Orientador: Célia Maria Guimarães / Resumo: A presente dissertação é resultado de uma pesquisa-ação sobre as contribuições que a formação contínua em serviço podem oferecer na construção da identidade do profissional de educação infantil. O local em que se deu a pesquisa foi o Centro de Convivência Infantil (CCI) Chalezinho da Alegria da Faculdade de Ciências e Tecnologia da UNESP de Presidente Prudente. O grupo de sujeitos enfocado é composto por 10 Recreacionistas e Auxiliares de Recreacionistas nomeadas "educadoras" no presente trabalho. O objetivo principal foi identificar e analisar os elementos do Programa de Formação Contínua em Serviço (PFCS) que ofereceram maior contribuição ao processo de identificação profissional das educadoras. Outro objetivo foi contribuir com a elaboração e redefinição do PFCS do CCI e de outras instituições de educação infantil. A partir dos dados obtidos através de entrevistas, observação participante natural, relato escrito e pesquisa documental foi observado um processo de modificação na identidade profissional das educadoras. Inicialmente identificaram-se com a figura da pajem ou babá e movimentaram-se constituindo a figura do profissional de educação infantil que desenvolve fazeres e saberes específicos. Os elementos do PFCS que mais contribuíram para essa mudança de identidade foram os espaços de formação, especialmente as orientações semanais, que permitiram tomar as situações do cotidiano como objeto de reflexão sobre a ação. / Abstract: This study is the result of a research-action on the contributions that the in-service continued formation can offer in terms of identity developing on infantile education professionals. This study was carried out at the Centro de Convivência Infantil (CCI) (Infantile Recreational Activity Center) Chalezinho da Alegria of the Science and Technology School (UNESP) in the city of Presidente Prudente. The emphasized professional teamwork is constituted of 10 recreational educators and recreational educator assistants entitled educationalists at the present study. The main purpose is to identify and analyze features of the in-Service Continued Formation Program (SCFP) that offers a major contribution for the professional identification process of educators. The contribution with the planing and redefining of the SCFP of CCI and others infantile educational institutions are also the purpose of this study. Based on data obtained from interviews, natural participant's observations, written report and documental research, it was noted a changing process on educationalists professional identity. At first, they are identified with baby sitter or nursemaid's image with movements that constitutes the infantile education professional image who develops specific skills and knowledge. The SCFP's features that had contributed most for this identity change are the formation's gaps, specially the weekly educational orientation which takes the daily situations as an reflective factor over the action. / Mestre

Orientador: João Luís Tápias Ceccantini / Banca: Edmir Perrotti / Banca: Juliana Silvia Loyola / Banca: Neuza Ceciliato / Banca: Odil José Oliveira Filho / Resumo: O presente estudo, organizado em dois volumes, analisa seis narrativas juvenis de Pedro Bandeira (1942), organiza a bibliografia completa das obras de Pedro Bandeira desde 1976 a 2010 e agrupa um conjunto de 50 entrevistas concedidas por este autor durante o período ora observado. As narrativas analisadas são: De piolho a garrote (1984); A marca de uma lágrima (1986); O fantástico mistério de Feiurinha (1986); Na colméia do inferno (1991), De punhos cerrados (2005) e Garrote, menino coragem (2009). Estas três últimas narrativas correspondem à reescrita do título de 1984. A análise descritiva destes quatro títulos que correspondem sempre a uma mesma história possibilitou a discussão das diferenças e semelhanças entre as quatro versões e permitiu levantar algumas das razões pelas quais nenhum deles obteve o sucesso de mercado e a crítica favorável alcançados pelos dois títulos lançados em 1986, apesar dos esforços do escritor para que isso ocorresse, reescrevendo continuamente a história original. A