Mon travail de thèse s’est développé, pour le cas particulier des observations satellitales à partir de l’orbite géostationnaire. Le but général a été de caractériser la variabilité diurne des propriétés optiques inhérentes (i.e. les coefficients d‘atténuation et de rétrodiffusion particulaire, cp et bbp) et apparentes (i.e. la réflectance, R) des eaux océaniques (« forme » du cycle diurne, amplitude, variations saisonnières, causes). Ces propriétés optiques déterminent le signal qu’un instrument embarqué à bord d’un satellite peut observer. Cette étude est une étape préliminaire permettant de comprendre la physique du problème, avant de pouvoir évaluer la possibilité de quantifier cette variabilité diurne à partir des observations satellitaires. La variabilité diurne de cp et bbp est donc étudiée en fonction des saisons définies selon les conditions physico-trophiques: le mélange, le bloom, son déclin, et l’oligotrophie. cp et bbp sont caractérisés par un cycle diurne, augmentation le jour et diminution la nuit, confirmant les tendances générales connues. Ce phénomène se produit durant toutes les saisons avec une variabilité intra-saisonnière qui différencie les cycles de cp et bbp: cp montre une amplitude plus large durant le bloom, contrairement à bbp les minima de cp et bbp sont synchronisés tandis que les maxima de bbp sont atteints 3 à 6 h avant ceux de cp, sauf pendant le bloom. L’augmentation journalière de bbp se reflète dans les changements de R. Toutefois, les résultats ont montré que l’inversion ne permet pas de reproduire l’augmentation journalière de bbp de façon satisfaisante. Les techniques d’inversion existantes ne sont pas adaptées pour une utilisation à haute fréquence. / My thesis is developed for the particular case of satellites observations from geostationary orbit. The overall aim was to characterize the diel variability of water inherent (i.e. the particulate beam attenuation and backscattering coefficients, cp and bbp) and apparent (i.e. reflectance, R) optical properties (“shape”of diel cycle, amplitude, seasonal variations, origins). These properties determine the signal which is observed from sensor aboard satellite. This study is a preliminary step in understanding the physical problem, before assessing the possibility of quantifying the diel variability from satellite observations. Diel variability of cp and bbp is studied by season, as defined by physical and trophic conditions: mixing, bloom proliferation, its decline, and oligotrophy. Both cp and bbp are characterized by a diel cycle of daytime increase and nighttime decrease, confirming known general trends. This occurs across all seasons, but intra-seasonal variability differed between cp and bbp cycles: unlike bbp, cp show larger amplitude of diurnal variation during the bloom cp and bbp minima are synchronized while bbp minima are attained 3-6 h before those of cp, except during the bloom. Daily changes of R are consistent with the variations of bbp. Nevertheless, it is still difficult to reproduce the diurnal increase of bbp by inversion of R. The differences observed between in situ and modeled values of bbp suggest that the performance of inversion is degraded when it is used at high frequency.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-03-12T00:12:47Z No. of bitstreams: 1 Disserta??o - C?ssia Alves de S?.pdf: 408040 bytes, checksum: a24d9c29b600bca2f7903bdca077b81c (MD5) / Made available in DSpace on 2016-03-12T00:12:47Z (GMT). No. of bitstreams: 1 Disserta??o - C?ssia Alves de S?.pdf: 408040 bytes, checksum: a24d9c29b600bca2f7903bdca077b81c (MD5) Previous issue date: 2013-03-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The daily activity pattern of social wasps can be influenced by different mechanisms such as environmental restrictions or interspecific interactions. However, the importance of interspecific interactions on the determination of the daily activity patterns may decrease on habitats with high environmental complexity. In this sense, we evaluated two hypotheses in order to understand what factors affect the daily activity patterns of social wasps: 1) The abundance overlap of different species during the day is lower when environmental complexity is greater, and 2) Thermal restrictions may constrain wasp activity. We counted the abundance of several wasp species foraging throughout the day on extrafloral nectaries in two plantations of caupi bean: on esurrounded by a pasture matrix (low structural complexity) and the other surrounded by fruit trees (high structural complexity). Afterwards, we evaluated the abundance overlap during the day, the abundance variations per hour and the preferential time of start and end of day activity. Temporal overlapping between species during the day was high for both plantations. The majority of species had a bimodal abundance pattern, with one abundance peak during the morning and the other during the afternoon. Species of major size started their activity during later hours, while species of minor size were active on every hour. These results indicate two scenarios: first, competition seems to below in these communities and second, temperature seems to be particularly restrictive for the activity of species of greater size. / A coleta de recursos gluc?dios pelas vespas sociais ao longo do dia pode variar como resposta a diferentes mecanismos, podendo responder a restri??es ambientais ou a intera??es interespec?ficas. Por?m, a import?ncia relativa das intera??es interespec?ficas pode ser reduzida em sistemas com maior complexidade ambiental. Para compreender esse efeito, avaliamos as seguintes hip?teses: 1) A sobreposi??o nos hor?rios de forrageamento das esp?cies de vespas sociais ao longo do