Large wind turbine blades, manufactured from fibre reinforced laminated composite materials, are key structural components of wind turbine systems. The demands for efficient and accurate modelling techniques of these composite blades have significantly increased. Over past decades, although complex computational models have been widely developed, more analytically based models are still very much desired to drive the design and optimization of these composite blades forward to be lighter, stronger, efficient and durable. The research work in this thesis aims to develop such more analytically based aerodynamic, structural and aero-elasticity models for large wind turbine blades manufactured from fibre reinforced laminated composite materials. Firstly, an improved blade element momentum (BEM) model has been developed by collectively integrating the individual corrections with the classic BEM model. Compared to other existing models, present BEM model accounts for blade tip and root losses more accurately. For laminar flow, the 3-D cross-flow is negligibly small. In this case, present BEM model with statically measured 2-D aerodynamic coefficients agrees closely to experimental measurements. However, stall delay correction is required for a 3-D rotating blade in stall. A new stall delay model is developed based on Snel s stall delay model. Verifications are performed and discussed for the extensively studied NREL UAE phase-VI test. The predictions of distributive and collective factors, e.g. normalised force coefficients, shaft torque and etc. have been compared to experimental measurements. The present BEM model and stall delay model are original and more accurate than existing models. Secondly, significant deficiency is discovered in the analytical thin-walled closed-section composite beam (TWCSCB) model proposed by Librescu and Vo, which is widely used by others for structural modelling of wind turbine blades. To correct such deficiency, an improved TWCSCB model is developed in a novel manner that is applicable to both single-cell and multi-cell closed sections made of arbitrary composite laminates. The present TWCSCB model has been validated for a variety of geometries and arbitrary laminate layups. The numerical verifications are also performed on a realistic wind turbine blade (NPS-100) for structural analysis. Consistently accurate correlations are found between present TWCSCB model and the ABAQUS finite element (FE) shell model. Finally, the static aero-elasticity model is developed by combining the developed BEM model and TWCSCB model. The interactions are accounted through an iterative process. The numerical applications are carried out on NPS-100 wind turbine. The numerical results show some significant corrections by modelling wind turbine blades with elastic coupling.

Methods of determining natural frequencies of the D76D88, B76D88, A86E93, C86G90, C86L90 and C125L89 turbine wheel designs for various environmental conditions were investigated by application of Finite Element Analysis and beam theory. Modelling and simulation methods were developed ; the first method composed of 15 finite element simulations ; the second composed of 15 finite element simulations and a set of experimental frequency survey results; the third composed of 5 simulations , an incorporated mathematical model and a set of experimental frequency survey results. Each of these methods was designed to allow prediction of resonant frequency changes across a range of exhaust gas temperature and shaft rotational speed. For the new modelling and simulation methods, an analysis template and a plotting tool were developed using Microsoft Excel and MATLAB software. A graph showing a frequency-temperature-speed variations and a Campbell Diagram that incorporates material stiffening and softening effects across a range of rotational speeds was designed, and applied to the D76D88, B76D88, A86E93, C86G90, C86L90 and C125L89 turbine wheel designs. New design methodologies for turbine wheels were formulated and validated, showing a good agreement with a range of data points from frequency survey, strain-gauge telemetry and laser tip-timing test results. The results from the new design method were compared with existing single compensation factor methodology, and showed a great improvement in accuracy of prediction of modal vibration. A new nomenclature for the mode shapes of a turbocharger’s blade was proposed, designed and demonstrated to allow direct identification of associated mode shape. It is concluded that Finite Element Analysis combined with the frequency survey is capable of predicting changes in turbine natural frequencies and, when incorporated into the existing turbine design methodology, resulted in a major improvement in the accuracy of the predictions of vibration frequency.

"A flow control method for minimizing losses in a highly loaded compressor blade was analyzed. Passive and active flow control experiments with vortex generator jets were conducted on a seven blade linear compressor cascade to demonstrate the potential application of passive flow control on a highly loaded blade. Passive flow control vortex generator jets use the pressure distribution generated by air flow over the blade profile to drive jets from the pressure side to the suction side. Active flow control was analyzed by pressuring the blade plenum with an auxiliary compressor unit. Active flow control decreased profile losses by approximately 37 % while passive flow control had negligible impact on the profile loss of a highly loaded blade. Passive flow control was able to achieve a jet velocity ratio, jet velocity to upstream velocity, of 0.525. The success of active flow control with a velocity ratio of 0.9 suggests there is potential for passive flow control to be effective. The research presented in this thesis is motivated by the potential savings in the applications of passive flow control in gas turbine axial compressors by increasing the aerodynamic load of each stage. Increased stage loading that is properly controlled can reduce the number of stages required to achieve the desired pressure compression ratio."