análise das narrativas foi realizada com base em conceitos de teóricos da literatura como: Gerard Genette, Claude Brémond, Algirdas Julien Greimas, Vladimir Iakovlevich Propp, Tzvetan Todorov, dentre outros / Abstract: The present study was categorized in two volumes which analyze six juvenile narratives of Pedro Bandeira (1942), organize a complete bibliography from Pedro Bandeira's work from 1976 to 2010, and group a pull of 50 interviews granted by this author during that same period of time. The scrutinized narratives were: De piolho a garrote (1984), A marca de uma lágrima (1986), O fantástico mistério de Feiurinha (1986), Na colméia do inferno (1991), De punhos cerrados (2005), and Garrote, menino coragem (2009). These last three narratives are a rewriting of the title from 1984. The descriptive analysis of these four titles, which always keep up a correspondence to the same story, enabled a discussion between differences and similarities among the four versions, and allowed to raise a few reasons why none of them achieved the market success or the favorable critique accomplished by the two titles released in 1986, despite the writer's effort for that to happen by rewriting the original story repeatedly. The narratives analyses were performed based on literature theoretical concepts such as: Gerard Genette, Claude Brémond, Algirdas Julien Greimas, Vladimir Iakovlevich Propp, Tzvetan Todorov, among others / Doutor

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / Campylobacter sp. à uma importante causa de enterite de origem alimentar com alta incidÃncia na populaÃÃo infantil de paÃses em desenvolvimento. No entanto, o diagnÃstico especÃfico de sua etiologia segue como um desafio, visto que mÃtodos moleculares e imunoenzimÃticos tÃm se mostrado mais sensÃveis. Postulamos que o conhecimento de sua virulÃncia e o diagnÃstico especÃfico possam ajudar na identificaÃÃo e potencial controle da campilobacteriose na infÃncia. Foi determinada a etiologia de diarreia por Campylobacter sp., em um estudo transversal sobre diarreia em crianÃas de 0-36 meses residentes da Ãrea urbana de Fortaleza, CearÃ, Brasil, que necessitaram de atendimento mÃdico de urgÃncia por causa de doenÃa diarreica. ApÃs a aprovaÃÃo Ãtica do estudo, um questionÃrio foi aplicado para qualificar as condiÃÃes clÃnicas apresentadas por cada crianÃa no momento da admissÃo. O DNA foi extraÃdo diretamente de amostras fecais coletadas de 226 crianÃas. Para a detecÃÃo do agente etiolÃgico, utilizamos diagnÃstico molecular (PCR e RT-PCR) e diagnÃstico imunoenzimÃtico (ELISA), alÃm da detecÃÃo de genes associados à virulÃncia de C. jejuni (PCR). Campylobacter sp. foi encontrado em 8,9% (20/225) das amostras, por diagnÃstico microbiolÃgico convencional. C. jejuni e C. coli foram detectados em 19,5% (44/226) e 1,3% (3/226) das amostras diarreicas, respectivamente, por PCR. Os diagnÃsticos por RT-PCR e ELISA alcanÃaram 26,7% (60/225) e 37,9% (58/153), respectivamente. Quando considerada a combinaÃÃo de diagnÃsticos (positividade no diagnÃstico microbiolÃgico ou no imunoenzimÃtico e ao menos em um dos testes moleculares) a prevalÃncia encontrada foi de 16,4% (37/226). A concordÃncia entre os testes para diagnÃstico utilizados foi de moderada a regular, de acordo com o Ãndice de Kappa. Genes associados à virulÃncia foram detectados nas seguintes proporÃÃes de amostras positivas para C. jejuni: flaA, 79,5% (35/44); racR, 97,7% (43/44) e dnaJ, 88,6% (39/44) â relacionados à adesÃo bacteriana e colonizaÃÃo; ciaB, 97,7% (43/44); pldA, 45,4% (20/44) e pVir 0% (0/44) â relacionados à invasÃo; e cdtABC em 95,4% (42/44) das amostras, operon relacionado à produÃÃo da toxina citoletal distensora (CDT). Sinais e sintomas especÃficos, tais como sangue nas fezes, vÃmito, febre e/ou dor abdominal, apesar de bastante frequentes, nÃo foram associados com a detecÃÃo de C. jejuni. O perfil de distribuiÃÃo dos genes de virulÃncia de C. jejuni nÃo apresentou correlaÃÃo com a apresentaÃÃo clÃnica da doenÃa, mesmo quando tal perfil foi categorizado de acordo com a funÃÃo das proteÃnas codificadas pelos genes, o que nos leva a crer que outros fatores, talvez relacionados à