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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
21

Viral Control of SR Protein Activity

Estmer Nilsson, Camilla January 2001 (has links)
<p>Viruses modulate biosynthetic machineries of the host cell for a rapid and efficient virus replication. One important way of modulating protein activity in eukaryotic cells is by reversible phosphorylation. In this thesis we have studied adenovirus and vaccinia virus, two DNA viruses with different replication stategies. Adenovirus replicates and assembles new virions in the nucleus, requiring the host cell transcription and splicing machinieries, whereas vaccinia virus replicates in the cytoplasm, only requiring the cellular translation machinery for its replication. </p><p>Adenovirus uses alternative RNA splicing to produce its proteins. We have shown that adenovirus takes over the cellular splicing machinery by modulating the activity of the essential cellular SR family of splicing factors. Vaccinia virus, that does not use RNA splicing, was shown to completely inactivate SR proteins as splicing regulatory factors. SR proteins are highly phosphorylated, a modification which is important for their activity as regulators of cellular pre-mRNA splicing. We have found that reversible phosphorylation of SR proteins is one mechanism to regulate alternative RNA splicing. We have demonstrated that adenovirus and vaccinia virus induce SR protein dephosphorylation, which inhibit their activity as splicing repressor and splicing activator proteins. We further showed that the adenovirus E4-ORF4 protein, which binds to the cellular protein phosphatase 2A, induced dephosphorylation of a specific SR protein, ASF/SF2, and that this mechanism was important for regulation of adenovirus alternative RNA splicing.</p><p>Inhibition of cellular pre-mRNA splicing results in a block in nuclear- to cytoplasmic transport of cellular mRNAs, ensuring free access of viral mRNAs to the translation machinery. We propose that SR protein dephosphorylation may be a general viral mechanism by which mammalian viruses take control over host cell gene expression.</p>
22

Regulation of adenovirus alternative pre-mRNA splicing : Functional characterization of exonic and intronic splicing enhancer elements

Yue, Bai-Gong January 2000 (has links)
<p>Pre-mRNA splicing and alternative pre-mRNA splicing are key regulatory steps controlling geneexpression in higher eukaryotes. The work in this thesis was focused on a characterization of thesignificance of exonic and intronic splicing enhancer elements for pre-mRNA splicing.</p><p>Previous studies have shown that removal of introns with weak and regulated splice sitesrequire a splicing enhancer for activity. Here we extended these studies by demonstrating thattwo "strong" constitutively active introns, the adenovirus 52,55K and the Drosophila Ftzintrons, are absolutely dependent on a downstream splicing enhancer for activity <i>in vitro</i>.</p><p>Two types splicing enhancers were shown to perform redundant functions as activators ofSplicing. Thus, SR protein binding to an exonic splicing enhancer element or U1 snRNP bindingto a downstream 5'splice site independently stimulated upstream intron removal. The datafurther showed that a 5'splice site was more effective and more versatile in activating splicing.Collectively the data suggest that a U1 enhancer is the prototypical enhancer element activatingsplicing of constitutively active introns.</p><p>Adenovirus IIIa pre-mRNA splicing is enhanced more than 200-fold in infected extracts. Themajor enhancer element responsible for this activation was shown to consist of the IIIa branchsite/polypyrimidne tract region. It functions as a Janus element and blocks splicing in extractsfrom uninfected cells while functioning as a splicing enhancer in the context of infected extracts.</p><p>Phosphorylated SR proteins are essential for pre-mRNA splicing. Large amount recombinantSR proteins are needed in splicing studies. A novel expression system was developed to expressphosphorylated, soluble and functionally active ASF/SF2 in <i>E. Coli</i>.</p>
23

Functional Characterization of the Cellular Protein p32 : A Protein Regulating Adenovirus Transcription and Splicing Through Targeting of Phosphorylation

