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Teaching staff who work with children with Autism Spectrum Disorders to evaluate the treatment integrity of Discrete-Trials teaching sessionsWightman, Jade 12 January 2016 (has links)
Treatment integrity is an important component of behavioural interventions, however few studies have examined methods to teach supervisors to evaluate the treatment integrity of such interventions applied by front-line staff. The purpose of the current study was to evaluate the effectiveness of a self-instructional package to teach individuals to evaluate the treatment integrity of discrete-trials teaching (DTT) sessions using the Discrete-Trials Teaching Evaluation Form (DTTEF). Participants consisted of six staff from the St.Amant Autism Programs. In a modified multiple-baseline design across a pair of participants, and replicated across two more pairs, at Baseline, a participant observed a confederate who role-played an instructor teaching three tasks to a confederate who role-played a child with autism spectrum disorder (ASD). Each participant was required to evaluate sessions taught with (a) low integrity, (b) moderate integrity, or (c) high integrity. During training, participants studied a self-instructional package. At Post-training, participants were assessed on the same tasks as during Baseline. During Generalization, participants were assessed evaluating the treatment integrity of three videos of an Autism Tutor administering DTT to a child with ASD. Finally, during a seven-month Follow-up, four participants were available and were assessed evaluating the treatment integrity of a confederate instructor teach a confederate child with low, moderate, and high integrity. Results demonstrated that after an average of 1 hour and 16 minutes of training, there was an immediate increase in accuracy across all participants. Specifically, mean accuracy increased from 47.6% in Baseline to 84.7% at the Post-training assessment (a 37.1% increase). All participants showed excellent generalization results, and three of the four participants who were available at the Follow-up assessment performed at a high level. These results suggest that the training package has potential to be used as an effective method to train staff who work with children with ASD to evaluate the treatment integrity of DTT sessions. / February 2016
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Impact of GNSS singular events on the integrity of airport navigation systems / Impact des évènements singuliers GNSS sur l'intégrité des systèmes de navigation aéroportuairesMontloin, Leslie 10 July 2014 (has links)
Les systèmes GNSS sont actuellement utilisés en aviation civile pour estimer la position et la vitesse de l'avion pendant les phases de route jusqu'aux approches de précision. Etendre l'utilisation de GNSS aux opérations de surface en environnement aéroportuaire et sous de faibles conditions de visibilité reste un challenge pour la communauté aviation civile. En effet, durant ces opérations, les mesures GNSS peuvent être affectées par des évènements singuliers tels que les multi-trajet ou les anomalies ionosphériques. Ces évènements peuvent engendrer des erreurs de position jugées inacceptables en termes de précision et d'intégrité pour assurer le guidage de l'avion. Les algorithmes de surveillance d'intégrité GNSS actuellement utilisés ne sont pas conçus pour prendre totalement en compte les effets de tels évènements. Il est essentiel de développer des algorithmes de surveillance conçus pour protéger les utilisateurs des effets de tels évènements afin de pouvoir utiliser GNSS pour le guidage de l'avion en milieu aéroportuaire et sous de faibles conditions de visibilité. Afin de concevoir de tels algorithmes de surveillance d'intégrité, il est nécessaire de développer des modèles d'erreurs de mesures GNSS et des modèles de pannes GNSS. La thèse a été principalement orientée vers la conception de modèles d'erreurs de mesures GNSS dues aux multi-trajets et vers le développement de modèles de pannes GNSS dues aux multi-trajets. Pour ce faire, un modèle d'erreurs multi-trajets GNSS sur les mesures bi-fréquence GPSL1C+GPSL5 et GalileoE1+GalileoE5a a d'abord été proposé. Ensuite, l'impact des multi-trajets sur l'erreur de position a été étudié. Pour cette étude, un algorithme de couplage serré GPS+Galileo/IRS a été considéré. Cet algorithme est basé sur un filtre de Kalman linéarisé. Une analyse théorique et quantitative a été conduite pour étudier l'impact des erreurs de mesures GNSS dues aux multi-trajets sur le biais et sur la matrice de covariance de l'erreur de position horizontale en sortie de l'algorithme de positionnement considéré. Finalement, un modèle de pannes GNSS dues aux multi-trajets a été proposé. Ce modèle décrit la signature des pannes multi-trajets, les facteurs influençant cette signature, le modèle d'occurrence des pannes multi-trajets ainsi