Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-09-08T13:51:47Z No. of bitstreams: 1 OtavioHenriqueB.PintoDissertacao2017.pdf: 3191289 bytes, checksum: b7b005d77190f465fe0685851ff5f090 (MD5) / Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-09-08T13:52:01Z (GMT) No. of bitstreams: 1 OtavioHenriqueB.PintoDissertacao2017.pdf: 3191289 bytes, checksum: b7b005d77190f465fe0685851ff5f090 (MD5) / Made available in DSpace on 2017-09-08T13:52:02Z (GMT). No. of bitstreams: 1 OtavioHenriqueB.PintoDissertacao2017.pdf: 3191289 bytes, checksum: b7b005d77190f465fe0685851ff5f090 (MD5) Previous issue date: 2017-03-29 / Acidobacteria is one of the most abundant phyla in soil; however, it is not yet known the reasons for their success in terrestrial habitats. This characteristic may be due to mechanisms of resistance as well as the large amount of glycosyl hydrolases and transferases present in Acidobacteria. Therefore, this study aimed to investigate mechanisms of resistance that may contribute to the abundance of Acidobacteria in soil. In addition, the degradation of cellulose was investigated, to understand its participation in processes of plant biomass degradation. In this study, a member of subdivision 1 of Acidobacteria, Occallatibacter savannae AB23 was used as model. This isolate was taxonomically characterized and cellulose degradation and carotenoid production were evaluated. The degradation of cellulose was evaluated using carboxymethylcellulose (CMC). The pigments were identified since this bacterium produced several carotenoids under different environmental stimuli. The results showed that the isolate AB23 can assimilate CMC, however in a very slow way, requiring 2 months for the visualization of colonies and 1 year to visualization of biomass in liquid medium. For this isolate, endoglucanases were found associated with the cell fraction of culture. In addition, cellobiose has been shown to be a repressor and lactose an inducer of endoglucanases expression. It was possible to observe the production of carotenoids in AB23. By mass spectrometry, it was possible to find ions related to carotenoids: ?? -carotene, neurosporene, lycopene, ??-carotene, ??-carotene, phytoene and phytofluene. These carotenoids might be used as a resistance mechanism since they are produced in response to stress caused by light. However, the presence of vitamins, oxygen and trace elements are essential for carotenoid production. The characteristics found in this work may be one of the mechanisms of persistence and / or resistance that confer abundance for Acidobacteria in soil. / Acidobacteria ?? um dos filos mais abundantes no solo, entretanto, n??o se conhece as raz??es que levam a essa abund??ncia. Estudos indicam que essa caracter??stica pode ser devido a mecanismos de resist??ncia e a grande quantidade de glicosil hidrolases e transferases presentes em Acidobacteria. Assim, esse estudo teve como objetivo investigar mecanismos de resist??ncia que podem contribuir para a abund??ncia de Acidobacteria no solo. Al??m disso, foi investigada a degrada????o de celulose em Acidobacteria, procurando entender sua participa????o em processos de degrada????o de material originado de plantas. Neste estudo foi utilizado como modelo Occallatibacter savannae AB23. Este isolado foi caracterizado taxonomicamente e foram avaliadas a degrada????o de celulose e produ????o de caroten??ides. A degrada????o de celulose foi avaliada utilizando-se carboximetilcelulose (CMC). Para os pigmentos, foram identificados quais carotenoides essa bact??ria produz e os est??mulos que levam a sua produ????o. Os resultados mostraram que o isolado AB23 consegue assimilar CMC, no entanto de uma forma muito lenta, sendo necess??rios 2 meses para a visualiza????o de col??nias. Para este isolado, as endoglucanasases foram encontradas associadas ?? fra????o celular da cultura. Al??m disso, celobiose mostrou ser um repressor e a lactose um indutor da express??o de endocglucanases. Foi poss??vel observar a produ????o de pigmentos em AB23. Por espectrometria de massa, foi poss??vel encontrar ??ons relacionados aos carotenoides: ??-caroteno, neurosporeno, licopeno, ??-caroteno, ??-caroteno, fitoeno e fitoflueno. Estes devem estar sendo empregados como um mecanismo de resist??ncia, uma vez que eles s??o produzidos em resposta ao estresse causado pela luz. No entanto a presen??a de vitaminas, oxig??nio e elementos tra??o s??o essenciais para produ????o desses pigmentos. As caracter??sticas encontradas neste trabalho podem ser um dos mecanismos de persist??ncia e/ou resist??ncia que conferem abund??ncia para Acidobacteria no solo.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-11-20T12:50:15Z No. of bitstreams: 2 karoline_guedes_araujo.pdf: 416699 bytes, checksum: 717f931edff84a22776fa1c422d70315 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-11-20T12:50:36Z (GMT) No. of bitstreams: 2 karoline_guedes_araujo.pdf: 416699 bytes, checksum: 717f931edff84a22776fa1c422d70315 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-11-20T12:50:37Z (GMT). No. of bitstreams: 2 karoline_guedes_araujo.pdf: 416699 bytes, checksum: 717f931edff84a22776fa1c422d70315 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2011 / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / Objetivou-se com o desenvolvimento deste trabalho avaliar a produtividade de seis h?bridos de milho, o valor nutricional e as caracter?sticas fermentativas de suas silagens. Avaliou-se tamb?m qualidade das fibras destas silagens por meio das cin?ticas de tr?nsito e de degrada??o ruminal. Foram utilizados quatro animais fistulados no r?men, sendo o delineamento utilizado um quadrado latino (4x4), onde os tratamentos foram as fibras mordantadas de quatro h?bridos selecionados dentre os seis. Para o estudo de degradabilidade, as amostras mo?das em peneiras de 4 mm foram incubadas no r?men nos tempos 0, 6, 18, 48 e 96 horas, em saquinhos de n?ilon. A partir dos resultados encontrados, pode-se sugerir que os h?bridos estudados apresentam caracter?sticas nutricionais e produtivas adequadas para obten??o de silagens de qualidade. E que o h?brido DKB 390 apresentou melhor performance com rela??o a qualidade da fibra. