Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. A volume-regulated anion channel (VRAC) has been electrophysiologically characterized in innumerable mammalian cell types. VRAC is activated by cell swelling and mediates the volume regulatory efflux of Cl− and small organic solutes from cells. Two groups recently identified the mammalian leucine-rich repeat containing protein LRRC8A as an essential VRAC component. LRRC8A must be coexpressed with at least one of the other four members of this gene family, LRRC8B-E, to reconstitute VRAC activity in LRRC8−/− cells. LRRC8 genes likely arose with the origin of chordates. We identified LRRC8A and LRRC8C-E orthologs in the zebrafish genome and demonstrate that zebrafish embryo cells and differentiated adult cell types express a swelling-activated Cl− current indistinguishable from mammalian VRAC currents. Embryo cell VRAC currents are virtually eliminated by morpholino knockdown of the zebrafish LRRC8A ortholog lrrc8aa. VRAC activity is fully reconstituted in LRRC8−/− human cells by coexpression of zebrafish lrrc8aa and human LRRC8C cDNAs. lrrc8aa expression varies during zebrafish embryogenesis and lrrc8aa knockdown causes pericardial edema and defects in trunk elongation and somatogenesis. Our studies provide confirmation of the importance of LRRC8A in VRAC activity and establish the zebrafish as a model system for characterizing the molecular regulation and physiological roles of VRAC and LRRC8 proteins.

abstract: Humans are exposed up to thousands of per- and polyfluoroalkyl substances (PFAS) in the environment, but most of the research and action has been directed towards only two PFAS compounds. These two compounds are part of a subcategory of PFAS called perfluoroalkyl acids (PFAAs). It has been a challenge for the environmental community to mitigate risks caused by PFAAs due to their high persistence and lack of effective measures to remove them from the environment, especially in heavily impacted areas like fire-training sites. The goal of this work was to further answer some questions regarding the removal of PFAAs in the environment by looking at anion exchange resin characteristics and presence of a competing compound, natural organic matter (NOM), in the adsorption of environmentally relevant PFAS compounds including the two often monitored 8-carbon chain PFAAs. Two different resins were tested with two forms of counterions, in both groundwater and NOM impacted groundwater. Resin polymer matrix was the most important property in the adsorption of PFAAs, the two resins used A520E and A860 had similar properties except for their matrices polystyrene (PS) and polyacrylic (PA), respectively. The PS base is most effective at PFAAs adsorption, while the PA is most effective at NOM adsorption. The change in the counterion did not negatively affect the adsorption of PFAAs and is, therefore, a viable alternative for future studies that include regeneration and destruction of PFAAs. The presence of NOM also did not significantly affect the adsorption of PFAAs in the PS resin A520E, although for some PFAAs compounds it did affect adsorption for the PA resin. Ultimately, PS macroporous resins with a strong Type I or Type II base work best in PFAAs removal. / Dissertation/Thesis / Masters Thesis Engineering 2019

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京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第20551号 / 理博第4309号 / 新制||理||1619(附属図書館) / 京都大学大学院理学研究科化学専攻 / (主査)教授 北川 宏, 教授 竹腰 清乃理, 教授 吉村 一良 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

京都大学 / 新制・課程博士 / 博士(理学) / 甲第24435号 / 理博第4934号 / 新制||理||1705(附属図書館) / 京都大学大学院理学研究科化学専攻 / (主査)教授 依光 英樹, 教授 若宮 淳志, 教授 畠山 琢次 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

<p> A neutron activation analysis procedure for the simultaneous determination of Ru, Pd, Os, Ir, Pt and Au has been developed. It consists principally of distillation, anion exchange, solvent extraction and precipitation steps and was used to determine all of these metals in meteorites as well as Pd, Ir and Au in sulphide minerals from the Sudbury Nickel Irruptive. The accuracy of the method was checked against G-1 and W-1 and a sulphide standard. A literature survey of the geology of the Sudbury Nickel Irruptive and a description of the geology of the Strathcona mine are presented. A detailed description of the inorganic chemistr! and its bearing on the geochemistry of the precious metals, with special reference to basic rocks is given. Analytical results for Pd, Ir and Au in a large number of pyrrhotite and chalcopyrite samples and a smaller number of sulphide and oxide minerals and an ultramafic xenolith are presented. Arsenic was determined in 15 of the sulphide samples and found to be highly correlated with Pd. The genesis of the Strathcona ores is discussed and the differential behaviour of the precious metals during fractionation or a sulphide-rich basic magma is described. </p> <p> Seven carbonaceous and two enstatite chondrites were analysed and the data evaluated with respect to estimation of the cosmic abundances of these metals. </p> / Thesis / Doctor of Philosophy (PhD)

<p> An analysis of reported and theoretical methods of precipitating calcium carbonate from aqueous solution is presented, with emphasis on methods that generate the anion in the presence of Ca(++) ion.</p> <p> A new CaCO3 precipitation method referred to as precipitation from quasi-homogeneous solution and suitable for digital processor control is developed. This technique is then used to investigate the effects of ionic strength and Mg(++) ion on calcium carbonate nucleation.</p> <p> A minicomputer based interactive information system, capable of automatic data logging and processing of experimental results in studies using processor controlled precipitation of CaCO3 from quasi-homogeneous solution, is implemented and tested.</p> / Thesis / Master of Science (MSc)

京都大学 / 新制・課程博士 / 博士(工学) / 甲第24297号 / 工博第5070号 / 新制||工||1791(附属図書館) / 京都大学大学院工学研究科物質エネルギー化学専攻 / (主査)教授 陰山 洋, 教授 安部 武志, 教授 阿部 竜 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM

No description available.

No description available.