[pt] O escoamento anular é caracterizado por um núcleo gasoso fluindo a alta velocidade com um filme líquido no seu entorno, molhando a parede do duto. A presença de gotículas líquidas no núcleo gasoso resulta em impacto relevante em características do escoamento anular, como gradiente de pressão e propriedades das ondas presentes no filme líquido. A formação de gotículas se dá usualmente na crista das ondas de perturbação presentes na interface líquido-gás. No presente trabalho, é realizado um estudo do regime anular com presença de gotículas em tubulações verticais utilizando o Modelo de Dois Fluidos unidimensional. Um modelo de transferência de massa das gotículas é desenvolvido e acoplado ao modelo de Dois Fluidos. O modelo resultante permite capturar a evolução automática da interface gás-líquido e a formação de ondas de filme líquido e sua influência no desprendimento e deposição de gotículas. Analisa-se o desempenho de três modelos de entranhamento de gotículas disponíveis na literatura, além de um modelo de deposição de gotículas. Considerando que gotículas são criadas por cisalhamento nas cristas das ondas de perturbação, modificações dos modelos são propostas com a finalidade de melhor capturar a influência das ondas do filme líquido nos processos de entranhamento e deposição de gotículas. Parâmetros do escoamento como gradiente de pressão, espessura do filme do líquido e variáveis relacionadas com as ondas interfaciais são avaliados, mostrando boa concordância com dados experimentais disponíveis na literatura. / [en] Annular flow is characterized by a high velocity gas core flow, with a thin liquid film around it, wetting the pipe wall. The presence of liquid droplets in the gas core has relevant impact on annular flow characteristics, such as pressure drop and liquid film wave properties. Droplets are usually created by shear at disturbance wave crests, along the gas-liquid interface. At the present work, vertical annular flow with droplet entrainment is studied using the 1-D Two-Fluid model. A droplet mass transfer model is developed and coupled to the Two-Fluid model. The resulting model allows capturing the automatic evolution of the gas-liquid interface, liquid film wave formation and the waves influence on droplet entrainment and deposition. A performance analysis in carried out for three droplet entrainment models available in literature, as well as one deposition model. Taking into account that droplets are created by disturbance wave crest shearing, model modifications are proposed, aiming to better capture the influence of liquid film waves on droplet entrainment and deposition mechanisms. Flow parameters such as pressure drop, film thickness and wave features are evaluated, showing good agreement with experimental data found in literature.

The deep learning-based automatic segmentation methods have developed rapidly in recent years to give a promising performance in the medical image segmentation tasks, which provide clinical medicine with an accurate and fast computer-aided diagnosis method. Generative adversarial networks and their extended frameworks have achieved encouraging results on image-to-image translation problems. In this report, the proposed hybrid network combined cycle-consistent adversarial networks, which transformed contrast-enhanced images from computed tomography angiography to the conventional low-contrast CT scans, with the segmentation network and trained them simultaneously in an end-to-end manner. The trained segmentation network was tested on the non-contrast-enhanced CT images. The synthetic process and the segmentation process were also implemented in a two-stage manner. The two-stage process achieved a higher Dice similarity coefficient than the baseline U-Net did on test data, but the proposed hybrid network did not outperform the baseline due to the field of view difference between the two training data sets.

No description available.

