Submitted by Aelson Maciera (aelsoncm@terra.com.br) on 2017-09-12T20:08:39Z No. of bitstreams: 1 DissMGL.pdf: 1331735 bytes, checksum: d89c5c7a512c9941230916fb87f00426 (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-09-25T11:53:36Z (GMT) No. of bitstreams: 1 DissMGL.pdf: 1331735 bytes, checksum: d89c5c7a512c9941230916fb87f00426 (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-09-25T11:53:44Z (GMT) No. of bitstreams: 1 DissMGL.pdf: 1331735 bytes, checksum: d89c5c7a512c9941230916fb87f00426 (MD5) / Made available in DSpace on 2017-09-25T11:57:45Z (GMT). No. of bitstreams: 1 DissMGL.pdf: 1331735 bytes, checksum: d89c5c7a512c9941230916fb87f00426 (MD5) Previous issue date: 2017-02-22 / Não recebi financiamento / The present work seeks the understanding of how the culture is constituted in the official and unofficial scope of the state of. It focused on the cultural legislations of the state and its dialogical and ideological relationship with the cultures of resistance. Accordingly, Law No. 12,268 of February 20, 2006 (SÃO PAULO, 2006a) and Law No. 15,565, of September 25, 2014 (SÃO PAULO, 2014) were selected and analyzed from a Bakhtin perspective. In this analysis the statements of the legislations were compared with official texts and texts that defend or belong to the culture of resistance. It can be seen from the analysis that the State has no direct official actions and links with this culture. The proposal is the practice of a more democratic legislation that meets the real needs of the communities. Mainly in the defense and use of popular culture. / O presente trabalho busca a compreensão de como a cultura é constituída nos âmbitos oficial e não oficial do estado de São Paulo. Teve como foco as legislações culturais do estado e a sua relação dialógica e ideológica com as culturas de resistência. Desta maneira, as Lei n°12.268, de 20 de fevereiro de 2006 (SÃO PAULO, 2006a) e a Lei n°15.565, de 25 de setembro de 2014 (SÃO PAULO, 2014) foram selecionadas e analisadas na perspectiva Bakhtiniana. Nesta análise os enunciados das legislações foram cotejados com textos oficiais e com textos que defendem ou pertencem à cultura de resistência. Percebe-se com as análises que o Estado não possui ações e ligações oficiais diretas com esta cultura. A proposta é a prática de um legislar mais democrático e que atenda as reais necessidades das comunidades. Principalmente na defesa e uso da cultura popular.

387f. / Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-04-22T16:15:04Z No. of bitstreams: 3 Tese Ileana Limonta3.pdf: 3908228 bytes, checksum: f95d8bba1c5ec0e4d8ef9961db5dc65d (MD5) Tese Ileana Limonta2.pdf: 5969335 bytes, checksum: 71ada33384664a2717a4be6bfe0c8137 (MD5) Tese Ileana Limonta1.pdf: 8530405 bytes, checksum: f35dc1d3ce53f4921cd5e8bfcb94ed7a (MD5) / Approved for entry into archive by Rodrigo Meirelles(rodrigomei@ufba.br) on 2013-05-24T11:39:35Z (GMT) No. of bitstreams: 3 Tese Ileana Limonta3.pdf: 3908228 bytes, checksum: f95d8bba1c5ec0e4d8ef9961db5dc65d (MD5) Tese Ileana Limonta2.pdf: 5969335 bytes, checksum: 71ada33384664a2717a4be6bfe0c8137 (MD5) Tese Ileana Limonta1.pdf: 8530405 bytes, checksum: f35dc1d3ce53f4921cd5e8bfcb94ed7a (MD5) / Made available in DSpace on 2013-05-24T11:39:35Z (GMT). No. of bitstreams: 3 Tese Ileana Limonta3.pdf: 3908228 bytes, checksum: f95d8bba1c5ec0e4d8ef9961db5dc65d (MD5) Tese Ileana Limonta2.pdf: 5969335 bytes, checksum: 71ada33384664a2717a4be6bfe0c8137 (MD5) Tese Ileana Limonta1.pdf: 8530405 bytes, checksum: f35dc1d3ce53f4921cd5e8bfcb94ed7a (MD5) Previous issue date: 2009 / Esta tese tem como enfoque o estudo comparativo da Santería cubana e dos Candomblés brasileiros de tradição iorubá. O objetivo foi estudar os perfis identitários destas expressões religiosas de matriz africana, como cultura de resistência ante uma cultura imposta durante séculos de colonialismo pelas elites do poder, no período histórico que vai de 1950 a 2000. Teve-se em vista os mecanismos de enfrentamento ou confrontação que estas religiosidades criaram como via de defesa e inserção social. Tentamos desvendar, na medida do possível e de forma comparativa, as relações políticas e a incidência sócio-cultural no entorno social em que atuaram estas expressões em Cuba e no Brasil, conhecendo de antemão a influência que tradicionalmente elas têm exercido na esfera da consciência individual dos iniciados em seus cultos religiosos. Temas como: africanidade, etnicidade, cultura, resistência, poder e repressão foram analisados em função da estrutura religiosa da Santería e do Candomblé, em contrate com a realidade social que enfrentaram nos países para onde foram transplantadas. Esta estrutura teve como núcleo principal a família religiosa em uma intrincada rede de sociabilidades, que encontrou e ainda encontra nos terreiros e nas casas-templo o ambiente propício para recriar os elementos culturais africanos que sustentam, como parte do continuum das tradições culturais herdadas dos africanos, que buscaram e encontraram um lugar na história e identidade nacional cubana e brasileira, como expressões religiosas de resistência em defesa da identidade cultural. / Salvador

