Cochliomyia macellaria (Fabricius) has great importance in forensics and is commonly utilized in estimating the period of insect activity (PIA) on a corpse due to its rapid colonization time of fresh remains, active oviposition during daylight, and its abundance throughout the southern United States. The purpose of this study is to determine what effects tissue and temperature will have on C. macellaria. The purpose of my secondary studies on attraction to larval resource is to gain insight into the behavior of adult blow flies in order to determine what factors drive colonization of remains. Forensic entomology can use development data of calliphorid flies to estimate their PIA on human remains. C. macellaria has major implications in medico-legal entomology as well as in veterinary entomology and are often found on human remains in Texas during the warm months of the year. Furthermore, C.macellaria is a secondary myiasis producer; this means that its larvae may be found infesting living animal or human muscle tissue and potentially could be involved in veterinary forensic cases. This study will help provide better insight on the behavior of C. macellaria in Texas as it relates to tissue type and temperature. In this experiment larvae were reared at three separate temperatures (21°C, 24°C, and 27°C) on either equine or porcine striated muscle tissues. Eggs were inoculated onto each tissue and monitored every hour for hatch. Once hatch occurred, observations were shifted to every twelve hours. Three larvae were sampled during each observation period. Sampled larvae were weighed and length was recorded. Additionally, stage of development was determined. Pupae were collected and time to adult emergence recorded. Furthermore, life-history traits, such as emergence pattern and adult longevity, were recorded. This study is the first in Texas and second in the United States to examine the development of C. macellaria and could provide significant information for cases of myiasis and neglect of both humans and animals. In contrast, rearing flies on beef liver or pork chops, which tends to be a standard procedure often employed may not be applicable to myiasis cases of equines. Data from this study could provide greater insight to developmental differences of forensically important blow flies on striated muscle from different vertebrate species. Since porcine tissue was used in the Florida study, it was also utilized in our study in order to allow a comparison. To date there is only one data set on the development of C. macellaria in the United States. Fly populations in different climates are suspected to have different development rates. This study will compare data from central Texas to data generated in Florida. If variation of development rates is demonstrated for different populations, then forensic entomologists should use data that are more conducive to their given geographic area. My secondary objective first looked at whether larval resource had an attraction effect on the subsequent adult C. macellaria flies. One cohort of larvae was reared on bovine testicles, while another was reared on bovine liver. The subsequent adults were then tested in a dual-choice olfactometer which possessed testicles at one end and liver at the other end. The second set of experiments also involved using a dual-choice olfactometer; however, all larvae were reared on bovine liver and fresh liver was placed at one end of the olfactometer, while fresh liver with conspecific larvae was placed at the end of the other. The purpose of this experiment was to assess whether adult C. macellaria flies were more attracted to the presence of conspecific larvae. These experiments will give us more insignt into adult blow fly behavior and help us to understand what factors drive colonization of remains.

Made available in DSpace on 2014-06-11T19:23:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-09-24Bitstream added on 2014-06-13T19:49:30Z : No. of bitstreams: 1 lima_cgp_me_botib.pdf: 1317416 bytes, checksum: 0a405074b77c54d73fd403b54efb898d (MD5) / A Entomologia Forense utiliza dados de desenvolvimento e aspectos ecológicos de insetos que se alimentam de corpos em decomposição com o objetivo de auxiliar a resolução de casos na área forense. Uma das principais aplicações, entre outras, é a estimativa do intervalo pós-morte (IPM) que pode ser baseada no ciclo biológico de uma dada espécie. Porém, a falta de informação ou conhecimento sobre as variáveis que interferem de forma direta ou indireta sobre a taxa de desenvolvimento de diferentes espécies pode gerar dados imprecisos em relação à idade do inseto e, consequentemente, ao IPM. Recentemente, devido ao aumento no número de óbitos relacionados ao abuso de drogas, análises toxicológicas têm sido direcionadas a insetos necrófagos, visando complementar informações acerca da causa da morte, assim como garantir maior acurácia ao cálculo do IPM, uma vez que certas substâncias ingeridas antes do óbito podem alterar a taxa de desenvolvimento de algumas espécies que se alimentam dos tecidos de cadáveres. Esse ramo mais recente da entomologia forense é conhecido como Entomotoxicologia. No presente estudo objetivou-se observar o efeito de diferentes concentrações de metanfetamina e