L’étude précise des transitions ß de Fermi super-permises 0+ ? 0+ offre un outil précieux pour explorer les propriétés de l’interaction faible dans le cadre du Modèle Standard (SM). Collectivement, les forces (ft) mesurées pour ces transitions permettent de vérifier l’hypothèse CVC et contribuent au test le plus rigoureux de l’unitarité de la première ligne de la matrice de mélange des quarks CKM en fournissant l’évaluation la plus précise de l’élément dominant (Vud). Jusqu’à récemment, un apparent défaut d’unitarité a semé le doute sur la validité du SM minimal et a mobilisé un effort considérable afin d’élargir le champ d’étude à d’autres émetteurs de Fermi. Le 62Ga et 38Ca sont parmi des noyaux clés pour mener ces tests de précision au travers de la vérification de la fiabilité des corrections imposées aux valeurs ft expérimentales. La décroissance ß de 62Ga a été étudiée auprès du séparateur IGISOL à Jyväskylä, avec un dispositif composé de 3 Clovers EUROBALL pour la détection du rayonnement ?. La mise en évidence de raies ? de très faible intensité (<1‰), au travers de corrélations ß-? et ß-?-?, a permis de reconstruire partiellement le schéma de niveaux excités dans le 62Zn. Le rapport d’embranchement analogue déduit (B.R.A = 99.893(24) %) est utilisé pour extraire la valeur Ft(62Ga) universelle. Celle-ci s’avère en bon accord avec les 12 valeurs précises connues de la littérature. La compatibilité constatée entre la limite supérieure dressée ici sur le terme (d1IM) et la prédiction théorique confirme l’importance des corrections de brisure de symétrie d'isospin dans les émetteurs ß (A = 62). L’étude de la décroissance de 38Ca a été réalisée auprès de l’installation ISOLDE du CERN. L’utilisation de la fluoration des fragments de réaction, pour isoler chimiquement les isotopes d’intérêt, alliée au piégeage d’ions assisté par REXTRAP et l’analyse TOF, ont permis de s’affranchir totalement du contaminant contraignant 38mK. Pour la première fois, la durée de vie de 38Ca est mesurée avec un échantillon de haute pureté. Le résultat préliminaire établi T1/2(38Ca) = 445.8(10) ms améliore la précision par rapport à l’ancienne valeur d’un facteur proche de 10. / Precise measurements of Fermi superallowed 0+? 0+ ß decays provide a powerful tool to study the weak interaction properties in the framework of the Standard Model (SM). Collectively, the comparative half-lives (ƒt) of these transitions allow a sensitive probe of the CVC hypothesis and contribute to the most demanding test of the unitarity of the quarks-mixing CKM matrix top-row, by providing, so far, the most accurate determination of its dominant element (Vud). Until recently, an apparent departure from unity enhanced a doubt on the validity of the minimal SM and thus stimulated a considerable effort in order to extend the study to other Fermi emitters available. The 62Ga and 38Ca are among key nuclei to achieve these precision tests and verify the reliability of the corrections applied to the experimental ƒt-values. The 62Ga ß-decay was investigated at the IGISOL separator in Jyväskylä, with an experimental setup composed of 3 EUROBALL Clovers for ?-ray detection. Very weak intensity (<1‰) ?-rays were identified, via ß-? and ß-?-? correlations, and allowed a partial decay scheme reconstruction in 62Zn. The newly established analog branching-ratio (B.R.A = 99.893(24) %) was used to compute the universal Ft-value (62Ga). The latter turned out to be in good agreement with the 12 well-known cases. Compatibility between the upper limit set here on the term (d1IM) and the theoretical prediction suggests that the isospin-symmetry-breaking correction is indeed large for the heavy (A = 62) ß-emitters. The study of the 38a decay was performed at the CERN- ISOLDE facility. Injection of fluorine into the ion source, in order to chemically select the isotopes of interest, assisted by the REXTRAP Penning trap facility and a TOF analysis, enabled us to eliminate efficiently the troublesome 38mK. For the first time, the 38Ca half-life is measured with a highly purified radioactive sample. The preliminary result obtained, T1/2(38Ca) = 445.8(10) ms, improves the precision on the half-life as determined from previous measurements by a factor close to 10.

