Spelling suggestions: "subject:"cytoskeleton."" "subject:"zytoskeleton.""
261 |
Inibição da migração mediada pelo gene RECK em modelo de glioma humano através de alterações no citoesqueleto e adesão focal / RECK-mediated inhibition of glioma migration with changes in cytoskeleton and focal adhesionRaquel Brandão Haga 18 May 2012 (has links)
Gliomas são tumores altamente invasivos, resistentes aos tratamentos disponíveis atualmente e com alta taxa de mortalidade. A superexpressão de RECK na linhagem de glioma humano T98G comprometeu a capacidade das células de migrar e invadir in vitro, com rearranjo do citoesqueleto e alteração na distribuição espacial de FAK fosforilado. Entretanto, o possível mecanismo envolvido na inibição da migração mediada por RECK não foi desvendado. Para estudarmos os mecanismos envolvidos nesta alteração da capacidade migratória, as células T98G foram transfectadas com o vetor plasmidial pCXN2-hRECK (RECK+). A via das integrinas, a atividade de alguns membros da família das RhoGTPases e elementos do citoesqueleto foram avaliados através de imunoblotting, imunomarcação e ensaios de pull-down para as células RECK+ em comparação com células T98G não-transfectadas (WT), células T98G transfectadas com vetor pCXN2 na ausência do gene RECK (vetor) e fibroblastos primários humanos (FF287). Nossos resultados mostram um aumento na expressão de integrina β1 e uma diminuição da fosforilação de FAK no sítio de auto-fosforilação Tyr397 que, juntamente com o aumento das fibras de estresse e a diminuição dos lamelipódios, sugerem um fenótipo menos migratório da célula. Porém, quando avaliada a atividade de Rac1, esta se mostrou aumentada, embora uma das vias de ativação de Rac1 seja através da fosforilação de FAK levando à formação dos lamelipódios. A hipótese é que RECK inibe a quebra das adesões focais que participam do processo de migração, dificultando a mobilidade celular. Como as células continuam recebendo o estímulo para migrar, estas ativam Rac1 através de uma via independente de FAK. Além disso, a imunomarcação de paxilina mostrou um aumento no tamanho das adesões focais nas células RECK+, indicando que RECK pode influenciar nas estruturas responsáveis pelo contato célula-matriz. / Gliomas are highly invasive, treatment-resistant and lethal tumors. Overexpression of RECK in human glioma cell line T98G decreased cell migration and invasion in vitro, lead to cytoskeleton rearrangement and caused changes in phospho-FAK distribution. However, the pathway involved in RECK-mediated inhibition of cell migration has not been elucidated yet. To study the mechanisms by which RECK affects cell motility, T98G cells were transfected with pCXN2-hRECK vector (RECK+). Some proteins involved in the integrin pathway, activity of some proteins of RhoGTPase family and cytoskeleton proteins were analyzed through immunoblotting, immunostaining and pull-down assay in RECK+ cells and compared with non-transfected T98G cells, T98G transfected with pCXN2 without RECK gene and human primary fibroblasts (FF287). Our results showed an increase in integrin β1 expression and a decrease in FAK phosphorylation in the Tyr397 site, which together with the increase of stress fibers and decrease of lamellipodia, suggest a less migratory phenotype. Despite this, Rac1 activity was increased even though one of Rac activation pathways is through phospho-FAK, leading to lamellipodium formation. Our hypotheses is that RECK affects focal adhesion turnover, diminishing cell motility. As cells are still receiving a positive signal to migrate, they activate Rac1 through a FAK-independent pathway. Besides that, paxillin immunostaining showed that focal adhesions are larger in RECK+ cells, indicating that RECK can influence structures related with cell-matrix contact.
|
262 |
Intercellular calcium-mediated cell signaling in keratinocytes cultured from patients with NF1 or psoriasisKorkiamäki, T. (Timo) 27 September 2002 (has links)
Abstract
Neurofibromatosis type 1 syndrome (NF1) is caused by mutations of
the NF1 gene.
