Genetic code expansion allows the incorporation of non-canonical amino acids with a variety of new functional groups: fluorescent amino acids,1-3 azides,4-6 alkynes,5-10 and photocrosslinkers.4,11,12 This incorporation requires the evolution of new tRNA/aminoacyl tRNA sythetase pairs. Traditionally screenings of novel tRNA/aminoacyl tRNA synthetase pairs have been done in vivo. While these in vivo screenings have proven robust, they are limited in multiple ways: non-canonical amino acids (ncAAs) must be nontoxic and bioavailable. Furthermore, library size is limited by transformation efficiency. Lastly, in vivo screenings require substantial amounts of the target ncAA, which is often not available in large masses. In vitro screenings bypass these limitations: toxicity and bioavailibilty are no longer concerns. Library size can be expanded by several orders of magnitude as we are no longer limited by transformation efficiency. Lastly, because in vitro transcription/translation reactions are routinely conducted on the μL scale, ncAA usage can be minimized. We set out to use in vitro compartmentalization to further expand the code. In an in vitro compartmentalization screening, the water droplets in a water-in-oil emulsion serve as separate reaction chambers in which individual library members are transcribed and translated. Here we report optimization of S30 transcription/translation reactions. Optimizations include cell lysis method, reaction temperature, template amount, and T7 RNA polymerase amounts. Yields remained low and we transistioned into the use of PURExpress. Fluorohistidines are isosteric with histidine, but not isoelectronic.13 This change in environment results in a reduction of pKa. We set out to synthesize 4-fluorohistidine to use as a pH probe in several target proteins. A synthesis of 4-fluorohistidine was published in 1973.14,15 We were able to improve upon this synthesis by reducing cost and improving yield of a key step in the reaction. Next, small peptides with polyhistidine tags were translated in vitro using our 4-fluorohistidine. We are calling this polyhistidine tag incorporating 4-fluorohistidine our “hexafluorohistag.” Because of the reduced pKa of the 4-fluorohistidine, the hexafluorohistag showed affinity to Nickel-NTA resin even at reduced pH. This allowed for the purification of hexafluorohistagged peptides in the presence of traditional polyhistidine-tagged peptides.

L'inflammation systémique est une réaction qui implique l’ensemble de l’organisme suite une agression sévère, potentiellement mortelle, illustrée par le syndrome de réponse inflammatoire systémique (SIRS). De nombreux acteurs cellulaires et moléculaires contribuent au développement de cette cascade inflammatoire parmi lesquels les cellules NK jouent un rôle clé. Malgré l'accumulation de preuves sur l’existence de propriétés spécifiques à chaque organe en réponse à l'inflammation systémique, en termes de cellules NK, on sait peu de choses sur la dynamique compartimentalisée de l’activation des cellules NK pendant un SIRS. En outre, le statut immunitaire des cellules NK après la résolution d’un SIRS est également mal connu. Dans le présent travail, nous avons étudié les réponses des cellules NK provenant de différents organes en utilisant un modèle d’endotoxinémie murine. Nous avons caractérisé la réponse des cellules NK au sein de la rate, du poumon, de la moelle osseuse, de la cavité péritonéale, et dans la circulation. Nous avons trouvé que, malgré une dynamique similaire de la réponse dans les différents organes, les réponses des cellules NK sont compartimentalisées avec des seuils différent et spécifiques. A l’aide de transferts adoptifs, nous avons constaté que la réactivité des cellules NK spécifiques d'organes peut refléter le compartiment d’origine lors des phases initiales de l'inflammation. Cependant, les cellules NK ont la capacité de s’adapter rapidement à leur nouvel environnement et d'ajuster leurs niveaux de réponse à ceux des cellules NK résidentes. Ainsi, cette étude fournit une preuve de concept qui confirme la compartimentalisation de la réponse des cellules NK lors de l'inflammation systémique. Dans une deuxième partie, nous avons analysé le statut des cellules NK à différents moments après une endotoxinémie. Les réponses des cellules NK au sein d’une préparation de cellules de la rate sont fortement supprimées en réponse à une restimulation in vitro, 14 jours après l'endotoxinémie. Cependant, nous avons montré que la réactivité intrinsèque des cellules NK est en fait augmentée après l'injection d’endotoxine, aboutissant à des cellules NK présentant des caractéristiques de cellules NK mémoires. Des expériences de transfert adoptif