ecstasy, derivados anfetamínicos que tiveram seu consumo aumentado nas últimas décadas no Brasil, na taxa de desenvolvimento de imaturos dos califorídeos Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius) e Chrysomya putoria (Wiedemann), quando acrescidos em dieta artificial apropriada. A anfetamina, metabólito da metanfetamina, não afetou de forma significativa o desenvolvimento larval de C. megacephala, no entanto, aumentou o tempo de desenvolvimento de imaturos de C. putoria, em especial a fase de pupa. O MDMA, principal componente do ecstasy, não alterou a taxa de desenvolvimento larval de C. albiceps e C. putoria, mas acelerou consideravelmente... / Forensic Entomology uses data about development and ecology of insects that feed on corpses aiming to assist to solve forensic cases. One of its main aplication, among others, is to estimate the pos-mortem interval (PMI) that can be based on the life cycle of a specific species. However, the lack of information or knowledge about the variables that can change directly or indirectly the development rate of different species can lead to inaccurate data about the age of the insect and, consequently, the PMI. Recently, due to the increasing number of death related to drug abuse, toxicological analyses have been directed to necrophagous insects intending to provide complementary information about the cause of death, as well as to asure a more acurate estimate of PMI, once some substances ingested before death can modify the development rate of some species that feed on cadaver tissues. This new branch of Forensic Entomology is known as Entomotoxicology. The aim of the present study was to observe the effect of different concentrations of methamphetamine and ecstasy, two amphetamine-like drugs which abuse rose among Brazilians in the last decades, on the development rate of immature of Calliphoridae Chrysomya albiceps (Wiedemann), Chrysomya megacephala (Fabricius) and Chrysomya putoria (Wiedemann), when added to artificial diet proper for them to complete their development. Amphetamine, methamphetamine´s metabolite, did not affect significantly the larval development of C. megacephala, although it increased the time of development of C. putoria maggots, specially the pupa stage. MDMA, the main compound of ecstasy, did not alter the larval development rate of C. albiceps nor C. putoria but it accelerated, considerably, the development rate of C. megacephala. Through immunohistochemical analyses it was tried to detect amphetamine in larvae of C. megacephala and C. putoria, but the results were... (Complete abstract click electronic access below)

Made available in DSpace on 2014-08-20T14:31:32Z (GMT). No. of bitstreams: 1 dissertacao_sabrina_pires_capa.pdf: 403567 bytes, checksum: 5752dd89517a54f40cd0632e1db1c1c6 (MD5) Previous issue date: 2007-03-30 / The blowflies are the first insects to compose the faunistic succession of human cadavers, thus helping to determine the postmortem interval (PMI). However, failing to consider larval post-feeding dispersal can compromise medical-criminal inquiries. In order to investigate the relationship between post- feeding Lucilia sericata larvae and their weight, radial dispersion and depth, an artificial arena was built simulating the natural environment. We observed a difference in mean pupal weight between males (30.28mg) and females (32.35mg). We also observed that the majority of pupae were recovered a distance of 33 to 45cm from the center of the arena, and at a depth of 6 to 8cm from the surface. However, we observed no differences in radial dispersion and depth of pupation between males, females or unviable individuals. Correlation analysis between weight and distance (r = 0.246) and between weight and depth (r = 0.321) was directly proportional, suggesting that heavier pupae dispersed farther and deeper than lighter pupae. The experiment also allowed us to conclude that a circular arena permitted larval dispersion all directions. / Os dípteros califorídeos são os primeiros indivíduos a compor a sucessão faunística dos cadáveres humanos auxiliando assim na estimativa do intervalo pós- morte. Porém, a não consideração da dispersão pós-alimentar de larvas pode comprometer as investigações médico-criminais. Diante disto uma arena circular, simulando o ambiente natural, foi montada em laboratório a fim de verificar-se a dispersão larval radial pós-alimentar de Lucilia sericata, tentando assim estabelecer relações entre as variáveis peso, distância e profundidade de enterramento na arena. Os resultados demonstraram que a maioria das pupas foram recuperadas a uma profundidade compreendida entre 6cm e 8cm e a uma distância entre 33cm e 45cm do centro da arena. Não houve diferenças significativas quanto a propagação e profundidade de enterramento de machos, fêmeas e indivíduos inviáveis. Com relação ao peso, verificou-se que a média das fêmeas (x = 32,35mg) foi superior a dos machos (x = 30,28mg). A análise de correlação entre peso e distância percorrida (r = 0,246) e entre peso e profundidade (r = 0,321) foi diretamente proporcional, ou seja pupas mais pesadas propagaram e se aprofundaram mais. O experimento permitiu ainda concluir que uma arena circular possibilita o deslocamento das larvas em todas as direções.

