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Identifica??o e quantifica??o de hem?citos de f?meas ingurgitadas de Boophilus microplus inoculados com fungos Metarhizium anisopliae, Beauveria bassiana, Penicillium corylophilum e Fusarium oxysporum. / Identification and quantification of hemocytes obtained from engorged females of Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae) inoculated with Metarhizium anisopliae, Beauveria bassiana, Penicillium corylophilum and Fusarium oxysporumSilva, Sandra Borges da 24 February 2006 (has links)
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Previous issue date: 2006-02-24 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Biological control of the tick Boophilus microplus with Metarhizium anisopliae and
Beauveria bassiana has been evaluated by several researchers. The selection of
specimens resistant to chemical products is a mechanism used by arthropods for
survival and maintenance in the environment. It is already known how fungi penetrate
the host and their pathogenic potential at different phases of their biological cycle,
however, ticks immune response against these agents need more detailed studies. The
present work had as objectives: to identify and quantify the cellular types involved in
the cellular response of B. microplus inoculated with entomopathogenic (M. anisopliae
and B. bassiana) and non-entomopathogenic fungi (Penicillium corylophilum and
Fusarium oxysporum). In this study 60 engorged females of B. microplus were used,
representing six treatment groups with 10 specimens each. Ticks were inoculated with
aqueous suspension of conidia. There were two control groups: in the first one ticks
were inoculated with Tween 80 0,1% aqueous solution (negative control), in the second
one ticks were not inoculated (testimony group). Fungi suspension or Tween 80 solution
were applied in the back part of the idiosoma of the tick. The hemolinfa samples were
collected during the whole period of life of the specimens, beginning 24 hours after
inoculation. The haemolymph samples were fixed in methanol and stained with Giemsa.
In all studied periods, cells like pro-hemocytes, plasmatocytes, granulocytes,
spherulocytes and oenocytoids were observed in the specimens of all groups. Prohemocytes,
plasmatocytes and spherulocytes were the most numerous cells observed in
the hemolinfa. Hemocytes were absent in the group inoculated with B. bassiana 72
hours after inoculation. After this period, specimens inoculated with entomopathogenic
fungi were dead. The absence of cells suggests the immune-suppressor effect of the
fungi on this tick species. It was not observed when ticks were treated with M.
anisopliae due to accentuated mortality caused by this fungus species. The nonentomopathogenic
fungi did not affect the life cycle of this tick, being quickly
eliminated of the organism. It was suggested based on the absence of conidia during
sampling. / O controle biol?gico do carrapato Boophilus microplus com a utiliza??o de Metarhizium
anisopliae e Beauveria bassiana tem sido avaliado por diversos pesquisadores. A
sele??o de cepas resistentes ? um mecanismo adotado pelos artr?podes para
sobreviv?ncia da esp?cie e sua manuten??o no ambiente. Apesar de estar esclarecida a
forma como o fungo penetra no hospedeiro e comprovada sua patogenicidade sobre
diferentes fases de seu ciclo biol?gico, a sua resposta imune frente a estes agentes
agressores necessita de maiores estudos. O presente trabalho teve como objetivo:
identificar e quantificar os tipos celulares envolvidos na resposta imune celular de B.
microplus inoculados com fungos entomopatog?nicos n?o entomopatog?nicos. Na
experimenta??o foram utilizados 60 f?meas ingurgitadas de Boophilus microplus,
representando seis tratamentos cada um contendo 10 esp?cimes. Para os grupos
inoculados com fungos entomopatog?nicos foi utilizada a suspens?o aquosa dos fungos
Metarhizium anisopliae (isolado Ma 959) e Beauveria bassiana (isolado Bb 986). Nos
tratamentos com fungos n?o entomopatog?nicos, Penicillium corylophilum e Fusarium
oxysporum, foram formados ainda um grupo testemunha (n?o recebeu inocula??o) e um
grupo inoculado com solu??o de tween 80 a 0,1% em ?gua destilada est?ril, considerado
controle negativo. Nos grupos dos tratamentos com suspens?o f?ngica ou solu??o a
inocula??o foi feita na regi?o posterior do idiossoma do carrapato. As coletas de
hemolinfa foram realizadas durante todo o per?odo de vida dos esp?cimes, tendo inicio
24 horas ap?s inocula??o. As amostras de hemolinfa foram fixadas com metanol e
coradas com Giemsa. Em todos os per?odos estudados, tanto nos esp?cimes inoculados
com fungos como nos controles, foram observados pr?-hem?citos, plasmat?citos,
granul?citos, esferul?citos e oenocit?ides. Pr?-hem?citos, plasmat?citos e esferul?citos
foram ?s c?lulas mais numerosas na hemolinfa. Foi observada a aus?ncia de hem?citos
no grupo inoculado com B. bassiana 72 horas ap?s a inocula??o, como tamb?m a morte
dos esp?cimes inoculados com fungos entomopatog?nicos a partir deste per?odo. A
aus?ncia de c?lulas evid?ncia o efeito imunossupressor do fungo sobre os carrapatos
estudados. Caracter?stica n?o observada para Metarhizium anisopliae, mesmo tendo
provocado acentuada mortalidade dos carrapatos. Os fungos n?o entomopatog?nicos
n?o afetaram de forma significativa o ciclo de vida destes carrapatos, sendo rapidamente
eliminados do organismo.
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Avalia??o dos Perfis Prot?ico e Lip?dico na Resposta de Rhipicephalus Microplus ? Infec??o com Fungos. / Evaluation of protein and lipid profile in response of Rhipicephalus microplus to infection by fungi.Angelo, Isabele da Costa 03 March 2011 (has links)
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Previous issue date: 2011-03-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The study evaluated the protein and lipid profiles of Rhipicephalus microplus engorged
females after infection by Metarhizium anisopliae, Beauveria bassiana and Fusarium
oxysporum. The treatments were immersion or inoculation of conidial suspension in R.
microplus. The hemolymph was collected 24 and 48 hours after treatment. The cell-free
hemolymph was separated of hemocytes by centrifugation and hemocytes resuspended in
phosphate buffer pH 7.2. The amount of total protein was determined in both fractions of
hemolymph and hemocytes were quantified. The cell-free hemolymph was filtered through a
100 kDa and 10 kDa membranes, and analyzed by electrophoresis and liquid chromatography
(HPLC). The proteome of cell-free hemolymph (treatment by injection) was evaluated by 2DPAGE.
