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This thesis is an examination of the sex and gender differences in measures of relative deprivation for Winnipeg, Manitoba, and the value of these measures to predict health outcomes. Within theoretical frameworks of relative deprivation and intersectionality, principal component analysis was used to test nineteen different versions of a national area-based deprivation index using Census variables, for the total population and for males and females separately. Only one version of the deprivation index provided consistent factor scores, in keeping with the theoretical constructs, for the total, female-only and male-only populations for Winnipeg. Administrative health data were used to calculate area-level rates of select health outcomes and binomial negative regressions were then used to analyze whether the “best” index was predictive of health outcomes for the three populations. In regression models, only the “material” component of the deprivation index was predictive of the health outcomes, but results varied across the three populations. The application of the “best” deprivation index to health planning may depend on the health issue and the population in question. This thesis confirmed that examining the intersections of sex, gender and deprivation in population health research unmasks important differences that would otherwise be missed and could have implications in health planning.

South Africa's water law dispensation has changed dramatically with the promulgation of the National Water Act 36 of 1998. The previous distinction between public and private water has been abolished and the Minister of Water Affairs and Forestry has been appointed to act as trustee of the nation's water resources. Through the working of section 4(4), exclusive rights of water use, which were in force before 1998, were replaced by water allowances, granted in the discretion of the relevant authority. The key issue, which is investigated in this article, is whether the state, through the provisions of the National Water Act, expropriated vested rights in property or whether such infringement merely constituted a deprivation. The new concept of property in terms of section 25 of the Constitution of the Republic of South Africa and the distinction between deprivation and expropriation are examined. It is indicated that the concept of property in South African law has been extended to include not only ownership but also rights in property. Although no definition of property has been formulated in the Constitution, it appears from applicable authority, that this development in the law of property is supported by the Constitution and that the protection granted by the property clause will stretch as far as the inclusion of rights in property. It is for this reason that the existing water use rights, which were available to certain individuals in terms of the 1956 Water Act, can be classified as property. Section 25(1) authorises the infringement of private property in certain defined instances. Despite the many academic works which define the difference between deprivation and expropriation as described in section 25(2), the Constitutional Court clarified this matter in First National Bank of SA Ltd Va Wesbank v Commissioner for the South African Revenue Services 2002 7 BCLR 702 (CC). Expropriation is described as a sub-category of deprivation. Section 25(1) must thus be used as the starting point in all cases in which an investigation is conducted into the constitutional validity of an infringement of property. Only when it has been established that the requirements of section 25(1) have been complied with, is the question of whether deprivation constitutes expropriation, asked. The requirements for deprivation, expropriation and inverse condemnation are discussed with reference to applicable case law. After the aim of the National Water Act was weighed up against the disadvantages which individuals suffer through the infringement of their vested rights, the conclusion was reached that the nation's need for sustainable water resources carries more weight than the individual's exclusive right of use of water. A constitutionally valid deprivation has thus occurred. Due to the fact that the state did not appropriate any rights in this process, the conclusion was reached that this provision does not amount to an expropriation. It does however appear that the provisions of the National Water Act can give rise to inverse condemnation or constructive expropriation in specific circumstances. / Thesis (LL.M. (Estate Law))--Potchefstroom University for Christian Higher Education, 2003.