This master's thesis explores the role of bespoke air handling units in enhancing energy efficiency in existing buildings. The context for the study is set against the backdrop of global initiatives, including the United Nations' Sustainable Development Goals, specifically Goal 7, which emphasizes the need to improve energy efficiency to combat climate change. The significance of enhancing energy efficiency is well-established, evident both at the EU level and in national policies and regulations. Buildings represent a significant portion of the energy utilization puzzle, with substantial potential for enhancing energy efficiency, although it is often underutilized. One of the contributing factors to inefficiency is outdated ventilation systems, which lead to high thermal losses. This challenge can be addressed by retrofitting these systems with modern, efficient air handling units, thus contributing to energy conservation and cost savings. This study focuses on the adoption of bespoke air handling units adjusted to the site and capable of accommodating constraints related to factors such as space limitations in machine rooms, existing ductwork layouts, and the location of shafts. The primary goal is to empower engineers to move beyond conventional approaches, enabling them to optimize technology choices based on local conditions, specific system performance requirements, and the economic viability of each project. The aim of this study is twofold: first, to develop a methodology for designing bespoke air handling units; and second, to demonstrate the practical application of this methodology in the context of two distinct renovation projects. In line with the aim of the thesis, a design methodology for site-tailored units equipped with a two stage flat crossflow heat exchanger and an indirect evaporative cooling system was developed. The methodology delves into different aspects of data analysis, 3D modeling, and the conduct of performance calculations.The established methodology was applied in two reconstruction projects in central Stockholm, where bespoke air handling units were designed in compliance with provided technical specifications. In both scenarios, a viable option emerged for accommodating a tailored unit within the technical room situated on the first floor. For both units, the energy performance metrics signify a notable achievement in terms of heat recovery efficiency, coupled with relatively modest requirements for heating and cooling power capacity from the combined heating and cooling aircoil. However, the calculated maximum specific fan power for a single unit with heat recovery exceeded the stipulated value specified in the technical specifications, which was accepted by the client. The results of the study included air handling unit product drawings, ventilation blueprints of the technical room with the integrated air handling unit, component specifications, unit flowcharts, performance calculations, and control operating pictures. The results of this work indicate that the improvement of the building's energy efficiency is rendered feasible through the installation of bespoke air handling units in the studied reconstruction projects.

With the rapidly growing need for long-term decision making in the presence of stochastic future events, it is important to devise novel mathematical optimization tools and develop computationally efficient solution approaches for solving them. Two-stage stochastic programming is one of the powerful modeling tools that allows probabilistic data parameters in mixed integer programming, a well-known tool for optimization modeling with deterministic input data. However, akin to the mixed integer programs, these stochastic models are theoretically intractable and computationally challenging to solve because of the presence of integer variables. This dissertation focuses on theory, algorithms and applications of two-stage stochastic mixed integer (non)linear programs and it has three-pronged plan. In the first direction, we study two-stage stochastic p-order conic mixed integer programs (TSS-CMIPs) with p-order conic terms in the second-stage objectives. We develop so called scenario-based (non)linear cuts which are added to the deterministic equivalent of TSS-CMIPs (a large-scale deterministic conic mixed integer program). We provide conditions under which these cuts are sufficient to relax the integrality restrictions on the second-stage integer variables without impacting the integrality of the optimal solution of the TSS-CMIP. We also introduce a multi-module capacitated stochastic facility location problem and TSS-CMIPs with structured CMIPs in the second stage to demonstrate the significance of the foregoing results for solving these problems. In the second direction, we propose risk-neutral and risk-averse two-stage stochastic mixed integer linear programs for load shed recovery with uncertain renewable generation and demand. The models are implemented using a scenario-based approach where the objective is to maximize load shed recovery in the bulk transmission network by switching transmission lines and performing other corrective actions (e.g. generator re-dispatch) after the topology is modified. Experiments highlight how the proposed approach can serve as an offline contingency analysis tool, and how this method aids self-healing by recovering more load shedding. In the third direction, we develop a dual decomposition approach for solving two-stage stochastic quadratically constrained quadratic mixed integer programs. We also create a new module for an open-source package DSP (Decomposition for Structured Programming) to solve this problem. We evaluate the effectiveness of this module and our approach by solving a stochastic quadratic facility location problem. / Doctor of Philosophy / With the rapidly growing need for long-term decision making in the presence of stochastic future events, it is important to devise novel mathematical optimization tools and develop computationally efficient solution approaches for solving them. Two-stage stochastic programming is one of the powerful modeling tools that allows two-stages of decision making where the first-stage strategic decisions (such as deciding the locations of facilities or topology of a power transmission network) are taken before the realization of uncertainty, and the second-stage operational decisions (such as transportation decisions between customers and facilities or power flow in the transmission network) are taken in response to the first-stage decision and a realization of the uncertain (demand) data. This modeling tool is gaining wide acceptance because of its applications in healthcare, power systems, wildfire planning, logistics, and chemical industries, among others. Though intriguing, two-stage stochastic programs are computationally challenging. Therefore, it is crucial to develop theoretical results and computationally efficient algorithms, so that these models for real-world applied problems can be solved in a realistic time frame. In this dissertation, we consider two-stage stochastic mixed integer (non)linear programs, provide theoretical and algorithmic results for them, and introduce their applications in logistics and power systems. First, we consider a two-stage stochastic mixed integer program with p-order conic terms in the objective that has applications in facility location problem, power system, portfolio optimization, and many more. We provide a so-called second-stage convexification technique which greatly reduces the computational time to solve a facility location problem, in comparison to solving it directly with a state-of-the-art solver, CPLEX, with its default settings. Second, we introduce risk-averse and risk-neutral two-stage stochastic models to deal with uncertainties in power systems, as well as the risk preference of decision makers. We leverage the inherent flexibility of the bulk transmission network through the systematic switching of transmission lines in/out of service while accounting for uncertainty in generation and demand during an emergency. We provide abundant computational experiments to quantify our proposed models, and justify how the proposed approach can serve as an offline contingency analysis tool. Third, we develop a new solution approach for two-stage stochastic mixed integer programs with quadratic terms in the objective function and constraints and implement it as a new module for an open-source package DSP We perform computational experiments on a stochastic quadratic facility location problem to evaluate the performance of this module.