The NF1 protein (neurofibromin) contains a domain which is related to the GTPase-activating protein
(GAP) and accelerates the switch of active Ras-GTP to inactive Ras-GDP. The NF1 protein has been referred
to as a tumor suppressor, since the cells of malignant schwannomas of NF1 patients may display a loss
of heterozygosity of the NF1 gene. Psoriasis is characterized by hyperproliferation of the epidermis and by
down-regulated levels of NF1 mRNA and protein. Ca2+ is an universal signal
transduction element modulating cell growth and differentiation. Many cell types coordinate their activities by
transmitting waves of elevated intracellular calcium levels from cell to cell. The propagation of calcium
waves had not been studied previously in human keratinocytes. Thus, the aim of the present study was to
find out which pathways may play a role in
Ca2+ signaling at different extracellular
calcium concentrations in NF1 and and psoriatic keratinocytes versus
normal control keratinocytes.
The results demonstrated that NF1 and psoriatic keratinocytes have a tendency to form cultures characterized by altered
Ca2+-mediated cell signaling compared to normal keratinocytes. Specifically, the main route of
calcium-mediated signaling was gap-junctional in normal keratinocytes. In contrast, ATP-mediated calcium signaling predominated
and capacitative calcium influx was defective in NF1 and psoriatic keratinocytes.
The results of the present study suggest that mutations of the NF1 tumor
suppressor gene or lowered levels of NF1 protein/mRNA may eventually lead to altered intercellular communication.
|
263 |
Molecular and cell phenotype changes in mitochondrial diseasesAnnunen-Rasila, J. (Johanna) 05 June 2007 (has links)
Abstract
The mitochondrial oxidative phosphorylation system (OXPHOS) generates energy but also deleterious reactive oxygen species (ROS). Changes in the cytoskeleton, composed mainly of microfilaments, microtubules and intermediate filaments, have been observed in OXPHOS deficiency. The 3243A>G point mutation in mitochondrial DNA (mtDNA) leads to mitochondrial encephalomyopathy, lactic acidosis and stroke-like episodes (MELAS), which is the most common mitochondrial disease. Interestingly, mitochondrial aberrations have been demonstrated in patients with a mutation in NOTCH3, the genetic cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Randomization of vimentin intermediate filament direction and length together with slower population growth was observed in myoblasts with 3243A>G, with no difference in the amount of apoptotic cell death. Upon complex IV inhibition (with or without the microtubule-depolymerizing compound nocodazole) or a lack of mtDNA (ρ0) in osteosarcoma cells the vimentin network collapsed perinuclearly, forming thick bundles, whereas complex I inhibition led to thinner vimentin network bundles. Furthermore, the amount of vimentin was increased in ρ0 cells. Mitochondria accumulated around the nucleus upon complex IV inhibition and in ρ0 cells. Analysis of the total proteome revealed that specific OXPHOS deficiencies led to changes in the expression of cytoskeletal proteins and proteins involved in apoptosis, OXPHOS, glycolysis and oxidative stress response. Muscle histochemical and genetic analysis showed ragged red fibres and cytochrome c oxidase-negative fibres to be associated with 5650G>A in a patient with R133C in NOTCH3 and 5650G>A in MTTA. Immunolabelling of cells with R133C and 5650G>A revealed a sparse tubulin network with asters and less abundant mitochondria by comparison with control cell lines. Comparison of nucleotide diversity between CADASIL pedigrees and controls showed increased mtDNA sequence variation in the CADASIL patients. Also maternal relatives in two CADASIL pedigrees differed from each other in their mtDNA.