ont confirmé les propriétés de mémoire des cellules NK après endotoxinémie. Nos résultats accroissent la connaissance concernant le rôle des cellules NK dans un contexte d'inflammation systémique, révélant des réponses compartimentalisés et l’induction d’une mémoire suite à l’endotoxinémie. L'observation selon laquelle les cellules NK développent des propriétés de mémoire après une inflammation systémique dans le contexte d'un environnement suppressif est d’une grande nouveauté et ce phénomène est rapporté pour la première fois. / Systemic inflammation is whole-body reaction to a triggering insult that often results in life threatening illness like systemic inflammatory response syndrome (SIRS). Contributing to the development of this inflammatory cascade are numerous cellular and molecular players, among which, NK cells have been shown to play a key role. Despite accumulating evidence on the organ-specific properties of both systemic inflammation and NK cells, little is known about the compartmentalized dynamics of NK cell activation during SIRS. Furthermore, the status of NK cells after the resolution of SIRS is also poorly characterized. In the present work, we investigated NK responses in different organs using a mouse model of endotoxinemia and characterized the compartmentalized response of spleen, lung, bone marrow, peritoneal and circulating NK cells. We found that despite similar dynamics of response in different organs, NK cells responses, are compartmentalized with seemingly specific thresholds of maximum activation. Using a series of adoptive transfers, we found that while organ-specific NK cell responsiveness can affect the initial phases of inflammation, these cells have the capacity to quickly adapt to a new environment and adjust their response levels to that of resident NK cells. Thus, this study provides proof of concept data on the compartmentalization of the NK cell responses during systemic inflammation. In a second part, we assessed the status of NK cells at different times after endotoxemia. NK cells responses in the context of whole spleen preparations were severely suppressed in response to in vitro restimulation at 14 days after endotoxemia. However, intrinsic NK cell responsiveness was increased after endotoxemia, showing characteristics of NK cell memory. Adoptive transfer experiments confirmed memory properties of NK cells after endotoxemia. Overall, these results expand on the role of NK cells in the context of systemic inflammation revealing compartmentalized responses during and memory properties following endotoxemia. The observation that NK cells develop memory properties after systemic inflammation in the context of a suppressive environment is of the highest novelty and the first one to report such a phenomenon.

A l’ère de l’objectif cible « 90-90-90 » de l’ONUSIDA de réduction de la pandémie liée à HIV, il est important d’évaluer régulièrement la cascade de prise en charge des personnes vivant avec le virus HIV afin d’en vérifier l’avancement et d’identifier d’éventuels obstacles à sa réalisation. Pour cela nous avons étudié tout d’abord efficacité du traitement antirétroviral (TAR) dans le sang de personnes nouvellement dépistées séropositives à Bamako (Mali). Dans un deuxième travail nous avons évalué la faisabilité des tests de charge virale et de résistance génotypique aux antirétroviraux à partir de sang total séché sur un support de type buvard (DBS). La troisième partie de nos travaux était consacrée à des aspects plus physiopathologiques avec l’évaluation du traitement sur les réservoirs salivaires et génitaux (patients de Bamako) et sur le microbiote vaginal, ainsi que l’étude du profil de résistance des souches archivées dans l’ADN cellulaire de biopsies rectales. Nous avons tout d’abord observé un taux important de perdus de vue à un an dans la cohorte de Bamako (environ 45%). Nous avons également constaté un taux élevé de résistance primaire aux ARV à Bamako et au Tchad (>15%). De manière rassurante, le succès virologique au bout de 1 an chez les personnes traitées était d’environ 90% ce qui correspond à l’objectif de l’ONUSIDA (3ème 90) et un faible niveau de mutations acquises a été observé chez ces personnes adhérentes au traitement. Nous avons démontré l’efficacité du TAR sur le compartiment salivaire et constaté une compartimentation du virus au niveau cervico-vaginal chez certaines femmes traitées (27%) présentant une excrétion virale au niveau vaginal avec une charge virale plasmatique indétectable et/ou des séquences génétiques différentes sur le gène pol entre le virus isolé dans la muqueuse et celui provenant du sang. De plus, une dysbiose était observée avant la mise sous