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Tissue Engineering a Blood Vessel Mimic While Monitoring Contamination Through Sterility Assurance TestingDjassemi, Navid 01 July 2012 (has links) (PDF)
Tissue Engineering A Blood Vessel Mimic While Monitoring Contamination Through Sterility Assurance Testing
Navid Djassemi
Tissue engineering blood vessel mimics has been proposed as a method to analyze the endothelial cell response to intravascular devices that are used in today’s clinical settings for the treatment of cardiovascular disease. Thus, the development of in vitro blood vessel mimics (BVMs) in Cal Poly’s Tissue Engineering Lab has introduced the possibility of assessing the characteristics of cellular response to past, present, and future intravascular devices that aim at treating coronary artery disease.
This thesis aimed at improving the methods and procedures utilized in the BVM model. Initial aspects of this project focused on using an expanded polytetrafluoroethylene (ePTFE) scaffold in conjunction with human endothelial cells to replicate the innermost intimal layer of a blood vessel. Human umbilical vein endothelial cells (HUVECs) were pressure sodded onto ePTFE scaffolds through cell sodding techniques in an attempt to effectively and consistently replicate and assess the intimal layer. Through each study ePTFE grafts were subjected to different culture times and steady flow rates to observe and compare the differences in the endothelial cell deposition. Results were inconsistent, although moderate cell adhesion was noted throughout each of the BVM setups. Each study exhibited a range of cell sodding density rates.
In the second phase of the thesis, contamination assessment protocols were implemented in the BVM lab. The intent of this part of the project was to assess the relative sterility in the cell culture lab, a critical component involved with the success or hindrance of cell and tissue cultures. Using microbiological validated methods, microbiological tests were conducted to examine the levels of microbial growth in and around the tissue engineering lab.
Results were tracked over a two month period in the lab with several observations of aerobic microorganism growth on various counter and lab surfaces. Higher growth trends were found on surfaces outside the cell culture lab, in the general TE lab area. These findings were used to provide overall suggestions on tracking microbes for long-term durations in ongoing BVM setups to directly improve the overall sterility assurance of the studies.
As the project reached its conclusion a look back at all the BVM setups and contamination assessments lead to a few suggestions for improving aseptic techniques within the TE lab, such as monitoring microbial growth in the culture processes, creating limit specifications, and creating a standardized way to regulate quality control within the lab environment. Furthermore, as the development BVM evolves, the findings from this report can be used with related research for improving the culture conditions of various BVM studies.
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Vulnerabilidade intrínseca e risco de contaminação do aquífero livre da bacia hidrográfica do Rio Gramame-PBLinhares, Franklin Mendonça 18 December 2012 (has links)
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Previous issue date: 2012-12-18 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The vulnerability mapping and risk analysis of groundwater contamination are essential for guiding management plans of watersheds. Currently, new criteria for eligibility of groundwater are required by CONAMA Resolution No. 396/2008 and justifies this study. The chosen basin for mapping of vulnerability of a free aquifer was the Gramame river basin in Paraíba State. To build the vulnerability map the DRASTIC method was chosen, acronym of factors to be analyzed: Depth to the water table, net Recharge, Aquifer media, Soil media, Topography, Impact of the unsaturate zone, Hydraulic Condutivity. For the pollution risk mapping the modified DRASTIC was used, where the characteristics of land use and land cover are also considered. Each DRASTIC factor is built dynamically as a layer of a Geographic Information System (GIS). Environment features of the system are specialized and, in the final step, are integrated in order to generate vulnerability map. The preliminary risk analysis is made by overlapping the vulnerability map to the land cover and land use maps. Data from static levels of 27 shallow wells were collected monthly during a hydrological year (2010/2011), which allowed to divide them into two periods: wet and dry. With this determination it was possible to compare the vulnerability maps for these