The evolution of novel protein functions and protein families is a fundamental question within both evolutionary biology and biochemistry. While many gene families follow predictable patterns of molecular tinkering, many protein families exist with completely novel functions now essential. The guanylate kinase protein interaction domain (GKPID) of the membrane associated guanylate kinases (MAGUK) represents a model system for the study of protein evolution in which a protein scaffolding domain has evolved from a nucleotide kinase ancestor. Here we elucidate the ancient mechanisms by which these new functions evolved by combining ancestral protein reconstruction with in vitro and cell-biological molecular experiments. We found that the GKPID's capacity to serve as a mitotic spindle-orienting scaffold evolved by duplication and divergence of an ancient guanylate kinase enzyme before the divergence of animals and choanoflagellates. Re-introducing a single historical substitution into the ancestral guanylate kinase is sufficient to abolish the ancestral enzyme activity, confer the derived scaffolding function, and establish the capacity to mediate spindle orientation in cultured cells. This substitution appears to have revealed a latent protein-binding site, rather than constructing a novel interaction interface, apparently by altering the dynamics or conformational occupancy of a hinge region that determines whether the binding site is exposed or hidden. Three further substitutions also conveyed a measure of ligand specificity to phosphorylated Pins, which is necessary in metazoan spindle orientation pathways. These findings show how a small number of simple, ancient genetic changes caused the evolution of novel molecular functions crucial for the evolution of complex animals and laid the groundwork for an entirely new family of metazoan scaffolding proteins. This dissertation contains previously unpublished, co-authored material.

Further analyses of comparing the creativity scores of the experimental groups also showed inconsistent results. Participants in the promotion cue condition scored significantly higher in uniqueness and unusualness of standardized assessment, and fluency and flexibility of creative problem solving than those in the prevention cue condition. The correlation between promotion focus and creativity was found significantly higher under the promotion cue condition than the prevention cue condition. But other mixed results showed that the control group scored significantly higher fluency and flexibility of standardized assessment than participants in the promotion cue condition. Participants' levels of promotion focus trait also had main effects on their creativity scores. Participants with higher trait of promotion focus tended to score higher in most of the creativity indicators. / In conclusion, the present study provided some limited evidence that promotion focus as a personality trait had weak but positive associations with creativity, and achievement goal as a weak mediator in affecting students' creativity. Students' promotion trait had stronger main effect on their creative performance than situational promotion focus. Ways to improve the manipulation effects and future direction on research had been suggested. / Results showed that the manipulation was relatively ineffective as reflected by the manipulation questions. Participants' promotion trait had, however, main effects on the manipulation items. Participants with high promotion trait scored significantly higher in all the checking items of promotion focus. Participants' prevention trait also had main effects on their perception of checking items of prevention focus. Participants with high prevention trait got higher scores of prevention situation than individuals with medium and low trait level. / Study 2 took a further step to examine the effects of regulatory focus on creativity in 490 Grade 7 students who had taken part in the previous study. Participants of 13 intact classes were randomly assigned to one of the conditions, namely promotion-framing condition (188 students from 5 classes), prevention-framing condition (149 students from 4 classes), and the control condition (153 students from 4 classes). Verbal and written instructions were provided with an intention to frame the regulatory focus of individual students. Participants completed a parallel item of the alternate use battery of the standardized creativity assessment, another item of creative problem solving, and six checking questions. Previous scores of achievement goals and regulatory foci were used as covariates in analysis of variance to examine the differences in the checking items. / The present study aimed at examining the influence of two motivational variables---achievement goal and regulatory focus on creativity. It was composed of two empirical studies. Study 1 was designed to investigate how achievement goal and regulatory focus affected creativity. A total of 824 Grade 7 students took part in completing a questionnaire which comprised an alternate use item of a standardized creativity assessment, a creative problem solving task, an instrument on achievement goal and two instruments on regulatory focus. Exploratory factor analyses and confirmatory factor analyses were conducted to confirm the factor structures of achievement goals (mastery-approach goal, performance-approach goal, mastery-avoidance goal, and performance-avoidance goal) and regulatory foci (promotion focus and prevention focus). Results showed that these two variables were basically distinctive in nature. Positive but weak correlations were found between promotion focus and creativity, as well as prevention focus and creativity. Positive and weak correlations were also found between mastery-approach goal and creativity, performance-approach goal and creativity, as well as mastery-avoidance goal and creativity. Promotion focus had moderately high associations with both approach goals (mastery-approach and performance-approach) and avoidance goals (mastery-avoidance and performance-avoidance), and so had prevention focus. Regression analyses indicated that the strongest predictor for creativity was promotion focus. Performance-approach goal and mastery-avoidance goal served as weak mediators of regulatory focus on creativity. / Hui Na Na Anna. / "November 2006." / Adviser: P. C. Cheung. / Source: Dissertation Abstracts International, Volume: 68-08, Section: A, page: 3279. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (p. 206-224). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract in English and Chinese. / School code: 1307.

