Yes / Background: Chronic infections with Pseudomonas aeruginosa are a leading cause of morbidity in patients with cystic fibrosis (CF). The aim of tobramycin inhalation therapy in CF patients with chronic pulmonary infection is to deliver high amounts of drug directly to the site of infection. TOBI® is a tobramycin nebulizer solution (300 mg/5 ml) approved by FDA for maintenance therapy for patient with CF. The 20% tobramycin sulfate solution was reported as the optimal and maximal concentration. Methods: Nebulization of high strength tobramycin solution (20% tobramycin sulfate) (HSTS) has been assessed in this study by using different selected high performance nebulizer delivery systems: two different designs of jet nebulizers, and three new nebulizers based on vibrating mesh technology. The aerosol particle size distribution and output characteristics were measured for in vitro performance assessment of the nebulizer systems. The methodology was adapted from the current European standard, EN 13544-1:2001E. Results: The particle size distribution characteristic measurements showed that all tested nebulizers may be suitable for inhalation of HSTS. The mean (SD) of highest percentage of fine particles (<5 mm) was 77.64 (2.3) % for Sidestream®, at flow rate 16 L/min. The highest respirable inhaled mass was for Pari LC Plus® combined with PariBoyN® compressor, with mean (SD) 90.85 (8.6) mg. The mean (SD) of highest drug wastage percentage was 63.9 (3.9) % for Sidestream® jet nebulizer combined with compressed air cylinder at flow rate 16 L/min, while the lowest was 2.3 (0.26) % for NE-U22 Omron® (high frequency). Conclusions: The HSTS can be nebulized by all tested nebulisers but the high frequency NE-U22 Omron® and Aeroneb Go® are more efficient. When the HSTS compared to TOBI®, the respirable inhaled dose was increased to more than 73%.

La localisation des ARNm au niveau des microtubules et des centrosomes laisse voir le centrosome et le fuseau mitotique comme des complexes ribonucléoprotéiques. Cependant, le mécanisme de localisation des ARNm à ces différentes structures ainsi que leurs fonctions dans la régulation de la mitose restent encore incompris. L’objectif était ici de caractériser des protéines de liaison à l’ARN (RNA Binding Proteins, RBPs) fonctionnellement impliquées dans la localisation des ARNm mitotiques chez la Drosophile et d’évaluer la conservation de la fonction de ces RBPs dans les cellules humaines. La déplétion de RBPs par RNAi générée dans des Drosophiles mutantes résulte en des phénotypes distincts de localisation anormale de l’ARNm centrosomique cen et en des défauts mitotiques différents selon le RBP ciblé, suggérant des fonctions différentes de ces RBPs. De plus, dans les jeunes embryons, les RBPs Bru-2 et Mask semblent être fonctionnellement importants pour la mitose via la régulation de l’ARNm cen, donnant un aperçu de la possible fonction mitotique de RBPs dans la régulation d’un ARN centrosomique. De plus, il a été observé dans un criblage d’immunofluorescence dans des cellules HeLa en métaphase que HNRNPUL1 colocalise au fuseau et aux centrosomes. HNRNPUL1 pourrait être impliqué dans la régulation de l’ARNm CDR2 (orthologue de cen) puisque la déplétion de l’orthologue de HNRNPUL1 dans la Drosophile, CG30122, résulte en une localisation anormale de l’ARNm centrosomique cen. / The localization of mRNA to microtubules and centrosomes has led to the suggestion that the centrosome and mitotic spindle are in fact ribonucleoprotein complexes. However, the mechanism of mRNA localization to those structures and its functional contribution in mitosis regulation remain poorly characterized. The objectives here were to identify RNA Binding Proteins (RBPs) involved in mitotic mRNA localization in Drosophila and to assess the conservation of the function of these RBPs in human cells. RNAi-mediated RBP depletion in Drosophila mutants leads to distinct phenotypes of abnormal localization of the centrosomal cen mRNA, and to different mitotic defects depending on the targeted RBP, suggesting different functions for these RBPs. Moreover, in young embryos, Bru-2 and Mask RBPs seem to be functionally important for mitosis through cen mRNA regulation, giving insight into a possible RBP mitotic function in regulating a centrosomal mRNA. In addition, data from an immunofluorescence screen on HeLa cells at metaphase suggests that HNRNPUL1 colocalizes to the spindle and centrosomes. HNRNPUL1 may be involved in the regulation of CDR2 mRNA (cen ortholog) because depletion of the HNRNPUL1 ortholog in flies, CG30122, disrupted cen mRNA localization.

