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Preparação e caracterização de estruturas polimórficas da tolbutamida e nifedipina / Preparation and characterization of polymorphic structures of the tolbutamide and nifedipineKellen Christina Dutra de Souza 29 July 2005 (has links)
Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro / Neste estudo foram preparados polimorfos do fármaco tolbutamida, um hipoglicemiante oral usado no tratamento dos Diabetes Mellitus tipo II. Foram também preparados polimorfos da nifedipina, fármaco usado no tratamento das desordens cardiovasculares, como angina pectoris e hipertensão. A preparação dos polimorfos foi mediada por solvente, ou seja, foi em função do solvente usado nas etapas de cristalização e de precipitação das espécies. Um método de resfriamento rápido por nitrogênio líquido também foi utilizado. Técnicas analíticas como a espectrofotometria de infravermelho, a calorimetria diferencial de varredura, a difratometria de raio-X e a microscopia eletrônica de varredura foram úteis para a caracterização dos produtos obtidos experimentalmente. Os resultados comprovaram que dois polimorfos da tolbutamida foram preparados, ambos com estrutura cristalina. No caso da nifedipina, dois polimorfos foram preparados e a caracterização mostrou que um destes foi obtido num estado amorfo enquanto o outro estava sob forma cristalina. A instabilidade da nifedipina no estado amorfo foi monitorada pela técnica de calorimetria diferencial de varredura que, através de diferentes curvas, mostrou uma transformação rápida para uma estrutura cristalina. Esta mesma técnica aliada à termogravimetria confirmou a obtenção de um terceiro produto da nifedipina, de estrutura cristalina, que foi considerado um pseudopolimorfo por ser uma espécie solvatada. Ao final do procedimento experimental e da avaliação dos resultados foi sugerido um esquema, passo a passo, para obtenção e caracterização de polimorfos de uma substância / In this study the polymorphs of tolbutamide, an oral hypoglicemiant used on Diabetes Mellitus type II treatment, and of nifedipine, a drug used in the cardiovascular disorders treatment, were prepared. All crystalline forms were obtained by crystallization from different solvents. Tolbutamide was isolated only in crystalline forms and nifedipine in two crystalline forms and in the amorphous form prepared by melting and subsequent cooling. The polymorphs from each drug were characterized by powder x-ray diffraction (PDRX), infrared spectroscopy (IR), Raman spectroscopy (FT-RAMAN), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). The results proved that two different crystalline forms of tolbutamide were obtained and two crystalline form to nifedipine, one of them as a pseudo-polymorph. The characterization confirmed that melting and quickly cooling procedure prepared amorphous nifedipine. Differential scanning calorimetry technique generated curves whose data proved that the amorphous nifedipine is a very unstable form. Thermogravimetry confirmed a pseudo-polymorphs preparation of nifedipine. In spite of the modification observed on the profile of X-ray diffraction, because of the solvent present, was possible to prove that this solvated form have an crystalline structure. A methodology was proposed step by step to prepare and characterize polymorphs of a substance
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A instabilidade genômica como fator prognóstico e diagnóstico na progressão de queratose actínica para carcinoma espinocelular humano / Genomic instability as a prognostic and diagnostic factor on the progression of human actinic keratosis, to squamous cell carcinomaLuciana Sanches Cabral 19 June 2007 (has links)