traitement, avec normalisation de la flore sous TAR efficace. En ce qui concerne le travail sur les biopsies rectales, des profils similaires ont été observés entre la souche plasmatique majoritaire au moment de la mise sous TAR et celle archivée dans le rectum 1 à 5 ans après traitement. En conclusion, nos travaux apportent des informations nouvelles sur le déroulement des différentes étapes de la prise en charge de l’infection par HIV dans les pays en développement : tout d’abord une faible adhésion au traitement ce qui peut constituer un obstacle majeur à la réalisation du plan 90/90/90 ; une forte prévalence de la résistance primaire qui plaident en faveur de l’accessibilité aux différentes classes d’antirétroviraux et de leur utilisation rationnelle, de l’utilisation généralisée en routine des tests de charge virale et du développement d’un réseau de surveillance de la résistance aux ARV dans les pays à ressources limitées ; des données d’efficacité de traitement sur les réservoirs muqueux mettant en évidence l’existence d’une dysbiose et d’une compartimentation du virus au niveau génital ce qui pose le problème du risque résiduel de transmission chez certaines personnes, même sous ARV. / Regularly assess to UNAIDS cascade 90-90-90 is important to check the progress and identify any obstacles to its implementation. For this we first studied efficacy of antiretroviral treatment (ART) in the blood of newly diagnosed HIV-positive in Bamako (Mali).In a second work we evaluated the feasibility of viral load and genotypic resistance tests from dried blood spot (DBS). The third part of our work is dedicated to pathophysiological aspects with evaluation of treatment on salivary and genital reservoirs (Bamako patients) and on the vaginal microbiota, as well as the study of the resistance profile of the strains archived in cellular DNA of rectal biopsies. We observed a high rate of lost to follow-up at one year in the Bamako cohort (45%). We also found a high rate of ART primary resistance in Bamako and Chad (> 15%). Reassuringly, the virological success after 1 year of treated follow was about 90% in these adherents. We also demonstrated the efficacy of ART in the salivary compartment and found a compartmentalization of the virus at the cervico-vaginal level in some women under ART. In addition, a dysbiosis was observed before ART, and a normal flora under effective ART. Similar profiles were observed on the main strain isolated in blood at the time of diagnosis and on the archived strain in the rectum after 1 to 5 years of ART.In conclusion, our work provides new information on the progress of the treatment stages of HIV infection in developing countries: low adherence to treatment which can constitute a major obstacle to achieve the plan 90/90/90; a high prevalence of primary resistance advocating accessibility and rational use of different classes of antiretrovirals drugs, widespread routine use of viral load tests and the development of ARVs resistance surveillance network in resource-limited countries; treatment efficacy data on mucosal reservoirs revealing the existence of genital dysbiosis and viral compartmentalization, which raises the problem of the residual risk of transmission in some people, even under ARVs.

Unidades climáticas são determinadas áreas da superfície terrestre que apresentam características climáticas relativamente homogêneas ao longo do tempo e do espaço. Sua existência, a priori, depende da relação única entre a superfície terrestre e a dinâmica atmosférica, quando a primeira exerce controle sobre a segunda, influenciando tais características. Essas características climáticas, sob a forma da variação temporal da temperatura, umidade, chuvas, entre outros, são espacialmente melhor percebidas de acordo com a escala de trabalho da pesquisa adotada e podem realçar a existência de ambientes diferenciados (daqueles conhecidos em escalas mais generalistas) - os climas locais e os topoclimas, que são a base climatológica mais complexa dos planos de ordenação territorial. O município de Ilhabela reflete as transformações socioeconômicas e ambientais que vem ocorrendo na região do Litoral Norte do estado de São Paulo, essencialmente no período posterior a 1970, com a inauguração da Rodovia Rio-Santos (BR101/SP55), que pressupõem, por conta do aporte migratório, transformações nas paisagens locais e regionais. Entretanto, a Ilha de São Sebastião (a maior e sede do município) resguarda 75% de seu território sob a forma de unidade de conservação Parque Estadual de Ilhabela (PEIb). Desta forma, a essência do estudo aqui proposto é a identificação das unidades climáticas naturais da ilha, por meio da análise da variação de temperatura, umidade