Analysis of the genome of Anabaena sp. PCC7120 reveals that it lacks the gene, ccaA, which encodes the bonafide carboxysomal, β-class carbonic anhydrase (CA) CcaA. However, the carboxysome enriched fraction of Anabaena PCC7120 exhibits CA activity. Bioinformatic analysis reveals that the N-terminal region of the carboxysome protein CcmM has high sequence and structural similarity to the γ-class CA of Methanosarcina thermophila. Recombinantly expressed CcmM is found to be inactive in in-vitro CA assays. E. coli cell extracts containing an overexpressed form of CcmM comprised of the N-terminal 209 amino acids (CcmM209) are also inactive. However, CcmM209 displays CA activity after incubation with the thiol oxidizing agent diamide or when bound to an affinity matrix. It appears that CcmM is indeed a functional γ-CA which is active under oxidizing condition. It is hypothesized that the C-terminal RbcS like domain in CcmM may regulate activity by allowing CcmM activation only when sequestered within the carboxysome.

Concentrating Photovoltaic systems are good candidates for low cost and clean electricity generation from solar energy. CPV means replacing much of the expensive semiconductor photovoltaic cells with the cheaper optics. Although the idea is simple, CPV systems have several problems inherent to their system design, such as module thickness, expensive PV cells and overheating. Light guide systems are good alternatives to classical CPV systems that can clear off most of the problems of those systems. In this thesis we explore a new light-guide based solar concentrator by optical design and simulations. It is shown that this solar concentrator can reach 1000x geometric concentration, 96.5% optical efficiency with a &plusmn / 1 degree acceptance angle. As a result of simulations, effectiveness of the horizontally staggered light guide solar concentrators is proved. A practical module study is carried on to improve the knowledge related to light guide CPV systems. The concentrator geometry is fabricated as a medium concentrator system with a geometric concentration of 45x and +-2 degrees acceptance angle. With the prototype level injection molding 74% optical efficiency is achieved and can be improved with a better mold manufacturing. A cost analyses is also performed with real manufacturing parameters and it is shown that grid parity can be achieved with this kind of light guide solar concentrators.

The Department of Energy funded a project to determine if the specific heat of thermal energy storage materials could be improved by adding nanoparticles. The standard thermal energy storage materials are molten salts. The chosen molten salt was a sodium nitrate and potassium nitrate eutectic, commercially called Hitec Solar Salt. Two nanoparticle types were chosen, alumina and silica. The nanoparticle composite materials were fabricated by mixing the components in an aqueous solution, mixing that solution for a set amount of time using a sonic mixer, then removing the water from the aqueous solution, leaving the composite molten salt behind as a fine white powder. The thermal properties of the composite and plain material were measured using two techniques: American Society for Testing and Materials (ASTM) 1269E and Modulating Differential Scanning Calorimetry (MDSC). These two techniques measured the specific heat and the heat of fusion of the plain and composite materials. The results of all the ASTM and MDSC measurements suggest that the addition of the nanoparticles using the given manufacturing technique increased the specific heat of the molten salt by approximately 20 percent, with both measurement techniques showing approximately the same level of increase. The silica and the alumina improved the specific heat by nearly the same amount over the base material. The heat of fusion did not seem to be significantly altered compared to the observed heat of fusion value of the unmodified material. It was also observed that the nitrate and silica composite material's specific heat decreased if the material was raised to a temperature above 400C. The specific heat was observed to decrease over time, even when the temperature was well below 400C. It is unknown why this occurred. The nitrate plus alumina composite and the plain nitrate were stable to a temperature of 450C for the test duration.