Objectives: Men who receive androgen deprivation therapy (ADT) for prostate cancer (PCa) are at risk of several adverse effects that can be detrimental to both their physical and mental health. Common adverse effects include weight gain, muscle wasting, cardiovascular morbidity, fatigue and impaired quality of life (QOL). This study tested whether a combined aerobic and resistance exercise program can alleviate some of these symptoms in men receiving ADT. Design: Men with PCa, aged 50-80 years, receiving ADT were recruited to participate in this prospective randomized controlled trial. Subjects were assigned to a usual care group (UCG) or an exercise intervention group (EIG). The EIG completed a 16 week combined aerobic and resistance exercise program. Outcomes measures were assessed at baseline, 16 weeks, and 24 weeks and included: cardio-respiratory fitness; muscle strength and endurance; body composition; and reports of QOL, fatigue, mood, partner relations, and exercise behaviour. Results: Fifteen men were recruited to this study, but two participants in the EIG did not finish the study leaving the EIG with an n = 6 and the UCG with an n = 7. The exercise program did not lead to changes in weight, BMI or body fat. There was a small, close to significant, increase in muscle mass in the EIG over the intervention period (p = 0.052). This is encouraging as it demonstrates that exercise can counteract the catabolic effects of ADT. Interestingly, cardio-respiratory fitness improved over the course of the study for both groups. Muscular fitness, however, improved only for the EIG. There was a significant difference in chest press strength (p = 0.041) and leg press strength was bordering significance (p = 0.058). Unexpectedly, QOL declined for both groups during the intervention (p = 0.029). Participants in both groups also reported increased levels of fatigue from baseline to 24 weeks, although these changes were not significant (p = 0.586). Mood worsened over the study period for both groups from baseline to 16 weeks, but this increase in anxiety and depression was reduced at the follow-up period. These changes, too, were not significant (p = 0.364). Reports of partner relationships trended towards lower scores from baseline to 16 weeks. The men’s report in both groups and the women’s report in the EIG improved at the 24 week mark, but women in the UCG experienced further decline. Surprisingly, participants in both groups reported increases in exercise behaviour from baseline to 24 weeks. This could account for the lack of difference found in many of the measures. The power of this study was 0.22. Conclusion: Although this was a small study, it showed that a combined aerobic and resistance exercise program can have some positive benefits for men with PCa who are receiving ADT. Larger trials are needed to further examine the role of exercise in ameliorating the side effects of ADT, particularly in the areas of mood and partner relationships.

This thesis examines the influence of nutritional phosphate (Pi) deprivation on extracellular proteins secreted by the model plant Arabidopsis thaliana. Initial studies compared the secretome of Pi-sufficient (+Pi) versus Pi-deficient (-Pi) Arabidopsis cell cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationship between mRNA levels and relative amounts of selected secretome proteins was assessed. The results indicate that transcriptional control is but one of many factors contributing to Arabidopsis Pi starvation responses and highlight the importance of parallel biochemical and proteomic studies of –Pi plants. Three purple acid phosphatase (APase) isoforms were fully purified from the culture media of –Pi Arabidopsis cells and identified as AtPAP12 (At2g27190) and two AtPAP26 (At5g34850) glycoforms. As each purple APase exhibited broad substrate specificities and pH-activity profiles, it is hypothesized that their combined activities facilitate Pi scavenging from soil-localized organophosphates during nutritional Pi deprivation. AtPAP26 is dual-targeted during Pi stress since an earlier report demonstrated that it is also the principal intracellular (vacuolar) APase upregulated by -Pi Arabidopsis. The results indicate that differential glycosylation influences AtPAP26’s substrate specificity and subcellular targeting. An atpap26 T-DNA insertional mutant lacking AtPAP26 transcripts and immunoreactive AtPAP26 polypeptides exhibited: (i) 9- and 5-fold lower shoot and root APase activity, respectively, which did not change in response to Pi starvation, (ii) a 40% reduction in secreted APase activity during Pi deprivation, (iii) 35 and 50% reductions in free and total Pi concentration, respectively, in shoots of –Pi plants, and (iv) impaired shoot and root development when subjected to Pi deficiency. By contrast, no deleterious influence of AtPAP26 loss of function was apparent in +Pi plants. The results establish a firm role for AtPAP26 in the acclimation of Arabidopsis to Pi deficiency. The identification and functional characterization of secreted proteins upregulated by –Pi Arabidopsis is relevant to applied efforts to engineer Pi-efficient transgenic plants, needed to minimize the input of expensive, unsustainable, and polluting Pi fertilizers in crop production. / Thesis (Ph.D, Biology) -- Queen's University, 2010-04-28 17:20:46.892