Changes were observed in amount total protein and the amount of hemocytes, but no
difference was observed in the electrophoretic profile (1D-PAGE) of the cell-free
hemolymph. In haemocytes, the entomopathogens reduced the amount of serpins, while F.
oxysporum caused increased. In 2D-PAGE variations were observed in both expression and
presence/absence of protein between the groups. The cell-free hemolymph antimicrobial
activity was tested against Escherichia coli and Staphylococcus aureus and against the fungus
used in the treatment of engorged ticks. The hemolymph collected 48 hours after the
immersion treatment with B. bassiana apresented activity anti-B. bassiana with 48 hours of
evaluation. This hemolymph was subjected to Superose column to HPLC and peak was
collected and analyzed on the analytical column C18. The fractions were collected from the
C18 and its apresented activity anti-B. bassiana, but showed no activity against Candida
albicans. These fractions were analyzed by Maldi-Tof and most of them had in common an
ion with m/z 1,119.5; however, other ions may be involved with this activity antimicrobial.
The lipids present in cell-free hemolymph, in the hemocyte and fat body were extracted and
analyzed by thin layer chromatography (TLC) or HPTLC for neutral lipids and phospholipids.
The classes of neutral lipids in the cell-free hemolymph were cholesterol ester, cholesterol
(CHO) and fatty acids (FA), which have varied depending on the fungus used, type of
treatment and observation time. Phospholipids found were phosphatidylcholine and
phosphatidylethanolamine, its were not significantly altered after the fungal infection. In
hemocytes, the same classes of lipids were found and B. bassiana modified phospholipids,
while M. anisopliae s.l. altered FA and CHO. The fat body showed, in addition to these
classes of neutral lipids, the triglycerides, which increased significantly 48 hours after
inoculation with M. anisopliae s.l. The lipase activity in fat body was measured and it was
demonstrated that increased activity 48 hours after inoculation, mainly in the group inoculated
with Metarhizium. Therefore, the results showed alterations related to the proteins expression
in the hemocytes and the cell-free hemolymph after inoculation with fungi,
immunosuppression of hemocytes and antimicrobial peptides induction after infection with B.
bassiana, besides changes in the lipid profile of R. microplus after infection. However, further
studies are necessary to understand these changes. / O trabalho avaliou os perfis prot?ico e lip?dico de f?meas ingurgitadas de Rhipicephalus
microplus ap?s infec??o com Metarhizium anisopliae s.l., Beauveria bassiana ou Fusarium
oxysporum. Os tratamentos foram imers?o ou inocula??o da suspens?o conidial em R.
microplus. A hemolinfa foi coletada 24 e 48 horas ap?s os tratamentos. O plasma foi separado
dos hem?citos por centrifuga??o e os hem?citos resuspensos em tamp?o fosfato pH 7,2. A
concentra??o de prote?na total foi determinada em ambas as fra??es da hemolinfa e os
hem?citos quantificados. O plasma da hemolinfa foi filtrado em membrana de 100 kDa e 10
kDa, sendo analisados por eletroforese e cromatografia l?quida de alta efici?ncia (CLAE). O
proteoma do plasma da hemolinfa (tratamento por inocula??o) foi avaliado por gel 2D. Foram
observadas varia??es na quantidade de prote?na total de ambas as fra??es da hemolinfa, na
quantidade de hem?citos bem como na intensidade de prote?nas/pept?deos expressos no
plasma da hemolinfa. Nos hem?citos, os entomopat?genos reduziram a quantidade de
serpinas, enquanto F. oxysporum causou aumento. No gel 2D foram observadas varia??es na
express?o bem como na aus?ncia/presen?a de prote?nas entre os grupos. O plasma da
hemolinfa teve sua atividade antimicrobiana testada contra Escherichia coli e Staphylococcus
aureus e contra o fungo utilizado no tratamento das f?meas ingurgitadas. A hemolinfa
coletada 48 horas ap?s o tratamento por imers?o com B. bassiana apresentou atividade anti-B.
bassiana com 48 horas de avalia??o. Esta hemolinfa foi submetida ? coluna Superose de
CLAE e o pico coletado analisado na coluna anal?tica C18. As fra??es coletadas da C18
apresentaram atividade anti-B. bassiana, por?m n?o apresentaram atividade contra Candida
albicans. Essas fra??es foram analisadas por Maldi-Tof e a maioria delas apresentou um ?on
com raz?o m/z 1.119,5; no entanto, outros ?ons podem estar envolvidos com essa atividade
antimicrobiana. Os lip?deos presentes no plasma da hemolinfa, nos hem?citos e no corpo
gorduroso foram extra?dos e analisados por cromatografia em camada delgada (CCD) ou
CCD de alta performance para lip?deos neutros e fosfolip?deos. As classes de lip?deos neutros
encontradas no plasma da hemolinfa foram colesterol-?ster, colesterol (CHO) e ?cidos graxos
(AG), que sofreram altera??es em fun??o do fungo utilizado, tipo de tratamento e tempo de
observa??o. Os fosfolip?deos encontrados foram fosfatidilcolina e fosfatidiletanolamina, que
n?o foram significativamente alterados ap?s a infec??o f?ngica. Nos hem?citos, as mesmas
classes de lip?deos foram encontradas e B. bassiana alterou os fosfolip?deos, enquanto M.
anisopliae s.l. alterou os AG e CHO. O corpo gorduroso apresentou, al?m destas classes de
lip?deos neutros, o triacilglicerol, que aumentou significativamente 48 horas ap?s a inocula??o
com M. anisopliae s.l. A atividade lipase no corpo gorduroso foi mensurada, sendo
evidenciado um aumento 48 horas ap?s a inocula??o, principalmente no grupo inoculado com
Metarhizium. Portanto, os resultados demonstraram altera??es na express?o de prote?nas no
plasma da hemolinfa e nos hem?citos ap?s inocula??o com os fungos, imunossupress?o dos
hem?citos, indu??o de pept?deos com atividade antimicrobiana ap?s infec??o com B.
bassiana, al?m de altera??es no perfil lip?dico de R. microplus ap?s infec??o. No entanto,
maiores estudos s?o necess?rios para o entendimento dessas altera??es.
