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Stability and clinical efficacy of honeybush extracts in cosmeceutical productGerber, Gezina Susanna Fredrika Wilhelmina January 2012 (has links)
The progression of skin ageing in individuals is multifaceted and provoked by various aspects, including hereditary and a variety of environmental causes, for instance UV (ultra violet) radiation, resulting in the morphological modifications in the dermal layer of the skin (Makrantonaki & Zouboulis, 2007:40) Transformations caused by ageing skin, in which degenerative alterations exceed regenerative alterations are recognised by the thinning and wrinkling of the epidermis in conjunction with the appearance of lines, creases, crevices and furrows, particularly emphasised in lines of facial expressions (Aburjai & Natsheh, 2003:990).
For human beings to continue to exist in a terrestrial atmosphere, the loss of water from the skin must be cautiously synchronised by the epidermis, a task dependent on the multifaceted character of the stratum corneum (Rawlings & Harding, 2004:43). The stratum corneum (SC) is responsible for the main resistance to the penetration of most compounds; nevertheless the skin represents as an appropriate target for delivery. The target site for anti-ageing treatment includes the epidermal and dermal layers of the skin. Therefore, the need to apply fatty materials to the skin is practically intuitive and may perhaps be as old as man’s existence itself (Lodén, 2005:672). Natural therapies have been used for several decades for taking care of skin illnesses and a wide variety of dermatological disorders, such as inflammation, phototoxicity, atopic dermatitis and alopecia areata (Aburjai & Natsheh, 2003:988).
Using the skin as an alternative route for the administration of honeybush extracts for the treatment of ageing skin may be beneficial. Tea contains more than 500 chemical compounds, including, tannins, flavonoids, amino acids, vitamins, caffeine and polysaccharides. Tea polyphenols (flavonoids) have proven anti-inflammatory, antioxidant, antiallergic, antibacterial and antiviral effects (Aburjai & Natsheh, 2003:990). Unfortunately using the skin as an alternative route for administering drugs (transdermal drug delivery) has numerous limitations.
One of these limitations is the barrier function of the skin (Naik et al., 2000:319). Because of the skin’s outstanding ability to protect the body against unwanted substances from its surroundings, it is necessary to use methods to enhance drug penetration through the skin.
The aim of this study was to formulate two 2% semisolid formulations containing two different honeybush extracts as the active ingredient, and to determine which of the formulations deliver mangiferin and hesperidin best to the target site (dermis). Cosmetic formulations of a natural origin, is designed to protect the skin against exogenous or endogenous harmful agents, as well as to balance the dermal homeostatis lipids altered by dermatosis and ageing (Aburjai & Natsheh, 2003:988).
Stability tests over a three month period were also performed on the different formulations. To determine the stability of the different semi-solid formulations, the formulated products were stored at 25 °C/60% RH (relative humidity), 30 °C/60% RH and 40 °C/75% RH. HPLC analysis was used to determine the concentrations of the ingredients in all the formulated products.
Other stability tests included appearance, pH, viscosity, mass loss, zeta potential and particle size determination. Unfortunately a change in colour, viscosity, zeta potential, mass loss, particle size and concentration of the ingredients in both the formulations, indicated that the products were unstable from the first month of stability testing. A 2% Cyclopia maculata cream as well as a 2% Cyclopia genistoides cream was formulated.
Franz cell diffusion studies as well as membrane release studies were performed over a 12 h period, followed by tape stripping experiments to determine which semi-solid formulation delivered mangiferin and hesperidin the best to the dermal layer of the skin. The results of the different formulations were compared. Unfortunately there was no significant penetration by any of the honeybush extracts. Results were inconclusive and unquantifiable due to unconvincing penetration results.
The antioxidant properties of both the extracts and the active ingredients were calculated. Antioxidant studies by the use of the TBA-assay method were done to determine whether the honeybush extracts, mangiferin and hesperidin as well as their semisolid formulations had any antioxidant activities. Both the honeybush extracts and the semisolid formulations showed promising results. Mangiferin and hesperidin did not show any antioxidant activity on their own, therefore the assumption can be confirmed that plants do function synergistically.
A clinical study was also conducted to see whether honeybush extracts have the potential to hydrate the skin, counteracting the symptoms and signs of skin ageing. Clinical efficacy studies were done to determine whether the honeybush formulations had any skin hydrating effects in the treatment against skin ageing. The results were statistically inconclusive and variations between the subjects were very high due to skin variations at different skin sites. There was however a trend that Cyclopia genistoides performed the best. / Thesis (MSc (Pharmaceutics))--North-West University, Potchefstroom Campus, 2013.