relativa e precipitações identificadas em diferentes localidades deste ambiente ilhéu e avaliar possíveis influências da urbanização sobre este processo. O método utilizado nesta pesquisa está assentado na instalação de equipamentos sensores da temperatura do ar, umidade relativa e da precipitação líquida como os termo-higrômetros (eletrônicos) e os pluviômetros (totalizadores e registradores, manuais e eletrônicos, respectivamente), dentro do período de um ano. A análise dos dados considerou aspectos geográficos e climatológicos de ordem local, assim como aqueles de ordem regional (meteorológicos sinóticos) resultando em informações quantitativas e qualitativas, que sob a luz da análise rítmica e da heurística possibilitaram sua análise crítica. Os resultados, quando observados individualmente, representam a situação geoclimática dos sítios de instalação da instrumentação meteorológica; por outro lado, a variabilidade espacial dos atributos em sua relação com os sistemas atmosféricos atuantes, considerados então como representativos dos meso e topoclimas, permite a identificação, individualização e definição de unidades climáticas da ilha no período da coleta de dados. Foram então definidos 12 ambientes topoclimaticamente diferenciados com a clara percepção de heterogeneidade entre si, organizados sob 5 mesoclimas conforme a insolação, os fluxos de ar predominantes, a orientação geral das vertentes e das bacias hidrográficas e a variação dos atributos do clima. O setor norte: quente e pouco chuvoso. A vertente leste: mais ensolarada e úmida. O sul sudoeste: barlavento dos sistemas frontais. O setor central: urbano é sujeito a atividade convectiva intensa. / Climate units are certain areas of the Earth\'s surface that have relatively homogeneous climatic characteristics over time and space. Its existence, in principle, depends on the unique relationship between the ground surface and the atmospheric dynamics, where the first exercises control over the second influencing such features. These climatic characteristics in the form of temporal variation of temperature, humidity, rainfall, among others, are spatially better perceived according to the adopted research work schedule and can highlight the existence of different environments (those known in more general scales) - local climates and topoclimas, which are the most complex climatological basis of territorial development plans. The municipality of Ilhabela reflects the socioeconomic and environmental changes that have occurred in the North Coast region of the state of São Paulo, mainly in the period to 1970, with the inauguration of the Rio-Santos highway (BR101 / SP55), which require, due to the migratory contribution, changes in local and regional landscapes. However, Sao Sebastiao Island (the largest and county seat) protects 75% of its territory in the form of conservation unit - Ilhabela State Park (PEIb). Thus, the essence of the herein proposed study is to identify the natural climate units of the island, through the analysis of the variation of temperature, relative humidity and precipitation identified in different locations of the islet environment and to evaluate the possible influence of urbanization on this process. The method used in this research is seated in the installation of sensor equipment of the air temperature, relative humidity and liquid precipitation as the thermo-hygrometers (electronic) and rain gauges (totalizers and registers, manual and electronic, respectively) within the period of one year. Data analysis considered geographical and climatological aspects of local order, as well as those of regional order (synoptic weather) resulting in quantitative and qualitative information, which in the light of rhythmic analysis and heuristics enabled critical analysis. The results, when viewed individually, represent the geoclimática situation of installation sites of meteorological instrumentation; on the other hand, the spatial variability of the attributes in its relation to the active atmospheric systems, considered then as representative of meso and topoclimas allows the identification, individualization and definition of climate units of the island in the period of data collection. They were then set 12 topoclimaticamente different environments with a clear perception of heterogeneity among themselves, organized under 5 mesoclimas as heatstroke, prevailing air flows, the general orientation of the slopes and river basins and the change of climate attributes. The northern sector: hot and slightly rainy. The east side: more sunny and humid. The south southwest: windward of frontal system. The central sector: Urban is subject to intense convective activity.