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Avalia??o do modelo de gest?o e teorias de estrat?gia empresarial nas institui??es de ensino em Volta Redonda . / Grade of the model management and theories business strategy in the educational institutions in Volta Redonda.SILVA, Maricin?ia Pereira Meireles da 29 August 2008 (has links)
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Previous issue date: 2008-08-29 / The main aim of this dissertation consisted in examining the management model used in educational institutions. The pecific aims were: defining management in the educational process; comparing the strategic actions taken in schools surveyed; describig some models of business strategy and examining the possibilities of the application of some business strategies in schools; As research types the following models were combined: scientific, theoretical, descriptive and field search. As methods were used following: scientific, observational, deductive and comparive. The techniques articulated in the field search were the following: interviews, questionnaires and systematization of contents. From the theoretical practical analises of this research a particular vision of business strategies in the field of education in private company of basic education was taken. Issues as communication, interpersonal relations and management of people are points with different approaches in the educational area, since the school administration is a field that deserves attention and studies regarding the systematization in order to understand it better and, then locate concepts of business strategy a more conscious and scientific way.Finally, the certainty that the school learning is something of first need sends who is dealing with this branch of business to the responsibility of an effective operation, which becomes possible through understanding and comprehension of what is the most modern in administration. The educational institutions which were under study are located in Volta Redonda, a city that grew around Companhia Siderurgica Nacional (CSN). So, with large numbers of people who came, and still comes, in search of employment and chose and choose Volta Redonda to raise their families and build their stories. But with a crisis surrounding the industry, which was state-owned and was privatized in 1993, the place which provided to a some businessmen in the educational sector a fertile field has become a site of great competition in this school area, and now this is the time that only the best will be settled, which can be obtained from the established strategies. / O objetivo geral nesta disserta??o consistiu em analisar o modelo de gest?o adotado nas institui??es de ensino. Os objetivos espec?ficos foram os seguintes: definir gest?o no processo educacional; comparar a??es estrat?gicas adotadas nas escolas pesquisadas; descrever alguns modelos de estrat?gia empresarial e examinar as possibilidades da aplica??o de algumas estrat?gias empresariais em escolas. Quanto aos tipos de pesquisa, foram combinadas as seguintes modalidades: cient?fica, te?rica, descritiva e pesquisa de campo. Quanto aos m?todos, foram utilizados os seguintes: cient?fico, observacional, dedutivo e comparativo. As t?cnicas articuladas na pesquisa de campo foram as seguintes: entrevistas, question?rios e sistematiza??o de conte?dos. A partir da an?lise te?rica pr?tica desta pesquisa, obteve-se uma vis?o particularizada das estrat?gias empresariais no campo da educa??o, de empresas privadas de educa??o b?sica. .Fatores como a comunica??o, as rela??es interpessoais e a gest?o de pessoas s?o pontos com abordagens diferentes na ?rea educacional, visto que a administra??o escolar ? um ramo que merece aten??o e estudos no que diz respeito ? sistematiza??o para que seja poss?vel compreend?-la melhor e, ent?o, situar conceitos de estrat?gia empresarial, de uma forma mais consciente e cient?fica. Por fim, a certeza de que o ensino escolar ? um g?nero de primeira necessidade remete os que lidam com esse ramo empresarial ? responsabilidade de um funcionamento eficaz, o que se torna vi?vel por meio da compreens?o e aplica??o do que h? de mais moderno em administra??o. As institui??es educacionais que foram objeto de estudo situam-se em Volta Redonda, cidade que e cresceu em torno da Companhia Sider?rgica Nacional (CSN). Portanto com grande n?mero de pessoas que vieram, e ainda vem, em busca de emprego e a escolheram e escolhem para criar suas fam?lias e construir suas hist?rias. Por?m, com uma crise em torno da ind?stria, que era estatal e se privatizou em 1993, o lugar, o qual proporcionou a alguns empres?rios no ramo educacional um campo f?rtil, tem se tornado um espa?o de grande competi??o nessa ?rea escolar, chegando a hora de se firmar apenas as melhores, o que poder? ser obtido em fun??o das estrat?gias estabelecidas.
