A área incerto-hipotalâmica (IHy) é uma região diencefálica que atua em funções reprodutivas através da presença do hormônio concentrador de melanina (MCH). Nosso objetivo é estudar as conexões da IHy de fêmeas com áreas relacionadas ao controle neuroendócrino reprodutivo, usando traçador anterógrado dextrana amina biotinilada (BDA) e retrógrado Fluorogold (FG). Nesse sentido, destacamos que a IHy, em fêmeas, conecta-se reciprocamente com diversos núcleos hipotalâmicos da região periventricular e pré-óptica, além das áreas hipotalâmica anterior, posterior e lateral. Nosso resultados também sugerem um dimorfismo sexual das projeções da IHy, já que a área pré-óptica medial, o núcleo anteroventral periventrciular e o núcleo arqueado são mais densamente inervadas pelas IHy em fêmeas do que em machos. / The incerto-hypothalamic area (IHy) is a diencephalic region poorly studied which is characterized by two non-colocalized neurochemical groups composed by the A13 dopaminergic group intermingled with predominantly GABAergic cells co-expressing melanin-concentrating hormone (MCH) and/or cocaine and amphetamine regulated transcript (CART). Functional studies suggest that IHy is involved in the neuroendocrine control of female reproduction in specific metabolic states mediated by MCH. In this way, our aim is to study the IHy connections in female rats related to neuroendocrine control of female reproduction using neuronal anterograde Biotin Dextran Amine (BDA) and retrograde Flurogold (FG) tracers. As a result, we found that IHy projections are the lateral septal nucleus, bed nucleus of stria terminalis, paraventricular thalamic nucleus, reuniens thalamic nucleus, medial preoptic area, medial and lateral preoptic nucleus, median preoptic nucleus, periventricular hypothalamic nucleus, anteroventral periventricular nucleus, anterior, posterior and lateral hypothalamic area and precommissural nucleus. The main afferents to the IHy, in females, are the lateral septal nucleus, median preoptic nucleus and paraventricular thalamic nucleus. These results suggest sexually dimorphic projections from the IHy, since IHy more densely innervates neuroendocrine regions in female than in male rats.

O hormônio concentrador de melanina (MCH) foi inicialmente identificado em peixes teleósteos por regular a mudança de coloração, agregando os grânulos de pigmentos nas células pigmentares, levando ao clareamento dos animais. Possui dois receptores conhecidos: MCHR1, encontrado em todas as espécies, e o MCHR2, no SNC de alguns mamíferos e em outros tecidos de teleósteos. Devido a sua ampla distribuição, diversos estudos tem investigado o papel do MCH como um peptídeo que exerce diversas funções fisiológicas: regulação da ingestão de alimentos em mamíferos e peixes, regulação da resposta ao stress e à ansiedade, comportamento, locomoção, reprodução, sono, memória, inflamação. O objetivo desse trabalho foi investigar a possível participação do MCH na ativação das vias de sinalização para oxido nítrico em células de eritroforoma de Carassius auratus (GEM-81). Adicionalmente, incluímos o hormônio estimulador de melanócitos - ?-MSH no estudo, pois este é antagônico ao MCH nas mais diversas funções. Demonstramos que em GEM-81 ocorre a expressão da isoforma iNOS sem o estimulo de lipopolissacarídeos; e que com o tratamento de MCH e Soro Fetal Bovino (SFB) reduzido a 0,5%, sua expressão foi aumentada com o decorrer do tempo, com o máximo alcançado com 180 min e 360 min. Na produção de nitrito foi observado um aumento muito discreto nos mesmos tempos. O α-MSH não apresentou nenhuma diferença de expressão de iNOS e na produção de nitrito. Nossos dados apontam a existência da via do óxido nítrico em células de eritroforoma de Carassius auratus e sugerem que o MCH possa induzir a produção de oxido nítrico pelo aumento de expressão da iNOS / The melanin -concentrating hormone (MCH) was first identified in teleost fish by regulating the color change, aggregating the pigment granules in pigment cells, leading to bleaching of animals. It has two known receptors: MCHR1, found in all species, and MCHR2, in the CNS of mammals and some other tissues of teleosts. Because of their widespread distribution, various studies have investigated the role of MCH as a peptide that exerts various physiological functions: regulation of food intake in mammals and fish, regulation of stress responses and anxiety, behavior, locomotion, reproduction, sleep, memory and inflammation. The aim of this study was to investigate the possible involvement of MCH in the activation of signaling pathways for nitric oxide in cells of Carassius auratus eritroforoma (GEM -81). Additionally, we include the melanocyte stimulating hormone - α - MSH in the study, as this is antagonistic to MCH in various functions. We demonstrated that GEM -81 isoform expression of iNOS without lipopolysaccharide stimulation occurs, and the treatment with MCH and Fetal Bovine Serum (FBS) reduced to 0.5% increased the expression with the passage of time, the maximum reached at 180 min and 360 min. Regarding nitrite production, it was observed a very modest increase at the same time. The α-MSH showed no difference in the expression of iNOS or production of nitrite. Our data indicate the presence of the nitric oxide pathway in cells of eritroforoma Carassius auratus, suggesting that MCH could induce nitric oxide production by increasing the expression of iNOS .

