Nitrogenase has a central role in the global nitrogen cycle as the enzyme that catalyzes the reduction of atmospheric N2 to NH3. Fixed nitrogen is generally limiting in the environment and in agriculture, so nitrogenase has received much attention as an alternative to nitrogen fertilizers. Characterizing the mechanism of nitrogenase is the goal of this work. The molybdenum nitrogenase enzyme system is comprised of the MoFe protein and the Fe protein. Interactions between these proteins and nucleotides are crucial to catalysis. An important approach to characterize these interactions is to correlate the kinetics of nitrogenase catalysis to a mechanism based on the properties of the nitrogenase components. Ironically, the most successful kinetic model of nitrogenase was devised by R. N. F. Thorneley and D. J. Lowe (T&L) before any crystal structures of nitrogenase were solved. This work critiques the ability of the T&L model to predict nitrogenase catalysis accurately. Several defects in the model are described, but it is qualitatively correct. A literature review and critique leads to the rational design of a new kinetic model of nitrogenase catalysis. Because of its comprehensive scope and superior detail, this model has the potential to describe nitrogenase catalysis quantitatively. However, the development of this model is an ambitious project only begun in this work, step by step. Some of the areas of study include: an analysis of Fe protein reduction by dithionite; the characterization of a form of Fe protein reduced to the all-ferrous [4Fe-4S]0 state with a novel spin S = 0 state by the in vivo reductant flavodoxin; and a novel account of salt effects that weaken the nitrogenase complex to increase the rate of complex dissociation, the rate-limiting step in nitrogenase catalysis.

All-optical synchronization and its application in advanced optical communications have been investigated in this dissertation. Dynamics of all-optical timing synchronization (clock recovery) using multi-section gain-coupled distributed-feedback (MS-GC DFB) lasers are discussed. A record speed of 180-GHz timing synchronization has been demonstrated using this device. An all-optical carrier synchronization (phase and polarization recovery) scheme from PSK (phase shift keying) data is proposed and demonstrated for the first time. As an application of all-optical synchronization, the characterization of advanced modulation formats using a linear optical sampling technique was studied. The full characterization of 10-Gb/s RZ-BPSK (return-to-zero binary PSK) data has been demonstrated. Fast lockup and walk-off of the all-optical timing synchronization process on the order of nanoseconds were measured in both simulation and experiment. Phase stability of the recovered clock from a pseudo-random bit sequence signal can be achieved by limiting the detuning between the frequency of free-running self-pulsation and the input bit rate. The simulation results show that all-optical clock recovery using TS-DFB lasers can maintain a better than 5 % clock phase stability for large variations in power, bit rate and optical carrier frequency of the input data and therefore is suitable for applications in ultrafast optical packet switching. All-optical timing synchronization of 180-Gb/s data streams has been demonstrated using a MS-GC DFB laser. The recovered clock has a jitter of less than 410 fs over a dynamic range of 7 dB. All-optical carrier synchronization from phase modulated data utilizes a phase sensitive oscillator (PSO), which used a phase sensitive amplifier (PSA) as a gain block. Furthermore, all-optical carrier synchronization from 10-Gb/s BPSK data was demonstrated in experiment. The PSA is configured as a nonlinear optical loop mirror (NOLM). A discrete linear system analysis was carried out to understand the stability of the PSO. Complex envelope measurement using coherent linear optical sampling with mode-locked sources is investigated. It is shown that reliable measurement of the phase requires that one of the optical modes of the sampling pulses be locked to the optical carrier of the data signal to be measured. Carrier-envelope offset (CEO) is found to have a negligible effect on the measurement. Measurement errors of the intensity profile and phase depend on the pulsewidth and chirp of the sampling pulses as well as the detuning between the carrier frequencies of the data signal and the center frequency of the sampling source. Characterization of the 10-Gb/s RZ-BPSK signal was demonstrated using the coherent detection technique. Measurements of the optical intensity profile, chirp and constellation diagram were demonstrated. A CW local oscillator was used and electrical sampling was performed using a sampling scope. A novel feedback scheme was used to stabilize homodyne detection.

"The primary purpose of this study was to identify health education strategies which are effective in increasing the use of protective devices which can prevent injury. The specific protective device studied was the helmet worn by off-road vehicle [including All-Terrain Vehicles [ATVs] and snowmachines] riders in rural Alaska"--Leaf 3. / Thesis (Ed. D.)--Boston University, 1996.

The structure and dynamics of membranes are crucial to ensure the proper functioning of cells. There are some compounds used in therapeutics that show nonspecific interactions with membranes in addition to their specific molecular target. Among them, two compounds recently used in therapeutics against COVID-19, remdesivir and favipiravir, were subjected to molecular dynamics simulation assays. In these, we demonstrated that the compounds can spontaneously bind to model lipid membranes in the presence or absence of cholesterol. These findings correlate with the corresponding experimental results recently reported by our group. In conclusion, insertion of the compounds into the membrane is observed, with a mean position close to the phospholipid head groups.

