The role of cholesterol-rich diet and of high protein supplement on the development of a glomerular lesion was studied in male guinea pigs. The possible pathogenesis of lipid-induced glomerulosclerosis was investigated. Four experiments were carried out. Four groups of guinea pigs were used in experiment I: CONT group was kept on normal guinea pig chow for 70 days; HC group was kept on 2% cholesterol diet for 70 days; HP group was kept on 50% casein diet for 70 days, and HCHP group received 2% cholesterol diet for 30 days and 2% cholesterol/50% casein diet for another 40 days. In experiment II two groups were used: CONT group and acetyl phenylhydrazine (APH)-treated group in which haemolytic anaemia was induced. In the third experiment the same dietary regimens as described in experiment I were used. In experiment IV three groups, namely CONT, HC, and HCHP, were employed. The animals in experiment IV were sacrificed after 5, 10, and 30 days. The first experiment explored the role of high cholesterol - and high cholesterol/high protein diet in the development of glomerulosclerosis. The other three experiments were designed to learn about the possible mechanism of lipid-induced glomerulosclerosis. Lipid analyses of plasma, erythrocytes and kidney tissue as well as complete blood count, erythrocyte osmotic fragility and blood cell morphology studies were performed. Kidney histology, histochemistry, immunohistochemistry, electron microscopy, morphometry, and renal and liver function tests were also carried out. De novo cholesterol synthesis was assessed by measuring HMG COA reductase activity and incorporation of tritiated water into cholesterol in the kidneys. Cholesterol-fed animals showed decreased weight gain, increased cholesterol concentration in plasma, erythrocytes, and kidney tissue. Haemolytic anaemia was documented after 70 days on this dietary regimen. Glomerular proliferation lesion was first noted at day 30 and progressed by day 70. Moderate proteinuria and haematuria were observed at day 70. Addition of protein to the high cholesterol diet led to a further decrease in weight gain. It also increased the mortality rate to 40% by day 70. The glomerular lesion, proteinuria and haematuria, and possibly haemolysis were more marked in the HCHP group. No causal relationship was found between liver function, immune complexes, haemolysis and glomerulosclerosis. Serum phosphate levels did not differ among the groups. The lipid found in the kidney of both HC and HCHP groups was mostly of plasma origin, since the kidney cholesterol de novo synthesis was suppressed in these two groups compared to the CONT group. There was a concommitant increase in the lipid content of kidney tissue and the mesangial expansion (MA/GTA) at day 30. No significant increase in the intraglomerular monocyte/macrophage was found at day 30 in the HCHP group compared to the HC group. However, a significant correlation (r=0.678, p 0.001) was found between the number of these cells and MA/GTA ratio among the four experimental groups at day 70. These data indicate that lipid deposits in kidney tissue may induce a glomerulosclerotic lesion in the absence of monocytes. However, these cells likely augment the proliferation of mesangial cells. We postulate that high protein diet could worsen the lipid-induced glomerular lesion by increasing delivery of abnormal lipoproteins to the kidney which could trigger mesangial cellular proliferation directly and indirectly by a macrophage-mediated process. / Medicine, Faculty of / Pathology and Laboratory Medicine, Department of / Graduate

M.Tech. (Biological and Health Technology) / The manner in which ritual slaughterings are executed in the R.S.A. is unknown to the majority of the population. The requirements with which religious slaughterings must comply as well as the facilities used for that purpose are even less familiar. The subject of the Jewish method of slaughter (Shechita) has especially elicited much discussion, due to the major divergence of opinion between the Jewish community and animal welfare organisations, as far as the slaughter technique and restraining facilities are concerned. This study was undertaken in an endeavour to find a solution to the problems in the form of restraining facilities acceptable to both groups. The first phase comprised a literature study of Shechita. The second phase consisted of a study tour to the United States of America and Israel, to become acquainted with the latest technology employed for Shechita. The last phase entailed the construction of a prototype restrainer facility at the Johannesburg Abattoir to facilitate experiments for the development of a head clamp. The function of the head clamp is to restrain the animal in such a manner that it can be slaughtered in the upright position but still in full compliance with the rules of Shechita. This study represents an endeavour to contribute meaningfully to and supplement existing knowledge and know-how applicable in South African circumstances.