The amount of incoming solar energy to earth is greater than any other source. Among existing technologies to harness solar energy there is concentrating solar power (CSP). One advantage of CSP is that is dispatchable, meaning that it can provide power even when the sun is not shining. However, CSP is undergoing challenges which hinder its development such as operating variabilities caused by the fluctuations of the sun or the fact that these systems are not yet cost competitive with respect to other technologies.   One way of improving the performance of CSP plants (CSPPs) is by increasing their operational flexibility, specifically their capability for fast starts. In this way it is possible for the CSPP to harness the solar energy as soon as possible, thus producing more energy and increasing its profitability. Over 90% of CSPPs use a steam turbine to generate electricity. Steam turbines are not currently designed with the flexibility required by the CSP application. Steam turbine start-up is limited by thermal stress and differential expansion. If not carefully controlled, these phenomena either consume lifetime or even result in machine failure. The aim of this work was to understand the improvement potential of steam turbine start-up and quantify this in terms of CSPP performance indicators. For this, a thermo-mechanical steam turbine model was developed and validated. The model was then used to analyze potential improvements and thermal constraints to steam turbine start-up operation. Furthermore, a CSP plant techno-economic model was developed including steam turbine details. This modeling approach including two levels of detail allowed for the particularities of the component to be included within the dynamics of the plant and thus be able to connect the perspectives of the equipment manufacturer with those of the plant operator. Reductions of up to 11.4% in the cost of electricity were found in the studies carried out. / <p>QC 20170814</p>

The following Master Thesis will talk about a C-PV prototype using bifacial PV technology, based on the Solarus Collector. The Solarus Collector consists in two PV cells built on a metallic receiver, where there are some water channels flowing through it, allowing to cool down the PV cells, thus increasing their efficiency. The collector also presents a reflector to provide irradiance to the back part of the receiver, where the other PV cells are located. The new prototype will present bifacial PV cells but not a metallic receiver. This construction aims to reduce the price of the receiver, but will not have a system to cool down the solar cells. This Master Thesis will be developed in the Solarus facilities, in collaboration with the Solarus members. In order to grasp an idea of this prototype, two main procedures will be done. Regarding the bifacial technology, a bifacial PV module will be measured under different conditions, depending on which sides can be illuminated or shaded. On the other hand, a thermodynamic simulation will be carried out on different geometries of the reflector and receiver, in order to figure out the evolution of the temperatures on the new prototype. This simulation will be done with a finite element method, widely known in this applications. The results will show several problems concerning this prototype. Although the measurements of the bifacial PV module will result beneficial and informative, the problem with the temperature will tend to back down this prototype. The lack of some system to cool down the bifacial cells will imply that the receiver could reach unacceptable temperatures. This hypothesis will be drawn under some specific conditions, so they will not be completely devastating to the idea of using bifacial cells, but perhaps a different approach should be used in case it is desired to continue this work.