Auf dem Gebiet der Veterinärmedizin wird in Deutschland die Desinfektionsmittelprüfung nach den Richtlinien der Deutschen Veterinärmedizinischen Gesellschaft e.V. (DVG) durchgeführt. Diese Richtlinien realisieren derzeit eine Viruzidieprüfung nur für den Bereich Tierhaltung. Daher wurde in dieser Arbeit eine praxisnahe Methodik zur Prüfung der viruziden Wirksamkeit von Desinfektionsmitteln für den Bereich der Lebensmittelproduktion und der tierärztlichen Praxis entwickelt. Neben dem Einsatz von zwei relevanten Prüfviren, erfolgte die Prüfung der viruziden Wirksamkeit anhand fünf verschiedener chemischer Grundsubstanzen. Um praxisähnliche Bedingungen zu simulieren, wurden unterschiedliche Belastungssubstanzen und Prüftemperaturen zur Testung herangezogen. Die gewonnenen Erkenntnisse können somit auf dem Gebiet der Viruzidieprüfung in zukünftige Neufassungen der DVG-Richtlinie berücksichtigt werden.

The main aim of this thesis was to describe and evaluate the price trend of poultry in selected countries of European Union during the period 2003 2013. The first part of the thesis is focused on theoretical knowledge - the definition of price, pricing terms, pricing methods, factors that influence the price level, the elasticity of demand, legislation and Common Agricultural Policy. It is followed by methodology. In this part procedures and formulas are used to achieve the aim. Practical part is the most important part of the thesis. It describes the evolution price of poultry in selected countries of European Union during the period 2003-2013. In this part is tested existence seasonal fluctuation of price and dependence prices on revenue population. There is also calculated elasticity of demand and prediction of the future price of poultry until 2016. The conclusion sums up results of practical parts.

Objective of this thesis was to describe and evaluate the development of milk prices in selected countries of the European Union and The Czech Republic. The theoretical part describes the definition of price, pricing, division of prices, factors affecting the consumption of milk, the connection between consumption, price and income. The thesis also analyzes the particularities of agricultural prices compared with prices in other sectors. It was drawn from literature of Czech and foreign authors. In the methodical part there are described statistical and economic calculation methods. The development of milk prices in each selected EU countries is described in the practical part. Analyzed countries were not chosen randomly. In this paper, there are selected three old states (Denmark, Italy, United Kingdom) and three new states (Lithuania, Poland and Slovakia) and of course the Czech Republic. Furthermore, the price development is assessed by means of price indexes, using analysis of longer time series. In the longer term series there is used the time series analysis, where a linear trend is modeled and a verification of the correctness of the predicted trend results is made. The time series is also examined from the viewpoint of seasonal fluctuations. The thesis is more interested in the national environment, efficiency of large milk producers. In the third part there is calculated the dependence of price development on income situation in each country and the impact of prices on consumption. Coefficients of income elasticity and elasticity of consumption were used. Price and income elasticity was compared in each country of the EU. The conclusion contains summary of results and performed discussion.