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Avalia??o experimental de Lecanicillium lecanii no controle biol?gico de Stomoxys calcitrans / Experimental evaluation of Lecanicillium lecanii in biological control of Stomoxys calcitransALVES, Paula Sant?ana 26 February 2010 (has links)
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Previous issue date: 2016-02-26 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPQ / Stomoxys calcitrans is a hematophagous fly of which parasitism causes economic
losses related to the reduction of milk production and weight gain. Due to the high
level of resistance of the stable fly to most of chemical insecticides, it is necessary to
search for new alternatives for their control, and one of them would be the use of
entomopathogenic fungi. However, few researches related to microbial control of this
fly were carried out. The objective of this study was to assess the ability of the
Lecanicillium lecanii fungus in to make unfeasible eggs, larvae and pupae of the stable
fly under laboratory conditions and verify the formation of inhibition zone in agar
solid due to the presence of substances in the mucus and total homogenized larvae not
previously exposed and sensitized to the fungus. The S. calcitrans colony was
maintained in the Laborat?rio de D?pteros Hemat?fagos, UFRRJ. The tests were
performed in the Laborat?rio de Controle Microbiano de Artr?podes, UFRRJ, where
the strain CG 420 of L. lecanii was used to prepare the water suspensions. In assays
with eggs, larvae and pupae of S. calcitrans, fungal suspensions were used at 2x108,
2x107, 2x106, 2x105 con.mL-1 concentrations, besides the positive control. In assays
with eggs, they were immersed in different fungal suspension and transferred to Petri
dishes with paper filter, which were added larval rearding medium and its respective
suspension. Mortality was assessed five days after exposure, where it was observed
whether stage change occurred. There was no significant difference in the hatching of
eggs exposed to different fungal concentrations against the control. Larvae were
subjected to the same treatment of eggs, and mortality was assessed ten to fifteen days
after exposure. There was no significant difference in mortality of larvae exposed to
the fungus when compared with the control group, however, the fungus L. lecanii was
significantly able to reduce adult emergence from larvae exposed to the highest tested
concentration and there was a percentage of emergence of 25,45% and 43,64%
compared to 63,64% and 90,91% of the control group. The pupae were also immersed
in different fungal suspensions, and transferred to Petri dishes with paper filter, added
to its respective suspension. Mortality was assessed ten days after exposure. There was
no significant difference in mortality of pupae exposed to different fungi
concentrations. Filter paper discs were soaked in mucus samples and total
homogenized from larvae of S. calcitrans not exposed and previously sensitized to the
fungus L. lecanii and placed in petri dishes sown with the same fungus. It was not
observed the formation of inhibition zone around the discs. The L. lecanii fungus was
unable to make unfeasible eggs, larvae or pupae of S. calcitrans in tested fungal
concentrations, however, was significantly able to reduce the emergence of adults
when their third larvae stage were exposed to 2x108 con.mL-1 concentration. / Stomoxys calcitrans ? um d?ptero hemat?fago cujo parasitismo ocasiona perdas
econ?micas relacionadas ? redu??o da produ??o leiteira e ganho de peso, al?m de estar
associado ? dissemina??o e transmiss?o de agentes patog?nicos. Devido ao elevado
grau de resist?ncia da mosca dos est?bulos a uma diversidade de inseticidas qu?micos,
torna-se necess?rio a busca por alternativas para o seu controle, e uma delas seria a
utiliza??o de fungos entomopatog?nicos. Todavia, poucas pesquisas relacionadas ao
controle microbiano desta mosca foram realizadas. O objetivo deste estudo foi
verificar a capacidade do fungo Lecanicillium lecanii em inviabilizar ovos, larvas e
pupas de S. calcitrans em condi??es laboratoriais e verificar a forma??o de halo de
inibi??o em meio de cultivo s?lido, em decorr?ncia de subst?ncias presentes no muco
e no macerado total de larvas n?o expostas e previamente sensibilizadas ao fungo. A
col?nia de S. calcitrans foi mantida no Laborat?rio de D?pteros Hemat?fagos, UFRRJ.
Os ensaios foram realizados no Laborat?rio de Controle Microbiano de Artr?podes,
UFRRJ. Nos ensaios foram utilizadas suspens?es aquosas do isolado CG 420 de L.
lecanii nas concentra??es de 2x108, 2x107, 2x106, 2x105 con.mL-1, al?m do controle
positivo. Nos ensaios com ovos, estes foram imersos nas suspens?es f?ngicas, e
transferidos para placas de Petri com papel filtro, meio de desenvolvimento larval e 3
mL de sua respectiva suspens?o. A mortalidade foi avaliada cinco dias ap?s a
exposi??o. N?o foi observada diferen?a significativa na eclos?o dos ovos tratados
frente ao controle. As larvas foram submetidas ao mesmo tratamento dos ovos, e a
mortalidade foi avaliada dez e quinze dias ap?s a exposi??o. N?o foi observada
diferen?a significativa na mortalidade de larvas expostas ao fungo quando comparados
com o grupo controle, entretanto, o fungo L. lecanii reduziu significativamente a
emerg?ncia de adultos provenientes das larvas expostas ? concentra??o m?xima
testada (2x108 con.mL-1) sendo observado um percentual de emerg?ncia de 25,45% e
41,82 %, em compara??o com 63,64% e 90,91% do grupo controle. As pupas tamb?m
foram imersas nas suspens?es f?ngicas, e transferidas para placas de Petri com papel
filtro e sua respectiva suspens?o. A mortalidade foi avaliada dez dias ap?s a
exposi??o. N?o foi observada diferen?a significativa na mortalidade das pupas
expostas ?s diferentes concentra??es f?ngicas. Discos de papel filtro foram embebidos
em amostras de muco e macerado total proveniente de larvas de S. calcitrans n?o
expostas e previamente sensibilizadas ao fungo L. lecanii e colocados em placas de
Petri semeadas com o mesmo fungo (1x108 con.mL-1). N?o foi verificada a forma??o
de halo de inibi??o ao redor dos discos. O fungo L. lecanii foi incapaz de inviabilizar
ovos, larvas ou pupas de S. calcitrans nas concentra??es f?ngicas testadas, no entanto,
reduziu significativamente a emerg?ncia de adultos quando suas larvas de terceiro
est?dio foram expostas a concentra??o 2x108 con.mL-1.