Concern over the altering climate due to the release of anthropogenic greenhouse gases has caused a major shift in the developments of ways to minimise human impact on the climate. Solar energy is seen as one of the most promising sources to transform the energy market for low-carbon energy generation. Currently, solar power is generated via photovoltaic (PV) and concentrating solar power (CSP) technologies. The advantage of CSPs to scale up renewable energy to utility level, as well as to store thermal energy for electrical power generation when the sun is not available (after sunset or during cloudy periods) makes this technology an attractive option for sustainable clean energy. CSP development, however, is still in its infancy, and for it to be a competitive form of energy-generation technology, techno-economic developments in this field need to improve the efficiency and decrease the costs of this technology. A policy report by the European Academies’ Science Advisory Council (EASAC) (2011) indicated that central receiver (solar tower) CSP systems show the greatest margin for technological improvements (40% to 65% is estimated), and that an improvement in receiver technology could make the greatest contribution to increase efficiency. This study therefore focused on analysing the optical and thermal performance of a new proposed solar cavity molten salt receiver design for a central receiver CSP system using a numerical approach. In this study, the receiver’s performance was analysed by first selecting an existing heliostat field, Planta Solar 10 (PS-10). For the numerical analysis to reflect conditions that are as realistic as possible, numerical models for different aspects were selected and validated. For modelling the sun, the solar tracking numerical model proposed by Iqbal (1983) was selected and implemented after literature and comparison showed adequate results. The direct normal irradiation (DNI) was modelled by applying a clear sky model, with the parameterisation model C proposed by Iqbal (1983) as the chosen model. The variables in this model that were subject to temperature, and humidity values were more accurately presented by adding numerical approximations of the region’s actual weather data. The DNI model reflected realistic fluctuations. For the thermal modelling, a validation study was conducted on impingement flow heat transfer to select an appropriate Reynolds-averaged Navier-Stokes (RANS) model that would provide accurate results when conducting the thermal performance test on the receiver. The study concluded that the transitional Shear Stress Transport (SST) turbulence model performed the best. A new method was also developed and validated that allows one to not only simulate complex geometries within the Monte Carlo ray tracing environment SolTrace, but also to apply the results obtained by simulating this model as a heat source within the computational fluid dynamics (CFD) environment ANSYS Fluent. This allows SolTrace modelling to be more accurate, since models do not need to be approximated to simple geometries. It also provides an alternative for solar modelling in ANSYS Fluent. The optical analysis was conducted by first performing an analysis on the receiver aperture and studying its sensitivity on the captured flux. This was followed by analysing the optics of the proposed receiver, the flux distributions on a simplified absorber surface area, and how these distributions are altered by changing some parameters. An in-depth analysis was finally done on the absorber area by applying the aforementioned model to simulate complex geometries within SolTrace, with the results illustrating the difference of the detailed geometry on optical modelling. An alternative receiver design with improved optical features was proposed, with an initial study providing promising results. The thermal analysis was done within the CFD environment, with only a section of the absorber surface area considered, and by applying the solar flux simulated during the optical analysis as heat source within the geometry model. This allowed the model to simulate the effects of re-radiation at the surface of the absorber while simulating the heat transfer at the fluid molten salt side simultaneously. The results showed that, for the current design and requirements, the absorber surface temperature reaches impractical temperatures. Altering the design or being more lenient on the requirements has, however, shown dramatic improvements in terms of thermal performance. Sensitivity studies for both the optical and thermal analyses have shown that changes in design can dramatically improve the performance of the design, making it a possible feasible receiver design for central receiver systems. / Dissertation (MEng)--University of Pretoria, 2019. / National Research Foundation (NRF) / Mechanical and Aeronautical Engineering / MEng / Unrestricted

The way its future power systems are designed will have significant impact on sub-Saharan Africa's (SSA) aspirations to move from low electricity consumption rates to enhance life quality and further increase economic opportunity. At present, Africa is experiencing higher economic growth rates than other continents (including Asia). And so is its need for electric power. However, all too often the options that are chosen are the ones with lowest risk and that require little coordination. In part, this is because region-wide planning, coordination and institutions are in their infancy. “Low risk” power plants typically include oil generators that can be sited close to loads, other fossil fuel power plants, and hydro plants that can easily be connected to the continent’s grid. However, hydropower production has been limited due to changes in weather and climate and socio-economic impacts. Additionally, its potential has also not been reached as large sites are far from adequate grids. A restructuring of the energy system that considers both the potential for increased