Introdução: O câncer de colo uterino é o quarto câncer mais comum no sexo feminino e um importante problema mundial de saúde pública. Recentemente, o uso de biomarcadores para melhorar a sensibilidade e especificidade do rastreamento e diagnóstico desta patologia passou a ter maior importância. Dentre estes, P16 e Ki67 passaram a ser largamente utilizados em imunohistoquímica de amostras preservadas em parafina. Entretanto, não se sabe qual a influência do tempo e temperatura de armazenamento de amostras previamente cortadas. Objetivo: O presente estudo tem por finalidade avaliar a influência do tempo de preparo e temperatura de armazenamento na imunorreatividade para P16 e Ki67 de cortes de amostras cervicais. Métodos: Amostras de blocos de parafina de câncer de colo uterino foram seccionadas e montadas em lâminas, de maneira seriada, no período de 9, 6, 3, 1 mês e tempo zero, sendo armazenadas em -20°C, 4°C e temperatura ambiente (TA). Todas as amostras então foram processadas ao mesmo tempo por imunohistoquímica para detecção de P16 e Ki67, sendo realizada leitura da mesma região do tumor nas diferentes condições. Resultados: Dos 10 casos de câncer de colo uterino, foram analisadas 75 regiões para P16 e Ki67 nas diferentes condições. A expressão de P16 e Ki67 não variou de maneira significativa ao longo do tempo nas diferentes condições de temperatura de armazenamento. Por exemplo, os cortes de 9 meses apresentaram a seguinte expressão quando armazenados a -20°C, 4°C e TA, respectivamente [mediana (p25-75)]: marcador P16 - 200 (160-300), 200 (180-300) e 200 (170-300), o que não foi estatisticamente diferente do corte em tempo zero, 200 (200-300), P=0,210; marcador Ki 67 - 210 (160-270), 210 (160-270) e 210 (145-270), o que também não foi estatisticamente diferente do corte em tempo zero, 240 (180-270), P=0,651. Conclusão: Não há influência significativa do tempo de preparo e temperatura de armazenamento de lâminas com material já cortado para a realização de imunohistoquímica posteriormente, no período de até 9 meses, para P16 e Ki67. Isto permite que, ao processarmos lâminas para HE e/ou outros marcadores, podemos reservar lâminas para posterior processamento com P16 e Ki67 sem prejuízo à imunorreatividade. / Introduction: Cervical cancer is the fourth most common cancer in women and a major public health problem in the world. Recently, the use of biomarkers to improve the sensitivity and specificity of the screening and diagnosis of this pathology has become more important. Among these, P16 and Ki67 became widely used in immunohistochemistry of samples preserved in paraffin. However, the influence of storage time and temperature of previously cut samples is not known. Aim: The aim of this study was to evaluate the influence of preparation time and storage temperature on the immunoreactivity for P16 and Ki67 of cervical specimens. Methods: Cervical cancer paraffin blocks were sectioned and mounted onto glass slides in 9, 6, 3, 1 month and zero time and stored at -20°C, 4°C and room temperature (RT). All slides were then processed at the same time by immunohistochemistry for the detection of P16 and Ki67, and the same tumor region was read under the different conditions. Results: Of the 10 cases of cervical cancer, 75 regions were analyzed for P16 and Ki67 under different conditions. Expression of P16 and Ki67 did not vary significantly over time at different storage temperature conditions. For example, the 9-month slides showed the following expression when stored at -20°C, 4°C and RT, respectively [median (p25-75)]: P16 - 200 (160-300), 200 (180-300) and 200 (170-300), which was not statistically different from zero time cut, 200 (200-300), P = 0.210; Ki 67 - 270 (160-270), 210 (160-270) and 210 (145-270), which was also not statistically different from zero-time cutoff, 240 (180-270), P = 0.651. Conclusion: There is no significant influence of the preparation time and storage temperature of slides of cervical cancer to be processed by immunohistochemistry later, in the period of up to 9 months, for P16 and Ki67. This allows, when processing slides for HE and / or other markers, we can reserve slides for further processing with P16 and Ki67 without impairing immunoreactivity.