Atom transfer radical polymerization (ATRP) which utilizes transition metal based catalysts is a versatile methodology for the synthesis of a wide spectrum of polymers with controlled architectures. However, high concentrations of soluble catalyst required in an ATRP process makes the final polymer colored and toxic. Thus, the catalyst removal/reduction/recycling remains a challenge in the field of ATRP. Supported catalysts on insoluble solids such as silica gel, polystyrene beads, etc. have been used in ATRP to facilitate the catalyst recovery and recycling. However, the ability of the supported catalysts to mediate a polymerization is substantially reduced due to their reduced mobility and leaching problems. In this thesis, we report a series of novel and recyclable physisorbed CuBr2/N, N, N’, N’’-pentamethyldiethylene-triamine supported catalytic systems operating in conjunction with hydration. Supported aqueous-phase catalysis (SAPC) for ATRP was evaluated for different inorganic (Na-clay, silica and zeolite) and organic (polysaccharides) supports. The hydrated physisorbed supported catalysts were used for the polymerization of benzyl methacrylate and methyl methacrylate using an activator generated electron transfer ATRP process. The catalyst was effectively retained on the surface of supports through hydration as was verified by UV-Vis measurements. The supported catalyst was easily removed from the polymerization by simple filtration process affording a colorless polymer solution. The polymerizations produced high conversion and colorless polymers with moderately narrow polydispersity indices (PDI). The catalyst maintained high activity during the recycling experiments. We also investigated the kinetic and mechanistic behavior of these solid supported polymerization systems. Based on split kinetics experiments and UV-Vis studies it was believed that the activation and deactivation processes took place at the diffused hydrated interface between the solid support and organic phase. The branched (stars and graft) polymers were also synthesized using Na-clay supported catalyst. The produced polymers had narrow PDI and good initiator efficiencies. The functionality of the star polymers was confirmed using 1H NMR and dilute solution properties. The synthesis of graft-copolymer was confirmed by 1H NMR and atomic force microscopy. This thesis demonstrates the successful use of SAPC for ATRP to produce contamination free linear and branched polymers with moderately narrow PDI and high recycling efficiency.

Reservoir fluids are rarely found in homogeneous structures having homogeneous properties. The various elements and processes of the petroleum system result in complex fluid distributions and compositions. A sound understanding of these complexities can avoid disappointing results and costly mistakes when designing the completion and production of the reservoir. The earlier these complexities are understood in the exploration phase, the better are the chances of a successful decision making process in the design phase of the project. Assessing reservoir compartment connectivity is of paramount importance for a optimal field development. Recent technological advances in wireline formation testing and sampling provide asset teams with a new methodology to evaluate in situ fluid properties and reservoir connectivity. After a review of the technology of downhole fluid analysis (DFA), the currently available methods of modeling equilibrated fluid gradients are presented. Fluid composition equilibrium is a stationary state where all components have reached zero mass flux. A reservoir model is designed to simulate numerically equilibration processes over geologic timescales at isothermal conditions where diffusion and gravity are the active mechanisms. A variety of initial conditions and reservoir fluid types is considered. Non-equilibrium fluid gradients and their transient behavior as they evolve towards fluid composition equilibrium are the main interest of this study. The results are compared in case studies, that are available in published literature. The modeling methods allow modeling of vertical and lateral fluid gradients. After a discussion of the cases, this thesis gives recommendations on 1) what fluid properties should be assessed and 2) how many data points are needed to reduce the chance of misinterpretation of non-equilibrium gradients in the presence of faults. To make best use DFA data, the property that exhibits the largest gradient needs to be investigated, as it yields the greatest potential to assess connectivity. The shape of the distribution of fluid composition within a compartment is found to be an important part in investigating reservoir connectivity. During data acquisition efforts should be made to acquire enough data points to reveal this shape. In combination with the presented techniques to identify non-equilibrium conditions, this will optimize DFA data acquisition and maximize the value of the data.