geographical integration while moving gradually towards more sustainable electricity generation may hold significant promise. This work considers the potential of another renewable technology namely concentrating solar power (CSP) and connecting supply and demand centers via high voltage direct current (HVDC) power lines. Specifically, the focus is on utility-scale solar power generation to supply the needs of growing urban centers of demand. It develops a Geographic Information System-based (GIS) model with a spatial resolution of 30 arc-seconds to calculate the cost evolution of the electricity produced by different technologies of CSP plants and the costs of grid development to selected centers of demand. The results show that major SSA metropolis can benefit from distant CSP economically attractive to compete with inlaid coal-based generation. In 2010, total imports of coal exceeded 1.4 million short tons with consequent economic and environmental costs. Solar towers plants endowed with thermal storage may become a leading technology for smoothing purposes with zero fuel costs. Furthermore, Africa’s vast solar resources are far from urban centers of demand and a transmission system capable to integrate high levels of renewable energy while improving reliability of supply is required. The results of this study point to the importance of SSA centers to rely on a Super Grid approach to take advantage from CSP least-cost potential and to discontinue expensive traditional sources. Overall, solar corridors can integrate with geographically-wide wind and hydro potentials to create clean energy corridors and encourage a transition towards more sustainable energy systems.

Gammaproteobacterium Thiomicrospira crunogena thrives at deep-sea vents despite extreme oscillations in the environmental supply of dissolved inorganic carbon (DIC; =CO2 + HCO3- + CO3-2). Survival in this habitat is likely aided by the presence of a carbon concentrating mechanism (CCM). Though CCMs are well-documented in cyanobacteria, based on this study T. crunogena is the first chemolithoautotroph to have a physiologically characterized CCM. T. crunogena is capable of rapid growth in the presence of 20 micrometers DIC, has the ability to use both extracellular HCO3- and CO2, and generates intracellular DIC concentrations 100-fold greater than extracellular, all of which are consistent with a CCM analogous to those present in cyanobacteria. Interestingly, however, the T.crunogena genome lacks apparent orthologs of many of the components of the cyanobacteria CCM (e.g., HCO3- transporters). However, despite this lack, several candidate genes were identified during genome annotation as likely to play a role in DIC uptake and fixation (three carbonic anhydrase genes: alpha-CA, beta-CA, and csoSCA, as well as genes encoding three RubisCO enzymes: cbbLS, CScbbLS, and cbbM, which encode a cytoplasmic form I RubisCO, a carboxysomal form I RubisCO, and a form II RubisCO, respectively). In order to clarify their possible roles in DIC uptake and fixation, alpha-CA, beta-CA and csoSCA transcription by low-DIC and high-DIC T. crunogena were assayed by qRT PCR, heterologous expression in E. coli, and potentiometric assays of low-DIC and high-DIC T. crunogena. Transcription of alpha-CA and beta-CA were not sensitive to the DIC concentration available during growth. When overexpressed in E.coli, carbonic anhydrase activity was detectable, and it was possible to measure the effects of the classical carbonic anhydrase inhibitors ethoxyzolamide and acetazolamide, as well as dithiothreitol (DTT; recently determined to be a carboxysomal CA inhibitor). The alpha-CA was sensitive to both of the classical inhibitors, but not DTT. Beta-CA was insensitive to all inhibitors tested, and the carboxysomal carbonic anhydrase was sensitive to both ethoxyzolamide and DTT. The observation that the CA activity measureable potentiometrically with intact T. crunogena cells is sensitive to classical inhibitors, but not DTT, strongly suggests the alpha-CA is extracellular. The presence of carbonic anhydrase activity in crude extracts of high-DIC cells that was resistant to classical inhibitors suggests that beta-CA may be more active in high-DIC cells. Incubating cells with ethoxyzolamide (which permeates cells rapidly) resulted in inhibition of carbon fixation, but not DIC uptake, while incubation with acetazolamide (which does not permeate cells rapidly) had no apparent effect on either carbon fixation or DIC uptake. The observations that inhibition of alpha-CA has no effect on DIC uptake and fixation, and that the beta-CA is not transcribed more frequently under low-DIC conditions, make it unlikely that either play a role in DIC uptake and fixation in low-DIC cells. Further studies are underway to determine the roles of alpha-CA and beta-CA in T. crunogena. To assay the entire genome for genes transcribed more frequently under low-DIC conditions, and therefore likely to play a role in the T. crunogena CCM, oligonucleotide arrays were fabricated using the T. crunogena genome sequence. RNA was isolated from cultures grown in the presence of both high (50 mM) and low (0.05 mM) concentrations of DIC, directly labeled with cy5 fluorophore, and hybridized to microarrays. Genes encoding the three RubisCO enzymes present in this organism demonstrated differential patterns of transcription consistent with what had been observed previously in Hydrogenovibrio marinus. Genes encoding two conserved hypothetical proteins were also found to be transcribed more frequently under low-DIC conditions, and this transcription pattern was verified by qRT-PCR. Knockout mutants are currently being generated to determine whether either gene is necessary for growth under low-DIC conditions. Identifying CCM genes and function in autotrophs beyond cyanobacteria will serve as a window into the physiology required to flourish in microbiallydominated ecosystems where noncyanobacterial primary producers dominate.