Öhrmalm, Christina January 2006 (has links)
<p>Cellular processes involved in the conversion of the genetic information from DNA into a protein are often regulated by reversible phosphorylation reactions. By modulating the phosphorylated status of key proteins their activity can either be enhanced or repressed. In this thesis I have studied the significance of phosphorylation in the regulation of transcription and splicing using human adenovirus as a model system.</p><p>The results show that the activity of the cellular SR family of splicing enhancer or repressor proteins are reduced in adenovirus infected nuclear extracts by a virus-induced hypophosphorylation. The viral E4-ORF4 was shown to induce SR protein dephosphorylation by recruiting the cellular protein phosphatase PP2A. The E4-ORF4/PP2A complex was shown to relieve the SR protein-mediated repression of late virus-specific splicing and further activate alternative splicing in transiently transfected cells. Collectively, these results showed that alternative splicing, like many other biological processes, is regulated by reversible protein phosphorylation.</p><p>Similarly, the cellular p32 protein was shown to cause hypophosphorylation of the SR protein ASF/SF2 resulting in a reduced RNA binding capacity of ASF/SF2. This change in ASF/SF2 RNA binding also had a drastic effect on the function of ASF/SF2 as a regulatory protein affecting splice site choice. The cellular p32 protein and the viral E4-ORF4 protein both target the same cellular splicing factor, ASF/SF2. However, they regulate splicing by different mechanisms. E4-ORF4 recruits a phosphatase to dephosphorylate ASF/SF2, while p32 sequester ASF/SF2 in an inactive complex.</p><p>Further, we demonstrated that overexpression of p32 during a lytic infection suppressed transcription from the adenovirus major late transcription unit. p32 induced a selective repression of CAAT-box containing promoters indicating the involvement of the transcription factor CBF/NF-Y in this regulation. A further analysis showed that p32 caused a hyperphosphorylation of the CTD of RNA Pol II, which resulted in a significant reduction in the processivity of Pol II during the elongation phase of transcription.</p><p>In summary, we have shown that E4-ORF4 regulates the activity of splicing regulatory SR proteins, and that p32 regulates the activity of the SR protein ASF/SF2 in splicing and Pol II processivity during transcription elongation. Mechanistically, both E4-ORF4 and p32 appears to function by regulating the phosphorylated status of key cellular proteins involved in these processes.</p>
24

Regulation of adenovirus alternative pre-mRNA splicing : Functional characterization of exonic and intronic splicing enhancer elements

Yue, Bai-Gong January 2000 (has links)
Pre-mRNA splicing and alternative pre-mRNA splicing are key regulatory steps controlling geneexpression in higher eukaryotes. The work in this thesis was focused on a characterization of thesignificance of exonic and intronic splicing enhancer elements for pre-mRNA splicing. Previous studies have shown that removal of introns with weak and regulated splice sitesrequire a splicing enhancer for activity. Here we extended these studies by demonstrating thattwo "strong" constitutively active introns, the adenovirus 52,55K and the Drosophila Ftzintrons, are absolutely dependent on a downstream splicing enhancer for activity in vitro. Two types splicing enhancers were shown to perform redundant functions as activators ofSplicing. Thus, SR protein binding to an exonic splicing enhancer element or U1 snRNP bindingto a downstream 5'splice site independently stimulated upstream intron removal. The datafurther showed that a 5'splice site was more effective and more versatile in activating splicing.Collectively the data suggest that a U1 enhancer is the prototypical enhancer element activatingsplicing of constitutively active introns. Adenovirus IIIa pre-mRNA splicing is enhanced more than 200-fold in infected extracts. Themajor enhancer element responsible for this activation was shown to consist of the IIIa branchsite/polypyrimidne tract region. It functions as a Janus element and blocks splicing in extractsfrom uninfected cells while functioning as a splicing enhancer in the context of infected extracts. Phosphorylated SR proteins are essential for pre-mRNA splicing. Large amount recombinantSR proteins are needed in splicing studies. A novel expression system was developed to expressphosphorylated, soluble and functionally active ASF/SF2 in E. Coli.
25