El presente trabajo estudia el impacto de la educación de la madre sobre la desnutrición crónica infantil para los años 2002 al 2016 en Perú. Este estudio se realizó mediante un análisis por diversos métodos del modelo econométrico de Variables Instrumentales. Se presentan los resultados para Mínimos Cuadrados en dos Etapas y Método Generalizado del Momento. También se realizan los modelos econométricos de Inclusión Residual en Dos Etapas y Probit para datos de panel. En la presente investigación se utilizó la Base de Datos Niños del Milenio. Se obtiene como resultado principal que la educación de la madre tiene un impacto negativo sobre la desnutrición crónica infantil por cada modelo econométrico para los años 2002 al 2016 en Perú. Palabras clave: Mínimos Cuadrados en dos Etapas; Método Generalizado del Momento; Modelo de Inclusión Residual en Dos Etapas; Probit Panel; Salud; Desnutrición Crónica Infantil; Riqueza; Logro Educativo; Perú; Niños del Milenio. / The document studies the impact of maternal education on stunted children from 2002 to 2016 in Peru. It is analyzed by two different Instrumental Variables Methods, Two-Stage Least Squares and General Method of Moments. Also, the document uses Two-Stage Residual Inclusion Model, and Dynamic Probit Model for panel data. The present investigation uses the Young Lives data base. The main result is that the mother's education has a negative impact on stunted children for each model from 2002 to 2016 in Peru. Keywords: Two-Stage Least Squares; General Method of Moments; Two-Stage Residual Inclusion; Panel Probit; Health; Wealth; Stunting; Education Attainment; Young Lives; Peru / Tesis

Engine mapping is the process of empirically modelling engine behaviour as a function of adjustable engine parameters, predicting the output of the engine. The aim is to calibrate the electronic engine controller to meet decreasing emission requirements and increasing fuel economy demands. Modern engines have an increasing number of control parameters that are having a dramatic impact on time and e ort required to obtain optimal engine calibrations. These are further complicated due to transient engine operating mode. A new model-based transient calibration method has been built on the application of hierarchical statistical modelling methods, and analysis of repeated experiments for the application of engine mapping. The methodology is based on two-stage regression approach, which organise the engine data for the mapping process in sweeps. The introduction of time-dependent covariates in the hierarchy of the modelling led to the development of a new approach for the problem of transient engine calibration. This new approach for transient engine modelling is analysed using a small designed data set for a throttle body inferred air ow phenomenon. The data collection for the model was performed on a transient engine test bed as a part of this work, with sophisticated software and hardware installed on it. Models and their associated experimental design protocols have been identi ed that permits the models capable of accurately predicting the desired response features over the whole region of operability. Further, during the course of the work, the utility of multi-layer perceptron (MLP) neural network based model for the multi-covariate case has been demonstrated. The MLP neural network performs slightly better than the radial basis function (RBF) model. The basis of this comparison is made on assessing relevant model selection criteria, as well as internal and external validation ts. Finally, the general ability of the model was demonstrated through the implementation of this methodology for use in the calibration process, for populating the electronic engine control module lookup tables.