que les conditions d'occurrence de telles pannes / GNSSs are currently used in civil aviation to provide aircraft with position and velocity estimates from en-route to precision approach operations. Extending the use of GNSS to the guidance function during airport surface operations and under zero-visibility conditions remains a challenge. Indeed, during these operations, GNSS measurements may be affected by GNSS singular events, such as multipath or ionosphere anomalies. GNSS singular events may lead to unacceptable position errors in terms of accuracy and integrity for the zero-visibility guidance function. Current GNSS integrity monitoring systems are not designed to totally account for the GNSS singular event effects. The development of GNSS integrity monitoring systems designed to properly protect users from the singular event effects is essential to use GNSS for the guidance function under zero-visibility conditions. GNSS measurement error and integrity failure models are key inputs in the design of GNSS integrity monitoring systems. In this thesis, work has been mainly focused on the development of GNSS multipath measurement errors, on the assessment of the multipath impact on the GNSS-based position error, and on the development of GNSS multipath integrity failure models. For this matter, the dual frequency GPSL1C+GPSL5 and GalileoE1+GalileoE5a multipath pseudo- range error model adapted to airport navigation has been firstly proposed. Next, the impact of multipath on the GNSS-based position error has been assessed. To do so, a double constellation GPS+Galileo/IRS tight coupling algorithm based on a linearized Kalman filter has been selected. The theoretical and quantitative analysis of the impact of the GNSS multipath ranging errors on the horizontal position bias and on the covariance matrix of the horizontal position error have been proposed. Finally, a GNSS multipath integrity failure model has been proposed. The model describes the signature of the GNSS single multipath ranging failures, the factors influencing the signature as well as the occurrence model of these failures and their conditions of occurrence.
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Black-, grey-, and white-box side-channel programming for software integrity checkingLiu, Hong January 1900 (has links)
Doctor of Philosophy / Department of Computing and Information Sciences / Eugene Vasserman / Checking software integrity is a fundamental problem of system security. Many approaches have been proposed trying to enforce that a device runs the original code. Software-based methods such as hypervisors, separation kernels, and control flow integrity checking often rely on processors to provide some form of separation such as operation modes and memory protection. Hardware-based methods such as remote attestation, secure boot, and watchdog coprocessors rely on trusted hardware to execute attestation code such as verifying memory content and examining signatures appearing on buses. However, many embedded systems do not possess such sophisticated capabilities due to prohibitive hardware costs, unacceptably high power consumption, or the inability to update fielded components. Further, security assumption may become invalid as time goes by. For Systems-on-Chip (SoCs), in particular, internal activities cannot be observed directly, while in non-SoCs, sniffing bus traffic between constituent components may suffice for integrity checking.
A promising approach to check software integrity for resource-constrained SoCs is through side-channels. Side-channels have been used mostly for attacks, such as eavesdropping from vibration of glass or plant leaves, fingerprinting machines from traffic patterns, or extracting secret key materials of cryptographic routines using power consumption measurements. In this work, side-channels are used to enhance rather than undercut security. First, we study the relationships between the internal states of a target device and side-channel information. We use the uncovered relationships to monitor the internal state of a running device and determine whether the internal state is an expected one. An unexpected state may be a sign of incorrect execution or malicious activity.
To further explore the possibilities inherent in side-channel-based software integrity checking, we investigate various hardware platforms, representative of different degrees of knowledge of the hardware from the side-channel profiling point of view. In other words, side-channel information is extracted by black-, grey-, and white-box analysis. Each one involves unique challenges requiring different techniques to successfully derive “side-channel profiles”. We can use these profiles to detect unexpected states with extremely high probability, even when an adversary knows that their code may be subject to side-channel analysis, i.e., the methodology is robust to side-channel-aware adversaries.
