Submitted by Irene Nascimento (irene.kessia@ufpe.br) on 2015-03-11T19:00:57Z No. of bitstreams: 2 DISSERTAÇÃO Fernanda de Oliveira Muniz.pdf: 1801971 bytes, checksum: 3e2686783d55d7dfb5f5cb1a7a2c71dc (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) / Made available in DSpace on 2015-03-11T19:00:57Z (GMT). No. of bitstreams: 2 DISSERTAÇÃO Fernanda de Oliveira Muniz.pdf: 1801971 bytes, checksum: 3e2686783d55d7dfb5f5cb1a7a2c71dc (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Previous issue date: 2013-12-06 / Atualmente, a economia necessita, cada vez mais, de melhores serviços e produtos frente a um mercado altamente competitivo. Para atender esta demanda e responder a essa alta competitividade, tem-se a inovação como um diferencial. Toda mudança ou criação de uma novidade carrega consigo risco e incerteza, visto ser a reação do mercado hipotética. Com base nesta situação, de risco e necessidade de inovar, surgiu a Mobilização Empresarial pela Inovação (MEI) cujo objetivo é subsidiar a inserção da cultura de inovação nas realidades das empresas brasileiras, através de projetos em todo o território nacional. Em Pernambuco, o projeto Núcleo de Gestão da Inovação (NUGI) é uma das primeiras iniciativas da MEI. Assim, o objetivo desta dissertação foi estudar o NUGI e a metodologia proposta pelos idealizadores da MEI, o MAPEL-R. Para alcançar tal objetivo, foi feita uma pesquisa bibliográfica sobre a importância da inovação e as características das empresas inovadoras, um estudo de caso sobre o NUGI e uma pesquisa de campo com as 04 (quatro) primeiras empresas participantes do projeto. A análise dos dados obtidos foi feita por meio de experiências práticas, estudo de caso e análises bibliográficas. Os resultados oriundos desta pesquisa mostram que a metodologia MAPEL-R e a “Empresa Inteligente” são complementares, tanto quanto à estrutura dos questionários, nos quais as qualidade e oportunidade de melhorias são integrantes, como também no que refere ao método de análise do grau de maturidade e ao potencial da empresa para a criação de valor, com a inovação.

Les inhibiteurs de la calcineurine (ICN) sont les immunosuppresseurs les plus employés en transplantation d’organe, malgré leur toxicité et leur efficacité imparfaite. Leurs effets présentent une large variabilité intra-et inter-individuelle, qui n’est pas expliquée par les différences de doses, de concentrations ou d’aires sous la courbe des concentrations en fonction du temps, ce qui limite les bénéfices du suivi thérapeutique pharmacologique et montre que d’autres facteurs contribuent à la variabilité de la réponse. Aucun biomarqueur unique actuellement disponible ne présente tous les prérequis idéaux, c'est -à-dire est à la fois non -invasif, fiable, sensible, spécifique, reproductible et disponible rapidement. Afin d’identifier des biomarqueurs pharmacodynamiques très spécifiques de l’inhibition de la calcineurine et reflétant une part importante de la variabilité inter-individuelle, nos travaux avaient pour objectifs d’explorer la pharmacodynamie des ICN, la force et la variabilité du signal le long de l’axe calcineurine, ainsi que les étapes où les sources de variabilité PD interne (génétiques) ou externe sont les plus influentes. Nos principaux résultats dans les PBMC de volontaires sains ex -vivo montrent que : l’inhibition de NFAT1 dans les noyaux de PBMC et celles de l’expression d’IL -2 et CD25 dans différentes sous -populations de lymphocytes T suivent des modèles I/Imax ; plusieurs polymorphismes dans les gènes impliqués dans la PD des ICN contribuent à la variabilité inter-individuelle de ces biomarqueurs. Chez des patients inscrits en liste d’attente de transplantation hépatique, nous avons pu : mesurer les biomarqueurs PD des ICN avant et après stimulation ex-vivo; montrer des relations PG/PK ainsi que PD/PD au sein de l’axe étudié. Chez des patients transplantés hépatique, ceux sous CsA avaient une plus grande variabilité inter-individuelle PD que ceux sous TAC, ainsi que différents types de régulations au sein de l’axe. En résumé, l’expression d’IL -2 et CD25 dans les lymphocytes T CD8+ ainsi que de CD25 dans les cellules T CD4+ pourraient être des biomarqueurs fiables de l’activité des ICN, qui intègrent la plus grande part de la variabilité inter-individuelle. De plus, des cas cliniques suggèrent