Cette thèse présente trois chapitres qui mobilisent un cadre d’analyse évolutionniste et qui étudient empiriquement la relation « innovation-performance » à partir de données CIS. Nous souhaitons montrer que les sources de l’innovation et les méthodes d’appropriation varient en fonction des secteurs d’activité et des stratégies d’innovation des firmes. Dans un premier temps, nous décrivons les tendances de l’innovation à partir de quatre vagues d’enquêtes CIS (1994-2006) et nous analysons la persistance de l’innovation sur un échantillon de 431 firmes avec une régression logistique binaire. Nous montrons que la persistance de l’innovation est plus élevée pour les firmes qui innovent en produits car ces firmes sont contraintes d’investir de manière continue dans des projets d’innovation pour rester compétitives. Les firmes qui innovent en procédés sont moins persistantes car leur stratégie est plus orientée vers des ajustements de la qualité des produits ainsi que vers l’amélioration des processus de production. Les deux derniers essais explorent avec la méthode des doubles moindres carrés le lien innovation-performance économique sur un échantillon de 7 742 firmes portant sur la période 2002-2005. Nous expliquons que la source principale de l’innovation des firmes à « forte intensité scientifique » est la R&D, d’une part, et que les méthodes d’appropriation des rentes de l’innovation passent par l’acquisition d’actifs complémentaires (tels que l’utilisation combinée de titres de propriété intellectuelle et de secrets de fabrication), d’autre part. En revanche, les firmes dans les autres catégories (notamment celles à fortes économies d’échelle) fondent leurs avancées technologiques sur des sources externes de l’innovation telles que les concurrents, les fournisseurs et les utilisateurs avancés. De plus, ces firmes utilisent de manière plus importante des méthodes d’appropriation commerciale telles que les marques ou les stratégies marketing, car leurs produits sont moins exposés au risque d’imitation certes, mais aussi parce qu’elles sont sensibles aux changements de coûts. / This thesis is structured in three essays based on evolutionary theoretical grounds and provides empirical evidence from CIS. It aims at showing that the sources of innovation and the appropriation of innovation rents vary in function of firms’ activities and innovation strategies.In essay 1, we describe four waves of CIS, covering the period 1994-2006 and we study persistent innovation behavior with a discrete choice model on a data set of 431 firms. We find that innovation persistence is more important for product innovators because they need novel products to be more competitive and therefore enrich their base of knowledge continuously. By contrast, process innovators are less persistent because innovation strategy is less “market” oriented and intends to meet quality or production adjustments. The two last essays explore with the two stage least squares method how firms benefit economically from their innovations on a sample of 7 742 firms, on the period 2002-2005. We show that science-based firms rely more on R&D investments to develop their products and maintain their leads by acquiring complementary assets, i.e. they use mixed methods to appropriate the rents of innovation (the combined use IPRs and strategic methods for instance secrecy). By contrast, firms in other categories (for instance firms using cost-cutting strategies) draw more on external sources of knowledge coming either from suppliers or advanced users. Additionally, these firms use more extensively trademarks or non technological methods of appropriation (as marketing devices), because they are less exposed to potential imitation and because they are price sensitive.

A digestão anaeróbia tem se apresentado como um processo de grande interesse sob a ótica da potencial produção de energia renovável (H2 e CH4), considerando-se a ampla variedade de compostos orgânicos que podem ser utilizados. Neste estudo desejou-se avançar na compreensão do sistema de reatores anaeróbios de duas fases (acidogênico seguido de metanogênico) operados em condições termofílicas (55°C), alimentados com melaço da cana-de-açúcar, subproduto da indústria sucroalcooleira. Os experimentos foram conduzidos em reatores anaeróbios de leito fixo estruturado com fluxo ascendente e o melaço foi diluído com água de abastecimento, para adequação da concentração aos processos de