The research includes: (1) Constructing systematic approaches for black- and grey-box profiling of side channels (and comparing them to white-box analysis); (2) Designing custom measurement instrumentation; and (3) Developing techniques for monitoring and enforcing software integrity utilizing side-channel profiles.
We introduce the term “side-channel programming” to refer to techniques we design in which developers explicitly utilize side-channel characteristics of existing hardware to optimize run-time software integrity checking, creating executable code which is more conducive to side-channel-based monitoring. Compared with other software integrity checking techniques, our approach has numerous benefits. Among them are that the measurement process is non-invasive, non-interruptive, and backward-compatible in that it does not require any hardware modification, meaning our approach works with processors that do not include security features. Our method can even be used to augment existing protection mechanism, as it works even when all security mechanisms internal to the device fail.
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Research on the Relationship between Integrity and Market Economy in ChinaJanuary 2017 (has links)
abstract: The discussion on the question of integrity has a long history. It is regarded as an important research proposition in the fields of Chinese and Western philosophy, law, sociology, ethics, management and economics. Even though the research results are combined with each other for reference, there is still a lack of comprehensive analysis and empirical study on integrity. In the process of transition from planned economy to market economy, a series of problems have arisen. The problem of dishonesty has caused huge losses to the society as a whole. Therefore, it is of great theoretical and practical importance to conduct a quantitative analysis on the topic of trustworthiness, and investigate the relationship between trustworthiness and the economy.
This paper adopts a game theory model to analyze the reasons for corporate dishonesty, and the problem faced by the regulators. Based on the results from the model, we clarify the duties of various subjects (enterprises, governments and credit organizations) in the construction of a society with integrity.
Then, we analyze different cases of dishonesty and point out the channels through which the economy would be affected by the problem of dishonesty. (1) as an institution, integrity helps reduce the transaction cost and prompt market efficiency; (2) integrity serves as a production factor that influences the economy; (3) integrity will affect the economy by influencing the ability of small and medium enterprises to borrow.
Finally, after we establish the relationship between integrity and the market economy, we use survey data to conduct an empirical analysis on the development of integrity in China. The survey data allow us to build a cycle of integrity risk, and identify the current position in the cycle. Besides, we also compare the region difference regarding integrity, which supports the idea that integrity matters for the economic development.
Because the questionnaires are the only way to obtain the data that can be analyzed at present, the paper not only fills in the research gap caused by the lack of data, but also jumps out of the existing research methods, and enriches the empirical work for the study of integrity. / Dissertation/Thesis / Doctoral Dissertation Business Administration 2017
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The blockchain based system to guarantee the data integrity of IIoTShen, Yifei January 2018 (has links)
With the advent of big data era, there is countless data produced from various kinds of machines every second. These data are used to help people to do studies, produce goods, improve the efficiency of the industrial development, and so on. Especially considering the Internet of things which connects all the smart machines together, the importance of data integrity gets unprecedented attention from us. In order to keep the data integrity, blockchain comes to its birth using its own structure to guarantee the data integrity efficiently. This project is focus on the simulation of data system based on the blockchain in the background of industrial internet of things which is shown in a form of a website with coding language jade and the environment is Node.Js. The main task is to measure the time consumed in the process of block mining under different parameters include data length, nonce, difficulty, sensor number in order to find the correlation between block mining time and different parameters. Thus, the rule about the impact of different parameter on the mining time are concluded. According to the rule I found, the evaluation about scalability, efficiency and safety of this system are given and I also summarize two formulas to calculate the efficiency of block mining. Ethical consideration and future work are addressed in the conclusion part.