Integration of dynamic data is critical for reliable reservoir description and has been an outstanding challenge for the petroleum industry. This work develops practical dynamic data integration techniques using streamline approaches to condition static geological models to various kinds of dynamic data, including two-phase production history, interference pressure observations and primary production data. The proposed techniques are computationally efficient and robust, and thus well-suited for large-scale field applications. We can account for realistic field conditions, such as gravity, and changing field conditions, arising from infill drilling, pattern conversion, and recompletion, etc., during the integration of two-phase production data. Our approach is fast and exhibits rapid convergence even when the initial model is far from the solution. The power and practical applicability of the proposed techniques are demonstrated with a variety of field examples. To integrate two-phase production data, a travel-time inversion analogous to seismic inversion is adopted. We extend the method via a 'generalized travel-time' inversion to ensure matching of the entire production response rather than just a single time point while retaining most of the quasi-linear property of travel-time inversion. To integrate the interference pressure data, we propose an alternating procedure of travel-time inversion and peak amplitude inversion or pressure inversion to improve the overall matching of the pressure response. A key component of the proposed techniques is the efficient computation of the sensitivities of dynamic responses with respect to reservoir parameters. These sensitivities are calculated analytically using a single forward simulation. Thus, our methods can be orders of magnitude faster than finite-difference based numerical approaches that require multiple forward simulations. Streamline approach has also been extended to identify reservoir compartmentalization and flow barriers using primary production data in conjunction with decline type-curve analysis. The streamline 'diffusive' time of flight provides an effective way to calculate the drainage volume in 3D heterogeneous reservoirs. The flow barriers and reservoir compartmentalization are inferred based on the matching of drainage volumes from streamline-based calculation and decline type-curve analysis. The proposed approach is well-suited for application in the early stages of field development with limited well data and has been illustrated using a field example from the Gulf of Mexico.

Airflow into, out of, and within buildings is fundamental to their design and operation as it can affect occupant health and comfort, building durability, and energy consumption. This thesis works to develop the understanding of airflow patterns and pressure regimes in high-rise multi-unit residential buildings which are both unique and complex due to the combination of their height, typical inclusion of operable windows, and compartmentalized layout. Specific attention is directed towards the performance of corridor pressurization based ventilation systems which are used pervasively within industry to ventilate and control contaminant transfer in these buildings. Airflow is caused by pressure differences which for buildings are created by the driving forces of wind, stack effect, and mechanical ventilation systems. These airflows are resisted by the air permeance (i.e. airtightness) of building elements including the exterior enclosure and interior compartmentalizing elements. Using an experimental program at a case study building, this thesis assesses the interaction of these driving forces of airflow with the physical building to create the airflow patterns for a typical high-rise multi-unit residential building. Perflourocarbon tracer (PFT) testing was performed to measure in-service airflows into and out of the suites. This testing found that the air change rates of upper suites are significantly higher than that of lower suites and that most suites receive small fractions of modern ventilation rates or are over ventilated. Airflow measurements of the supply of ventilation air to each corridor indicate that these low flow rates are in part due to leakage of air from the supply duct. The PFT testing also found that significant airflow occurred from the parking garage below the building into the occupied building spaces indicating significant potential for transfer of harmful air contaminants. The air permeance of the exterior enclosure and interior compartmentalizing elements were measured using neutralized fan pressurization and depressurization techniques and found to be within typical ranges. In particular this testing found that only 20% of the flow paths out of the corridor were to the adjacent suites through the suite entrance doors and that flows to the elevator shaft and stairwells could create a significant inefficiency in the ventilation system. A long-term monitoring program was implemented at the case study building primarily to monitor exterior environmental conditions including wind and exterior temperature and to correlate these with measured pressure differences. A strong correlation was found between building pressure and exterior temperature. Nearly 70% of the theoretical stack effect pressure was measured to act across the corridor to suite pressure boundary which creates a significant pressure differences to be overcome by the ventilation system, likely contributing to the uneven distribution of ventilation rates. Both wind and stack effect pressures were found to often be of similar or greater magnitude than mechanically induced pressure differences and thus can overwhelm the ventilation system. Overall, the corridor pressurization based ventilation system at the case study building does not effectively or efficiently ventilate the building and also does not provide sufficient control of air contaminants. As the case study building was found to be relatively representative of a typical multi-unit residential building, the findings from this building can be extended to many other buildings. Effective ventilation and airflow control in multi-unit residential buildings likely requires suite compartmentalization and direct supply of ventilation via ducted or in-suite systems.