No presente trabalho realizamos uma análise das lâminas líticas polidas produzidas pelos habitantes pré-históricos no território brasileiro, provenientes de diferentes regiões, grupos e modos de vida. A partir do estudo de coleções arqueológicas e de experimentações pretendemos compreender melhor as diferenças e semelhanças apresentadas por estas lâminas polidas em cada região e sua resistência a testes de impacto em laboratório. Foram analisados 926 artefatos, entre lâminas, fragmentos e alguns percutores. São peças que integram coleções de museus centrais em diferentes regiões do Brasil (Norte, Nordeste, Sudeste e Sul). São principalmente coleções antigas, que apresentam objetos provenientes de diversas partes de cada um dos estados, possibilitando uma cobertura territorial ampla. A despeito dos parcos dados de procedência das peças foi possível caracterizálas regionalmente de forma bastante clara, demonstrando que morfológica e tecnologicamente há variações significativas de uma região para a outra. Em alguns casos foi possível ir ainda mais longe, permitindo propor atribuições culturais a determinados tipos, relacionando-os a algumas Tradições Arqueológicas. No entanto, se tornou evidente também que a partilha dos modos de fazer regionais tem importância capital, que quase sempre vai além das escolhas culturais de um grupo específico. Os trabalhos experimentais (de compressão e impacto) evidenciaram a força necessária para a fragmentação de uma lâmina de basalto de tamanho intermediário, durante seu uso no corte, bem como permitiu estimar a força utilizada por um lenhador mediano, possibilitando compreender melhor algumas relações entre força de impacto, quebras e formas de encabamentos. Finalmente, conseguimos lançar luz sobre a importância das lâminas, tanto para as sociedades do passado, quanto para os arqueólogos do presente, mostrando-as como ferramentas importantes para a compreensão dos modos de vida e dos processos de ocupação do território. / In the present work, we performed an analysis of lithic polished blades produced by the prehistoric inhabitants of the Brazilian territory, from different regions, groups and ways of life. From the study of archaeological assemblages and of experimental archaeology we aim to better understand the differences and similarities presented by these polished blades in each region and their resistance to impact tests. We analyzed 926 artifacts, including blades, fragments and some hammerstones. These items integrate collections of central museums in different regions of Brazil (North, Northeast, Southeast and South). The collections were formed mostly from the middle of the XX century on and present objects from different parts of each of the states, making possible a wide territorial coverage. Despite the scarce data provenance for the pieces it was possible to characterize them regionally quite clearly, showing that morphologically and technologically there are significant variations from one region to another. In some cases it was possible to go even further, allowing the proposition of some cultural attributions to certain types of artifacts, relating them to some Archaeological Traditions. However, it also became clear that sharing the ways of doing things has a major role importance, which often goes beyond the cultural choices of a specific group. The experimental work (of compression and impact) revealed the force required to break a basalt blade of average size during its use in chopping, and allowing us to estimate the force used by an average lumberjack, then enabling a better understanding of some relationships between impact force, breakages and forms of hafting. Finally, we shed light on the importance of the blades, both for past societies, as to the present day archaeologists, showing them as important tools for understanding the ways of life and the processes involved in the human occupation of the territory.

The success of harnessing energy from ocean current will require a reliable structural design of turbine blade that is used for energy extraction. In this study we are particularly focusing on the fatigue life of a 3m length ocean current turbine blade. The blade consists of sandwich construction having polymeric foam as core, and carbon/epoxy as face sheet. Repetitive loads (Fatigue) on the blade have been formulated from the randomness of the ocean current associated with turbulence and also from velocity shear. These varying forces will cause a cyclic variation of bending and shear stresses subjecting to the blade to fatigue. Rainflow Counting algorithm has been used to count the number of cycles within a specific mean and amplitude that will act on the blade from random loading data. Finite Element code ANSYS has been used to develop an S-N diagram with a frequency of 1 Hz and loading ratio 0.1 Number of specific load cycles from Rainflow Counting in conjunction with S-N diagram from ANSYS has been utilized to calculate fatigue damage up to 30 years by Palmgren-Miner's linear hypothesis. / by Mohammad Wasim Akram. / Thesis (M.S.C.S.)--Florida Atlantic University, 2010. / Includes bibliography. / Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.