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Organiza??o do trabalho pedag?gico dos professores do IFAM - Campus s?o Gabriel da cachoeira: um estudo das concep??es pedag?gicas que fundamentam sua pr?tica docente. / Organization of work of teachers teaching the IFAM - campus s?o gabriel da cachoeira: a study of the pedagogical assumptions that underlie their teaching practiceSilva, Josiani Mendes 04 February 2011 (has links)
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Previous issue date: 2011-02-04 / The Federal Office for Education, Science and Technology of the Amazon - Campus S?o Gabriel da Cachoeira is located in the upper Rio Negro, Amazon, where indigenous people live in various ethnic groups with different languages, traditions, customs and traditions. Among the many challenges that are brought to this campus for the construction of an education for all, enabling universal access to knowledge and value, while the practices and traditional knowledge of indigenous peoples, is the qualification of teachers in planning mediation of construction of the teaching-learning of indigenous students at the school even other non-indigenous students. The study presented here aimed to investigate which pedagogical concepts teachers use as a basis for organizing their pedagogical work. This is a descriptive characterized as a case study, conducted at the Federal Institute of Education, Science and Technology of the Amazon - Campus S?o Gabriel da Cachoeira, with eleven professors from the Technical Course in Agriculture, which administer the technical disciplines in-vocational education in indigenous communities, as well as on campus. We collected data from four phases of work: Step 1: We did a literature search, which was backed by the theoretical background on the authors to address issues relevant to this study; Step 2: We performed the fieldwork with the course faculty Farming Technician, through questionnaires on educational concepts in school practice as well as on their professional profile; Step 3: analyze their teaching plans, which allowed us to observe how they planned their teaching practice through methodological procedures, the resources that would be used for lessons and evaluation tools; Step 4: We used participant observation to describe the involvement of teachers in the construction of Project Political campus. The material was interpreted from the theoretical framework adopted in which content analysis was used for this purpose. In seeking to understand how teachers organized both his pedagogical work, such as the Educational Policy Project of the campus, we realize that they do not understand that there is a relationship between education and society, therefore, have no understanding of the meaning of education to society where the campus is inserted and therefore there is a pedagogical concept defined the reasons for organizing their pedagogical work. To meet the multi-ethno-cultural in that region, allowing a differentiated model of education, constant updating is a fundamental need and unambiguous, and therefore stress the importance of such training to promote the continuous development of teachers, with actions that lead to investigate, search and interact, enabling the expansion of their knowledge and resilience, both in its design in relation to education, the challenges and problems of their everyday school life. / O Instituto Federal de Educa??o, Ci?ncia e Tecnologia do Amazonas ? Campus S?o Gabriel da Cachoeira situa-se no alto Rio Negro, Amazonas, onde convivem povos ind?genas de v?rias etnias com diferentes l?nguas, usos, costumes e tradi??es. Entre os muitos desafios que est?o postos a este campus para a constru??o de uma educa??o para todos, que possibilite acesso aos conhecimentos universais e valorize, ao mesmo tempo, as pr?ticas e saberes tradicionais das popula??es ind?genas, est? o da qualifica??o dos professores em planejar a media??o da constru??o do processo ensino-aprendizagem de alunos ind?genas, no mesmo espa?o escolar de outros alunos n?o ind?genas. O estudo aqui proposto teve por objetivo investigar quais concep??es pedag?gicas os professores utilizam como base para a organiza??o do seu trabalho pedag?gico. Trata-se de uma pesquisa descritiva caracterizada como um estudo de caso, realizada no Instituto Federal de Educa??o, Ci?ncia e Tecnologia do Amazonas ? Campus S?o Gabriel da Cachoeira, com onze professores do Curso T?cnico em Agropecu?ria, os quais ministram disciplinas da ?rea t?cnico-profissionalizante em comunidades ind?genas, assim como no campus. Realizamos a coleta de dados a partir de quatro etapas de trabalho: Etapa 1: fizemos uma pesquisa bibliogr?fica, onde houve o respaldo do embasamento te?rico nos autores que tratam das quest?es pertinentes ao presente estudo; Etapa 2: realizamos a pesquisa de campo com os professores do curso T?cnico em Agropecu?ria, por meio de question?rios sobre as concep??es pedag?gicas em sua pr?tica escolar, bem como sobre o seu perfil profissional; Etapa 3: analisamos seus planos de ensino, os quais nos permitiram observar como planejavam sua pr?tica pedag?gica, atrav?s dos procedimentos metodol?gicos, os recursos que seriam utilizados para as aulas e os instrumentos de avalia??o; Etapa 4: utilizamos a observa??o participante para descrever a participa??o desses professores na constru??o do Projeto Pol?tico Pedag?gico do campus. O material coletado foi interpretado a partir do marco te?rico adotado onde utilizamos a an?lise de conte?do para esse fim. Ao procurarmos conhecer como os professores organizaram, tanto seu trabalho pedag?gico, como o Projeto Pol?tico Pedag?gico do campus, percebemos que os mesmos n?o entendem que h? uma rela??o entre educa??o e sociedade, em consequ?ncia, n?o possuem um entendimento do sentido da educa??o para a sociedade em que o campus est? inserido e, portanto, n?o existe uma concep??o pedag?gica definida que fundamente a organiza??o de seu trabalho pedag?gico. Para atender a diversidade etno-pluri-cultural dessa regi?o, viabilizando um modelo de educa??o diferenciada, a atualiza??o permanente ? uma necessidade fundamental e inequ?voca, portanto, enfatizamos a import?ncia dessa forma??o para promover o desenvolvimento cont?nuo dos professores, com a??es que o levem a investigar, pesquisar e interagir, possibilitando a amplia??o de seus conhecimentos e a supera??o, tanto de sua concep??o em rela??o ? educa??o, quanto dos desafios e problemas do seu cotidiano escolar.