two distinct periods. It was found in this study that, in the rainy season, the vulnerability is greater than in the dry season. The result of the intrinsic vulnerability map in the rainy season showed that the central and southwest regions, where the city of Pedras de Fogo is located, presents high vulnerability. The risk of contamination map identified these same regions as very high risk due to human action. These data can be used to define and direct resources to areas where protection is needed, as well as subsidize the municipal and state management in making decisions regarding land use, actions development and measures to ensure the environmental quality of the groundwater of Gramame river basin. / O mapeamento de vulnerabilidade e a análise de risco de contaminação das águas subterrâneas são fundamentais para a orientação dos planos de gerenciamento de bacias hidrográficas. Atualmente, novos critérios de enquadramento das águas subterrâneas são exigidos pela Resolução do CONAMA Nº 396/2008 e justificam a o presente estudo. O mapeamento da vulnerabilidade do aquífero livre foi da realizado na bacia hidrográfica do rio Gramame. Para a construção do mapa de vulnerabilidade, foi utilizado o método DRASTIC, acrônimo dos fatores a serem analisados: Depth to the water table, Net Recharge, Aquifer media, Soil media, Topography, Impact of the unsaturate zone, Hydraulic Condutivity. Já para o mapeamento do risco à poluição foi utilizado o DRASTIC modificado, onde são consideradas (também) as características de uso e cobertura do solo. Cada fator do DRASTIC é construído de forma dinâmica, como uma camada, no Sistema de Informação Geográfica (SIG) a partir de características do meio que são especializadas e, na fase final, integrada para gerar o mapa de vulnerabilidade. A análise preliminar de risco é feita pela sobreposição do mapa de vulnerabilidade como os mapas de cobertura do solo e uso do solo da bacia hidrográfica do rio gramame. Gerou-se com essas informações um banco de dados necessário à confecção dos mapas de vulnerabilidade intrínseca e de risco de contaminação. Os dados dos níveis estáticos dos 27 poços foram coletados mensalmente durante um ano hidrológico (2010/2011), o que permitiu dividi-los em dois períodos: chuvoso e estiagem. Com essa determinação foi possível realizar uma analise comparativa entre os mapas de vulnerabilidade para estes dois períodos distintos. Identificou-se nesta pesquisa que, no período chuvoso, a vulnerabilidade é maior que no período de estiagem. O resultado do mapa de vulnerabilidade intrínseca no período chuvoso revelou que a região central e sudoeste, onde está localizada a cidade de Pedras de Fogo, apresenta vulnerabilidade alta. O mapa de risco de contaminação identificou essas mesmas regiões como de áreas risco muito alto, devido à ação antrópica. Esses dados podem ser usados para delimitar e direcionar recursos para as áreas que necessitem de proteção, bem como subsidiar a gestão municipal e Estadual na tomada de decisões quanto ao uso do solo, elaboração de ações e medidas que assegurem a qualidade ambiental das águas subterrâneas da bacia hidrográfica do rio Gramame.
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Cross-Contamination Risk of Dental Tray Adhesives: An In Vitro StudyPaczkowski, Isabel, Stingu, Catalina S., Hahnel, Sebastian, Rauch, Angelika, Schierz, Oliver 05 May 2023 (has links)
Background: The aim of this study was to investigate the risk of cross-contamination in dental tray adhesives with reusable brush systems. Methods: Four dental tray adhesives with different disinfectant components were examined for risk as a potential transmission medium for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus oralis, and Candida albicans. Bacterial and fungal strains were mixed with artificial saliva. The contaminated saliva was intentionally added to tray adhesive liquid samples. At baseline and up to 60 min, 100 microliters of each sample were collected and cultivated aerobically on Columbia and Sabouraud agar for 24 or 48 h, respectively. Results: At baseline, contamination with Staphylococcus aureus and Candida albicans could be identified in three out of four adhesives. In the subsequent samples, low counts of up to 20 colony-forming units per milliliter could be observed for Staphylococcus aureus. All other strains did not form colonies at baseline or subsequently. Adhesives with isopropanol or ethyl acetate as disinfectant additives were most effective in preventing contamination, while adhesives with hydrogen chloride or acetone as a disinfectant additive were the least effective. Conclusion: Within 15 min, the tested adhesives appeared to be sufficiently bactericidal and fungicidal against all microorganisms tested.