dia ? menor quando a complexidade ambiental ? maior e 2) o padr?o de atividade di?rio das vespas ao longo do dia ? determinado por restri??es ambientais. Para isso, quantificamos a abund?ncia de diferentes esp?cies de vespas sociais forrageando em nect?rios extraflorais em duas planta??es de do feij?o caupi: uma circundada por matriz de pasto (baixa complexidade estrutural) e outra por frut?feras (alta complexidade estrutural). Iniciamos as observa??es e coletas ao longo do dia, depois que os NEFs do feij?o estavam ativos, e avaliamos o padr?o de sobreposi??o temporal de abund?ncia, o padr?o de abund?ncia em cada hor?rio, bem como a rela??o entre o hor?rio de in?cio e fim de atividade e o tamanho de cada esp?cie. A sobreposi??o temporal foi alta para as duas ?reas. O padr?o de abund?ncia foi bimodal ao longo do dia. As esp?cies de maior tamanho entraram em atividade em hor?rios mais tardios enquanto as esp?cies de pequeno tamanho n?o apresentaram um padr?o de inicio e fim de atividade. Esses resultados indicam que a competi??o entre esp?cies de vespas sociais ? baixa, permitindo a alta sobreposi??o do hor?rio de atividade nas duas ?reas. J? a temperatura parece restringir o hor?rio de atividade para vespas de grande porte.

Spatial heterogeneity in ecosystem metabolism may play a critical role in determining ecosystem functions. Variation in ecosystem metabolism between macrophyte patches in shallow wetlands at the extremes of freshwater habitats has not been investigated. We estimated ecosystem metabolism in mesocosms containing different macrophytes using 24-hour oxygen curves to test our hypotheses: (1) net aquatic production (NAP) during spring and summer would be similar among algal patches (metaphyton and Chara), (2) NAP in algal patches would be greater than patches dominated by the vascular plant Potamogeton foliosus, (3) heterotrophy and anaerobiosis would be greatest in patches dominated by Lemna, and (4) the pond would be autotrophic in the spring and fall but heterotrophic in the summer. We found that different patches generated differences in NAP but not always as we predicted. NAP was different among algal patches in the spring and summer, and only metaphyton was more heterotrophic than P. foliosus. In the summer Chara and Lemna patches were heterotrophic and metaphyton became autotrophic. As predicted, the pond was net autotrophic in the spring and heterotrophic in the summer with an absence of patchiness in fall attributed to the dominance of Lemna. This research suggests the importance of macrophyte patchiness in wetlands in determining patterns of ecosystem metabolism despite challenges in measuring 24 hour oxygen curves (e.g. oxygen supersaturation). Consequently, macrophyte traits may be important in determining spatial heterogeneity of ecosystem metabolism in shallow ponds.

State and Federal agencies have created sanctuaries and speed zones to help reduce manatee mortality while incorporating the recreational and commercial resource needs of these same habitats for humans. Specific habitat resources are considered necessary to increase manatee survivorship. We have only recently begun to address how manatees use some of these resources based on physiological or reproductive strategies. In this study, I quantified patterns of habitat use during seasonal and diel periods for different sex and reproductive manatee classes using data from a radio-telemetry study conducted by the Florida Fish and Wildlife Conservation Commission during 1991-1996. I used five environmental geographic data layers: bathymetry, distance to seagrass, distance to shoreline, distance to warm water refuge sites, and distance to fresh water sources, to discriminate seasonal and diel habitat use patterns for different manatee classes: males (M), females with calves (FWC), and females without calves (FNC). Mean occupancy values were calculated for environmental variable locations and seasonal, diel, and manatee class differences were tested using a Multi-Response Permutation Procedure (MRPP). Nonmetric Multidimensional Scaling (NMS) was used to visualize the ordination patterns of the manatee classes and to assess importance of correlated environmental variables. Significant differences in habitat use were noted between summer and winter based on distances to warm water, seagrass, and fresh water sources but similar habitat use patterns were exhibited within summer diel periods among manatee classes. All manatee classes appeared to have used a higher proportion of locations closer in proximity to seagrass at night than day in winter indicating a disproportionate difference in feeding bouts between diel periods. These differences may be attributed to adjusting feeding strategies to reduce thermoregulatory costs or to decrease human interactions. Differences in patterns were exhibited for the winter diel periods specifically for the FWC manatee classes during winter days. FWC had a higher proportion of locations within the warm water refuges during the day indicating a possible trade off situation between food consumption and thermal exposure. This study demonstrates coarse and fine scale patterns of variation in habitat use for manatees both seasonally and daily within winter. It also suggests that during winter months, manatees were not just utilizing their habitat but they appeared to have preferences and selection for certain habitat types. Recovery of a species is greatly enhanced when patterns of habitat use within the species' environment has been clearly defined. Understanding more specifically what types of habitats manatees choose might allow management to adjust strategies for protection of key habitats while encouraging further recovery of this species.

