In this study, a two-bed electrically-heated regenerative thermal oxidizer (RTO) was used to test NOx formation characteristics from burning air-laden N, N-dimethyl formamide (DMF) and air-laden DMF mixed with methyl ethyl ketone (MEK). The RTO contained two 0.152 m ¡Ñ 0.14 m ¡Ñ 1.0 m (L ¡Ñ W ¡Ñ H) beds both packed with gravel particles of around 1.11 cm in average diameter to a height of 1.0 m, and the packed section had a void fraction of 0.416. Performances on the thermal destructions of DMF and MEK, the thermal recovery efficiency, as well as the gas pressure drop over the regenerative beds were investigated. Experimental results indicate that, with a valve shifting time (ts) of 1.5 min, gas superficial velocities (Ug) of 0.39-0.78 m/s (evaluated at an influent air temperature of around 30oC), and set maximum destruction temperatures (Tset) of 750-950 oC, there was no NOx in the effluent gas from the RTO with no DMF in the influent air. With only DMF in the influent gas, its destruction efficiencies were 96.3 (750oC), 97.4 (850oC) and 97.9 % (950oC), and increased with increasing influent DMF concentration from 100-250 ppm. Mole ratios of ¡§NOx-N formation/DMF destruction¡¨ were found to be in the range of 0.84-1.20, and the ratio decreased with increasing influent DMF concentration within the experimental range. With both DMF and MEK in the influent gas, no significant influence was found in the NOx formation ratio and the DMF destruction efficiency with influent MEK/DMF ratios of 50/100 - 1500/100 (ppm/ppm) and the set temperatures. The NOx formation ratios were in the range of 0.85-1.07. The Ergun equation was adequate for the estimation of the pressure drop for the gas flowing over the packed regenerative beds in the Ug range of 37-0.74 m/s. It was also found that the thermal recovery efficiency was decreasing with the increasing Ug and invariant with Tset.

In MCF7 and T47D cells cotreated with 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) plus 0.1-10 μM 3’,4’-dimethoxy flavone (DMF), there was a concentration-dependent decrease in the TCDD-induced ethoxyresorufin O-deethylase (EROD) activity. Gel mobility shift assays showed that 3’,4’-DMF inhibited TCDD-induced aryl hydrocarbon receptor (AhR) transformation in rat liver cytosol and blocked TCDD-induced formation of the nuclear AhR complex in MCF7 and T47D cells. The antiestrogenic activity of TCDD in estrogen-induced transactivation assays in MCF7 cells was reversed by 3’,4’-DMF, confirming the AhR antagonist activity of this compound in breast cancer cells. Cotreatment of T47D and MCF7 cells with TCDD and 10 μM resveratrol inhibited induction of CYP1A1 mRNA and EROD activity. Resveratrol did not inhibit TCDD-induced AhR transformation and reporter gene activity. Actinomycin D chase experiments in T47D cells showed that the mechanism of inhibition of CYP1A1 mRNA and EROD activity is due to an increased rate of CYP1A1 mRNA degradation, suggesting that resveratrol inhibits CYP1A1 via an AhR-independent post-transcriptional pathway. Vitamin D receptor-interacting protein 150 (DRIP150) coactivated estrogen receptor α (ER α)-mediated transactivation and the response was AF2-dependent in ZR75 breast cancer cells. C-and N-terminal NR-boxes (amino acids 1186-1182 and 73-69, respectively) were not necessary for coactivation of ERα. Analysis of DRIP150 deletion mutants identified a 23 amino acid sequence (811-789) required for coactivation. The 23 amino acid contained two regions at amino acids 789-794 and 795-804 which resembled α-helical motifs identified in Lanuguinosa lipase/histamine N-methyl transferase and hepatocyte nuclear factor 1, respectively. A squelching assay using specific point mutations within each α-helix showed that the NIFSEVRVYN (795-804) region was the critical sequence required for the coactivator activity of DRIP150.