que l’expression de NFAT1 dans les noyaux des PBMC pourrait aider à anticiper les épisodes d’infection, alors que la diminution des Treg et des niveaux élevés d’expression d’IL -2 dans les T CD8+ pourraient prédire la survenue du rejet cellulaire aigu. / Calcineurin inhibitors (CNI) are the immunosuppressants most employed in solid organ transplantation, despite their toxicity and suboptimal efficacy. Their effects show huge intra and inter-individual variability, not explained by differences in drug doses, concentrations or areas under the concentration -time curve, limiting the benefits of therapeutic drug monitoring and pointing that other factors contribute to response variability. No single biomarker currently available meets all the ideal requirements, i.e. non-invasiveness, reliability, sensitivity, specificity, reproducibility, and short turnaround time. To search for suitable PD biomarkers, i.e., with high specificity for calcineurin inhibition and most affected by inter-individual variability ,our works aimed at exploring the pharmacodynamics(PD) of CNI, the strength and variability of signal translation along the calcineurin pathway, as well as the steps where sources of internal (genetic) or external variability are the most influential .Our main results in healthy volunteers’ PBMC ex vivo showed : that the inhibition of NFAT1 in PBMC nuclei and of IL-2 and CD25 expression in different subsets of T lymphocytes followed I/Imax models; that IL-2 and CD25 responses to NFAT inhibition fitted and allosteric sigmoid model; and that several polymorphisms in genes involved in CNI PD participated in the inter-individual variability of these biomarkers. In patients on the waiting list of liver transplantation we were able: to measure CNI PD biomarkers before as well as after ex-vivo stimulation; to report PG/PD relationships, as well as PD/PD interactions within the pathway. In liver transplant recipients, those on cyclosporine showed more inter -individual PD variability than those on tacrolimus and different regulations within the pathway. In summary, IL-2 and CD25 in CD8+ T cells and CD25 in CD4+ T cells may be reliable biomarkers of CNI activity, with the largest inter-individual variability. Moreover , clinical cases suggest that NFAT1 levels in PBMC nuclei might help to anticipate infection episodes, while Tregs diminution and high levels of IL-2 expression in CD8+ T cells might predict acute cellular rejection.

Made available in DSpace on 2017-07-10T17:56:21Z (GMT). No. of bitstreams: 1 Suzane_Conceicao_Pantolfi_Tostes.PDF: 931248 bytes, checksum: b2e0058bed2703ced6887e7a6e3661f8 (MD5) Previous issue date: 2012-03-16 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The objective of this research is to investigate and analyze how Veja magazine made the cover over the role of class organizations (CUT, PT, FIESP e CNI) between the years 1985-1989. We assume that Veja magazine acts as a private apparatus of hegemony, since it proposes referrals, organize, create consensus and shares its world view with a certain fraction of the bourgeois class, in this period studied we concluded that Veja get closer of the fractions of the bourgeois class industrial business of the Brazil. The time frame of the research included the years 1985-1989, it was a remarkable period in Brazilian history, due to "end the military dictatorship" (but not the end of the rubble of authoritarian military dictatorship, once that, Constitution remained in force for the period of dictatorship until August 1988, the repression to the social movements and forms of mobilization and organization of the working class, the illegality of the strike, the permanence of the Ministry of Armed Forces and National Security all coordinated by the military), the promulgation of the New Constitution and with it the legality of the strike for some categories and the intensification of class struggle. Due to these factors the dissertation sought to discuss, analyze, investigate from the materials of the Veja magazine how it made the cover of the economic plans implemented during the years 1985 -1989 and the consequences for the working class. So as well as investigate the coverage that the magazine Veja made about the acting of the class organizations (CUT, PT, FIESP e CNI) in the period 1985-1989 and the clashes