Processes during the meroplanktonic phase regulate population dynamics for many marine benthic invertebrates. I examined changes in vertical distribution of different meroplanktonic larvae in a coastal embayment during a stable period, at high temporal frequencies and spatial resolutions. Plankton samples were collected at 6 depths (3, 6, 9, 12, 18, 24 m) using a pump, every 2-h over a 36- and a 25-h period, during a spring and neap tide, respectively, concurrently with measures of temperature, salinity, fluorescence and current velocity. For 10 gastropod taxa, larval vertical distribution was mostly related to the thermal structure of the water column. Each of 7 taxonomic groups was found either exclusively near the surface, associated with the fluorescence maximum, or showed diel changes in distribution. These larvae that occupy different depths in the water column exhibit different dispersal potentials. / Biogeographical data contained in this thesis will be submitted to the Oceanographic Biogeographic Information System (OBIS) and may be accessed on-line at http://www.iobis.org

Macroalgal beds provide the ecological foundations for most shallow reef ecosystems in temperate environments. With distinctive canopies primarily of brown laminarian algae (northern hemisphere), or laminarian or fucalean algae (southern hemisphere), in many areas these habitats are at risk from human activity. Overexploitation, pollution, and other effects of coastal activities have resulted in significant habitat loss in coastal ecosystems, and human-induced climate change is now seen as a major threat to ecosystem health in marine systems. Understanding the impact of climate change is particularly important for habitat-forming ecosystem engineers like kelps, as these species form the basis of hierarchically organised communities and play a fundamental role in determining community structure and ecological processes. South eastern Australia has experienced increases in marine temperatures at nearly four times the global average, and there is now evidence that, in some locations, macroalgae communities are retreating in a manner consistent with ocean warming. Successful management of marine systems requires understanding ecosystem processes, particularly the patterns and magnitude of production. Macroalgal communities often show relatively low resistance to disturbance, yet rapid recovery once disturbances are removed, hence they are generally highly dynamic in response to environmental perturbations. As a result, macroalgae are likely to play an increasingly important role in buffering the short term/dynamic effects of climate change on temperate reef communities.Knowledge of the productivity of seaweed-dominated temperate reef systems is largely a synthesis from studies conducted over small spatial scales utilising a variety of methods that generally measure different characteristics of both individual seaweeds and collectively. As a result of the diversity of measurement methods, estimates of gross primary productivity (GPP), production potential, and macroalgal biomass for temperate reefs are numerous and variable. This can lead to challenges for ecologists attempting to amalgamate research findings to facilitate long-term, broad-scale perspectives or compare short-term research between spatially separated communities. However, to date there has been relatively little research to compare measurement approaches and quantify differences in productivity estimates across the different techniques.The present research provides a unique investigation into some of the techniques and methodology involved in measuring primary productivity in marine systems, particularly kelp forests, using the macroalgae Ecklonia radiata, Phyllospora comosa and Macrocystis pyrifera as study species. The work is based on both field and laboratory exploration of productivity measurements and associated parameters. In situ measurements of primary productivity (diel oxygen modelling, benthic oxygen exchange chambers) or PSII electron transport (PAM fluorometry) are compared, and the possibility of using acoustics as a means of quantifying oxygen production at large scales is explored, as has already been applied in seagrass beds. This thesis also provides an in depth investigation of the effect of variability in sampling methodology with regards to interpretation of PAM fluorometry-derived parameters. Chapter 2 investigates the acoustic properties of Ecklonia radiata. The density, sound speed and resulting adabiatic compressibility of E. radiata tissue were investigated in the laboratory. Four methods were developed and trialled to determine the intrinsic sound speed of Ecklonia radiata tissue based on measurement of the time of flight of an ultrasonic pulse, while compressibility was calculated from density measurements. The results show that Ecklonia radiata sound speed and density are higher, and compressibility lower, than that of seawater. Properties varied according to size and tissue type and the variation likely reflected differences in cell type, packing and structure as well as the concentrations of alginates and other carbohydrates. These are important considerations for acoustic propagation and the results provide valuable inputs for future acoustic work. Chapter 3 focuses on the acoustic modelling of different scenarios of primary production in a shallow water rocky reef environment of Fortescue Bay (Canoe Bay), Tasmania, where E. radiata dominates