KPMG has recognized that large manufacturing corporates in Sweden are experiencing impaired growth possibilities and have therefore started to turn to startups to help with innovation. Corporates have traditionally focused on growth through product improvement or market expansion but have recently started to shift their focus to acquisitions and partnerships with high potential startups. However, since there are many differences between corporates and startups the collaboration can be difficult to handle, especially when it comes to integration. This study aims to investigate what types of startups large corporates are currently looking to collaborate with and to create a practical framework that can act as a guide to increase the chance of successfully integrating a startup into a corporate's operations. This study is a qualitative study that examines several Swedish manufacturing companies by delving into their specific cases. A thematic analysis, document and literature review is presented, and the results indicate that Swedish manufacturing corporates want startups with technologies that boost digital transformation (AI, IoT, digital twins) to increase predictability, traceability, and optimization. They also want to increase sustainability initiatives in terms of both technologies and materials. This is followed by a framework for how a successful corporate-startupintegration should be done. The results from the study contribute to the work of improving collaboration between startups and corporates and the results from this study can help create economic benefits, promote social sustainability, and address environmental challenges more effectively. / KPMG har uppmärksammat att stora tillverkningsföretag i Sverige upplever försämrade tillväxtmöjligheter och har därför börjat vända sig till startups för att få hjälp med innovation. Företag har traditionellt fokuserat på tillväxt genom produktförbättringar eller marknadsexpansion, men har nyligen börjat skifta fokus till förvärv och partnerskap med startups med hög potential. Då det finns stora skillnader mellan stora företag och startups kan samarbetet vara svårt att hantera, särskilt när det gäller integration. Denna studie syftar till att undersöka vilka typer av startups som stora företag vill samarbeta med och att skapa ett praktiskt ramverk som kan fungera som en guide för att öka chansen att framgångsrikt integrera ett startup i företagets verksamhet. Denna studie är en kvalitativ studie som undersöker flera svenska tillverkningsföretag. En tematisk analys, dokument- och litteraturgranskning presenteras, och resultaten indikerar att svenska tillverkningsföretag vill ha startups med teknik som främjar digital transformation (AI, IoT, digitala tvillingar) för att öka förutsägbarhet, spårbarhet och optimering. De vill också öka hållbarhetsinitiativen när det gäller både teknik och material. Detta följs av ett ramverk för hur en framgångsrik integration mellan företag och startups bör gå till. Resultaten från studien bidrar till arbetet med att förbättra samarbetet mellan startups och företag och resultaten från denna studie kan bidra till att skapa ekonomiska fördelar, främja social hållbarhet och hantera miljöutmaningar mer effektivt

Ce travail expérimental porte sur l'influence des paramètres de synthèse de nanotubes de carbone par CVD sur leurs caractéristiques structurales. Cette étude repose sur des analyses ex situ et in situ par spectroscopie Raman d'échantillons synthétisés sous diverses conditions de croissance. Une étude sur des films de cobalt oxydés a montré qu'il existe une variation de la pression seuil pour la croissance de nanotubes de carbone en fonction de l'épaisseur de cobalt que nous proposons d'attribuer à une pression seuil de réduction des particules.Une étude des bandes D et G' nous a permis d'attribuer les composantes basses fréquences aux nanotubes mono-feuillets et les composantes hautes fréquences à des espèces carbonées co-produites lors de la synthèse. Cette nouvelle approche a permis une étude plus fine des défauts et la proposition d'un modèle de création des défauts dans les nanotubes synthétisés par CVD. Enfin, une étude systématique des RBM nous a permis de mettre en évidence des processus favorisant la croissance de nanotubes mono-feuillets de petits ou de moyens diamètres. Ainsi, le contrôle de l'épaisseur de catalyseur, de la nature du précurseur carboné, de sa pression partielle ou encore de la température de synthèse permet de contrôler la distribution en diamètre des nanotubes mono-feuillets. / This experimental work deals with the influence of CVD growth parameters on carbon nanotube structural properties. The study is based on in situ and ex situ Raman spectroscopy on samples synthesized under various conditions of growth. A work on oxidized cobalt films showed that the threshold pressure for nanotube growth depends on the thickness of the cobalt film. We assign this pressure to a threshold pressure of reduction. A study of the D-band and G'-band allowed to assign low-frequency components to SWNT and high-frequency components to carbonaceous co-products of synthesis. This new approach allowed a fine study of defects which led to a model for defect creation in SWNT grown by CVD. A systematic study of RBM showed the processes leading to the preferential growth of small- or medium-diameter SWNT. Thus, control of catalyst thickness, of carbonaceous precursor nature and partial pressure or of synthesis temperature allows to control the diameter distribution of SWNT.