This study investigates the operation of dual media filter DMF during ambient and simulated algal bloom conditions, and the role of coagulation and dissolved air flotation (DAF) in mitigating the adverse effects of algal blooms on DMF performance. The study also highlights which AOM concentration as a function of biopolymer is critical to organic fouling in DMF pretreatment for Red Sea water desalination with RO. On the other hand, the present study has carried out another experiment on AOM fouling in comparison with bacterial organic matter (BOM) and humic organic matter (HOM) using two different pore sizes of UF ceramic membranes, 5 and 50 kDa. The main aim of this comparison is to examine fouling behavior and mechanism and removal efficiency. The study revealed that AOM can induce organic fouling in DMF during simulated algal bloom conditions at biopolymer concentrations as low as 0.2 mg C/L. DMF performance was strongly affected by AOM concentration as observed by flow rate decline through time. Liquid chromatography – organic carbon detection (LC-OCD) analysis showed higher removal rates of biopolymers than lower molecular weight fractions (i.e., humic substances, building blocks and low molecular weight neutrals) for all pretreatment scenarios. The study also indicated that while DMF performance was enhanced with coagulation and sedimentation, the most significant improvement in performance was observed for DMF operation preceded by coagulation and DAF. Hydraulic performance of DMF correlated well with biopolymers removal, with removal rates of 72%, 53% and 39%, for coagulation/DAF, coagulation/sedimentation, and no coagulation, respectively. For UF ceramic membranes, results showed that more TEP/organics were removed by the 5 kDa membranes compared to the 50 kDa membrane, which is accounted for lower MWCO. The UF 5 kDa membrane also showed low fouling formation than 50 kDa membrane for all of three types of organic matter tested. Analysis of the fouled membranes by SEM images showed that fouling was dominated by cake layer formation for the 5 kDa membrane while pore blockage followed by cake layer formation is apparent for the 50 kDa membrane.

AIM. The aim of the present study was to determine the relationship between diet and dental caries among adolescent school children, between the age group of 13 to 14 years in the city of Chennai, India OBJECTIVES. To find out the characteristics of diet pattern among school children; to analyze the prevalence of dental caries among school children; to determine the association between of diet and dental caries. METHODS. The survey was conducted in the city of Chennai, India. A total of 200 children participated in the survey between the age groups of 13 to 14 years from private and public schools. In each of these age groups an attempt was made to include equal number of male and female subjects. Questionnaires were filled by the children and dental examination was done by dental surgeon with help of two assistants, which were recommended by WHO oral health assessment. Statically data was analyzed using the statistics packages spss17.0for windows. RESULTS. The diet pattern of Indian school going children aged between 13 to 14 years was not healthy among the boys and girls. Children under the poor socio-economic category consumed more healthy diet followed by the rich and average socio-economic group and consumption of unhealthy diet was more among the rich socio-economic group followed by average and poor socio-economic group. Children in the public school consumed healthy food more frequently than children from the private school (51.2% and 45.3% respectively). Prevalence... [to full text] / TIKSLAS. Šio tyrimo tikslas buvo nustatyti ryšį tarp dietos ir Dantų ėduonies tarp paauglių mokyklinio amžiaus vaikų, 13-14 metų amžiaus grupės miesto Chennai, Indija TIKSLAI. Sužinoti charakteringus mitybos ypatumus tarp mokyklinio amžiaus vaikų; analizuoti dantų ėduonies paplitimą tarp mokyklinio amžiaus vaikų; nustatyti ryšį tarp maitinimosi įpročių ir dantų ėduonies. METODAI. Tyrimas buvo atliktas Chennai mieste, Indija. Iš viso apklausoje dalyvavo 200 vaikų, nuo 13-14 metų amžiaus grupių iš privačių ir viešųjų mokyklų. Kiekvienoje iš šių amžiaus grupių buvo bandoma sudaryti vienodas skaičius vyrų ir moterų dalyvių. Anketos buvo užpildytos pačių vaikų ir odontologų. Apžiūrą atliko odontologas su dviem asistentais, kurie rėmėsi PSO burnos sveikatos vertinimo rekomendacijomis. Statiškai duomenys buvo analizuojami naudojant statistikos paketą spss17.0for windows. REZULTATAI. Dietos struktūra Indijos mokyklose, lankančių vaikų tarp 13 iki 14 metų, nebuvo sveika tarp berniukų ir mergaičių. Vaikai priklausantys prastai socialinei ekonominei kategorijai vartojo sveikesnę mitybą nei turtingose ir vidutinėse socialinėse ekonominėse grupėse. Vaikai nemokamoje valstybinėje mokykloje vartodavo sveiką maistą dažniau negu vaikai privačiose mokyklos (51.2 % ir 45.3 % atitinkamai). Dantų ėduonis paplitimas buvo daugiau tarp berniukų lyginant su mergaitėmis (54.0 % ir 46.0 %). Vaikai, priklausantys žemai socialinei ekonominei padėčiai, turėjo aukštesnį ėduonies paplitimą negu... [toliau žr. visą tekstą]