tratamento de águas residuárias. Na 1ª Etapa dois reatores acidogênicos foram operados em paralelo para avaliar diferentes formas de inoculação e meios suportes, a fim de manter a produção continua e estável de hidrogênio. Para isso foram aplicadas diferentes cargas orgânicas (2,5, 5 e 10 gDQO.L-1) que resultam em COV de 30, 60 e 120 g.DQO.Lreator1.dia-1, com TDH fixo de 2 horas. A expressão do gene hidrogenase foi detectado em ambos os reatores, mas em maior proporção no reator inoculado com lodo de reator UASB e usando como material suporte a espuma de poliuretano. Sequencialmente a este reator, foi acoplado um reator metanogênico, alimentado com efluente do reator acidogênico, estabilizado nas condições apresentadas, e operado com COV crescentes de 1, 2, 5, 7, 14, 17 e 26,5 gDQO.Lreator-1.dia-1 e consequente diminuição do TDH de 240, 96, 48, 32, 24, 16 e 12 horas. O reator acidogênico na 2ª etapa foi operado por 417 dias consecutivos e COV de 120 g.DQO.Lreator1.dia-1, produzindo hidrogênio continuamente, alcançado valores de produção bruta de H2 de 7,60 LH2.dia-1. O reator metanogênico foi operado por 251 dias consecutivos, produzindo metano e alcançado valores de produção bruta de CH4 de 5,90 LCH4.dia-1. A eficiência de remoção de DQO do sistema de reatores foi de aproximadamente 90%, com contribuição aproximadamente de 10% para o reator acidogênico e contribuição aproximadamente de 80% para o reator metanogênico. O reator acidogênico alcançou rendimento de produção de hidrogênio por kg de melaço aplicado de 392 LH2.kgmelaço-1 e o reator metanogênico de 387 LCH4.kgmelaço-1. Para finalidade de comparações e aplicabilidade, o ganho energético global do sistema de reatores de duas fases foi de aproximadamente 5,7 kWh.kgmelaço-1 (1,4 kWh.kgmelaço-1 para o reator acidogênico e 4,3 kWh.kgmelaço-1 para o reator metanogênico). A produção continua de H2 obtida neste estudo está relacionada à associação das vias dos ácidos produtores de hidrogênio já consolidados pela literatura pertinente (acético e butírico) e pela produção de hidrogênio pela rota do ácido lático, devido a associação entre as comunidades de microrganismos estabelecidas no reator. O sequenciamento massivo MiSeq mostrou a seleção de diversos gêneros de microrganismos com redundância funcional e pertencentes principalmente aos Filos Firmicutes, Proteobacteria e Thermotogae, tais como Clostridium sensu stricto, Thermohydrogenium, Thermoanaerobacterium e Cellulosibacter (Firmicutes); Pseudomonas, Enterobacter, Shewanella e Petrobacter (Proteobacteria) e Fervidobacterium (Thermotogae). Microrganismos produtores de ácido lático também foram selecionados tais como: Lactobacillus, Leuconostoc, Sporolactobacillus e Trichococcus. Dos pontos de vista científico e tecnológico este estudo deu mais um passo para a compreensão dos bioprocessos envolvidos nos sistemas anaeróbios em dois estágios produzindo H2 e CH4 continuamente por longo período de tempo. / Anaerobic digestion has shown as an interesting process for renewable energy production (H2 and CH4), for a wide variety of organic compounds (carbon source). This study aimed to advance the understanding of a two-stage process anaerobic system (acidogenic bioreactor followed by methanogenic bioreactor) under thermophilic condition (55°C) fed with molasses, a sugarcane industry by-product. The experiments were conducted at up-flow structured bed reactors and sugarcane molasses was diluted with tap water, to adjust the concentration to the wastewater treatment. At first stage two acidogenic reactors were operated in parallel to evaluate different source of inocula and support bed, to obtain continuous and stable hydrogen production. It was applied 2.5, 5 and 10 gCOD.L-1 resulting in OLR of 30, 60 and 120 g.COD.Lreactor-1.day-1, with HRT fixed at 2 hours of hydrogenase gene was detected in both reactors but with higher number of copies of the gene in the reactor that showed higher hydrogen production: the reactor sed with sludge of UASB reactor and using polyurethane foam as support material. To this reactor was coupled a methanogenic reactor fed with effluent from acidogenic reactor and operated with increasing OLR (1, 2, 5, 7, 14, 17 e 26,5 gCOD.Lreactor-1.day-1) decreasing the HRT (240, 96, 48, 32, 24, 16 and 12 hours). The acidogenic reactor was operated during 471 days with OLR of 120 g.COD.Lreactor-1.day-1, with HRT fixed at 2 hours, with continuous hydrogen production with a gross