The first aim of this study is the development of extracellular recombinant therapeutic protease streptokinase producing Bacillus sp., and the second aim is to determine fermentation characteristics for streptokinase production. In this context, the signal (pre-) DNA sequence of B.licheniformis (DSM1969) extracellular serine alkaline protease enzyme gene (subC: Acc. No. X03341) was ligated to 5&rsquo / end of the streptokinase gene (skc: Acc. No. S46536) by SOE (Gene Splicing by Overlap Extension) method through PCR. The resulting hybrid gene pre(subC)::skc was cloned into the pUC19 plasmid. Then, the hybrid gene was sub-cloned to pMK4 plasmid which is an E. coli-Bacillus shuttle vector with high copy number and high stability. Recombinant plasmid pMK4::pre(subC)::skc was finally transferred into B. subtilis (npr- apr-) and B. licheniformis 749/C (ATCC 25972) species. Streptokinase production capacities of these two recombinant Bacillus species were compared. The highest production was observed in recombinant B. lichenifomis 749/C (ATCC 25972) strain in a defined medium which was optimized in terms of carbon and nitrogen sources by a statistical approach, namely Response Surface Methodology (RSM). RSM evaluated the streptokinase concentration as the response and the medium components as the independent variables. The highest recombinant streptokinase concentration was found as 0.0237 kgm-3 at glucose and (NH4)2HPO4 concentrations of 4.530 and 4.838 kgm-3 respectively. The fermentation and oxygen transfer characteristics of the streptokinase production were investigated in a 3 dm3 pilot scale batch bioreactor (Braun CT2-2) equipped with temperature, pH, foam, air inlet and agitation rate controls having a working volume of VR=1.65 dm3 using the production medium optimized for the recombinant B. lichenifomis 749/C (ATCC 25972) strain. Streptokinase and &amp / #946 / -lactamase activities, cell, glucose and organic acid concentrations, dissolved oxygen, pH, oxygen uptake rate, overall liquid phase mass transfer coefficient for oxygen, maintenance coefficient for oxygen, specific cell growth rate and yield coefficients were determined through the bioprocess. The bioprocess of recombinant streptokinase production was performed at uncontrolled pH of these bioreactor operation conditions: air inlet rate of Q0/VR=0.5 vvm, and the agitation rate of N=400min-1. The resulting streptokinase volumetric activity reached its maximum as 1.16 PUml-1 (0.0026 g/l streptokinase) at t=20 h.

The main objective of this study is to model and analyze a three dimensional inclined semi-elliptic surface crack in a Functionally Graded Material (FGM) coating bonded to a homogeneous substrate with a bond coat. The parametric analyses on FGMs are based upon zirconia-yttria (ZrO2-8wt%-Y2O3) FGM coating bonded to a substrate made of a nickel-based superalloy. It is assumed that there is a nickel-chromium&amp / #8211 / aluminum&amp / #8211 / zirconium (NiCrAlY) bond coat between the FGM coating and substrate. Metal-rich, linear variation, ceramic-rich and homogeneous ceramic FGM coating types are considered in the analyses. The inclined semi-elliptic surface crack problem in the FGM coating-bond coat-substrate system is analyzed under transient thermal loading. This problem is modeled and analyzed by utilizing three dimensional finite elements. Strain singularity around the crack front is simulated using collapsed 20 &amp / #8211 / node quarter &amp / #8211 / point brick elements. Three &amp / #8211 / dimensional displacement correlation technique is utilized to extract the mixed mode stress intensity factors around the crack front for different inclination angles of the semi-elliptic surface crack. The energy release rates around the crack front are also calculated by using the evaluated mixed mode stress intensity factors. The results obtained in this study are the peak values of mixed mode stress intensity factors and energy release rates around the crack front for various inclination angles of the semi-elliptic surface crack embedded in the FGM coating of the composite structure subjected to transient thermal loading.