A instabilidade genômica tem sido amplamente usada para caracterizar células cancerosas. Alterações genéticas em queratose actínica (QA) e carcinoma espinocelular (CEC) foram investigadas pelo método de random amplified polymorphic DNA (RAPD) e análise de microssatélites com o objetivo de encontrar marcadores moleculares para auxiliar o prognóstico e o diagnóstico médico. O DNA foi obtido de pacientes brasileiros cirurgiados e tratados no Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, totalizando oito QAs, 24 CECs, e tecidos normais e/ou leucócitos correspondentes. Os microssatélites estudados foram D6S251, D6S252, D9S15, D9S50, D9S52, D9S180, D9S196, D9S280 e D9S287, tendo em vista a detecção de instabilidade genômica representada por perda de heterozigosidade (LOH) e instabilidade de microssatélites (MSI). Os \"primers\" usados para comparar os padrões de RAPD foram OPA-2, OPA-7, OPA-13, OPA-17, OPB-8, OPB-13, OPB-17 e OPB-19. Foi obtida correlação significativa na progressão de QA (1/8) para CEC (5/22) referente ao microssatélite D6S251. As diferenças nos padrões de DNA obtidos pelo método RAPD comparados aos controles foram maiores em lesões com maior grau de severidade segundo critério histológico. O mesmo padrão RAPD foi observado no controle e no tumor em 27% QA, 24% CEC I, 9% CEC II e 0% CEC III. Estes resultados mostram que o microssatélite D6S251 e o método de RAPD são informativos, podendo ser potenciais candidatos para auxílio no diagnóstico e prognóstico de QA e CEC. / Genomic instability has been widely used to characterize cancer cells. Genetic alterations in human actinic keratosis (AK) and squamous cell carcinomas (SCC) were investigated by the random amplified polymorphic DNA (RAPD) method, and microsatellite analysis. DNA was obtained from Brazilian patients diagnosed and treated in the School of Medicine of University of Sao Paulo out Clinics Hospital. Eight AKs, 24 SCCs, and 4 BCCs, matched to normal skin tissue and/or leukocytes were studied. Microsatellite patterns were obtained with primers specific to amplify D6S251, D6S252, D9S15, D9S50, D9S52, D9S180, D9S196, D9S280, and D9S287, in search of detection Loss of heterozygosity (LOH) and Microsatellite instability (MSI). The RAPD primers were: OPA-2, OPA-7, OPA-13, OPA-17, OPB-8, OPB-13, OPB-17, and OPB-19. A significant correlation was obtained regarding the progress of AK (1/8) to SCC (5/22) detected with the D6S251 microsatellite. DNA fingerprint obtained with RAPD primers were altered in increasing number of samples, according to their histological degree of differentiation. Similar RAPD patterns were observed in tumor and control in 27% AK, 24% SCC I, 9% SCC II, and zero SCC III. These results suggest microsatellite D6S251 and RAPD method to be potential tools in diagnosis and prognosis of AK and SCC.
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Design and Implementation of a Graceful Degradation Approach for Polymorphic Role Invocation in Object TeamsKummer, Cornelius 07 September 2021 (has links)
In the ever-evolving world of modern software engineering, dynamic and context-dependent adaptability becomes increasingly important. A promising new paradigm that has been proposed is role-oriented programming, an extension of object-oriented programming which allows collaborative relationships of objects to be modeled. Through the introduction of roles and contexts, the behavior of objects can be adapted at run-time via addition or modification of attributes and methods. This dynamism however incurs a high overhead, especially in the area of role function invocation. Recent research has found a remedy inspired by polymorphic inline caches, allowing reuse of so-called dispatch plans which encode the steps directly required for the execution of adaptations. With this optimization, an average speedup of 4.0× was achieved in static contexts and 1.1× in variable contexts. Still, performance sharply drops off at a certain degree of volatility as a consequence of cache capacity exhaustion. This thesis presents a fallback mechanism that is to be used at highly variable call sites which would normally cause a significant slowdown with the new approach. In addition, an optimized reuse mechanism is proposed, further improving execution efficiency. Evaluation through benchmarking shows complete elimination of the aforementioned overhead, meaning a speedup of 16.5×, while the previously achieved speedup is maintained.
