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1	A qualidade espacial na obra de Louis I. Kahn Lorentz, Rafael de Conti January 2016 (has links) Essa dissertação apresenta um estudo analítico sobre a obra do arquiteto Louis I. Kahn, desde o ponto de vista da sua qualidade espacial, compreendida esta no modo como ocorre a interação entre espaço e usuário. Foram selecionados três edifícios como estudos de caso capazes de representar a síntese evolutiva da concepção de espaço ao longo da obra de Kahn, os quais foram submetidos a uma análise empírica baseada na experiência espacial do observador em movimento, lançando mão de um conjunto de ferramentas no assim denominado método do observador. Os edifícios estudados viabilizam o desenvolvimento de análises que abrangem desde as primeiras obras de Kahn, na década de 1930, até o seu falecimento, em 1974. A análise realizada registra o desempenho dos edifícios em termos de sua legibilidade e funcionalidade, compreendidas como qualidades inerentes ao espaço e ao modo como este condiciona a experiência do usuário, relacionandoas à concepção espacial do edifício. Procura-se ampliar a compreensão dos resultados obtidos à luz da evolução da obra teórica do autor, traçando relações de causa e efeito entre esta e os fenômenos registrados na realidade concreta dos edifícios. O trabalho busca ainda ampliar a compreensão da relação da obra de Kahn com o contexto da produção arquitetônica de sua época, investigando as influências que recebe e as contribuições que oferece, especialmente no que tange à continuidade da arquitetura moderna. / The present dissertation is as an analytical study about the work of architect Louis I. Kahn, focusing on its spatial quality, as funded in the interaction between space and people in motion. Three buildings were selected as case studies in order to capture the evolutionary synthesis of space conception happened during Kahn’s career. Each building was submitted to an empirical scrutiny based upon the spatial experience of a moving observer, using a set of tools named as the “method of the observer”. The buildings investigated are put in relation with other Kahn’s buildings, so allowing the development of an analytical discourse embracing since his first works, in the 1930’s, until his death, in 1974. The analysis registers the performance of the buildings in terms of their legibility and their functionality, both understood as qualities inherent to space and the way they shape the user’s experience, so relating them to the building’s spatial conception. The dissertation intends to enlarge the understanding of the findings by considering the evolution of Kahn’s theoretical work, so bringing cause-effect connections between theory and the registered phenomena in the concrete reality of buildings. Eventually, this study aims to enlarge the understanding of the link between Kahn’s work and the architectural environment of the time, so exploring the influences he received and contributions he gave, especially concerning the evolution of modern architecture. Kahn, Louis I., 1901-1974 Arquitetos Arquitetura moderna Projeto arquitetônico Configuração espacial Spatial quality Method of the observer Yale University Art Galley Salk Institute Phillips Exeter Academy Library
2	A qualidade espacial na obra de Louis I. Kahn Lorentz, Rafael de Conti January 2016 (has links) Essa dissertação apresenta um estudo analítico sobre a obra do arquiteto Louis I. Kahn, desde o ponto de vista da sua qualidade espacial, compreendida esta no modo como ocorre a interação entre espaço e usuário. Foram selecionados três edifícios como estudos de caso capazes de representar a síntese evolutiva da concepção de espaço ao longo da obra de Kahn, os quais foram submetidos a uma análise empírica baseada na experiência espacial do observador em movimento, lançando mão de um conjunto de ferramentas no assim denominado método do observador. Os edifícios estudados viabilizam o desenvolvimento de análises que abrangem desde as primeiras obras de Kahn, na década de 1930, até o seu falecimento, em 1974. A análise realizada registra o desempenho dos edifícios em termos de sua legibilidade e funcionalidade, compreendidas como qualidades inerentes ao espaço e ao modo como este condiciona a experiência do usuário, relacionandoas à concepção espacial do edifício. Procura-se ampliar a compreensão dos resultados obtidos à luz da evolução da obra teórica do autor, traçando relações de causa e efeito entre esta e os fenômenos registrados na realidade concreta dos edifícios. O trabalho busca ainda ampliar a compreensão da relação da obra de Kahn com o contexto da produção arquitetônica de sua época, investigando as influências que recebe e as contribuições que oferece, especialmente no que tange à continuidade da arquitetura moderna. / The present dissertation is as an analytical study about the work of architect Louis I. Kahn, focusing on its spatial quality, as funded in the interaction between space and people in motion. Three buildings were selected as case studies in order to capture the evolutionary synthesis of space conception happened during Kahn’s career. Each building was submitted to an empirical scrutiny based upon the spatial experience of a moving observer, using a set of tools named as the “method of the observer”. The buildings investigated are put in relation with other Kahn’s buildings, so allowing the development of an analytical discourse embracing since his first works, in the 1930’s, until his death, in 1974. The analysis registers the performance of the buildings in terms of their legibility and their functionality, both understood as qualities inherent to space and the way they shape the user’s experience, so relating them to the building’s spatial conception. The dissertation intends to enlarge the understanding of the findings by considering the evolution of Kahn’s theoretical work, so bringing cause-effect connections between theory and the registered phenomena in the concrete reality of buildings. Eventually, this study aims to enlarge the understanding of the link between Kahn’s work and the architectural environment of the time, so exploring the influences he received and contributions he gave, especially concerning the evolution of modern architecture. Kahn, Louis I., 1901-1974 Arquitetos Arquitetura moderna Projeto arquitetônico Configuração espacial Spatial quality Method of the observer Yale University Art Galley Salk Institute Phillips Exeter Academy Library