The use of mass spectrometry in the biosciences has undergone huge growth in re- cent years due to sustained effort in the development of new ionisation techniques, more powerful mass analysers and better bioinformatic tools. These developments mean that it is now possible to introduce complex crude biological-mixtures into a mass spectrometric platform and to obtain detailed information about the sample. The front-end sample handling techniques used for sample preparation have, for the most part, not changed despite the recent advances in hyphenation of liquid- chromatography and mass spectrometry required to tackle the issue of increased sample complexity. In this thesis the possibility of using Digital Microfluidics (DMF) for front-end sample preparation prior to mass-spectrometric analysis of protein samples has been investigated. DMF is a micro-electromechanical system (MEMS) technology used for manipulation of sub-microlitre droplets. The movement of discrete droplets of liquid is exploited using the Coulombic forces arising due to free charge polarisation. Droplets can be split, joined, dispensed and moved over a sub-surface electrode array. In this thesis a range of DMF devices have been designed, manufactured and coupled with mass spectrometric platforms for protein analysis. A variety of techniques for mass spectrometry- based analysis of biological samples from the fluidic chips have been investigated. A robotic system has been developed to automate sample introduction, manipulation and removal. Finally the application of on-chip sample purification and enzymatic digestion have been demonstrated, providing proof of concept for digital microfluidic sample preparation in mass spectrometry-based proteomics.

Understanding how defense signaling pathways regulate neuronal protection in the compromised central nervous system (CNS) is essential for combating neurodegenerative disorders. This is apparent in the intrinsic activation of the transcription factor Nrf2 during periods of oxidative stress, a hallmark of neurodegeneration. This regulator of the antioxidant response induces the transcription of genes essential for protecting against oxidative stress-induced damage and is a prime target for drug discovery. Delayed-release dimethyl fumarate (DMF), currently approved for the treatment of relapsing-remitting forms of multiple sclerosis (MS), is believed to mediate its effect via the Nrf2 pathway; however, the exact mechanisms of action are unknown. The primary aim of the studies outlined in this dissertation was to identify the molecular mechanisms of Nrf2 regulation and subsequent cellular protection conferred by DMF and its bioactive metabolite, monomethyl fumarate (MMF). For this thesis study, transcriptional profiling studies following oral administration of DMF were conducted to characterize DMF pharmacodynamic responses in the central nervous system (CNS) and peripheral tissues to understand the functional effects of DMF in vivo as well as explore the necessity of Nrf2 in this process. Data from these studies confirm earlier findings that DMF activates transcription of Nrf2 target genes in the CNS and periphery; however, tissue-specific gene expression was also observed, indicating additional levels of transcriptional control beyond Nrf2 activation. These findings suggest that there may be unique cytoprotective and immunomodulatory capabilities of DMF within specific tissues. In the CNS, a novel Nrf2 transcriptional target gene OSGIN1 was identified to be significantly upregulated following DMF treatment in vivo; however, the contribution of this gene to the pharmacodynamic properties of DMF or MMF has not been previously described. Therefore, the in vitro effects of MMF on OSGIN1 expression were characterized, and the necessity of OSGIN1 in mediating cytoprotective effects against toxic oxidative stress in human astrocytes was evaluated. These data identify a potential mechanism for MMF-mediated cytoprotection in human astrocytes that function in an OSGIN1 and p53-dependent manner. Overall, the experiments described in this dissertation allow for a broader understanding of endogenous cellular protection and how it can be used to combat CNS disorders.

No description available.