This thesis is an examination of the sex and gender differences in measures of relative deprivation for Winnipeg, Manitoba, and the value of these measures to predict health outcomes. Within theoretical frameworks of relative deprivation and intersectionality, principal component analysis was used to test nineteen different versions of a national area-based deprivation index using Census variables, for the total population and for males and females separately. Only one version of the deprivation index provided consistent factor scores, in keeping with the theoretical constructs, for the total, female-only and male-only populations for Winnipeg. Administrative health data were used to calculate area-level rates of select health outcomes and binomial negative regressions were then used to analyze whether the “best” index was predictive of health outcomes for the three populations. In regression models, only the “material” component of the deprivation index was predictive of the health outcomes, but results varied across the three populations. The application of the “best” deprivation index to health planning may depend on the health issue and the population in question. This thesis confirmed that examining the intersections of sex, gender and deprivation in population health research unmasks important differences that would otherwise be missed and could have implications in health planning.

Transient Receptor Potential Melastatin 7 (TRPM7) is a ubiquitously expressed divalent cation channel implicated as a key regulator of neuronal cell death in stroke. Our research group has previously shown that TRPM7 dependent cytoskeletal regulation particularly via cofilin mediates neuronal death in oxygen glucose deprivation (in vitro stroke model). LIMK1 phosphorylation was also shown to decrease downstream of TRPM7 activation during anoxia. In the present study we investigated the effects of TRPM7 activation during anoxia, on three regulators of LIMK and cofilin; Rho-associated kinase 2 (ROCK2), P-21 activated kinase 3 (PAK3) and Slingshot family phosphatase 1 (SSH1). Our findings suggest that PAK3 activity is downregulated during OGD through TRPM7 independent mechanisms. However, SSH1 activity appears to be regulated downstream of TRPM7 in a manner that is consistent with LIMK and cofilin regulation. Overall, our work suggests that SSH1 is a new link between anoxia-induced TRPM7activity and cofilin hyperactivation.

Transient Receptor Potential Melastatin 7 (TRPM7) is a ubiquitously expressed divalent cation channel implicated as a key regulator of neuronal cell death in stroke. Our research group has previously shown that TRPM7 dependent cytoskeletal regulation particularly via cofilin mediates neuronal death in oxygen glucose deprivation (in vitro stroke model). LIMK1 phosphorylation was also shown to decrease downstream of TRPM7 activation during anoxia. In the present study we investigated the effects of TRPM7 activation during anoxia, on three regulators of LIMK and cofilin; Rho-associated kinase 2 (ROCK2), P-21 activated kinase 3 (PAK3) and Slingshot family phosphatase 1 (SSH1). Our findings suggest that PAK3 activity is downregulated during OGD through TRPM7 independent mechanisms. However, SSH1 activity appears to be regulated downstream of TRPM7 in a manner that is consistent with LIMK and cofilin regulation. Overall, our work suggests that SSH1 is a new link between anoxia-induced TRPM7activity and cofilin hyperactivation.