Viral Control of SR Protein Activity

Estmer Nilsson, Camilla January 2001 (has links)
Viruses modulate biosynthetic machineries of the host cell for a rapid and efficient virus replication. One important way of modulating protein activity in eukaryotic cells is by reversible phosphorylation. In this thesis we have studied adenovirus and vaccinia virus, two DNA viruses with different replication stategies. Adenovirus replicates and assembles new virions in the nucleus, requiring the host cell transcription and splicing machinieries, whereas vaccinia virus replicates in the cytoplasm, only requiring the cellular translation machinery for its replication. Adenovirus uses alternative RNA splicing to produce its proteins. We have shown that adenovirus takes over the cellular splicing machinery by modulating the activity of the essential cellular SR family of splicing factors. Vaccinia virus, that does not use RNA splicing, was shown to completely inactivate SR proteins as splicing regulatory factors. SR proteins are highly phosphorylated, a modification which is important for their activity as regulators of cellular pre-mRNA splicing. We have found that reversible phosphorylation of SR proteins is one mechanism to regulate alternative RNA splicing. We have demonstrated that adenovirus and vaccinia virus induce SR protein dephosphorylation, which inhibit their activity as splicing repressor and splicing activator proteins. We further showed that the adenovirus E4-ORF4 protein, which binds to the cellular protein phosphatase 2A, induced dephosphorylation of a specific SR protein, ASF/SF2, and that this mechanism was important for regulation of adenovirus alternative RNA splicing. Inhibition of cellular pre-mRNA splicing results in a block in nuclear- to cytoplasmic transport of cellular mRNAs, ensuring free access of viral mRNAs to the translation machinery. We propose that SR protein dephosphorylation may be a general viral mechanism by which mammalian viruses take control over host cell gene expression.
26

Functional Characterization of the Cellular Protein p32 : A Protein Regulating Adenovirus Transcription and Splicing Through Targeting of Phosphorylation

Öhrmalm, Christina January 2006 (has links)
Cellular processes involved in the conversion of the genetic information from DNA into a protein are often regulated by reversible phosphorylation reactions. By modulating the phosphorylated status of key proteins their activity can either be enhanced or repressed. In this thesis I have studied the significance of phosphorylation in the regulation of transcription and splicing using human adenovirus as a model system. The results show that the activity of the cellular SR family of splicing enhancer or repressor proteins are reduced in adenovirus infected nuclear extracts by a virus-induced hypophosphorylation. The viral E4-ORF4 was shown to induce SR protein dephosphorylation by recruiting the cellular protein phosphatase PP2A. The E4-ORF4/PP2A complex was shown to relieve the SR protein-mediated repression of late virus-specific splicing and further activate alternative splicing in transiently transfected cells. Collectively, these results showed that alternative splicing, like many other biological processes, is regulated by reversible protein phosphorylation. Similarly, the cellular p32 protein was shown to cause hypophosphorylation of the SR protein ASF/SF2 resulting in a reduced RNA binding capacity of ASF/SF2. This change in ASF/SF2 RNA binding also had a drastic effect on the function of ASF/SF2 as a regulatory protein affecting splice site choice. The cellular p32 protein and the viral E4-ORF4 protein both target the same cellular splicing factor, ASF/SF2. However, they regulate splicing by different mechanisms. E4-ORF4 recruits a phosphatase to dephosphorylate ASF/SF2, while p32 sequester ASF/SF2 in an inactive complex. Further, we demonstrated that overexpression of p32 during a lytic infection suppressed transcription from the adenovirus major late transcription unit. p32 induced a selective repression of CAAT-box containing promoters indicating the involvement of the transcription factor CBF/NF-Y in this regulation. A further analysis showed that p32 caused a hyperphosphorylation of the CTD of RNA Pol II, which resulted in a significant reduction in the processivity of Pol II during the elongation phase of transcription. In summary, we have shown that E4-ORF4 regulates the activity of splicing regulatory SR proteins, and that p32 regulates the activity of the SR protein ASF/SF2 in splicing and Pol II processivity during transcription elongation. Mechanistically, both E4-ORF4 and p32 appears to function by regulating the phosphorylated status of key cellular proteins involved in these processes.
27

Obten??o de emuls?es asf?lticas convencionais e modificadas com argilas e nanoargilas