between these organizations / O objetivo desta pesquisa é investigar e analisar de que forma a revista Veja fez a cobertura sobre a atuação das organizações de classe (CUT, PT, FIESP e CNI) entre os anos de 1985-1989. Partimos do pressuposto que a revista Veja age enquanto um aparelho privado de hegemonia, já que propõe encaminhamentos, organiza, cria consenso e compartilha sua visão de mundo com uma determinada fração da classe burguesa, nesse período estudado concluímos que Veja se aproxima das frações da classe burguesa empresarial industrial do Brasil. O recorte temporal da pesquisa compreendeu os anos de 1985-1989, pois foi um período marcante na história brasileira, devido ao fim da ditadura militar (mas não o fim do entulho autoritário da ditadura militar, uma vez que, permaneceu vigente a Constituição do período da ditadura até agosto de 1988, a repressão aos movimentos sociais e formas de mobilização e organização da classe trabalhadora, ilegalidade da greve, permanência do Ministério das Forças Armadas e da Segurança Nacional todas coordenadas por militares), a promulgação da Nova Constituição e com ela a legalidade da greve para algumas categorias e a intensificação da luta de classes. Devido a esses elementos a pesquisa procurou discutir, analisar, investigar a partir das matérias da revista Veja, como a mesma fez a cobertura dos planos econômicos implantados durante os anos de 1985-1989 e as consequências para a classe trabalhadora, bem como investigar a cobertura que a revista Veja fez sobre a atuação das organizações de classe (CUT, PT, FIESP e CNI) no período de 1985-1989 e os embates entre essas organizações

The growth of distributed applications stand on a foundation of containers and their communication and have seen the rise and fall of many implementations throughout the years with a mix of proprietary and open sources. Today there are two implementations widely used as a result of the popularity of the huge project Kubernetes: CRI-O and Containerd. Both with the edge responsibility of managing containers using similar underlying software raising the question; do they have any implications on the containers they spawn? This thesis investigate these implementations from a performance perspective with a custom developed tool for direct communication to them and run a suite of benchmarks within the containers created by each. The suite consists of tests for throughput, latency, cpu, memory, random file read/write and sequential file read/write. Results conclude they perform similarly in all, but the file tests which showed overall CRI-O dominating in write speed and Containerd dominating the read speed.

Le caramel liquide, obtenu par le traitement thermique des sucres, est couramment utilisé pour modifier le goût ou la couleur des produits agroalimentaires.Parmi les promoteurs de caramélisation autorisés, l'ammoniaque (pour les caramels colorants de classe III) et les sels d'ammonium (pour ceux de classe IV) sont source de composés néoformés indésirables (CNI) comme le 2-acétyl-4-(1,2,3,4-tétrahydroxybutyl)imidazole (THI), immunosuppresseur, et le 4-méthylimidazole (4MeI), agent convulsif. Ces molécules ont des teneurs limitées par la réglementation Européenne à 10 et 250 ppm respectivement. Pour améliorer la connaissance de la chimie du caramel et la qualité des caramels colorants en contrôlant la formation des CNI, il est nécessaire de développer des méthodes analytiques fiables, rapides et faciles à mettre en œuvre. Ce travail de thèse présente le développement de nouvelles méthodes basées sur la chromatographie en phase liquide à deux-dimensions (LC-LC). Ces méthodes très sélectives permettent de minimiser le temps d'analyse puisque la préparation de l'échantillon se réduit à une simple dilution dans l'eau et les deux séparations chromatographiques en cascade sont réalisées sur un système totalement automatisé. La validation des méthodes par les profils d'exactitude a permis d'assurer que 90 % des résultats sont à moins de 20 % de la valeur vraie dans le domaine de concentration 10 à 50 ppm pour le THI, 20 à 500 ppm pour le 4MeI, 200 à 2500 ppm pour le fructose, saccharose, lactose, maltose et maltotriose et 500 à 2500 ppm pour le glucose.Ces méthodes sont enfin appliquées au suivi de la réaction de caramélisation et au contrôle qualité des produits finis.