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Comparison of rat and porcine jejunum as in vitro models for P–glycoprotein mediated efflux using the Sweetana–Grass diffusion method / H.J. OosthuizenOosthuizen, Hendrik Jacobus January 2010 (has links)
Absorption of drug substances across the intestinal epithelium is a complex and dynamic process. Counter transport proteins are responsible for the efflux of specific drug molecules after they have been absorbed. One of the key counter transport efflux proteins, which is of importance in this study, is P–glycoprotein. The efflux pump P–glycoprotein plays a major role in altering the pharmacokinetics of a wide variety of drugs limiting their absorption and therefore also bioavailability. Many flavonoids have been shown to interact with P–glycoprotein mediated efflux in vitro studies. Numerous in vitro methods have been used to study drug absorption across the intestinal membranes, but it is often not possible to use only one in vitro model to accurately predict permeability characteristics.
The purpose of this study was to determine the effect of four selected hydroxy– and methoxy– flavonoids on the in vitro transport of Rhodamine 123, a known P–gp substrate, across excised rat and pig intestinal tissue using the Sweetana–Grass diffusion apparatus. The results were further used to determine if the two different animal tissue models corresponded with regard to the flavonoids' effects on P–glycoprotein related efflux. Two control groups were included in the experimental design. In the negative control group, the transport of Rhodamine 123 was tested alone and no modulator was added. In the positive control group, the transport of Rhodamine 123 was determined in the presence of Verapamil, which is a known P–glycoprotein inhibitor. The experiments with the flavonoids Morin, Galangin, 6–Methoxyflavone and 7–Methoxyflavone were done in triplicate to determine repeatability of the results. The transport of Rhodamine 123 was evaluated in both the apical to basolateral (absorptive) and basolateral to apical (secretory) directions. The relative transport of Rhodamine 123, the apparent permeability coefficient (P app) values and flux (J) values in both directions as well as the efflux ratio (ER) and net flux (J net) were calculated. The concentration Rhodamine 123 present in the acceptor chamber was determined by means of a validated HPLC method. Statistical analysis was used to compare the results of the test groups with the control groups in order to indicate significant differences.
It has been found that Morin, Galangin and 6–Methoxyflavone have a significant inhibitory effect on the Rhodamine 123 efflux (probably P–glycoprotein related) in both the rat and pig intestinal tissue models with p–values smaller than 0.05. On the other hand, 7–Methoxyflavone showed a significant effect on the efflux of Rhodamine 123 in the pig intestinal tissue model (p < 0.05) but not in the rat intestinal tissue model (p > 0.05). These flavonoids may increase the bioavailability of drugs that are substrates for P–glycoprotein and thereby cause clinically significant pharmacokinetic interactions, however, this should be confirmed with in vivo studies. On the other hand, these flavonoids may be used for drug absorption enhancement when applied under controlled circumstances.
With regard to the different animal tissue models used it can be concluded that data obtained from the rat intestinal tissue model cannot be compared and extrapolated to data obtained from the pig intestinal tissue model. It is recommended that the in vitro results be correlated to in vivo findings to identify the most suitable model. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2011.
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Cosmic ray modulation processes in the heliosphere / Vos E.E.Vos, Etienne Eben January 2011 (has links)
The solar minimum of 2009 has been identified as an exceptional event with regard to
cosmic ray (CR)modulation, since conditions in the heliosphere have reached unprecedented
quiet levels. This unique minimum has been observed by the Earth–orbiting
satellite, PAMELA, launched in June, 2006, from which vast sets of accurate proton
and electron preliminary observations have been made available. These simultaneous
measurements from PAMELA provide the ideal opportunity to conduct an in–depth
study of CR modulation, in particular charge–sign dependent modulation. In utilizing
this opportunity, a three–dimensional, steady–state modulation model was used to reproduce
a selection of consecutive PAMELA proton and electron spectra from 2006 to
2009. Thiswas done by assuming full drifts and simplified diffusion coefficients, where
the rigidity dependence and absolute value of themean free paths for protons and electrons
were sequentially adjusted below 3 GV and 300 MV, respectively. Care has
been taken in calculating yearly–averaged current–sheet tilt angle and magnetic field
values that correspond to the PAMELA spectra. Following this study where the numerical
model was used to investigate the individual effects resulting from changes in
the tilt angle, diffusion coefficients, and global drifts, it was found that all these modulation
processes played significant roles in contributing to the total increase in CR
intensities from 2006 to 2009, as was observed by PAMELA. Furthermore, the effect
that drifts has on oppositely charged particles was also evident from the difference
between the peak–shaped time profiles of protons and the flatter time profiles of electrons,
as is expected for an A < 0 polarity cycle. Since protons, which drift into the
heliosphere along the heliospheric current–sheet, haven’t yet reached maximum intensity
levels by 2008, their intensities increased notably more than electrons toward the
end of 2009. The time and energy dependence of the electron to proton ratios were
also studied in order to further illustrate and quantify the effect of drifts during this
remarkable solar minimum period. / Thesis (M.Sc. (Physics))--North-West University, Potchefstroom Campus, 2012.