Xylella fastidiosa é uma bactéria limitada ao xilema de plantas, sendo transmitida principalmente por cigarrinhas da subfamília Cicadellinae (Hemiptera: Cicadellidae). No Brasil, é o agente causal da Clorose variegada dos citros (CVC), doença que afeta laranja-doce [Citrus sinensis (L.) Osbeck]. O objetivo deste trabalho foi caracterizar o comportamento alimentar da cicadelíneo vetor Bucephalogonia xanthophis (Berg) em citros e correlacionar suas atividades estiletares com a transmissão de X. fastidiosa. Inicialmente, testes de escolha e análises de excreção de honeydew foram realizadas para determinar os locais e períodos preferidos para alimentação em mudas de citros. B. xanthophis preferiu a haste dos ramos novos, na parte superior da muda. Esta cigarrinha ingeriu seiva do xilema e apresentou maior volume médio de excreção e maior percentual de indivíduos que excretaram durante a fotofase. Assim, estudou-se a penetração estiletar do vetor na haste de brotações cítricas, durante a fotofase, pela técnica de \"Electrical Penetration Graph\" (EPG, sistema DC). Os principais padrões de EPG foram correlacionados com análises histológicas e de \"honeydew\" para determinação da posição exata dos estiletes no tecido vegetal e atividades envolvidas. Seis padrões foram descritos: (S) secreção de bainha salivar e caminhamento dos estiletes através de células da epiderme ou parênquima; (R) estiletes inseridos na planta, porém sem nenhuma atividade aparente; (X) contato dos estiletes com os vasos do xilema; (Xi) ingestão ativa no xilema; (I) breve interrupção durante X ou Xi; (W) retirada dos estiletes da planta. Durante uma prova, a seqüência de eventos com maior probabilidade de ocorrência foi penetração estiletar através da epiderme e parênquima (S) (100% dos insetos), seguida de contato com o xilema (X) (67,6%). Entre os indivíduos que exibem o padrão X, 88,3% passam para Xi. Os vasos do xilema foram localizados pelo inseto após uma média de 2,2 provas. O tempo médio para atingir o xilema (X) e iniciar ingestão (Xi) após o início da primeira prova foi 27.8 min e 34,2 min, respectivamente. Entretanto, verificou-se ingestão prolongada no xilema (Xi > 5 min) somente após 39,8 min, em média. Em um outro estudo, investigou-se a relação dos padrões S, X e Xi com os processos de aquisição e inoculação de X. fastidiosa em citros. B. xanthophis adquiriu X. fastidiosa somente no padrão Xi. Após 1 h de ingestão no xilema, esta cigarrinha transmitiu a bactéria para plantas-teste com uma eficiência de 7,7%. A inoculação ocorreu durante os padrões S, S+X e S+X+Xi, com eficiência de 3,5; 7,1 e 7,4%, respectivamente. Um fato intrigante foi a ocorrência de inoculação de X. fastidiosa pelo inseto antes de atingir os vasos do xilema, durante o padrão S. Contudo, as maiores taxas de transmissão ocorreram após o contato com o xilema (S+X ou S+X+Xi). Por fim, estudou-se o efeito da infecção sintomática e assintomática por X. fastidiosa em plantas cítricas sobre a penetração estiletar de B. xanthophis. O comportamento alimentar foi semelhante em plantas sadias e infectadas sem sintomas. A infecção sintomática não afetou a capacidade de B. xanthophis localizar os vasos do xilema, mas reduziu o tempo gasto por este inseto ingerindo seiva dos mesmos. Estes resultados sugerem que a aquisição de X. fastidiosa pode ser mais eficiente em plantas infectadas assintomáticas do que em plantas com sintomas severos de CVC. As informações sobre penetração estiletar do vetor B. xanthophis em citros são importantes para estudos mais avançados de mecanismos de transmissão de X. fastidiosa, bem como para estabelecer estratégias que visem interferir neste processo. / Xylella