In this study, the theoretical performance of a concentrating solar thermal electric system (CSTES) using a field of parabolic trough collectors (PTC) is investigated. The commercial software TRNSYS and the Solar Thermal Electric Components (STEC) library are used to model the overall system design and for simulations. The model was constructed using data from the literature for an existing 30-MW solar electric generating system (SEGS VI) using PTC&rsquo / s in Kramer Junction, California. The CSTES consists of a PTC loop that drives a Rankine cycle with superheat and reheat, 2-stage high and 5-stage low pressure turbines, 5-feedwater heaters and a dearator. As a first approximation, the model did not include significant storage or back-up heating. The model&rsquo / s predictions were benchmarked against published data for the system in California for a summer day. Good agreement between the model&rsquo / s predictions and published data were found, with errors usually less than 10%. Annual simulations were run using weather data for both California and Antalya, Turkey. The monthly outputs for the system in California and Antalya are compared both in terms of absolute monthly outputs and in terms of ratios of minimum to maximum monthly outputs. The system in Antalya is found to produce30 % less energy annually than the system in California. The ratio of the minimum (December) to maximum (July) monthly energy produced in Antalya is 0.04.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2009. / Härtill 4 uppsatser.

Abstract The gammaproteobacterium Thiomicrospira crunogena XCL–2 is a hydrothermal vent chemolithoautotroph that has a carbon concentrating mechanism (CCM), which is functionally similar to that of cyanobacteria. At hydrothermal vents, dissolved inorganic carbon (DIC) concentrations and pH values fluctuate over time, with CO2 concentrations ranging from 20 μM to greater than 1 mM, therefore having a CCM would provide an advantage when CO2 availability is very low as CCMs generate intracellular DIC concentrations much higher than extracellular, thereby providing sufficient substrate for carbon fixation. The CCM in T. crunogena includes α–carboxysomes (intracellular inclusions containing form IA RubisCO and carbonic anhydrase), and also presumably requires at least one active HCO3 µ transporter to generate the elevated intracellular concentrations of DIC. To determine whether RubisCO itself might be adapted to low CO2 concentrations, the KCO2 for purified carboxysomal RubisCO was measured (250 μM SD ±; 40) and was much greater than that of whole cells (1.03 μM). This finding suggests that the primary adaptation by T. crunogena to low–DIC conditions has been to enhance DIC uptake, presumably by energy–dependent membrane transport systems that are either ATP–dependent and/or dependent on membrane potential (δ ψ). To determine the mechanism for active DIC uptake, cells were incubated in the presence of inhibitors targeting ATP synthesis andδ ψ. After separate incubations with the ATP synthase inhibitor DCCD and the protonophore CCCP, intracellular ATP was diminished, as was the concentration of intracellular DIC and fixed carbon, despite the absence of an inhibitory effect on δ ψ in the DCCD–incubated cells. In some organisms, DCCD inhibits the NDH–1 and bc1 complexes so it was necessary to verify that ATP synthase was the primary target of DCCD in T. crunogena. Both electron transport complex activities were assayed in the presence and absence of DCCD and there was no significant difference between inhibited (309.0 μmol/s for NDH–1 and 3.4 μmol/s for bc1) and uninhibited treatments (271.7 μmol/s for NDH–1 and 3.6 μmol/s for bc1). These data support the hypothesis that an ATP–dependent transporter is primarily responsible for HCO3 µ transport in T. crunogena. The ATP–dependent transporter solute–binding protein gene (cmpA) from Synechococcus elongatus PCC 7942, was used to perform a BLAST query. Tcr_1153 was the closest match in the T. crunogena genome. However, the gene neighborhood and the result of a maximum likelihood tree suggest that Tcr_1153 is a nitrate transporter protein. Work is underway to find the genes responsible for this ATP–dependent transporter.

