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Cette thèse s’inscrit dans le domaine de l'opto-mécanique et propose l'utilisation de différentes techniques de mesure et de manipulation des propriétés mécaniques de nano-structures.La première partie de ce travail est dédiée aux fils photoniques. Ces objets sont des structures en GaAs en forme de cône inversé, avec une longueur d’une dizaine de µm et un diamètre inférieur au µm, contenant une couche de boîtes quantiques à l'intérieur. Nous avons démontré une méthode de réglage statique du spectre de photoluminescence de ces boîtes quantiques sensibles à la contrainte, en utilisant des nano-manipulateurs pour contraindre mécaniquement les fils. De plus, grâce à la dépendance spatiale du décalage spectral, il est possible d’établir une carte de la position des boîtes quantiques.La deuxième partie de ce travail concerne la mise en mouvement de ces fils photoniques à l’aide d’un faisceau laser modulé à la fréquence de résonance mécanique. Les mécanismes physiques à l’origine de ces effets sont présentés et discutés.Dans la troisième partie, nous présentons une méthode permettant l’observation d'oscillations mécaniques de nano-fils fins (moins de 50 nm de diamètre) en utilisant un microscope électronique à balayage. Cette méthode originale offre la possibilité de contrôler de nombreux types de structures micro et nano-électromécaniques, dont la détection du mouvement n’est pas possible optiquement en raison de la limite de diffraction de la lumière. De plus, cette méthode permet également d'agir sur les propriétés mécaniques des structures via une force de contre-réaction qui devient non négligeable pour ces structures très légères. Cela ouvre la possibilité d'études fondamentales complémentaires liées au refroidissement du mouvement mécanique. / This thesis is related to the field of opto-mechanics and the use of different techniques for the measurement and manipulation of mechanical properties of nano-structures.First part of the work is dedicated to the photonic wires. These objects are GaAs structures with an inverted conical shape of length of the order of 10 µm and diameter of less than 1 µm, containing a layer of InAs quantum dots inside. Wide-range static stress-tuning of quantum dots photoluminescence spectrum was demonstrated using nano-manipulators to bend the wires. Additionally, owing to the spatial dependence of the spectral shift, this technique offers the possibility of QD positions mapping.The second part of this work concerns the optical actuation of these photonic wires. A laser beam focused on the wire and modulated at the mechanical resonance frequency can set the wire in motion. The physical mechanisms responsible for these effects are presented and discussed.In the third part is presented a method enabling the detection of mechanical oscillations of small (less than 50 nm in diameter) nanowires with the use of a Scanning Electron Microscope. This original method offers a possibility to detect the motion of many types of micro- and nano-electromechanical devices which are too small to be detected optically owing to light diffraction limit.Moreover, this method also affects the mechanical properties of the structures via a back-action force that becomes non-negligible for such small devices. It opens up the possibility for further fundamental studies related to cooling of the mechanical motion.

Made available in DSpace on 2015-03-26T13:46:35Z (GMT). No. of bitstreams: 1 texto completo.pdf: 729175 bytes, checksum: d8be6fdfabfabf889bd81cc7f09142f9 (MD5) Previous issue date: 2008-05-16 / The Rhipicephalus (Boophilus) microplus is one of the most important arthropods in veterinary medicine due economic losses and health problems caused in cattle production, mainly in Central and South America, as well as in Australia. As an alternative to chemical control, immunization of bovines with Bm86 antigen to induce a protective immune response. A synthetic peptide, SBm 7462, derived from Bm86, has been shown great results in control of ticks. The construction and synthesis of one nucleotide sequence based on this peptide might be useful for design a DNA vaccine that has many advances than peptide vaccine. A gene, called seq1, was constructed with a three repetition of nucleotide sequence of SBm 7462. It was cloned into a pCIneo vector expression in mammals, transfected in VERO cells and injected in BALB/c mouse. Two methods were used to analysis of peptide expression in vitro: DOT ELISA and PAP. In both, the results showed that the nucleotide sequence (seq1) had not been express in VERO cells. In vivo, when mice were inoculated with the expression cassette they did not response in ELISA. They elevated antibody titles only when vaccinated with the syntetic peptide SBm 7462. And, the best titles of immunoglobulins were seen when the SBm 7462 was administered subcutaneously. After that, we insert a mutation at the begging of seq1, but, the sequenciament demonstrated that any initiation codon (ATG) had been