fastidiosa is a xylem-limited bacterium transmitted mainly by leafhoppers (Hemiptera: Cicadellidae) in the subfamily Cicadellinae. In Brazil, it is the causal agent of Citrus variegated chlorosis, a disease that affects sweet orange [Citrus sinensis (L.) Osbeck]. The goal of this research was to characterize the feeding behavior of the cicadeline vector Bucephalogonia xanthophis (Berg) in citrus and correlate its feeding activities with transmission of X. fastidiosa. Initially, choice tests and honeydew excretion analyses were carried out to determine preferred feeding sites and periods on citrus nursery trees. B. xanthophis preferred the stems of young shoots, in the upper part of the plant. This species ingested sap from the xylem vessels and showed larger excretion volume and higher proportion of excreting individuals during the day. Thus, vector stylet penetration was studied on the stem of citrus shoots in the photophase, by using the Electrical Penetration Graph (EPG, DC system) technique. The main EPG waveforms were correlated with histological and honeydew excretion analyses to determine the precise stylet position in the plant tissue and feeding activities. Six waveforms and proposed activities are described: (S) secretion of salivary sheath and intracellular pathway; (R) stylets inserted into the plant, without any apparent activity; (X) contact of stylets with xylem vessels; (Xi) active xylem ingestion; (I) interruption between X and Xi; and (W) stylet withdrawal from the plant. During a probe, the most likely sequence of events is stylet pathway (S) through epidermal and parenchymal cells (all individuals), followed by contact with xylem (X) (67.6% of all individuals) and then active ingestion (Xi) (88.3% of those that exhibit waveform X). The xylem was reached after an average of 2.2 probes. The mean time to contact the xylem (X) and initiate ingestion (Xi) after onset of the first probe was 27.8 and 34.2 min, respectively. However, sustained xylem ingestion (Xi > 5 min) was established only after 39.8 min, on average. In a second study, the waveforms S, X and Xi were correlated with X. fastidiosa acquisition and inoculation in citrus. Acquisition of X. fastidiosa from infected plants occurred only after onset of the pathogen to 7.7% of test plants. In healthy plants, inoculation took place during waveform Xi (xylem ingestion); but with just 1 h in Xi, B. xanthophis subsequently transmitted waveforms S (salivary sheath formation and stylet pathway), S+X (X= first xylem contact by stylets) and S+X+X1, with efficiencies of 3.5, 7.1 and 7.4%, respectively. Although higher transmission rates were recorded after the first contact with xylem (S+X and S+X+Xi), it is intriguing the fact that inoculation of this xylem-limited bacterium also occurred before that (during S). Finally, the effect of X. fastidiosa infection on the feeding behavior of B. xanthophis was studied by comparing stylet penetration on: a) healthy citrus; b) symptomless infected citrus; c) infected citrus with CVC symptoms. Based on the analysis of 26 EPG parameters, no significant differences were found in stylet penetration on healthy versus asymptomatic infected citrus. Symptomatic infection did not affect the ability of B. xanthophis to locate xylem vessels, but reduced the time spend by this vector ingesting xylem sap. These results suggest that X. fastidiosa acquisition may be more efficient on symptomless infected plants than on citrus with severe CVC symptoms. Information on vector feeding behavior is basic for future studies on transmission mechanisms of X. fastidiosa and to establish control strategies aimed to interfere with this process.