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Integridade, disponibilidade e acurácia no posicionamento RTK e RTK em rede : Investigação no contexto da rede GNSS ativa do Estado de São PauloBarbosa, Eduardo de Magalhães. January 2010 (has links)
Orientador: João Francisco Galera Monico / Banca: Leonardo Castro de Oliveira / Banca: Mauro Issamu Ishikawa / Resumo: Os avanços tecnológicos nos métodos de posicionamento têm possibilitado o desenvolvimento de metodologias que viabilizam a sua utilização pelo usuário numa diversidade de aplicações. Um dos métodos de posicionamento GNSS (Global Navigation Satellite System) de grande destaque é o RTK (Real Time Kinematic) utilizando o conceito de rede de estações. Esse método utiliza a infra-estrutura de uma rede de estações de referência, para disponibilizar correções ao usuário. O conceito clássico do RTK tem como princípio básico a alta correlação dos erros provocados pela ionosfera, troposfera e órbita dos satélites na estação de referência e em uma estação próxima de interesse. No entanto, com o afastamento entre o usuário e a estação base, a eficiência do método é degradada, pois a correlação dos erros é reduzida. Mas, quando se utiliza uma rede de estações de referência, pode-se realizar a modelagem dos erros na área de abrangência da rede. Esta concepção é denominada de RTK em Rede. No que concerne a rede de estações de referência GNSS, utilizou-se a rede estabelecida no oeste do estado de São Paulo (rede GNSS-SP). O software adotado para realizar o processamento em rede foi o GPSNet na versão 2.73. Outra questão levantada foi com relação aos padrões disponíveis para a transmissão dos dados ao usuário, tais como o RTCM e CMR, mostrando as evoluções e aplicações. A questão do enlace de comunicação (link) também foi abordada, mostrando os principais tipos disponíveis, mas com ênfase na telefonia móvel que foi utilizada para transmitir os dados via internet. Foram conduzidos vários experimentos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Technological advances in positioning methods have enabled the development of methodologies that allow its use in a great variety of applications. One method of GNSS (Global Navigation Satellite System) positioning of high performance is the RTK (Real Time Kinematic) network. This method uses the infrastructure of a network of reference stations. The original concept of RTK is the high correlation of the errors caused by ionosphere, troposphere and satellite orbit at a reference station and at a nearby station of interest. However, increasing the distance between the user and the base station, the efficiency of the method is degraded, because the error correlation is reduced. But when using a network of reference stations, a better error modeling in the area of the network is possible. This concept is the so called Network RTK. Regarding the network of GNSS reference stations, in this work the established network in the western state of São Paulo (GNSS network-SP) was used. The software adopted to perform the tests in the network was that GPSNet, version 2.73. Another issue raised during the investigation was related to the available standards for transmitting data to the user, such as CMR and RTCM. The developments and applications were presented. The issue of communication link (link) has been addressed, showing the main available types, but with emphasis on mobile phone that was the technology used to transmit data via the Internet. Several experiments were conducted in different locations to examine the integrity, availability and accuracy for RTK positioning and RTK network. The experiments performed with RTK and RTK network (using the concept of VRS (Virtual Reference Station)) showed some problems, probably related to the density of the network. Among them the accuracy, the initialization time span more than expected or even... (Complete abstract click electronic access below) / Mestre
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Evaluation of Cryptographic PackagesRaheem, Muhammad January 2009 (has links)
The widespread use of computer technology for information handling resulted in the need for higher data protection.The usage of high profile cryptographic protocols and algorithms do not always necessarily guarantee high security. They are needed to be used according to the needs of the organization depending upon certain characteristics and available resources.The communication system in a cryptographic environment may become vulnerable to attacks if the cryptographic packages don’t meet their intended goals. This master’s thesis is targeted towards the goal of evaluating contemporary cryptographic algorithms and protocols collectively named as cryptographic packages as per security needs of the organization with the available resources. The results have shown that there certainly is a need for careful evaluations of cryptographic packages given with available resources otherwise it could turn into creating more severe problems such as network bottlenecks, information and identity loss, non trustable environment and computational infeasibilities resulting in huge response times. In contrast, choosing the right package with right security parameters can lead to a secure and best performance communication environment.