Made available in DSpace on 2016-04-27T19:30:27Z (GMT). No. of bitstreams: 1 Horacio dos Reis Marques Ferreira.pdf: 4832695 bytes, checksum: 463b854cb07943c7624c75185cae8ff9 (MD5) Previous issue date: 2011-10-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / In this research I sought to understand the culture of boias-frias workers of Pontalinda town, São Paulo State, as way of life, values, habits, customs and work. It undertakes the period of 1991 to 2010, when Boia-frias population had settled in urban space as a result of rural exodus and the presence of migrants from the Northeast, with a predominance of Bahia s people. The work enabled me to hear and interpret the trajectories and cultures experienced by boia-fria workers, to understand how these social actors give meanings to the present and the past which is redesigned according to their experiences today. I sought to investigate the relationship instituted in urban space, the ways of living, housing, fighting, working and recreation of the workers that with their actions were registering their brands. The narratives record the history of struggle and resistance of these workers in search of space and survival within society, and value the memory of those ones, who are the main labor force for the development of the region, so far. The dialogue with oral sources, the written ones (newspapers, magazines, official documents, labor, criminal) and photographs, enhanced the memory of the Boia-frias people, bringing out other stories and other views on the subject of past and present of these people. The issues raised and the dialogues with evidence, thinking about this story as unique in an enclosed space and built by humans unique, led me to reflect on the full history as lived experience and socially, in a field of contradictions, domination and resistance. As E.P. Thompson, I do not start from hypotheses and look for sources to confirm them. Rather, I start from the sources, the issues raised by evidence, in order to question the Boia-frias people of Pontalinda town of São Paulo / Nesta pesquisa, busquei compreender a cultura dos trabalhadores boias-frias da cidade de Pontalinda, Estado de São Paulo, enquanto modo de vida, valores, hábitos, costumes e trabalho. Ela teve como universo temporal o período de 1991 a 2010, quando já havia se consolidado uma população de boias-frias no espaço urbano, resultante do êxodo rural e da presença de migrantes nordestinos, com predominância de baianos. O trabalho possibilitou-me ouvir e interpretar as trajetórias e culturas vividas pelos trabalhadores boias-frias, compreender como estes sujeitos sociais atribuem significados ao presente e ao passado, que é reelaborado conforme suas vivências atuais. Busquei investigar nas relações instituídas no espaço urbano os modos de viver, morar, lutar, trabalhar e divertir-se dos trabalhadores, que, com suas ações, foram registrando suas marcas. As narrativas, aqui representadas, registram a história de luta e resistência desses trabalhadores na busca de espaço e sobrevivência dentro da sociedade e valorizam a memória desses sujeitos que constituem a principal da força de trabalho para o desenvolvimento da região, até então. O diálogo com fontes orais, escritas (jornais, revistas, documentos oficiais, ações trabalhistas, processos crimes) e fotografias, valorizou a memória dos boias-frias, trazendo à tona outras histórias e outros olhares sobre o passado e presente desses sujeitos. As problemáticas levantadas e os diálogos com as evidências, pensando essa história como única, em um espaço delimitado e construído por seres humanos únicos, levaram-me a refletir sobre a história como experiência vivida socialmente, num campo de contradições, dominação e resistência. Conforme E. P. Thompson, não parto de hipóteses e procuro as fontes para confirmá-las. Parto das fontes, de questões colocadas pelas evidências para problematizar1 os boias-frias da cidade de Pontalinda-SP

Patient safety is of particular importance within intensive care units (ICUs), where critically ill, vulnerable patients receive complex multidisciplinary care. Prior research has indicated that improving patient safety and reducing errors within healthcare requires a focus on systems and organisational culture issues. This thesis was concerned with three studies. One focused on assessing the patient safety culture and two on quality improvement initiatives within an intensive care unit (ICU) of a large teaching hospital. The first study involved a survey of ICU consultant, registrar and nursing staff regarding aspects of safety culture. This was conducted using an existing Hospital Survey on Patient Safety Culture. Of the twelve patient safety culture composites assessed, eight had scores lower than 50%, highlighting these as areas for improvement. Overall, while the survey results revealed that teamwork within the ICU was considered a strength, event reporting and patient care handovers and transitions were both considered areas with potential for improvement. The second study focused on the evaluation of a change initiative designed to improve the handover of patient clinical information in the ICU. This study involved a survey and