincidence between the X-ray and edge-brightened radio emission in Circinus resembles the morphology of some SNR shocks, a parallel that has been expected for AGN, but has never been observed before. I investigate what underlying mechanisms both types of systems may have in common, arguing that, in Circinus, the edge-brightening in the shells may be accounted for by a B field enhancement caused by shock compression, but do not preclude some local particle acceleration. I also carry out a systematic study of the X-ray emission from the cores in the 0.02 < z < 0.7 2Jy sample, using Chandra and XMM-Newton observations. I combine the results with the mid-IR, optical emission line and radio luminosities, and compare them with those of the 3CRR sources, to show that the low-excitation objects in our sample show all the signs of radiatively inefficient accretion. I study the effect of the jet-related emission on the various luminosities, confirming that it is the main source of soft X-ray emission for our sources. I also find strong correlations between the accretion-related luminosities, and identify several sources whose optical classification is incompatible with their accretion properties. I derive the bolometric and jet kinetic luminosities for the sample and find a difference in the total Eddington rate between the low and high-excitation populations, with the former peaking at 1 per cent and the latter at 20 per cent Eddington. There is, however, an overlap between the two, indicating that a simple Eddington switch may not be possible. The apparent independence of jet kinetic power and radiative luminosity in the highexcitation population in our plots allows us to test the hypothesis in which jet production and radiatively efficient accretion are in fact independent processes that can coexist in high-excitation objects.

We report the identification of a bright hard X-ray source dominating the M31 bulge above 25 keV from a simultaneous NuSTAR-Swift observation. We find that this source is the counterpart to Swift J0042.6+4112, which was previously detected in the Swift BAT All-sky Hard X-ray Survey. This Swift BAT source had been suggested to be the combined emission from a number of point sources; our new observations have identified a single X-ray source from 0.5 to 50 keV as the counterpart for the first time. In the 0.5-10 keV band, the source had been classified as an X-ray Binary candidate in various Chandra and XMM-Newton studies; however, since it was not clearly associated with Swift J0042.6+4112, the previous E < 10 keV observations did not generate much attention. This source has a spectrum with a soft X-ray excess (kT similar to 0.2 keV) plus a hard spectrum with a power law of Gamma similar to 1 and a cutoff around 15-20 keV, typical of the spectral characteristics of accreting pulsars. Unfortunately, any potential pulsation was undetected in the NuSTAR data, possibly due to insufficient photon statistics. The existing deep HST images exclude high-mass (> 3 M-circle dot) donors at the location of this source. The best interpretation for the nature of this source is an X-ray pulsar with an intermediate-mass (< 3 M-circle dot) companion or a symbiotic X-ray binary. We discuss other possibilities in more detail.

We present multi-epoch mid-infrared (IR) photometry and the optical discovery observations of the "impostor" supernova (SN) 2010da in NGC. 300 using new and archival Spitzer Space Telescope images and ground-based observatories. The mid-infrared counterpart of SN. 2010da was detected as Spitzer Infrared Intensive Transient Survey (SPIRITS). 14bme in the SPIRITS, an ongoing systematic search for IR transients. Before erupting on 2010 May 24, the SN. 2010da progenitor exhibited a constant mid-IR flux at 3.6 and only a slight similar to 10% decrease at 4.5 mu m between 2003 November and 2007 December. A sharp increase in the 3.6 mu m flux followed by a rapid decrease measured similar to 150 days before and similar to 80 days after the initial outburst, respectively, reveal a mid-IR counterpart to the coincident optical and high luminosity X-ray outbursts. At late times, after the outburst (similar to 2000 days), the 3.6 and 4.5 mu m emission increased to over a factor of two. times the progenitor flux and is currently observed (as of 2016 Feb) to be fading, but still above the progenitor flux. We attribute the re-brightening mid-IR emission to continued dust production and increasing luminosity of the surviving system associated with SN. 2010da. We analyze the evolution of the dust temperature (T-d similar to 700-1000 K), mass (Md similar to 0.5-3.8 x. 10(-7) M circle dot), luminosity (L-IR similar to 1.3-3.5 x 10(4) L circle dot), and the equilibrium temperature radius (R-eq similar to 6.4-12.2 au) in order to resolve the nature of SN. 2010da. We address the leading interpretation of SN. 2010da as an eruption from a luminous blue variable high-mass X-ray binary (HMXB) system. We propose that SN. 2010da is instead a supergiant (sg)B[e]-HMXB based on similar luminosities and dust masses exhibited by two other known sgB[e]-HMXB systems. Additionally, the SN. 2010da progenitor occupies a similar region on a mid-IR color-magnitude diagram (CMD) with known sgB[e] stars in the Large Magellanic Cloud. The lower limit estimated for the orbital eccentricity of the sgB[e]-HMXB (e > 0.82) from X-ray luminosity measurements is high compared to known sgHMXBs and supports the claim that SN. 2010da may be associated with a newly formed HMXB system.