Auf dem Gebiet der Veterinärmedizin wird in Deutschland die Desinfektionsmittelprüfung nach den Richtlinien der Deutschen Veterinärmedizinischen Gesellschaft e.V. (DVG) durchgeführt. Diese Richtlinien realisieren derzeit eine Viruzidieprüfung nur für den Bereich Tierhaltung. Daher wurde in dieser Arbeit eine praxisnahe Methodik zur Prüfung der viruziden Wirksamkeit von Desinfektionsmitteln für den Bereich der Lebensmittelproduktion und der tierärztlichen Praxis entwickelt. Neben dem Einsatz von zwei relevanten Prüfviren, erfolgte die Prüfung der viruziden Wirksamkeit anhand fünf verschiedener chemischer Grundsubstanzen. Um praxisähnliche Bedingungen zu simulieren, wurden unterschiedliche Belastungssubstanzen und Prüftemperaturen zur Testung herangezogen. Die gewonnenen Erkenntnisse können somit auf dem Gebiet der Viruzidieprüfung in zukünftige Neufassungen der DVG-Richtlinie berücksichtigt werden.

PROTECTION OF COMPETITION - BLOCK EXEMPTIONS The main purpose of my thesis is to describe and analyze one aspect of a block exemption regulation for vertical agreements, namely resale price maintenance. The thesis is composed of six parts, one of them dealing with block exemptions in general and the rest of them focusing on resale price maintenance. Chapter one introduces the topic of block exemptions and explains the features of block exemptions that are common to all of them. Chapter two presents the definition of resale price maintenance and describes the difference between fixed, minimum, maximum and recommended prices. Chapter three describes economic theories, which have influence on legal treatment of resale price maintenance. The chapter is divided into three subchapters, whereas the first one explicates pro-competitive effects of resale price maintenance, the second one focuses on its anticompetitive effects and the third one summarizes the economic theories of impact of resale price maintenance. Chapter four examines the legal framework of resale price maintenance in European law. Firstly, it describes the treatment of fixed, minimum, recommended and maximum prices. Then it analyzes resale price maintenance as an object restriction and examines possible exemption under Article 101 (1) of...

The diploma thesis is devoted to the transfer pricing, particularly focusing on one of the transfer pricing methods: the transactional net margin method. In the theoretical part, the thesis deals with the transfer pricing legislation and demonstrates examples of how the profit transfer may occur. Further the thesis describes the transfer pricing methods and its use, mainly focusing on the transactional net margin method and its practical application. It deals with the profit level indicators and the comparability that must be followed when applying this method. In the practical part the thesis presents a Czech manufacturing company engaged in transactions with related parties. Using the functional and comparative analysis the compliance with the arm's length principle is determined.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-08-09T14:55:57Z No. of bitstreams: 1 TES_PAULA_CRISTINA_VISONA_COMPLETO.pdf: 16296138 bytes, checksum: 9e7d14a3df6448979a0bd7903506b8f7 (MD5) / Made available in DSpace on 2017-08-09T14:55:58Z (GMT). No. of bitstreams: 1 TES_PAULA_CRISTINA_VISONA_COMPLETO.pdf: 16296138 bytes, checksum: 9e7d14a3df6448979a0bd7903506b8f7 (MD5) Previous issue date: 2017-07-04 / This work offers a perspective on the identification of sociocultural trends. We align the emergence of these trends to the constituent factors of the social imaginary, building on Maffesoli's work regarding the relationship between the imaginary and everyday experiences. We articulate this perspective with other contributions, particularly regarding methodology, built by combining aspects of comprehensive sociology, interpretive anthropology, and dialogism. By means of this methodology we seek to identify the consequences of a specific socio-cultural trend called being-together-with, so as to reveal a number of its associated emerging elements, in order to employ scenario building. Such methodological perspectives posit an approach to being-together-with which allows for the understanding of its respective resultant social forms qua social trend: Creative Collectives. Therefore, we identify some of these social forms in the city of Porto Alegre qith an understanding that, as the first stage of the manifestation of a sociocultural tendency, they stimulate other manifestations aimed at the emergence of new trends, particularly those related to the emergence of the creative economy. / Neste trabalho apresentamos uma perspectiva de identifica??o de tend?ncias socioculturais. Alinhamos a emerg?ncia dessas tend?ncias a fatores constituintes do imagin?rio social, apoiando-nos amplamente na perspectiva apresentada por Maffesoli no que tange ? rela??o entre o imagin?rio e as viv?ncias cotidianas. Articulamos esta perspectiva a outros aportes, principalmente no que se refere ? metodologia, constru?da pelo cruzamento entre aspectos da sociologia compreensiva, da antropologia interpretativa, e do dialogismo. Por meio desta metodologia, buscamos identificar desdobramentos de uma tend?ncia sociocultural espec?fica - denominada estar-junto-com - visando relevar alguns elementos emergentes, a fim de colocar em pr?tica a tarefa de constru??o de cen?rios de futuro. Tais perspectivas metodol?gicas postulam uma abordagem de aproxima??o com o que reconhecemos como formas sociais decorrentes do estar-junto-com enquanto tend?ncia sociocultural: os Coletivos Criativos. Para tanto, identificamos algumas destas formas sociais na cidade de Porto Alegre, compreendendo que, como primeiro est?gio de manifesta??o de uma tend?ncia sociocultural, acabam por estimular outras manifesta??es, objetivando o surgimento de novas tend?ncias, especialmente aquelas relacionadas ? emerg?ncia da economia criativa.