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Comparison of rat and porcine jejunum as in vitro models for P–glycoprotein mediated efflux using the Sweetana–Grass diffusion method / H.J. OosthuizenOosthuizen, Hendrik Jacobus January 2010 (has links)
Absorption of drug substances across the intestinal epithelium is a complex and dynamic process. Counter transport proteins are responsible for the efflux of specific drug molecules after they have been absorbed. One of the key counter transport efflux proteins, which is of importance in this study, is P–glycoprotein. The efflux pump P–glycoprotein plays a major role in altering the pharmacokinetics of a wide variety of drugs limiting their absorption and therefore also bioavailability. Many flavonoids have been shown to interact with P–glycoprotein mediated efflux in vitro studies. Numerous in vitro methods have been used to study drug absorption across the intestinal membranes, but it is often not possible to use only one in vitro model to accurately predict permeability characteristics.
The purpose of this study was to determine the effect of four selected hydroxy– and methoxy– flavonoids on the in vitro transport of Rhodamine 123, a known P–gp substrate, across excised rat and pig intestinal tissue using the Sweetana–Grass diffusion apparatus. The results were further used to determine if the two different animal tissue models corresponded with regard to the flavonoids' effects on P–glycoprotein related efflux. Two control groups were included in the experimental design. In the negative control group, the transport of Rhodamine 123 was tested alone and no modulator was added. In the positive control group, the transport of Rhodamine 123 was determined in the presence of Verapamil, which is a known P–glycoprotein inhibitor. The experiments with the flavonoids Morin, Galangin, 6–Methoxyflavone and 7–Methoxyflavone were done in triplicate to determine repeatability of the results. The transport of Rhodamine 123 was evaluated in both the apical to basolateral (absorptive) and basolateral to apical (secretory) directions. The relative transport of Rhodamine 123, the apparent permeability coefficient (P app) values and flux (J) values in both directions as well as the efflux ratio (ER) and net flux (J net) were calculated. The concentration Rhodamine 123 present in the acceptor chamber was determined by means of a validated HPLC method. Statistical analysis was used to compare the results of the test groups with the control groups in order to indicate significant differences.
It has been found that Morin, Galangin and 6–Methoxyflavone have a significant inhibitory effect on the Rhodamine 123 efflux (probably P–glycoprotein related) in both the rat and pig intestinal tissue models with p–values smaller than 0.05. On the other hand, 7–Methoxyflavone showed a significant effect on the efflux of Rhodamine 123 in the pig intestinal tissue model (p < 0.05) but not in the rat intestinal tissue model (p > 0.05). These flavonoids may increase the bioavailability of drugs that are substrates for P–glycoprotein and thereby cause clinically significant pharmacokinetic interactions, however, this should be confirmed with in vivo studies. On the other hand, these flavonoids may be used for drug absorption enhancement when applied under controlled circumstances.
With regard to the different animal tissue models used it can be concluded that data obtained from the rat intestinal tissue model cannot be compared and extrapolated to data obtained from the pig intestinal tissue model. It is recommended that the in vitro results be correlated to in vivo findings to identify the most suitable model. / Thesis (M.Sc. (Pharmaceutics))--North-West University, Potchefstroom Campus, 2011.
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Cosmic ray modulation processes in the heliosphere / Vos E.E.Vos, Etienne Eben January 2011 (has links)
The solar minimum of 2009 has been identified as an exceptional event with regard to
cosmic ray (CR)modulation, since conditions in the heliosphere have reached unprecedented
quiet levels. This unique minimum has been observed by the Earth–orbiting
satellite, PAMELA, launched in June, 2006, from which vast sets of accurate proton
and electron preliminary observations have been made available. These simultaneous
measurements from PAMELA provide the ideal opportunity to conduct an in–depth
study of CR modulation, in particular charge–sign dependent modulation. In utilizing
this opportunity, a three–dimensional, steady–state modulation model was used to reproduce
a selection of consecutive PAMELA proton and electron spectra from 2006 to
2009. Thiswas done by assuming full drifts and simplified diffusion coefficients, where
the rigidity dependence and absolute value of themean free paths for protons and electrons
were sequentially adjusted below 3 GV and 300 MV, respectively. Care has
been taken in calculating yearly–averaged current–sheet tilt angle and magnetic field
values that correspond to the PAMELA spectra. Following this study where the numerical
model was used to investigate the individual effects resulting from changes in
the tilt angle, diffusion coefficients, and global drifts, it was found that all these modulation
processes played significant roles in contributing to the total increase in CR
intensities from 2006 to 2009, as was observed by PAMELA. Furthermore, the effect
that drifts has on oppositely charged particles was also evident from the difference
between the peak–shaped time profiles of protons and the flatter time profiles of electrons,
as is expected for an A < 0 polarity cycle. Since protons, which drift into the
heliosphere along the heliospheric current–sheet, haven’t yet reached maximum intensity
levels by 2008, their intensities increased notably more than electrons toward the
end of 2009. The time and energy dependence of the electron to proton ratios were
also studied in order to further illustrate and quantify the effect of drifts during this
remarkable solar minimum period. / Thesis (M.Sc. (Physics))--North-West University, Potchefstroom Campus, 2012.