All-polymer solar cells (APSC) are a class of organic solar cells in which hole and electron transporting phases are made of conjugated polymers. Unlike polymer/fullerene solar cell, photoactive material of APSC can be designed to have hole and electron transporting polymers with complementary absorption range and proper frontier energy level offset. However, the highest reported PCE of APSC is 5 times less than that of polymer/fullerene solar cell. The low PCE of APSC is mainly due to: i) low charge separation efficiency; and ii) lack of optimal morphology to facilitate charge transfer and transport; and iii) lack of control over the exciton and charge transport in each phase. My research work is focused towards addressing these issues. The charge separation efficiency of APSC can be enhanced by designing novel electron transporting polymers with: i) broad absorption range; ii) high electron mobility; and iii) high dielectric constant. In addition to with the above parameters chemical and electronic structure of the repeating unit of conjugated polymer also plays a role in charge separation efficiency. So far only three classes of electron transporting polymers, CN substituted PPV, 2,1,3-benzothiadiazole derived polymers and rylene diimide derived polymers, are used in APSC. Thus to enhance the charge separation efficiency new classes of electron transporting polymers with the above characteristics need to be synthesized. I have developed a new straightforward synthetic strategy to rapidly generate new classes of electron transporting polymers with different chemical and electronic structure, broad absorption range, and high electron mobility from readily available electron deficient monomers. In APSCs due to low entropy of mixing, polymers tend to micro-phase segregate rather than forming the more useful nano-phase segregation. Optimizing the polymer blend morphology to obtain nano-phase segregation is specific to the system under study, time consuming, and not trivial. Thus to avoid micro-phase segregation, nanoparticles of hole and electron transporters are synthesized and blended. But the PCE of nanoparticle blends are far less than those of polymer blends. This is mainly due to the: i) lack of optimal assembly of nanoparticles to facilitate charge transfer and transport processes; and ii) lack of control over the exciton and charge transport properties within the nanoparticles. Polymer packing within the nanoparticle controls the optoelectronic and charge transport properties of the nanoparticle. In this work I have shown that the solvent used to synthesize nanoparticles plays a crucial role in determining the assembly of polymer chains inside the nanoparticle there by affecting its exciton and charge transport processes. To obtain the optimal morphology for better charge transfer and transport, we have also synthesized nanoparticles of different radius with surfactants of opposite charge. We propose that depending on the radius and/or Coulombic interactions these nanoparticles can be assembled into mineral structure-types that are useful for photovoltaic devices.

京都大学 / 新制・課程博士 / 博士(人間・環境学) / 甲第24149号 / 人博第1052号 / 新制||人||246(附属図書館) / 2022||人博||1052(吉田南総合図書館) / 京都大学大学院人間・環境学研究科相関環境学専攻 / (主査)教授 内本 喜晴, 教授 吉田 鉄平, 准教授 松井 敏明, 教授 林 晃敏 / 学位規則第4条第1項該当 / Doctor of Human and Environmental Studies / Kyoto University / DFAM

This study aims to explore strengths and challenges in an all-female executive team. The subject of female executive teams is relatively unexplored, why the study contributes with a new perspective on the dynamics and processes occurring in an all-female executive team. 45-minute interviews were conducted with each member of the group, which consists of five women between the age of 50 and 61. Together they constitute an executive team in a Swedish organization. A thematic analysis was conducted and four themes were identified, as follows: openness, respect, inefficient processes and homogeneity. In general, the group is perceived to be well-functioning, and they consistently emphasize positive qualities within their group. Among other things, they talk about an open climate, absence of prestige and express care for each other. To some extent, the group is homogeneous. This makes them at risk of groupthink and missing perspectives, although there is no indication in the interviews that this is occurring. They do however express a lack of time and strategic work, deficiencies in structure and to some extent a too large need for control. The study’s findings are discussed in relation to previous research on power structures, gender studies and group development. At last, suggestions are given for further studies to explore whether it is the gender composition or the women's personalities that make the group so well-functioning.

Creation of visually-pleasing images has always been one of the main goals of computer graphics. Two important components are necessary to achieve this goal --- artists who design visual aspects of an image (such as materials or lighting) and sophisticated algorithms that render the image. Traditionally, rendering has been of greater interest to researchers, while the design part has always been deemed as secondary. This has led to many inefficiencies, as artists, in order to create a stunning image, are often forced to resort to the traditional, creativity-baring, pipelines consisting of repeated rendering and parameter tweaking. Our work shifts the attention away from the rendering problem and focuses on the design. We propose to combine non-physical editing with real-time feedback and provide artists with efficient ways of designing complex visual aspects such as global illumination or all-frequency shadows. We conform to existing pipelines by inserting our editing components into existing stages, hereby making editing of visual aspects an inherent part of the design process. Many of the examples showed in this work have been, until now, extremely hard to achieve. The non-physical aspect of our work enables artists to express themselves in more creative ways, not limited by the physical parameters of current renderers. Real-time feedback allows artists to immediately see the effects of applied modifications and compatibility with existing workflows enables easy integration of our algorithms into production pipelines.

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