production of 7.60 LH2.day-1. The methanogenic reactor was operated for 251 days, with continuous methane production of up to 5.90LCH4.day-1. The COD removal efficiency using the two-stage system was approximately 90% , with 10% contribution by the acidogenic reactor and 80% contribution by the methanogenic reactor. The acidogenic reactor achieved hydrogen yield per kg of applied molasses equal to 392 LH2.kgmolases-1. The methanogenic reactor achieved methane yield per kg of applied molasses equal to 387 LCH4.kgmolasses-1. For comparison and applicability purposes, the overall energy yield using the two stage reactor system was approximately 5.7 kWh.kgmolasses-1 (Acidogenic reactor 1.4 kWh.kgmolasses-1 and Methanogenic reactor 4.3 kWh.kgmolasses-1). The continuous production of H2 obtained in this study is related to the association of the hydrogen producer acids pathway established by the relevant literature (acetic and butyric) and the hydrogen production by the lactic acid pathway due to the microorganisms association established in the reactor. Metagenomic analysis by MiSeq Plataform revealed that hydrogen production was due the selection of microorganisms with functional redundancy mainly of Phyla Firmicutes, Proteobacteria and Thermotogae, such as Clostridium sensu stricto, Thermohydrogenium, Thermoanaerobacterium, Cellulosibacter (Firmicutes); Pseudomonas, Enterobacter, Shewanella and Petrobacter (Proteobacteria) and Fervidobacterium (Thermotogae). Genera of acid latic producers, such as Lactobacillus, Leuconostoc, Sporolactobacillus and Trichococcus, were also selected. From the scientific and technological point of view this study has taken another step towards the understanding of bioprocesses involving two stage anaerobic systems for a long term continuous production of H2 and CH4.

A presente pesquisa teve por objetivo avaliar a produção biológica de hidrogênio em longo prazo, e os impactos da separação das principais etapas da digestão anaeróbia, acidogênese e metanogênese, sobre a eficiência do tratamento em reatores de leito fixo estruturado e sobre o desempenho da filtração em biorreatores com membrana. O efluente utilizado foi à base de sacarose e a temperatura foi mantida em 30ºC. Na primeira etapa experimental, avaliou-se a produção de H2 em três configurações de reatores: leito fixo estruturado (FB), UASB granular (UG) e UASB floculento (UF-1). Na segunda etapa experimental, um reator UASB acidogênico (UF-2) foi combinado a um reator metanogênico de leito fixo estruturado (RM). Um reator de estágio único de leito fixo estruturado (RU) foi operado em paralelo. Na última etapa experimental, foram avaliados dois biorreatores anaeróbios conjugados com módulos externos de membranas tubulares, nomeadamente 1-AnMBR, que foi alimentado com efluente bruto, e 2-AnMBR, que foi alimentado com efluente acidificado. Na primeira etapa, sob um TDH de 3,3 h (COV = 33 gDQO.L-1d-1), os reatores FB, UG e UF-1 apresentaram produção de H2 contínua, porém instável, com rendimentos de aprox. 1,5, 0,8 e 1,2 molH2.mol-1sacaroseconsumida, respectivamente. O reator UF-1 apresentou uma estabilidade relativamente melhor e, por isso, esta configuração foi utilizada nos experimentos seguintes. No reator UF-2, aumentou-se o TDH para 4,6 h (COV = 25 gDQO.L-1d-1), o que significativamente promoveu a melhoria do desempenho. Nenhum alcalinizante foi adicionado e o pH do efluente permaneceu em torno de apenas 2,7. Contudo, uma produção de H2 contínua, estável e por longa duração foi atingida, de 175 mLH2.L-1h-1 (= 4,2 LH2.L-1d-1), com rendimento de 3,4 molH2.mol-1sacaroseconsumida, concomitante com a produção de ácido acético e etanol. Nos reatores metanogênicos, o TDH aplicado foi gradativamente reduzido (53-18 h no RM e 56-23 h no RU). Após os sistemas atingirem estabilidade, os valores de DQO permaneceram inferiores no efluente do RM, sobretudo pela redução da concentração de SSV, equivalente a 92 mg.L-1, enquanto que no RU essa concentração foi de 244 mg.L-1. No final da operação, o rendimento energético do sistema de dois estágios foi de 20,69 kJ.g-1DQOadicionada, sendo 90% proveniente do CH4 e 10% do H2. Este rendimento foi 34% maior do que o obtido no reator de estágio único, que foi de 15,48 kJ.g-1DQOadicionada. Por fim, avaliando-se o desempenho da filtração nos biorreatores com membrana, verificou-se que a permeabilidade operacional foi, na maior parte do tempo, superior no 2-AnMBR. A pré-acidificação