Made available in DSpace on 2014-06-11T19:22:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-02-25Bitstream added on 2014-06-13T20:48:30Z : No. of bitstreams: 1 rosa_ra_me_sjrp.pdf: 528158 bytes, checksum: 87eb91b0d9f48ee60092159a596eccf5 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Neste trabalho vamos estudar a existência e unicidade de solução para equações da forma { u + Au = f(t,u) u(t0)= u0 ∈ X, (I) onde X é um espaço de Banach, A : D(A) ⊂ X → X é um operador linear, f é uma função não linear conhecida, u0 ∈ X é um dado inical conhecido e u : I ⊂ R → X é uma função desconhecida e t0 ∈ I. Faremos este estudo usando a Teoria dos Semigrupos de Operadores Lineares. Para melhor entendimento do estudo das equações (I), faremos duas aplicações. A primeira tratando de um modelo (linear) de divisão celular e a segunda, do modelo (não linear) de condução do calor. / In this work we will study the existence and uniqueness of the solutions for the following equation { u + Au = f(t,u) u(t0)= u0 ∈ X, (I) where X is a Banach space, A : D(A) ⊂ X → X is a linear operator, f is a nonlinear function, u : I ⊂ R → X is unknown function. In this study we will use the theory of semigroup of linear operators. For a best understanding of the study of equations (I), we will do two applications. The first one, is a (linear) model of cellular division and the second one, is about the (nonlinear) model od conduction of the heat.

Orientador: Germán Jesus Lozada Cruz / Banca: Marcos Roberto Teixeira Primo / Banca: Andréa Cristina Prokopezyk Arita / Resumo: Neste trabalho vamos estudar a existência e unicidade de solução para equações da forma { u + Au = f(t,u) u(t0)= u0 ∈ X, (I) onde X é um espaço de Banach, A : D(A) ⊂ X → X é um operador linear, f é uma função não linear conhecida, u0 ∈ X é um dado inical conhecido e u : I ⊂ R → X é uma função desconhecida e t0 ∈ I. Faremos este estudo usando a Teoria dos Semigrupos de Operadores Lineares. Para melhor entendimento do estudo das equações (I), faremos duas aplicações. A primeira tratando de um modelo (linear) de divisão celular e a segunda, do modelo (não linear) de condução do calor. / Abstract: In this work we will study the existence and uniqueness of the solutions for the following equation { u + Au = f(t,u) u(t0)= u0 ∈ X, (I) where X is a Banach space, A : D(A) ⊂ X → X is a linear operator, f is a nonlinear function, u : I ⊂ R → X is unknown function. In this study we will use the theory of semigroup of linear operators. For a best understanding of the study of equations (I), we will do two applications. The first one, is a (linear) model of cellular division and the second one, is about the (nonlinear) model od conduction of the heat. / Mestre

Made available in DSpace on 2014-08-20T13:25:41Z (GMT). No. of bitstreams: 1 Tese_ Maribel_da_Silva_Mendes.pdf: 1278110 bytes, checksum: cd3b4973dbfd97e88a02745cadf7c130 (MD5) Previous issue date: 2008-08-29 / The environmental pollution with trace-elements (ETs) is an increasing problem in the modern society, having extreme importance the evaluation of these environmental risks. The use of seeds of superior plants is ideal for such tests since they are efficient, quick and of easy execution. The objectives of this work were evaluate