inserted. / O Rhipicephalus (Boophilus) microplus é um dos mais importantes artrópodes em medicina veterinária devido perdas econômicas e problemas de saúde causados na produção de gado na América Central e do Sul, bem como na Austrália. Como alternativa ao controle químico, a imunização de bovinos com antígeno proteíco Bm86 induz uma resposta imune protetora. Um peptídeo sintético, SBm 7462, derivado da Bm86, tem obtido excelentes resultados no controle de carrapatos. A construção e síntese de uma sequência nucleotidíca baseada neste peptídeo podem ser útil para o desenho de uma vacina de DNA que tem muitas vantagens sob uma vacina sintética. Um gene, denominado seq1, foi construído repetindo três vezes a sequência nucleotidídica do SBm 7462. Ele foi clonado no vetor de expressão em mamíferos, pCIneo, transfectado em células VERO e injetado em camundongos BALB/c. Dois métodos foram usados para análise da expressão do peptídeo in vitro: DOT ELISA e PAP. Em ambos, os resultados demonstraram que a seqüência nucleotídica (seq1) não havia sido expressa em células VERO. In vivo, quando camundongos foram inoculados com o cassete de expressão eles não responderam ao ELISA. Eles elevaram os títulos de anticorpos, apenas, quando inoculados com o peptídeo sintético SBm 7462. Os melhores títulos de imunoglobulinas foram vistos quando o SBm 7462 foi administrado subcutâneamente. Após isso, inserimos uma mutação no início do seq 1, porém, o sequenciamento demonstrou que nenhum códon de iniciação (ATG) tinha sido inserido.

Este trabalho visa investigar a resposta sorológica para glicolipoproteína (GLP) de leptospiras com a finalidade de padronizar o uso deste antígeno em testes sorológicos para o diagnóstico da leptospirose humana. Dentre as proteínas envolvidas na patogenicidade das leptospiras, a GLP ainda não foi estudada quanto a imunogenicidade e quanto ao seu emprego como antígeno na detecção de anticorpos específicos. Assim, dot-ELISA para detecção de anticorpos IgG e IgM, com o emprego de GLP de leptospiras patogênica e não patogênica, foi padronizado. Foram testadas amostras pareadas de soros de 90 pacientes com leptospirose confirmada sorologicamente por MAT, sendo as primeiras amostras colhidas na fase aguda da doença e as segundas amostras após 10 a 15 dias. Foram testadas também amostras únicas de 30 pacientes de diferentes patologias, que apresentaram resultados negativos no MAT, pertencentes ao grupo controle. A detecção de anticorpos IgG não mostrou resultados satisfatórios, tendo sido, o seu estudo, descontinuado. Os resultados do dot-ELISA IgM mostraram sensibilidade de 100% nas amostras colhidas após soroconversão no MAT e a precocidade de detecção foi demonstrada pela alta positividade nas amostras colhidas na fase aguda da doença (76,6% para dot-ELISA com antígeno de Leptospira interrogans sorovar Copenhageni e 90% com antígeno de Leptospira biflexa sorovar Patoc). A especificidade foi alta (96,6%), porém o dot-ELISA utilizando ambos os antígenos apresentou 3,3% de resultados falso-positivos. Este estudo demonstrou a importância da resposta imune humoral ao antígeno GLP de leptospiras. A utilização da GLP, no teste dot-ELISA para detecção de anticorpos IgM permitiu realizar o diagnóstico sorológico de forma simples, rápida e com alta eficiência diagnóstica. / The aim of this work was the study of serologic response against leptospira glycolipoprotein (GLP) for the purpose of standardizing its use as an antigen on serological tests to diagnose human leptospirosis. Among the proteins involved in the pathogenicity, GLP is yet to be studied regarding its immunogenicity and its use as an antigen in the detection of specific antibodies. That led to our standardization of dot-ELISA for detecting IgG and IgM antibodies, using GLP extracted from either pathogenic Leptospira interrogans serovar Copenhageni or nonpathogenic Leptospira biflexa serovar Patoc. Paired serum samples were taken from 90 patients with serologically confirmed leptospirosis, by MAT, with the first samples collected on the disease\'s acute phase and the second samples after a period of 10 to 15 days. We also tested single samples taken from 30 patients with other diseases, MAT negative, belonging to the control group. Detection rates for IgG antibodies were unsatisfactory, so the study for that use was discontinued. The dot-ELISA IgM results yielded 100% sensitivity on the samples taken after MAT seroconversion. The early detection pattern was evidenced by the high positivity rate on the samples taken during the disease\'s acute phase (76,6% dot-ELISA using Leptospira interrogans serovar Copenhageni antigen and 90% using Leptospira biflexa sorovar Patoc antigen). Specificity rates were high (96.6%), although a 3.3% rate of false-positive results was observed for dot-ELISA using both antigens. The present study revealed the importance of the humoral immune response against the GLP antigen. The use of GLP, on the dot-ELISA test for IgM antibodies afforded a simple, quick and effective diagnosis.