the canopy. In February 2012, the environment was continuously probed by acoustic signal transmission and monitored by a comprehensive set of oceanographic sensors with the aim to assess the potential for acoustics to quantify excess oxygen production in bubble form. Ray-theory acoustic modelling results indicate that ecologically-significant void fractions of oxygen in the canopy layer from production would be clearly seen in diel variation of propagation features such as the energy decay rate of the medium impulse response. The model can then be used to invert empirical data for retrieving void fraction. However, comparative analysis of part of FORTES 12 data and model suggests that no large excess of bubbles was produced by photosynthesis under the present environmental conditions, in contrast to earlier observations made in seagrasses. As a result, the use of acoustics as a means of measuring primary productivity in kelp could not be further explored during the course of this research.Chapter 4 provides a unique comparison of the estimates of photosynthetic O2 production rates in an Ecklonia radiata dominated community using three different measurement methods: diel oxygen GPP models, benthic oxygen exchange chambers, and electron transport rate from PAM fluorometry which is usually interpreted as a measure of production potential. All three methods were run concurrently in situ in Fortescue Bay, Tasmania. The first diel oxygen model was fitted to in situ measures of dissolved oxygen (DO) in the environment and demonstrated a good fit, however, a consequence of this approach is that large variation in oxygen production was predicted at low PAR levels. A second model was created which utilised an explicit relationship between DO production and PAR, but it didn’t represent DO at the surface as well as the first model. Importantly, the two models indicate similar daily production rates of the seaweed bed (all species combined) that are ~ 2 times that predicted for the kelp alone based on incubations in the benthic chambers and scaling for the average size of adult kelp sporophytes and their population density. Oxygen evolution from incubation of sporophytes in benthic chambers and PAM fluorometry derived electron transport rates showed similar patterns, but the results indicate that the latter method may overestimate potential photosynthesis. The results suggest that diel oxygen modelling, benthic oxygen exchange chambers and PAM fluorescence can all provide good indications of productivity in shallow water marine environments. However, care must be taken in interpretation of results as each method differs in the type of productivity estimates it provides. As a direct measure of total seaweed production per unit area of reef, estimates from models based on empirical measures of environmental DO have much to recommend them.Chapter 5 details a final analysis investigating the effects of diurnal, seasonal and latitudinal variability in ambient light on PAM-derived parameters, as well as possible effects associated with depth, within- and between-alga variation in PSII performance, and latitudinal effects unrelated to the light climate. This research was based on field measurements undertaken in Tasmania, Western Australia and New South Wales, Australia in both summer and winter during 2012 and 2013, focussing on Ecklonia radiata, Macrocystis pyrifera and Phyllospora comosa. Photosynthetic characteristics of all species were highly dependent on the time of day, depth, latitude/region, season, and part of the thallus from which measurements were taken. Patterns dependent on time-of-day, depth and thalli placement varied with season and/or geographic region, and the nature of these patterns varied between species. It is clear from this work that efforts to standardise approaches to taking measurements of seaweeds using PAM fluorometry will be essential if measurements are to be compared meaningfully across studies.The key findings of this thesis are: (1) a first determination of the acoustic properties of E. radiata tissue which enable the development of scattering models to interpret scientific echosounder data collected in kelp beds; (2) a Gaussian beam/finite element beam code (Bellhop) with detailed environmental input and a huge number of beams can predict the acoustic character of a shallow water rocky reef and bubble layers with low-frequency effective sound speed; (3) the model allows prediction of the acoustic energy decay rates due to various scenarios of ecologically-relevant photosynthetic O2 production rates; (4) day vs night acoustic measurement and model data comparisons challenge void fraction predictions made from well established measurements and methods; (5) diel oxygen modelling, benthic oxygen exchange chambers and PAM fluorescence can all provide good indications of productivity, however, understanding the limitations of each method is essential when interpreting the results as the measurements they provide are not directly comparable; and (6) applying a consistent sampling methodology is a key consideration when planning research utilising PAM fluorometry as diurnal, seasonal, and latitudinal variability, as well as effects associated with depth and within- and between-alga variation in PSII performance will have significant impact on PAM-derived parameters. The results of this work give valuable insight into the advantages and disadvantages involved with several main techniques currently utilised to measure production of macroalgal/seagrass beds, and the challenges faced by ecologists attempting to interpret results and compare research between methods and across studies. Last but not least, this study provides important and relevant information on the potential use of acoustics as a future means of determining productivity of benthic habitat on large scales in marine environments. The work presented herein will assist in both development and interpretation of future study of productivity in marine systems. / Doctorat en Sciences de l'ingénieur et technologie / info:eu-repo/semantics/nonPublished