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Curso de tecnologia em produ??o de cacha?a: representa??o social dos estudantes sobre a cacha?a e o consumo de ?lcool / Course of technology in the production of cacha?a: social representation of students on the cacha?a and alcohol consumptionOliveira, Pedro Paulo 30 June 2011 (has links)
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Previous issue date: 2011-06-30 / Secretaria de Educa??o Profissional e Tecnologica - Setec/MEC. / Social Representations are structures of individual knowledge that are shared and that enable
a group understand the reality, to maintain their integrity and support their practices. This
work had the purpose to know the Social Representations of Cacha?a and its relationship with
the consumption of alcohol for students in the course of Technology in the Production of
Cacha?a of Federal Institute of the North of Minas Gerais (IFNM) -Campus Salinas. Using
the diagnosis of factors that had motivated these young people for this college course, to
identifying if the consumption and the conceptions about cacha?a at these young people
were affected by this college course, as well as the factors that the students has related for to
drinking cacha?a as: occasions, friendship, the correlation with the family habits, the risks to
health and social problems. The research was carried out in IFNMG, in the year 2010, with
forty-eight students in the upper course of Technology in the Production of Cacha?a. It was
consisted of three stages: 1- socioeconomic characteristics of students, with structured
questions; 2- Evaluation of the knowledge of the students about cacha?a that understood the
use of a Likert scale of five points; 3- Social representation of the cacha?a with roadmap built
with open-ended questions, in order to encourage the discourse, and the interviews were
recorded, transcribed and analyzed by technique of the Collective Subject Discourse, on the
basis of the theory of Social Representations, using the software Qualiquantisoft. Thus, it was
found that the universe of the students presents a predominance of women (62.5 %) and the
age range of 18 to 21 years old (56.2 % ). The majority (87 % ), concluded the middle school
at a public school in regular course (90 %) and are living in an urban area (81 %) in the city of
Salinas (86 % ). On the social representations for the consumption of alcohol, the students
have attitudes predominantly unfavorable to the consumption of alcoholic beverages; they
hadn't believe that the young people has drunk alcohol for "to feel sad or annoying" or "feel
more integrated and identified with their group"; the motivation more favorable on the
consumption of alcoholic beverages were "to relax or calm down the nerves" and "to feel
more adults". In terms of Knowledge and Beliefs, the students believe that alcohol can cause
physical dependence" and the consumption of cacha?a "helps to unwind/ encourage". It was
verifie that the social representations of students about the properties and the consumption of
alcoholic beverage and cacha?a, presents a great level of information and they has attitudes
predominantly negative in relation to alcohol consumption. The representation to the
attraction of the cacha?a from Salinas, it was found that "The fame of the cacha?a and the
city" as the most prevalent among the answers. Therefore, for these students, the biggest
attraction of cacha?a of Salinas maintains a direct correlation with the marketing that has
been attributed to the cacha?a this region. However, they have believed on the factors of
quality of the cacha?a once they showed an expressive representation in the categories
involved in the production and quality as: "sensory attributes", "quality" and "manufacture
craft". / As Representa??es Sociais s?o estruturas de conhecimento individuais, compartilhadas, que
permitem a um grupo entender uma realidade, manter sua integridade, direcionar e
fundamentar suas pr?ticas. Esta pesquisa teve como prop?sito conhecer as Representa??es
Sociais da Cacha?a e a sua rela??o com o consumo de ?lcool para alunos do curso superior
Tecn?logo em Produ??o de Cacha?a do no Instituto Federal do Norte de Minas Gerais
(IFNM)-Campus Salinas, atrav?s do diagn?stico dos fatores que motivaram o ingresso desses
jovens neste curso; identificando se o consumo de e as concep??es sobre cacha?a pelos jovens
foram afetados pelo seu ingresso no curso, bem como os fatores que os alunos relacionam
com ato de beber cacha?a como: ocasi?es, companhias, a correla??o com os h?bitos
familiares, os riscos ? sa?de e problemas sociais. A pesquisa foi realizada no IFNMG, no ano
de 2010, com quarenta e oito alunos do curso superior de Tecnologia em Produ??o de
Cacha?a e consistiu de tr?s etapas: 1-a caracteriza??o socioecon?mica dos discentes com um
roteiro estruturado; 2-a avalia??o do conhecimento dos alunos sobre cacha?a que
compreendeu a utiliza??o de uma escala do tipo Likert de cinco pontos; 3- a pesquisa de
representa??o social da cacha?a com roteiro constru?do com quest?es abertas, a fim de
incentivar o discurso, sendo os discursos gravados, transcritos e analisados pela t?cnica do
Discurso do Sujeito Coletivo, com base na teoria das Representa??es Sociais, utilizando o
software Qualiquantisoft. Assim, verificou-se que o universo dos sujeitos do presente estudo
apresenta um predom?nio de mulheres (62,5%) e a faixa et?ria de 18 a 21 anos de idade
(56,2%). A sua maioria (87%), concluiu o ensino m?dio em escola p?blica, em curso regular
(90%) e s?o residentes em ?rea urbana (81%) no munic?pio de Salinas (86%). Nas
representa??es sociais sobre o consumo de ?lcool, os sujeitos desta pesquisa apresentam
atitudes predominantemente desfavor?veis ao consumo de bebidas alco?licas; n?o acreditam
que os jovens bebam por ?se sentirem tristes ou aborrecidos? ou para ?se sentirem mais
integrados e identificados com o seu grupo?; as motiva??es mais favor?veis sobre o consumo
de bebida alco?lica foram ?para relaxar ou acalmar os nervos? e ?para se sentirem mais
adultos?. Quanto ao Conhecimento e Cren?as eles acreditam que o ?lcool ?pode causar
depend?ncia f?sica? e o consumo de cacha?a ?ajuda a descontrair/ desinibir?.
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Planejamento Estrat?gico no Ambiente de Redes Interorganizacionais: um estudo comparativo. / Strategic Planning in Inter-Worknets Environment: a comparative study.Lima, Rafael de Paiva 14 August 2008 (has links)
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Previous issue date: 2008-08-14 / The present work analyses the traditional enterprise environment and the environment of
inter-worknets networks when elaborating the Strategic Planning (SP). For this, start with the
premise that the main topic of organizational change, at the last decades, includes the
structure of organizations. These changes are a reaction of the organizations to the
merchandizing intense pressures that amplified the level of competitivity. In such case, the
establishment of contributing relations appeared as a strong tool of organizational
development, however caused a change on the way of observing the enterprises. Under this
context, the traditional strategic planning becomes an analyzed target in virtue of this new
organizational environment that is formed when the conception of the structures at networks
of enterprises. The general objective in this dissertation consist to promote a comparative
analysis of the process of Strategic Planning (SP) between an enterprise of traditional pattern
and an inter-worknets networks, in function of the contrast among the organizational
environments. For this, will be analyzed the construction of the strategic planning at a
traditional enterprise environment (only one enterprise) establishing a comparison with the
same process at an inter-worknet network (enterprises in network). It is suppose that the
network organizational environment differs from the traditional organizational environment.
This way, the process of strategic planning is different by strength of structural divergence
among the organizational environments. The objective of this study is to explain possible
divergences at the elaboration of the SP between the environments in this study called:
traditional and inter-worknets networks. Supplementary the study presume that the process of
strategic planning permit the access of information and privileged recourses that processed
enables the development of the knowledge of organizations and becomes an important tool to
the organizational development. Such suppositions and objectives were reached based on a
structured questionary and personal interview with managers of Strategic Planning of the two
researched enterprises. Both of them are recognized on the national and international scenario
representing the relevance of the study. / O presente trabalho analisa o ambiente empresarial tradicional e o ambiente de redes
interorganizacionais quando da elabora??o do planejamento estrat?gico (PE). Para tal, partese
da premissa de que o principal t?pico de mudan?a organizacional, nas ?ltimas d?cadas,
envolve a estrutura das organiza??es. Tais mudan?as s?o rea??es das organiza??es ?s fortes
press?es mercadol?gicas que elevaram o n?vel de competitividade. Assim, o estabelecimento
de rela??es colaboradoras surgiu como forte ferramenta de desenvolvimento empresarial,
entretanto acarretou na mudan?a da forma com que se observam as empresas. Neste contexto,
o planejamento estrat?gico tradicional torna-se alvo de an?lise em virtude deste novo
ambiente organizacional que se forma quando da concep??o de estruturas em redes de
empresas. O objetivo geral nesta disserta??o consiste em promover uma an?lise comparativa
do processo de Planejamento Estrat?gico (PE) entre uma empresa de formato tradicional e
uma rede interorganizacional, em fun??o do contraste entre os ambientes organizacionais.