O Tomato chlorosis virus (ToCV) é uma espécie do gênero Crinivirus que causa danos, principalmente na cultura do tomateiro (Solanum lycopersicum). Foi primeiramente isolado e descrito em 1998, nos Estados Unidos, e em seguida foi reportado em doze países. No Brasil, foi constatado primeiramente no Estado de São Paulo, na região de Sumaré, em 2008, e posteriormente nos Estados da Bahia, Espírito Santo, Goiás, Minas Gerais e Rio de Janeiro. Há evidência da sua presença também nos Estados do Paraná e Santa Catarina. O ToCV pode infectar outras solanáceas além do tomateiro e, recentemente, foi observado infectando plantas de batata (Solanum tuberosum) no Brasil. Esse crinivirus é transmitido no Brasil principalmente pelo aleirodídeo (mosca branca) Bemisia tabaci MEAM1. Considerando o patossistema batateira/ToCV, não há estudos sobre a ocorrência, sintomatologia em diferentes variedades e danos provocados por esse crinivirus. Também não há antissoro policlonal para o isolado brasileiro do ToCV para uso na diagnose da doença em solanáceas. Esse trabalho teve por objetivos: purificar o ToCV e produzir antissoro policlonal; avaliar a reação de genótipos de batateira à infecção com o ToCV; avaliar o dano provocado por esse vírus em duas variedades de batateira. A purificação do vírus a partir de folhas de tomateiro e a produção de antissoro policlonal em coelho foram satisfatórias. No entanto, o antissoro não foi eficiente em ELISA, mas sim em dot-blot e somente na diluição de 1:20. Foi avaliada a reação de 21 genótipos de batateira à infecção com o ToCV, por meio da inoculação com B. tabaci MEAM1, com chance de escolha do vetor. Nenhum genótipo exibiu resistência à infecção; enquanto a variedade Camila foi assintomática e não apresentou alteração na fotossíntese. Plantas de batateira das variedades Ágata e Asterix sadias foram inoculadas com o ToCV, por meio da B. tabaci MEAM1 e ao final foram avaliadas a massa fresca da parte aérea, peso e número dos tubérculos colhidos. Em dois experimentos independentes, as reduções médias no peso fresco da parte aérea foram de 60,1% para Ágata e 46% para Asterix. Porém, as reduções nas produções dessas variedades, no primeiro experimento foram de 99,5% e 98,1%, respectivamente; enquanto no segundo os valores foram de 82,3% e 56,2%, respectivamente. / Tomato chlorosis virus (ToCV) is a species of the genus Crinivirus, which is causing considerable losses mainly on tomato crop (Solanum lycopersicum). It was first isolated and described on 1998 in the United States and subsequently reported in twelve countries. In Brazil, it was first reported in São Paulo State, in Sumaré region in 2008, and after that on the states of Bahia, Espírito Santo, Goiás, Minas Gerais and Rio de Janeiro. There is evidence of the presence of ToCV on the states of Paraná and Santa Catarina. ToCV can also infect other solanaceae and more recently, it was reported infecting potato plants (Solanum tuberosum) in Brazil. This crinivirus is transmitted by Bemisia tabaci MEAM1. Considering the patosystem potato/ToCV, there are no studies on the occurrence, symptomatology in different varieties, and damages caused by this crinivirus. In addition, there is no polyclonal antiserum for the Brazilian isolate of ToCV for use in diagnosis. The objectives of the present work were: to purify the virus and produce a polyclonal antiserum; to evaluate the reaction of potato genotypes to ToCV infection; to evaluate the yield loss caused by this crinivirus on two potato cultivars. The virus purification from tomato leaves and the production of polyclonal antiserum in rabbit were satisfactorily accomplished. However, the antiserum was not efficient on ELISA test, but in dot-blot, only when diluted 1:20. The reaction of 21 potato genotypes to infection with ToCV was evaluated by inoculation with B. tabaci MEAM1, with chance of choice for the vector. All genotypes were infected with ToCV and Camila was the only one asymptomatic. Plants of cultivars Ágata and Asterix were inoculated with ToCV, by means of viruliferous vector, and at the end were evaluated for the fresh mass of the aerial part, weight and number of harvested tubers. In two independent experiments, average reductions in aerial fresh weight were 60.1% for Ágata and 46% for Asterix. However, reductions in yield of these varieties in the first experiment were 99.5% and 98.1%, respectively; while in the second the values were 82.3% and 56.2%, respectively.

