The aim of this thesis is to assess the suitability of cold-formed rectangular hollow sections (RHS) for plastic design. The project involved an extensive range of tests on cold-formed Grade C350 and Grade C450 (DuraGal) RHS beams, joints and frames. A large number of finite element analyses was also carried out on models of RHS beams. The conclusion is that cold- formed RHS can be used in plastic design, but stricter element slenderness (b/t) limits and consideration of the connections, are required. Further research, particularly into the effect of axial compression on element slenderness limits, is required before changes to current design rules can be finalised. Bending tests were performed on cold-formed RHS to examine the web and flange slenderness required to maintain the plastic moment for a large enough rotation suitable for plastic design. The major conclusions of the beam tests were: (i) Some sections which are classified as Compact or Class 1 by current steel design specifications do not maintain plastic rotations considered sufficient for plastic design. (ii) The current design philosophy, in which flange and web slenderness limits are independent, is inappropriate. An interaction formula is required, and simple formulations are proposed for RHS. Connection tests were performed on various types of knee joints in RHS, suitable for the column - rafter connection in a portal frame. The connection types investigated were welded stiffened and unstiffened rigid knee connections, bolted plate knee joints, and welded and bolted internal sleeve knee joints, for use in RHS portal frames. The ability of the connections to act as plastic hinges in a portal frame was investigated. The most important finding of the joint tests was the unexpected fracture of the cold-formed welded connections under opening moment before significant plastic rotations occurred. The use of an internal sleeve moved the plastic hinge in the connection away from the connection centre- line thus eliminating the need for the weld between the RHS, or the RHS and the stiffening plate, to carry the majority of the load. The internal sleeve connections were capable of sustaining the plastic moment for large rotations considered suitable for plastic design. Tests on pinned-base portal frames were also performed. There were three separate tests, with two different ratios of vertical to horizontal point loads, simulating gravity and horizontal wind loads. Two grades of steel were used for comparison. The aims of the tests were to examine if a plastic collapse mechanism could form in a cold-formed RHS frame, and to investigate if plastic design was suitable for such frames. In each frame, two regions of highly concentrated curvature were observed before the onset of local buckling, which indicated the formation of plastic hinges and a plastic collapse mechanism. An advanced plastic zone structural analysis which accounted for second order effects, material non-linearity and member imperfections slightly overestimated the strength of the frames. The analysis slightly underestimated the deflections, and hence the magnitude of the second order effects. A second order plastic zone analysis, which did not account for the effects of structural imperfections, provided the best estimates of the strengths of the frames, but also underestimated the deflections. While cold-formed RHS did not satisfy the material ductility requirements specified for plastic design in some current steel design standards, plastic hinges and plastic collapse mechanisms formed. This suggests that the restriction on plastic design for cold-formed RHS based on insufficient material ductility is unnecessary, provided that the connections are suitable for plastic hinge formation, if required. A large number of finite element analyses were performed to simulate the bending tests summarised above, and to examine various parameters not studied in the experimental investigation. To simulate the experimental rotation capacity of the RHS beams, a sinusoidally varying longitudinal local imperfection was prescribed. The finite element analysis determined similar trends as observed experimentally, namely that the rotation capacity depended on both the web slenderness and flange slenderness, and that for a given section aspect ratio, the relationship between web slenderness and rotation capacity was non-linear. The main finding of the finite element study was that the size of the imperfections had an unexpectedly large influence on the rotation capacity. Larger imperfections were required in the more slender sections to simulate the experimental results. There should be further investigation into the effect of varying material properties on rotation capacity.