Arsenic (As) exposure is associated with cancer, lung and cardiovascular disease, yet the mechanisms involved are not clearly understood. Elevated matrix metalloproteinase-9 (MMP-9) levels are also associated with these diseases, as well as with exposure to water As. Our objective was to evaluate the effects of dietary components of inorganic As (iAs) intake on serum MMP-9 concentration at differing levels of tap water As. In a cross-sectional study of 214 adults, dietary iAs intake was estimated from 24-h dietary recall interviews using published iAs residue data; drinking and cooking water As intake from water samples and consumption data. Aggregate iAs intake (food plus water) was associated with elevated serum MMP-9 in mixed model regression, with and without adjustment for covariates. In models stratified by tap water As, aggregate intake was a significant positive predictor of serum MMP-9 in subjects exposed to water As≤10 μg/l. Inorganic As from food alone was associated with serum MMP-9 in subjects exposed to tap water As≤3 μg/l. Exposure to iAs from food and water combined, in areas where tap water As concentration is ≤10 μg/l, may contribute to As-induced changes in a biomarker associated with toxicity.

京都大学 / 0048 / 新制・論文博士 / 博士(医学) / 乙第13289号 / 論医博第2187号 / 新制||医||1039(附属図書館) / (主査)教授 中山 健夫, 教授 柳田 素子, 教授 佐藤 俊哉 / 学位規則第4条第2項該当 / Doctor of Medical Science / Kyoto University / DFAM

Background: Bowel preparation is essential for quality colonoscopy. Although most bowel preparation regimens recommend dietary restriction for 24 to 48 hours before the procedure, the evidence for this is poor. Objectives: To establish whether dietary restriction during bowel preparation improves the quality of bowel preparation. Methods: A prospective single blind, randomised controlled pilot study. The dietary restriction (DR) group was instructed not to ingest high fibre foods for 48 hours prior to the use of a polyethylene glycol (PEG) bowel preparation. The non-dietary restriction (NDR) group was not given any dietary modification, but received instructions for the use of the PEG-based preparation solution. On the day of colonoscopy, the quality of the bowel effluent was assessed, and additional preparation given as necessary. The primary endpoint was quality of bowel cleansing using the Harefield Cleansing Scale during colonoscopy. The secondary endpoint was the need for additional bowel preparation and quantity of additional bowel preparation given prior to endoscopy. Data were analysed on an intention to treat basis. Results: Twenty-three participants were randomised to the intervention group and thirty-four to the control group. Patient demographics were similar in both groups. Dietary restriction did not influence the success rate of bowel preparation: 97% successful bowel preparation in the DR group, vs 91% successful bowel preparation in the NDR group (p=0.559). Additional bowel preparation requirement were similar in both groups: 35% in DR group vs 39% in NDR group (p=0.768). Mean amount of additional bowel preparation required was similar: 560 ml in the DR group vs 460 ml in the NDR group (p=0.633). Conclusion: The quality of bowel preparation was comparable in patients with and without dietary restrictions prior to colonoscopy. Non-restrictive diets prior to bowel preparation should be considered to increase compliance. The sample size of this pilot study prohibited definite statistical conclusions but demonstrated this to be a reasonable methodology for a larger study.

This study was conducted to evaluate the kernel, flour and dough qualities and dietary fiber content of hulled wheats. Experimental design was separate randomized complete block designs for hulled wheat species with four field replicates. According to the results, significant differences (p<0.05) were observed in kernel quality, flour, and dough quality compared to common bread wheat. Einkorn and spelt reported significantly lower insoluble dietary fiber and total dietary fiber content, in contrast emmer had contents with both higher and lower genotypes. Interestingly, few genotypes of hulled wheat had a higher content of low molecular weight soluble dietary fibers (LMW-SDF) such as fructo and galacto oligosaccharides. Overall, hulled wheats differed from modern bread wheat in their kernel, flour, baking and nutritional quality. Moreover, due to higher LMW-SDF content, hulled wheats would be a potential candidate for breeding and producing health beneficial novel food products.