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“Man är lite annorlunda på sociala medier, det blir lite mer filtrerat men det är fortfarande jag” : En fokusgruppanalys av ungas mediepraktiker i relation till identitet ochintegritetKäll Berg, Ellinora January 2018 (has links)
The aim of this essay is to study how youth utilize social media based on themes such as identity and integrity. The focus has been on young people’s use of Instagram. The study is based on qualitative focus groups to collect empiricism. Interviews have been done with four different groups. Two groups of mixed girls and boys and two groups where one was with girls only and one with only boys. The purpose has been to see if there are similarities and / or differences between how girls and boys look at their use of Instagram. The study also concerns the participants' urge to use social networks based on concepts such as confirmation and motivation. The result of this study shows both differences and similarities between the different focus groups. Social media have a function in how young people create their identity and how the identity is maintained. The study also presents that young people have different types of identities depending on context. A similarity between users is the strategic use they have of Instagram.
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Seguimento de recÃm-nascidos egressos de unidade de terapia intensiva neonatal na perspectiva da integralidade da atenÃÃo à saÃde / Follow-up of infants discharged from neonatal intensive care unit from the perspective of the entire health careAlcilÃa Leite de Carvalho 01 August 2013 (has links)
No Brasil, o MinistÃrio da SaÃde, por meio da Secretaria de AtenÃÃo à SaÃde, preconiza o acompanhamento dos recÃm-nascidos de risco e de alto risco pelos ambulatÃrios de seguimento especÃficos, bem como pela atenÃÃo bÃsica. Determina como de indiscutÃvel relevÃncia a interlocuÃÃo entre os serviÃos e, ainda, na linha de cuidado perinatal, fundamenta os princÃpios assistenciais, na qual estÃo incluÃdas qualidade e integralidade. à vista disso, a pesquisa objetiva compreender aspectos da integralidade da atenÃÃo disponibilizada na rede de serviÃos de Fortaleza aos RN egressos de unidade de terapia intensiva neonatal, com suporte na percepÃÃo dos profissionais de saÃde dos ambulatÃrios de seguimento neonatal. Trata-se de um estudo de caso de natureza qualitativa, no qual foram sujeitos da pesquisa seis mÃdicas, que trabalham em quatro ambulatÃrios de seguimento de Fortaleza. Foram realizadas entrevistas individuais semiestruturadas e o material foi analisado conforme a tÃcnica de anÃlise de conteÃdo. Os resultados foram apresentados em trÃs categorias temÃticas centrais: a) Fatores facilitadores da compreensÃo materna sobre o motivo do seguimento; b) O cotidiano de trabalho nos ambulatÃrios; e c) PercepÃÃo dos profissionais sobre o seguimento ofertado. Constata-se que a compreensÃo materna acerca dos motivos do seguimento guarda relaÃÃo com as informaÃÃes repassadas aos familiares durante o internamento e com a abordagem nas consultas de seguimento. O vÃnculo estabelecido entre o profissional e a famÃlia foi mencionado, tanto como facilitador do seguimento, como da compreensÃo materna sobre ele; e, tambÃm, foi definido como imprescindÃvel, visto que os obstÃculos estruturais ao acompanhamento de casos de maior complexidade sÃo superados com iniciativas individuais dos profissionais, em que a responsabilizaÃÃo à o determinante para assegurar a conduÃÃo, revelando assim as fragilidades estruturais nos serviÃos pesquisados. Existem dificuldades com uma agenda aberta, propiciadora de livre acesso ao ambulatÃrio por parte das mÃes. Ao mesmo tempo, à relatada a frequente procura materna aos serviÃos fora da agenda programada. Por sua vez, a disponibilidade de uma equipe de seguimento compondo a estrutura do ambulatÃrio foi apontada como facilitadora da atenÃÃo. O compartilhamento do seguimento com a EstratÃgia SaÃde da FamÃlia à reconhecido como necessÃrio, nÃo existindo, no entanto, mecanismos institucionais de comunicaÃÃo entre esses dois nÃveis de atenÃÃo. Logo, tal ausÃncia, dentre outros aspectos, redunda na