Para tanto, ser? analisada a constru??o do planejamento estrat?gico em um ambiente
empresarial tradicional (uma ?nica empresa) estabelecendo uma compara??o com o mesmo
processo em uma rede interorganizacional (empresas em rede). Sup?e-se que o ambiente
organizacional em rede difere-se do ambiente organizacional tradicional. Desta forma, o
processo de planejamento estrat?gico ? diferenciado por for?a da diverg?ncia estrutural entre
os ambientes organizacionais. O objetivo do presente estudo ? interpretar poss?veis
diverg?ncias na elabora??o do PE entre os ambientes neste estudo denominados: tradicional e
redes interorganizacionais. Complementarmente o estudo sup?e que este processo de
planejamento estrat?gico permite o acesso a informa??es e recursos privilegiados que
processados possibilitam o desenvolvimento do conhecimento das organiza??es e, portanto,
torna-se ferramenta importante para o desenvolvimento organizacional. Tais suposi??es e
objetivos foram atingidos com base em question?rio estruturado e entrevista pessoal com os
gestores de Planejamento Estrat?gico das duas empresas pesquisadas. Ambas s?o
reconhecidas no cen?rio nacional e internacional representando a relev?ncia do estudo.
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Forma??o t?cnica em agroind?stria: percep??es dos egressos que atuam na agroind?stria familiar de Venda Nova do Imigrante / Technical training in agroindustry: graduates' perceptions of the job in the family agroindustry of Venda Nova do ImigrantePALMEIRA, Jonadable Alves 21 March 2016 (has links)
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Previous issue date: 2016-03-21 / This survey was conducted in the city of Venda Nova do Imigrante with the graduates from the agroindustry technical course of the Instituto Federal do Esp?rito Santo (IFES). Aiming to analyze the pedagogic project of the course and the graduates' perception about their technical training. It was used a qualitative approach and turned to case study research, as well as basing on documentary analysis and observation. Started from literature review to support the analysis of educational legislation, IFES institutional documents and the pedagogical project of the course. With the theoretical basis consolidated, semi-structured interviews were done to nine participants, the interviews were subjected to content analysis, through technical analysis category. Understanding that the pedagogic project has two dimensions, the writing and the experienced, it started in a first moment with critical reflection of the written document and secondly to give contours to the pedagogic project experienced in the graduates' perception about their training. The results of this survey showed that on the way to a democratic school it is necessary to overcome the purely academic vision, the dialogue between the academic and the traditional knowledge may resignify the role of training in agroindustry, expanding the participation of the subjects and also enabling the construction of a effective collective project. / Esta pesquisa foi realizada no munic?pio de Venda Nova do Imigrante com os egressos do curso t?cnico em agroind?stria do Instituto Federal do Esp?rito Santo (IFES). Tendo como objetivo analisar o projeto pedag?gico do curso e a percep??o dos egressos quanto a sua forma??o t?cnica. Utilizou-se uma abordagem qualitativa e recorreu ao estudo de caso como tipo de pesquisa, al?m de se ancorar na an?lise documental e na observa??o. Partiu-se da revis?o de literatura para fundamentar a an?lise das legisla??es educacionais, dos documentos institucionais do IFES e do projeto pedag?gico do curso. Com a base te?rica consolidada, realizou-se entrevistas semi-estruturadas com nove participantes, as entrevistas foram submetidas a uma an?lise de conte?do, por meio da t?cnica de an?lise categoria. Compreendendo que o projeto pedag?gico tem duas dimens?es, o escrito e o vivenciado, partiu-se num primeiro momento da reflex?o critica do documento escrito e em um segundo momento buscou-se d? contornos ao projeto pedag?gico vivenciado a partir da percep??o dos egressos quanto a sua forma??o. Os resultados desta pesquisa apontaram que no caminho para uma escola democr?tica ? necess?rio superar a vis?o puramente acad?mica, o di?logo entre o conhecimento acad?mico e o saber tradicional poder? ressignificar o papel da forma??o em agroind?stria, ampliando a participa??o dos sujeitos e possibilitando assim a constru??o de um projeto efetivamente coletivo.
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Curr??culo da educa????o b??sica do Distrito Federal: um caminho para a express??o criativa do professor da escola p??blica?Teixeira, Cristiane Leal Alves 29 February 2016 (has links)
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Previous issue date: 2016-02-29 / This study investigated the ways and possibilities of development of the creative expression
of teachers of fourth and fifth grade from elementary school with the implementation of the
Basic Curriculum Moving the Distrito Federal Education. The research, with an emphasis on
quantitative and qualitative approaches, using the mixed method, being conducted from the
SEEDF documents, questionnaires, observation and semi-structured interviews. Twenty-nine
teachers attended six Regional Administrative schools from Plano Piloto/Cruzeiro. The
participants answered two questionnaires; three respondents were observed in teaching
activities with their classes and conducted the interview. The data were submitted to the
documentary analysis, descriptive statistics and content analysis. From the triangulation of
data held convergent analysis of the results. The data indicated that perceptions of teachers
about creativity construct refer to categories such as innovation element, the act of creating,
different to the producer and troubleshooting generator. Teachers (96%) reported not having
participated in training courses on creativity. On the other hand, 27,6% of respondents said
they had discussed this topic in pedagogical coordination. It is inferred from the evidence that
the perceptions presented by teachers on creativity come close to some descriptions reported
in the literature, but are linked to common sense expressions. No intentional pedagogical
actions that allow their own creative expression. Perceptions on Curriculum restricted to
guiding document, everything that goes on in the classroom, to the set of objectives and
contents. The results demonstrated the concern of the teacher to comply with the prescribed
content for their discipline. It is noteworthy, however, that less than 50% of respondents
teachers have participated in a training event on Curriculum Moving the Basic Education of
the Distrito Federal. Regarding the reading of books, 15 (51,5%) participants reported having
read the text on the theoretical assumptions and 22 (75,9%) Elementary School notebook
Early Years. The written document, the political-pedagogical projects of schools, according to
the results obtained, are not known to all teachers in schools investigated. In addition, the
flexibility of the proposed content, and the development of creativity in the classroom were
identified as factors dependent on the teacher's posture. The results also pointed to the need
for qualitative investment in the continuing education of teachers, both in relation to the
curriculum, and in relation to the creative process to be developed in the school context.