the toxic effects on the germination and cytotoxics on the meristematics cells of Allium cepa L. and Lactuca sativa L. roots, under different concentrations of the trace-elements cadmium (0,5; 1,0; 3,0; 5,0; 7,0 and 9,0mg. L-1), arsenic (2,5; 5,0; 7,5; 10; 15; 20mg. L-1), lead (50, 100, 150, 200, 250 and 300mg. L-1), chromium (50, 100, 150, 200, 250 and 300mg. L-1) and mercury (0,25; 0,5; 1,0; 1,5; 2,0 and 2,5mg. L-1), after 168 and 48h of exhibition, respectively. In lettuce, de maximum number and classes of nucleolus per interphasic cell were observed. The results showed toxic effect of the traceelements on the germination of the seeds and Mitotic Index (IM), besides the induction of chromosomic aberrations in the meristematic cells of A. cepa L. The degree of toxicity and the different anomalies increased with the increase of ET concentration. In L. sativa it was evident that both the percentage of germination and the IM decreased with the increase of ETs concentration. The cytotoxicity caused by these ETs was demonstrated in the different chromosomic anomalies caused to the meristematic cells of this vegetable species. The presence of chrome, even in the lowest concentrations used in this study, impaired the events sequence of the germination process of lettuce seeds. Regarding the number of nucleolus, the studies demonstrated that, the lettuce has, at most, six (6) nucleolus per interphasic cell, even in the presence of the studied trace-elements. / A poluição ambiental com elementos-traço (ETs) é um problema cada vez maior da sociedade moderna, sendo a avaliação destes riscos ambientais de extrema importância. O uso de sementes de plantas superiores é ideal para tais ensaios porque são eficientes, rápidos e de fácil execução. Os objetivos deste trabalho foram avaliar os efeitos tóxicos na germinação e citotóxicos nas células meristemáticas das raízes de Allium cepa L. e de Lactuca sativa L. sob diferentes concentrações dos elementos-traço cádmio (0,5; 1,0; 3,0; 5,0; 7,0 e 9,0mg.L-1), arsênio (2,5; 5,0; 7,5; 10; 15; 20mg.L-1), chumbo (50, 100, 150, 200, 250 e 300mg.L-1), cromo (50, 100, 150, 200, 250 e 300mg.L-1) e mercúrio (0,25; 0,5; 1,0; 1,5; 2,0 e 2,5mg.L-1), após 168 e 48h de exposição, respectivamente. Observouse, em alface, o número máximo e as classes de nucléolos por célula interfásica. Os resultados mostraram efeito tóxico dos elementos-traço sobre a germinação das sementes e no Índice Mitótico (IM), além da indução de aberrações cromossômicas nas células meristemáticas de A. cepa. O grau de toxicidade e as diferentes anomalias aumentam com o aumento da concentração do ET. Em L. sativa ficou evidenciado que tanto a percentagem de germinação, como o IM decresceram com o aumento da concentração dos ETs. A citotoxicidade causada por esses ETs ficou demonstrada nas diferentes anomalias cromossômicas causadas às células meristemáticas dessa espécie vegetal. A presença de cromo, mesmo nas concentrações mais baixas utilizadas neste estudo, inviabilizou a seqüência dos eventos do processo de germinação das sementes de alface. Em relação ao número de nucléolos, os estudos demonstraram que, a alface possui, no máximo, seis (6) nucléolos por célula interfásica, mesmo na presença dos elementos-traço estudados.