susceptibilidade do hospedeiro, possam ser mais importantes do que a variabilidade genÃtica do micro-organismo. ConcluÃmos que Campylobacter sp. foi detectado em percentual relevante da populaÃÃo estudada, principalmente quando os mÃtodos diagnÃsticos foram utilizados de forma combinada. Em geral, os genes de virulÃncia foram detectados em uma alta proporÃÃo das amostras positivas para C. jejuni, embora os genes relacionados à invasÃo tenham sido menos frequentemente encontrados. Corroboramos dados de outros grupos sobre a necessidade de revisÃo do diagnÃstico para Campylobacter sp. em prol da inclusÃo de metodologias mais sensÃveis e espÃcie-especÃficas, alÃm da busca por marcadores para inflamaÃÃo intestinal e fatores preditivos de cultura negativa. / Campylobacter sp. is an important cause of food-borne gastroenteritis with high incidence in children living in developing countries. However, the specific diagnosis of its etiology remains as a challenge, since conventional diagnosis by culture is now challenged by molecular and immunoenzymatic methods, which have greater sensitivity. We postulate that the knowledge of its virulence and specific diagnosis may assist in identifying and potentially controling campylobacteriosis in childhood. We determined the etiology of Campylobacter sp. associated diarrhea, in a cross-sectional study of diarrhea in children aged 0-36 months from the urban area of Fortaleza, CearÃ, Brazil, who required emergency medical care because of diarrheal disease. After ethical approval of the study, a questionnaire was applied to describe the clinical conditions presented by each child at the time of admission. DNA was extracted directly from fecal samples collected from 226 children. For the determination of the etiologic agent we used molecular diagnostics (PCR and RT-PCR) and diagnostic immunoassay (ELISA), besides the detection of virulence associated genes of C. jejuni (PCR). Campylobacter sp. was found in 8.9% (20/225) of the samples by conventional microbiological diagnosis. C. jejuni and C. coli were detected in 19.5% (44/226) and 1.3% (3/226) of the diarrheic samples, respectively. The diagnostic RT-PCR and ELISA reached 26.7% (60/225) and 37.9% (58/153) of positivity, respectively. When considering the combination of diagnostic (positive in microbiological diagnosis or immunoassay and at least one of the molecular tests) the prevalence was 16.4% (37/226). The agreement between the tests used for diagnosis was moderate to regular, according to Kappa index. The presence of C. jejuniÂs virulence-associated genes that encode proteins related to the pathogenesis of micro-organism were detected in the following proportions of C. jejuni-positive DNA samples: flaA, 79.5% (35/44); racR, 97.7% (43/44) and dnaJ, 88.6% (39/44) â related to bacterial adhesion and colonization; ciaB, 97.7 % (43/44); pldA, 45.4% (20/44) and pVir 0% (0/44) â related to invasion, and cdtABC in 95.4% (42/44) of samples related to citoletal distending toxin (CDT). Specific signs and symptoms such as blood in the stool, vomiting, fever and/or abdominal pain, although quite frequent, were not associated with the detection of C. jejuni. The distribution profile of C. jejuniÂs virulence genes was not correlated with the clinical presentation of the disease, even when this profile was categorized according to the function of the proteins encoded by the genes, which leads us to believe that other factors, perhaps related to host susceptibility, may be more important than genetic variability of the microorganism. We conclude that Campylobacter sp. was detected in a significant percentage of the children 0-36 months with diarrhea, especially when the diagnostic methods were used in combination. In general, the virulence genes were detected in a high proportion of C. jejuni-positive samples, although the invasion-related genes have been found less frequently. Our data corroborates findings from other groups on the need to revise the diagnostic for Campylobacter sp. towards the inclusion of more sensitive and species-specific methods, as well as search for extra markers for intestinal inflammation and predictors of negative culture.