interviews with consultant, registrar and nursing staff before and after the introduction of a Patient Management, Plan and Progress (PMPP) document. Examination of the survey responses involved both quantitative and qualitative analysis; respondent interview transcripts were analysed using thematic analysis. The results of this study revealed resistance to, and criticisms of, the introduction of the PMPP document; the initiative failed and use of the document was discontinued. The second initiative concerned an evaluation of the impact of a hospital-wide document on improving documentation of withdrawal of patient treatment within the ICU. This involved both quantitative and qualitative analysis, with a patient medical record audit of decisions to withdraw patient treatment within the ICU before and after the introduction of an Advance Care Plan (ACP) document. ICU consultant, registrar and nursing staff were interviewed regarding the process of withdrawal of patient treatment within the ICU. Interview transcripts were analysed using a modified grounded theory approach. Results revealed that the attempt to improve the documentation of withdrawal of treatment within the ICU failed, with the ACP document remaining unused in 89% of cases and incomplete in the remaining 11%. Also, documentation of decision-making and of the process within the medical records did not improve. Before-introduction findings revealed that only 26% of medical records met the pre-existing requirements for treatment withdrawal in the ICU, and after-introduction findings revealed that only 19% of medical records audited met the requirements of the ACP document. After-audit findings also revealed significant and inappropriate increases in the involvement of an ICU registrar both as primary and secondary decision-makers. In spite of an increased awareness of ICU staff concerning the importance of improving documentation, the medical record audit revealed less compliance with the standards required for documentation. Possible reasons for the document remaining essentially unused, as revealed from interviews with staff, included: previous criticisms by the coroner when they failed to complete a similar formalised document properly; perceived logistical issues associated with obtaining required staff signatures; disagreement concerning who should be involved in documenting the withdrawal of treatment process; and the existence of an ICU subculture of practice that, in one particular aspect of documentation, was not consistent with established hospital and ICU protocol and documentation requirements. The final chapter of this thesis considered implications of the results of the studies for the planning, development, implementation and evaluation of improvement programs within the ICU setting. The results were considered within the context of organisational change management theory and research, including factors that have been found to be critical in the success or failure of change programs, such as resistance to change, the involvement of key stakeholders in the change process, leadership, communication and organisational culture. It is suggested that management consultants with organisational change expertise in the planning, development, implementation and evaluation of such programs should be involved in future quality improvement initiatives. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297608 / Thesis (Ph.D.) -- University of Adelaide, School of Psychology, 2007

Patient safety is of particular importance within intensive care units (ICUs), where critically ill, vulnerable patients receive complex multidisciplinary care. Prior research has indicated that improving patient safety and reducing errors within healthcare requires a focus on systems and organisational culture issues. This thesis was concerned with three studies. One focused on assessing the patient safety culture and two on quality improvement initiatives within an intensive care unit (ICU) of a large teaching hospital. The first study involved a survey of ICU consultant, registrar and nursing staff regarding aspects of safety culture. This was conducted using an existing Hospital Survey on Patient Safety Culture. Of the twelve patient safety culture composites assessed, eight had scores lower than 50%, highlighting these as areas for improvement. Overall, while the survey results revealed that teamwork within the ICU was considered a strength, event reporting and patient care handovers and transitions were both considered areas with potential for improvement. The second study focused on the evaluation of a change initiative designed to improve the handover of patient clinical information in the ICU. This study involved a survey and interviews with consultant, registrar and nursing staff before and after the introduction of a Patient Management, Plan and Progress (PMPP) document. Examination of the survey responses involved both quantitative and qualitative analysis; respondent interview transcripts were analysed using thematic analysis. The results of this study revealed resistance to, and criticisms of, the introduction of the PMPP document; the initiative failed and