The cost effective comb based laser sources are considered to be one of the prominent emitters used in optical communication (OC) and photonic integrated circuits (PIC). With the rising demand for delivering triple-play services (voice, data and video) in FTTH and FTTP-based WDM-PON networks, metropolitan area network (MAN), and short-reach rack-to-rack optical computer communications, a versatile and cost effective WDM transmitter design is required, where several DFB lasers can be replaced by a cost effective broadband comb laser to support on-chip optical signaling. Therefore, high performance quantum dot (Q.Dot) comb lasers need to satisfy several challenges before real system implementations. These challenges include a high uniform broadband gain spectrum from the active layer, small relative intensity noise with lower bit error rate (BER) and better temperature stability. Thus, such short wavelength comb lasers offering higher bandwidth can be a feasible solution to address these challenges. However, they still require thorough characterization before implementation. In this project, we briefly characterized the novel quantum dot comb laser using duty cycle based electrical injection and temperature variations where we have observed the presence of reduced thermal conductivity in the active layer. This phenomenon is responsible for the degradation of device performance. Hence, different performance trends, such as broadband emission and spectrum stability were studied with pulse and continuous electrical pumping. The tested comb laser is found to be an attractive solution for several applications but requires further experiments in order to be considered for photonic intergraded circuits and to support next generation computer-communications.

We will introduce three new classes of graphs; namely bipartite dot product graphs, probe dot product graphs, and combinatorial orthogonal graphs. All of these representations were inspired by a vector representation known as a dot product representation. Given a bipartite graph G = (X, Y, E), the bipartite dot product representation of G is a function ƒ : X ∪ Y → Rk and a positive threshold t such that for any κ ∈ Χ and γ ∈ Υ , κγ ∈ ε if and only if f(κ) · f(γ) ≥ t. The minimum k such that a bipartite dot product representation exists for G is the bipartite dot product dimension of G, denoted bdp(G). We will show that such representations exist for all bipartite graphs as well as give an upper bound for the bipartite dot product dimension of any graph. We will also characterize the bipartite graphs of bipartite dot product dimension 1 by their forbidden subgraphs. An undirected graph G = (V, E) is a probe C graph if its vertex set can be parti-tioned into two sets, N (nonprobes) and P (probes) where N is independent and there exists E' ⊆ N × N such that G' = (V, E ∪ E) is a C graph. In this dissertation we introduce probe k-dot product graphs and characterize (at least partially) probe 1-dot product graphs in terms of forbidden subgraphs and certain 2-SAT formulas. These characterizations are given for the very different circumstances: when the partition into probes and nonprobes is given, and when the partition is not given. Vectors κ = (κ1, κ2, . . . , κn)T and γ = (γ1, γ2, . . . , γn)T are combinatorially orthogonal if |{i : κiγi = 0}| ≠ 1. An undirected graph G = (V, E) is a combinatorial orthogonal graph if there exists ƒ : V → Rn for some n ∈ Ν such that for any u, υ &Isin; V , uv ∉ E iff ƒ(u) and ƒ(v) are combinatorially orthogonal. These representations can also be limited to a mapping g : V → {0, 1}n such that for any u,v ∈ V , uv ∉ E iff g(u) · g(v) = 1. We will show that every graph has a combinatorial orthogonal representation. We will also state the minimum dimension necessary to generate such a representation for specific classes of graphs.