Made available in DSpace on 2014-12-17T14:55:13Z (GMT). No. of bitstreams: 1 JoemiaLGMM_TESE.pdf: 3140610 bytes, checksum: 8bdf88d29c888d95e74fd24e403e60fc (MD5) Previous issue date: 2013-06-07 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Ceramic substrates have been investigated by researchers around the world and has achieved a high interest in the scientific community, because they had high dielectric constants and excellent performance in the structures employed. Such ceramics result in miniaturized structures with dimensions well reduced and high radiation efficiency. In this work, we have used a new ceramic material called lead zinc titanate in the form of Zn0,8Pb0,2TiO3, capable of being used as a dielectric substrate in the construction of various structures of antennas. The method used in constructing the ceramic combustion synthesis was Self- Sustained High Temperature (SHS - "Self-Propagating High-Temperature Synthesis") which is defined as a process that uses highly exothermic reactions to produce various materials. Once initiated the reaction area in the reaction mixture, the heat generated is sufficient to become self-sustaining combustion in the form of a wave that propagates converting the reaction mixture into the product of interest. Were analyzed aspects of the formation of the composite Zn0,8Pb0,2TiO3 by SHS powders and characterized. The analysis consisted of determining the parameters of the reaction for the formation of the composite, as the ignition temperature and reaction mechanisms. The production of composite Zn0,8Pb0,2TiO3 by SHS performed in the laboratory, was the result of a total control of combustion temperature and after obtaining the powder began the development of ceramics. The product was obtained in the form of regular, alternating layers of porous ceramics and was obtained by uniaxial pressing. 10 The product was characterized by analysis of dilatometry, X-ray diffraction analysis and scanning electron microscopy. One of the contributions typically defined in this work is the development of a new dielectric material, nevertheless presented previously in the literature. Therefore, the structures of the antennas presented in this work consisted of new dielectric ceramics based Zn0,8Pb0,2TiO3 usually used as dielectric substrate. The materials produced were characterized in the microwave range. These are dielectrics with high relative permittivity and low loss tangent. The Ansoft HFSS, commercial program employee, using the finite element method, and was used for analysis of antennas studied in this work / Substratos cer?micos t?m sido investigados por pesquisadores de todo o mundo e tem despertado um grande interesse na comunidade cient?fica, pelo fato de apresentarem altas constantes diel?tricas e um excelente desempenho nas estruturas empregadas. Tais cer?micas resultam em estruturas miniaturizadas, com dimens?es bem reduzidas e de alta efici?ncia de radia??o. Neste trabalho, utilizou-se um novo material cer?mico, chamado de titanato de zinco chumbo, na forma de Zn0,8Pb0,2TiO3, capaz de ser utilizado como substrato diel?trico na constru??o de diversas estruturas de antenas. O m?todo utilizado na constru??o da cer?mica foi a S?ntese por Combust?o Auto-Sustentada a Alta Temperatura (SHS Self-Propagating High-Temperature Synthesis ) que ? definido como um processo que utiliza rea??es altamente exot?rmicas para produzir os mais diversos materiais. Uma vez iniciada a rea??o numa ?rea da mistura reagente, o calor gerado ? suficiente para tornar a combust?o auto-sustent?vel, na forma de uma onda que se propaga convertendo a mistura reagente no produto de interesse. Foram analisados os aspectos da forma??o do comp?sito Zn0,8Pb0,2TiO3 por SHS e os p?s obtidos, foram caracterizados. A an?lise consistiu na determina??o dos par?metros da rea??o para a forma??o do comp?sito, como a temperatura de igni??o e os mecanismos de rea??o. A produ??o do comp?sito Zn0,8Pb0,2TiO3 por SHS, realizada em laborat?rio, foi resultado de um controle total da temperatura de combust?o e ap?s a obten??o do p? 8 iniciou-se o desenvolvimento das cer?micas. O produto foi obtido na forma de camadas regulares e altern?ncia de porosidades e as cer?micas foram obtidas por prensagem uniaxial. O produto foi caracterizado atrav?s de an?lises de dilatometria, difra??o de raios-X e an?lise de microscopia de varredura eletr?nica. Uma das contribui??es principais deste trabalho foi o desenvolvimento de um novo material diel?trico, n?o obstante apresentado anteriormente na literatura. Portanto, as estruturas das antenas apresentadas neste trabalho foram constitu?das por novas cer?micas diel?tricas ? base de Zn0,8Pb0,2TiO3, geralmente utilizado como substrato diel?trico. Os materiais desenvolvidos foram caracterizados na faixa de micro-ondas. S?o substratos diel?tricos de alta permissividade relativa e baixa tangente de perda. O software comercial empregado, Ansoft HFSS, baseado no m?todo dos elementos finitos, foi utilizado na an?lise das antenas estudadas neste trabalho