The aim of this thesis is to assess the suitability of cold-formed rectangular hollow sections (RHS) for plastic design. The project involved an extensive range of tests on cold-formed Grade C350 and Grade C450 (DuraGal) RHS beams, joints and frames. A large number of finite element analyses was also carried out on models of RHS beams. The conclusion is that cold- formed RHS can be used in plastic design, but stricter element slenderness (b/t) limits and consideration of the connections, are required. Further research, particularly into the effect of axial compression on element slenderness limits, is required before changes to current design rules can be finalised. Bending tests were performed on cold-formed RHS to examine the web and flange slenderness required to maintain the plastic moment for a large enough rotation suitable for plastic design. The major conclusions of the beam tests were: (i) Some sections which are classified as Compact or Class 1 by current steel design specifications do not maintain plastic rotations considered sufficient for plastic design. (ii) The current design philosophy, in which flange and web slenderness limits are independent, is inappropriate. An interaction formula is required, and simple formulations are proposed for RHS. Connection tests were performed on various types of knee joints in RHS, suitable for the column - rafter connection in a portal frame. The connection types investigated were welded stiffened and unstiffened rigid knee connections, bolted plate knee joints, and welded and bolted internal sleeve knee joints, for use in RHS portal frames. The ability of the connections to act as plastic hinges in a portal frame was investigated. The most important finding of the joint tests was the unexpected fracture of the cold-formed welded connections under opening moment before significant plastic rotations occurred. The use of an internal sleeve moved the plastic hinge in the connection away from the connection centre- line thus eliminating the need for the weld between the RHS, or the RHS and the stiffening plate, to carry the majority of the load. The internal sleeve connections were capable of sustaining the plastic moment for large rotations considered suitable for plastic design. Tests on pinned-base portal frames were also performed. There were three separate tests, with two different ratios of vertical to horizontal point loads, simulating gravity and horizontal wind loads. Two grades of steel were used for comparison. The aims of the tests were to examine if a plastic collapse mechanism could form in a cold-formed RHS frame, and to investigate if plastic design was suitable for such frames. In each frame, two regions of highly concentrated curvature were observed before the onset of local buckling, which indicated the formation of plastic hinges and a plastic collapse mechanism. An advanced plastic zone structural analysis which accounted for second order effects, material non-linearity and member imperfections slightly overestimated the strength of the frames. The analysis slightly underestimated the deflections, and hence the magnitude of the second order effects. A second order plastic zone analysis, which did not account for the effects of structural imperfections, provided the best estimates of the strengths of the frames, but also underestimated the deflections. While cold-formed RHS did not satisfy the material ductility requirements specified for plastic design in some current steel design standards, plastic hinges and plastic collapse mechanisms formed. This suggests that the restriction on plastic design for cold-formed RHS based on insufficient material ductility is unnecessary, provided that the connections are suitable for plastic hinge formation, if required. A large number of finite element analyses were performed to simulate the bending tests summarised above, and to examine various parameters not studied in the experimental investigation. To simulate the experimental rotation capacity of the RHS beams, a sinusoidally varying longitudinal local imperfection was prescribed. The finite element analysis determined similar trends as observed experimentally, namely that the rotation capacity depended on both the web slenderness and flange slenderness, and that for a given section aspect ratio, the relationship between web slenderness and rotation capacity was non-linear. The main finding of the finite element study was that the size of the imperfections had an unexpectedly large influence on the rotation capacity. Larger imperfections were required in the more slender sections to simulate the experimental results. There should be further investigation into the effect of varying material properties on rotation capacity.

The content of this thesis is the construction technology project construction hall for the production of veterinary drugs Tekro in the village Nová Dedina. In this work addresses the project site equipment, scheduling, technical regulations, inspection and test plan, a study of the main technological stages of the building.

The theme of the master´s theses is to design a wooden structure of city theater with internal steel construction. The concept of wooden structure is developed in two versions according to standard ČSN EN. The theses solve the static effect of the construction and design of individual parts and materials. Theater has dimensions 49.0 x 25.6 m, max. height of 12.5 meters. The construstion is devise to withstand a weight and applicability. The supporting structure of the roof is solved using by 13 arched plate girders. The second variant is designed as arched truss girders. The gable wall is glassed-in, glass envelopes support by wooden structure with columns and side runners. The work also includes solutions for joins and construction details. The structure was solved in the program RSTAB Dlubal 8. The assessment of the components was done using additional module TIMBER Pro. First Variant of plate girder was checking by manual report. The work includes drawings.

The nuclear pore complex (NPC) is perhaps the largest protein complex in the eukaryotic cell, and controls the movement of molecules across the nuclear envelope. The NPC is composed of up to 30 proteins termed nucleoporins (Nups), each grouped in different sub-complexes. The transmembrane ring sub-complex is composed of Nups responsible for anchoring the NPC to the nuclear envelope. Bioinformatic analysis has traced all major sub-complexes of the NPC back to the last eukaryotic common ancestor, meaning that the nuclear pore structure and function is conserved amongst all eukaryotes. In this study Arabidopsis T-DNA knockout lines for these genes were investigated to characterise gene function. Differences in plant growth and development were observed for the ndc1 knockout line compared to wild-type but gp210 plants showed no phenotypic differences. The double knockout line gp210 ndc1 was generated through crosses to observe plant response to the knockout of two anchoring-Nup genes. No synergistic affect from this double knockout was observed, suggesting that more, as yet unidentified Nups function the transmembrane ring in plants. The sensitivity to nuclear export inhibitor leptomycin B (LMB) was tested also for knockout lines, although growth sensitivity to the drug was not observed. Nucleocytoplasmic transport of knockout lines was measured in cells transformed by particle bombardment. To express fluorescent protein constructs actively transported through the NPC, localisation of protein determined the nucleocytoplasmic transport of the cell. The ndc1single knockout and the double knockout gp210 ndc1 exhibited decreased nuclear export. Further experiments in determining NDC1 localisation and identification of other Nups in the transmembrane ring sub-complex would bring a more comprehensive understanding to the plant NPC.