A new receiver concept utilizing impinging jet cooling technology has been developed for a small scale solar dish-Brayton system. In a typical impinging receiver design, the jet nozzles are distributed evenly around the cylindrical absorber wall above the solar peak flux region for managing the temperature at an acceptable level. The absorbed solar irradiation is partially lost to the ambient by radiation and natural convection heat transfer, the major part is conducted through the wall and taken away by the impingement jets to drive a gas turbine. Since the thermal power requirement of a 5 kWe Compower® micro gas turbine (MGT) perfectly matches with the power collected by the EuroDish when the design Direct Normal Irradiance (DNI) input is 800 W/m2, the boundary conditions for the impinging receiver design in this work are based on the combination of the Compower®MGT and the EuroDish system. In order to quickly find feasible receiver geometries and impinging jet nozzle arrangements for achieving acceptable temperature level and temperature distributions on the absorber cavity wall, a novel inverse design method (IDM) has been developed based on a combination of a ray-tracing model and a heat transfer analytical model. In this design method, a heat transfer model of the absorber wall is used for analyzing the main heat transfer process between the cavity wall outer surface, the inner surface and the working fluid. A ray-tracing model is utilized for obtaining the solar radiative boundary conditions for the heat transfer model. Furthermore, the minimum stagnation heat transfer coefficient, the jet pitch and the maximum pressure drop governing equations are used for narrowing down the possible nozzle arrangements. Finally, the curves for the required total heat transfer coefficient distribution are obtained and compared with different selected impinging arrangements on the working fluid side, and candidate design configurations are obtained. Furthermore, a numerical conjugate heat transfer model combined with a ray-tracing model was developed validating the inverse design method and for studying the thermal performance of an impinging receiver in detail. With the help of the modified inverse design method and the numerical conjugate heat transfer model, two impinging receivers based on sintered α-SiC (SSiC) and stainless steel 253 MA material have been successfully designed. The detailed analyses show that for the 253 MA impinging receiver, the average air temperature at the outlet and the thermal efficiency can reach 1071.5 K and 82.7% at a DNI level of 800 W/m2 matching the system requirements well. Furthermore, the local temperature differences on the absorber can be reduced to 130 K and 149 K for two different DNI levels, which is a significant reduction and improvement compared with earlier published cavity receiver designs. The inverse design method has also been verified to be an efficient way in reducing the calculation costs during the design procedure. For the validation and demonstration of the receiver designs, a unique experimental facility was designed and constructed. The facility is a novel high flux solar simulator utilizing for the first time Fresnel lenses to concentrate the light of 12 commercial high power Xenon-arc lamps. Finally, a prototype of a 253 MA based impinging was experimentally studied with the help of the 84 kWe Fresnel lens based high flux solar simulator in KTH. / <p>QC 20151123</p> / Optimised Microturbine Solar Power System , OMSOP

Salinity usually reduces plant growth in terms of height and biomass, but can increase secondary metabolite production. This frequently reported observation in guayule (Parthenium argentatum Gray, Asteraceae) was investigated for possible mechanisms.Osmotic and specific ion effects of four chloride salts (CaCl2, MgCl2, KCl, and NaCl) on leaf anatomical and plant physiological parameters were studied. One-year-old plants of guayule line AZ 2 were grown under two salt concentrations (750 ppm and 1500 ppm) for each salt type (plus a control) in sand culture (semi-hydroponic) for eight weeks under controlled greenhouse conditions in Tucson, Arizona.Growth in height decreased with increasing salt concentration. Shoot dry weight, rubber, and resin contents, however, showed no significant differences between treatments, indicating no effect from either salt concentration or salt type. There was a trend for increasing rubber content with increasing salt concentration, although not statistically significant. At the same time, net CO2 gas exchange rates decreased significantly with increasing salinity.With increasing salt concentration, guayule showed osmotic effects in terms of height, indicating a lower hydraulic conductivity. Although plants of higher salt concentrations utilized significantly less water, they had the same shoot dry weights, rubber, and resin contents. Salt-stressed plants therefore achieved higher water use efficiencies. The diurnal net CO2 gas exchange rates were significantly reduced with increasing salinity; the nocturnal net CO2 gas exchange rates showed no significant difference between the treatments.Anatomically, it was found that the stomata were raised or elevated above the epidermis, and supported by upwardly curving cells. When guayule was grown under salt treatments, the trichomes were found to include deposits of material. Trichomes might act as a detoxification repository for excess ions. Although the physiological significance of raised stomata is unknown, it is hypothesized that the unique combination of raised stomata, indumentum, and multiple layers of palisade parenchyma allows for an overall high photosynthetic capacity and performance. During stress conditions such as salinity or drought, guayule might activate an internal CO2 concentrating mechanism, i.e., bicarbonate/CO2 pump, internal CO2 recycling, or PEP carboxylation activity.