These findings suggest that defects in OXPHOS lead to selective changes in the vimentin network, which may have a role in the pathophysiology of mitochondrial diseases. They also suggest a relationship between NOTCH3 and mtDNA, and establish the pathogenicity of 5650G>A. The overall results emphasize that a deficiency in the energy converting system together with oxidative stress can lead to cytoskeletal changes.
|
264 |
Effet moléculaire du peptide vecteur (R/W)9 sur le phénotype de cellules modèles du sarcome d'Ewing : étude protéomique / Molecular effect of (R/W)9 cell penetrating peptide on an Ewing sarcoma's model cell line phenotype : a proteomic studyClavier, Severine 16 October 2014 (has links)
L’objectif du projet est de comprendre l’effet du peptide vecteur (R/W)9 sur les cellules tumorales EF, modèles du sarcome d’Ewing. En effet, ce peptide a la capacité de remodeler le cytosquelette d’actine dans ces cellules, ainsi que de réduire leur motilité et leur aptitude à croître en indépendance d’ancrage (Delaroche D. JBC 2010).La première étape de ce travail a été la caractérisation in vitro de l’interaction directe avec l’actine par cross-linking chimique et spectrométrie de masse (Clavier S. EuProt 2014). Ensuite, pour avancer dans la compréhension de l’effet du peptide (R/W)9, deux approches ont été développées.La première approche, basée sur du photocross-linking in cellulo ou in lysat, vise à identifier des partenaires intracellulaires du peptide vecteur. Pour cela, nous avons mis au point et validé biologiquement une version photoactivable du peptide (R/W)9. Puis, avant de passer sur cellules entières, nous nous sommes assurés de la faisabilité de la réaction de photocross-linking in vitro sur un système d’interaction modèle que nous avons également utilisé pour développer un logiciel capable d’interpréter les spectres MS/MS d’espèces photocross-linkées (Xlink-Identifier, Collaboration Dr Du X). Des expériences de purification d’affinité ont également été menées en immobilisant le peptide (R/W)9 sur des billes de streptavidine ensuite mises en présence de lysat cellulaire. Les protéines capturées ont été identifiées par spectrométrie de masse haut-débit. La seconde approche est de la protéomique différentielle avec un marquage SILAC et a pour objectif de mettre en évidence l’influence du peptide vecteur sur l’expression des protéines. / The aim of the project is to understand the effect of (R/W)9 cell penetrating peptide (CPP) on EF tumoral cells, an Ewing sarcoma model cell line. Actually, this peptide is able to remodel the actin cytoskeleton of these cells, to decrease their motility as well as their ability to grow without anchorage (Delaroche D. JBC 2010).The first step of this work was to characterize the in vitro interaction with actin using chemical cross-linking and mass spectrometry (Clavier S. Euprot 2014). Then, in order to get a deeper understanding of (R/W)9 peptide effect, two approaches were developed. The goal of the first approach based on in cellulo or in lysate photocross-linking is to identify (R/W)9 CPP’s partners. To do this, we designed and biologically validated a photoactivable version of (R/W)9 peptide. Then, before starting to work with living cells, we checked the feasibility of in vitro photocross-linking on a model interacting system that we also used to develop a software able to interpret MS/MS spectra of photocross-linked species. (Xlink-Identifier, Collaboration with Dr. X. Du.). Affinity purification experiments were also performed by incubating streptavidin magnetic beads bearing (R/W)9 peptide with cell lysates. Captured proteins were identified using high-throughput mass spectrometry. The second approach is differential proteomic with SILAC labelling and aims at assessing the influence of (R/W)9 CPP on proteins expression.