Torres, J?lia Cristina de Lima 05 August 2013 (has links)
Made available in DSpace on 2014-12-17T15:01:34Z (GMT). No. of bitstreams: 1 JuliaCLT_DISSERT.pdf: 2162032 bytes, checksum: 7f341c00df066e306597bec708b72f71 (MD5) Previous issue date: 2013-08-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The increasing demand for asphalt leads to the development of techniques that can improve the quality of products and increase the useful working life of pavements. Consequently, there is a growing application of asphalt emulsions, which are produced from a mixture of petroleum asphalt cement (CAP) with an aqueous phase. The main advantage of asphalt emulsions is its cold application, reducing energy costs. Conventional emulsions are obtained using asphalt, water, solvent, and additives. The modified asphalt emulsion is developed by adding a modifying agent to conventional emulsions. These modifiers can be natural fibers, waste polymers, nanomaterials. In this work modified asphalt emulsion were obtained using organoclays. First, it was prepared a conventional asphalt emulsion with the following mass proportion: 50% of 50/70 penetration grade CAP, 0.6% of additives and 3% of emulsifier, 20% of solvent and 26.4% of water. It was used bentonite and vermiculite (1% and 4%) to obtain the modified asphalt emulsion. Bentonite and vermiculite were added in its raw state and as an organoclay form and as an organoclay-acid form, resulting in 26 experimental runs. The methodology described by Qian et al. (2011), with modifications, was used to obtain the organoclay and the organoclay-acid form. infrared spectroscopy (IR)) were used to characterize the clays and nanoclays. The emulsions were prepared in a colloidal mill, using 30 minutes and 1 hour as mixing time. After, the emulsions were characterized. The following tests were performed, in accordance with the Brazilian specifications (DNER- 369/97): sieve analysis, Saybolt Furol viscosity, pH determination, density, settlement and storage stability, residue by evaporation, and penetration of residue. Finally, it can be concluded that the use of nanoclays as asphalt modifiers represent a viable alternative to the road paving industry / Com o aumento da demanda por asfaltos, faz-se necess?rio o desenvolvimento de t?cnicas que melhorem a qualidade e aumentem o tempo de vida ?til dos pavimentos. Com isso, cresce a aplica??o das emuls?es asf?lticas, que s?o produzidas a partir de uma mistura de Cimento Asf?ltico de Petr?leo (CAP) e uma fase aquosa, tendo como principal vantagem a redu??o de custos energ?ticos devido a sua aplica??o ser a frio. Neste trabalho foram obtidas emuls?es asf?lticas convencionais e modificadas. Para a obten??o de emuls?es asf?lticas convencionais foram utilizados os seguintes constituintes: asfalto, ?gua, solvente e aditivos. As emuls?es asf?lticas modificadas foram desenvolvidas a partir da adi??o de um agente modificante ?s emuls?es asf?lticas convencionais. Os agentes modificantes utilizados foram a bentonita e a vermiculita natural e modificadas com tensoativos. Primeiramente, preparou-se uma emuls?o asf?ltica convencional nas seguintes propor??es: 50% de CAP 50/70, 0,6% de aditivos e 3% de emulsificante em rela??o ? quantidade de asfalto, 20% de solvente e 26,4% de ?gua. Para a obten??o da emuls?o asf?ltica modificada, foi utilizada a bentonita e vermiculita como agente modificador em propor??es de 1% e 4 %. Os constituintes foram adicionados ao moinho coloidal por per?odos de 30 e 60 minutos. Foram realizados 26 ensaios, onde a bentonita e vermiculita foram adicionadas na sua forma natural, na forma organof?lica e na forma organof?lica em meio ?cido. Para a obten??o da argila organof?lica e organof?lica em meio ?cido utilizou-se o m?todo de Qian et al. (2011), com adapta??es. Para caracteriza??o das argilas e nanoargilas utilizou-se a t?cnica de espectroscopia no infravermelho (IV). Ap?s o per?odo de emulsifica??o, foram realizados os ensaios para caracteriza??o da emuls?o obtida. Os seguintes ensaios foram realizados, de acordo com as especifica??es t?cnicas (DNER-EM 369/97): peneiramento, viscosidade Saybolt Furol, pH, densidade, sedimenta??o, res?duo por evapora??o e penetra??o. Por fim, pode-se concluir que as emuls?es asf?lticas modificadas com nanoargilas apresentam-se como uma alternativa para o setor de pavimenta??o
28