The charge state dependence of M-shell x-ray production cross sections of 67HO bombarded by 2-12 MeV carbon ions with and without K-vacancies are reported. The experiment was performed using an NEC 9SDH-2 tandem accelerator at the Ion Beam Modification and Analysis Laboratory of the University of North Texas. The high charge state carbon ions were produced by a post-accelerator stripping gas cell. Ultra-clean holmium targets were used in ion-atom collision to generate M-shell x rays at energies from 1.05 to 1.58 keV. The x-ray measurements were made with a windowless Si(Li) x-ray detector that was calibrated using radiative sources, particle induced x-ray emission (PIXE), and the atomic field bremsstrahlung (AFB) techniques.

Um injetor de pastilhas (pellets) de impurezas foi projetado, construído e instalado junto ao tokamak TCABR do Instituto de Física da Universidade de São Paulo - USP. O injetor é basicamente constituído por uma câmara com gás a alta pressão cuja vazão é controlada por uma bobina de acionamento rápido. Uma fonte de alta tensão (7kV) e alta corrente (6kA) foi construída para alimentar a bobina. Ao ser disparada, o gás propulsor (N2 ou He) acelera a pastilha para o interior do vaso do tokamak, sem que gás penetre no mesmo. Pastilhas de grafite cilíndricas com diâmetro de 0,4mm a 0,9mm, e comprimento de 0,5mm a 1mm, foram utilizadas para investigar a possibilidade de se mitigar os e*feitos de uma disrupção maior O processo de ablação da pastilha no TCABR foi investigado através de simulações que consideraram, de uma maneira simplificada, o resfriamento sofrido pelo plasma devido à propagação da pastilha no seu interior. O modelo, ao ser aplicado aos dados experimentais do tokamak T-10, por exemplo, apresentou resultados bastante encorajadores. Na maioria dos casos em que houve a injeção das pastilhas no TCABR, o plasma terminou devido ao surgimento de uma disrupção maior. Analisando a taxa de queda da corrente de plasma em dois instantes próximos ao fim da descarga, tanto para pulsos que sofreram uma disrupção natural quanto aos que sofreram um disrupção induzida (devido à injeção de pastilhas de grafite), constatou-se que a corrente de plasma decresce mais lentamente nos casos em que houve o disparo do injetor. Isto significa uma menor sobrecarga nos sistemas eletro-mecânicos do tokamak, em resultado à ocorrência da disrupção. A análise da atividade MHD foi realizada no entorno da disrupção causada pela injeção de pastilha. Observou-se, em conseqüência, uma redução da velocidade de rotação das ilhas magnéticas após o início da interação da pastilha com o plasma. Também constatou-se, em todos os disparos analisados, um aumento significativo da atividade MHD. Um outro resultado interessante foi a constatação de que a emissão de raios-X de altas energias decresce significativamente no final das descargas nas quais ocorre a injeção de pastilhas. / An impurity pellet injector has been projected, built and installed in the TCABR tokamak, at the Physics Institute of Physics of the University of São Paulo - USP. Basically, the injector is composed of a high pressure gas chamber, in which the gas flow (N2 or He) is controlled by a fast switch valve. An high voltage (7kA) and high electric current (6kA) power supply has been built to provide energy for the valve. When fired, the propellant gas move the pellet into the interior of the tokamak vessel. During this process, the gas is properly collected before reaching the tokamak vacuum chamber. For this work, cylindrical carbon pellets with 0,4mm to 0,9mm in diameter and 0,5mm to 1mm in length were chosen as to investigate how the hazardous effects of a major disruption could be mitigated. The pellet ablation process in TCABR was studied trough running simulations that take into account, in a simplified way, the cooling of the plasma by the propagating pellet. The model, when applied to the T-10 tokamak experimental data, for example, exhibited very encouraging results. For the TCABR tokamak, in most of the cases in which pellets were injected, the plasma was terminated because of the advent of a major disruption. By analyzing the plasma current decay rate in two time intervals - within the end of plasma discharges, with and without the injection of pellets, it was observed that the plasma current decays significantly slower when pellets are injected. Consequently the load on the tokamak\'s electromechanical systems is reduced. Fourier analysis has been carried out to investigate the MHD activity near the disruption time, caused by the pellet injection. It could be noticed a reduction on the magnetic island\'s velocity rotation, after the pellet-plasma interaction initiates. Also, for all discharges analyzed, the MHD activities increased in amplitude after the pellet-plasma interaction. Another interesting result refers to the fact that the hard X-ray emission was observed to decrease significantly within the end of discharges in which pellets were injected