Made available in DSpace on 2014-08-20T14:37:52Z (GMT). No. of bitstreams: 1 dissertacao_ciciane_fernandes.pdf: 1291718 bytes, checksum: 7919ec746c53767068a313c9fd4b16f2 (MD5) Previous issue date: 2013-02-08 / The aim was to evaluate the healing of open cutaneous wounds of Wistar rats treated with Carapa guianensis (andiroba) and Caesalpinia ferrea Mart. (Jucá) and their repellent action against flies of the Calliphoridae family. It were studied 160 Wistar rats with open wounds in the back that were treated daily with Carapa guianensis at concentrations of 20% (A20) and 50% (A50), Caesalpinia ferrea Mart. concentrations at 20% (J20) and 50% (J50) and Vaseline (control group). Clinical, histological and morphometric studies were carried out after four, seven, 14 and 21 days of treatment, as well as a tensiometric study after 21 days. In order to study the repellency, W.O.T traps (Wind Oriented Trap) containing deteriorated bovine liver and cream with the herbal medicine Carapa guianensis and Caesalpinia ferrea Mart. at concentrations of 20% and 50% were used to catch flies. Clinically, after four days of treatment, the wounds of the control group presented exudate, differing statistically from the other groups (p=0.0065). In the morphometric study, J50 and J20 groups had higher average area (p=0.0001) after seven days of treatment, whereas after 14 days, the wounds of the control group had higher average area (p=0.0000) compared with the other groups. In the histopathological study, the differences between groups were seen after four days of treatment, with the A20 group showing a greater number of wounds in the proliferative phase (p=0.0000). In the tensiometric study, the control group showed better results (3.52MPa) in tension when compared to other groups (p=0.0055). In the repellency study, the traps containing J50 (p<= 0.010) and D20 (p<= 0.010) showed a higher repellency 97% and 100%, respectively, followed by the A50 group (p<= 0.010) with 93.4% and the A20 group with 56.5%. The results led to the following conclusions: Carapa guianensis at a concentration of 20% accelerates the phases of the healing process in the first days after tissue injury, whereas Carapa guianensis at 50% and Caesalpinia ferrea Mart. at concentrations of 20% and 50% do not show satisfactory results as a cicatrizant. As repellent action, Caesalpinia ferrea Mart. at concentrations of 50% and 20% and Carapa guianensis at 50% have repellent effect against Chrysomya albiceps, Chrysomya megacephala, Lucilia cuprina, Lucilia eximia, Lucilia sericata and Sarconesia chlorogaster flies, species of the Calliphoridae family, while the Carapa guianensis at 20% has less repellent action. / Objetivou-se avaliar a cicatrização de feridas cutâneas abertas de ratos Wistar tratadas com Carapa guianensis (andiroba) e Caesalpinia ferrea Mart. (jucá) e, suas ações repelentes frente a moscas da família Calliphoridae. Foram estudados 160 ratos da linhagem Wistar, com realização de feridas cutâneas abertas no dorso, tratadas diariamente com Carapa guianensis nas concentrações de 20% (A20) e 50% (A50), Caesalpinia ferrea Mart. nas concentrações de 20% (J20) e 50% (J50) e vaselina (grupo controle), sendo realizadas avaliações clínicas, morfométricas e histológicas aos quatro, sete , 14 e 21 dias de tratamento e estudo tensiométrico aos 21 dias. Para estudo da repelência, foram utilizadas armadilhas W.O.T. (Wind Oriented Trap) para captura de moscas contendo fígado bovino deteriorado e creme contendo os fitoterápicos Carapa guianensis e Caesalpinia ferrea Mart. nas concentrações de 20% e 50%. Clinicamente, aos quatro dias de tratamento, as feridas tratadas apresentaram formação de crosta, diferindo estatisticamente do grupo controle que ainda apresentava exsudato nas feridas (p=0,0065). No estudo morfométrico, aos sete dias de tratamento, os grupos J50 e J20 apresentaram maior média de área (p=0,0001), enquanto que aos 14 dias, as feridas do grupo controle apresentaram maior média de área (p=0,0000) comparada com os demais grupos. Na histopatologia foram observadas diferenças entre os grupos aos quatro dias de tratamento, com o grupo A20 apresentando maior número de feridas na fase proliferativa (p=0,0000). No estudo tensiométrico, o grupo controle apresentou melhor resultado (3.52MPa) na tensão comparado aos demais grupos (p=0,0055). No estudo sobre repelência, as armadilhas contendo J50 (p<= 0,010) e J20 (p<= 0,010) apresentaram maior repelência respectivamente 97 e 100%, seguida do grupo A50 (p<= 0,010) com 93,4% e grupo A20 com 56,5%. Os resultados levaram as seguintes conclusões: Carapa guianensis na concentração de 20% acelera o processo cicatricial nos primeiros dias após injúria tecidual, enquanto que Carapa guianensis a 50% e Caesalpinia ferrea Mart. nas concentrações de 20% e 50% não apresentam resultados satisfatórios como cicatrizante. Como ação repelente, Caesalpinia ferrea Mart. nas concentrações de 50% e 20% e Carapa guianensis na concentração de 50% apresentam efeito repelente frente às moscas Chrysomya albiceps, Chrysomya megacephala, Lucilia cuprina, Lucilia eximia, Lucilia sericata e Sarconesia chlorogaster, espécies da família Calliphoridae, enquanto que Carapa guianensis a 20% apresenta menor ação repelente.