Touchant près de 3 à 10 % des nouveau-nés, les hémangiomes infantiles (HI) sont des tumeurs vasculaires bénignes, les plus fréquentes chez les nourrissons. Les HI sévères sont actuellement traités par un bêtabloqueur, le propranolol, dont l’efficacité a été découverte de manière fortuite. Ainsi, son mécanisme d’action est méconnu. Le propranolol se fixe sur les récepteurs beta-adrénergiques et empêche leur activation par les catécholamines comme la noradrénaline. Nous nous sommes donc interrogés sur la relation entre le propranolol et la noradrénaline dans cette tumeur. Nous avons montré une forte expression de la noradrénaline et des enzymes de synthèse des catécholamines dans les HI, comparés aux hémangiomes congénitaux, qui diminuent lorsque la tumeur involue ou est traitée par le propranolol. Nous avons ensuite réalisé un modèle in vitro ressemblant à l’HI à partir de cellules isolées d’HI capables de synthétiser les catécholamines : les cellules endothéliales et les péricytes. Ce modèle nous permettra d’étudier l’impact de la noradrénaline et du propranolol sur ces cellules. Parallèlement, notre équipe a réalisé un modèle in vivo qui a permis de mettre en évidence le rôle clé de la protéine quaporine-1 (AQP1) dans la réponse antitumorale du propranolol. Nous avons également étudié l’expression de l’AQP1 dans les HI et les hémangiomes congénitaux et découvert un type cellulaire adventitiel exprimant l’AQP1 dans les HI, le télocyte. Au total, notre travail sur l’HI a mis en évidence d’une part une possibilité de production endogène accrue de noradrénaline, probablement antagonisée avec succès par le propranolol, et la découverte de télocytes AQP1+ qui pourraient avoir un rôle dans la spécificité de la réponse des HI au propranolol. / Affecting nearly 3 to 10 % of newborns, infantile hemangiomas (HI) are the most common benign vascular tumors in infants. Severe HIs are currently treated with a beta-blocker, propranolol, whose efficacy was discovered by serendipidity. Propranolol binds to beta-adrenergic receptors and prevents their activation by catecholamines such as noradrenaline. We therefore wondered about the relationship between propranolol and noradrenaline in this tumor. We showed a strong expression of noradrenaline and catecholamine synthesis enzymes in HI, compared to congenital hemangiomas, which decrease when the tumor involutes or is treated with propranolol. We then realize an in vitro model resembling HI from cells isolated from HI capable of synthesizing catecholamines: endothelial cells and pericytes. This model will permit to study the impact of noradrenaline and propranolol on these cells. At the same time, our team created an in vivo model that highlighted the key role of aquaporin-1 protein (AQP1) in the antitumor response to propranolol. We have also studied the expression of AQP1 in HI and congenital hemangiomas, and discovered an adventitious cell type expressing AQP1 in HI, the telocyte. Altogether, our work on HI has revealed firstly the possibility of increased endogenous production of norepinephrine, probably successfully antagonized by propranolol, and secondly the presence of AQP1 + cells which could have a central role central in the specificity of HI response to propranolol.

Diss. (sammanfattning) Stockholm : Karol. inst., 2005. / Härtill 5 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst., 2006. / På titelsidan felaktigt: immunotherpay. Härtill 7 uppsatser.

Si l’œuvre de Lyotard reste en suspens de son « supplément », qui serait comme « une ligne de résistance » dans la pensée, « l’insu qui hante sa philosophie », il semble bien que celui-ci serait une sorte de « phrase ‘infante’ ». Mais plus profondément, ce travail cherche à montrer que l’enfance est cet inconnu à multiples figures habitant l’œuvre de Lyotard bien avant le tournant du différend. Tissée par un mouvement dérivatif critique entre le langage, la psychanalyse et l’art, la pensée de Lyotard semble soucieuse de toucher la limite entre le système et l’événement, le même et l’autre, la structure et la différence, cette limite où l’hétérogène apparaît comme une force à la fois constitutive et refoulée. C’est justement ce que nous croyons repérer à travers les visages multiples de l’enfance. Ces visages qui ne se réduisent pas l’un à l’autre, se déclinent alors de l’infantile à la minorité et de là à l’infantia, Lyotard cherchant à toucher cet étranger chaque fois avec des instruments différents. Cette façon de lire Lyotard nous semble avoir l’avantage de mettre en relief certains topiques peu explorés de son œuvre, ou encore de voir sous un nouveau jour certains aspects surexploités de cette pensée. Ainsi l’on pourrait varier les travaux sur la pensée de Lyotard vers certains domaines inhabituels actuellement, comme la philosophie de l’éducation ou les études de la performance. / If Lyotard’s work is waiting for its « supplement » which would be like a « resistance line » in the thought, « the unbeknownst haunting his philosophy », it seems that this would be an « ‘infante’ phrase ». More thoroughly, we would like to show that childhood is a multiple figure inhabiting his work even before the turning of the différend. Lyotard’s thought, weaved in a critical derivative movement between language, psychoanalysis and art, seems to be attentive to the limit between the system and the event, the same and the other, the structure and the difference, that limit where the heterogenous appears to be a constitutif yet inhibited force. This is exactly what we hope to map out across the multiple faces of the childhood. Those faces not reducing to each other, vary from the infantile to the minority and from the minority to infantia. Thus Lyotard tries to touch this stranger with an other instrument each time. This way of reading Lyotard seems to have the advantage of highlighting some unexplored topics of his work, or to bring a new light on its over exploited aspects. Thereby we could vary works on Lyotard’s philosophy towards actually unfamiliar domains with it, like the philosophy of education or performance studies