Kuba har i dagsläget ett högt beroende av importerad olja, för att tillgodose sin växande efterfrågan på elektricitet. Importen sker främst från Venezuela. Detta beroende gör Kuba känsligt för ändringar i oljepriser samt det politiska klimatet. Den nuvarande krisen i Venezuela har haft en betydande inverkan på Kubas elproduktion. Genom att utöka landets förnybara energikällor kan Kuba minska sitt beroende av andra länder och diversifiera sin energiförsörjning. Detta kommer även att leda till en positiv miljöpåverkan då landets CO 2-utsläpp minskar. Kubas geografiska läge har ideala förhållanden för förnyelsebar energigenerering, så som solkraft. Solkraft utvecklas konstant och innehåller en hög potential. Concentrating Solar Power (CSP) är en teknologi där speglar och/eller linser används för att koncentrera solljus till en liten yta som konverterar solljuset till värme. Denna värme kan sedan användas i termodynamiska cykler. Det finns två huvudsakliga problem med implementering av CSP på Kuba. För det första är CSP beroende av momentana väderförhållanden, vilket leder till en oregelbunden elproduktion. För det andra har CSP höga investeringskostnader. För att adressera dessa problem, är det möjligt att implementera CSP i ett redan existerande kraftverk med regelbunden energikälla, d.v.s. skapa ett hybridkraftverk. På så vis uppnås regelbunden elproduktion med signifikant lägre investeringskostnad. Ett sådant existerande kraftverk kan hittas hos många av Kubas sockerbruk. Den här studien undersöker möjligheten att implementera solkraft i sockerbruket Carlos Baliño, beläget i Villa Clara, Kuba. Fabriken är självförsörjande av elektricitet på årlig basis. De använder en Rankine-cykel för att generera el och processvärme som används i sockerframställningen. Bränslet som används är bagasse, en restprodukt efter att sockerjuicen pressats ut ur sockerrören. Fyra CSP-teknologier och tre implementeringslayouts undersöktes, vilket resulterade i att parabolic trough-teknologin och förvärmning av vatten ansågs vara de bästa alternativen för Kuba och Carlos Baliño. Vidare undersöktes två olika scenarier för CSP. Scenario 1 innefattar implementering av CSP i sockerbruket under rådande skick och Scenario 2 består av implementering av CSP efter en investering gjorts i en Condensing Extraction turbin (CEST). Resultatet visar att Carlos Baliño bör investera i CEST innan de implementerar CSP, det vill säga Scenario 2. Detta beror på att i scenario 1 är det inte möjligt att generera elektricitet utanför sockersäsongen, vilket leder till att en stor del av solpotentialen inte kan utnyttjas. Den maximala investeringskostnaden för scenario 1 är 3,7 MUSD, vilket inte är en realistisk kostnad. Den maximala investeringskostnaden för scenario 2 beror av tillgänglig bagasseimport och är 5,9 – 7,2 MUSD. Att investera i CSP rekommenderas ej om bagasseimporten är obegränsad. Givet att bagasseimporten är begränsad skulle CSP-implementeringen leda till en utökad elproduktion av 5,4 – 7,2 GWh/år, en årlig minskning av oljeanvändandet med 16 100 – 21 800 tunnor och minskade CO2-utsläpp med 12 00-16 00 ton årligen. Carlos Baliños ekonomiska resultat skulle öka med 0,5 MUSD/år och den kubanska statens med 0,7 – 0,9 MUSD/år. Framtida studier rekommenderas undersöka möjligheten till generering av el året runt vid Carlos Baliño utan en CEST, solkraftsefterfrågan på nationell nivå och potentiella utvecklingar av solkraft hos Carlos Baliño. / Cuba is currently