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In vitro skin permeation of selected platinum group metals / Anja FrankenFranken, Anja January 2014 (has links)
Background: Platinum group metal (PGM) mining and refining is a large constituent of the mining sector of South Africa and contributes significantly to the gross domestic product. The PGMs include the rare metals platinum (Pt), palladium (Pd), rhodium (Rh), ruthenium (Ru), iridium (Ir) and osmium (Os). During the refining process workers are potentially exposed to various chemical forms of the PGMs via the respiratory and dermal exposure routes. Historically, emphasis has been on respiratory exposure while the extent of skin exposure is still unknown. Among the different forms of PGMs, the salts are potential sensitisers, with platinum being a known respiratory sensitiser. Workers occupationally exposed to platinum and rhodium have reported respiratory as well as skin symptoms. However, it is unknown if these metals in the salt form are permeable through human skin, and whether dermal exposure could contribute to sensitisation. Evidence regarding differences between African and Caucasian skin anatomy and structure, as well as permeation through skin is contradictory, and no information is available on metal permeation through African skin. The in vitro diffusion method has been utilised successfully in occupational toxicology to demonstrate that metals such as chromium, cobalt and nickel, to name a few, permeate through human skin. The permeability of platinum and rhodium has not been investigated previously.
Aims and objectives: The research aim was to obtain insight into the permeability of platinum and rhodium through intact human skin and to provide information needed to determine the potential health risk following dermal exposure to these metals. The specific objectives included: (i) to critically review the in vitro diffusion method that is used to determine the permeability of metals through human skin, (ii) to investigate the permeation of potassium tetrachloroplatinate (K2PtCl4) and rhodium chloride (RhCl3) as representative PGM salts through intact human skin over a 24-hour period, (iii) to evaluate the difference in permeability of platinum and rhodium through intact human skin, (iv) to evaluate the difference in permeability of platinum through intact African and Caucasian human skin.
Methods: Abdominal skin obtained after cosmetic procedures was obtained from five female Caucasian and three female African donors between the ages of 28 and 52 with ethical approval from the North-West University. Full thickness skin tissue was mounted in a vertical Franz diffusion cell. Skin integrity was tested by measuring the electrical resistance across the skin before and after conclusion of the experiments, using a Tinsley LCR Data bridge Model 6401. The donor solution of 32.46 mg K2PtCl4 in 50 ml of synthetic sweat (pH 6.5), and 43.15 mg RhCl3 in 50 ml of synthetic sweat (pH 6.5) was prepared. The donor solution was applied to the stratum corneum side of the skin and physiological receptor solution (pH 7.35) was added to the receptor compartment. The concentration of the metals in the receptor solution was determined by high resolution inductively coupled plasma-mass spectrometry after extraction at various intervals during the 24 hours of the study. After completion of the study, the skin was rinsed four times to remove any platinum or rhodium remaining on the skin surface. The skin was digested using hydrogen peroxide, nitric acid and hydrochloric acid during different steps to determine the mass of the metals remaining in the skin by inductively coupled plasma-optical emission spectrometry.
Results: The comparison of published in vitro skin permeation studies involving metals is impeded by the variations in the experimental design and dissimilarity in the reporting of results. Differences in experimental design included, most noticeably, the use of various donor and receptor solutions, different temperatures wherein the receptor compartment was placed, differences in skin thickness and variations in exposed skin surface areas. The metals considered in the review, namely chromium, cobalt, gold, lead, mercury, nickel, platinum, rhodium and silver, permeate through intact human skin under physiological conditions. Large variations in the permeability results were observed, with the notable differences in methodology as the probable reason. Results obtained from the in vitro experiments indicate that platinum and rhodium permeated through intact Caucasian skin with flux values of 0.12 and 0.05 ng/cm2/h, respectively. The cumulative mass of platinum (2.57 ng/cm2) that permeated after 24 hours of exposure was statistically significantly (p = 0.016) higher than rhodium permeation (1.11 ng/cm2). The mass of platinum (1 459.47 ng/cm2) retained in the skin after 24 hours of exposure was statistically significantly (p < 0.001) higher than rhodium retention (757.04 ng/cm2). The comparison of permeability between two different racial groups indicates that platinum permeated through the skin of both racial groups with the flux through African skin found as 1.93 ng/cm2/h and 0.27 ng/cm2/h through Caucasian skin. The cumulative mass of platinum permeated after 24 hours of exposure was statistically significantly (p = 0.044) higher through African skin (37.52 ng/cm2) than Caucasian skin (5.05 ng/cm2). The retention of platinum in African skin (3 064.13 ng/cm2) was more than twice the mass retained in Caucasian skin (1 486.32 ng/cm2).