An experimental study on the application of Digital Image Correlation (DIC) to measure the deformation and strain of rotating blades is described. Commercial DIC software was used to obtain measurements on three different types of rotors with diameter ranging from 18 to 39 and with varying flexibility to explore applicability of the technique over a breadth of scales. The image acquisition was synchronized with the frequency of rotation such that images could be obtained at the same phase and the consistency of measurements was observed. Bending and twist distributions were extracted from the data with deformation as high as 0.4 measured with a theoretical accuracy of 0.0038 and span-wise resolution of 0.066. The technique was demonstrated to have many advantages including full-field high resolution results, non-intrusive measurement, and good accuracy over a range of scales. The span-wise deformation profiles from the DIC technique are used in conjunction with Blade Element Momentum Theory to calculate the thrust and power consumed by the rotor with rigid vi blades; results are comparable to load cell measurements albeit thrust is somewhat under-predicted and power is over-predicted. Overall, the correlation between DIC calculated thrust and BEMT approximations for comparable blades with constant pitch were within 12% through the onset of stall. Measurement of flexible blade deformation that would not have been possible with other techniques demonstrated the utility of the DIC method and helped to confirm predictions of flexible blade behavior. / text

Att ha statistiskt säkerställda mått på slitstyrkan hos precisionsverktyg så som tryckeriraklar kan användas för att underlätta vidare utveckling samt marknadsföring av dessa produkter. I följande examensarbete har utveckling av en metod för att studera slitstyrkan hos tryckeriraklar genomförts. Studierna har genomförts i en testrigg som simulera en rotogravyrtryckeripress. Examensarbetets mål är att utveckla en metod för att testa slitstyrkan av olika rakelprodukter i testriggen samt att tillämpa denna metod för att få dokumenterade jämförande tester mellan fem olika produkttyper. Resultaten av testerna visar nötningen av rakelbladen med lamellängdförlust och viktförlust som mått på nötningen. Det resultat som definitivt kan konstateras är att Produkt C visar lägre slitage än Produkt A. Lamellängdförlust rekommenderas som mått på slitaget under fortsatta studier för att underlätta jämförelser mellan blad med och utan ytbeläggning. För att erhålla högre repeterbarhet rekommenderas fortsatta studier med avseende på färgens korrosivitet, temperatur och viskositet. Vidare studier rekommenderas även för olika färgtyper, då med mjukare pigment och längre testlängd för att se huruvida det ökar repeterbarheten. Vidare studier bör genomföras med avseende på de olika nötningsmekanismer som rakelbladen utsätts för under testerna. / Having statistically acquired measurements on the wear of precision tools like doctor blades can be used to assist further development and marketing of these products. In this thesis the development of a testing method for studying the wear on doctor blades has been conducted. The tests have been performed in a testing rig to simulate a rotogravure printing press. The goal of the thesis work is to develop a method for testing the wear resistance of five different doctor blade products in a testing rig and then applying this method to get a documented comparative study between the different doctor blade products. The results from the testing shows wear of the doctor blades using lamella length loss and weight loss and units of measurement.The results clearly show that Product C shows less wear than Product A. Lamella length loss is the recommended unit of measurement for measuring the wear on the doctor blades, this makes it possible to compare coated and uncoated blades. For a higher repeatability of the tests further studies are recommended with the paints corrosiveness, temperature and viscosity in mind. Further studies are also recommended into different kinds of paint, with softer pigments while increasing the test length. Further studies should also be performed to gain a greater understanding of the different wear mechanisms that contribute to the wear of the doctor blades during testing.