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT The objective of this study was to evaluate the productivity of six corn hybrids, the nutritional and fermentation characteristics of their silage. There has also been evaluated the quality of these silages fibers through the transit kinetics and ruminal degradation. Four animals were fistuled in the rumen, and the design used a Latin square design (4x4), in which the treatments were the mordant fibers from four of the six selected hybrids. To study the degradability of the samples, samples grounded in 4mm sieves were incubated in the rumen at 0, 6, 18, 48 and 96 hours, in nylon bags. From these results, it can be suggest that studied hybrids have nutritional and productive measures to obtain silage quality. And the hybrid DKB 390, showed better performance with respect to fiber quality.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-06T16:33:02Z No. of bitstreams: 2 aline_nogueira_alves_santiago.pdf: 3992386 bytes, checksum: b8ec3c32c9f964b34275b2319b6b3edf (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-07T11:54:26Z (GMT) No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) aline_nogueira_alves_santiago.pdf: 3992386 bytes, checksum: b8ec3c32c9f964b34275b2319b6b3edf (MD5) / Made available in DSpace on 2016-01-07T11:54:31Z (GMT). No. of bitstreams: 2 license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) aline_nogueira_alves_santiago.pdf: 3992386 bytes, checksum: b8ec3c32c9f964b34275b2319b6b3edf (MD5) Previous issue date: 2015 / Um anodo perme?vel a fluido constitu?do por filme fino de di?xido de estanho dopado com antim?nio (Sb-SnO2) foi preparado por decomposi??o t?rmica sobre suporte de tela de a?o ASTM 316 e utilizado em reator eletroqu?mico do tipo filtro-prensa. O filme e o p? do ?xido foram investigados pelas t?cnicas de energia dispersiva de raios-X (EDX), difratometria de raios-X (DRX) e microscopia eletr?nica de varredura (MEV). O estudo do EDX indicou uma raz?o efetiva Sb/Sn maior que a nominal devido a volatiliza??o do Sn. O DRX revelou que o SnO2 est? presente na estrutura rutila, n?o sendo verificado a presen?a de picos relacionados ao ?xido de antim?nio devido sua baixa concentra??o. O estudo de MEV revelou a forma??o de filme compacto e n?o poroso, com presen?a de cristalitos superficiais. Para a caracteriza??o eletroqu?mica do anodo Sb-SnO2 foi realizado estudo de voltametria c?clica, sendo obtido a partir da curva voltam?trica uma baixa densidade superficial de carga, ou seja, o eletrodo ? de morfologia compacta. A curva de polariza??o revelou que o sobrepotencial para a rea??o de desprendimento de oxig?nio (RDO) ? de aproximadamente 1,75 V/ECS. O anodo Sb-SnO2 alojado numa c?lula filtro-prensa na condi??o de ?zero-gap? foi utilizado na degrada??o eletroqu?mica individual dos f?rmacos Diclofenaco S?dico e Paracetamol (Acetominofeno) na total aus?ncia de eletr?litos l?quidos. Os fatores que influenciam a taxa de degrada??o, tais como a densidade de corrente aplicada (5?25 mA/cm2) e a concentra??o inicial dos f?rmacos (30?70 mg/L) foram avaliados. A degrada??o dos f?rmacos seguiu a cin?tica de pseudo-primeira ordem, sendo monitorada por an?lise da demanda qu?mica de oxig?nio (DQO), cromatografia l?quida de alta efici?ncia (CLAE) e espectros na regi?o do ultravioleta-vis?vel (Uv/Vis). Foram verificadas taxas de degrada??es superiores a 80% ap?s 3,5 h de rea??o com a gera??o de subprodutos. O grau de instabilidade do eletrodo foi avaliado em fun??o do seu tempo de uso. O anodo Sb-SnO2 apresentou uma boa efic?cia para a remo??o dos f?rmacos Diclofenaco S?dico e Paracetamol (Acetominofeno) dissolvidos em ?guas, mas com um curto tempo de vida ?til. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT A fluid-permeable thin film anode composed by tin dioxide doped with antimony (Sb-SnO2) was prepared by thermal decomposition onto a ASTM 316 stainless steel screen support, and applied in a filter-press electrochemical reactor. The film and the oxide powder were investigated by the energy dispersive x-ray (EDX), X-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques. The EDX study revealed an effective Sb/Sn ratio higher than the nominal one due to the volatilization of Sn. The XRD revealed that SnO2 is present as a rutila structure, not being verified the occurrence of peaks related to the antimony oxide due its low concentration. SEM analysis showed the formation of compact and non-porous film, with the presence of surface crystallites. For the electrochemical characterization of the Sb-SnO2, it was carried out a cyclic voltammetry study, being obtained from the voltammetric curve a low surface charge density, i.e., the electrode has a compact morphology. The polarization curve showed that the overpotential for the oxygen evolution reaction (OER) is about 1.75 V/SCE. The Sb-SnO2 anode housed in a filter-press cell in the zero-gap condition was applied on the electrochemical degradation of the Sodium Diclofenac and Paracetamol (acetominophen) drugs in the total absence of liquid electrolytes. Factors affecting the degradation rate, such as the applied current density (5?25 mA/cm2) and the initial concentration of drugs (30?70 mg/L) were evaluated. The degradation of drugs followed the pseudo first-order kinetics, being monitored by analysis of the chemical oxygen demand (COD), high performance liquid chromatography (HPLC) and spectra in the ultraviolet-visible (UV/Vis) region. It was verified degradation rates higher than 80% after 3.5 h of reaction with the formation of byproducts. The degree of electrode instability was evaluated as a function of its use time. The Sb-SnO2 anode exhibited a good efficiency for the removal of the drugs Diclofenac and Paracetamol (acetominophen) dissolved in water, but with a short service life.