|
265 |
Rôle de la réorganisation du cytosquelette des cellules T CD4+ à la synapse immunologique dans les fonctions T / Role of cytoskeleton remodeling in CD4+ T cells at the immunological synapse in T cell functionsBohineust, Armelle 26 June 2013 (has links)
L’activation d’un lymphocyte T (LT) CD4+ par une cellule présentatrice d’antigène (CPA) estune étape cruciale pour la mise en place d’une réponse immune adaptatrice efficace contre unpathogène ou une cellule tumorale. Elle nécessite un contact prolongé entre les deux types cellulaires,initié par la reconnaissance, par le récepteur des LT (TCR), d’un complexe CMH-peptide spécifiqueprésenté à la surface de la CPA. Cette interaction LT-CPA induit la formation d’une zone de contactorganisée dans le temps et l’espace, appelée la synapse immunologique. La mise en place de cettestructure entraîne le remodelage des cytosquelettes d’actine et de microtubules dans les LT. Quelquesminutes après la reconnaissance de l’antigène par le TCR, l’actine se polymérise à la zone synaptiqueet le centre organisateur des microtubules (MTOC) se polarise en face de la CPA.Le but du travail de thèse présenté ici a été d’étudier le rôle de la réorganisation ducytosquelette des LT lors de la mise en place de la synapse, dans la sécrétion des cytokines et lemaintien de l’interaction permettant l’activation des LT. Nous nous sommes particulièrementintéressés au rôle de deux protéines qui régulent le remodelage du cytosquelette : la petite RhoGTPaseCdc42 et un de ses partenaires IQGAP1. Cette étude a été réalisée essentiellement dans des LT CD4+primaires humains, grâce au développement d’une approche permettant d’inhiber l’expression de cesdeux protéines par introduction de shRNAs à l’aide de vecteurs lentiviraux.Nous avons ainsi mis en évidence que le remaniement du cytosquelette d’actine à la synapseétait dépendant de Cdc42, et contrôlait : 1/ la formation d’un anneau d’actine polymérisée enpériphérie de la synapse, 2/ le recrutement et la concentration des vésicules contenant l’IFN-g aucentre de la synapse, 3/ la sécrétion de l’IFN-g dans la zone synaptique. Nous avons également montréque la protéine IQGAP1 contrôlait le remaniement de l’actine des LT à la synapse, la signalisation enaval du TCR, et la dynamique de contact entre LT et CPA.Cette étude participe à une meilleure compréhension du rôle du remodelage du cytosqueletted’actine et de sa régulation dans la mise en place de l’interaction entre LT et CPA, l’activation des LTet une de leur fonction clé : la sécrétion de lymphokines. / CD4+ T lymphocyte activation by an antigen presenting cell (APC) is a crucial step in theestablishment of an adaptive immune response against pathogens or tumor cells. It requires contactbetween the two cell types, initiated by the recognition, by the T cell receptor (TCR), of a specificpeptide-MHC complex presented by the APC. This T cell-APC interaction induces the formation of aparticular zone organized in time and space, called the immunological synapse. The establishment ofthis structure induces the remodeling of the actin and microtubule cytoskeleton in T cells. Few minutesafter antigen recognition by the TCR, actin polymerizes at the synaptic zone, and the microtubuleorganizing center (MTOC) polarizes toward the APC.The goal of the work presented here was to study the role of T cell cytoskeleton remodelingduring the establishment of the synapse, in cytokine secretion and in the maintenance of the interactionallowing T cell activation. We mainly studied the role of two proteins regulating the cytoskeleton: thesmall RhoGTPase Cdc42 and one of its partners IQGAP1. This study was performed mostly in humanprimary CD4+ T cells, thanks to the development of an approach allowing the inhibition of theexpression of these two proteins by introducing shRNAs through lentiviral vectors.Altogether, our results show that remodeling of the actin cytoskeleton at the synapse isdependent on Cdc42 and controls : 1/ the formation of a polymerized actin ring at the periphery of thesynapse, 2/ the recruitment and concentration of vesicles containing IFN-g at the center of the synapse,3/ the secretion of IFN-g in the synaptic cleft. They also show that IQGAP1 controls T cell actinremodeling at the synapse, signaling downstream the TCR, and the dynamic of interactions between Tcells and APC.This study allows a better understanding of the role of cytoskeleton remodeling and of itsregulation in the establishment of Tcell-APC interaction, the activation of T cells and one of their keyfunctions: lymphokine secretion.
|
266 |
Substrate specificity of lysyl hydroxylase isoforms and multifunctionality of lysyl hydroxylase 3Risteli, M. (Maija) 19 July 2008 (has links)
Abstract
Lysyl hydroxylase (LH) catalyzes the post-translational formation of hydroxylysines in collagens and collagenous proteins. Three lysyl hydroxylase isoforms, LH1, LH2 and LH3, have been identified from different species. In addition, LH2 has two alternatively spliced forms, LH2a and LH2b. The hydroxylysines have an important role in the formation of the intermolecular collagen crosslinks that stabilize the collagen fibrils. Some of the hydroxylysine residues are further glycosylated.