Obten??o de novas emuls?es asf?lticas utilizando ?leo lubrificante usado ou contaminado (OLUC)

Bezerra, Petrucia Karine Santos de Brito 28 September 2015 (has links)
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-05-02T16:13:43Z No. of bitstreams: 1 PetruciaKarineSantosDeBritoBezerra_DISSERT.pdf: 1333218 bytes, checksum: 31616bff95f37e199ce03aa77c2f4a71 (MD5) / Approved for entry into archive by Monica Paiva (monicalpaiva@hotmail.com) on 2017-05-02T16:22:31Z (GMT) No. of bitstreams: 1 PetruciaKarineSantosDeBritoBezerra_DISSERT.pdf: 1333218 bytes, checksum: 31616bff95f37e199ce03aa77c2f4a71 (MD5) / Made available in DSpace on 2017-05-02T16:22:31Z (GMT). No. of bitstreams: 1 PetruciaKarineSantosDeBritoBezerra_DISSERT.pdf: 1333218 bytes, checksum: 31616bff95f37e199ce03aa77c2f4a71 (MD5) Previous issue date: 2015-09-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Emuls?es asf?lticas (EAs) s?o dispers?es de Cimento Asf?ltico de Petr?leo (CAP) em fase aquosa ou vice-versa, estabilizadas por tensoativos, produzidas em moinhos coloidais. Neste trabalho foram desenvolvidas e caracterizadas emuls?es asf?lticas convencionais (EACs) e emuls?es asf?lticas modificadas (EAMs) pela incorpora??o de ?leo lubrificante usado ou contaminado (OLUC). De acordo com a Resolu??o n? 362/2005 do CONAMA, todo o OLUC deve ser destinado ? reciclagem por meio do processo de rerrefino. Por?m, alguns fatores ligados ? infraestrutura da ind?stria de rerrefino, como a fiscaliza??o insuficiente, o baixo percentual de coleta (< 40%), o alto custo de log?stica, frente ao rendimento do processo, que ? em torno de 60%, t?m inviabilizado o rerrefino no Brasil. Sendo o OLUC quimicamente compat?vel com o CAP, o principal objetivo deste estudo foi incorpora-lo ao CAP para produzir emuls?es asf?lticas modificadas est?veis, de menor custo e com possibilidades de aplica??o em pavimentos sem danos ao meio ambiente. Planejamentos experimentais foram utilizados como ferramenta para direcionar o estudo e reduzir o n?mero e o tempo das etapas necess?rias ? obten??o e caracteriza??o das EAs. Inicialmente, realizou-se um estudo preliminar para definir a composi??o b?sica e as condi??es de emulsifica??o para obten??o EACs. No planejamento fatorial 1 (2?), avaliou-se os fatores temperatura de emulsifica??o, tempo de processo e quantidade de tensoativo na obten??o de EACs. No planejamento fatorial 2 (2?), verificou-se a influ?ncia das quantidades de CAP e tensoativo na obten??o de EACs, fixando as vari?veis temperatura (80?C) e tempo de emulsifica??o (5 min.) j? otimizadas no primeiro planejamento. No planejamento fatorial 3 (2n + 2.n + 4; n=2), fixou-se a vari?vel CAP em 60%, determinado no planejamento anterior, e estudou-se as quantidades de OLUC e tensoativo necess?rias ? obten??o de EAMs com caracter?sticas compat?veis ? Norma DNIT 165/2013. Todas as EAs obtidas nos planejamentos foram caracterizadas quanto ? viscosidade de Saybolt Furol (50?C), res?duo asf?ltico por evapora??o e sedimenta??o. Por fim, um estudo de otimiza??o das EAMs foi realizado na tentativa de enquadr?