劉績. / 論文(哲學碩士) -- 香港中文大學硏究院歷史學部, 1997. / 參考文獻: leaves 133-143. / Liu Ji. / 前言 --- p.1 / Chapter 第一章： --- 晚淸社會軍事化的討論 --- p.4 / Chapter 一 226}0Ø --- 理論與實踐的探討 --- p.5 / Chapter 二 226}0Ø --- 國家、社會與地方精英 --- p.8 / Chapter ´三Ø --- 資料來源 --- p.11 / Chapter 第二章： --- 岑毓英與廣西西林縣 --- p.15 / 廣西的社會經濟區 --- p.16 / Chapter ´二Ø --- 岑毓英的故鄉 --- p.25 / Chapter ´三Ø --- 小結 --- p.37 / Chapter 第三章： --- 由習文而練武一晚淸地方叛亂與 岑毓英的權力基礎 --- p.38 / Chapter 一 226}0Ø --- 岑毓英的抉擇 --- p.38 / Chapter ´二Ø --- 岑毓英權力的鞏固 --- p.41 / Chapter ´三Ø --- 小結 --- p.46 / Chapter 第四章： --- 回亂平定與岑毓英權力的建立 --- p.47 / Chapter 一 226}0Ø --- 與官方關係 --- p.47 / Chapter 二 226}0Ø --- 與地方軍事實力的關係 --- p.50 / Chapter ´三Ø --- 岑毓英平定回亂後的舉措 --- p.62 / Chapter ´四Ø --- 小結 --- p.66 / Chapter 第五章： --- 初任督撫的岑毓英 --- p.68 / Chapter 一 226}0Ø --- 軍事改革 --- p.68 / Chapter 二 226}0Ø --- 社會與財政改革 --- p.72 / Chapter ´三Ø --- 外交事務的挫敗 --- p.75 / Chapter ´四Ø --- 小結 --- p.79 / Chapter 第六章： --- 蟄伏的岑毓英 --- p.82 / Chapter ´一Ø --- 岑毓英與家族建設 --- p.83 / Chapter ´二Ø --- 復出政壇 --- p.93 / Chapter ´三Ø --- 小結 --- p.97 / Chapter 第七章： --- 中法戰爭前後的岑毓英 --- p.97 / Chapter 一 226}0Ø --- 岑毓英對法國帝國主義的反應 --- p.99 / Chapter 二 226}0Ø --- 內部的中法戰爭 --- p.104 / Chapter ´三Ø --- 中法戰爭與岑毓英 --- p.110 / Chapter ´四Ø --- 小結 --- p.112 / Chapter 第八章： --- 結論 --- p.115