Bakgrund: Patienter med hematologisk sjukdom erhöll en tunnellerad central venkateter i lokal anestesi inför behandling. Det är vanligt att patienter som är vakna under operation upplever oro. Syfte: Syftet med studien var att undersöka hur patienter med hematologisk sjukdom upplever att få en tunnelerad CVK i lokalanestesi samt hur de anser att upplevelsen kan förbättras. Metod: Studien har en deskriptiv design med kvalitativ metod. Semistrukturerade intervjuer genomfördes med 10 patienter där en intervjuguide med öppna frågor användes. Intervjuerna analyserades med hjälp av systematisk textkondensering enligt Malterud. Resultat: Gemensamt för samtliga patienter i studien var att CVK-ingreppet överlag var en positiv upplevelse. Patienterna beskrev att kontinuerlig information och ett gott omhändertagande på operation bidrog till en positiv upplevelse av att få en tunnelerad CVK. I resultatet framkom fyra teman; 1- mental styrka hjälpte patienterna att känna sig redo, 2- att vara utsatt i en miljö där smärta och obehag förekom, 3- omhändertaget av professionell och erfaren personal bidrog till en lugn stämning, 4- tidigare erfarenheter och information minskade oro och gav patienterna ökad trygghet. Slutsats: Studien visar att det finns potential att göra upplevelsen av att få en tunnelerad CVK bättre med enkla medel. Förbättringen kan ske genom tydligare information, varmare temperatur inne på operationssalen och genom att anestesisjuksköterskan stödjer patienten vid oro. Informationen och vården behöver således bli mer personcentrerad och vårdpersonal behöver öva för att bli bättre på att ge denna vård. / Background: Prior to treatment, patients with haematological disease receives a tunnelled central venous catheter in local anesthesia. It is common for patients who are awake during surgery to experience anxiety. Aim: The aim of this study was to describe how patients suffering from hematological diseases experience tunelled central venous cathetrization in local anesthesia and how their experience could be improved. Design: Semistructured interviews using an interview guide with open-ended questions where performed with 10 patients. Data were analysed with systematic text condensation according to Malterud. Result: The included patients had an overall positive experience from recieving a tunelled central venous catheter. The patients described how continuous information and good care improved their experience of recieving a tunelled central venous catheter. Four themes were identified in the result; 1- having mental a strenght made the patients feel relieved and prepared, 2- being exposed to an environment where pain and discomfort were present, 3- being taken care of by experienced professionals contributed to a calm atmosphere, 4- previous experiences and information reduced anxiety and promoted an increased feeling of safety Conclusion: The present study shows the potential to make patients experiences better by simple means, for example by giving more distinct information, raising the temperature in the operating room and by the nurse anesthetist acting more supportive when patients feel anxiety. The information and nursing care need to be more personalized and professionals need to practice in order to improve.

Precision measurements of superallowed Fermi β-decay allow for stringent tests of the magnitude of isospin-symmetry-breaking effects in nuclei, the validity of the conserved vector current hypothesis, and the unitarity of the Cabibbo-Kobayashi-Maskawa (CKM) quark-mixing matrix. A high-precision measurement of the branching ratio for the superallowed β+ decay of 74Rb has been performed at the Isotope Separator and ACcelerator (ISAC) facility at TRIUMF. The 8π spectrometer, an array of 20 HPGe detectors, was used to detect γ-rays emitted following the Gamow-Teller and non-analogue Fermi β-decays of 74Rb. PACES, an array of 5 Si(Li) detectors, was used to detect emitted conversion electrons, while half of SCEPTAR, a close-packed array of 10 plastic scintillators, was used to detect emitted β particles. In this experiment, 23 excited states were identified in 74Kr which were populated following the β-decay of 74Rb. A total of 58 γ-rays were identified following the 8.241(4)x108 detected β-particles. An observed non-superallowed branching ratio of 0.396(7)% was determined from the intensity of the identified γ-rays, while the unobserved non-superallowed intensity was calculated to be 0.05(5)%, leading to a superallowed branching ratio of 99.55(5)%. The superallowed branching ratio is now the most precise experimental quantity in the determination of the superallowed ft-value of 74Rb. Combining the half-life and Q-value with the superallowed branching ratio measured in this work leads to a superallowed ft-value of 3082.6(66) s. Finally, comparisons between the superallowed ft-value, the world average Ft-value, and the non-analogue Fermi branching ratio are made to provide future guidance in the refinement of the theoretical models required to describe the crucial isospin-symmetry-breaking term in superallowed β-decay.