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Crystal Engineering in Nanoporous MatricesGraubner, Gitte 12 February 2015 (has links)
As former studies reveal, the nanoporous confinement could have influence on polymorphic drug crystallization. However, little attention has been paid to the question how crystallization of the commonly polymorphic drugs in nanoporous matrices influences the drug release. As a consequence, sufficient information about the crystallization conditions and their influence on phase behavior, crystal texture, and stability of polymorphs should be retrieved prior to drug delivery experiments. Drug release should be polymorph-selective and even crystal face-specific. Therefore, the topic of this PhD thesis is the systematic investigation of crystallization parameters (e.g., pore morphology, thermal history, presence or absence of a bulk surface reservoir) and their influence on the nucleation and crystal growth of the two selected model compounds in nanoporous matrices: acetaminophen (ACE) and n-tetracosane. Both are confined to two host-systems: AAO containing aligned cylindrical, isolated pores and CPG containing curved, interconnected pores. The guest materials inside the two model matrices have been investigated with X-ray diffraction (WAXS) and differential scanning calorimetry. In the first part it is shown that the nanopore morphology of the host systems determines into which polymorphic form ACE crystallizes. Moreover, the pore morphology influences the kinetics of solid/solid transitions. In AAO uniformly oriented form III crystals are converted into also uniformly oriented form II crystals by a solid/solid transition. Such a phase transition is kinetically suppressed in CPG membranes due to the curved pore morphology. In the second step, polymorph-specific release experiments with ACE from AAO membranes reveal that the drug dissolution is not exclusively diffusion-limited and can be described by the Korsmeyer-Peppas model. Dissolution of crystalline ACE having rough crystal faces exposed to the environment is nearly as fast as release of amorphous ACE. Encapsulating of ACE in AAO nanopores with a PLLA polymer retard the drug dissolution but does not modify the release kinetics. In the third part of this thesis crystallization of n-tetracosane, a saturated hydrocarbon, in nanoporous matrices was studied. n-Tetracosane shows inside AAO membranes the rotator phase sequence: triclinic−RV−RI−RII−liquid. Further, the long axes of the n-tetracosane molecules are oriented normal to the AAO pore axes. In general, n-tetracosane under confinement shows a more complex phase behavior than the polymeric analogue polyethylene. The presented work expands the available strategies for mesoscopic crystal engineering. The methods might be transferred into other areas of interest such as polymorphism screening or preparation of different types of nanowires with customized optoelectronic or ferroelectric properties.
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ChaperonLondon Ante Paris, Patricia 17 November 2023 (has links)
Eine polymorphe Orbitalskulptur, die aus einer 3D-Kohlenstoff -Betonstruktur besteht, die von einem Polypeptidnetzwerk inspiriert ist, das ein geringeres Gewicht und eine geringere Zähigkeit bewirkt und auf einer überfluteten Stadtstraße steht, die von der anthropozentrischen Wärmeakkzeleration herrührt. - Meine künstlerische Praxis besteht aus partizipativen Performances, Malerei und Skulptur im Zusammenspiel von Tanz, Musik, wacher Zeitgenossenschaft und farbintensiven Werken, denn alles Sichtbare ist nur durch die Farbe begehrenswert.