Orientador: Marcelo de Castro Meneghim / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-15T05:51:22Z (GMT). No. of bitstreams: 1 Tonello_AlineSampieri_D.pdf: 1951799 bytes, checksum: 27b4d5ce5f510d6096bf44b78d9cfbd9 (MD5) Previous issue date: 2010 / Resumo: O presente trabalho, composto por dois estudos teve como objetivos: a) descrever as prevalências de cárie e fluorose dentária em escolares de 12 anos de idade de Piracicaba/SP e Iracemápolis/SP, Brasil, 2007 e comparar estes dados com outros levantamentos epidemiológicos realizados anteriormente, em Piracicaba para a cárie dentária (1971-2005) e para a fluorose dentária (1991-2001) e em Iracemápolis para a cárie e fluorose dentária (1991-2004); b) avaliar a experiência de cárie e a prevalência de fluorose dentária em escolares de 12 anos de idade de Piracicaba/SP, Brasil, 2007 e verificar a relação entre essas alterações e os fatores socioeconômicos e variáveis comportamentais relacionadas com a saúde bucal. A amostra constituiu de 724 escolares provenientes de escolas públicas e privadas de Piracicaba/SP e 197 escolares provenientes de escolas públicas de Iracemápolis/SP. Os exames foram realizados por um examinador previamente calibrado, no pátio das escolas, sob luz natural, com auxílio de espelho bucal plano, sonda IPC e escovação prévia, seguindo as recomendações da OMS. A cárie dentária foi avaliada utilizando os índices CPOD e SiC (Significant Caries Index), e a fluorose pelo índice T-F. As variáveis socioeconômicas (renda familiar, número de residentes na mesma casa, escolaridade do pai e da mãe e habitação) e as informações comportamentais foram obtidas por meio de um questionário semi estruturado enviado aos pais. A associação entre as variáveis dependentes (CPOD e fluorose) e as variáveis independentes (variáveis socioeconômicas e comportamentais) foi determinada por meio de análise de regressão logística múltilpa. O CPOD médio foi de 0,85 (dp=1,54) e 1,02 (dp=1,61) e o índice SiC de 2,52 (dp=1,72) e 2,83 (dp=1,60) em Piracicaba e Iracemápolis, respectivamente. A prevalência de fluorose foi de 29,4% e 25,4% em Piracicaba e Iracemápolis, respectivamente. Comparando o presente estudo com outros levantamentos realizados anteriormente, foi observada uma significativa redução da experiência de cárie em ambas as cidades, (90,12%) em Piracicaba e 85,07% em Iracemápolis (p<0,01). Com relação à prevalência da fluorose dentária, verificou-se um aumento de 44,1% em Piracicaba, observando uma menor prevalência entre alguns anos. Já em Iracemápolis, o aumento foi de 1170%. Com base no modelo de regressão logística múltipla, as crianças pertencentes a uma família com renda mensal inferior ou igual a quatro salários mínimos apresentaram 2,58 vezes mais chances de terem cárie (CPOD>0) do que aquelas pertencentes a uma família com renda mensal superior a quatro salários mínimos. Além disso, aquelas crianças que consultaram ao dentista apresentaram 4,27 vezes mais chances de serem diagnosticadas ou tratadas em relação à cárie do que aquelas que nunca foram ao dentista. Porém, em relação à fluorose, o modelo de regressão logística não foi significativo. Em conclusão, os resultados desta tese demonstraram uma contínua redução da experiência de cárie em ambas as cidades no decorrer dos anos. Em relação à fluorose dentária, verificou-se uma tendência de estabilização em Piracicaba, porém em Iracemápolis, observou-se um aumento constante. Adicionalmente, observou-se uma associação entre a presença de cárie e renda familiar mensal, como também entre cárie e consultas ao dentista. Entretanto, em relação à fluorose dentária, nenhuma das variáveis testadas foi associada com esta alteração / Abstract: The present study was composed by two papers which aims were: a) to describe the prevalence of dental caries and dental fluorosis in 12-years-old schoolchildren from Piracicaba/SP and Iracemápolis/SP, Brazil, 2007 and to compare current prevalence rates with those from previous epidemiological surveys developed in Piracicaba, for dental caries (1971-2005), and for dental fluorosis (1991-2001) and in Iracemápolis for dental caries and dental fluorosis (1991-2004); b) to evaluate the caries experience and dental fluorosis prevalence in 12-years-old schoolchildren from Piracicaba/SP, Brazil, 2007 and to verify the relationship between these trends, socioeconomic factors, and behavioral variables related to oral health. The sample consisted of 724 schoolchildren from public and private schools from Piracicaba/SP and 197 schoolchildren from public schools from Iracemápolis/SP. Examinations were executed