abstract: Fluids such as steam, oils, and molten salts are commonly used to store and transfer heat in a concentrating solar power (CSP) system. Metal oxide materials have received increasing attention for their reversible reduction-oxidation (redox) reaction that permits receiving, storing, and releasing energy through sensible and chemical potential. This study investigates the performance of a 111.7 MWe CSP system coupled with a thermochemical energy storage system (TCES) that uses a redox active metal oxide acting as the heat transfer fluid. A one-dimensional thermodynamic model is introduced for the novel CSP system design, with detailed designs of the underlying nine components developed from first principles and empirical data of the heat transfer media. The model is used to (a) size components, (b) examine intraday operational behaviors of the system against varying solar insolation, (c) calculate annual productivity and performance characteristics over a simulated year, and (d) evaluate factors that affect system performance using sensitivity analysis. Time series simulations use hourly direct normal irradiance (DNI) data for Barstow, California, USA. The nominal system design uses a solar multiple of 1.8 with a storage capacity of six hours for off-sun power generation. The mass of particles to achieve six hours of storage weighs 5,140 metric tonnes. Capacity factor increases by 3.55% for an increase in storage capacity to eight hours which requires an increase in storage volume by 33% or 737 m3, or plant design can be improved by decreasing solar multiple to 1.6 to increase the ratio of annual capacity factor to solar multiple. The solar reduction receiver is the focal point for the concentrated solar energy for inducing an endothermic reaction in the particles under low partial pressure of oxygen, and the reoxidation reactor induces the opposite exothermic reaction by mixing the particles with air to power an air Brayton engine. Stream flow data indicate the solar receiver experiences the largest thermal loss of any component, excluding the solar field. Design and sensitivity analysis of thermal insulation layers for the solar receiver show that additional RSLE-57 insulation material achieves the greatest increase in energetic efficiency of the five materials investigated. / Dissertation/Thesis / Masters Thesis Civil and Environmental Engineering 2017

abstract: Concentrating Solar Power (CSP) plant technology can produce reliable and dispatchable electric power from an intermittent solar resource. Recent advances in thermochemical energy storage (TCES) can offer further improvements to increase off-sun operating hours, improve system efficiency, and the reduce cost of delivered electricity. This work describes a 111.7 MWe CSP plant with TCES using a mixed ionic-electronic conducting metal oxide, CAM28, as both the heat transfer and thermal energy storage media. Turbine inlet temperatures reach 1200 °C in the combined cycle power block. A techno-economic model of the CSP system is developed to evaluate design considerations to meet targets for low-cost and renewable power with 6-14 hours of dispatchable storage for off-sun power generation. Hourly solar insolation data is used for Barstow, California, USA. Baseline design parameters include a 6-hour storage capacity and a 1.8 solar multiple. Sensitivity analyses are performed to evaluate the effect of engineering parameters on total installed cost, generation capacity, and levelized cost of electricity (LCOE). Calculated results indicate a full-scale 111.7 MWe system at $274 million in installed cost can generate 507 GWh per year at a levelized cost of $0.071 per kWh. Expected improvements to design, performance, and costs illustrate options to reduce energy costs to less than $0.06 per kWh. / Dissertation/Thesis / Masters Thesis Civil, Environmental and Sustainable Engineering 2017