Bakgrund: Sömn är ett mänskligt grundläggande behov och bör därför tillgodoses hos patienter som vårdas på sjukhus. Patienter som vårdas på intensivvårdsavdelningar upplever dock ofta upprepade avbrott på sömnen och sömnbrist vilket kan leda till både fysiska och psykiska negativa konsekvenser. Intensivvårdsmiljön med dess oljud, starka belysning och frekventa vårdrelaterade interaktioner leder ofta till oförmåga hos patienterna att få en adekvat sömn. Syfte: Att belysa olika interventioner sjuksköterskan kan tillämpa för att förbättra sovmiljön och sömnkvaliteten hos patienter som vårdas på en intensivvårdsavdelning. Metod: En litteraturöversikt med grund i analys av kvantitativ forskning. Resultat: I resultatet framkom sju interventioner som delades in i tre olika kategorier: skapa en bättre sovmiljö, avskärmning från ljud och ljus samt förbereda patienten för sömn. De sju interventionerna som identifierades var: tysta/störningsfria perioder, riktlinjer/ramverk innefattande aktiviteter för att kontrollera yttre störande faktorer, utbildning, öronproppar och ögonmask, adderat "white noise", musik samt akupressur. Slutsats: Genom att tillämpa en eller fler av de sju interventionerna kan sovmiljön och sömnkvalitet förbättras för patienter som vårdas på intensivvårdsavdelningar. Dock krävs ytterligare forskning inom området då vissa interventioner ger motstridiga resultat. Klinisk betydelse: Sömn är ett nödvändigt behov och har en stor betydelse för återhämtning från sjukdom och bör därför tillgodoses av sjuksköterskan. Resultatet i denna litteraturöversikt kan ge en ökad förståelse för vilka interventioner sjuksköterskan kan tillämpa i sitt omvårdnadsarbete för att förbättra sovmiljön och sömnkvalitet för patienter som vårdas på intensivvårdsavdelningar. / Background: Sleep is a basic human need and should be addressed in patients being treated in hospital. Patients cared for in intensive care units often experience repeated interruptions of sleep and sleep deprivation, which can lead to both physical and psychological adverse consequences. The intensive care environment with its noise, strong lighting and frequent care-related interaction often leads to the inability of patients to get adequate sleep. Aim: To illustrate the various interventions the nurse can implement to improve the sleep environment and quality of sleep in patients who are cared for in an intensive care unit. Methods: A literature review with its basis in an analysis of quantitative research. Results: The result emerged in seven interventions that were divided into three different categories: creating a better sleep environment, shielding from light and sound, and preparing the patient for sleep. The seven interventions identified were: quiet/non-disturbance periods, guidelines/framework including activities to control disturbing environmental factors, education, ear plugs and eye mask, added "white noise", music and acupressure. Conclusion: By applying one or more of the seven interventions the sleep environment and sleep quality for patients cared for in intensive care units may improve. However, as some interventions produced conflicting results, further research in the area is required. Clinical significance: Sleep is an essential requirement and has great importance in the recovery from illness and should therefore be carefully understood by the nurse. Findings from the literature review can provide a better understanding of which interventions nurses can apply in their work to improve the sleep environment and sleep quality for patients cared for in intensive care units.

South Africa's water law dispensation has changed dramatically with the promulgation of the National Water Act 36 of 1998. The previous distinction between public and private water has been abolished and the Minister of Water Affairs and Forestry has been appointed to act as trustee of the nation's water resources. Through the working of section 4(4), exclusive rights of water use, which were in force before 1998, were replaced by water allowances, granted in the discretion of the relevant authority. The key issue, which is investigated in this article, is whether the state, through the provisions of the National Water Act, expropriated vested rights in property or whether such infringement merely constituted a deprivation. The new concept of property in terms of section 25 of the Constitution of the Republic of South Africa and the distinction between deprivation and expropriation are examined. It is indicated that the concept of property in South African law has been extended to include not only ownership but also rights in property. Although no definition of property has been formulated in the Constitution, it appears from applicable authority, that this development in the law of property is supported by the Constitution and that the protection granted by the property clause will stretch as far as the inclusion of rights in property. It is for this reason that the existing water use rights, which were available to certain individuals in terms of the 1956 Water Act, can be classified as property. Section 25(1) authorises the infringement of private property in certain defined instances. Despite the many academic works which define the difference between deprivation and expropriation as described in section 25(2), the Constitutional Court clarified this matter in First National Bank of SA Ltd Va Wesbank v Commissioner for the South African Revenue Services 2002 7 BCLR 702 (CC). Expropriation is described as a sub-category of deprivation. Section 25(1) must thus be used as the starting point in all cases in which an investigation is conducted into the constitutional validity of an infringement of property. Only when it has been established that the requirements of section 25(1) have been complied with, is the question of whether deprivation constitutes expropriation, asked. The requirements for deprivation, expropriation and inverse condemnation are discussed with reference to applicable case law. After the aim of the National Water Act was weighed up against the disadvantages which individuals suffer through the infringement of their vested rights, the conclusion was reached that the nation's need for sustainable water resources carries more weight than the individual's exclusive right of use of water. A constitutionally valid deprivation has thus occurred. Due to the fact that the state did not appropriate any rights in this process, the conclusion was reached that this provision does not amount to an expropriation. It does however appear that the provisions of the National Water Act can give rise to inverse condemnation or constructive expropriation in specific circumstances. / Thesis (LL.M. (Estate Law))--Potchefstroom University for Christian Higher Education, 2003.