In this thesis the substrate amino acid sequence specificities of the LH isoforms were analyzed using synthetic peptide substrates. The data did not indicate strict amino acid sequence specificity for the LH isoforms. However, there seemed to be a preference for some sequences to be bound and hydroxylated by a certain isoform.
Galactosylhydroxylysyl glucosyltransferase (GGT) catalyzes the formation of glucosylgalactosylhydroxylysine. In this study, LH3 was shown to be a multifunctional enzyme, possessing LH and GGT activities. The DXD-like motif, characteristic of many glycosyltransferase families, and the conserved cysteine and leucine residues in the N-terminal part of the LH3 molecule were critical for the GGT activity, but not for the LH activity of the molecule.
The GGT/LH3 protein level was found to be decreased in skin fibroblasts and in the culture media of cells collected from members of a Finnish epidermolysis bullosa simplex (EBS) family, which was earlier reported to have a deficiency of GGT activity. In this study, we showed that the reduction of enzyme activity is not due to a mutation or lower expression of the LH3 gene. Our data indicate that the decreased GGT/LH3 activity in cells has an effect on the deposition and organization of the key extracellular matrix components, collagen types VI and I and fibronectin, and these changes are transmitted to the cytoskeletal network. These findings underline LH3 as an important extracellular regulator.
|
267 |
Buněčná lokalizace a charakteristika funkce TTL proteinů u rostlin huseníčku (Arabidopsis thaliana) / Cellular localization and functional characterization of TTL proteins of ArabidopsisSchier, Jakub January 2016 (has links)
This thesis focuses on the study of TTL protein family in the model species Arabidopsis thaliana. It sums up available published data on this so far poorly studied genes and presents newly gained experimental data. Main emphasis is given to TTLs cellular localization in possible relationship to their physiological function. Presented thesis comprises of in silico analysis of TTL proteins, but also the creation and microscopy analysis of TTL-mRuby2 and TTL-GFP fusion proteins in transient and permanent transformants. Powered by TCPDF (www.tcpdf.org)
|
268 |
NF1 tumor suppressor in epidermal differentiation and growth - implications for wound epithelialization and psoriasisKoivunen, J. (Jussi) 03 August 2003 (has links)
Abstract
Neurofibromatosis type 1 (NF1) is a dominantly inherited neurocutaneous disorder caused by mutations in the NF1 gene. Common clinical manifestations associated with NF1 are neurofibromas, café-au-lait macules (CALM), axillary freckling and Lisch nodules of the iris. Other important manifestations are vasculopathy, a variety of osseous lesions, including short stature, scoliosis and pseudoarthrosis, optic gliomas and an increased risk for certain malignancies. The best characterized function of the NF1 gene is to act as a downregulator of Ras proto-oncogene signalling by accelerating the switch of active Ras-GTP into inactive Ras-GDP. The NF1 gene is considered a tumor suppressor since some malignancies may display a loss of heterozygosity or homozygotic inactivation of the gene.
The present study investigated the behaviour and function of the NF1 gene during keratinocyte differentiation, wound healing and psoriasis using human epidermis and epidermal keratinocytes as a model. The NF1 protein was shown to associate with the intermediate filament network during keratinocyte differentiation both in vitro and in vivo, and it is thus suggested to play a role in the cytoskeletal re-organization or in the formation of cell adhesions. NF1 gene expression was also studied in psoriasis, in which keratinocytes are hyperproliferative and cell differentiation is altered. NF1 gene expression was downregulated in psoriatic keratinocytes both in vivo and in vitro, suggesting that the NF1 gene might have role in downregulating keratinocyte proliferation. During epidermal wound healing, NF1 gene expression was increased. However, the process of wound healing showed no apparent differences between NF1 patients and controls. Furthermore, an increased number of cells immunoreactive for active Ras-MAPK was demonstrated in vascular tissues of NF1 patients, but not in epidermal keratinocytes or dermal fibroblasts. The finding suggests that the NF1 protein functions as a Ras-GAP in some, but not all tissues.