Made available in DSpace on 2014-06-11T19:30:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-10-27Bitstream added on 2014-06-13T19:40:33Z : No. of bitstreams: 1 nitsche_mjt_dr_botib.pdf: 6040956 bytes, checksum: a5d0aa6950f59bac900d930691715424 (MD5) / A terapia larval consiste na aplicação de larvas vivas estéreis de dípteros obtidas em laboratório sobre lesões, feridas crônicas ou infectadas tendo como finalidade a cicatrização a partir da remoção de secreção e tecido necrosado pelo inseto facilitando, assim, o processo de cicatrização. Apesar da fácil aplicabilidade e do baixo custo, para garantir maior segurança e sucesso em relação a este procedimento dois fatores têm de ser alcançados: a esterilidade das larvas a serem aplicadas e a garantia de que a espécie selecionada para este fim fará uso, durante o seu processo de alimentação na ferida, somente de tecido necrosado. No presente estudo foi avaliada a esterilização e a viabilidade pós-esterilização de larvas de Chrysomya megacephala e Chrysomya putoria (Diptera: Calliphoridae) em hipoclorito de sódio a 0,5% e 1,0%. Adicionalmente, foi avaliado o desempenho (qualidade e tempo de tratamento) dos imaturos dessas mesmas espécies no desbridamento de feridas cutâneas induzidas, usando como modelo animal ratos Wistar. A escolha das espécies foi motivada pela facilidade de obtenção em todo território brasileiro, levando em conta ainda seu comportamento e biologia já descritos em literatura. Os melhores resultados em relação à sobrevida e viabilidade das larvas foram obtidos com o uso do hipoclorito de sódio a 0,5% como agente esterilizante. Após o processo de esterilização, 5-10 larvas/cm2 com 24 horas de vida pós-eclosão, foram aplicadas sobre as lesões induzidas, as quais por sua vez foram cobertas com gaze estéril e esparadrapo. Após 16 horas o curativo foi removido, constatando que as larvas ainda se encontravam vivas e permaneciam apenas no local da lesão, sem invadir tecidos saudáveis, havendo desaparecido o tecido necrótico e as secreções. No grupo controle, sem aplicação de larvas, o tecido necrosado e a secreção... / The larval therapy involves the application of sterile larvae of flies obtained in the laboratory on injuries, chronic wounds or infected with the purpose to heal from the removal of necrotic tissue and secretion by the insect, thus facilitating the cicatrization process. Despite the easy applicability and low cost, to ensure greater safety and success in relation to this procedure two factors must be achieved: the sterility of the larvae to be applied and ensure that the species selected for this purpose will use during their feeding process in the wound, only necrotic tissue. In this study we evaluated the sterilization and viability poststerilization of the larvae of Chrysomya megacephala and Chrysomya putoria (Diptera: Calliphoridae) in sodium hypochlorite 0.5% and 1.0%. Additionally, we evaluated the performance (quality and length of treatment) of immatures of these same species in the debridement of wounds induced, using rats as animal model. The choice of species was motivated by the ease of collecting around Brazil, taking into account their behavior and biology has already been described in literature. The best results regarding the viability and survival of larvae were obtained from the use of sodium hypochlorite at 0.5% as a sterilizing agent. After the sterilization process, 5-10 larvae/cm2 with 24 hours of post-hatching, were applied over the induced lesions, which it were covered with gauze and adhesive tape. After 16 hours the bandage was removed, noting that the larvae were still alive and remained only at the injury site, without invading healthy tissue, having gone necrotic tissue and secretions. In the control group without the application of maggots, the necrotic tissue and secretion increased in quantity, considering the same interval of treatment biotherapic. Skin fragments with a size of 1 cm2 from the margin to the center of the lesion were removed from ... (Complete abstract click electronic access below)