Cette thèse a été consacrée à l'étude théorique et expérimentale du couplage contrôlé de la source de photon unique (SPS) aux structures photoniques multidimensionnelles, par l'utilisation de la méthode dite écriture directe par laser (DLW) par absorption ultra-faible à un photon (LOPA). La thèse est constitutée de trois parties principales suivantes:La première partie concerne le caractérisation et l'optimisation des dots quantiques (QD) colloïdaux CdSe/CdS. La dépendance de la longeur d'onde d'excitation a été étudiée. En utilisant une excitation à 532 nm, seul le core est excité et l’effet d'Auger est donc supprimé. Cette approche permet donc d'obtenir avec la suppression de la commutation intermittente et une source de photon unique très stable à température ambiante. Afin d’obtenir une meilleure performance des QDs sur une longue période, nous avons ensuite étudié l'influence du milieu dans lequel les QDs sont logés sur leurs propriétés optiques. En intégrant les QDs dans les matrices de polymère SU-8, nous avons montré que l'environnement polymérique permet non seulement de conserver de bonnes caractéristiques des QDs CdSe/CdS avec une photostabilité élevée, mais également de nous offrir une excellente accessibilité à la fabrication des structures en polymère contenant une particule unique.Dans la deuxième partie, la technique LOPA DLW est utilisée pour le couplage des QDs uniques dans diverses structures photoniques. Deux dispositifs, l’antenne du type ''pilier diélectrique'' sous-lambda et la membrane de réseau circulaire (cavité du type ''bulleye''), ont été étudiés théoriquement et expérimentalement pour améliorer l’émission du QD couplé en termes de l'émission spatiale et de l’émission radiative spontanée de l’émetteur.Dans la troisième partie, la manipulation de la SPS est démontrée en couplant le QD unique à des structures magnéto-photoniques multidimensionnelles. À l'aide d'un champ magnétique externe, le mouvement contrôlable d'un seul QD a été démontré dans un environnement fluidique. En contrôlant l'amplitude et l'orientation du champ magnétique externe, la position et l'orientation de la SPS à base d'un QD ont été manipulées à la demande. Les propriétés optiques, magnétiques et mécaniques des dispositifs magnéto-photoniques hybrides ont été étudiées pour montrer les capacités multifonctionnelles de telles structures. / The thesis has been devoted to study the controlled coupling of a colloidal quantum dot (QD) based single photon source (SPS) into multidimensional polymeric photonic structures by using low-one photon absorption (LOPA) direct laser writing (DLW) technique. The thesis consists of three main parts:The first part addresses the characteristic optimization of the CdSe/CdS based SPS. The excitation wavelength dependence of the QDs was investigated. By using 532 nm, only the core of the QD is excited with the suppression of the Auger effects. Thus, this approach allows for obtaining the suppression of fluorescence intermittency and a stable single-photon emission at ambient conditions. In order to obtain the long-term high fluorescence quality of the QDs, we then studied the influence of the local dielectric medium on the optical properties of the QDs. By incorporating the QDs into a photoresist (SU-8), we demonstrated that the polymeric environment not only enables the long-term preservation of the QD with high photostability but also provides us excellent accessibility to fabricate polymeric structures containing SPS.In the second part, the LOPA-based DLW is employed for the coupling of single QD into various photonic structures. Two devices including submicropillar dielectric antenna and 3D membrane bulleye cavity are theoretically and experimentally investigated to enhance the fluorescence emission of the single QD in terms of far-field angular radiation pattern and the spontaneous radiative emission of the emitter.In the third part, the manipulation of SPS is demonstrated by coupling the single QD into multidimensional magneto-photonic structures. With the aid of an external magnetic field, the controllable movement of the coupled QD was performed in the fluidic environment. The position and orientation of the SPS coupled in the structure were manipulated on demand. The mechanical, magnetic and optical properties of the device are investigated showing the multifunctional capabilities of magneto-photonic structures.

Partial discharge (PD) is a micro discharge that occurs in defected regions within the insulating media. As these discharges are the main culprits that cause dielectric material aging, PD measurements have been used for assessing insulating materials, including solids, liquids, and gases for power applications. There are various methods and sensors available for measuring PD sensitive to specific characteristics and operable over a wide range of frequencies. Most PD measurement techniques provide patterns that enable PD interpretation more comfortable for users. For example, in AC applications, the phase-resolved partial discharge (PRPD) technique provides identifiable patterns for distinguishing various types of PDs. However, the establishment of meaningful patterns to multiple types of PD in DC systems requires more sensitive and accurate measurements of individual PD pulses with noise rejection functionality due to the lack of phase-resolved information. Investigating of the transient phenomena such as individual PD pulses requires well-designed circuits with sufficiently large bandwidths. Waveshapes can be easily disturbed by background noise and deformed by the frequency response of measuring circuits and data acquisition systems (DAQ). Noises are unwanted disturbances that could be suppressed by suitable filters or mathematical methods. Measurement circuits and DAQ systems consist of transmission lines, sensors, cables, connectors, DAQ hardware, and oscilloscopes. Therefore, matching the impedance of all components guarantees a reflectionree path for traveling signals and addresses most of the challenges relevant to transient measurements. In this dissertation, we proposed and designed an appropriate testbed equipped with high bandwidth transmission line and electromagnetic field sensors suitable for investigating PD under DC electrical stresses. We comprehensively used finite element analysis simulations through the COMSOL Multiphysics software to design the dimensions and evaluate the frequency response of the testbed, transmission line, and electromagnetic sensors. Furthermore, based on the new testbed, DC PD measurements were performed using conventional and non-conventional sensors. Finally, various types of DC PD were statistically classified based on the proposed testbed.

No description available.