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The microbiological safety of fresh produce in Lebanon : a holistic 'farm-to-fork chain' approach to evaluate food safety, compliance levels and underlying risk factorsFaour-Klingbeil, Dima January 2017 (has links)
The consumption of unsafe fresh vegetables has been linked to an increasing number of outbreaks of human infections. In Lebanon, although raw vegetables are major constituents of the national cuisine, studies on the safety of fresh produce are scant. This research employed a holistic approach to identify the different stages of the food chain that contribute to the microbiological risks on fresh produce and the spreading of hazards. A thorough analysis of the institutional and regulatory framework and the socio-political environment showed that the safety of local fresh produce in Lebanon is at risk due to largely unregulated practices and shortfalls in supporting the agricultural environment as influenced by the lack of a political commitment. Microbiological analysis showed that the faecal indicator levels ranged from < 0.7 to 7 log CFU/g (Escherichia coli), 1.69-8.16 log CFU/g (total coliforms) and followed a significantly increasing trend from fields to the post-harvest washing area. At washing areas, Salmonella was detected on lettuce (6.7% of raw vegetables from post-harvest washing areas). This suggested that post-harvest cross-contamination occurs predominantly in the washing stage. At retails, a combination of observation and self-reported data provided an effective tool in assessing knowledge, attitudes and practices. It showed that the food safety knowledge and sanitation practices of food handlers were inadequate, even among the better trained in corporate-managed SMEs. Overall, the microbiological quality of fresh-cut salad vegetables in SMEs was unsatisfactory. The link between Staphylococcus aureus and microorganism levels on fresh salads vegetables and the overall inspection scores could not be established. On the other hand, inspection ratings on individual components, e.g., cleanliness and cross-contamination preventive measures showed significant correlation with Listeria spp. levels. Together, results confirmed that inspection ratings don’t necessary reflect the microbiological safety of fresh vegetables and that the application of control points of risk factors that likely to contribute to microbial contamination in the production environment are essential. The washing methods were limited in their effectiveness to reduce the contamination of parsley with Salmonella. In general, the pre-wash chopping and storing of parsley at 30ºC reduced the decontamination effect of all solutions, including sodium dichloroisocyanurate which was reduced by 1.3 log CFU/g on both intact and chopped leaves stored at 30ºC. In such conditions, the transfer rate of Salmonella from one contaminated parsley to subsequently chopped clean batches on the same cutting board(CB) recorded 60%-64%. Furthermore, the transmission of Salmonella persisted via washed CBs stored at 30°C for 24 h. It is recommended to keep parsley leaves unchopped and stored at 5ºC until wash for an optimum decontamination effect and to apply vigilant sanitation of CBs after use with fresh produce. This research presented important data for quantitative risk assessment for Salmonella in parsley and useful descriptive information to inform decision-makers and educators on microbial hazards associated with fresh produce in Lebanon. It also highlighted the risks areas that require urgent interventions to improve food safety. Considering the complex institutional and political challenges in Lebanon, there is an obvious need to direct development programs and support towards local agriculture production, effective education strategies and growing awareness of consumers and stakeholders on food safety related risks.
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Évaluation des risques de contamination des eaux de surface sur des bassins versants agricoles. Approches multiscalaires par modélisation spatiale et analyse multicritère pour l'aide à la décision / Assessing the risk of surface water contamination in agricultural watersheds. Multiscale approaches using spatial modelling and multi-criteria analysis for decision supportMacary, Francis 25 June 2013 (has links)
Il est désormais avéré que les pratiques intensives de production agricole mises en œuvre depuis les années 1960 en Europe, sont responsables de la dégradation des écosystèmes et notamment de la qualité des eaux de surface et souterraines. Afin de respecter les objectifs de résultat définis par la Directive Cadre sur l’Eau à l’échéance 2015, les gestionnaires de la ressource ont désormais ciblés prioritairement la protection des zones d’alimentation des captages. Cela nécessite une évaluation environnementale à une échelle adaptée aux mesures mises en œuvre. Ces éléments expliquent les objectifs de cette thèse, à savoir la contribution au développement de méthodes d’évaluation environnementale des activités agricoles à l’échelle des territoires, en complément de celles déjà existantes. Nous avons ainsi utilisé (i) des méthodes d’analyse et de modélisation multicritère (ELECTRE) combinées avec un système d’information géographique au niveau de petits bassins versants élémentaires de 2 à 5 km2 (sur le bassin de l’Oir, en Basse Normandie, Nord-ouest de la France et celui d’Auradé sur les Coteaux de Gascogne, Sud-ouest de la France) (ii) une approche par modélisation spatiale (dénommée PIXAL) des risques agro-environnementaux en utilisant la télédétection et un SIG, au niveau d'un grand espace à enjeu environnemental de plusieurs milliers de km2, (Coteaux de Gascogne). Dans