Made available in DSpace on 2014-12-17T15:01:54Z (GMT). No. of bitstreams: 1 PriscilaVDMQ_TESE.pdf: 3706825 bytes, checksum: a3b44c4a57772c9356ce81d22bdb9174 (MD5) Previous issue date: 2012-03-19 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The green bean has organoleptic and nutritional characteristics that make it an important food source in tropical regions such as the Northeast of Brazil. It is a cheap source of protein and important for nutrition of rural population contributing significantly in subsistence farming of the families from Brazil s northeast. It is consumed in entire region and together with the dry meat and other products composes the menu of typical restaurants, being characterized as an important product for economy of Northeast. The green bean is consumed freshly harvested and has short cycle, being characterized as a very perishable food, which hampers your market. The drying method is an alternative to increase the lifetime and provide a reduction volume of this product making easier your transportation and storage. However is necessary to search ways of drying which keep the product quality not only from the nutritional standpoint but also organoleptic. Some characteristics may change with the drying process such as the coloring, the rehydration capacity and the grains cooking time. The decrease of drying time or of exposure of the grains to high temperature minimizes the effects related with the product quality loss. Among the techniques used to reduce the drying time and improve some characteristics of the product, stands out the osmotic dehydration, widely used in combined processes such as the pretreatment in drying food. Currently the use of the microwaves has been considered an alternative for drying food. The microwave energy generates heat inside of materials processed and the heating is practically instantaneous, resulting in shorter processing times and product quality higher to that obtained by conventional methods. Considering the importance of the green beans for the Northeast region, the wastefulness of production due to seasonality of the crop and your high perishability, the proposal of this thesis is the study of drying grain by microwaves with and without osmotic pretreatment, focusing on the search of conditions of processes which favor the rehydration of the product preserving your organoleptic characteristics. Based on the analysis of the results of osmotic dehydration and dielectric properties was defined the operating condition to be used in pretreatment of the green bean, with osmotic concentration in saline solution containing 12,5% of sodium chloride, at 40?C for 20 minutes. The drying of green bean by microwave was performed with and without osmotic pretreatment on the optimized condition. The osmotic predehydration favored the additional drying, reducing the process time. The rehydration of dehydrated green bean with and without osmotic pretreatment was accomplished in different temperature conditions and immersion time according to a factorial design 22, with 3 repetitions at the central point. According to results the better condition was obtained with the osmotically pretreated bean and rehydrated at a temperature of 60?C for 90 minutes. Sensory analysis was performed comparing the sample of the green bean in natura and rehydrated in optimized conditions, with and without osmotic pretreatment. All samples showed a good acceptance rate regarding the analyzed attributes (appearance, texture, color, odor and taste), with all values above 70%. Is possible conclude that the drying of green bean by microwave with osmotic pretreatment is feasible both in respect to technical aspects and rehydration rates and sensory quality of the product / O feij?o verde apresenta caracter?sticas organol?pticas e nutricionais que fazem dele uma importante fonte aliment?cia em regi?es de clima tropical como o Nordeste do Brasil. ? uma fonte de prote?na barata e importante para a nutri??o da popula??o rural contribuindo significativamente na agricultura de subsist?ncia das fam?lias nordestinas. ? consumido em toda a regi?o e comp?e junto com a carne de sol e outros produtos o card?pio dos restaurantes t?picos, caracterizando-se assim como um produto importante para a economia da Nordeste. O feij?o verde ? consumido rec?m-colhido e apresenta ciclo curto, sendo caracterizado como um alimento de alta perecibilidade, o que dificulta a sua comercializa??o. A secagem desse produto se caracteriza como uma alternativa para o aumento da vida ?til deste alimento, al?m de proporcionar uma redu??o de volume facilitando seu manuseio no transporte e armazenagem. Todavia necess?rio se faz buscar formas de secagem que preservem a qualidade do produto n?o s? sobre o ponto de vista nutricional como organol?ptico. Dentre as caracter?sticas que se alteram com o processo de secagem, destacam-se a colora??o, a capacidade de reidrata??o e o tempo de cozimento dos gr?os. A diminui??o do tempo de secagem, ou de exposi??o dos gr?os a elevadas temperaturas minimizam os efeitos relacionados com a perda de qualidade do produto. .Dentre as t?cnicas empregadas para minimizar o tempo de secagem e melhorar algumas caracter?sticas do produto, destaca-se a desidrata??o osm?tica, muito utilizada em processos combinados como pr?