Conclusions: The in vitro diffusion method is an applicable method to determine skin permeability of metals. However, the experimental design and format of data reporting should be standardised to enable comparison of results from different studies. Platinum and rhodium permeated through intact human skin, with platinum permeation significantly higher. African skin was significantly more permeable by platinum than Caucasian skin. Both platinum and rhodium were retained inside the skin after 24 hours of exposure, possibly forming a reservoir which could contribute to continued permeation through the skin even after removal thereof from the skin. Platinum and rhodium permeated through full thickness skin and thereby could possibly contribute to local skin symptoms such as dermatitis and urticaria found in occupationally exposed workers. By permeating through the upper layers of the skin, these metals could potentially reach the viable epidermis and contribute to sensitisation. / PhD (Occupational Hygiene), North-West University, Potchefstroom Campus, 2015
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In vitro skin permeation of selected platinum group metals / Anja FrankenFranken, Anja January 2014 (has links)
Background: Platinum group metal (PGM) mining and refining is a large constituent of the mining sector of South Africa and contributes significantly to the gross domestic product. The PGMs include the rare metals platinum (Pt), palladium (Pd), rhodium (Rh), ruthenium (Ru), iridium (Ir) and osmium (Os). During the refining process workers are potentially exposed to various chemical forms of the PGMs via the respiratory and dermal exposure routes. Historically, emphasis has been on respiratory exposure while the extent of skin exposure is still unknown. Among the different forms of PGMs, the salts are potential sensitisers, with platinum being a known respiratory sensitiser. Workers occupationally exposed to platinum and rhodium have reported respiratory as well as skin symptoms. However, it is unknown if these metals in the salt form are permeable through human skin, and whether dermal exposure could contribute to sensitisation. Evidence regarding differences between African and Caucasian skin anatomy and structure, as well as permeation through skin is contradictory, and no information is available on metal permeation through African skin. The in vitro diffusion method has been utilised successfully in occupational toxicology to demonstrate that metals such as chromium, cobalt and nickel, to name a few, permeate through human skin. The permeability of platinum and rhodium has not been investigated previously.
Aims and objectives: The research aim was to obtain insight into the permeability of platinum and rhodium through intact human skin and to provide information needed to determine the potential health risk following dermal exposure to these metals. The specific objectives included: (i) to critically review the in vitro diffusion method that is used to determine the permeability of metals through human skin, (ii) to investigate the permeation of potassium tetrachloroplatinate (K2PtCl4) and rhodium chloride (RhCl3) as representative PGM salts through intact human skin over a 24-hour period, (iii) to evaluate the difference in permeability of platinum and rhodium through intact human skin, (iv) to evaluate the difference in permeability of platinum through intact African and Caucasian human skin.
Methods: Abdominal skin obtained after cosmetic procedures was obtained from five female Caucasian and three female African donors between the ages of 28 and 52 with ethical approval from the North-West University. Full thickness skin tissue was mounted in a vertical Franz diffusion cell. Skin integrity was tested by measuring the electrical resistance across the skin before and after conclusion of the experiments, using a Tinsley LCR Data bridge Model 6401. The donor solution of 32.46 mg K2PtCl4 in 50 ml of synthetic sweat (pH 6.5), and 43.15 mg RhCl3 in 50 ml of synthetic sweat (pH 6.5) was prepared. The donor solution was applied to the stratum corneum side of the skin and physiological receptor solution (pH 7.35) was added to the receptor compartment. The concentration of the metals in the receptor solution was determined by high resolution inductively coupled plasma-mass spectrometry after extraction at various intervals during the 24 hours of the study. After completion of the study, the skin was rinsed four times to remove any platinum or rhodium remaining on the skin surface. The skin was digested using hydrogen peroxide, nitric acid and hydrochloric acid during different steps to determine the mass of the metals remaining in the skin by inductively coupled plasma-optical emission spectrometry.