constituiÃÃo das mÃes e/ou dos familiares em interlocutores principais deste processo. Na pesquisa, evidenciou-se ausÃncia de confianÃa na continuidade do cuidado fora da estrutura do ambulatÃrio. As profissionais entrevistadas percebem uma melhoria na qualidade do atendimento ofertado ao longo dos Ãltimos anos. / In Brazil, the Ministry of Health, through the Secretariat of Attention to Health Care advocates the accompaniment of new born babies at risk and at high risk through the outpatient follow up clinics, as well as through basic care programs. The dialogue between these services and prenatal care is undisputedly relevant, when founded on the principles of care, those being quality and integrality. In light of this, the study aims at understanding aspects of integrality of the care given to newborns leaving neonatal intensive care units of the health care service network in Fortaleza. The study is supported by the perception of the health care staff members in the neonatal follow-up clinics. It is a qualitative case study in which six doctors were the subjects. These doctors work in four follow-up clinics in Fortaleza. Semi-structured individual interviews were used and the information collected was analyzed according to the content analysis technique. The results have been presented in three main categories: a) Factors facilitating the motherâs understanding concerning the reasons for follow up; b) the daily work of the clinics and c) the perception of the doctors regarding the follow-up care which is offered. It was found that the motherâs understanding of the motive for follow-up visits is related to the information given to the family during hospitalization and to the approach taken during the follow-up appointments. The tie established between the doctor and the family was mentioned as a facilitator to follow-up as well as the motherâs understanding of it. It was also found that the doctorsâ individual initiatives were essential in overcoming the structural obstacles for follow-up visits in cases of greater complexity. In these cases accountability is a determining factor to ensure transportation to the clinic. This reveals the fragility of the service structures under study. There are problems with an open schedule which would allow mothers to have free access to the clinic. At the same time, mothers requesting services at unscheduled times was frequently mentioned. Thus, the availability of a follow-up team as part of the clinicâs structure was pointed out as a facilitator for care giving. Sharing follow-up activities with the Strategic Family Health program is recognized as necessary, however the institutional mechanisms of communication between the two levels of health care are non-existent. Thus this lack, as well as other aspects, results in the mothers and/or relatives being the main interlocutors of this process. In the study, the lack of confidence in the continuity of the care outside the clinicâs structure was evident. The
doctors who were interviewed have perceived an improvement in the quality of care offered during recent years.
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ProteÃnas espermÃticas e dinÃmica da cromatina em ruminantes: relaÃÃo com a fertilidade em touros e com o uso de castanha de caju na dieta de ovinos / Sperm proteins and chromatin dynamics in ruminants : relationship with fertility in bulls and with the use of cashew nuts in the diet of sheepRodrigo Vasconcelos de Oliveira 05 July 2013 (has links)
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / The ruminant fertility is influenced by intrinsic sperm factors, like chromatin or proteins. Considering that the reproductive efficiency is dependent on a balanced and feasible nutrition, the cashew nut meal (CNM) is a low cost byproduct that must be analyzed for possible effects on sperm chromatin and proteins.Study 1:. The objectives of study 1 were to determine failures of chromatin condensation, expression levels and cellular localizations of histones; H3.3, H2B and H4, respectively in spermatozoa from low (LF) vs. high fertility (HF) bulls. The data were analyzed by t test and Pearson correlation (P < 0.05). We demonstrated that aniline blue staining was different within LF (1.73 (0.55, 0.19)) and HF Groups (0.67 (0.17, 0.06) (P < 0.0001), which was also negatively correlated with in vivo bull fertility (r = -0.90; P < 0.0001). Although