by Leo Li Kai-chung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Bibliography: leaves 55-56. / Chapter Chapter One --- Introduction --- p.2 / Chapter Chapter Two --- Analytical Framework / Chapter (1) --- Conceptual Review --- p.5 / Chapter (2) --- Theoretical Relevance Individual Differences in Language Learning --- p.8 / Chapter (3) --- The Studies of Lambert and Gardner Integrative vs. Instrumental Orientation of Second Language Learning --- p.12 / Chapter (4) --- Anomalies Arising Out of Language Learning Attitude Studies Challenge To Lambert's & Gardner's Orientation Theories --- p.17 / Chapter (5) --- Politzer's Learning Behaviour Studies Alternative Explanation To Language Learning Proficiency --- p.23 / Chapter Chapter Three --- Hypothesis & Data Collection Method --- p.28 / Chapter Chapter Four --- Findings & Discussion / Chapter (A) --- Findings --- p.37 / Table 1: Personal Data & Background of English Learning Experience of Subjects --- p.39 / Table 2: Orientation by Proficiency --- p.43 / Table 3: Orientation & Proficiency by Low Language Learning Behaviour --- p.44 / Table 4: Orientation & Proficiency by High Language Learning Behaviour --- p.44 / Chapter (B) --- Discussion --- p.45 / Bibliography --- p.55 / Appendix --- p.57 / Questionnaires

by Li Shing Sun. / Thesis (M.A.Ed.)--Chinese University of Hong Kong, 1987. / Bibliography: leaves 111-113.

This study investigates how the subsidiaries of multinational enterprises (MNEs) reconfigure their resource bases to respond to an altered strategic positioning. In particular, the focus is on the subsidiaries of multinational enterprises from emerging economies (EMNEs), which undertake transformation from export-orientation to host market-orientation in an emerging economy being host country. Two Taiwanese MNEs with subsidiaries in China extensively operated the host market are selected as case studies. This research is grounded in a preliminary conceptualisation covering three main areas: subsidiary organisations, external actors in the host country and headquarters’ functions. It provides insights into (1) how the subsidiaries align their historical resources with newly-developed capabilities; (2) how the subsidiaries govern inter-firm relations with external actors in the host environment; and (3) whether and how complementary resources are to a limited extent transferred from headquarters to subsidiaries so as to support the operations in the host market. With regards to the organisational initiatives undertaken by the case subsidiaries, it is found that the historical resources are leveraged to support the host market business, whilst at the same time developing required marketing capabilities. In addition, the concept of organisational ambidexterity is adopted to refer to way in which existing (export-oriented) and new (host market-oriented) businesses that are operating simultaneously. As to the inter-firm relations, the case subsidiaries have been mainly governing their relations with distributors by performance-based mechanisms aiming to secure stable profits. By contrast, the relation-based trust was not commonly observed among the cooperation ties. With growing brand strengths and increased direct contact with consumers, they held higher authority over the interfirm relations with distributors than during the initial stages of operating in the Chinese market. In addition, with regards to the cross-border resource transfers, two distinct modes of headquarters-subsidiary relationships emerged: a traditional one, in which headquarters allocate resources within MNEs and a novel one in which headquarters’ functions were gradually replaced by the powerful subsidiary, termed in this thesis as “migrating headquarters”. On the basis of these findings, we put forward a set of propositions that present the interrelations between the resource circumstances of case subsidiaries, the institutional environments and the organisational initiatives undertaken by the case subsidiaries. Theoretically, the contributions of this study are threefold. Firstly, it advances the research on subsidiary development by holistically exploring the: resource reconfiguration of subsidiaries, inter-firm relations with external actors and headquarters-subsidiary relationships. In particular, the resource deficiency which the EMNEs’ subsidiaries encountered and the characteristics of the required capabilities for the host market-oriented transformation, i.e. local marketing competences, were investigated. Secondly, through probing the governance mechanisms adopted regarding interfirm relations between the case subsidiaries and local distributors, this study not only addresses the question of how MNEs acquire this location-bound resource, but also advances the extant research by the aspect of network positions. That is, this study indicates that the first-tier distributors hold more relations-based interactions with the case subsidiaries than the lower-tier ones did. Moreover, unlike the reliance on informal relations suggested by extant literature on doing business in emerging economies, it is found that the economic governance mechanisms based on distributor performance have been predominantly adopted by the case subsidiaries. Thirdly, by investigating how the complementary resources are transferred to the subsidiaries, this work discovers EMNEs’ weakness at responding to the host market-oriented subsidiary transformation in terms of resource deployment within MNEs, in particular those resources that have been mostly controlled by headquarters. In addition, the term “migrating headquarters”, which represents an extreme outcome of subsidiary development, provides novel knowledge to the extant literature on the relocation of MNE headquarters by the perspective of resource circumstances. Moreover, the five components comprising dynamic capabilities in the context of subsidiary transformation are identified through the two case studies as being: capability upgrading, capability leverage, capability building, coordination capability and cooperative capability.