There have been no landscape-scale studies on earthworm populations in Canada comparing vegetation types; previous studies on edge habitats have been conducted in agricultural systems. I examined the spatial variations of earthworm populations by measuring abundance based on regional municipality, vegetation type, and edge habitat. Earthworms were sampled throughout the season across a gradient of vegetation types including meadow, forest edge, and interior at a local-scale; and at the landscape level with vegetation types including meadow, deciduous forest, pine plantation and mixed forest. Regional effects were more significant than vegetation type likely due to a gradient of soil characteristics in southern Ontario; edges had intermediate earthworm abundance and a higher proportion of epigeic species. My research provides insight into the patterns of earthworm populations in southern Ontario and the possible effects of edge creation through landscape fragmentation. Field sampling of earthworm parasitoid cluster-flies (Calliphoridae: Pollenia) using synomones was also discussed.

There have been no landscape-scale studies on earthworm populations in Canada comparing vegetation types; previous studies on edge habitats have been conducted in agricultural systems. I examined the spatial variations of earthworm populations by measuring abundance based on regional municipality, vegetation type, and edge habitat. Earthworms were sampled throughout the season across a gradient of vegetation types including meadow, forest edge, and interior at a local-scale; and at the landscape level with vegetation types including meadow, deciduous forest, pine plantation and mixed forest. Regional effects were more significant than vegetation type likely due to a gradient of soil characteristics in southern Ontario; edges had intermediate earthworm abundance and a higher proportion of epigeic species. My research provides insight into the patterns of earthworm populations in southern Ontario and the possible effects of edge creation through landscape fragmentation. Field sampling of earthworm parasitoid cluster-flies (Calliphoridae: Pollenia) using synomones was also discussed.

It is commonly thought that blow flies are nocturnally inactive. Blowflies are often important in helping to estimate post mortem intervals (PMI) for corpses found at death scenes. If blow flies oviposit during nocturnal hours, there could possibly be up to 12-hour discrepancies for estimates of PMI. Two blow fly species, Phaenicia eximia (Wiedemann) and Cochliomyia macellaria (F.), oviposited nocturnally on ground beef baits in 2003, during the onset of low-atmospheric pressure (an air mass with lower atmospheric pressure than that of the air surrounding it) at rural study sites near Snook (Burleson County), Texas, under artificial lighting of at least 1500 footcandles when temperatures were at 26?C or higher. Trials conducted under similar conditions during the same year without a low-pressure system moving into the area resulted in no nocturnal blow fly oviposition. Nocturnal blow fly oviposition went undetected once low-atmospheric pressure systems settled into the study area, irrespective of temperature and lighting conditions. Thus, this study illustrates that there is a connection between the onset of low-atmospheric pressure and nocturnal blow fly oviposition on baits under artificial lighting of 1500 footcandles or more when temperatures are 26 ?C or above. After concluding 2003 experiments, statistical analyses supplied evidence that woodland habitat was more favorable for blow fly oviposition than prairieland habitat throughout the 2003 study, irrespective of species or time of year. Furthermore, statistical analyses conducted on nocturnal and diurnal temperature ranges furnished evidence to support the hypothesis that 2003 nocturnal temperatures had more of an effect on daily ovipositing than diurnal temperatures for P. eximia and Cynomyopsis cadaverina (Robineau-Desvoidy).