les deux approches, nous avons montré que la combinaison de critères de vulnérabilité des eaux et de pression agricole choisis de façon pertinente, permettent d’évaluer les risques agro-environnementaux à différents niveaux d’organisation spatiale. L'évaluation à la parcelle agricole (niveau de la prise de décision par les acteurs de terrain) procure une représentation au niveau du bassin versant élémentaire. L'évaluation au pixel de l’image satellitale (Landsat 5-TM) permet une représentation à différents niveaux de bassins versants emboîtés par agrégation. En perspective, un couplage entre un modèle d'analyse multicritère avec un modèle spatial, pourrait permettre d’améliorer la performance de l’évaluation aux différents niveaux de l’action publique ou de terrain et d’apporter des éléments de réponse supplémentaires à la problématique du changement d’échelle spatiale. / There is evidence that intensive farming practices applied in Europe since the 1960s are responsible for the degradation of certain ecosystems, and a reduction in the quality of surface and ground water. Excessive use of nitrogen-based fertilisers and pesticides have led to high concentrations of these substances being found in rivers, thus compromising raw drinking water sources. Soil erosion – caused by simplified crop rotations, feeding livestock on corn instead of grass, and the removal of elements such as embankments, trees, hedges, etc. – has been conducive to the transfer of these particles into surface water. The resulting turbidity has led to the clogging up of spawning grounds, with many negative effects on their biological quality. Complementing existing approaches, this thesis will focus on the development of methods for carrying out territorial environmental analysis of farming activities. For our study, we used (i) a multicriteria analysis and modelling (ELECTRE) combined with a Geographic Information System (GIS) on a selection of small elementary watersheds (ranging in size from 2 km² to 5 km²). These were located in the Oir basin, in Lower Normandy Region, in northwest France, and the Auradé basin, in the hills of Gascony region, southwest. We also employed (ii) a spatial modelling approach (PIXAL) to represent agri-environmental risks. Applied to an environmentally-sensitive area (hills of Gascony region) of several thousand square kilometres, the PIXAL method involved evaluating agri-environmental risks through a combination of remote sensing and GIS. In both cases, we showed that choosing the most relevant criteria allows agri-environmental risks to be assessed at different organisational scales. Analysis at farm plot level (where decisions are made by stakeholders in the field) gives a representation of the elementary watershed. Pixel-based evaluation, using satellite imaging (Landsat TM5), provides a nested multi-aggregation view of different watersheds. Placing this study in perspective, coupling ELECTRE-style multicriteria analysis with a spatial model could be an effective way to improve analysis at different policy-making and territorial scales, as well as providing additional support where assessment needs to switch between those varying scales.
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Stanovení těžkých kovů v půdách a sedimentech v oblasti Revúce / Determination of heavy metals in soils and sediments in the region RevúceKováč, Lukáš January 2011 (has links)
This thesis aims to assess the extent of contamination of soil and sediment with risk metals in Jelšava. The main polluter of the environment in this area is the magnetize factory. Sampling of soils and sediments were made in three seasons. Sampling times were staggered to represent different seasons and to take into account the impact of the seasons on the obtained values of the concentrations in soil and sediment. Sampling sites were selected according according to the different distances from the factory and according to the different loading of roads. In each of the collected samples were determined concentrations of the elements copper, lead, zinc, nickel, manganese, mercury, cadmium and vanadium. Copper, lead, zinc, nickel and manganese were analyzed by atomic absorption spectrometry with atomization in a flame (F AAS), cadmium and vanadium were determined by atomic absorption spectrometry with electrothermal atomization (ET AAS). For the determination of mercury was used mercury analyzer (AMA).
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Combining measurements, remote sensing and numerical modelling to assess multi-scale flow dynamics in groundwater-dependent environmental systemsNixdorf, Erik 04 June 2018 (has links) (PDF)
Groundwater flow modelling provides an important quantitative instrument for addressing issues related to the quantity and quality of groundwater and the connected water resources. Consequently, groundwater flow models have been developed and used ubiquitously in science to deepen the understanding of subsurface processes and their drivers as well as management and planning tools.
The present work investigates how numerical models can be linked to field investigations and public databases to quantitatively approach questions in the area of groundwater research. The primary goal is to develop new, efficient ways to overcome limitations of the individual hydrological concepts for solving specific hydrological problems and to increase the understanding of practical applicability of different methods. For this purpose, tailor-made approaches were developed for different study areas covering diverse spatial scales: the hydrology of a small mining lake, the riparian aquifer at the scale of a single meander as well as the aquifer systems of a large-scale river basin in China.