-tratamento na secagem dos alimentos. Atualmente a utiliza??o do micro-ondas tem sido apontada como uma alternativa para a secagem de alimentos. A energia de micro-ondas gera calor no interior dos materiais processados e o aquecimento ? praticamente instant?neo, o que resulta em tempos de processamento mais curtos e qualidade do produto superior ? obtida pelos m?todos convencionais. Considerando a import?ncia do feij?o verde para a regi?o Nordeste, o desperd?cio da produ??o devido a sazonalidade da safra e sua alta perecibilidade, foi proposto nesta tese o estudo da secagem dos gr?os em micro-ondas com e sem pr?-tratamento osm?tico, tendo como foco principal a busca das condi??es de processo que favore?am a reidrata??o do produto, com preserva??o de suas caracter?sticas organol?pticas. A partir da an?lise dos resultados da desidrata??o osm?tica e propriedades diel?tricas foi definida a condi??o operacional a ser utilizada no pr?-tratamento do feij?o verde, com concentra??o osm?tica em solu??o salina contendo 12,5% de cloreto de s?dio, a 400C por 20 minutos. A secagem do feij?o verde por micro-ondas foi realizada sem e com pr?-tratamento osm?tico na condi??o otimizada. A pr?-desidrata??o osm?tica favoreceu a secagem complementar, reduzindo o tempo de processo. A reidrata??o do feij?o verde desidratado com e sem pr?-tratamento osm?tico foi realizada em diferentes condi??es de temperatura e tempo de imers?o de acordo com um planejamento fatorial 22, com 3 repeti??es no ponto central . De acordo com os resultados a melhor condi??o, foi obtida com o feij?o pr?-tratado osmoticamente e reidratado a uma temperatura de 600C por 90 minutos. Realizou-se a an?lise sensorial comparando-se as amostra de feij?o verde in natura e reidratado nas condi??es otimizadas, com e sem pr?-tratamento osm?tico. Todas as amostras apresentaram um bom ?ndice de aceita??o referente aos atributos analisados (apar?ncia, textura, cor, odor e sabor), estando todos os valores acima de 70%. Conclui-se que a secagem do feij?o verde por micro-ondas com pr?-tratamento osm?tico ? vi?vel, tanto em rela??o aos aspectos t?cnicos como aos ?ndices de reidrata??o e a qualidade sensorial do produto

Les forçages physiques conditionnent la discontinuité dans l'espace et le temps (patchiness) du plancton dans l'océan. La thèse s'est basée sur deux exemples. Le premier concerne le nord-ouest des Sargasses où une série temporelle à du macrozooplankton a été analysée à partir du rétro signal acoustique (Sv) mesuré avec un ADCP 153-KHz. Trois types de tourbillons ont été identifiés: un tourbillon cyclonique productif, la périphérie d'un tourbillon "mode-water", et la périphérie d'un tourbillon anticyclonique. Les valeurs de Sv ont augmenté au cours du passage des tourbillons, avec une hausse plus marquée associée au bord des tourbillons cyclonique et anticyclonique, ce qui suggère une réponse biologique significative aux upwelling localisées dans la zone frontale de ces tourbillons. Dans le deuxième exemple, la distribution spatiale et temporelle du zooplancton a été étudiée au large de la Nouvelle-Calédonie au cours de deux campagnes multidisciplinaires en 2011. La variabilité du zooplancton a été évaluée à l'aide d'échantillonnage au filet ainsi qu'à partir de mesures acoustiques (ADCP embarqué, échosondeur scientifique et TAPS). Des amplitudes plus élevées de la migration verticale nycthémérale (DVM) du zooplancton étaient associées à une plus grande abondance de petit zooplancton et aux eaux froides du sud de la zone d'étude, tandis que des amplitudes de DVM plus faibles dans le nord étaient associés à des eaux plus chaudes et à de plus grande abondance des grands organismes. Ces mesures acoustique ont clairement mis en évidence le rôle des forçage physique, notamment des structures à méso-échelle, sur la répartition spatiale et temporelle du zooplancton. / Physical forcing drives the space and time discontinuity (patchiness) of plankton in the ocean. The thesis was focused on the role of these forcing on the zooplankton, studied using both acoustic and traditional methods with net sampling. The study was based on two examples. The first one concerns the northwestern Sargasso Sea where high resolution time-series data on 0-200m macrozooplankton abundance and distribution off Bermuda was estimated from volume backscattering strength (Sv) measured with a 153-Khz ADCP. Three types of eddies were identified: a productive cyclonic eddy, the periphery of a mode water eddy, and the periphery of an anticyclonic eddy. Sv values increased during passage of theses eddies, with a more pronounced increase associated with the edge of the cyclonic and the anticyclonic eddies, suggesting a significant biological response to localized upwelling in the high velocity boundary of these eddies. In the second example, spatial and temporal distribution of zooplankton off New Caledonia was studied during two multidisciplinary cruises in 2011. Zooplankton variability was assessed using net sampling together with acoustic measurements (shipborne ADCP, scientific echosounder and TAPS). Higher amplitudes of diel vertical migration (DVM) of zooplankton were associated with higher abundance of large zooplankton and cold waters to the south of the study area, while lower DVM amplitudes in the north were associated with warmer waters and higher abundance of small organisms. These acoustic measurements clearly evidenced the role of physical forcing, particularly mesoscale features, in shaping zooplankton space and time distribution.