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PROCESS INTENSIFICATION TECHNIQUES FOR COMBINED COOLING & ANTISOLVENT CRYSTALLIZATION OF DRUG SUBSTANCESShivani A Kshirsagar (11000124) 14 October 2022 (has links)
<p>Crystallization is a key solid-liquid separation and purification technique used in pharmaceutical industry. Some of the critical quality attributes (CQAs) of a product from crystallization process include crystal size distribution (CSD), purity, polymorphic form, morphology, etc. Different size and polymorphs of a drug substance may have different dissolution profiles and different bioavailability, which can have adverse effect on human health. Therefore, it is important to design and control crystallization process to meet product CQAs. In recent years, drug substances are becoming more complex, often being heat sensitive, which may limit the temperature that can be used in the crystallization step. Consequently, the traditional cooling only crystallization may not be well suited to recover the high value drug substances. For these systems, antisolvent crystallization is typically employed to improve the yield. On the other hand, the solvent composition can significantly impact the polymorphic outcome. Therefore, designing combined cooling and antisolvent crystallization (CCAC) processes to solve the challenges of active pharmaceutical ingredient (API) crystallization in a highly regulated environment is a complex engineering problem. </p>
<p>With rising energy costs and intense price competition from generic pharmaceutical companies, the pharmaceutical industry is looking for ways to reduce the cost of manufacturing via process intensification (PI). This thesis focuses on different PI techniques for CCAC of drug substances. Continuous or smart manufacturing is gaining popularity due to its potential to lower the cost of manufacturing while maintaining consistent quality. Continuous crystallization is an important link in the continuous manufacturing process. The first part of the thesis shows PI of a commercial drug substance, Atorvastatin calcium (ASC) for target polymorph development via continuous CCAC using an oscillatory baffled crystallizer (OBC). An existing batch CCAC process for ASC was compared with the continuous CCAC in OBC and it was found the continuous process 30-fold more productive compared to the batch process. An array of process analytical technology (PAT) tools was used in this work to assess key process parameters that affect the polymorphic outcome and CSD. The desired narrower CSD product was obtained in the OBC compared to that from a batch crystallizer.</p>
<p>The next part of the thesis focused on model-based PI technique for efficient determination of crystallization kinetics of a polymorphic system in CCAC. A novel experimental design was proposed which significantly reduced the number of experiments required to determine crystallization kinetics in a CCAC process. The kinetic parameters were validated, and a validated polymorphic model was used to perform an in-silico design of experiment (DoE) to develop a design space that can be used to identify operating conditions to achieve a desired crystal size and polymorphic form. </p>
<p>The final part of the thesis combines the experimental and model-based approach for designing a continuous CCAC process for ASC in a cascade of Coflore agitated cell reactor (ACR) and three-stage mixed suspension mixed product removal (MSMPR). A combined artificial neural network (ANN) and principal component analysis (PCA) method was used to calibrate an ultraviolet (UV) probe which was used to monitor ASC solute concentration in the cascade process. The crystallization kinetic parameters were estimated in ACR and MSMPR which was used to build a digital model of the cascade process. The digital model was then used to obtain a design space with different temperature profile in the three-stage MSMPR that yielded narrow CSD of ASC form I. Overall, this thesis demonstrates the benefits of applying PI in the CCAC of drug substances using a holistic approach including novel equipment, application of an array of PAT tools, and model-based digital design to achieve desired CQAs of the product.</p>