The first part of the work deals with the physical and mathematical modelling of water constituents balance in a meromictic mining lake in Lusatia. The capability of using a rather simple mass-balance model based on a sufficient dataset of field data to evaluate lake stratification and lake-groundwater interaction were shown.
In the second part, a transient numerical groundwater flow model was developed for the riparian aquifer of a stream meander and was calibrated by three different salt tracer tests. The model was used to proof the reliability of subsurface travel times derived from time series analysis and to give insights in the riparian zone dynamics during changing hydraulic gradients.
The third part of the work describes the methodology to conduct risk assessment of groundwater contamination on the large catchment scale of the Songhua River in China. A comprehensive literature study was conducted to get an overview about measurement data on water quality data in China. A three-dimensional numerical flow and mass transport model was applied to access the flow and matter transport dynamics in the aquifer system of a sub-basin considering changing groundwater exploitation scenarios. Consequently, numerical groundwater modelling was combined with processed remote sensing and web mapping service data to overcome field data limitations and to derive groundwater vulnerability, groundwater hazard and groundwater risk maps for the entire Songhua River Basin.
Summarizing, this doctoral thesis could develop new methods of combining field measurements, data assimilation and aggregation from various sources and groundwater modelling strategies and successfully apply these methods to find solutions on problems of multiple scales and across water systems. / Die Grundwassermodellierung stellt eine wichtige wissenschaftliche Methode zur quantitativen Analyse von Fragestellungen zum Schutz der Menge und Güte der Grundwasserressourcen sowie der angeschlossenen Wasserkörper dar. Dementsprechend werden Grundwassermodelle sowohl für Planungs- und Bewertungszwecke im Wasserressourcenmanagement als auch zur wissenschaftlichen Erforschung der Prozesse im Untergrund entwickelt und angewendet.
Die vorliegende Arbeit untersucht in diesem Rahmen, wie numerische Modelle, Feldmessungen und Daten generiert aus Fernerkundungsdaten und Webplattformen systematisch verknüpft werden können, um Fragestellungen im Bereich der Grundwasserforschung quantitativ zu beantworten. Das Ziel der Arbeit ist es neue effiziente Abläufe zu entwickeln, die die Limitierung der einzelnen Methoden überwinden und diese auf deren Anwendbarkeit für die Lösung spezifischer hydrologischer Probleme zu analysieren. Zu diesem Zweck wurden in dieser Doktorarbeit fallspezifische Lösungen für verschiedene Untersuchungsgebiete entwickelt, die sowohl in der räumlichen Skale als auch in den zu untersuchenden hydrologischen Fragestellungen eine große Diversität aufweisen.
Im ersten Teil der Arbeit wurde die Massenbilanz von Wasserinhaltsstoffen in einem meromiktischen Tagebaurestsee im Lausitzer Revier durch physikalische und mathematische Modellierungsmethoden untersucht. Dabei konnte gezeigt werden, dass auf Basis einer gewonnenen mehrjährigen Zeitreihe von Messdaten ein einfaches Massenbilanzmodell in der Lage ist, sowohl Seeschichtungs- als auch Grundwasseraustauschdynamiken quantitativ zu beschreiben.
Der zweite Teil der Arbeit umfasst die Entwicklung eines transienten numerischen Grundwassermodells für den quartären Uferaquifer im Bereich eines Flussmäanders der Selke welches anhand von Daten aus mehreren Salztracertests kalibriert wurde. Das Modell wurde dafür verwendet die transienten Verweilzeiten in der gesättigten Zone des Mäanderbogens unter dem Einfluss dynamischer hydraulischer Bedingungen zu untersuchen. Die Ergebnisse wurden im Anschluss mit Verweilzeiten verglichen, die aus der Analyse der zeitlichen Verschiebung von gemessenen elektrischen Leitfähigkeitszeitreihen zwischen Fluss und Grundwassermessstellen gewonnen wurden. Durch dieses kombinierte Verfahren konnten sowohl die Beschränkungen der zeitreihenbasierten Verweilzeitberechnung aufgezeigt als auch ein tieferes Systemverständnis für die Interaktionsdynamiken zwischen Grund- und Flusswasser auf der Mäanderskala gewonnen werden.