highly dependent on imported oil, mainly from Venezuela, to meet their growing electricity demand. This dependence makes Cuba sensitive to changes in oil price as well as the political climate. The current crisis in Venezuela has a large impact on Cuba’s electricity generation. By expanding its renewable energy sources Cuba could decrease their dependence on other countries and diversify their energy supply. Moreover, it would have a positive climate impact by reducing the country’s CO2-emissions. Geographically, Cuba has ideal conditions for renewable energy utilization, such as solar power. Solar energy is constantly progressing and is considered a great source of energy. Concentrating Solar Power (CSP) is a technology which applies mirrors and/or lenses to concentrate the sunlight onto a small area which converts the sunlight into heat, possible to use in a thermodynamic cycle. There are mainly two problems with the implementation of CSP in Cuba. Firstly, CSP is a non-dispatchable power generating system since it is dependent on the instantaneous weather conditions. Secondly, it has high investment costs. One way of solving these problems is by implementation CSP in an already existing power plants with a dispatchable source of energy, making it a hybrid power plant. Accordingly, the hybrid power plant would be dispatchable and the investment costs would be significantly lower. Existing power plants can be found in Cuban sugar mills. This study investigates the possibility to implement solar power in the sugar mill Carlos Baliño, located in Villa Clara, Cuba. The factory is currently self-sufficient electricity wise on a yearly basis, using a co-generation Rankine cycle to generate electricity and process heat used in the sugar production. The fuel used is bagasse, a rest product obtained after the sugar juice has been pressed out of the sugar canes. Four CSP-technologies and three implementation layouts were examined, resulting in the parabolic trough-technology and feedwater heating being considered the optimal solution. Furthermore, two different scenarios for CSP was investigated; implementation of CSP in the mill at the current state (scenario 1) or after investing in a Condensing-Extraction Turbine (CEST) (scenario 2). The results show that Carlos Baliño should invest in a CEST before considering implementation of CSP. Off-season operation is not available for scenario 1, leading to a vast amount of solar potential being unexploited. The maximal investment allowed for scenario 1 is 3.7 MUSD, which is not a realistic number. The maximal investment allowed for in scenario 2 is 5.9 – 7.2 MUSD, depending on bagasse import availability. If bagasse import is unlimited, it is not recommended to invest in solar power. Implementation of CSP in scenario 2 regarding bagasse import limits would yearly lead to an additional electricity generation at Carlos Baliño of 5.4 – 7.3 GWh, decrease the oil usage with 16,100 – 21,800 barrels and the CO2-emissons with 1,200 – 1,600 tonnes. Carlos Baliño’s annual yield would increase with 0.5 – 0.6 MUSD/year and the Cuban states annual yield would increase with 0.7 – 0.9 MUSD/year. Future work is recommended to explore alternatives to all year-around electricity generation in Carlos Baliño without investing in a CEST, investigate solar power demand on a national level, and examine possible developments of the suggested solar field, for instance solar-only operation.

No description available.