The main aim of this study was to investigate the secondary schools English teachers&amp / #8217 / perceptions of technology, technology integration processes into their lessons, and the ways they use technology for professional development. The secondary aim of the study was to propose technology integration guidelines to enable high school English teachers to integrate technology into their teaching. Qualitative research design was used in this study and it resembles multi-case studies. For the participants&amp / #8217 / selection, criterion and convenience sampling strategy was used. First, 17 high schools (4 private, 3 Anatolian, 6 regular, and 4 vocational) were determined in Ankara province, and then 17 English teachers, one from each school, were selected based on the predetermined criteria. Totally, 17 teachers and 17 administrators were included in the study. Observations, document analysis, and interviews were used to collect the data. The data were analyzed through content analysis. The data were categorized under emerged themes, general technology knowledge, planning, using, evaluation and assessment, personal purposes, attitudes, support, and wishes. The findings of the study indicated that private high school teachers perceived themselves more knowledgeable in technology knowledge than regular, Anatolian, and vocational high school teachers. In addition, the interview results showed that private high school teachers integrate technology into planning, instruction, evaluation and assessment, and professional development more than the other English teachers. When school resources and support mechanisms were compared, private high schools were in a better condition than public high schools. Finally, most of the administrators included in the study wanted teachers use available school resources in their lessons.

This thesis presents a new developed method for first intermolecular aldehyde/ketone cross benzoin coupling. Protected &amp / #945 / -keto tertiary alcohols are synthesized starting from easily available acyl phosphonates and ketones via Brook rearrangement in the presence of catalytic amount of cyanide ion. The scopes and the limitations of the methods for the synthesis of tertiary alcohols with &amp / #945 / -keto group are discovered.

In this study, three aspects of technology integration in English Language Teaching within the context of private universities in Ankara, Turkey were investigated. Firstly, preservice and in-service teachers&amp / #8217 / computer usage frequencies/types, computer competence levels, perceived barriers to technology integration and attitudes toward computers were explored. Then, factors (age, gender, work experience, institutional factors being preservice or in-service) that might potentially affect the findings of the first research question were examined. Finally, the educational value preservice and in-service teachers assigned to technology usage in their language teaching practices and their ideas on effective technology integration were scrutinized. In order to reach aforementioned goals, both qualitative and quantitative data were collected with the help of a questionnaire and semi-structured face-to-face interviews. The institutions sampled in this study were all private universities, the infrastructure of which varied drastically. A total of 182 questionnaires collected from the teachers (in-service N=120, preservice N=62), as well as eight in-service and four preservice teachers were interviewed. The findings indicated that teachers used computers at their schools at limited frequency. It was also found that they indicated high levels of instructional computer usage outside the school and technology competence. It was also seen that age, gender and the institutions the teachers worked at affected their technology usage and competence levels. As for the educational value assigned to technology usage in their language teaching, the teachers indicated that technology would help make their lessons more student centered. However, the teachers mainly mentioned using technology as teacher tools rather student tools which help foster higher order thinking skills and learner autonomy. Keeping the usage statistics in mind, it was concluded that the schools, even though all of them were private, lacked computer infrastructure to the point that the teachers had difficulty even to use computers for their personal purposes. It was also concluded that the schools in Turkey were still at the stage of fighting with first-order barriers, even at private institutions, indicating that a vision towards technology integration lacks. As for the educational value assigned to computer usage in ELT, it was concluded that institutional barriers were more of a concern for the teachers as they did not have a chance to delve into actual instructional usage and the ICT courses at college were not preparing the teachers for effective technology integration due to lack of proper training activities.