Der dritte Teil der Arbeit beschreibt die Vorgehensweise für die Bewertung des Grundwasserkontaminationsrisikos im Einzugsgebiet des Songhua Flusses in China. Eine umfassende Literaturstudie wurde durchgeführt, um einen Überblick über die Verfügbarkeit von Messdaten zur Belastung der Wasserressourcen Chinas mit organischen Schadstoffen zu erhalten. Danach wurde für ein Teileinzugsgebiet ein dreidimensionales numerisches Grundwassermodell auf Basis der vorhandenen hydrogeologischen Daten aufgebaut. Dieses wurde dazu verwendet die Änderungen im Stofftransports und den Schadstoffkonzentrationen innerhalb des Aquifersystems unter steigenden Entnahmeraten zu analysieren. Basierend auf diesen Studien wurden auf der Skale des Gesamteinzugsgebiets, um die beschränkte Verfügbarkeit von Felddaten auszugleichen, die Ergebnisse der numerischen Grundwassermodellierung mit Fernerkundungsdaten und Webdatenbanken in einem Indexsystem kombiniert mit dem für die oberflächennahen Aquifere Vulnerabilität, Gefährdungspotential und Verschmutzungsrisiko in einer räumlichen Auflösung von 1 km² bestimmt wurden.
Zusammenfassend konnten durch die vorliegende Doktorarbeit neue passgenaue Methoden zur effektiven Kombination von in-situ Messungen, der Datenerhebung und Datenintegration aus vielfältigen Datenquellen sowie numerischen Grundwassermodellierungsstrategien entwickelt und zur Lösung der untersuchten hydrologischer Fragestellen auf den verschiedenen Skalen und über die Grenzen der einzelnen hydrologischen Teilsysteme hinaus erfolgreich angewandt werden.
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Combining measurements, remote sensing and numerical modelling to assess multi-scale flow dynamics in groundwater-dependent environmental systemsNixdorf, Erik 02 March 2018 (has links)
Groundwater flow modelling provides an important quantitative instrument for addressing issues related to the quantity and quality of groundwater and the connected water resources. Consequently, groundwater flow models have been developed and used ubiquitously in science to deepen the understanding of subsurface processes and their drivers as well as management and planning tools.
The present work investigates how numerical models can be linked to field investigations and public databases to quantitatively approach questions in the area of groundwater research. The primary goal is to develop new, efficient ways to overcome limitations of the individual hydrological concepts for solving specific hydrological problems and to increase the understanding of practical applicability of different methods. For this purpose, tailor-made approaches were developed for different study areas covering diverse spatial scales: the hydrology of a small mining lake, the riparian aquifer at the scale of a single meander as well as the aquifer systems of a large-scale river basin in China.
The first part of the work deals with the physical and mathematical modelling of water constituents balance in a meromictic mining lake in Lusatia. The capability of using a rather simple mass-balance model based on a sufficient dataset of field data to evaluate lake stratification and lake-groundwater interaction were shown.
In the second part, a transient numerical groundwater flow model was developed for the riparian aquifer of a stream meander and was calibrated by three different salt tracer tests. The model was used to proof the reliability of subsurface travel times derived from time series analysis and to give insights in the riparian zone dynamics during changing hydraulic gradients.
The third part of the work describes the methodology to conduct risk assessment of groundwater contamination on the large catchment scale of the Songhua River in China. A comprehensive literature study was conducted to get an overview about measurement data on water quality data in China. A three-dimensional numerical flow and mass transport model was applied to access the flow and matter transport dynamics in the aquifer system of a sub-basin considering changing groundwater exploitation scenarios. Consequently, numerical groundwater modelling was combined with processed remote sensing and web mapping service data to overcome field data limitations and to derive groundwater vulnerability, groundwater hazard and groundwater risk maps for the entire Songhua River Basin.
Summarizing, this doctoral thesis could develop new methods of combining field measurements, data assimilation and aggregation from various sources and groundwater modelling strategies and successfully apply these methods to find solutions on problems of multiple scales and across water systems. / Die Grundwassermodellierung stellt eine wichtige wissenschaftliche Methode zur quantitativen Analyse von Fragestellungen zum Schutz der Menge und Güte der Grundwasserressourcen sowie der angeschlossenen Wasserkörper dar. Dementsprechend werden Grundwassermodelle sowohl für Planungs- und Bewertungszwecke im Wasserressourcenmanagement als auch zur wissenschaftlichen Erforschung der Prozesse im Untergrund entwickelt und angewendet.