La localisation des ARNm par transport dirigé joue un rôle dans le développement, la motilité cellulaire, la plasticité synaptique et la division cellulaire asymétrique. Chez la levure Saccharomyces cerevisiæ, la localisation d’ARNm est un phénomène dont les mécanismes de régulation sont conservés auprès de nombreux autres organismes. Lors de la division de la levure, plus d’une trentaine de transcrits sont localisés par transport actif à l’extrémité du bourgeon de la cellule-fille. Parmi ceux-ci, l’ARNm ASH1 est le mieux caractérisé et constitue le modèle utilisé dans cette étude. Pour exercer sa fonction, la protéine Ash1 doit être produite uniquement après la localisation de l’ARNm ASH1. Pour ce faire, les mécanismes de régulation de la traduction de l’ARNm ASH1 empêchent son expression durant le transport. Ce projet de recherche vise à étudier les mécanismes de régulation de la traduction de l’ARNm ASH1 par les répresseurs traductionnels connus, soit Khd1, Puf6 et Loc1. Les études antérieures se sont penchées sur ces facteurs de manière individuelle. Cependant, dans cette étude, nous avons exploré la présence d’une collaboration entre ceux-ci. Ainsi, nous avons voulu déterminer si les répresseurs traductionnels peuvent être intégrés en une seule voie de régulation de la traduction de l’ARNm ASH1. De plus, nous avons cherché à identifier le mécanisme de recrutement des répresseurs traductionnels sur l’ARNm ASH1, qui correspond au point initial des voies de régulations de l’ARNm ASH1. Nos résultats montrent que les répresseurs traductionnels de l’ARNm ASH1, soit Khd1 et Puf6, font partie d’une même voie de régulation de la traduction. Le rôle du facteur nucléaire Loc1 dans la voie de régulation de la traduction, quant à elle, a été examinée à partir d’expériences permettant l’étude du mécanisme de recrutement des répresseurs traductionnels dans le noyau. Ainsi, nos travaux montrent que Puf6 et Loc1 sont associés de manière ARN-dépendant avec la machinerie de transcription, notamment au facteur d’élongation de la transcription Spt4-Spt5/DSIF. Par ailleurs, notre laboratoire a précédemment montré que la localisation nucléaire de la protéine de liaison à l’ARN She2 est essentielle au recrutement des facteurs Loc1 et Puf6 sur l’ARNm ASH1. Des expériences d’immunoprécipitation de la chromatine (ChIP) supportent l’hypothèse que le recrutement de Loc1 est essentiel à celui de Puf6, qui s’effectue ultérieurement. Ainsi, à partir des résultats de cette étude et des résultats publiés précédemment dans notre laboratoire, nous avons élaboré un modèle de recrutement coordonné des facteurs She2, Loc1 et Puf6 sur l’ARNm ASH1 naissant. De manière générale, cette étude a permis d’établir la présence d’une seule voie de régulation de la traduction de l’ARNm ASH1 et une meilleure connaissance du recrutement des facteurs de répression traductionnelle sur celui-ci. / Directed transport mRNA localization play a role in the development, the cell motility, the synaptic plasticity and asymmetric cellular division. In the yeast Saccharomyces cerevisiæ, this regulation mechanism is conserved among many other species. During yeast cell division, around thirty mRNA are actively localized at the bud tip in the daughter cell. ASH1 mRNA is the best known among them and constitutes the model used in this study. In this model, Ash1 expression is possible only after proper localization of its mRNA. In order to do so, ASH1 mRNA translation is repressed by translational repressors during its active transport. This project investigates the mechanism of ASH1 mRNA translational regulation that is carried out by the translational repressors Khd1, Puf6 and Loc1. Previous studies characterized the action of these factors individually. However, in this study, we now explored the possibility of a collaboration between them. Thus, we sought to determine if these translational repressors are part of the same ASH1 mRNA translational regulation pathway. In addition, we tried to identify the mechanisms of recruitment of these translational repressors on ASH1 mRNA, the molecular mechanisms that initiates this process. In this work, we show that the cytoplasmic translational repressors Khd1 and Puf6 are part of the same ASH1 mRNA translational regulation pathway. In this pathway, the role of the nuclear translational factor Loc1 was determined by the analysis of translational factors recruitment on ASH1 in the nucleus. We demonstrate that Puf6 and Loc1 interact in an RNA-dependent manner with the transcription machinery, via the transcription elongation factor Spt4-Spt5/DSIF. Finally, chromatin immunoprecipitation (ChIP) assays support the model that Loc1 recruitment to nascent ASH1 mRNA is essential for the subsequent recruitment of Puf6 to this transcript. With the results of this study and others previously done in the lab, we elaborated a recruitment model for the She2, Loc1 and Puf6 proteins on the nascent ASH1 mRNA. In conclusion, this study has established that the translational repressors Khd1, Puf6 and Loc1 are part of the same ASH1 mRNA translational regulation pathway and allowed a better understanding of the mechanism of recruitment of translational repressors on their target mRNA.