Bakgrund: Vårdrelaterade infektioner är ett stort problem inom dagens sjukvård och en av de vanligaste vårdrelaterade infektionerna inom intensivvården är kateterrelaterade. En central venkateter (CVK) är en kärlaccess som är vanligt förekommande inom intensivvården och handhavandet av dessa utförs till största del av sjuksköterskan. Sjuksköterskans förebyggande arbete är därför viktigt för att minska förekomsten av CVK-relaterade infektioner samt öka patientsäkerheten. Syfte: Syftet med litteraturstudien var att beskriva vilka CVK-relaterade åtgärder utförda av sjuksköterskor hos vuxna patienter inom intensivvård som minskar risken för vårdrelaterade infektioner. Metod: En strukturerad litteraturstudie som sammanställt kvantitativ forskning med en induktiv innehållsanalys. Antal studier som inkluderades i resultatet var 18 stycken. Huvudresultat: Litteraturstudiens huvudresultat baserades på fem teman, samtliga beskrev preventiva omvårdnadsåtgärder. Resultatet inom respektive tema var vikten av mekanisk rengöring med desinfektionsmedel av injektionsportar och trevägskranar, daglig utvärdering av patientens behov av en CVK, en klorhexidinkomponent i förbandet, daglig helkroppsavtvättning med tvättlappar innehållande klorhexidin samt utbildning av personalen kring evidensbaserade riktlinjer. Samtliga dessa områden sågs minska risken och förekomsten av CVK-relaterade infektioner. Slutsats: Det är angeläget att det finns sjukhusövergripande riktlinjer som baseras på den bästa tillgängliga evidensen i syfte att minska CVK-relaterade infektioner inom intensivvården. Dessa bör vara samstämmiga med de nationellt övergripande riktlinjerna. Detta för att skapa en så jämlik och patientsäker vård som möjligt. / Introduction: Healthcare-related infections are a major problem in today's healthcare and one of the most common care related infections in the intensive care unit is catheter related. A central venous catheter (CVC) is a vascular access commonly found in intensive care units, and the maintenance is commonly performed by the nurses. The preventive work is therefore important in reducing the incidence of CVC-related infections and by that increasing the patient safety.   Aim: The aim of the study was to describe what part of maintenance regarding the CVC performed by nurses in adult patients in the intensive care unit that reduces the risk of healthcare-related infections. Method: A structured literature study was conducted and studies with a quantitative design were compiled. The results were analyzed with an inductive content analysis. The number of articles included in the results were 18. Main results: The main results of the study were based on five themes which were preventive measures. The results were mechanical cleaning with disinfectants of needleless connectors and three-way stop cork, daily evaluation of the patients need for a CVC, chlorhexidine dressing, daily chlorhexidine baths and education of staff. These areas were seen to reduce the risk and incidence of CVC-related infections. Conclusion: It is important that there is a consensus of the guidelines regarding the preventions of CVC-related infections both locally and nationally. The guidelines should be based on the best available evidence to reduce CVC-related infections in the intensive care unit. The purpose is to perform equal care and to promote patient safety.

Xylella fastidiosa é uma bactéria limitada ao xilema de plantas, sendo transmitida principalmente por cigarrinhas da subfamília Cicadellinae (Hemiptera: Cicadellidae). No Brasil, é o agente causal da Clorose variegada dos citros (CVC), doença que afeta laranja-doce [Citrus sinensis (L.) Osbeck]. O objetivo deste trabalho foi caracterizar o comportamento alimentar da cicadelíneo vetor Bucephalogonia xanthophis (Berg) em citros e correlacionar suas atividades estiletares com a transmissão de X. fastidiosa. Inicialmente, testes de escolha e análises de excreção de honeydew foram realizadas para determinar os locais e períodos preferidos para alimentação em mudas de citros. B. xanthophis preferiu a haste dos ramos novos, na parte superior da muda. Esta cigarrinha ingeriu seiva do xilema e apresentou maior volume médio de excreção e maior percentual de indivíduos que excretaram durante a fotofase. Assim, estudou-se a penetração estiletar do vetor na haste de brotações cítricas, durante a fotofase, pela técnica de \"Electrical Penetration Graph\" (EPG, sistema DC). Os principais padrões de EPG foram correlacionados com análises histológicas e de \"honeydew\" para determinação da posição exata dos estiletes no tecido vegetal e atividades envolvidas. Seis padrões foram descritos: (S) secreção de bainha salivar e caminhamento dos estiletes através de células da epiderme ou parênquima; (R) estiletes inseridos na planta, porém sem nenhuma atividade aparente; (X) contato dos estiletes com os vasos do xilema; (Xi) ingestão ativa no xilema; (I) breve interrupção durante X ou Xi; (W) retirada dos estiletes da planta. Durante uma prova, a seqüência de eventos com maior probabilidade de ocorrência foi penetração estiletar através da epiderme e parênquima (S) (100% dos insetos), seguida de contato com o xilema (X) (67,6%). Entre os indivíduos que exibem o padrão X, 88,3% passam para Xi. Os vasos do xilema foram localizados pelo inseto após uma média de 2,2 provas. O tempo médio