Made available in DSpace on 2016-04-28T14:59:44Z (GMT). No. of bitstreams: 1 2007- Vinicius Pimentel Silva.pdf: 2345431 bytes, checksum: 39b89841a5352bbec854edc58a64e8ff (MD5) Previous issue date: 2007-02-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This work aimed to evaluate digestibility and degradability of nutrients, and kinetics of passage in horses fed with forages using mobile bag and in situ cecal digestion. Two digestion trials were carried out and seven forages were used, lucerne (Medicago sativa), peanut (Arachis pintoi), Desmodium ovalifolium, Stylosanthes guianensis, Cajanus cajan, Macroptyloma axilares and coastcross (Cynodon dactylon cv. coastcross). Basal diet were composed by 80% hay and 20% concentrate. In first assay with mobile bags, five crossbreed horses were used with age varying from 17 to 27 years and average weight of 350 kg. A nylon cloth were used with 45? pore size, and bags with dimensions of 7,5 x 2 cm, containing 510mg DM of sample/bag. These assay had 12 days of duration, and three for adaptation, five days for naso-gastric insertion of bags and four days for bags collection in feces. Randomized block design were used with seven treatments and five repetitions. Naso-gastric insertion of bags were made twice a day, at morning and afternoon, inserting 29 bags, and four bags of each feed and one blank. Transit time and excretion of bags were evaluated until 96h from insertion. Nutrients digestibility of forages were calculated through residues inside the bags. In situ assay was conducted with caecum fistulated horse, weight 210Kg. Bags with 20x 6,5cm were used with 5,2g of sample incubated inside caecum along times 0, 2, 4, 6, 8, 12, 24, and 48 hours. A randomized design was used with seven treatments and three repetitions during 30 days. Orskov & McDonald degradation model was used to fit the nutrients degradation curves. Nutrients digestibility of peanut, Desmodium and Macroptiloma were high than others roughages (P<0.05). Any difference were observed (P>0.05) among transit time, mediun retention time and passage rate, average values were 23.34, 48.63 and 2.07, respectively. Degradation rate were high to peanut, Desmodium and Macroptyloma. Peanut fiber and protein degradability is superior to others tropical legumes, and peanut presented high protein contents. Tropically forages peanut, Stylosanthes guianensis and Macroptyloma axilares presented potential to be use in horse diets. / Este trabalho foi realizado com o objetivo de estimar a digestibilidade e a degrada??o dos nutrientes e a cin?tica de passagem de alimentos volumosos em eq?inos utilizando as t?cnicas de sacos de n?ilon m?veis e da digest?o in situ. Foram realizados dois ensaios de digest?o e os alimentos volumosos utilizados nos dois estudos foram, alfafa (Medicago sativa), amendoim forrageiro (Arachis pintoi), Desmodium ovalifolium, Stylosanthes guianensis, guandu (Cajanus cajan), Macroptyloma axilares e capim coastcross (Cynodon dactylon cv. coastcross). A alimenta??o foi uma dieta basal composta por 80% de feno e 20% de concentrado. No primeiro ensaio, com sacos m?veis, foram utilizados cinco eq?inos mesti?os com idades variando de 17 a 27 anos, com peso vivo m?dio de 350kg. Para a confec??o dos sacos, utilizou-se tecido de n?ilon com porosidade de 45? com dimens?es de 7,5x 2cm, inserindo 510mg de MS de amostra/saco. O ensaio teve dura??o de 12 dias, sendo tr?s para a adapta??o, cinco para inser??o g?strica dos sacos e quatro de coleta dos sacos. O delineamento foi em blocos inteiramente casualizados com sete tratamentos e cinco repeti??es. A sonda nasog?strica foi inserida duas vezes ao dia, de manh? e ? tarde com 29 sacos, sendo quatro para cada alimento e um em branco, anotou-se o tempo de tr?nsito e a excre??o dos sacos foi considerada at? 96 horas ap?s a inser??o. A estimativa da digestibilidade dos nutrientes dos alimentos volumosos foi calculada atrav?s do res?duo obtido no saco. No ensaio in situ utilizou-se um animal fistulado no ceco, com peso vivo de 210 Kg e sacos de 20 x 6,5 cm, com 5,2 g de amostra, incubadas no ceco nos tempos de 0, 2, 4, 6, 8, 12, 24 e 48 horas. Utilizou-se o delineamento inteiramente casualizado com sete tratamentos e tr?s repeti??es, com dura??o de 30 dias e o modelo de degrada??o citado por Orskov & McDonald para descrever as curvas de degrada??o dos nutrientes. A digestibilidade dos nutrientes dos alimentos volumosos Amendoim, Desmodium e Macroptyloma foram maiores (P<0,05). N?o houve diferen?a (P>0,05) entre os alimentos em rela??o ao tempo de tr?nsito, tempo m?dio de reten??o e taxa de passagem dos sacos, observando-se os valores m?dios de 23,3 h, 48,6 h e 2,0 h, respectivamente. Os valores da taxa de degrada??o foram superiores no Amendoim, Desmodium e Macroptiloma. A degrada??o da fibra do amendoim forrageiro ? superior ?s demais leguminosas tropicais, assim como seu conte?do prot?ico. Os alimentos volumosos tropicais amendoim, Stylosanthes guianensis e Macroptyloma axilares apresentaram potencial para o uso em dietas para eq?inos.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-08-16T19:30:56Z No. of bitstreams: 1 2014 - Carla da Silva Teba.pdf: 4808769 bytes, checksum: c0f54c8ea9fb94f4469d54eb3764c138 (MD5) / Made available in DSpace on 2017-08-16T19:30:56Z (GMT). No. of bitstreams: 1 2014 - Carla da Silva Teba.pdf: 4808769 bytes, checksum: c0f54c8ea9fb94f4469d54eb3764c138 (MD5) Previous issue date: 2014-09-02 / CAPES / Whey is one the most polluting by-products of the food industry and their perishability does not allow storage for long periods, then it's necessary to find appropriate destination. The whey protein have potential anticarcinogenic, activities hypocholesterolemic, anti-inflammatory, immunomodulatory action, among others. The rice consists mainly of starch, and this structure contributes to the production of snacks high technological quality. The objective of this study was develop and characterize mixed extruded flour of rice and whey