Flexibility was the most highlighted feature in the analysis of the documents by setting up as
an important element in building more creative teaching methods, flexible and humanized that
promote development and learning effectively student. / Este estudo investigou os caminhos e possibilidades de desenvolvimento da express??o criativa
do professor dos quartos e quintos anos do Ensino Fundamental, com a implementa????o do
Curr??culo em Movimento da Educa????o B??sica do Distrito Federal. A pesquisa, com ??nfase nas
abordagens quantitativa e qualitativa, utilizando o m??todo misto, foi conduzida a partir da
an??lise de documentos da SEEDF, aplica????o de question??rios, observa????o e entrevistas
semiestruturadas. Participaram 29 professores de seis escolas da Regional Administrativa do
Plano Piloto/Cruzeiro. Os participantes responderam a dois question??rios e destes, tr??s
respondentes foram observados em atividades docentes com suas turmas e realizaram a
entrevista. Os dados foram submetidos ?? an??lise documental, estat??stica descritiva e an??lise de
conte??do. A partir da triangula????o dos dados realizou-se a an??lise convergente dos resultados
obtidos, que indicaram que as percep????es dos professores acerca do construto criatividade
referem-se a categorias como: elemento de inova????o, ato de criar, produtora do fazer diferente
e geradora de solu????o de problemas. Os professores (96%) relataram n??o ter participado de
cursos de forma????o sobre criatividade. Por outro lado, 27,6% dos respondentes afirmaram ter
discutido sobre essa tem??tica nas coordena????es pedag??gicas. Infere-se a partir dessa
constata????o que as percep????es apresentadas pelos professores sobre criatividade aproximamse
de algumas descri????es relatadas na literatura, mas est??o vinculadas a express??es de senso
comum. N??o foram observadas a????es pedag??gicas intencionais que viabilizassem sua pr??pria
express??o criativa. As percep????es sobre curr??culo restringem-se a ideia de documento
norteador, tudo o que se passa em sala de aula, ao conjunto de objetivos e conte??dos. Os
resultados demonstraram a preocupa????o do professor em cumprir com o conte??do prescrito
para sua disciplina. Ressalta-se, no entanto, que menos de 50% dos professores respondentes
j?? participaram de algum evento de forma????o sobre o Curr??culo em Movimento da Educa????o
B??sica do Distrito Federal. Com rela????o ?? leitura dos cadernos, 15 (51,5%) participantes
afirmaram ter lido o texto sobre os Pressupostos Te??ricos e 22 (75,9%) o caderno de Ensino
Fundamental Anos Iniciais. O documento escrito dos Projetos Pol??ticos-Pedag??gicos das
escolas, de acordo com os resultados obtidos, n??o s??o conhecidos por todos os professores nas
institui????es escolares investigadas. Al??m disso, a flexibilidade dos conte??dos propostos, bem
como o desenvolvimento da criatividade em sala de aula foram apontados como fatores
dependentes da postura do professor. Os resultados apontaram ainda para a necessidade de
investimentos qualitativos na forma????o continuada dos professores, tanto em rela????o ao
curr??culo, quanto em rela????o ao processo criativo a ser desenvolvido no contexto escolar. A
flexibilidade foi a caracter??stica mais ressaltada na an??lise dos documentos, configurando-se
como um importante elemento na constru????o de pr??ticas pedag??gicas mais criativas, flex??veis
e humanizadas que promovam o desenvolvimento e aprendizagem do aluno de forma efetiva.
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Hiperatividade e d?ficit de aten??o : um olhar psicanal?ticoTuchtenhagen, Maria Beatriz Peixoto 22 March 2007 (has links)
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Previous issue date: 2007-03-22 / Este estudo busca analisar as manifesta??es de hiperatividade e de d?ficit de aten??o t?o popularizadas em nossos dias, sob a luz da psican?lise. Para tal foram elaborados dois artigos um te?rico e um emp?rico. O primeiro, Hiperatividade e D?ficit de Aten??o: um olhar psicanal?tico, ? uma revis?o da literatura sobre o assunto e visa situar esta tem?tica a partir do paradigma neurobiol?gico e do paradigma psicanal?tico. A revis?o te?rica sobre o tema aborda diferentes autores que trabalham esta problem?tica a partir de uma perspectiva psicanal?tica baseada nos aportes te?ricos de Freud a autores mais contempor?neos, que fazem uma releitura dos conceitos fundamentais da psican?lise como Jean Laplanche e S?lvia Bleichmar. Neste artigo ressalta-se a contribui??o que um olhar psicanal?tico pode proporcionar na compreens?o destas manifesta??es. Com o segundo artigo, Desaten??o e Hiperatividade: Destinos do sujeito?, busca-se destacar, atrav?s da ilustra??o de duas situa??es cl?nicas, a import?ncia que um diagn?stico metapsicol?gico tem para o entendimento destas manifesta??es comportamentais e sua compreens?o dos poss?veis sentidos que elas podem ter em cada situa??o. Este estudo demonstrou a import?ncia que uma abordagem que considera o sujeito em sua subjetividade e em sua singularidade pode oferecer para a compreens?o dos comportamentos de desaten??o e hiperatividade, e abrir as possibilidades de interven??o nestas situa??es.
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