|
269 |
Regulation of cell-cell adhesion and actin cytoskeleton in non-transformed and transformed epithelial cellsPalovuori, R. (Riitta) 21 February 2003 (has links)
Abstract
Epithelial cell-cell adhesions have a critical role in morphogenesis, establishment and maintenance of tissue architecture, cell-cell communication, normal cell growth and differentiation. These adhesions are disrupted during malignant transformation and tumour cell invasion. Several kinases, phosphatases and small GTPases regulate cell-cell contacts. In the present work we investigated the dynamics of cell-cell adhesion structures after microinjection of fluorophore tagged vinculin, during transformation caused by an active Src tyrosine kinase and during Helicobacter pylori infection. The regulatory role of Rac GTPase as well as the behaviour of actin and cadherin were analysed in all these conditions.
Microinjection of vinculin into bovine kidney epithelial MDBK cells induced release of actin, cadherin and plakoglobin to cytoplasm of the cells, caused disruption of protein complexes at adherens and tight junctions that finally led to formation of polykaryons. Activated Rac GTPase, in turn, enhanced accumulation of cadherin to membranes and thereby diminished the formation of polykaryons, whereas inactive Rac removed cadherin from membranes. Incorporation of vinculin to lateral membranes took place also in acidifying and depolarising conditions where cell fusions were prevented. Thus, the membrane potential seemed to control fusion ability. In src-MDCK cells, activation of Src kinase led to disintegration of adherens junctions. Clusters of junctional components and bundles of actin were seen at the basal surface already within 30 min after Src activation. p120ctn was the only component of adherens junction whose relocation correlated to its phosphorylation. Inhibition of Src by a specific inhibitor PP2 restored the cubic morphology of the cells and accumulated cadherin back to lateral walls. Still p120ctn remained in cytoplasm and thereby was not responsible for the epithelial phenotype. Activation of Rac GTPase by Tiam1 also increased the amount of cadherin at lateral membranes and maintained the morphology of src-MDCK cells practically normal after activation of Src kinase. In the same way, actin cytoskeleton was reorganised in gastric carcinoma cells in response to infection with H. pylori via activation of Rac signalling pathway. Hence, Rac and cadherin seem to be the major players in the maintenance of epithelial cell morphology.
|
270 |
Vliv hliníkové toxicity na dynamiku rostlinných kortikálních mikrotubulů / The influence of aluminum toxicity on the dynamics of plant cortical microtubulesPohl, Jana January 2017 (has links)
Aluminium toxicity is the main factor limiting plant growth on acid soils. Aluminium inhibits root growth within few minutes after aluminium treatment. The mechanism and primary target of his action is still unknown. In this diploma thesis the effect of aluminium toxicity on dynamics of cortical microtubules WT and pldα1 plants was studied using the EB1a-GFP marker. Polymerization rate in both the transition and the elongation zone increased immediately after the aplication of aluminium. Nevertheless, microtubules in the transition zone are much more sensitive to aluminium, because the aluminium-induced increase in the polymerization rate was higher than in the elongation zone. Plants lacking PLDα1 showed higher dynamics on plus ends of cortical microtubules compared to WT during aluminium stress, which enabled them to react faster to stress stimuli. Mutants showed lower sensitivity to aluminium and 100 μM concentration of aluminium ions has beneficial effect on root growth in pldα1. These results suggest that PLDα1 influences microtubule dynamics. Microtubules in pldα1 plants were more dynamic and they polymerized faster in the response to aluminium, which was accompanied by decreased sensitivity to aluminium stress compared to WT. Changes in microtubule dynamics may play a role in aluminium...
|
Page generated in 0.0518 seconds