-las totalmente nas especifica??es do DNIT, caracterizando-as quanto ? viscosidade de Saybolt Furol (50?C), res?duo asf?ltico por evapora??o, sedimenta??o, peneira??o, penetra??o e ductilidade. Os resultados de caracteriza??o obtidos e avaliados estatisticamente mostraram que foi poss?vel produzir emuls?es asf?lticas est?veis e de acordo com a norma t?cnica espec?fica, operando em temperaturas n?o muito elevadas (80?C) por um curto per?odo de tempo (5 min.), com baixas quantidades de tensoativo. Concluindo-se que ? poss?vel incorporar o OLUC ?s EAs, obtendo-se EAMs com propriedades compat?veis ?s exig?ncias do ?rg?o regulamentador, com baixo custo e que podem ser usadas em opera??es de pavimenta??o. / Emuls?es asf?lticas (EAs) s?o dispers?es de Cimento Asf?ltico de Petr?leo (CAP) em fase aquosa ou vice-versa, estabilizadas por tensoativos, produzidas em moinhos coloidais. Neste trabalho foram desenvolvidas e caracterizadas emuls?es asf?lticas convencionais (EACs) e emuls?es asf?lticas modificadas (EAMs) pela incorpora??o de ?leo lubrificante usado ou contaminado (OLUC). De acordo com a Resolu??o n? 362/2005 do CONAMA, todo o OLUC deve ser destinado ? reciclagem por meio do processo de rerrefino. Por?m, alguns fatores ligados ? infraestrutura da ind?stria de rerrefino, como a fiscaliza??o insuficiente, o baixo percentual de coleta (< 40%), o alto custo de log?stica, frente ao rendimento do processo, que ? em torno de 60%, t?m inviabilizado o rerrefino no Brasil. Sendo o OLUC quimicamente compat?vel com o CAP, o principal objetivo deste estudo foi incorpora-lo ao CAP para produzir emuls?es asf?lticas modificadas est?veis, de menor custo e com possibilidades de aplica??o em pavimentos sem danos ao meio ambiente. Planejamentos experimentais foram utilizados como ferramenta para direcionar o estudo e reduzir o n?mero e o tempo das etapas necess?rias ? obten??o e caracteriza??o das EAs. Inicialmente, realizou-se um estudo preliminar para definir a composi??o b?sica e as condi??es de emulsifica??o para obten??o EACs. No planejamento fatorial 1 (2?), avaliou-se os fatores temperatura de emulsifica??o, tempo de processo e quantidade de tensoativo na obten??o de EACs. No planejamento fatorial 2 (2?), verificou-se a influ?ncia das quantidades de CAP e tensoativo na obten??o de EACs, fixando as vari?veis temperatura (80?C) e tempo de emulsifica??o (5 min.) j? otimizadas no primeiro planejamento. No planejamento fatorial 3 (2n + 2.n + 4; n=2), fixou-se a vari?vel CAP em 60%, determinado no planejamento anterior, e estudou-se as quantidades de OLUC e tensoativo necess?rias ? obten??o de EAMs com caracter?sticas compat?veis ? Norma DNIT 165/2013. Todas as EAs obtidas nos planejamentos foram caracterizadas quanto ? viscosidade de Saybolt Furol (50?C), res?duo asf?ltico por evapora??o e sedimenta??o. Por fim, um estudo de otimiza??o das EAMs foi realizado na tentativa de enquadr?-las totalmente nas especifica??es do DNIT, caracterizando-as quanto ? viscosidade de Saybolt Furol (50?C), res?duo asf?ltico por evapora??o, sedimenta??o, peneira??o, penetra??o e ductilidade. Os resultados de caracteriza??o obtidos e avaliados estatisticamente mostraram que foi poss?vel produzir emuls?es asf?lticas est?veis e de acordo com a norma t?cnica espec?fica, operando em temperaturas n?o muito elevadas (80?C) por um curto per?odo de tempo (5 min.), com baixas quantidades de tensoativo. Concluindo-se que ? poss?vel incorporar o OLUC ?s EAs, obtendo-se EAMs com propriedades compat?veis ?s exig?ncias do ?rg?o regulamentador, com baixo custo e que podem ser usadas em opera??es de pavimenta??o.
29

Särart och likhet, så vinner ASF legitimitet : En fallstudie om arbetsintegrerande sociala företags etablering. / Differentiation and similarity, how WISE gain legitimacy. : A case study on work integration social enterprises’ establishment in the field of work integration.