by a previously calibrated examiner, in outdoor settings, under natural light, using dental mirror, CPI probe, and previous tooth brushing, followed the WHO recommendations. Dental caries was measured by DMFT and SiC (Significant Caries Index) indexes and dental fluorosis by T-F index. Socioeconomic variables (monthly income, number of residents in home, mother's and father's education and home ownership) and behavioral information were collected by means of a parental semi structured questionnaire. Then multiple logistic regression analyses using the stepwise procedure were performed in order to verify the relationship among the dependent variables (DMFT and fluorosis) and the independent variables (socioeconomic and behavioral variables). The mean of DMFT was 0.85 (SD=1.54) and 1.02 (SD=1.61) and the SiC Index was 2.52 (SD=1.72) and 2.83 (SD=1.60) in Piracicaba and Iracemápolis, respectively. The fluorosis prevalence was 29.4% and 25.4% in Piracicaba and Iracemápolis, respectively. Comparing the present study to another previous epidemiological surveys, in both towns, a significant caries reduction of 90.12% (Piracicaba) and of 85.07% (Iracemápolis) (p<0.01), have been observed. Concerning dental fluorosis prevalence, an increase of 44.1% was noted in Piracicaba, where a small prevalence was observed throughout the years. Although, the increase was 1170% in Iracemápolis. By means of multiple logistic regression model, children, whose family earned up to four minimum wages, were 2.58 more prone to have caries (DMFT>0) than those whose family earned over four minimum wages. Besides, those children who visited the dentist were 4.27 more prone of being diagnosed with dental caries and receive treatment in comparison to those who never had visited the dentist. However, for fluorosis prevalence the multiple logistic regression model was not significant. In conclusion, the results of this thesis demonstrated continuous decrease in dental caries experience in both Brazilian towns. Concerning dental fluorosis, stabilization trends were observed in Piracicaba. In Iracemápolis, however, a constant increase was noted. Moreover, significant associations between the presence of dental caries and monthly family income, as well as between dental caries and visiting to the dentist, were observed. However, in relation to dental fluorosis, no tested variable was associated with the disturb / Doutorado / Saude Coletiva / Doutor em Odontologia

Made available in DSpace on 2017-07-24T19:22:28Z (GMT). No. of bitstreams: 1 Flavia Tanaka.pdf: 954441 bytes, checksum: 2a9a2dd535c25850feca5625419095e5 (MD5) Previous issue date: 2004-07-06 / In order to contribute to the epidemiological surveillance process and indeed to diagnose the real condition of oral health of public school children in the city of Maringá, Parana State, Brazil, it was conducted this research. The prevalence of dental caries was evaluate using systematic random sample which was constituted by 610 subjects, both genders, living in the urban cluster.The methodology was based on the SB Brazil Project – Conditions of Buccal Health of the Brazilian Population. It was used diagnosis criteria of the World Health Organization (WHO), which included permanent and deciduous teeth. The Kappa Test indicated concordance in two levels (good and very good) inter and intra examiners. The mean dmf-t and DMF-T indexes were 1,8 and 1,5, respectively. The decay mean present in decayed deciduous teeth higher (1,0) than in filled tooth (0,7), while referring to permanent teeth, the filled teeth presented higher (1,1) than the decayed teeth (0,4). The lost component “M” was not significant in both ages and revealed a positive tendency of the odontological services in maintaining permanent and deciduous teeth. At 6 years, 47,7% of children (30,8% dmf-t index of 1 to 3, 17,3% of 4 to 7 and 4,3% higher numbers) and at 12 years, 50% of children (33,2% with DMF-T index of 1 to 3, 21,6% of 4 to 7 and 2,1 higher numbers) were caries free. When the polarization phenomenon was confirmed, 21,6% and 23,7% of school children concentrated major rates of disease. At 12 years, the children presented a statistically significant difference (p<0.05) among genders, where boys presented superior DMF-T index. The comparison of the dental decay distribution between municipal and state schools did not identified statistically significant differences in the dmf-t index, but on the contrary, this differences were observed to DMF-T index .The comparison of the results in this study with epidemiological researches made in the same city in