Research by design refers to the design of architectural research as an integral part of architectural design processes. In 1980s, it emerged as a third way in design research that was dominated until then by the methods of natural sciences and humanities. With this new formulation of design research, a methodological and epistemological transformation occurs, leading to the integration of practical knowledge into architectural research. The primary epistemological question transforms from knowing what design is and knowing how to design to knowing what through the act of design. The integration of the act of design in research transforms the status of design in design research from being an object of inquiry to being a research approach. In the literature on research by design, this transformation is often related with Donald Sch&ouml / n&rsquo / s conceptualization of &ldquo / reflective practice.&rdquo / The main discussion of reflective practice is primarily methodological rather than epistemological. Although it provides methodological insights, it is not sufficient to constitute an epistemological basis for research by design. Thus, the epistemological basis of research by design has not yet been adequately defined. In this study, the notion of &ldquo / reflective practice&rdquo / is investigated in a broader context relating it to its sources in the concepts of &ldquo / tacit knowledge&rdquo / and &ldquo / action research.&rdquo / A conceptual framework for research by design is constructed by relating these concepts with the discussions on research by design and with practical philosophy, the implications of which has remained rather uninvestigated in this context. Aristotle&rsquo / s elaboration of knowledge generation in action and the concept of phron&amp / #275 / sis (practical knowledge, prudence, or practical wisdom) constitute the underpinning of this conceptual framework. The conceptual framework that is constructed on the basis of the key concepts in practical philosophy is discussed in the context of architectural design education. When architectural design education is formulated as a process of research by design within this framework, knowledge generated in the educational design processes promises not only to improve the particular educational context and architectural education but eventually to contribute to architectural knowledge.

This research aims to analyze the international political economy of rising China since the mid 1990s. The main question it tries to answer / why in the early 21st century, Chinese officials defined China&amp / #8217 / s position within the international system as Peaceful Rise in theory, in rhetoric and in policy. The research studies the question based on analysis of international political economy of China&amp / #8217 / s reform process within a historical perspective. Given China&amp / #8217 / s history-long &amp / #8216 / &amp / #8216 / catching up with the West&amp / #8217 / &amp / #8217 / as the main drive behind the determination and the guidance of China&amp / #8217 / s strategy at home and abroad throughout the political history of modern China, the thesis argues that &amp / #8216 / &amp / #8216 / China&amp / #8217 / s Rise&amp / #8217 / &amp / #8217 / has became the new strategy of China&amp / #8217 / s catching up objective towards the 21st century. Chinese leadership formulated the concept of Peaceful Rise as the discourse of the new policy to both domestic and external audiences. Building up Harmonious Society and being a Soft Power in international order have been designed as the policy components of new strategy. Chinese leadership also used the concept of Peaceful Rise as the theory of the legitimization of the new strategy of &amp / #8216 / &amp / #8216 / China&amp / #8217 / s Rise&amp / #8217 / &amp / #8217 / and its policy components.

The synthesis of cyclic allenes with eight or less skeletal C-atoms, known as highly strained organic compounds, has for the past decades attracted increasing interest. The first part of study describes an investigation aimed at the incorporation of an allene unit into a natural compound, being &amp / #945 / -pinene, by using &amp / #946 / -elimination method. The two double-bond isomers 310 and 299b were synthesized as key compounds. Treatment of 310 with t-BuOK resulted in the formation of ketone 308 and diene 313. For the formation of 308, the cyclic allene 300 was proposed as an intermediate. Treatment of 299b, with t-BuOK gave arise to the diene 313 and the dimerization product 322. On the basis of density-functional-theory (DFT) calculations on the allene 300 and the alkyne 320, the formation of the latter as the intermediate was excluded. In the second part of study, the stability of endo-carbene 304 was investigated. Previous studies indicated, during the formation of intermediate 264, no exo-carbene 330 structure could be optimized in its free carbene form. At this point, we were curious about the stability of endo-cyclopropylidene 304 not discussed before in literature. First, addition of bromofluorocarbene to anti-7-ethylbenzonorbornadiene (352) was aimed to isolate the endo-adduct 302b. However, no carbene addition reaction was observed caused by pyramidalization on double bond respect to the methoxy derivative, 363b. Therefore, the bromine was introduced to C-7 carbon atom. Treatment of 302a with MeLi in the presence of furan, gave furan adduct 306a confirmed the formation of allene 305a as a reactive intermediate. Theoretical calculations showed endo-carbene 304a was optimized in the free carbene form . However, it readily isomerizes to allene 305a afforded furan adduct 306a.