This study was undertaken to determine the effect of protein and wheat germ oil supplements upon selected measures of junior high school boys. The measures selected were for weight, arm strength, speed and endurance, and urinalyses for urea nitrogen. Thirty-six ninth grade athletes at Memorial Junior High School in Alice, Texas, were used as subjects. The subjects were placed in three groups of twelve each with one group receiving the supplements and the other two groups serving as control groups.

No description available.

The loss of muscle (mass, strength, and function) occurs in many individuals in their 40’s and begins to accelerate after 50 years of age resulting from multiple understood mechanisms. While many factors are thought to contribute to the changes that are observed in muscle with aging, one significant, yet modifiable factor is dietary protein intake. This study proposed to improve dietary protein intake in a group of middle-aged women living in South Florida over a 12-week period using individualized per-meal protein prescriptions (1.2 g protein/kg body weight evenly distributed for all three meals) provided by a Registered Dietitian, followed by weekly diet coaching from a Nurse Practitioner (coach). Aims included: (1) An examination of the effect of diet coaching on protein intake, nutrition knowledge, muscle mass, strength, and function and (2) An understanding of how diet coaching sessions help in the process of making dietary changes. A parallel mixed methods research design allowed for a synthesis of inferences from each aim resulting in a meta-inference highlighting interdisciplinary collaboration. / Includes bibliography. / Dissertation (Ph.D.)--Florida Atlantic University, 2020. / FAU Electronic Theses and Dissertations Collection

Methods currently used to determine iron bioavailability have disadvantages for both the subjects and researchers involved. One safe and simple method that needs further evaluation, the serum iron absorption method, involves an initial blood drawing, dosage s of iron, and blood drawings taken at intervals thereafter. Generally, the rise in serum iron or area under the curve is used to determine iron uptake. Two experiments were conducted using the serum iron absorption method in an effort to improve the utility of this method for the measurement of iron bioavailability. With this effort in mind, an equation adjusting for dose of iron (0.5-8 mg) and blood volume was used to estimate serum iron absorption and allow for adequate comparisons of other iron absorption data obtained with this method and others. One very significant result was that low serum ferritin was found to be a predictor of high serum estimated iron absorption (SEIA). Similarly, low serum ferritin levels were also highly correlated to iron absorption when radioiron methods were used. In Experiment I, college-aged women (n=11) with low serum ferritin levels (average 11 ng/ml) participated in four serum iron trials in which four iron sources were used as iron dosages. Ferrous sulfate in orange juice (2.2 mg iron), ground beef (0.62 mg iron), cereal (6.6 mg iron), and ground beef and cereal together (3.6 mg iron) were used. Comparisons were made between the results obtained using this serum iron absorption data and other radioiron absorption data for similar iron sources, and the results were quite similar to iron absorption levels reported in the literature. In Experiment I, the ferrous sulfate (40%) and ground beef treatments (46%) had the highest serum estimated iron absorption (SEIA) and the cereal/ground beef (5%) and cereal treatments (3%) had significantly less. Experiment II involved 28 college-aged women who participated in four serum iron absorption trials. For these four trials the iron dose was the same (2 mg iron as ferrous sulfate in orange juice). However, pre-trial supplements containing either (1) 30 mg iron, 60 mg ascorbic acid, (2) 30 mg iron, no ascorbic acid, (3) no iron, 60 mg ascorbic acid, or (4) a placebo were given (one per day) on each of the three days prior to the weekly serum iron absorption trials. During each pre-trial supplementation period, three daily diet records were kept by subjects to determine if the previous intake of nutrients (from food or pre-trial supplements) influenced the outcome of the serum iron absorption trial. Pre-trial supplementation of 30 mg iron, no ascorbic acid caused a significantly lower SEIA. Dietary nutrients did not cause a significant effect on SEIA. Mean SEIA following combined iron supplements was lower (p=0.081) 16% vs. 23% following non-iron containing supplements. For this experiment, the SEIA was higher in subjects with low serum ferritin (20 ng/ml).