use of the document was discontinued. The second initiative concerned an evaluation of the impact of a hospital-wide document on improving documentation of withdrawal of patient treatment within the ICU. This involved both quantitative and qualitative analysis, with a patient medical record audit of decisions to withdraw patient treatment within the ICU before and after the introduction of an Advance Care Plan (ACP) document. ICU consultant, registrar and nursing staff were interviewed regarding the process of withdrawal of patient treatment within the ICU. Interview transcripts were analysed using a modified grounded theory approach. Results revealed that the attempt to improve the documentation of withdrawal of treatment within the ICU failed, with the ACP document remaining unused in 89% of cases and incomplete in the remaining 11%. Also, documentation of decision-making and of the process within the medical records did not improve. Before-introduction findings revealed that only 26% of medical records met the pre-existing requirements for treatment withdrawal in the ICU, and after-introduction findings revealed that only 19% of medical records audited met the requirements of the ACP document. After-audit findings also revealed significant and inappropriate increases in the involvement of an ICU registrar both as primary and secondary decision-makers. In spite of an increased awareness of ICU staff concerning the importance of improving documentation, the medical record audit revealed less compliance with the standards required for documentation. Possible reasons for the document remaining essentially unused, as revealed from interviews with staff, included: previous criticisms by the coroner when they failed to complete a similar formalised document properly; perceived logistical issues associated with obtaining required staff signatures; disagreement concerning who should be involved in documenting the withdrawal of treatment process; and the existence of an ICU subculture of practice that, in one particular aspect of documentation, was not consistent with established hospital and ICU protocol and documentation requirements. The final chapter of this thesis considered implications of the results of the studies for the planning, development, implementation and evaluation of improvement programs within the ICU setting. The results were considered within the context of organisational change management theory and research, including factors that have been found to be critical in the success or failure of change programs, such as resistance to change, the involvement of key stakeholders in the change process, leadership, communication and organisational culture. It is suggested that management consultants with organisational change expertise in the planning, development, implementation and evaluation of such programs should be involved in future quality improvement initiatives. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297608 / Thesis (Ph.D.) -- University of Adelaide, School of Psychology, 2007

abstract: The utopian impulse represents hope for another world; a reflection of the injustices inherent to the hegemonic order that are understood as natural, necessary, desirable, and unchangeable. Those who challenge this orthodoxy are heretical utopians; pioneers of the counterintuitive who explore the types of relations that rather than reproduce the dominant order, shatter it, and manifest new ones based upon principles of justice. This project explores how ideological mechanisms of control embedded within the hegemonic fascist imaginary landscape of the United States render the visions of emancipatory social movements, that challenge dominant ways of knowing and being, as the "merely utopian" so as to instrumentalize the behavior of civil-society towards the maintenance of the established social order and the suppression of alternatives (Gordon 2004). In a rapidly changing world reeling under the pressures of late-stage capitalism, it is essential for those who value social and political justice to incessantly cultivate the cultural imaginary so as to shift the boundaries of what types of social relations are possible, feasible, and desirable through the process of struggle in heretical spaces. / Dissertation/Thesis / Masters Thesis Justice Studies 2015

Cancer cells have been known for some time to have very different metabolismas compared to that of normal non proliferating cells. As metabolism is involvedin almost every aspect of cell function, there has been a recent resurgence ofinterest in inhibiting cancer metabolism as a therapeutic strategy. Inhibitors thatspecifically target altered metabolic components in cancer cells are being developedas antiproliferative agents. However, many such inhibitors have not progressedinto the clinic due to limited efficacy either in vitro or in vivo. In this study weexplore the hypothesis that this is often due to the robustness of the metabolicnetwork and the differences between individual cancer cell lines in their metaboliccharacteristics. We take a systems biology approach. We investigate the cellular bioenergetic profiles of a panel of five non-small celllung