protein concentrate aiming use in the manufacture nutritional and practical products. The parameters used in the processing of thermoplastic extrusion were: formulation (% whey protein concentrate), moisture content and temperature of the last heating zone. The extrudates were subjected to physical characterization and functional technology assessment. The highest rates of growth were observed for the tests with up to 4 % whey protein concentrates in their formulations and were processed with low humidity generally less than 20 % and milder temperatures, generally at or below 140 ?C. Through the visual characterization of extruded and the micrographs it was observed that the samples processed with low moisture content (16.64 and 18%) were more homogeneous cells. The X-ray diffraction showed that the profile of the crystal samples is characteristic of type "A" due to the presence of crystalline domains in its structure. The flours viscosity profile is consistent with the characteristics of pre- gelatinized products, being dissolved without needing to cook. The pre-gel flour with blends of rice and whey protein concentrate present viscous, homogeneous and with low capacity of retrogradation. The addiction protein concentrate in rice flour increased the ash and proteins, indicating that the mineral and amino acid composition of the mixed flours may also have a nutritional improvement. Generally, the processing conditions do not significantly affect the nutritional value of the flours blends. The acceptance test performed with the cookies prepared with the mixed flours 8, 10 and 16 (6, 7.36 and 4 % whey protein concentrate, respectively) indicated that the biscuit with a higher content of protein concentrate showed better overall evaluation, taste and purchase intent. The porridges were produced with pre-gelatinized flour containing 2, 4 and 7.36% whey protein concentrate and sensory analysis evaluated the attributes appearance, flavor and consistency, plus purchase intent. The results showed that for most of the evaluated attributes and purchase intent, the sample with higher content of protein concentrate showed the best results. The porridges were produced with pre-gelatinized flour containing 2, 4 and 7.36% whey protein concentrate and sensory analysis evaluated the attributes appearance, flavor and consistency, plus purchase intent. The results showed that for most of the evaluated attributes and purchase intent, the sample with higher content of protein concentrate showed the best results. We conclude that it is possible to produce pre-gel flours containing rice and whey protein concentrate with good nutritional, microbiological and technological features for the production of cookies and porridges rapid dissolution. / O soro de leite ? um dos subprodutos mais poluentes da ind?stria de alimentos e sua natureza perec?vel n?o permite a estocagem por per?odo prolongado, sendo necess?rio encontrar destino adequado aos volumes produzidos. Atribuem-se ?s prote?nas do soro de leite poss?veis atividades anticarcinog?nica, hipocolesterol?mica, anti-inflamat?ria, a??o imunomoduladora, entre outras. O arroz ? constitu?do principalmente por amido, e devido a sua estrutura, contribui para a produ??o de snacks de elevada qualidade tecnol?gica. O objetivo desse trabalho ? desenvolver e caracterizar farinhas mistas pr?-gelatinizadas de arroz e concentrado proteico de soro de leite bovino, obtidas por extrus?o termopl?stica, visando utiliza??o na elabora??o de produtos nutritivos e pr?ticos. Foram consideradas como vari?veis independentes: formula??o (% de concentrado proteico de soro de leite na mistura com farinha de arroz), umidade da mistura da farinha no processamento e temperatura da ?ltima zona de aquecimento. Os extrudados elaborados foram submetidos ? caracteriza??o f?sica e avalia??o tecnol?gica funcional. Os melhores ?ndices de expans?o foram observados para os ensaios com at? 4 % de concentrado proteico e que foram processados com baixa umidade e temperaturas mais brandas. Atrav?s da caracteriza??o visual dos extrudados e das micrografias foi poss?vel observar que as amostras processadas com baixo conte?do de umidade (16,64 % e 18 %) apresentaram c?lulas mais homog?neas. Por meio da difratometria de raios X verificou-se que o perfil das amostras ? caracter?stico ao cristal do tipo "A", devido ? presen?a de dom?nios cristalinos em sua estrutura. As farinhas mistas extrudadas se destacaram por possuir elevada viscosidade a frio, baixa viscosidade a quente (95?C) e baixo poder de retrograda??o. Os resultados observados nos par?metros de viscosidade avaliados mostram correla??o destas propriedades com as caracter?sticas de f?cil reconstitui??o e boa solubiliza??o em meio aquoso, sem a necessidade de cozimento. A incorpora??o de concentrado proteico na farinha de arroz promoveu incremento no teor de cinzas e prote?nas, indicando melhoria na composi??o mineral e de amino?cidos das farinhas mistas. De modo geral, as condi??es de processamento utilizadas n?o afetaram de forma significativa o valor nutricional da maior parte das farinhas mistas produzidas. O teste de aceita??o realizado com os biscoitos elaborados com as farinhas mistas referentes aos ensaios 8, 10 e 16 (6, 7,36 e 4 % de concentrado proteico, respectivamente) indicou que o biscoito com maior teor de concentrado proteico apresentou melhor avalia??o global, sabor e inten??o de compra. Os mingaus foram produzidos com farinhas pr?-gelatinizadas contendo 2, 4 e 7,36 % de concentrado proteico e submetidos ? an?lise sensorial. Para a maior parte dos atributos avaliados e para a inten??o de compra, a amostra com maior teor de concentrado proteico apresentou os melhores resultados. Conclui-se que ? poss?vel produzir farinhas mistas pr?-gelatinizadas de arroz e concentrado proteico de soro de leite bovino com boas caracter?sticas nutricionais, microbiol?gicas e tecnol?gicas tanto para a elabora??o de biscoitos quanto para a produ??o de mingaus de r?pida dissolu??o.