para atingir o xilema (X) e iniciar ingestão (Xi) após o início da primeira prova foi 27.8 min e 34,2 min, respectivamente. Entretanto, verificou-se ingestão prolongada no xilema (Xi > 5 min) somente após 39,8 min, em média. Em um outro estudo, investigou-se a relação dos padrões S, X e Xi com os processos de aquisição e inoculação de X. fastidiosa em citros. B. xanthophis adquiriu X. fastidiosa somente no padrão Xi. Após 1 h de ingestão no xilema, esta cigarrinha transmitiu a bactéria para plantas-teste com uma eficiência de 7,7%. A inoculação ocorreu durante os padrões S, S+X e S+X+Xi, com eficiência de 3,5; 7,1 e 7,4%, respectivamente. Um fato intrigante foi a ocorrência de inoculação de X. fastidiosa pelo inseto antes de atingir os vasos do xilema, durante o padrão S. Contudo, as maiores taxas de transmissão ocorreram após o contato com o xilema (S+X ou S+X+Xi). Por fim, estudou-se o efeito da infecção sintomática e assintomática por X. fastidiosa em plantas cítricas sobre a penetração estiletar de B. xanthophis. O comportamento alimentar foi semelhante em plantas sadias e infectadas sem sintomas. A infecção sintomática não afetou a capacidade de B. xanthophis localizar os vasos do xilema, mas reduziu o tempo gasto por este inseto ingerindo seiva dos mesmos. Estes resultados sugerem que a aquisição de X. fastidiosa pode ser mais eficiente em plantas infectadas assintomáticas do que em plantas com sintomas severos de CVC. As informações sobre penetração estiletar do vetor B. xanthophis em citros são importantes para estudos mais avançados de mecanismos de transmissão de X. fastidiosa, bem como para estabelecer estratégias que visem interferir neste processo. / Xylella fastidiosa is a xylem-limited bacterium transmitted mainly by leafhoppers (Hemiptera: Cicadellidae) in the subfamily Cicadellinae. In Brazil, it is the causal agent of Citrus variegated chlorosis, a disease that affects sweet orange [Citrus sinensis (L.) Osbeck]. The goal of this research was to characterize the feeding behavior of the cicadeline vector Bucephalogonia xanthophis (Berg) in citrus and correlate its feeding activities with transmission of X. fastidiosa. Initially, choice tests and honeydew excretion analyses were carried out to determine preferred feeding sites and periods on citrus nursery trees. B. xanthophis preferred the stems of young shoots, in the upper part of the plant. This species ingested sap from the xylem vessels and showed larger excretion volume and higher proportion of excreting individuals during the day. Thus, vector stylet penetration was studied on the stem of citrus shoots in the photophase, by using the Electrical Penetration Graph (EPG, DC system) technique. The main EPG waveforms were correlated with histological and honeydew excretion analyses to determine the precise stylet position in the plant tissue and feeding activities. Six waveforms and proposed activities are described: (S) secretion of salivary sheath and intracellular pathway; (R) stylets inserted into the plant, without any apparent activity; (X) contact of stylets with xylem vessels; (Xi) active xylem ingestion; (I) interruption between X and Xi; and (W) stylet withdrawal from the plant. During a probe, the most likely sequence of events is stylet pathway (S) through epidermal and parenchymal cells (all individuals), followed by contact with xylem (X) (67.6% of all individuals) and then active ingestion (Xi) (88.3% of those that exhibit waveform X). The xylem was reached after an average of 2.2 probes. The mean time to contact the xylem (X) and initiate ingestion (Xi) after onset of the first probe was 27.8 and 34.2 min, respectively. However, sustained xylem ingestion (Xi > 5 min) was established only after 39.8 min, on average. In a second study, the waveforms S, X and Xi were correlated with X. fastidiosa acquisition and inoculation in citrus. Acquisition of X. fastidiosa from infected plants occurred only after onset of the pathogen to 7.7% of test plants. In healthy plants, inoculation took place during waveform Xi (xylem ingestion); but with just 1 h in Xi, B. xanthophis subsequently transmitted waveforms S (salivary sheath formation and stylet pathway), S+X (X= first xylem contact by stylets) and S+X+X1, with efficiencies of 3.5, 7.1 and 7.4%, respectively. Although higher transmission rates were recorded after the first contact with xylem (S+X and S+X+Xi), it is intriguing the fact that inoculation of this xylem-limited bacterium also occurred before that (during S). Finally, the effect of X. fastidiosa infection on the feeding behavior of B. xanthophis was studied by comparing stylet penetration on: a) healthy citrus; b) symptomless infected citrus; c) infected citrus with CVC symptoms. Based on the analysis of 26 EPG parameters, no significant differences were found in stylet penetration on healthy versus asymptomatic infected citrus. Symptomatic infection did not affect the ability of B. xanthophis to locate xylem vessels, but reduced the time spend by this vector ingesting xylem sap. These results suggest that X. fastidiosa acquisition may be more efficient on symptomless infected plants than on citrus with severe CVC symptoms. Information on vector feeding behavior is basic for future studies on transmission mechanisms of X. fastidiosa and to establish control strategies aimed to interfere with this process.