[Truncated abstract] A common application of forensic entomology is the estimation of post-mortem interval (PMI). This is most frequently estimated from the age of calliphorid specimens collected from a corpse, and in many cases it is the immature stages that are encountered. A critical step in the estimation of PMI is the accurate identification of insects to species level, with misidentification potentially resulting in the application of unsuitable developmental data and therefore inaccuracy in the resulting estimate. Identification has long been attempted on a morphological basis, but complicated by the lack of larval keys to the Calliphoridae, limited diagnostic features in immature stages and the poor preservation of specimens. Standard practice in forensic entomology is the rearing of immatures collected from the corpse through to the more distinctive adult stages, however this process is time-consuming and may be hindered where specimens die during rearing. Furthermore, many cases are presented for forensic entomologist as an afterthought and specimens are already preserved. Consequently, a new approach to the identification of calliphorids is sought which will overcome the problems of the morphological and rearing methods. ... The culmination of this study is the consideration of applications of molecular data to forensic entomology. A sequence-specific priming (SSP) technique is presented for the identification of the forensically significant calliphorids of Australia and New Zealand, along with a new method for the extraction and storage of calliphorid DNA samples using Whatman FTA cards. These techniques will potentially improve the efficiency and accuracy of identification in the estimation of PMI using calliphorids. The use of calliphorid DNA is not limited to PMI estimation, but may also be applied to museum studies. DNA was extracted from pupal casings from 300 year old mummified corpses, however difficulty was encountered in amplifying the DNA reproducibly. This illustrates however, the wide-ranging implications of the calliphorid sequence data gathered in this study. This thesis makes a significant contribution to the consideration of the status of some global calliphorid species. The new technique presented for identification of Australian and New Zealand species is the culmination of an important body of data that will ultimately contribute to the strong foundation of forensic entomology and our future accuracy, efficiency and utility as a routine investigative tool.

Made available in DSpace on 2014-06-11T19:22:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2009Bitstream added on 2014-06-13T19:49:19Z : No. of bitstreams: 1 galindo_la_dr_botib.pdf: 1002192 bytes, checksum: efafdb9e0e1cd36e8c2d273acc8df569 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / As interações interespecíficas em dípteros califorídeos de importância forense resultam de processos biológicos complexos, envolvendo comportamentos específicos capazes de influenciar tanto a abundância como a diversidade de insetos presentes nos substratos orgânicos em decomposição. Nesse sentido, a presença de determinadas espécies com forte ação interativa, como por exemplo, predadores, competidores ou parasitóides, pode interferir substancialmente na composição da fauna decompositora de cadáveres, com sérias implicações para a estimativa de intervalo pós-morte em investigações criminais. Em estudo prévio foi investigado em laboratório o comportamento de oviposição em L. eximia, C. megacephala e C. albiceps na tentativa de esclarecer se a postura de ovos em moscas-varejeiras ocorre meramente ao acaso, ou se a presença prévia de diferentes espécies pode influenciar a desova por parte das espécies que chegaram ao local subsequentemente. Os resultados encontrados indicam que as espécies L. eximia e C. megacephala escolhem sítios de oviposição em que não há a presença prévia de larvas de C. albiceps, uma espécie predadora intraguilda. Esses resultados sugerem estudos mais aprofundados, preferencialmente em populações naturais, focalizando sobre a interação entre larvas e adultos de moscas-varejeiras, já que há evidências de que fêmeas adultas são capazes de detectar a presença de larvas predadoras, o que influi na decisão oviposicional. Este estudo teve o objetivo geral de investigar a abundância, diversidade e dinâmica comportamental em populações de moscas-varejeiras no contexto de recursos alimentares efêmeros e limitados, considerando a ação da predação intraguilda. A proposta do estudo é criar duas frentes de investigação no contexto de ecologia comportamental, experimentação em campo e modelagem matemática populacional / The interspecific interactions in calliphorid flies of forensic impoltance result of complex biological processes, involving specific behaviors capable of influencing both the abundance and the diversity of insects in organic substrates in decomposition. The presence of certain species with a strong interactive action, such as predators, competitors or parasitoids, can interfere substantially in the composition of the decomposer fauna of corpses, with serious implications for the postmortem interval estimate in criminal inquiries. In a previous study the oviposition behavior in L. eximia, C. megacephala and C. albiceps was investigated in laboratory in the attempt to clarify if the egg laying in blowflies occurs at random, or if the previous presence of different species may influence the oviposition of the species that had arrived subsequently at the substrate. The results suggest that the species L. eximia and C megacephala prefer oviposition substrates, without the previous presence of C. albiceps larvae, an intraguild predator species. These results suggest further studies, preferably in natural populations, focusing on the interaction between larvae and adult of blowflies, since there is evidence that females are able to detect the presence of predator. This study had the general objective of investigating the abundance, diversity and behavioral dynamic in populations of blowflies in the context of limited and ephemeral food resources, considering the effect of intraguild predation. The proposal of the study is to create two fronts of investigation in the context of behavioral ecology, experimentation in field and population mathematical modeling