O conhecimento preciso do espectro energético de fótons é de extrema importância na radioterapia no que se refere à escolha adequada das doses a que os pacientes são submetidos. Os dados de saída dos detectores de radiação são apenas espectros de altura de pulso (PHD), ao invés de espectros de energia, que correspondem a informações distorcidas sobre a fonte e seus decaimentos devido a diversos erros associados ao processo de cintilação do cristal e à eletrônica. Os resultados medidos foram obtidos com o uso de um detector cintilador de NaI(Tl) e as simulações Monte Carlo foram feitas com o uso do programa EGSnrc. A partir das características do detector obtidas experimentalmente, nossas simulações puderam ser validadas para se aproximarem o mais próximo possível do caso real em laboratório. Dessas medidas experimentais de fontes radioativas de calibração e simulações, foi montada uma matriz resposta inversa que transforma PHD em espectro de energia de fótons. Uma vez que a faixa energética das fontes radioativas medidas teve um limite de 1.6MeV, a matriz resposta também o apresenta, mas que pode ser contornado de acordo com a geometria usada para a obtenção do PHD a ser corrigido. Observa-se que a resposta do detector é diretamente proporcional à energia e assim, dependendo da energia incidente a ser estudada, dois tipos de matriz resposta inversa podem ser aplicadas. Os resultados desta transformação de PHD para espectro de energia de fótons são apresentados. / The accurate knowledge of the photon energy spectrum in radiotherapy is of extremely importance when it comes to the appropriate choice for doses whose patients are submitted. The output data from the radiation detectors is described as pulse high distributions, instead of energy spectra, that correspond to distorted information about the source and its decays due to many errors associated to the crystal scintillation process and the electronics. The measured results were obtained with a NaI(Tl) scintillation detector and Monte Carlo simulations were performed by the EGSnrc program. From the detector characteristics obtained experimentally, the simulations were validated so they could approximate the more real as possible as in laboratory. Using these simulated and experimental calibration sources, an inverse response matrix was built and transforms pulse high distributions in photons energy spectrum. Once the correction energy limit due to the radiation sources used is approximately 1.6 MeV, so the response matrix has a limit, but it can be overcame by the beam incident angle and the geometry used when the pulse high distributions to be corrected is going to be measured. One can realize that the detector response is directly proportional to the incident energy and, when it is intense as in a LINAC or weak as in a calibration source, different types of matrix can be applied. The results from these transformations are presented.