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Sequentielle Genotypisierung von Pseudomonas aeruginosa-Isolaten und Übereinstimmung von bakteriologischen Proben aus dem oberen und unteren Respirationstrakt von Patienten mit cystischer FibroseJung, Andreas 26 October 2005 (has links)
Die Frage nach adäquaten mikrobiologischen und molekulargenetischen Methoden, um die Kolonisation des Respirationstrakts von Mukoviszidose-Patienten mit Pseudomonas aeruginosa nachzuweisen und zu charakterisieren, wird kontrovers diskutiert. Von 38 klinisch stabilen Patienten mit cystischer Fibrose (CF) wurden sequentiell im Abstand von 18 Monaten Proben aus Rachenabstrich, Sputum und Bronchiallavage (BAL) entnommen und bezüglich Pseudomonas-Nachweis untersucht. Die Pseudomonas-Stämme wurden mittels Random Amplified Polymorphic DNA (RAPD)-Analyse und Pulsfeld-Gelelektrophorese (PFGE) von DNA-Makrorestriktionsfragmenten typisiert und bezüglich der Frage nach genetisch divergierenden Isolaten innerhalb des selben Individuums sowie nach möglichen longitudinalen genetischen Veränderungen evaluiert. Sensitivität, negative und positive prädiktive Werte und Spezifität, um eine P. aeruginosa-Besiedlung zu erkennen, waren 36%, 74%, 83% und 96% im Falle der Kulturen aus dem Oropharynx von nicht-expektorierenden Patienten und 92%, 94%, 100% und 100% für Sputumkulturen von expektorierenden Probanden. RAPD-Analyse und PFGE waren in der Lage, zwischen unterschiedlichen Pseudomonas-Stämmen zu diskriminieren, wobei nur die DNA-Makrorestriktion zwischen Subtypen unterscheiden konnte. Die Genotypen der Pseudomonas-Isolate aus Rachenabstrich und Sputum divergierten in 55% und 40% zu den Isolaten der BAL. Longitudinale Variationen des Genotyps wurden in 62% der Fälle beobachtet, die Hälfte davon war nur mittels bronchoskopisch gewonnener Proben erkennbar. Zusammengefasst besitzen Sputumproben bezüglich des Pseudomonas-Nachweises dieselbe Wertigkeit wie Kulturen aus der BAL, während Rachenabstriche in einer frühen Krankheitsphase für die Charakterisierung der bakteriellen Flora des unteren Respirationstrakts wenig geeignet sind. Die Methode der DNA-Makrorestriktion kann als zuverlässige Technik für epidemiologische Untersuchungen empfohlen werden. Unterschiedliche Genotypen innerhalb desselben Individuums und longitudinale genetische Alterationen sind häufig, jedoch unter Umständen nur bronchoskopisch nachweisbar. / There is controversy about adequate specimen to detect and characterise colonisation of cystic fibrosis (CF) airways by Pseudomonas aeruginosa. Oropharyngeal, sputum and bronchoalveolar lavage (BAL) samples were evaluated sequentially from 38 stable CF patients for the detection of P. aeruginosa. Pseudomonas strains were typed by random amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE) of DNA macrorestriction fragments. The occurrence of genetically different isolates within the same host and longitudinal variations in the genotype during repeated examinations was assessed. Sensitivity, negative and positive predictive values and specificity to detect P. aeruginosa were 36%, 74%, 83% and 96% for oropharyngeal cultures in non-expectorating patients and 92%, 94%, 100% and 100% for sputum cultures from expectorating patients, respectively. RAPD analysis and PFGE were suitable to characterize P. aeruginosa CF isolates, although only DNA macrorestriction was able to distinguish between identical and closely related strains. Genotypes of Pseudomonas isolates recovered from oropharyngeal swabs and sputum differed to the strains recovered by bronchoscopy in 55% and 40%, respectively. In 62% longitudinal variations in the genotype occurred. Half of these alterations were only detectable from bronchoscopically obtained samples. In conclusion, sputum samples have the same value as specimens from BAL to detect P. aeruginosa colonisation, whereas cultures from the oropharynx are not suitable for characterising the bacterial conditions in the CF lungs in an early disease state. DNA macrorestriction is recommended as an excellent tool for epidemiological investigations. Different genotypes within the same host and longitudinal genetic alterations are common and may be detectable in the BAL fluid exclusively.