3	A qualidade espacial na obra de Louis I. Kahn Lorentz, Rafael de Conti January 2016 (has links) Essa dissertação apresenta um estudo analítico sobre a obra do arquiteto Louis I. Kahn, desde o ponto de vista da sua qualidade espacial, compreendida esta no modo como ocorre a interação entre espaço e usuário. Foram selecionados três edifícios como estudos de caso capazes de representar a síntese evolutiva da concepção de espaço ao longo da obra de Kahn, os quais foram submetidos a uma análise empírica baseada na experiência espacial do observador em movimento, lançando mão de um conjunto de ferramentas no assim denominado método do observador. Os edifícios estudados viabilizam o desenvolvimento de análises que abrangem desde as primeiras obras de Kahn, na década de 1930, até o seu falecimento, em 1974. A análise realizada registra o desempenho dos edifícios em termos de sua legibilidade e funcionalidade, compreendidas como qualidades inerentes ao espaço e ao modo como este condiciona a experiência do usuário, relacionandoas à concepção espacial do edifício. Procura-se ampliar a compreensão dos resultados obtidos à luz da evolução da obra teórica do autor, traçando relações de causa e efeito entre esta e os fenômenos registrados na realidade concreta dos edifícios. O trabalho busca ainda ampliar a compreensão da relação da obra de Kahn com o contexto da produção arquitetônica de sua época, investigando as influências que recebe e as contribuições que oferece, especialmente no que tange à continuidade da arquitetura moderna. / The present dissertation is as an analytical study about the work of architect Louis I. Kahn, focusing on its spatial quality, as funded in the interaction between space and people in motion. Three buildings were selected as case studies in order to capture the evolutionary synthesis of space conception happened during Kahn’s career. Each building was submitted to an empirical scrutiny based upon the spatial experience of a moving observer, using a set of tools named as the “method of the observer”. The buildings investigated are put in relation with other Kahn’s buildings, so allowing the development of an analytical discourse embracing since his first works, in the 1930’s, until his death, in 1974. The analysis registers the performance of the buildings in terms of their legibility and their functionality, both understood as qualities inherent to space and the way they shape the user’s experience, so relating them to the building’s spatial conception. The dissertation intends to enlarge the understanding of the findings by considering the evolution of Kahn’s theoretical work, so bringing cause-effect connections between theory and the registered phenomena in the concrete reality of buildings. Eventually, this study aims to enlarge the understanding of the link between Kahn’s work and the architectural environment of the time, so exploring the influences he received and contributions he gave, especially concerning the evolution of modern architecture. Kahn, Louis I., 1901-1974 Arquitetos Arquitetura moderna Projeto arquitetônico Configuração espacial Spatial quality Method of the observer Yale University Art Galley Salk Institute Phillips Exeter Academy Library
4	Fighting polio : selling the gamma globulin field trials, 1950-1953 Mawdsley, Stephen Edward January 2012 (has links) No description available. 900
5	The Characterisation of Putative Nuclear Pore-Anchoring Proteins in Arabidopsis thaliana Collins, Patrick January 2013 (has links) The nuclear pore complex (NPC) is perhaps the largest protein complex in the eukaryotic cell, and controls the movement of molecules across the nuclear envelope. The NPC is composed of up to 30 proteins termed nucleoporins (Nups), each grouped in different sub-complexes. The transmembrane ring sub-complex is composed of Nups responsible for anchoring the NPC to the nuclear envelope. Bioinformatic analysis has traced all major sub-complexes of the NPC back to the last eukaryotic common ancestor, meaning that the nuclear pore structure and function is conserved amongst all eukaryotes. In this study Arabidopsis T-DNA knockout lines for these genes were investigated to characterise gene function. Differences in plant growth and development were observed for the ndc1 knockout line compared to wild-type but gp210 plants showed no phenotypic differences. The double knockout line gp210 ndc1 was generated through crosses to observe plant response to the knockout of two anchoring-Nup genes. No synergistic affect from this double knockout was observed, suggesting that more, as yet unidentified Nups function the transmembrane ring in plants. The sensitivity to nuclear export inhibitor leptomycin B (LMB) was tested also for knockout lines, although growth sensitivity to the drug was not observed. Nucleocytoplasmic transport of knockout lines was measured in cells transformed by particle bombardment. To express fluorescent protein constructs actively transported through the NPC, localisation of protein determined the nucleocytoplasmic transport of the cell. The ndc1single knockout and the double knockout gp210 ndc1 exhibited decreased nuclear export. Further experiments in determining NDC1 localisation and identification of other Nups in the transmembrane ring sub-complex would bring a more comprehensive understanding to the plant NPC. Arabidopsis thaliana transformation insert transient expression epidermal cells root cells gene protein plant bolting flowering root growth knockout ndc1 gp210 At1g73240 At5g40480 SALK SAIL Columbia seed germination DNA sequencing light microscopy stereo-fluorescence microscopy DNA extraction cell nucleus cytoplasm nucleoplasm nuclear pore complex nucleoporin nuclear envelope putative nuclear pore-anchoring protein leptomycin B gene gun particle bombardment agrobacterium T-DNA fasciation rhodococcus fascians cross reverse genetics nucleocytoplasmic transport cross self synergistic silique transmembrane ring dihybrid pumilo homology domain protein GFP YFP RFP PCR homozygote heterozygote inhibition

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