Introdução: O câncer de colo uterino é o quarto câncer mais comum no sexo feminino e um importante problema mundial de saúde pública. Recentemente, o uso de biomarcadores para melhorar a sensibilidade e especificidade do rastreamento e diagnóstico desta patologia passou a ter maior importância. Dentre estes, P16 e Ki67 passaram a ser largamente utilizados em imunohistoquímica de amostras preservadas em parafina. Entretanto, não se sabe qual a influência do tempo e temperatura de armazenamento de amostras previamente cortadas. Objetivo: O presente estudo tem por finalidade avaliar a influência do tempo de preparo e temperatura de armazenamento na imunorreatividade para P16 e Ki67 de cortes de amostras cervicais. Métodos: Amostras de blocos de parafina de câncer de colo uterino foram seccionadas e montadas em lâminas, de maneira seriada, no período de 9, 6, 3, 1 mês e tempo zero, sendo armazenadas em -20°C, 4°C e temperatura ambiente (TA). Todas as amostras então foram processadas ao mesmo tempo por imunohistoquímica para detecção de P16 e Ki67, sendo realizada leitura da mesma região do tumor nas diferentes condições. Resultados: Dos 10 casos de câncer de colo uterino, foram analisadas 75 regiões para P16 e Ki67 nas diferentes condições. A expressão de P16 e Ki67 não variou de maneira significativa ao longo do tempo nas diferentes condições de temperatura de armazenamento. Por exemplo, os cortes de 9 meses apresentaram a seguinte expressão quando armazenados a -20°C, 4°C e TA, respectivamente [mediana (p25-75)]: marcador P16 - 200 (160-300), 200 (180-300) e 200 (170-300), o que não foi estatisticamente diferente do corte em tempo zero, 200 (200-300), P=0,210; marcador Ki 67 - 210 (160-270), 210 (160-270) e 210 (145-270), o que também não foi estatisticamente diferente do corte em tempo zero, 240 (180-270), P=0,651. Conclusão: Não há influência significativa do tempo de preparo e temperatura de armazenamento de lâminas com material já cortado para a realização de imunohistoquímica posteriormente, no período de até 9 meses, para P16 e Ki67. Isto permite que, ao processarmos lâminas para HE e/ou outros marcadores, podemos reservar lâminas para posterior processamento com P16 e Ki67 sem prejuízo à imunorreatividade. / Introduction: Cervical cancer is the fourth most common cancer in women and a major public health problem in the world. Recently, the use of biomarkers to improve the sensitivity and specificity of the screening and diagnosis of this pathology has become more important. Among these, P16 and Ki67 became widely used in immunohistochemistry of samples preserved in paraffin. However, the influence of storage time and temperature of previously cut samples is not known. Aim: The aim of this study was to evaluate the influence of preparation time and storage temperature on the immunoreactivity for P16 and Ki67 of cervical specimens. Methods: Cervical cancer paraffin blocks were sectioned and mounted onto glass slides in 9, 6, 3, 1 month and zero time and stored at -20°C, 4°C and room temperature (RT). All slides were then processed at the same time by immunohistochemistry for the detection of P16 and Ki67, and the same tumor region was read under the different conditions. Results: Of the 10 cases of cervical cancer, 75 regions were analyzed for P16 and Ki67 under different conditions. Expression of P16 and Ki67 did not vary significantly over time at different storage temperature conditions. For example, the 9-month slides showed the following expression when stored at -20°C, 4°C and RT, respectively [median (p25-75)]: P16 - 200 (160-300), 200 (180-300) and 200 (170-300), which was not statistically different from zero time cut, 200 (200-300), P = 0.210; Ki 67 - 270 (160-270), 210 (160-270) and 210 (145-270), which was also not statistically different from zero-time cutoff, 240 (180-270), P = 0.651. Conclusion: There is no significant influence of the preparation time and storage temperature of slides of cervical cancer to be processed by immunohistochemistry later, in the period of up to 9 months, for P16 and Ki67. This allows, when processing slides for HE and / or other markers, we can reserve slides for further processing with P16 and Ki67 without impairing immunoreactivity.