A single-subject-multiple-baseline-across-behaviors design was used to examine the effects of using Systematic and Engaging Early Literacy (SEEL) instruction to help Tier 3 kindergarten students learn to read CVC words. Four students designated as Tier 3 by their teachers participated in the study. They were grouped into two dyads and received SEEL instruction focusing on specific word reading targets for approximately 20 minutes four days per week over a seven-week time period. The instruction included meaningful, interactive activities and incorporated high levels of play, multiple exposures to the target, explicit instruction, and student-teacher conversational exchanges. Baseline assessment data were collected prior to the application of the intervention for each of the targets and assessment data continued to be collected after each intervention session. All students learned to read the target words and three of the students generalized their learning to other targets. A moderate to large effect size of 0.54 was obtained using Cohen's r value. The need for adequate exposure to targets and time to practice was highlighted, along with the value of revisiting targets and addressing individual student's needs when working in small groups.

Bakgrund: CVK-relaterade infektioner i blodbanan är förenat med förlängd sjukhusvistelse, ökad mortalitet, ökade ekonomiska kostnader samt ökat lidande för patienten. Användningen av centrala venösa katetrar ökar och därmed är risken att drabbas av infektion större. Det finns en bristande kunskap hos sjuksköterskan gällande förebyggandet av dessa infektioner och en bristande följsamhet till riktlinjer. Syfte: Beskriva hur sjuksköterskan kan förebygga CVK-relaterade infektioner i blodbanan. Metod: Allmän litteraturstudie av kvantitativa artiklar. Resultat: En kombination av utbildning, praktiska åtgärder såsom skötsel, hygienrutiner och checklistor samt feedback minskade antalet CVK-relaterade infektioner i blodbanan. Slutsatser: Utbildning, olika typer av praktiska åtgärder samt feedback minskar antalet CVK-relaterade infektioner i blodbanan och är viktigt för sjuksköterskans förebyggande vård. En stor del av vad som framkom i resultat stämmer överens med riktlinjerna för förebyggandet av CVK-relaterade infektioner, dock bör utbildning och feedback prioriteras i högre utsträckning. Det är känt sedan tidigare att följsamhet till riktlinjer bland sjuksköterskor skulle underlätta utförandet av evidensbaserad omvårdnad. Mer fokus bör därför ligga på att få sjuksköterskor att följa riktlinjer. Klinisk betydelse: Studien kan bidra med ökad kunskap hos sjuksköterskor om hur CVK-relaterade infektioner i blodbanan kan förebyggas vilket i sin tur skulle kunna generera bättre vård, minskat lidande för patienten, minskad mortalitet samt ekonomiska besparingar. / Background: CVC-related bloodstream infections often lead to prolonged hospitalization, increased mortality, higher economical cost and more suffering for the patient. The usage of central venous catheters increases and therefore the risk of infection is also higher. When it comes to prevention of these infections there is a lack of knowledge amongst nurses and compliance with the guidelines. Aim: Describe how the nurse can prevent CVC-related bloodstream infections. Method: General literature review of quantitative studies. Results: A combination of education, hands-on initiatives such as management, hygieneroutines and checklists as well as feedback turned out to reduce CVC-related bloodstream infections. Conclusions: Education, different hands-on initiatives and feedback decreases the number of CVC-related bloodstream infection and is essential for the nurse’s preventive care. Most of the results correlate with the guidelines for the prevention of CVC-related bloodsteam infections, nevertheless education and feedback should be prioritized in higher extent. It is known that adherence to guidelines among nurses would facilitate the execution of evidence-based nursing. Therefore more focus should be on having nurses follow the guidelines. Clinical importance: This study would contribute with increased knowledge amongst nurses regarding prevention of CVC-related bloodstream infections which would give better care, diminish the suffering of the patient, decrease mortality and lead to economical benefits.