previous years (1991 and 1994), showed an effective reduction in the decay prevalence to 12 years children, although at 6 years old this same prevalence was not observed. It was concluded the oral health of school children of this evaluated sample is in accordance to the objectives proposed by the World Health Organization to the year of 2000, presenting low prevalence of decay and the continuity of the educational and prevention programs is essential to reach the projected goals to the year of 2010. / Visando contribuir com o processo de vigilância epidemiológica e conhecer as reais condições de saúde bucal de escolares de ensino público do município de Maringá, Paraná, Brasil, conduziu-se esta pesquisa. A prevalência de cárie foi avaliada em uma amostra sistemática constituída de 610 crianças de 6 e 12 anos de idade, de ambos os sexos, residentes na zona urbana. A metodologia empregada baseou-se no Projeto SB Brasil – Condições de Saúde Bucal da População Brasileira, incluindo-se a avaliação clínica de dentes decíduos e permanentes de acordo com os critérios de diagnóstico preconizados pela Organização Mundial da Saúde (OMS). O teste Kappa indicou boa e ótima concordância inter e intra examinadores. O ceo-d e o CPO-D médio foram iguais a, respectivamente, 1,8 e 1,5. Nos dentes decíduos, observou-se que a média de dentes decíduos cariados (1,0) predominou sobre a de obturados (0,7), ao passo que nos dentes permanentes, a média do componente obturado (1,1) predominou sobre a do cariado (0,4). A participação pouco expressiva do componente perdido “P”, nas duas idades revelou uma tendência positiva dos serviços odontológicos quanto à preservação de ambas dentições. Encontravam-se livres de cárie, aos 6 anos de idade, 47,7% das crianças (30,8% com ceo-d de 1 a 3, 17,3% de 4 a 7 e 4,3% com valores maiores) e aos 12 anos, 50,0% (33,2% com CPO-D de 1 a 3, 21,6% de 4 a 7 e 2,1% com valores maiores). Confirmando o fenômeno da polarização, 21,6% e 23,7% dos escolares concentraram as maiores taxas da doença. Aos 12 anos, houve diferença estatisticamente significante (p<0,05) na ocorrência de cárie entre os sexos, sendo esta superior para os meninos. Comparando-se a distribuição da cárie dentária entre escolas municipais e estaduais não foram identificadas diferenças estatisticamente significantes ao se analisar o índice ceo-d, porém estas foram observadas focando-se o índice CPO-D. A comparação dos resultados deste estudo com os obtidos em levantamentos epidemiológicos realizados no mesmo município, em anos anteriores (1991 e 1994), mostrou uma efetiva redução na prevalência de cárie para a idade de 12 anos, contudo, aos 6 anos esta mesma tendência não pôde ser observada. Conclui-se que a saúde bucal dos escolares pertencentes à amostra analisada está de acordo com as metas preconizadas pela OMS para o ano 2000, havendo o registro de baixa prevalência da doença cárie e que a continuidade de programas educativos e preventivos são fundamentais para se alcançar os novos objetivos propostos para o ano 2010.

The nasal mucosa provides a rapid, noninvasive route for drug administration to the systemic circulation and even potentially to the CNS. Nanoparticles made from the biodegradable polymer, PLGA, are of great interest for use in drug delivery systems due to PLGA’s relative safety and ease of surface modification. Nanoparticles may provide improved targeting and transport through the nasal mucosa. However, the optimal nanoparticle sizes and surface properties for intranasal delivery are unknown. In this study, we prepared PLGA nanoparticles within a size range of 50-70 nm containing the lipophilic fluorescent dye, Nile Red, using a surfactant-free nanoprecipitation method. The resulting nanoparticles were evaluated using dynamic light scattering and scanning electron microscopy. Nanoparticle uptake into the nasal mucosa was determined by exposing the tissues to nanoparticle dispersions for 30 or 60 minutes. The in vitro uptake of the nanoparticles by the nasal mucosal tissues revealed that the Nile Red-loaded PLGA nanoparticles were transported across the epithelial layer and accumulated in the sub-mucosal connective tissues. Nanoparticle uptake in the full thickness tissues was time dependent where 2% of the total loads of nanoparticles exposed to the tissues were measured in the mucosal tissue after 30 minutes and 4% were present in the tissues after 60 minutes. The rapid and measurable transfer of PLGA nanoparticles into the nasal mucosal tissues indicate that they may be an efficient delivery vehicle for drugs with either local or systemic activities.