Made available in DSpace on 2014-12-17T15:54:59Z (GMT). No. of bitstreams: 1 JosePGB_DISSERT.pdf: 1710204 bytes, checksum: 5ee486fb6a3193951f164bedc0f67abc (MD5) Previous issue date: 2012-02-09 / Universidade Federal do Rio Grande do Norte / The semi-arid Northeast region are coming through changes since its formation causing changes in the landscape and increased degradation. The economic dynamics which was established at the Potiguar semiarid had for a long time settled in livestock, subsistence agriculture and cotton, however, these economies decline from the year 1970. Thus, the Potiguar spaces are undergoing by a process of space reordering marked by the growth of agricultural activities and the process of urbanization. In the Potiguar western region these changes weaken significantly transform the landscape. In these areas, the process of urbanization and agricultural activities have caused changes in the hinterland landscape changing the natural dynamics of the caatinga biome that has been subject to negative interference with the indiscriminate removal of vegetation. In this case, the specific study area of the watershed of Cajazeiras Creek located in the cities of Pau dos Ferros, ?gua Nova, Encanto and Rafael Fernandes, changes in the landscape has led to degradation and environmental fragility. From this perspective, the research objective is to assess the dynamics of the landscape of the watershed of Cajazeiras Creek, semiarid region of the state of Rio Grande do Norte, identifying the degrading factors, the most degraded areas and/or susceptible to degradation and environmental fragility. The research investigated the phenomena of light geosystems theory that emphasizes the integration of elements in shaping the landscape. During this reporting period, it was carried out exploratory and bibliographical research, and geoprocessing techniques to assess the social and environmental conditions of the researched area and its environmental fragility. To do so, first, it was made an multitemporal analisis on the basis of the years 1984, 1996 and 2009 demonstrating that the dynamics of use and land cover in the watershed of Cajazeiras Creek showed a significant change in the landscape, especially in the caatinga vegetation. This has been recovering in various areas of the watershed, especially in its upper course in the mountains of Bom Sera, in Encanto city. The analysis showed the need to develop policies and programs for the maintenance, restoration and preservation of the caatinga vegetation of the river network and of water bodies. In addition, it was identified the most degraded areas of the watershed, environmental fragility and potential emerging that take into account the landscape components such as climate, topography, geology, pedology, geomorphology and the use and occupation of the soil in an integrated manner. The results show that the landscape of the watershed has medium and high environmental fragility throughout its area, especially on the banks of the river network provided by the use and occupation of the soil in an inadequate way with the environmental conditions of the watershed / O semi?rido nordestino vem passando por mudan?as desde sua forma??o acarretando transforma??es na paisagem e aumento da degrada??o. A din?mica econ?mica que se estabeleceu no semi?rido potiguar durante muito tempo esteve assentada na pecu?ria, agricultura de subsist?ncia e algod?o, entretanto, essas economias declinam a partir dos anos de 1970. Desse modo, os espa?os potiguares est?o passando por um processo de reordenamento espacial marcado pelo crescimento das atividades agropecu?rias e pelo processo de urbaniza??o. Na regi?o do oeste potiguar tais mudan?as fragilizam e transformam significativamente a paisagem. Nessas ?reas, o processo de urbaniza??o e a atividade agropecu?ria t?m provocado mudan?as na paisagem sertaneja alterando a din?mica natural do Bioma Caatinga que passou a sofrer interfer?ncias negativas com a retirada indiscriminada da vegeta??o. No caso, espec?fico da ?rea em estudo a microbacia do Riacho Cajazeiras localizada nos munic?pios de Pau dos Ferros, ?gua Nova, Encanto e Rafael Fernandes as altera??es na paisagem tem provocado a degrada??o e a fragilidade ambiental. Nessa perspectiva, o objetivo da pesquisa consiste em avaliar a din?mica da paisagem da microbacia do Riacho Cajazeiras, semi?rido potiguar, identificando os fatores degradantes, ?s ?reas mais degradadas e/ou suscept?veis a degrada??o e as fragilidades ambientais. A pesquisa investigou os fen?menos a luz da teoria geossist?mica que enfatiza a integra??o dos elementos na forma??o da paisagem. Para a realiza??o do trabalho foram realizadas as pesquisa explorat?ria, bibliogr?fica, o trabalho de campo e t?cnicas de geoprocessamento para avaliar as condi??es socioambientais da ?rea de pesquisa e sua fragilidade ambiental. Para tanto, primeiramente, foi feito uma analise multitemporal com base nos anos de 1984, 1996 e 2009 demonstrando que a din?micade do uso e da ocupa??o do solo na microbacia do Riacho Cajazeiras mostrou uma mudan?a significativa na paisagem, especialmente, na vegeta??o de caatinga. Esta vem se recuperando em varias areas da microbacia, principalmente, em seu alto curso nas Serras do Bom Sera, do Encanto. A an?lise mostrou a necessidade de desenvolver pol?ticas e programas voltados para a manuten??o, recupera??o e preserva??o da vegeta??o de caatinga da rede fluvial e dos corpos d ?gua. Al?m disso, foram identificadas as ?reas mais degradadas da microbacia e a fragilidade ambiental potencial e emergente que levam em considera??o os componentes da paisagem tais como: clima, relevo, geologia, pedologia, geomorfologia e uso e ocupa??o do solo de forma integrada. Os resultados mostram que a paisagem da microbacia apresenta fragilidade ambiental m?dia e alta em toda sua ?rea, destacando-se, nas margens da rede fluvial proporcionada pelo o uso e ocupa??o do solo de forma inadequada com as condi??es ambientais da microbacia

Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-07-15T15:08:13Z No. of bitstreams: 1 paula_alves_oliveira.pdf: 1583436 bytes, checksum: 38dccdbd3bbed8d6245bca3ca279be63 (MD5) / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: on 2016-07-15T20:04:24Z (GMT) / Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-07-15T20:12:13Z No. of bitstreams: 1 paula_alves_oliveira.pdf: 1583436 bytes, checksum: 38dccdbd3bbed8d6245bca3ca279be63 (MD5) / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: rever: Refer?ncia ABNT Tipo: opem Acess Nota de disserta??o/Tese: colocar ano Palavra Chave: cada uma em um campo, sem pontua??o Ag?ncia Financiadora: Sempre por extenso e com a abrevia??o entre par?nteses no final ABSTRACT: manter o termo antes do resumo em ingl?s. Inserir t?tulo em ingl?s. on 2016-07-18T14:45:06Z (GMT) / Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-07-19T12:39:36Z No. of bitstreams: 1 paula_alves_oliveira.pdf: 1583436 bytes, checksum: 38dccdbd3bbed8d6245bca3ca279be63 (MD5) / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: Refer?ncia ABNT Tipo Nota de disserta??o/Tese Palavra Chave Ag?ncia Financiadora Refer?ncia ABNT Tipo Nota de disserta??o/Tese Resumos Palavra Chave Ag?ncia Financiadora Exemplo: http://acervo.ufvjm.edu.br/jspui/handle/1/559 on 2016-07-21T15:23:46Z (GMT) / Submitted by Alexandre Soares (alexandredesoares@yahoo.com.br) on 2016-08-25T11:47:51Z No. of bitstreams: 1 paula_alves_oliveira.pdf: 1583436 bytes, checksum: 38dccdbd3bbed8d6245bca3ca279be63 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-08-25T12:47:19Z (GMT) No. of bitstreams: 1 paula_alves_oliveira.pdf: 1583436 bytes, checksum: 38dccdbd3bbed8d6245bca3ca279be63 (MD5) / Made available in DSpace on 2016-08-25T12:47:19Z (GMT). No. of bitstreams: 1 paula_alves_oliveira.pdf: 1583436 bytes, checksum: 38dccdbd3bbed8d6245bca3ca279be63 (MD5) Previous issue date: 2015 / Anglo American / O objetivo deste trabalho foi ampliar os estudos sobre as esp?cies existentes em ?reas de campos rupestres mineradas, em ?reas a serem mineradas ou no entorno da minera??o, a fim de dar suporte aos programas de recupera??o desses ecossistemas degradados. A disserta??o foi estruturada em dois artigos. No primeiro avaliou-se a cobertura do solo por esp?cies do estrato herb?ceo-arbustivo em ?rea de campo rupestre quartz?tico em diferentes n?veis de conserva??o, em Diamantina, Minas Gerais. A comunidade foi estratificada de acordo com a intensidade de dist?rbios em cada local (estrato). A amostragem da vegeta??o foi feita por meio do m?todo da interse??o na linha. Nesse ambiente, a comunidade estudada apresentou elevada diversidade flor?stica, com o registro de 99 esp?cies, distribu?das em 49 g?neros e 22 fam?lias. As fam?lias Poaceae e Cyperaceae foram as mais importantes na coloniza??o do ambiente. Dentre as classes analisadas, aquelas com maior cobertura relativa foram, no total, solo exposto (11,30 %), afloramento de rocha (9,59 %) e mat?ria morta (9,02 %). Pela classifica??o de TWINSPAN, algumas esp?cies apresentaram diferentes comportamentos de acordo com o estrato em que se localizavam, por?m elas estiveram presentes em todos os estratos, mostrando que s?o capazes de colonizar os diferentes ambientes dentro da ?rea de estudo, o que leva a crer que poder?o ser utilizadas na coloniza??o de ambientes degradados semelhantes. No segundo artigo, procurou-se caracterizar as comunidades herb?ceo-subarbustivas sobre campo rupestre quartz?tico (CRQ) e ferruginoso (CRF) em Concei??o do Mato Dentro, MG, em diferentes per?odos do ano, quanto ? similaridade e ? estrutura fitossociol?gica, al?m de indicar esp?cies nativas com potencial para uso em programas de restaura??o de ?reas degradadas semelhantes. Foram registradas, no total, 17 fam?lias bot?nicas, sendo oito no campo rupestre ferruginoso e 15 no campo rupestre quartz?tico. As fam?lias com maior n?mero de esp?cies foram Fabaceae (3), Asteraceae (2) e Euphorbiaceae (2) no CRF e Poaceae (7), Asteraceae (6) e Melastomaceae (6) no CRQ. A comunidade estudada apresentou baixa similaridade e diversidade flor?stica, em ambos os locais e per?odos, n?o havendo diferen?as significativas entre os valores de H?, pelo teste t de Hutcheson. A DCA evidenciou a forma??o de dois ambientes distintos, separando o ambiente de CRF do CRQ. As esp?cies das fam?lias Eriocaulaceae e Xyridaceae ocorreram exclusivamente no ambiente sobre quartzito, sendo algumas desta ?ltima fam?lia classificadas como indicadoras desse ambiente, o que desperta interesse quando ao seu uso em programas de restaura??o de campos rupestres quartz?ticos. As esp?cies com maiores valores de import?ncia no CRF foram Bulbostylis fimbriata e Centrosema brasilianum e no CRQ Echinolaena inflexa, podendo ser indicadas para programas de restaura??o de ambientes semelhantes. / Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / The objective was to expand studies of the species in areas of mined campos rupestres, in areas to be mined or around mining in order to support the recovery of these degraded ecosystems programs. The dissertation was structured in two articles. In the first evaluated the soil cover by species of herbaceous and shrub layer in campo rupestre quartz?tico at different levels of conservation, in Diamantina, Minas Gerais. The community was stratified according to the intensity of disturbances at each site (stratum). The vegetation sampling was performed by the method of intersection on the line. In this environment, the community studied showed high floristic diversity, with the record of 99 species belonging to 49 genera and 22 families. The Poaceae and Cyperaceae were the most important in the colonization of the environment. Among the analyzed classes, those having a relative cover were, in total, bare soil (11.30%), rock outcrops (9.59%) and dead matter (9.02%). By TWINSPAN classification, some species showed different behaviors according to the stratum in which they were located, but they were present in all segments, showing that they are able to colonize different environments within the study area, which suggests that they may be used in the colonization of similar degraded environments. In the second article, we tried to characterize the herbaceous communities on campo rupestre quartz?tico (CRQ) and ferruginoso (CRF) in Concei??o do Mato Dentro, Brazil, in different periods of the year, as the similarity and vegetation structure, and indicates native species with potential for use in similar degraded areas restoration programs. They recorded a total of 17 botanical families, eight in campo rupestre ferruginoso and 15 in the campo rupestre quartz?tico. The families with the highest number of species were Fabaceae (3), Asteraceae (2) and Euphorbiaceae (2) in the CRF and Poaceae (7), Asteraceae (6) and Melastomaceae (6) in the CRQ. The community presented low similarity and floristic diversity in both places and periods, with no significant differences between the values of H ', the t test Hutcheson. The DCA showed the formation of two distinct environments, separating the CRF environmentally CRQ. The species of Eriocaulaceae and Xyridaceae families occurred exclusively in the environment of quartzite, some of the latter family classified as indicators of this environment, which arouses interest as to their use in campo rupestre quartz?tico restoration programs. The species with the highest values of importance in the CRF were Bulbostylis fimbriata and Centrosema brasilianum and CRQ Echinolaena inflexa and may be suitable for similar environments restoration programs.