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Le Législateur incertain. Recherches sur la contribution ascétique de Basile de Césarée / The Uncertain Legislator Research on Basil of Caesarea’s Ascetic ContributionPerrot, Arnaud 12 December 2016 (has links)
Dans la floraison d’écrits monastiques produits au IVe siècle, les Ascetica attribués à Basile de Césarée ont tenu une place particulière, qui est bien connue. Pourtant, la formation du corpus asceticum, le processus d’éditions multiples dont il a été l’objet, mais aussi les options doctrinales qu’il diffuse n’ont pas été éclaircis de façon satisfaisante. Sur la base d’éléments textuels jusqu’ici négligés, le présent travail se propose de réécrire l’histoire du texte et d’interroger la paternité basilienne de la collection. Il existe, en effet, depuis le Ve siècle au moins et jusqu’à l’époque moderne, un courant minoritaire qui doute de l’opportunité d’attribuer le « livre ascétique » au grand Basile et préfère lui donner pour auteur un autre évêque du IVe siècle, Eustathe de Sébaste, moins estimé de la tradition ecclésiastique. Si le doute sur la paternité basilienne des Ascetica est permis, alors il convient de réévaluer la place de Basile de Césarée dans l’histoire littéraire du mouvement monastique. C’est ce que nous nous proposons de faire dans cet ouvrage, en relisant de près des pièces littéraires qui, jusqu’à présent, n’étaient présentées que comme des « annexes » au dossier des Ascetica. / The Ascetica transmitted under the name of Basil of Caesarea have a special importance among the numerous monastic writings which emerged in the 4th century. If this point is very well-known, the gathering of the corpus asceticum, the multiple antique editions of the collection, and the doctrinal options which are found in this sum have not been really enlightened. On the basis of neglected textual and paleographic evidence, the present work aims at rewriting the history of the text and questioning the basilian paternity of the collection. From the 5th century to the Modern Times, some readers refused the attribution of the Ascetica to Basil the Great and preferred to ascribe them to another (but problematic) bishop of the 4th century: Eustathius of Sebasteia. If there are good reasons to reject the basilian paternity of the collection, it is necessary to reevaluate the role played by Basil of Caesarea in the literary history of the monastic movement. In the present volume, I try to re-read some literary texts which, until now, were just regarded as appendices to the dossier of the Ascetica.
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Molecular authentication of endangered reptiles for Chinese medicinal materials.January 2001 (has links)
Wong Ka Lok. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 121-129). / Abstracts in English and Chinese. / Acknowledgments --- p.i / Abstract --- p.ii / Table A --- p.v / Table B --- p.vi / Table of Contents --- p.vii / Abbreviations --- p.xi / Chapter Chapter 1 --- Molecular authentication of endangered crocodiles and snakes / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Traditional method of snake and crocodile identification / Chapter 1.2.1 --- Morphology --- p.7 / Chapter 1.2.2 --- Chemical Analysis --- p.9 / Chapter 1.3 --- Molecular Technology in Authentication / Chapter 1.3.1 --- Polymerase Chain Reactions (PCRs) --- p.11 / Chapter 1.3.2 --- Random-primed amplification reaction --- p.12 / Chapter 1.3.3 --- Sequence Characterized Amplified Region (SCAR) --- p.13 / Chapter 1.3.4 --- PCR-RFLP --- p.13 / Chapter 1.3.5 --- DNA sequencing --- p.14 / Chapter 1.4 --- Objectives and strategies of the study --- p.15 / Chapter Chapter 2 --- Materials and General Methods / Chapter 2.1 --- Reagents and Buffers / Chapter 2.1.1 --- Buffers for Total DNA Extraction --- p.17 / Chapter 2.1.2 --- Reagents for Agarose Gel Electrophoresis --- p.17 / Chapter 2.1.3 --- Reagents for Plasmid DNA Preparation --- p.18 / Chapter 2.1.4 --- Medium for Bacterial Culture --- p.18 / Chapter 2.1.5 --- Reagents for Preparation of Competent Cells --- p.19 / Chapter 2.2 --- DNA Isolation / Chapter 2.2.1 --- Extraction of DNA from meats --- p.20 / Chapter 2.2.2 --- Extraction of DNA from blood --- p.20 / Chapter 2.3 --- Phenol/Chloroform Extraction --- p.21 / Chapter 2.4 --- Ethanol Precipitation --- p.22 / Chapter 2.5 --- DNA Concentration/Purity Estimation --- p.22 / Chapter 2.6 --- Mitochondrial DNA amplification --- p.23 / Chapter 2.7 --- Random-Primed Polymerase Chain Reactions --- p.24 / Chapter 2.8 --- SCAR for Snake samples --- p.24 / Chapter 2.9 --- SCAR for Crocodile samples --- p.25 / Chapter 2.10 --- Restriction fragment length polymorphism analysis --- p.25 / Chapter 2.11 --- Agarose Gel Electrophoresis of DNA --- p.26 / Chapter 2.12 --- Purification of PCR product --- p.26 / Chapter 2.13 --- Preparation of Escherichia coli Competent Cells --- p.27 / Chapter 2.14 --- Ligation and transformation of E. coli --- p.27 / Chapter 2.15 --- Plasmid preparation --- p.28 / Chapter 2.16 --- Screening of Plasmid DNA by Restriction Digestion --- p.29 / Chapter Chapter 3 --- DNA sequencing of snakes & construction of snake database / Chapter 3.1 --- Introduction --- p.30 / Chapter 3.2 --- Materials and methods / Chapter 3.2.1 --- Snake samples --- p.32 / Chapter 3.2.2 --- "DNA Extraction, mitochondrial gene amplification and DNA sequencing" --- p.33 / Chapter 3.2.3 --- Construction of database --- p.33 / Chapter 3.3 --- Results / Chapter 3.3.1 --- Cytochrome b gene amplification and sequencing --- p.34 / Chapter 3.3.2 --- Gene amplification and sequencing of 16S rRNA --- p.42 / Chapter 3.3.3 --- Cytochrome b sequence database --- p.50 / Chapter 3.3.4 --- 16S rRNA sequence database --- p.53 / Chapter 3.4 --- Discussion / Chapter 3.4.1 --- Cytochrome b and 16S rRNA genes of snake species --- p.55 / Chapter 3.4.2 --- Cytochrome b and 16S rRNA databases --- p.55 / Chapter Chapter 4 --- Application of PCR-RFLP and SCAR in snake species identification / Chapter 4.1 --- Introduction --- p.57 / Chapter 4.2 --- Material and Methods / Chapter 4.2.1 --- DNA extraction and PCR-RFLP --- p.58 / Chapter 4.2.2 --- RAPD and SCAR --- p.58 / Chapter 4.3 --- Results / Chapter 4.3.1 --- PCR-RFLP of cytochrome b genes of snakes --- p.59 / Chapter 4.3.2 --- PCR-RFLP of 16S rDNA --- p.61 / Chapter 4.3.3 --- RAPD & SCAR analysis --- p.67 / Chapter 4.4 --- Discussion --- p.72 / Chapter Chapter 5 --- "Application of DNA sequencing, PCR-RFLP and SCAR to identify crocodile species" / Chapter 5.1 --- Introduction --- p.74 / Chapter 5.2 --- Materials and methods / Chapter 5.2.1 --- "Crocodile, human and four animal samples" --- p.75 / Chapter 5.2.2 --- "DNA Extraction, mitochondrial gene amplification and DNA sequencing" --- p.75 / Chapter 5.2.3 --- PCR-RFLP and SCAR --- p.76 / Chapter 5.3 --- Results / Chapter 5.3.1 --- Isolation of crocodiles DNA --- p.77 / Chapter 5.3.2 --- Isolation of DNA from Human and four animal species --- p.78 / Chapter 5.3.3 --- Cytochrome b gene amplification and sequencing --- p.78 / Chapter 5.3.4 --- 16S rRNA gene amplification and sequencing --- p.84 / Chapter 5.3.5 --- PCR-RFLP of cytochrome b --- p.89 / Chapter 5.3.6 --- PCR-RFLP of 16S rRNA --- p.91 / Chapter 5.3.7 --- SCAR primers for four crocodile species --- p.93 / Chapter 5.4 --- Discussion --- p.97 / Chapter Chapter 6 --- A case report - authentication of animal samples using DNA sequencing / Chapter 6.1 --- Introduction --- p.99 / Chapter 6.2 --- Material and methods / Chapter 6.2.1 --- Materials --- p.101 / Chapter 6.2.2 --- DNA Extraction and sequencing --- p.101 / Chapter 6.3 --- Result and discussion / Chapter 6.3.1 --- Cytochrome b gene sequencing --- p.102 / Chapter 6.3.2 --- Sequence homology among samples and meats obtained from the market --- p.111 / Chapter 6.3.3 --- Identity of samples B & D --- p.113 / Chapter Chapter 7 --- General Discussion / Chapter 7.1 --- Advantages and weakness of DNA technology --- p.116 / Chapter 7.2 --- Choosing appropriate molecular markers --- p.118 / Chapter 7.3 --- Further suggested work --- p.119 / Chapter 7.4 --- Conclusion --- p.119 / References --- p.121 / Appendix --- p.130