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No. of bitstreams: 1 ferreira_mn_me_rcla.pdf: 8244618 bytes, checksum: f6eec6ad55c4fa070d073f0df07c532a (MD5) Previous issue date: 2017-10-26 / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Considerando o crescente número de acidentes com produtos perigosos em rodovias e que este é o modal mais utilizado para o transporte de cargas no Brasil, principalmente hidrocarbonetos e derivados de petróleo. Bem como, os insipientes estudos de índices de sensibilidade ambiental para ambientes terrestres. E que os mapas de sensibilidade são importantes ferramentas técnico-gerenciais utilizadas na tomada de decisões emergenciais em caso de combate a derrames de óleo. Além de fornecer informações para o planejamento de contenção e limpeza para mitigar os impactos ambientais, auxiliar a identificação de locais sensíveis antes que ocorra o acidente, de modo que as estratégias de proteção e contenção possam ser organizadas antecipadamente, objetiva-se a confecção de um índice de sensibilidade ambiental aos hidrocarbonetos para rodovias, utilizando como área de estudo um trecho da Rodovia Anhanguera (SP-330), segunda maior em números de acidentes com produtos perigosos no estado de São Paulo, e sua respectiva carta de sensibilidade ambiental. Para tanto, procede-se fazendo uso de geotecnologias para confecção de mapas básicos necessários a pesquisa; da compartimentação fisiográfica para dividir a área de estudo em zonas homólogas; do trabalho de campo para corroborar a compartimentação; da escolha dos cinco parâmetros e seus respectivos pesos, que compõem o índice de sensibilidade ambiental aos hidrocarbonetos para rodovias; e consequentemente, a confecção da carta de sensibilidade ambiental. Desse modo, observa-se que no trecho foram encontrados os índices 5, 7 e 8, sendo que o índice 7 é predominante em seis das oito Unidade Básicas de Compartimentação, o que permite concluir que a área estudada tem média-alta sensibilidade ambiental, levando em consideração que o índice vai de 1 a 10 A. Além de colaborar com o desenvolvimento de uma adaptação do método das cartas SAO utilizadas em ambientes costeiros e fluviais para um índice de sensibilidade ambiental aos hidrocarbonetos para rodovias. Por fim, conclui-se que o mapeamento de sensibilidade ambiental aos hidrocarbonetos para o modal rodoviário apresenta-se viável a partir da aplicação de um método adequado ao ambiente em que está inserido e o método da compartimentação fisiográfica foi extremamente apropriado e eficaz para os ambientes terrestres. / Considering the increasing number of accidents with hazardous products on highways and that this is the most used modal for the transport of cargoes in Brazil, mainly hydrocarbons and petroleum products. As well as, the insipient studies of environmental sensitivity indexes for terrestrial environments. And that sensitivity maps are important technical and managerial tools used in making emergency decisions in case of combating oil spills. In addition to providing information for containment and cleanup planning to mitigate environmental impacts, assisting the identification of sensitive locations before the accident occurs, so that protection and containment strategies can be organized in advance, it is aimed to create an Index of environmental sensitivity to hydrocarbons for highways, using as an area of study a section of the Anhanguera Highway (SP-330), second largest in numbers of accidents with dangerous products in the state of São Paulo, and its respective letter of environmental sensitivity. To do so, we make use of geotechnologies to create basic maps necessary for the research; the physiographic compartmentalization to divide the study area into homologous zones; the fieldwork to corroborate the compartmentalization; the choice of the five parameters and their respective weights, which make up the environmental sensitivity index for hydrocarbons for highways; and consequently, the preparation of the environmental sensitivity letter. Therefore, it can be observed that the indexes 5, 7 and 8 were found in this section, with index 7 being predominant in six of the eight Basic Compartmental Units, which allows to conclude that the studied area has medium-high environmental sensitivity, taking into account that the index ranges from 1 to 10 A. In addition to collaborating with the development of an adaptation of the SAO letters used in coastal and fluvial environments to an environmental sensitivity index to hydrocarbons for highways. Finally, it is concluded that the mapping of environmental sensitivity to hydrocarbons for the road modal is feasible from the application of a method appropriate to the environment in which it is inserted and the physiographic compartmentalization method was extremely appropriate and effective for the land environments.