Le caramel liquide, obtenu par le traitement thermique des sucres, est couramment utilisé pour modifier le goût ou la couleur des produits agroalimentaires.Parmi les promoteurs de caramélisation autorisés, l’ammoniaque (pour les caramels colorants de classe III) et les sels d’ammonium (pour ceux de classe IV) sont source de composés néoformés indésirables (CNI) comme le 2-acétyl-4-(1,2,3,4-tétrahydroxybutyl)imidazole (THI), immunosuppresseur, et le 4-méthylimidazole (4MeI), agent convulsif. Ces molécules ont des teneurs limitées par la réglementation Européenne à 10 et 250 ppm respectivement. Pour améliorer la connaissance de la chimie du caramel et la qualité des caramels colorants en contrôlant la formation des CNI, il est nécessaire de développer des méthodes analytiques fiables, rapides et faciles à mettre en œuvre. Ce travail de thèse présente le développement de nouvelles méthodes basées sur la chromatographie en phase liquide à deux-dimensions (LC-LC). Ces méthodes très sélectives permettent de minimiser le temps d’analyse puisque la préparation de l’échantillon se réduit à une simple dilution dans l’eau et les deux séparations chromatographiques en cascade sont réalisées sur un système totalement automatisé. La validation des méthodes par les profils d’exactitude a permis d’assurer que 90 % des résultats sont à moins de 20 % de la valeur vraie dans le domaine de concentration 10 à 50 ppm pour le THI, 20 à 500 ppm pour le 4MeI, 200 à 2500 ppm pour le fructose, saccharose, lactose, maltose et maltotriose et 500 à 2500 ppm pour le glucose.Ces méthodes sont enfin appliquées au suivi de la réaction de caramélisation et au contrôle qualité des produits finis. / Caramel liquid, obtained by heat treatment of sugars, is commonly used to alter the taste or colour of food products.Among promoters of caramelization allowed, ammonia (for caramel colours of class III) and ammonium salts (for those of class IV) are a source of neoformed contaminants (NFC) such as 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole (THI), immunosuppressive agent, and 4-methylimidazole (4MeI), convulsive agent. These molecules have contents limited by the European regulations to 10 and 250 ppm, respectively.To improve knowledge of the chemistry of caramel and quality of caramel colours by controlling the formation of NFC, it is necessary to develop analytical methods that are both reliable, fast and easy to implement. This thesis presents the development and validation of new methods based on two-dimensional liquid chromatography (LC-LC). These very selective methods can minimize analysis time since sample preparation is reduced to a simple dilution with water and the two consecutive chromatographic separations are performed on a fully automated system. The method validation by the accuracy profiles allowed to show that 90 % of obtained results correspond to the agreement value more or less 20 %, in the field of concentration 10 to 50 ppm for THI, 20 to 500 ppm for 4MeI, 200 to 2500 ppm for fructose, sucrose, lactose, maltose and maltotriose and 500 to 2500 ppm for glucose.These methods are finally applied to monitor the reaction of caramelization and to control the quality of finished products.

The telecommunications industry is rapidly advancing with the adoption of cloud-native technologies, aiming to enhance service delivery and network management. Kubernetes, an open-source platform for automating containerized applications, plays a significant role in this transformation. However, the use of Kubernetes in telecommunications presents unique challenges, particularly in effective network traffic separation. This thesis explores the feasibility and implications of exposing services on secondary network interfaces in Kubernetes to address traffic separation issues. The research investigates current trends and approaches for enabling service exposure on secondary interfaces, evaluates how these services support Kubernetes' resiliency features, and assesses the performance implications. A combination of literature review, empirical experiments, and interviews was used. Initially, a proof of concept (PoC) using Multus-service was attempted but faced setbacks due to the project's deactivation. Developer interviews revealed resistance within the Kubernetes SIG-Network group to modifying the established services API for secondary interfaces, and a lack of compelling use cases and community feedback led to the deprecation of Multus-service. Current trends indicate a shift towards more scalable, less disruptive solutions like the Gateway API. The focus then shifted to Meridio, another project claimed to have the capability of enabling service exposure through secondary interfaces. A successful PoC with Meridio in an OpenShift cluster served as the basis for further evaluations. The findings indicate that Meridio has the capability of providing service exposure through secondary network interfaces and aligns with Kubernetes' self-healing mechanisms. Performance evaluations showed that services on secondary interfaces could offer comparable overall performance to those on primary interfaces. Resource utilization metrics reveal additional CPU and memory overheads, but these are considered manageable. This research provides insights into the use of secondary network interfaces for service exposure in Kubernetes, contributing to ongoing discussions within Ericsson Cloud-RAN. The research underscores the need for further development and optimization, suggesting that with continued advancements, service exposure through secondary interfaces could enhance network management and service delivery in cloud-native environments.