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2015-12-15T17:45:12Z No. of bitstreams: 1 SinaraCarlaDaSilvaAraujo_DISSERT.pdf: 1885448 bytes, checksum: b4a8226a4f1778e4f626da3394657f9a (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2015-12-29T20:51:37Z (GMT) No. of bitstreams: 1 SinaraCarlaDaSilvaAraujo_DISSERT.pdf: 1885448 bytes, checksum: b4a8226a4f1778e4f626da3394657f9a (MD5) / Made available in DSpace on 2015-12-29T20:51:37Z (GMT). No. of bitstreams: 1 SinaraCarlaDaSilvaAraujo_DISSERT.pdf: 1885448 bytes, checksum: b4a8226a4f1778e4f626da3394657f9a (MD5) Previous issue date: 2014-08-22 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Os microrganismos apresentam uma imensa diversidade gen?tica e est?o presentes em toda biosfera, no entanto cerca de 1% das esp?cies pode ser cultivada por t?cnicas laboratoriais padr?o. A metagen?mica tornou poss?vel o acesso direto ao genoma microbiano derivado de amostras ambientais, utilizando t?cnicas independentes de cultivo. A metodologia permite obter informa??o funcional de prote?nas, assim como a identifica??o de potenciais produtos com interesse biotecnol?gico e de novos recursos biol?gicos industrialmente explor?veis, a exemplo novas solu??es para impactos ambientais. ?reas contaminadas com petr?leo s?o caracterizadas por um grande ac?mulo de hidrocarbonetos e surfactantes s?o utilizados para sua biorremedia??o. Sendo assim, a abordagem metagen?nima foi utilizada para selecionar genes envolvidos no processo de degrada??o e biossurfacta??o de hidrocarbonetos. Em um trabalho anterior, o DNA ambiental (eDNA) foi extra?do de amostras de solo coletadas em duas diferentes ?reas (Caatinga e rio salino) do Rio Grande do Norte (Brasil), as bibliotecas metagen?micas foram constru?das e analisadas funcionalmente. Os clones capazes de degradar o ?leo foram avaliados quanto ? capacidade de sintetizar biossurfactante. O clone foi sequenciado e an?lise da sequencia revelou uma ORF com 897 pb, 298 amino?cidos e prote?na de peso molecular pr?ximo a 34 kDa. A busca por homologia no GenBank revelou similaridade com a sequ?ncia que codifica uma prote?na hipot?tica de representantes da fam?lia Halobacteriaceae, que foram mostradas recentemente como produtoras de biossurfactantes. A presen?a da sequ?ncia codificante inserida e do fen?tipo adquirido foram confirmadas. Primers foram desenhados e suas ORFs amplificadas por PCR. Em seguida, foram subclonadas em vetor de express?o pETDuet-1, contendo uma cauda de histidina, para express?o e posterior purifica??o da prote?na de interesse. Os testes foram realizados para confirma??o da atividade de biossurfactante e degrada??o de hidrocarbonetos e apresentaram resultados positivos. O ensaio de imunodetec??o (western blot) com a utiliza??o do anticorpo monoclonal Anti-His? confirmou a presen?a da prote?na ambiental. Esse estudo foi o primeiro a relatar uma poss?vel prote?na com atividade biossurfactante obtida a partir de uma abordagem metagen?mica. / The microorganisms have a vast genetic diversity and they are present throughout the biosphere, however, only about 1% of the species can be cultivated by traditional cultivation techniques. Within this diversity there is a huge pool genetic and biological being explored. The metagenomics has enabled direct access to microbial genome derived from environmental samples using independent methods of cultivation. The methodology enables to obtain functional information about the proteins, as well as identify potential products with biotechnological interest and new industrially exploitable biological resources, such as new solutions to environmental impacts. Oil-contaminated areas are characterized by a large accumulation of hydrocarbons and surfactants may be used for bioremediation. Thus, the metagenomic approach was used in this study in order to select genes involved in the degradation and hydrocarbon emulsification. In a previous work, the environmental DNA (eDNA) was extracted from soil samples collected from two different areas (Caatinga and Saline River) of Rio Grande do Norte (Brazil), the metagenomic libraries were constructed and functionally analyzed. The clone able to degrade the oil was evaluated for the ability to synthesize biosurfactants. The sequence analysis revealed an ORF with 897 bp, 298 amino acids and a protein with around 34 kDa. The search for homology in GenBank revealed sequence similarity with a hypothetical protein of representatives Halobacteriaceae family, who were recently shown as strains producing biosurfactants. The presence of the inserted coding sequence and the acquired phenotype was confirmed. Primers were designed and the ORF amplified by PCR. The ORF was subcloned into pETDuet-1 expression vector for subsequent purification of the protein of interest containing a histidine tail. The tests performed to confirm the biosurfactant activity and the ability of hydrocarbon degradation showed positive results. The immunodetection test (western blot) using the monoclonal AntiHis? confirmed the presence of the environmental protein. This study was the first to report a possible protein with biosurfactant activity obtained from a metagenomic approach

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-05-10T21:22:14Z No. of bitstreams: 1 AlanaRayzaVidalJeronimoDoNascimento_DISSERT.pdf: 2928827 bytes, checksum: d27ed974dc569fc8d3e33d0de625afc7 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-05-13T00:33:56Z (GMT) No. of bitstreams: 1 AlanaRayzaVidalJeronimoDoNascimento_DISSERT.pdf: 2928827 bytes, checksum: d27ed974dc569fc8d3e33d0de625afc7 (MD5) / Made available in DSpace on 2016-05-13T00:33:56Z (GMT). No. of bitstreams: 1 AlanaRayzaVidalJeronimoDoNascimento_DISSERT.pdf: 2928827 bytes, checksum: d27ed974dc569fc8d3e33d0de625afc7 (MD5) Previous issue date: 2015-09-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A minera??o promove altera??es significativas na paisagem e na qualidade do solo, al?m de ser considerada uma das principais fontes antr?picas de metais pesados no ambiente. A aus?ncia de medidas de prote??o sanit?ria e ambiental no controle dos impactos gerados contribui para o aumento do risco de degrada??o n?o apenas na ?rea de lavra, mas em toda a extens?o da ?rea de influ?ncia da minera??o. Neste contexto, diagn?sticos de qualidade do solo e dos res?duos da minera??o s?o imprescind?veis para orientar medidas que promovam a redu??o dos impactos e a recupera??o das ?reas degradadas. No munic?pio de Currais Novos, localizado na regi?o semi?rida do Estado do Rio Grande do Norte, algumas mineradoras realizam a explora??o da scheelita desde a d?cada de 1940. A mina Olho d??gua foi desativada em 1976 e a mina Barra Verde est? em atividade. Nessas minas, as atividades de lavra e de beneficiamento da scheelita geraram pilhas de est?ril e rejeito que est?o depositadas no solo sem qualquer medida de prote??o. Este trabalho teve como objetivo avaliar atributos f?sicos e qu?micos do solo e os teores de metais pesados nas minas Barra Verde e Olho d??gua a fim de identificar est?gios de degrada??o nas ?reas mineradas e estabelecer indicadores de qualidade que facilitem a recupera??o e monitoramento ambiental na