Submitted by BEATRIZ MARQUES GABELINI null (bgabelini@gmail.com) on 2017-08-31T17:47:38Z No. of bitstreams: 1 Dissertação GABELINI, B M 2017.pdf: 19236253 bytes, checksum: f945ee682ee01330adc9122af7b4b3dd (MD5) / Approved for entry into archive by Luiz Galeffi (luizgaleffi@gmail.com) on 2017-08-31T19:36:46Z (GMT) No. of bitstreams: 1 gabelini_bm_me_rcla.pdf: 19236253 bytes, checksum: f945ee682ee01330adc9122af7b4b3dd (MD5) / Made available in DSpace on 2017-08-31T19:36:46Z (GMT). No. of bitstreams: 1 gabelini_bm_me_rcla.pdf: 19236253 bytes, checksum: f945ee682ee01330adc9122af7b4b3dd (MD5) Previous issue date: 2017-06-30 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Os escorregamentos são movimentos de massa recorrentes na Serra do Mar. Quando ocorrem de forma generalizada podem causar prejuízos e perdas de vidas, como no acidente ocorrido no município de Caraguatatuba (SP) em 1967. Com o intuito de evitar estes danos, na literatura, são propostos vários métodos para analisar e prever a ocorrência destes processos. Diante do exposto, o objetivo principal desta pesquisa consiste em analisar a suscetibilidade a escorregamentos na região entre Caraguatatuba e São Sebastião (SP), utilizando a compartimentação fisiográfica e a análise quantitativa. Para alcançar estes objetivos foi realizada a análise e compartimentação do terreno e a análise quantitativa dos fatores condicionantes (declividade, hipsometria e geologia) e da compartimentação fisiográfica. Foram testados, em ambiente SIG, diversos cenários a partir de dois métodos distintos. No Método 1, os fatores condicionantes foram analisados através de pesos de influência. No Método 2, os fatores condicionantes mantiveram os mesmos pesos do Método 1 e foram somados pesos para as classes de cada fator. A compartimentação fisiográfica definiu 16 unidades. Os resultados demonstraram que a maioria dos escorregamentos se concentraram na unidade fisiográfica IV e que há um controle topográfico na distribuição dos escorregamentos entre 100 e 400 m de altitude, em encostas com declividade entre 15º e 30º, compostas por gnaisse migmatítico e granito-gnaisse. Os mapas elaborados a partir do Método 2 apresentaram expressiva concordância com o mapeamento de cicatrizes de Fúlfaro et al. (1976). O cenário escolhido como mais coerente foi o Cenário O, em que houve 60% de influência da declividade, 30% de hipsometria e 10% de geologia. Portanto, a partir do estudo da suscetibilidade, por meio da análise fisiográfica e quantitativa, é possível afirmar que o fator que tem mais influência na ocorrência de escorregamentos, na área de estudo, é a declividade, seguida da hipsometria e geologia. / Landslides are recurrent mass movements in the Serra do Mar. When they occur in a generalized way, as in the event occurred in Caraguatatuba city (SP) in 1967, they can cause a lot of environmental damage and deaths. In order to avoid this damage, several methods were proposed to analyze and predict the occurrence of this process. Considering the above, the main objective of this research is to analyze the susceptibility to landslides in the region between Caraguatatuba and São Sebastião (SP), using physiographic compartmentalization and quantitative analysis. In order to achieve these objectives, a physiographic analysis of the study area was carried out by the method of physiographic compartmentalization. Topographic and geological factors, as well as a physiographic comparison were analyzed using percentages of Class Frequency, Scars Concentration and Disproportionality Factor. We tested several scenarios from two different methods in a GIS environment. Method 1 by which the conditioning factors were analyzed through influence weights and Method 2, which maintains the influence weight for the factors and includes weights to the classes of each factor. The physiographic compartmentalization defined 16 units. Our results showed that most of the landslides were concentrated in the Physiographic Unit IV, and a topographic control in the distribution of landslides between 100 and 400 m of altitude, in slopes with declivity between 15 and 30º, composed of migmatite gneiss and granite-gneiss. Method 2 presented significant agreement with the scar mapping of Fúlfaro et al. (1976), and the scenario chosen as more coherent was Scenario O, which presented 60% influence of declivity, 30% hypsometry and 10% geology. The application of the different methods was satisfactory and based on the results we considered the utilization of the physiographic compartmentalization and the probabilistic analysis as important tools in the prediction of susceptibility, thus being able to collaborate with the territorial and environmental management of the cities.