Fish encounter daily cycles of hypoxia in the wild, but the physiological strategies for coping with repeated cycles of normoxia and hypoxia (intermittent hypoxia) are poorly understood. Contrastingly, the physiological strategies for coping with continuous (constant) exposure to hypoxia have been studied extensively in fish. The main objective of this thesis was to understand how Fundulus killifish cope with a diurnal cycle of intermittent hypoxia, an ecologically relevant pattern of aquatic hypoxia in the natural environment. To do this, I characterized the effects of intermittent hypoxia on hypoxia tolerance, oxygen transport, metabolism, and the oxidative stress defense system of killifish, and compared these effects to fish exposed to normoxia, a single cycle of hypoxia-normoxia, and constant hypoxia. Specifically, I studied the following topics: (i) how acclimation to intermittent hypoxia modifies hypoxia tolerance, and the hypoxia acclimation response of Fundulus heteroclitus (Chapter 2), (ii) metabolic adjustments occurring during a hypoxia-reoxygenation cycle (Chapter 3), (iii) how acclimation to intermittent hypoxia alters O2 transport capacity and maximal aerobic metabolic rate (Chapter 4), (iv) the effects of hypoxia and reoxygenation on reactive oxygen species and oxidative stress (Chapter 5), and (v) variation in hypoxia tolerance and in the hypoxia acclimation responses across Fundulus fishes (Chapter 6). Killifish rely on a unique and effective physiological strategy to cope with intermittent hypoxia, and that this strategy is distinct from both the response to a single bout of acute hypoxia-reoxygenation (12 h hypoxia followed by 6 h reoxygenation) and to chronic exposure to constant hypoxia (24 h hypoxia per day for 28 d). Key features of the acclimation response to intermittent hypoxia include (i) maintenance of resting O2 consumption rate in hypoxia followed by a substantial increase in O2 consumption rate during recovery in normoxia, (ii) reversible increases in blood O2 carrying capacity during hypoxia bouts, (iii) minimal recruitment of anaerobic metabolism during hypoxia bouts, and (iv) protection of tissues from oxidative damage despite alterations in the homeostasis of reactive oxygen species and cellular redox status. Of these features, (i) is unique to intermittent hypoxia, (ii) also occurs in fish exposed to acute hypoxia-reoxygenation, and (iii) and (iv) are observed in both fish acclimated to intermittent hypoxia as well as those acclimated to constant hypoxia. This is the most extensive investigation to date on how fish cope with the energetic and oxidative stress challenges of intermittent hypoxia, and how these responses differ from constant hypoxia. This thesis adds substantial insight into the general mechanisms by which animals can respond to an ecologically important but poorly understood feature of the aquatic environment. / Dissertation / Doctor of Philosophy (PhD) / Oxygen levels in the aquatic environment are dynamic. Many fishes routinely encounter changes in oxygen content in their environment. However, we have very little understanding of how cycles between periods of low oxygen (hypoxia) and periods of high oxygen (normoxia) affect the physiology of fish. This thesis investigated how Fundulus killifish cope with daily cycles between hypoxia and normoxia (intermittent hypoxia) by modifying oxygen transport, metabolism, and oxidative stress defense systems. I found that killifish rely on a unique and effective physiological strategy to cope with intermittent hypoxia, and that this strategy is distinct from how they respond to a single bout of hypoxia (followed by normoxia) and to a constant pattern of only hypoxia. This is the most extensive investigation to date on how fish respond to the challenges of intermittent hypoxia, an understudied but ecologically important type of aquatic hypoxia.