regi?o. Os resultados demonstraram que a aus?ncia de medidas de controle e de recupera??o nas minas contribui para a intensifica??o dos processos erosivos, o que amplia o potencial de difus?o de contaminantes para os demais componentes da bacia hidrogr?fica. As ?reas de deposi??o de est?ril e rejeito na mina ativa apresentaram n?vel de degrada??o do solo mais acentuado dentre as ?reas mineradas. Na mina desativada, o crescimento espont?neo da vegeta??o tem favorecido a pedog?nese dos substratos remanescentes da minera??o e a recupera??o da qualidade do solo, indicando que a t?cnica de revegeta??o ? adequada para estabiliza??o e recupera??o das fun??es ecossist?micas das ?reas mineradas. Os teores de Cd, Cu e Pb acima dos valores de investiga??o estabelecidos pela legisla??o brasileira apontam para a necessidade de medidas de remedia??o nas ?reas mineradas. A sensibilidade na distin??o entre a ?rea natural e minerada dos atributos f?sforo dispon?vel, pH, acidez potencial, argila, nitrog?nio total, mat?ria org?nica, densidade do solo, porosidade total e densidade de part?culas e os metais Cd, Cu e Pb faz com que sejam considerados bons indicadores de qualidade do solo a serem utilizados em programas de recupera??o e monitoramento ambiental das ?reas mineradas. / Mining promotes significant changes in the landscape and soil quality, and is considered one of the major anthropogenic sources of heavy metals in the environment. The absence of measures of health and environmental protection in controlling the impacts generated contributes to the increased risk of degradation not only in the mined area, but in the full extent of the area affected by mining. In this context, soil quality and mining waste diagnostics are essential to guide measures that promote the reduction of impacts and the recovery of degraded areas. In the municipality of Currais Novos, located in the semi-arid region of the state of Rio Grande do Norte, some mining companies exploit the scheelite ore since 1940s. Olho d??gua mine was shut down in 1976 and Barra Verde mine is in operation. These mines, the mining and beneficiation activities of scheelite generated piles of tailings and overburden that are deposited in the soil without any protective measure. This study aimed to evaluate physical and chemical properties of soil and heavy metal content in Barra Verde and Olho d??gua mines to identify stages of degradation in mined areas and establish quality indicators for facilitate recovery and environmental monitoring in the region.The results showed that the absence of control and recovery measures in the mines contributes to the intensification of erosion, which increases the potential spread of contaminants to the other componentes of the watershed. The overburden deposition and tailings areas in the active mine showed stronger level of land degradation among the mined areas. In the disabled mine, the spontaneous growth of vegetation has favored the pedogenesis of the remaining substrates mining and recovery of soil quality, indicating that the revegetation technique is suitable for stabilization and recovery of ecosystem functions of mined areas. Cd, Cu and Pb beyond values of investigation established by Brazilian law indicate to need for remediation measures in mined areas. The sensitivity in distinguishing between the natural and mined area of available phosphorus, pH, potential acidity, clay, total nitrogen, organic matter, bulk density, total porosity, particle density Cd, Cu and Pb makes them good soil quality indicators to be used in recovery and environmental monitoring programs of mined areas.

Made available in DSpace on 2014-12-17T14:52:52Z (GMT). No. of bitstreams: 1 CarlosCGD.pdf: 1489111 bytes, checksum: 001f763e52671d83a36d43eff64141cc (MD5) Previous issue date: 2006-05-19 / The objective of this study was to investigate the environmental perception of: People with common-sense knowledge on the desertification process in RN. People with scientific knowledge on the desertification process in RN. Focal points in the combat at desertification of the RN and public ministery representant with actions in interinstitutional articulations promoter (and/or relative actions) at the desertification process in the RN. The research was carried in the city of Natal-RN and in two small cities of the Serid? region (RN): Caic? and Currais Novos. The research carried, is classified as exploratory and 22 people were interviewed. The research includes: The propension/intensity of the desertification in the RN and in the Serid? region; Evaluation of the knowledge of those interviewed, concerning the subject desertification ; Problems in order to combat desertification; Causes of desertification; The profile of the interviewed. The results of this present study indicate that the a desertification process is more agressive in the Serid? region than in the state of RN, being the two following: the absence of preocupation of the affected population with the process and the escarcity of governamental recurses, indicates how problems greather in the combat to the phenomen. Decreasing of produtivity in the agriculture and increasing of the migration to the urbans centers have been the main consequences of the process, that have at water scarcity, deforestation and extraction of argil (being this, regional factor), relevant variables in the influence to the surgiment of the desertification process of the RN / O objetivo deste trabalho foi investigar a percep??o ambiental de: Detentores de conhecimento popular acerca do processo de desertifica??o no RN. Detentores de conhecimento cient?fico acerca do processo de desertifica??o no RN. Pontos focais no combate ? desertifica??o do RN e representante do minist?rio p?blico que atuasse na promo??o de articula??o interinstitucional (e/ou a??es relacionadas) ao processo de desertifica??o no RN. A pesquisa foi realizada em Natal-RN e em dois munic?pios da regi?o do Serid? (RN): Caic? e Currais Novos. A pesquisa realizada, que ? enquadrada como explorat?ria, contou com 22 pesquisados e abordou: A propens?o/intensidade da desertifica??o no RN e no Serid?; Avalia??o do conhecimento do pesquisado, acerca do assunto desertifica??o ; Problemas no combate ? desertifica??o; Causas da desertifica??o; Perfil dos pesquisados. Os resultados obtidos apontam que a desertifica??o ? mais grave na regi?o do Serid? que no Estado como um todo, sendo a falta de preocupa??o da popula??o afetada pelo processo e a escassez de recursos governamentais, indicadas como principais problemas no combate ao fen?meno. Perda de produtividade agr?cola e eleva??o da migra??o para os centros urbanos evidenciaram-se como as principais conseq??ncias do processo, que tem na escassez de ?gua, no desmatamento e na retirada de argila (sendo esse ?ltimo, fator regional), vari?veis de destacada import?ncia na influ?ncia ao surgimento do processo de desertifica??o do RN