Results: The comparison of published in vitro skin permeation studies involving metals is impeded by the variations in the experimental design and dissimilarity in the reporting of results. Differences in experimental design included, most noticeably, the use of various donor and receptor solutions, different temperatures wherein the receptor compartment was placed, differences in skin thickness and variations in exposed skin surface areas. The metals considered in the review, namely chromium, cobalt, gold, lead, mercury, nickel, platinum, rhodium and silver, permeate through intact human skin under physiological conditions. Large variations in the permeability results were observed, with the notable differences in methodology as the probable reason. Results obtained from the in vitro experiments indicate that platinum and rhodium permeated through intact Caucasian skin with flux values of 0.12 and 0.05 ng/cm2/h, respectively. The cumulative mass of platinum (2.57 ng/cm2) that permeated after 24 hours of exposure was statistically significantly (p = 0.016) higher than rhodium permeation (1.11 ng/cm2). The mass of platinum (1 459.47 ng/cm2) retained in the skin after 24 hours of exposure was statistically significantly (p < 0.001) higher than rhodium retention (757.04 ng/cm2). The comparison of permeability between two different racial groups indicates that platinum permeated through the skin of both racial groups with the flux through African skin found as 1.93 ng/cm2/h and 0.27 ng/cm2/h through Caucasian skin. The cumulative mass of platinum permeated after 24 hours of exposure was statistically significantly (p = 0.044) higher through African skin (37.52 ng/cm2) than Caucasian skin (5.05 ng/cm2). The retention of platinum in African skin (3 064.13 ng/cm2) was more than twice the mass retained in Caucasian skin (1 486.32 ng/cm2).
Conclusions: The in vitro diffusion method is an applicable method to determine skin permeability of metals. However, the experimental design and format of data reporting should be standardised to enable comparison of results from different studies. Platinum and rhodium permeated through intact human skin, with platinum permeation significantly higher. African skin was significantly more permeable by platinum than Caucasian skin. Both platinum and rhodium were retained inside the skin after 24 hours of exposure, possibly forming a reservoir which could contribute to continued permeation through the skin even after removal thereof from the skin. Platinum and rhodium permeated through full thickness skin and thereby could possibly contribute to local skin symptoms such as dermatitis and urticaria found in occupationally exposed workers. By permeating through the upper layers of the skin, these metals could potentially reach the viable epidermis and contribute to sensitisation. / PhD (Occupational Hygiene), North-West University, Potchefstroom Campus, 2015
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Modelling the evolution of pulsar wind nebulae / Michael Johannes VorsterVorster, Michael Johannes January 2014 (has links)
This study focusses on modelling important aspects of the evolution of pulsar wind nebulae
using two different approaches. The first uses a hydrodynamic model to simulate the morphological
evolution of a spherically-symmetric composite supernova remnant that is expanding
into a homogeneous interstellar medium. In order to extend this model, a magnetic field is
included in a kinematic fashion, implying that the reaction of the fluid on the magnetic field
is taken into account, while neglecting any counter-reaction of the field on the fluid. This approach
is valid provided that the ratio of electromagnetic to particle energy in the nebula is
small, or equivalently, for a large plasma β environment. This model therefore allows one to
not only calculate the evolution of the convection velocity but also, for example, the evolution
of the average magnetic field.
The second part of this study focusses on calculating the evolution of the energy spectra of
the particles in the nebula using a number of particle evolution models. The first of these is
a spatially independent temporal evolution model, similar to the models that can be found
in the literature. While spatially independent models are useful, a large part of this study
is devoted to developing spatially dependent models based on the Fokker-Planck transport
equation. Two such models are developed, the first being a spherically-symmetric model that
includes the processes of convection, diffusion, adiabatic losses, as well as the non-thermal
energy loss processes of synchrotron radiation and inverse Compton scattering. As the magnetic
field geometry can lead to the additional transport process of drift, the previous model is
extended to an axisymmetric geometry, thereby allowing one to also include this process. / PhD (Space Physics), North-West University, Potchefstroom Campus, 2014
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Modelling the evolution of pulsar wind nebulae / Michael Johannes VorsterVorster, Michael Johannes January 2014 (has links)
This study focusses on modelling important aspects of the evolution of pulsar wind nebulae
using two different approaches. The first uses a hydrodynamic model to simulate the morphological
evolution of a spherically-symmetric composite supernova remnant that is expanding
into a homogeneous interstellar medium. In order to extend this model, a magnetic field is
included in a kinematic fashion, implying that the reaction of the fluid on the magnetic field
is taken into account, while neglecting any counter-reaction of the field on the fluid. This approach
is valid provided that the ratio of electromagnetic to particle energy in the nebula is
small, or equivalently, for a large plasma β environment. This model therefore allows one to
not only calculate the evolution of the convection velocity but also, for example, the evolution
of the average magnetic field.