Diarrhoeal disease is a major cause of infant mortality in this and other developing countries. The assessment of the degree of dehydration in these children is often based on subjective findings alone. These have been shown to be inaccurate as an assessment of the degree of dehydration. Whole-body impedance (WBI) is a method of measuring total body water which is used to assess body composition. This WBI technology, which operates at a single frequency, has been applied to the assessment of dehydration in children with diarrhoeal disease. The normal range of WBI was determined on a group of normally hydrated children and was found to have a mean of 746 Ω, a standard deviation of 85 Ω and 95 % confidence interval for the mean from 720 Ω to 772 Ω. WBI did not depend on age, mass, height or sex. The WBI of a group of children dehydrated from infantile gastroenteritis was measured both before and after rehydration. The dehydrated group had a mean WBI on admission of 1089 Ω and a standard deviation of 149 Ω with a 95 % confidence interval for the mean from 950 Ω to 1109 Ω. This value was significantly greater than that of the normal group. The WBI of the dehydrated group after rehydration was not significantly different from the normal group. In addition a variable frequency bioimpedance analyser was designed and tested. It was found to have an error of less than 1 % over the frequency range 1 to 100 kHz. The device was evaluated on 11 normal and dehydrated children. Four dehydrated children were tested before and after rehydration and this data was compared to a standard electrical model for WBI. It was found that the model could represent the measured data over this frequency range. The extracellular resistive element of the model was mainly responsible for the changes seen during rehydration, suggesting that dehydration in gastroenteritis is mainly due to fluid loss from the ECF compartment.

L'hémangiome capillaire infantile (IH) est une tumeur vasculaire bénigne courante. Les formes sévères sont traitées depuis 2008 par propranolol bien que le mécanisme d’action reste à ce jour inexpliqué. La 1ère partie de ma thèse est une étude de tolérance chez les enfants traités par propranolol dans le cadre de l'ATU française. L'analyse de cette base de données prospective de 922 enfants a permis de confirmer la bonne tolérance du propranolol dans la population pédiatrique, et de cibler les situations à risque de complication. La 2e partie est une étude de la signalisation adrénergique à partir des tissus d'IH opérés. Tout d'abord, nous avons identifié par immunofluorescence les cellules exprimant les récepteurs béta-adrénergiques ADRB1, 2 et 3 : Nous avons observé une forte expression d'ADRB2 sur le mastocyte, et de manière plus modérée d'ADRB1 et 2 sur les vaisseaux. Ce profil d'expression était retrouvé quel que soit le degré d'involution de la tumeur, et était également observé sur des tumeurs vasculaires contrôles qui elles ne répondent pas au propranolol. Nous avons donc envisagé une activation des récepteurs ADRB propre aux IH prolifératifs. Cette hypothèse a été confirmée par la mise en évidence, de l'expression d'enzymes de synthèse des catécholamines et de marqueurs neuroendocrines sur les péricytes des IH prolifératifs, sur tissus inclus en paraffine et également péricytes d’IH mis en culture. La surexpression de HIF1-a sur ces cellules a conduit à tester l’effet du propranolol en hypoxie. Nous n’avons pas mis en évidence d'effet sur la prolifération cellulaire, mais par contre une inhibition de la sécrétion de VEGF induite par l’hypoxie. / Capillary infantile haemangioma (IH) is a common benign vascular tumour of infants. Severe forms are treated since 2008 by propranolol. However, its dramatic efficacy remains until now unexplained.The first part of my thesis is a safety study of children treated with propranolol as part of the French Compassionate Use Program. The prospective vigilance database, including 922 children, confirmed the safety of propranolol among the paediatric patients, and highlighted the circumstances at risk of complications.The second part is a study of adrenergic signaling in IH tissues. First, we identified by immunofluorescence the expression of beta-adrenergic receptors on IH cells. We observed a strong expression of ADRB2 on mast cells, and a moderate ADRB1 and 2 expression on vessels. This expression pattern was the same regardless of the degree of involution of the tumour, and has also been observed on tumour vascular controls which did not respond to propranolol. We therefore hypothesized that ADRB receptors may be activated in proliferative IH. We indeed detected the expression of catecholamine synthesis enzymes and of neuro-endocrine markers on pericytes in paraffin-embedded tissues, and also in IH pericytes in culture. We also detected HIF1-alpha overexpression and therefore explored propranolol effect during hypoxia. Propranolol had no effect on cell proliferation, but reduced hypoxia-induced secretion of VEGF-A.

No description available.