do efluente levou à redução de cerca de 56-59% na concentração de sólidos voláteis suspensos e totais no 2-AnMBR e à modificação no perfil do tamanho das partículas. No 1-AnMBR, porém, não havia partículas de pequenas dimensões, tais quais encontradas no reator acidogênico, indicando reduzido crescimento suspenso de bactérias acidogênicas. Embora os valores de fluxo crítico tenham sido muito semelhantes para ambos os AnMBR, testes de resistência específica da torta indicaram maior resistência do lodo do 1-AnMBR (1,02 x 1018 m-1), comparado ao lodo do 2-AnMBR (1,03 x 1012 m-1) e ao lodo acidogênico (7,44 x 1011 m-1). Portanto, essa pesquisa demonstrou, por meio da aplicação do tratamento anaeróbio em dois estágios, a viabilidade da produção contínua de hidrogênio em pH extremamente ácido e com mínimos requerimentos operacionais, a redução da concentração de sólidos suspensos no efluente de reatores de leito fixo estruturado, o potencial de aumento da recuperação de bioenergia e de redução da incrustação em membranas de ultrafiltração. / This study assessed long-term hydrogen production and the impacts of separating the main stages of anaerobic digestion (acidogenesis and methanogenesis) on treatment efficiency in structured fixed-bed reactors and on filtration performance in anaerobic membrane bioreactors. Sucrose based wastewater was used and the temperature was maintained at 30°C. In the first experimental phase, H2 production was evaluated in three different acidogenic reactors: structured fixed-bed (FB), granular UASB (UG) and flocculated UASB (UF-1). In the second experimental phase, an acidogenic UASB reactor (UF-2) was combined with a structured fixedbed methanogenic reactor (RM). A single-stage structured fixed-bed reactor (RU) was operated in parallel. In the last experimental phase, two sidestream anaerobic membrane bioreactors were evaluated: 1-AnMBR, which was fed with raw effluent; and, 2-AnMBR, which was fed with biologically acidified effluent. During the first operational phase, under an HRT of 3.3 h (OLR = 33 gCOD.L-1d-1), the FB, UG and UF-1 reactors showed continuous but unstable H2 production, with yields of approximately 1.5, 0.8 and 1.2 molH2.mol-1sucroseconsumed, respectively. The UF-1 reactor showed relatively better stability; therefore, this configuration was used in the next experiments. In the UF-2 reactor, the HRT was increased to 4.6 h (OLR = 25 gCOD.L-1d-1), which significantly improved the overall performance. No alkalizing agent was added, and effluent pH values remained around only 2.7. However, continuous, stable and long-term H2 production was achieved of 175 mLH2.L-1h-1 (= 4.2 LH2.L-1h-1), with yields of 3.4 molH2.mol-1sucroseconsumed, concomitant with acetic acid and ethanol production. In the methanogenic reactors, the HRT was gradually reduced and, when the systems reached stability, COD values remained lower in the RM effluent. This was mainly due to the reduction of VSS concentrations, equivalent to 92 mg.L-1, while in the RU this value was 244 mg.L-1. At the end of the operation, the energy yield of the two-stage system was 20.69 kJ.g-1CODadded with 90% coming from CH4 and 10% from H2. This yield was 34% greater than that obtained in the single-stage system, which was 15.48 kJ.g-1CODadded. Finally, regarding the filtration performance in the membrane bioreactors, the operational permeability was higher in the 2- AnMBR most of the time. The pre-acidification of the effluent led to the 56-59% reduction in the volatile total and suspended solid concentrations, and to modification in the particle size profile in the 2-AnMBR. Nevertheless, in the 1-AnMBR, there were no small particles such as were found in the sludge of the acidogenic reactor, indicating less suspended growth of acidogenic biomass. Although the critical flux values were very similar for both AnMBRs, a higher specific cake resistance was verified in the 1-AnMBR sludge (1.02 x 1018 m-1), as compared to the 2-AnMBR sludge (1.03 x 1012 m-1) and to the acidogenic sludge (7.44 x 1011m-1</sup). Therefore, this study demonstrated, through the application of two-stage anaerobic treatment, the viability of continuous hydrogen production in extreme acid pH and with minimum operating requirements, the reduction of solid concentrations in the effluent of structured fixed bed reactors, as well as the potential for increased bioenergy recovery and for fouling reduction of ultrafiltration membranes.