The second part of this study focusses on calculating the evolution of the energy spectra of
the particles in the nebula using a number of particle evolution models. The first of these is
a spatially independent temporal evolution model, similar to the models that can be found
in the literature. While spatially independent models are useful, a large part of this study
is devoted to developing spatially dependent models based on the Fokker-Planck transport
equation. Two such models are developed, the first being a spherically-symmetric model that
includes the processes of convection, diffusion, adiabatic losses, as well as the non-thermal
energy loss processes of synchrotron radiation and inverse Compton scattering. As the magnetic
field geometry can lead to the additional transport process of drift, the previous model is
extended to an axisymmetric geometry, thereby allowing one to also include this process. / PhD (Space Physics), North-West University, Potchefstroom Campus, 2014
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The effects of culture, values, diffusion of innovation and technology acceptance on attitudes towards digital banking adoption / Uitwerking van kultuur, waardes, die diffusie van innovering en die aanvaarding van tegnologie op ingesteldhede rakende die aanvaarding van digitale bankwese / Umphumela wosiko, amagugu, ukusabalala kobuqambi Kanye nokwamukelwa kwetheknoloji kwimibono mayelana nokusebenzisa ibhangi ngendlela yedijithali / Efeito da cultura, valores, aceitacao de tecnologias e difusao da inovacao nas atitudes em relacao a banca electronica / Vuyelo ra ndhavuko, mikhuva, ku amukeriwa ka thekinoloji ni ku haxiwa ka switirhisiwa swinswha eka malangutelo yo yelana ni ku tirhisa bangi ya internetMacamo, Monica Aniceto 08 1900 (has links)
Abstracts in English, Afrikaans, Zulu, Tsonga and Portuguese / The purpose of the study was to develop a model of attitudes toward digital banking, by exploring the relationship between values, culture, the diffusion of innovation and the Technology Acceptance. In addition, actual behaviour in the use of digital banking, as well as the moderating effects of gender, age, education level, income, and urban versus rural backgrounds, was included to provide additional insights into the financial services market in Maputo, Mozambique. A quantitative survey with a convenience sample (n = 403) of bankable individuals (male and female) between the ages of 21 and 60, was conducted from 2015–2018. The findings revealed significant relationships between the variables included in the empirical model. Structural equation modelling indicated that the data were a good fit, resulting from the use of a correlations-derived measurement model. These findings provide new insights into the development of tailor-made digital banking actions to drive usage in different segments, which will contribute to the body of knowledge on consumer behaviour and digital banking adoption and use. / Hierdie studie se doel was om ’n model van ingesteldhede teenoor digitale bankwese te ontwikkel deur ondersoek in te stel na die verband tussen waardes, kultuur, die diffusie van innovering en die aanvaarding van tegnologie. Werklike gedrag wanneer digitale bankwese gebruik word, asook die modererende effek van gender, ouderdom, onderwysvlak, inkomste en stedelike teenoor plattelandse agtergrond, is ingesluit om bykomende insigte te verkry in die finansiëledienstemark in Maputo, Mosambiek. ’n Kwantitatiewe opname met ’n gerieflikheidsteekproef (n = 403) van bankbare individue (manlik en vroulik) tussen die ouderdomme 21 tot 60, is in die tydperk 2015 tot 2018 onderneem. Die bevindinge toon dat daar beduidende verbande is tussen die veranderlikes wat by die empiriese model ingesluit word. Die modellering van strukturele vergelykings toon dat die data goed gepas het omdat ’n korrelasie-afgeleide metingsmodel gebruik is. Hierdie bevindinge lei tot nuwe insigte in die ontwikkeling van pasgemaakte digitalebankwese-aksies om gebruik in verskillende segmente aan te dryf. Dit sal bydra tot die bestaande kennis van verbruikersgedrag en die aanvaarding en gebruik van digitale bankwese. / Inhloso yalolu cwaningo bekuwukwenza imodeli yendlela yokubona mayelana nokusebenzisa ibhangi ngendlela yedijithali, ukuhlola ubudlelwane phakathi kwama-value, usiko, ukusabalala kobuqambi kanye nokwamukelwa kwetheknoloji. Nangaphezu kwalokho, indlela