Creative Journeys contribute to our knowledge of how practical ontology navigates multi-perspectives through an auto-ethnographic journey with material. I investigate how it may be possible to navigate geographic locations – Norway, Britain and Spain – through knitting as an approach to practical and philosophical exploration. In Creative Journeys I am in a process of reflexive practice, engaged in external and internal dialogue, haptic encounters, challenges and creative action. My thesis suggests that engagement with material is a fluid process and understanding evolves, so too does my journey in life. In such circumstances material functions as a mediator; creates a bridge between hand, movement, time and space. Material transcends boundaries, assists orientation and facilitates articulation of aesthetics, reminiscence, symbols, patterns, colour, sensory appreciation; all of which contribute to an understanding of relationships. Body is material and being conscious of body movement with the rhythm of diverse locations enables me to make connections through daily events, to attune to different atmospheres. In such a journey there are moments of harmony and misunderstanding, discord and adjustments; interruptions occur with energy and disrupt patterns of life. These are crossing points which enable me to experience myself through the perspective of the other; to understand how situated knowledge changes in relation to diverse perspectives; and to understand how I may contribute to the social fabric of life of diverse locations through the art of paying attention to detail. Creative Journeys are investigated through three questions: How do I relate to the world? How do art subjectivities manifest themselves through art practice? How does art evolve through relations? The questions are examined within the perspective of situated knowledge; subjectivities; material of location and practice. Investigating material in the context of these questions provides opportunities to develop capacities to navigate social, cultural and political orientation, economy, health, race, gender and belief, which all impact on the journey. My approach to the thesis evolved through my relations with creative works of knitted artefacts which I documented in personal journals. The components of practice have woven threads of inquiry through theory and reflective critical practice and form an aspect of the viva voce examination. Along with the illustrations they contribute to 20% of the written component of the thesis.

Thesis (Ed.M.)--Boston University / The purpose of this study is to attempt to determine the effect of different types of leadership for freshman orientation groups at Boston University School of Education

Notre travail de recherche a consisté à examiner « comment et de quelle manière le SI contribue-t-il à la performance organisationnelle » et à vérifier si cette contribution diffère selon l’orientation stratégique de l’entreprise. Nous nous positionnons dans le courant de recherche de l’évaluation en nous basant sur la théorie de ressources, l’approche processuelle et le modèle ISSM. Les responsables de 102 entreprises industrielles tunisiennes ont évalué les actifs de leur SI ainsi que leurs contributions aux processus et à la performance individuelle et organisationnelle. Des outils de mesure ont été élaborés et validés : qualité du SI, valeur d’usage du SI. Notre approche processuelle en quatre étapes assure une bonne explication de nos construits latents : qualité du SI, valeur d’usage du SI, contribution du SI à la performance individuelle et organisationnelle, performance concurrentielle et taux de croissance moyen du chiffre d’affaires. Notre modèle structurel explique 12 % du taux de croissance du chiffre d’affaires avec un GoF de 54 %. Les relations entre l’orientation stratégique, le SI et la performance, soulèvent plusieurs dilemmes auxquels des recherches futures devraient pouvoir répondre. / Our research consists in examining "how IS contributes to organizational performance" and whether this contribution differs depending on the strategic orientation of the firm.We position ourselves in the field of research assessment based on the theory of resources, process-based approach and the ISSM model. Officials of 102 industrial firms Tunisian have valued the assets IS and their contributions to the process and the individual and organizational performance.Measurement tools have been developed and validated: IS quality, use value of SI. Our four-step process approach provides a good explanation of our latent constructs: quality of IS , use value of IS , IS contribution to individual and organizational performance, competitive performance and average growth rate of sales. Our structural model explains 12% of the growth rate of sales with GoF 54%. The relationships between strategic orientation, IS and performance, raise several dilemmas for which future research should be able to respond.

The purpose of this dissertation was to create a psychometrically sound measure of asexual prejudice through microaggressions that can be used to document and identify the unique experiences of asexual people (i.e., those reporting a lack of sexual attraction towards others). Asexual prejudice encompasses anti-asexual beliefs and attitudes that stem from sexual normativity which promotes sexuality as the norm while positioning asexuality as deviant (Carrigan 2011; Chasin, 2011; Flore, 2014; Gupta, 2013). Applying Sue’s (2010) description of microaggressions, asexual microaggressions are conscious and/or unconscious daily occurrences of insults and invalidation that stem from implicit bias against asexual people and asexuality. Development of the scale included creating items with content that was derived from close readings of the literature on asexuality and related measures of discrimination, prejudice or bias as well as expert review for clarity and verifying applicability of content. A total of 738 participants participated on-line and half were randomly assigned to Phase 1 for the Exploratory Factor Analysis (EFA) while the other half was assigned to Phase 2 for the Confirmatory Factor Analysis (CFA). Results of the EFA indicate a 16 item four-factor structure for the AMS that capture expectations of sexuality, denial of legitimacy, harmful visibility, and assumptions of causality as descriptors of the types of microaggressions that occur. The CFA revealed support for the AMS total score with good internal consistency and strong validity as reflected in strong positive relationships with stigma consciousness, collective self-esteem, and another measure of discrimination and bias. Combined, the AMS is a valid and reliable measure of asexual prejudice. Contextualization of these results as well as implications for future research and clinical practice are discussed.

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