Les neurones exprimant l'hormone de mélano-concentration (MCH) sont observés dans l'hypothalamus postérieur de tous les vertébrés, de la lamproie à l'Homme. Ces neurones sont impliqués dans diverses fonctions comme le cycle veille/sommeil ou la prise alimentaire. Ils forment une population non homogène et au moins deux sous-populations sont reconnues, chez le rat. La première sous-population est composée de neurones nés au 11ème jour de vie embryonnaire (E11) qui projettent massivement sur les régions les plus postérieures du système nerveux central. La seconde est générée à E12/E13 et les neurones la caractérisant projettent sur les régions les plus antérieures du cerveau et expriment le peptide CART (cocaine and amphetamine regulated transcript) et le récepteur NK3 (neurokinine). L'objectif de notre travail était de comprendre l'origine de ces deux sous-populations. Pour cela, nous avons utilisé des approches histologiques, moléculaires et in vitro. Les neurones à MCH sont parmi les premiers neurones à naître et à différencier leur phénotype chimique le long d'une région longitudinale définie par une prolifération intense, appelée " cell cords " par Keyser en 1972. Cette bande longitudinale est caractérisée par l'expression de gènes comme Sonic Hedgehog (Shh), Nkx2.1, Nkx2.2 et a été récemment renommée " diagonale intrahypothalamica " ou ID. La différenciation des neurones à MCH dépend de l'expression du facteur morphogène Shh et ces neurones expriment Nkx2.1 et Nkx2.2, facteurs de transcription régulés positivement par Shh. Les neurones de la première sous-population envoient des projections le long du premier tractus longitudinal à se mettre en place, le tractus postopticus (tpoc). Ceux issus de la deuxième sous-population se différencient concomitamment au développement des régions télencéphaliques et leurs projections changent de direction pour innerver les régions antérieures du cerveau sous la dépendance de protéines de guidage axonal, Nétrine1 et Slit2. Nétrine1 permet d'attirer les axones MCH exprimant le récepteur DCC précocement vers la moelle épinière et plus tardivement vers le télencéphale alors que Slit2 contraint les axones MCH exprimant Robo2 à sortir de l'hypothalamus. L'étude du modèle " MCH " permet de mettre en lumière un changement d'organisation précocement au cours du développement dans l'axe longitudinal du prosencéphale. La bande longitudinale d'expression des facteurs de transcription Shh, Nkx2.2 peut être perçue comme une extension rostrale de la colonne neurogénique médiane déjà décrite chez des espèces d'invertébrés possédant une symétrie bilatérale. Les neurones générés le long de cette colonne le sont très tôt au cours du développement. / Neurons expressing melanin-concentrating hormone (MCH) are observed in the vertebrate posterior hypothalamus, from lampreys to humans. These neurons are involved in various functions such as sleep/wake cycle or food intake. They form a non-homogeneous population and at least two sub-populations are indentified in the rat. The first sub-population is composed of neurons born on the 11th embryonic day (E11) that project heavily on posterior regions of the central nervous system. The second is characterized by neurons born at E12/E13, projecting in anterior regions of the brain and expressing the peptide CART (cocaine and amphetamine Regulated Transcript) and the NK 3 receptor (neurokinin). The aim of this study was to understand the origin of these two sub-populations. For this, we used histological, molecular and in vitro approaches. MCH neurons are among the first neurons to be born and to differentiate their chemical phenotype along a longitudinal region defined by intense proliferation and called " cell cord " by Keyser in 1972. This longitudinal band is characterized by the expression of genes such as Sonic Hedgehog (Shh), Nkx2.1, Nkx2.2 and was recently named " diagonal intrahypothalamica " or ID. Differenciation of MCH neurons depends on expression of the morphogenetic factor Shh and these neurons express Nkx2.1 and Nkx2.2, transcription factors upregulated by Shh. The neurons of the first sub-population send projections along the tractus postopticus (tpoc), which is the first longitudinal tract to develop. Neurons of the second sub-population differentiate concomitantly to the development of the basal forebrain and their projections innervate anterior brain regions. Our results obtained in vitro showed that Netrin1 attracts MCH axons and that this reponse is mediated by DCC. Slit2 repulses MCH axons and this reponse is mediated by the Robo2 receptor. Overall, our study of the development of the MCH system shed light on an organizational change in the longitudinal axis of the forebrain during early development : a primary longitudinal organization characterized by the longitudinal expression of Shh and Nkx2.2 and the path of the tractus postopticus in the diencephalon and mesencephalon. MCH neurons of the first sub-population develop during this stage. Then, as the basal telencephalon extends and expresses Netrin1, the medial forebrain bundle differentiates, inducing a change in the main axis of the forebrain ; meanwhile MCH neurons of the second sub-population appear. MCH sub-populations reflect distinct developmental stages of the forebrain.