Die vorliegende Arbeit untersucht in diesem Rahmen, wie numerische Modelle, Feldmessungen und Daten generiert aus Fernerkundungsdaten und Webplattformen systematisch verknüpft werden können, um Fragestellungen im Bereich der Grundwasserforschung quantitativ zu beantworten. Das Ziel der Arbeit ist es neue effiziente Abläufe zu entwickeln, die die Limitierung der einzelnen Methoden überwinden und diese auf deren Anwendbarkeit für die Lösung spezifischer hydrologischer Probleme zu analysieren. Zu diesem Zweck wurden in dieser Doktorarbeit fallspezifische Lösungen für verschiedene Untersuchungsgebiete entwickelt, die sowohl in der räumlichen Skale als auch in den zu untersuchenden hydrologischen Fragestellungen eine große Diversität aufweisen.
Im ersten Teil der Arbeit wurde die Massenbilanz von Wasserinhaltsstoffen in einem meromiktischen Tagebaurestsee im Lausitzer Revier durch physikalische und mathematische Modellierungsmethoden untersucht. Dabei konnte gezeigt werden, dass auf Basis einer gewonnenen mehrjährigen Zeitreihe von Messdaten ein einfaches Massenbilanzmodell in der Lage ist, sowohl Seeschichtungs- als auch Grundwasseraustauschdynamiken quantitativ zu beschreiben.
Der zweite Teil der Arbeit umfasst die Entwicklung eines transienten numerischen Grundwassermodells für den quartären Uferaquifer im Bereich eines Flussmäanders der Selke welches anhand von Daten aus mehreren Salztracertests kalibriert wurde. Das Modell wurde dafür verwendet die transienten Verweilzeiten in der gesättigten Zone des Mäanderbogens unter dem Einfluss dynamischer hydraulischer Bedingungen zu untersuchen. Die Ergebnisse wurden im Anschluss mit Verweilzeiten verglichen, die aus der Analyse der zeitlichen Verschiebung von gemessenen elektrischen Leitfähigkeitszeitreihen zwischen Fluss und Grundwassermessstellen gewonnen wurden. Durch dieses kombinierte Verfahren konnten sowohl die Beschränkungen der zeitreihenbasierten Verweilzeitberechnung aufgezeigt als auch ein tieferes Systemverständnis für die Interaktionsdynamiken zwischen Grund- und Flusswasser auf der Mäanderskala gewonnen werden.
Der dritte Teil der Arbeit beschreibt die Vorgehensweise für die Bewertung des Grundwasserkontaminationsrisikos im Einzugsgebiet des Songhua Flusses in China. Eine umfassende Literaturstudie wurde durchgeführt, um einen Überblick über die Verfügbarkeit von Messdaten zur Belastung der Wasserressourcen Chinas mit organischen Schadstoffen zu erhalten. Danach wurde für ein Teileinzugsgebiet ein dreidimensionales numerisches Grundwassermodell auf Basis der vorhandenen hydrogeologischen Daten aufgebaut. Dieses wurde dazu verwendet die Änderungen im Stofftransports und den Schadstoffkonzentrationen innerhalb des Aquifersystems unter steigenden Entnahmeraten zu analysieren. Basierend auf diesen Studien wurden auf der Skale des Gesamteinzugsgebiets, um die beschränkte Verfügbarkeit von Felddaten auszugleichen, die Ergebnisse der numerischen Grundwassermodellierung mit Fernerkundungsdaten und Webdatenbanken in einem Indexsystem kombiniert mit dem für die oberflächennahen Aquifere Vulnerabilität, Gefährdungspotential und Verschmutzungsrisiko in einer räumlichen Auflösung von 1 km² bestimmt wurden.
Zusammenfassend konnten durch die vorliegende Doktorarbeit neue passgenaue Methoden zur effektiven Kombination von in-situ Messungen, der Datenerhebung und Datenintegration aus vielfältigen Datenquellen sowie numerischen Grundwassermodellierungsstrategien entwickelt und zur Lösung der untersuchten hydrologischer Fragestellen auf den verschiedenen Skalen und über die Grenzen der einzelnen hydrologischen Teilsysteme hinaus erfolgreich angewandt werden.
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