O câncer de colo de útero é o terceiro tipo de neoplasia mais comum em mulheres no estado do Rio de Janeiro, perdendo apenas para câncer de mama e pulmão. Não há informação disponível sobre a qualidade dos exames utilizados para prevenção do câncer do colo do útero nos municípios fluminenses. O trabalho teve como objetivo avaliar a distribuição geográfica das unidades laboratoriais e a cobertura de exames e a performance da rede de laboratórios de citopatologia do estado do Rio de Janeiro, segundo regiões mesopolitanas e municípios, no que diz respeito aos exames citopatológicos realizados no âmbito do SUS, considerando a qualidade, a normalização dos procedimentos e a gestão do laboratório e propor melhorias. A pesquisa usa um desenho de estudo do tipo transversal incluindo dados relativos ao funcionamento dos laboratórios que fazem exame papanicolaou no âmbito do SUS no estado do Rio de Janeiro. Trata-se de um estudo com dados secundários, já coletados para atender a ação de avaliação dos laboratórios de citopatologia no âmbito do SUS, nesse trabalho também é usada a abordagem ecológica para estimar a cobertura dos exames em relação à população-alvo do programa de rastreamento de câncer de colo de útero. A rede de laboratórios de citopatologia do estado do Rio de Janeiro não teve desempenho satisfatório. As regiões que tiveram laboratórios com melhor desempenho foram Baia da Ilha Grande e Baixada Litorânea e aquelas com laboratórios de pior desempenho foram Médio Paraíba e Noroeste. Os critérios avaliados com melhor desempenho foram da dimensão qualidade e o mais fraco desempenho foi observado para os critérios da dimensão normalização. A dimensão de gestão de laboratórios teve desempenho regular. Um relevante achado desse estudo foi a insuficiente qualidade da leitura de lâminas, inclusive procedimentos de releitura, que pode ser explicado pela suposta falta de capacitação dos profissionais em todo o processo exigido desde a identificação, fixação, e formas de encaminhamento do material até a chegada aos laboratórios. Com relação à cobertura, alguns municípios se aproximam do parâmetro (0,30) porém, embora este dado revele a capacidade da rede estadual do rio de Janeiro de ofertar exames, é preciso que ele seja analisado em conjunto com as situações de citologia anterior e tempo da citologia anterior para verificação da periodicidade da oferta e o melhor dimensionamento do alcance da população alvo assim como a abrangência da rede laboratorial de cada município. Conclusão: Os dados mostram repetição desnecessária de exames citopatológicos, o que implica custos injustificados e uma situação ainda mais deficitária de alcance das ações do que tem revelado o indicador razão. Ocorrendo principalmente em regiões com predominância de laboratórios privados. A expansão da cobertura com base na periodicidade recomendada do exame é relevante no quadro estadual encontrado e deve vir associada a iniciativas que garantam a qualidade no processo de coleta e análise do material, bem como a adequada capacitação dos profissionais para adoção de condutas recomendadas para as lesões identificadas. / Cancer of the cervix is the third most common type of cancer in women in the state of Rio de Janeiro, second only to breast cancer and lung. No information is available about the quality of tests used to prevent cancer of the cervix in municipalities of Rio de Janeiro. Objective: To evaluate the geographic distribution of laboratory units and the coverage and network performance tests of cytopathology laboratories in the state of Rio de Janeiro, according mesopolitanas regions and municipalities, with respect to Pap smears performed within SUS, considering the quality , standardization of procedures and laboratory management and propose improvements. The research uses a design of cross-sectional study including data concerning the operation of laboratories performing Pap smears within the SUS in the state of Rio de Janeiro. This is a study of secondary data already collected to meet the action assessment of cytopathology laboratories within the SUS, is also used in this study the ecological approach to estimate the coverage of the tests in relation to the target population of the program screening of cancer of the cervix. The network of cytopathology laboratories in the state of Rio de Janeiro did not perform satisfactorily. Regions that had laboratories that performed well were the Big Island and Coastal Lowlands and those with worse performance labs were Middle Paraíba and Northwest.The criteria evaluated were the best performing of the size and quality weakest performance was observed for the criteria of size normalization. The size of laboratory management had regular performance. An important finding of this study was insufficient to read quality blades, including rereading procedures, which can be explained by the supposed lack of professional training required in the whole process from identifying, securing, routing and forms the material until the arrival laboratories. With respect to coverage, some municipalities approach the parameter (0.30) but, although this figure reveals the ability of the state of Rio de Janeiro to offer examinations, he must be considered in conjunction with situations of previous cytology and time of cytology prior to checking the periodicity of supply and better scaling of the reach of the target population as well as the scope of the laboratory network in each city. The data show unnecessary repetition of Pap smears, which implies unnecessary costs and an even more deficient range of actions that the indicator has revealed razão. Ocorrendo especially in regions with predominantly private laboratories. The expansion of coverage based on the recommended frequency of examination is relevant under state and found to be associated with initiatives to ensure quality in process of collection and analysis of the material, as well as adequate training of professionals to adopt guidelines recommended for lesions identified.