cancer cell lines before and after treatment with a novel inhibitor of theglutaminase-1 (GLS1) enzyme. Additionally, we explore the effects of this inhibitoron intracellular metabolism of these cell lines as well as on the uptake and secretionof glucose, lactate and amino acids. To be able to do the latter robustly, wehad to modify the experimental assay considerably from procedures that seemto be standard in the literature; using these earlier procedures the metabolicenvironment of the cells was highly variable, leading to misleading results onthe metabolic effects of the inhibitor. We reduced cell density, altered mediumvolume and changed the time-window of the assay. This led to the cells growingexponentially, appearing indifferent to the few remaining changes. In this newassay, the metabolic effects of the glutaminase inhibitor became robust. One of the most significant results of this study is the metabolic heterogeneitydisplayed across the cell line panel under basal conditions. Differences in themetabolic functioning of the cell lines were observed in terms of both theirbioenergetic and metabolic profile. The amount of respiration attributed tooxidative phosphorylation differed between cell lines and respiratory capacity wasattenuated in most cells. However, the rate of glycolysis was similar betweencell lines in this assay. These results suggest that the Warburg effect arisesthrough a greater diversity of mechanisms than traditionally assumed, involvingvarious combinations of changes in the expression of glycolytic and mitochondrialmetabolic enzymes. The effects of GLS1 inhibition on cellular bioenergetics and metabolism alsodiffered between cell lines, even between resistant cell lines, indicating that theremay also be a diversity of resistance mechanisms. The metabolomic response ofcell lines to treatment suggests potential resistance mechanisms through metabolicadaptation or through the prior differences in the metabolic function of resistantcell lines. Part of the metabolome response to GLS1 inhibition was quite specificfor sensitive cells, with high concentrations of IMP as the strongest marker. Our results suggest that the metabolome is a significant player in what determinesthe response of cells to metabolic inhibitors, that its responses differ between cancercells, that responses are not beyond systems understanding, and that thereforethe metabolome should be taken into account in the design of and therapy withanti-cancer drugs.

Microbes typically form highly complex and diverse communities that account for a significant portion of life's genetic diversity. Analysis of living systems, e.g. bacterial or cell population, plays a significant role in detecting and identifying pathogens, testing antibiotic susceptibility, and the fundamental research of population diversity and evolution. This work focuses on the analysis of bacterial communities using droplets based millifluidics. To monitor the bacteria growth, we designed an optofluidic system, combining the encapsulation of bacteria in numerous emulsion droplets to monitor their long-term behavior and relationship in a co-culture environment using fluorescent signals. In the first part of this work, we co-encapsulated and cultured two isogenic strains of Escherichia coli (E. coli) in numerous emulsion droplets to reveal their competition and cooperation relationship. Since two strains of E. coli express blue and yellow fluorescent proteins (BFP and YFP, respectively), we quantified their growth by integrating a fluorescence detection system. We analyzed the following parameters: doubling time, population yield, final biomass ratio, correlation map of doubling time and competition coefficient to characterize and compare the bacterial growth kinetics and behavior in mono and co-cultures. In addition, the experimental observations were compared with the predictions from a single growth model. Finally, we employed the millifluidic device to verify the appearance of cross-protection between antibiotic-sensitive bacteria and antibiotic-resistant bacteria. It is one of the mechanisms by which different bacteria, sharing the same environment, protect each other to survive in the presence of antibiotics. For this purpose, the E.coli YFP strain was chosen as an antibiotic-sensitive group. Simultaneously, the E.coli BFP strain with β-lactam and its mutations were selected as resistant strains. Combining the millifluidic droplet reactor method with other detection strategies, e.g. fluorescence microscopy, fluorescence flow cytometry, and plate reader, we proved the appearance of cross-protection by detecting the filamentary cells, the fluorescence of cell-free media, viable cell rates, cell shape and size, as well as β-lactamase activity. All these results obtained by millifluidic devices proved that this strategy could be used in a high-throughput bacterial coexistence study. In addition, the research of these general fields, such as bacterial community and antibiotic impact, can help us to reveal the interaction between microbial species and determine the right dose of antibiotics to inhibit bacterial growth in a co-existent environment efficiently.