Alexandersson, Emma, Eriksson, Fanny January 2018 (has links)
The aim of this study was to examine how work integration social enterprise (WISE) established their enterprises. To reach the aim we collected data through qualitative in-terviews from six persons with experience of starting a WISE. Four managers, one exec-utive and one consultant. The research focused on their experience of the enterprises’ start up periods. The research shows that the enterprise is searching for legitimacy from its surrounding but in different ways depending who they are interacting with. The research presented in this study also shows four different factors important to WISE in the estab-lishment process; relations, needs, personal resources and the story about WISE.
30

“Vi är inte bara här för att uträtta jobbet utan vi är också här för vårt psykosociala mående.” : En kvalitativ studie av medarbetares upplevelser av att arbeta inom arbetsintegrerande sociala företag.

Meuller, Matilda, Karjalainen, Marielle January 2021 (has links)
The purpose of this study is to examine co-workers' experience of working within WISE and their perceived position in the company and at the labor market. The study also aims to investigate whether WISE contributes to perceived participation for co-workers, which is an ambition among these companies. The study answers three questions at issue; (1) How do co-workers within WISE perceive their work? (2) How do co-workers perceive their position and importance within the company and in the labor market? (3) How does work within WISE contribute to participation at the workplace? The study is based on a qualitative research method with an abductive approach. The questions at issue have been answered through eight semi-structured interviews with co-workers within two WISEs. The empirical material has then been analyzed on the basis of previous research linked to WISE and work, as well as our theoretical starting points, which are Empowerment, The work-first principle, Participation and SOC. The main results of the study shows that co-workers within the WISEs we have had contact with value work highly and as an important part of their lives. The work is described as meaningful as it provides the co-workers with a connection to colleagues and a place to be. The co-workers answers further indicate that they do not experience themselves as participants in an employment activity, but they see their position in the labor market as working individuals. By being active within a WISE, the co-workers have gained a sense of empowerment. The methods for how participation is achieved are similar between the two companies, where staff meetings are mentioned as the main strategy. The level of participation varies between co-workers, but what they all have in common is that they experience participation at the workplace. In this way, work within WISE have contributed to participation. / Studien har som syfte att undersöka medarbetarnas upplevelse av att arbeta inom ASF och deras upplevda ställning på företaget samt arbetsmarknaden. Studien syftar även till att undersöka huruvida ASF bidrar till upplevd delaktighet för medarbetarna, vilket är en ambition bland dessa företagen. Studien besvarar tre frågeställningar; (1) Hur upplever medarbetare inom ASF sitt arbete? (2) Hur upplever medarbetare sin position och betydelse inom företaget och på arbetsmarknaden? (3) Hur bidrar arbeten inom ASF till delaktighet på arbetsplatsen? Studien utgår från en kvalitativ forskningsmetod med en abduktiv ansats. Frågeställningarna har besvarats genom åtta semistrukturerade intervjuer med medarbetare inom två ASF. Det empiriska materialet har därefter analyserats utifrån tidigare forskning kopplat till ASF och arbete, samt våra teoretiska utgångspunkter vilka är Empowerment, Arbetslinjen, Delaktighet och KASAM. Studiens huvudresultat visar att medarbetare inom de ASF vi haft kontakt med värderar arbete högt och som en viktig del av deras liv. Arbetet beskrivs som meningsfullt då det tillför för medarbetarna ett sammanhang med kollegor och en plats att vara. Medarbetarnas svar tyder vidare på att de inte upplever sig som deltagare i en sysselsättningsverksamhet utan de ser sin position på arbetsmarknaden som arbetande individer. Genom att vara verksamma inom ASF har medarbetarna erhållit en känsla av empowerment. Metoderna för hur delaktighet uppnås är liknande mellan de två företagen där personalmöten nämns som den främsta strategin. Nivån av delaktighet varierar mellan medarbetarna men gemensamt är att de alla upplever delaktighet på arbetsplatsen. På så sätt har arbete inom ASF bidragit till delaktighet.

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