yokuziphatha ekusebenziseni ibhangi ngendlela yedijithali, kanye nemiphumela ethobayo yobulili, iminyaka yobudala, izinga lemfundo, ingeniso, kanye nokuqhathanisa abasemadolobheni nabasemakhaya, kwabandakanywa ukuhlinzeka ngemibono engezelelekile kwimakethe yamasevisi ezezimali eMaputo eMozambique. Kwenziwe inhlolovo ngobuningi ngesampuli ye-convenience (n = 403) wabantu abasebenzisa ibhangi (abesilisa nabesimame) abaphakathi kweminyaka engu 21 kanye nengu 60 kwenziwe ukusukela ku 2015–2018 Imiphumela iveza ubudlelwane obubalulekile phakathi kwezinto ezehlukene ezibandakanya imodeli yobufakazi obubambekayo. I-structural equation modelling ikhombise ukuthi ulwazi belungoluhambisana kahle, ngokuvela kumphumela wokusebenzisa i-correlations-derived measurement model. Okutholakele kunikeza imibono emisha ngokukuthuthukiswa kwezinyathelo zokusebenzisa ibhangii ngendlela yedijithali okuhlinzekelwa abantu ngokuhambisana nabo, ukuphakamisa ukusetshenziswa ngabantu bemikhakha ehlukene, lokhu okuthela esivivaneni kumthamo wolwazi ngendlela yokuziphatha kwabasebenzisi, kanye nokwamukelwa kokusebenzisa ibhangi ngendlela yedijithali kanye nokusetshenziswa. / O presente estudo tinha como objectivo desenvolver um modelo de atitudes em relação à banca electrónica explorando a relação entre os Valores, a Cultura e a Difusão da Inovação e Aceitação de Tecnologias. Além disso, o comportamento real relacionado com o uso de serviços da banca electrónica, assim como o efeito moderador do género, idade, nível de escolaridade, rendimentos e os contextos urbano versus rural foram incluídos para permitir melhor compreensão do mercado de serviços financeiros de Moçambique (Maputo). De 2015 a 2018, fez-se um inquérito quantitativo em uma amostra de conveniência (n = 403) de indivíduos bancáveis (masculinos e femininos) com idades compreendidas entre os 21 e os 60 anos. Os resultados revelaram relações significativas entre as variáveis incluídas no modelo empírico. A modelagem de equações estruturais indicou um bom número de dados, o que resultou num modelo canónico de medição derivada de correlações. Ademais, faz-se uma exposição e interpretação de achados sensíveis sob o ponto de vista ético no contexto de banca electrónica baseado no consumidor. Estes achados servirão de base para um novo conhecimento para o desenvolvimento de acções de banca electrónica personalizadas com vista a incentivar o uso em diferentes segmentos, o que acrescentará valor ao conhecimento actual sobre o Comportamento do Consumidor e a utilização de serviços de banca electrónica. / Xikongomelo xa dyondzo leyi a ku ri ku hlamusela malangutelo ya vanhu ehenhla ka ku tirhisiwa ka bangi ya internet hi ku kambisisa vuxaka lebyi nga kona exikarhi ka Mikhuva, Ndhavuko ni ku Haxiwa ka Switirhisiwa Swintswha ni leswi thyiwaka ku amukeriwa ka thekinoloji. Ku engetela kwalaho, ku katsiwile mikhuva ya vhanu ehenhleni ka ku tirhisa bangi ya internet, ku katsa ni vuyelo leri ringaniselaka ro va munhu a ri wanuna kumbe wansati, ntanga, dyondzo, muholo, ni leswaku u tshama dorobeni kumbe emakaya, leswaku ku twisisiwa hi vuenti leswaku mintirho ya swa timali yi tshamise ku yini eMozambique (Maputo). Nxaxamelo wa swivutiso swo teka tinhlayo wu tirhisiwile eka ntlawa lowu voniweke wu ringana (n = 403) wa vanhu lava nga tirhisaka bangi (va xinuna ni va xisati), lava nga ni 21 ku ya ka 60 wa malembe ku sukela 2017 ku ya ka 2018. Tinhlamulo ti kombe leswaku ku ni ku yelana lokukulu exikarhi ka swivutiso leswi katsiweke eka xivumbeko xo hlamusela ha xona. Structural equation modelling yi kombe leswaku ku ni mihandzu yo tala, leswi endleke leswaku ku va ni xivumbeko lexi se xi tiviwaka xa mpimo lowu taka hi ka ku yelana ka swilo (correlations-derived measurement model). Mihandzu leyi yelanaka ni swilo leswi khumbaka mahanyela lamanene leswi lavaka ku voniwa hi vukheta na yona ya vikiwa yi tlhela yi hlamuseriwa hi ku landza matirhelo ya bangi ya internet hi ku ya vatirhisi va yona. Mihandzu leyi yi ta tisa vutisi byintswha leswaku ku makiwa mintirho ya bangi ya internet leyi tivaka ku tsakisa mutirhisi leswaku ku engeteriwa ku tirhisiwa ka yona exikarhi ka mintlawa yo hambana, leswi na swona swi nga ta engetela vutivi ehenhleni ka Mikhuva ya Vatirhisi ni matirhiselo ya bangi ya internet. / Industrial and Organisational Psychology / Ph. D. (Industrial and Organisational Psychology)
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