Ischemic brain injury causes neurodegeneration. In this study, the mechanism of neurodegeneration was investigated by examining the role of sterol regulatory element binding protein-1 (SREBP-1), CCAAT enhancer binding protein&amp / #946 / (C/EBP&amp / #946 / ), glutathione (GSH), malondialdehyde (MDA), glutathione-S-transferase (GST), and superoxide dismutase (SOD). Carotid artery occlusion (CAO) plus hypotension was produced for 10 minutes. Control groups were sham operated. Animals were sacrificed after 24 hours, 1 week, 2 and 4 weeks of reperfusion periods. The expression of C/EBP&amp / #946 / and SREBP-1 in rat brain cortex and cerebellum were examined by western blotting. C/EBP&amp / #946 / expressions significantly increased in both cytosolic (1.19, 1.58 fold) and nuclear (1.73, 1.81 fold) extracts of brain cortex at 24 hours and 1 week CAO groups, respectively. In cerebellum, C/EBP&amp / #946 / expression significantly increased in 1 week, cytosolic (1.63 fold), and nuclear (1.35 fold) extracts. SREBP-1 expression increased significantly in both cytosolic (2.07 fold) and nuclear (1.41 fold) extracts of brain cortex in 1 week. SREBP-1 expression significantly increased in cytosolic (2.15 fold) and nuclear (1.79 fold) extracts of cerebellum in 1 week. There were no significant alterations in SREBP-1 C/EBP&amp / #946 / expressions for 2 and 4 weeks in both cytosolic and nuclear extracts of brain cortex and cerebellum. There were insignificant changes in GSH and GST levels in cortex. However, MDA and SOD levels significantly increased by 43.0 % and 47.3 %, respectively, in 24 hours. Our findings indicate that increase in SREBP-1 and C/EBP&amp / #946 / expressions may be related to oxidative stress during ischemic neurodegenerative processes.

The use of various biodegradable polymers for the improvement of different controlled and long-lasting drug release systems is an active research area in recent years. The application of different metal prostheses, especially titanium based ones, to the human body is also very common. A most important disadvantage of these prostheses is the risk of infection at the application areas that necessitates the removing of the prosthesis with a second surgical operation and reapplication of it after recovery. One of the best ways to solve this problem is to render metal prostheses infection free with controlled and sustainable drug (antibiotic) release systems. The long term sustained release of relevant antibiotics from the various biodegradable polymer coated metal implants is studied in this thesis. Virtual fatigue analysis and drug loading capacities of titanium and stainless steel samples with different surface pattern and modifications were studied. Various biodegradable polymer and drug combinations were examined and used for coating of metal prosthesis. The aim is to design polymer-drug coated metal implants that are capable of releasing a feasible amount of drug up to a period of at least 1 month. Various coating techniques and surface modifications were also employed to improve the adhesional properties of the drug containing polymers. Their adhesion abilities on the metal substrates were tested by Lap-shear and T-peel tests. Polymer degradation kinetics was followed by viscosity studies. Calibration lines for different drugs were obtained and drug releases on different systems were followed by using UV spectroscopy and microbial antibiotic sensitivity tests. Among the techniques applied to prevent fast release of drugs initially, the coatings of Vancomycin absorbed &amp / #946 / -TCP (&amp / #946 / -tricalcium phosphate) homogeneously distributed in poly(D,L-lactide-co-glycolide) solution in chloroform followed by an inert coating with poly(L-lactide) system proved to be feasible. By this technique, initial burst release was minimized and drug release from implants lasted nearly 2 months. Multiple coatings on polymer plus drug coating layer also gave promising results. In vivo studies on dorsal muscles of native rabbits with antibiotic loaded implants gave no negative effect on the surrounding tissues with high compatibility free of infection.