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Bases moléculaires du polymorphisme de compatibilité dans l'interaction Schistosoma mansoni / Biomphalaria glabrataMoné, Yves 15 March 2011 (has links) (PDF)
La dynamique co-évolutive qui joue dans les systèmes hôte-parasite conduit à une véritable course aux armements entre les deux protagonistes qui se traduit, dans certaines interactions comme celle qui est traitée dans cette thèse, par un polymorphisme de compatibilité dont les bases moléculaires sont méconnues. L'objectif de cette thèse était de progresser dans la connaissance des mécanismes moléculaires sous-jacents à ce polymorphisme de compatibilité dans l'interaction Biomphalaria glabrata/Schistosoma mansoni. Une approche protéomique comparative entre des souches de parasites compatibles et incompatibles nous a permis d'identifier des déterminants moléculaires clés de l'interaction exprimés par le parasite. Il s'agit d'une part de mucines hautement polymorphes potentiellement antigéniques, les "Schistosoma mansoni Polymorphic Mucin" (SmPoMucs), et d'autre part de molécules anti-oxydantes ("ROS scavengers"). Afin d'aborder la question de la course aux armements de manière complète, nous avons également recherché la "contre-partie moléculaire" exprimée par le mollusque et susceptible d'exprimer ce polymorphisme de compatibilité. Dans ce but, des approches de co-précipitation ont été menées. Elles ont permis de montrer que les SmPoMucs interagissaient avec des récepteurs immunitaires diversifiés du mollusque, les Fibrinogen-related Proteins (FREPs). Nous montrons ainsi pour la première fois dans une interaction parasite/hôte invertébré l'intervention d'un "système de type antigène-anticorps" impliquant un répertoire individuel polymorphe d'antigènes potentiels du parasite (les SmPoMucs) et un répertoire individuel diversifié de récepteurs immunitaires de son hôte (les FREPs). Nous avons également montré que le complexe immun formé par les deux dernières molécules citées incluait un troisième partenaire, une thioester-containing protein (TEP) qui appartient à une classe de molécules connue pour son rôle dans la fagocytose ou l'encapsulation. La présence de ce troisième partenaire au sein d'un même complexe renforce le rôle potentiellement immunitaire de ce complexe dans la reconnaissance et l'élimination du parasite. Au travers de cette thèse, nous nous sommes également intéressés à la course aux armements jouant sur les mécanismes effecteurs de l'immunité du mollusque. Dans notre modèle, les effecteurs responsables de la destruction du parasite sont principalement des espèces réactives de l'oxygène (ROS). Dans ce cas aussi, nous avons montré qu'il existe une concordance phénotypique entre la production de ROS par l'hôte et le niveau de "ROS scavengers" produits par le parasite pour contrecarrer la réaction de l'hôte. Ainsi, les mécanismes moléculaires responsables du polymorphisme de compatibilité dans l'interaction B. glabrata/S. mansoni s'appuieraient au moins sur deux facteurs d'une part sur la confrontation de répertoires de molécules polymorphes et/ou diversifiées en ce qui concerne les mécanismes de reconnaissance immunitaire, et d'autre part sur une adaptation réciproque quantitative en ce qui concerne certains mécanismes effecteurs de l'immunité.
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