those histones were consistently immune-detectable and specifically localized in bull sperm, this was not different between the two groups. Except H2B variants, H3.3 and H4 showed 100% identity and conserved among bovine, mouse and human. The H2B variants were more conserved between bovine and human than those of mouse. In conclusion, we showed that H2B, H3.3 and H4 were detectable in bull spermatozoa and that sperm chromatin condensation status, changed by histone retention, is related with bull fertility. Study 2: The objectives of study 2 were evaluate the effects of 13% of CNM inclusion in the diet of Morada Nova rams on the semen parameters, chromatin integrity and sperm proteins. Twenty rams were distributed in two equal groups: cashew nut group (CNG) and control group (COG) that received 13% and 0% of CNM in the diet for 90 days, respectively. The groups were compared for live weight, scrotal circumference, seminal parameters, chromatin integrity and sperm protein profile at 0, 45 and 90 days of the experiment. The data were evaluated by GLM for repeated measures (P < 0.05). At 90 days, CNG (69.00% (7.38; 2.33)) presented percentage of motile sperm superior than control group (60,00% (9,43; 2,98)) (P<0.05). There was not effect from the diet with CNM on chromatin integrity. But, the percentages of protein expression from ODF1 and H2B were larger in the CNG (P<0.05). The proteins: ODF1, GPX4, FTL and H2B were negatively correlated with sperm chromatin quality. In conclusion, the cashew nut meal did not affect negatively the semen quality. / A fertilidade em ruminantes à influenciada por fatores intrÃnsecos espermÃticos como a cromatina e as proteÃnas. Considerando que a eficiÃncia reprodutiva do macho depende de uma nutriÃÃo balanceada e viÃvel, o farelo de castanha de caju (FCC) à um subproduto de baixo custo que deve ser analisado quanto a possÃveis efeitos na integridade cromatÃnica e proteica dos espermatozoides. Estudo 1: O estudo 1 teve como objetivos determinar: falhas na condensaÃÃo da cromatina, nÃveis de expressÃo e localizaÃÃo celular das histonas: H3.3, H2b e H4B, respectivamente, em espermatozoides de touros de baixa (BF) e alta fertilidade (AF). Os dados foram avaliados pelo teste t e correlaÃÃo de Pearson (P < 0,05). Os resultados do teste do azul de anilina foram diferentes entre os grupos BF (1.73 (0.55, 0.19)) e AF (0,67 (0,17, 0,06) (P < 0,0001), os quais tambÃm foram negativamente correlacionados com a fertilidade in vivo de touros (r = -0,90; P < 0,0001). Apesar das histonas terem sido consistentemente imunodetectadas e localizadas nos espermatozoides, estas nÃo apresentaram diferenÃas entre os grupos. As proteÃnas H3.3 e H4 apresentaram 100% de identidade e foram conservadas entre bovinos, murinos e seres humanos. Entretanto, as variantes H2B foram mais conservadas entre touros e humanos do que entre humanos e camundongos. Em conclusÃo, as proteÃnas H2B, H3.3 e H4 foram detectÃveis em espermatozoides de touros e a condensaÃÃo da cromatina espermÃtica, alterada pela retenÃÃo de histonas, à relacionada com a fertilidade de touros. Estudo 2: O estudo 2 objetivou avaliar os efeitos da inclusÃo de 13% de FCC na dieta de carneiros Morada Nova sobre as caracterÃsticas seminais, integridade de cromatina e perfil das proteÃnas espermÃticas. Vinte carneiros foram divididos em dois grupos: castanha (GCA) e controle (GCO).que receberam na dieta 13% e 0% de FCC durante 90 dias, respectivamente. Os grupos foram comparados quanto ao peso vivo, circunferÃncia escrotal, parÃmetros seminais, integridade de cromatina e perfil das proteÃnas espermÃticas aos 0, 45 e 90 dias de experimento. Os dados foram analisados pelo mÃtodo GLM para medidas repetidas (P < 0,05). Aos 90 dias o GCA (69,00% (7,38; 2,33) apresentou porcentagem de espermatozoides mÃveis superior ao GCO (60,00% (9,43; 2,98)) (P<0.05). NÃo houve efeito da dieta contendo FCC sobre a integridade da cromatina. PorÃm, os percentuais das proteÃnas ODF1 e H2B foram mais elevados nos carneiros do GCA (P < 0,05). As proteÃnas: ODF1, GPX4, FTL e H2B foram negativamente correlacionadas com a qualidade da cromatina espermÃtica. Em conclusÃo, a inclusÃo de FCC na dieta de carneiros nÃo afetou negativamente a qualidade seminal.
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