KwaGandaganda is an Early Iron Age (EIA) site in the .Mngeni valley (KwaZulu-Nata~ South Africa). Three cultural phases, Msuluzi, Ndondondwane and Ntshekane, dating between AD 620- AD 1030 were identified. The objective was to establish the extent of animal exploitation during the EIA in KwaZulu-Natal by means of faunal analysis, using internationally accepted methods. The collection included 41 006 fragments of which 22.9% were identifiable. A large number of species (68) were identified, including Rattus rattus. The collection yielded the largest quantity of ivory chips ever found on an EIA site in southern Africa, as well as an extensive variety of pathological specimens, mostly from Bos taurus. Several possible divining bones were present in the sample. Herd management, hunting strategies, gathering activities, fishing and trading of animal goods during the EIA were discussed, while the consequences of the unique excavation methods (i.e. the use of bulldozers) were also commented upon. / Anthropology and Archaeology / M.A. (Anthropology)

The mechanisms of pre-mRNA splicing regulation are poorly understood. Here we dissect how the Saccharomyces cerevisiae ribosomal L30 protein blocks splicing of its pre-mRNA upon binding a kink-turn structure including the 5' splice site. We show that L30 binds the nascent RPL30 transcript without preventing recognition of the 5' splice site by U1 snRNP but blocking U2 snRNP association with the branch site. Interaction of the factors BBP and Mud2p with the intron, relevant for U2 snRNP recruitment, is not affected by L30. Furthermore, the functions of neither the DEAD-box protein Sub2p in the incipient spliceosome, nor of the U2 snRNP factor Cus2p on branch site recognition, are required for L30 inhibition. These findings contrast with the effects caused by binding a heterologous protein to the same region, completely blocking intron recognition. Collectively, our data suggest that L30 represses a spliceosomal rearrangement required for U2 snRNP association with the nascent RPL30 transcript.

The informational content of 3' splice sites is low and the mechanisms whereby they are selected are not clear. Here we enunciate a set of rules that govern their selection. For many introns, secondary structures are a key factor, because they occlude alternative 3'ss from the spliceosome and reduce the effective distance between the BS and the 3'ss to a maximum of 45 nucleotides. Further alternative 3'ss are disregarded by the spliceosome because they lie at 9 nucleotides or less from the branch site, or because they are weak splice sites. With these rules, we are able to explain the splicing pattern of the vast majority of introns in Saccharomyces cerevisiae. When in excess, L30 blocks the splicing of its own transcript by interfering with a critical rearrangement that is required for the proper recognition of the intron 3' end, and thus for splicing to proceed. We show that the protein Cbp80 has a role in promoting this rearrangement and therefore antagonizes splicing regulation by L30. / Tanto la información que define el sitio de splicing 3' como los mecanismos de selección del mismo son poco conocidos. En este trabajo, proponemos una serie de reglas que gobiernan esta selección. Las estructuras secundarias son claves en el caso de muchos intrones, porque son capaces de ocultar sitios de splicing alternativos 3' al spliceosoma, y además reducen la distancia efectiva entre el punto de ramificación y el sitio de splicing 3' a un máximo de 45 nucleotidos. Otros sitios de splicing alternativo 3' no son considerados por el spliceosoma como tales porque se encuentran a 9 nucleotidos o menos del punto de ramificación, o porque son sitios de splicing débiles. Con estas reglas somos capaces de explicar el splicing de la mayoría de intrones de Saccharomyces cerevisiae. El exceso de proteína L30 bloquea el splicing de su propio tránscrito porque interfiere con la reorganización necesaria para el correcto reconocimiento del 3' final del intrón, y por tanto de su splicing. Demostramos que la proteína Cbp80 está implicada en promover esta reorganización y que por tanto antagoniza la regulación del splicing por L30.

The city of Wellington has a high population concentration and lies within a geologically active landscape at the southern end of the North Island, New Zealand. Wellington has a high seismic risk due to its close proximity to several major fault systems, with the active Wellington Fault located in the north-western central city. Varying soil depth and properties in combination with the close proximity of active faults mean that in a large earthquake rupture event, ground shaking amplification is expected to occur in Thorndon, Te Aro and around the waterfront. This thesis focuses on the area bounded by Thorndon Overbridge in the north, Wellington Hospital in the south, Kelburn in the west, and Oriental Bay in the east. It includes many of the major buildings and infrastructural elements located within the central Wellington commercial area. The main objectives were to create an electronic database which allows for convenient access to all available data within the study area, to create a 3D geological model based upon this data, and to define areas of different seismic subsoil class and depth to rock within the study area at a scale that is useful for preliminary geotechnical analysis (1:5,000. Borelogs from 1025 holes with accompanying geological and geotechnical data obtained from GNS Science and Tonkin & Taylor were compiled into a database, together with the results from SPAC microtremor testing at 12 sites undertaken specifically for this study. This thesis discusses relevant background work and defines the local Wellington geology. A 3D geological model of the central Wellington commercial area, along with ten ArcGIS maps including surficial, depth to bedrock, site period, Vs30, ground shaking amplification hazard and site class (NZS 1170.5:2004) maps were created. These outputs show that a significant ground shaking amplification risk is posed on the city, with the waterfront, Te Aro and Thorndon areas most at risk.

Este trabalho tem por objetivo investigar o significado das obras de arte pública permanentes, comissionadas pela 5.ª Bienal do Mercosul, em Porto Alegre, entre 2004 e 2006, sob o prisma das questões da site-specificity e/ou place-specificity. As esculturas de Mauro Fuke, José Resende, Carmela Gross e Waltercio Caldas, são tomadas como estudo de caso para a discussão dessas questões, ou seja, para investigar as possibilidades de transformação de um local (espaço físico) em lugar (local dotado de significados), por meio de obras de arte projetadas especificamente com esse objetivo. / The paper aims at searching for the meaning of perennial public artworks done under the 5th Mercosul Biennial (Porto Alegre, 2004-2006) order, looking at the issues of site-specificity and/or place-specificity. Mauro Fuke, José Resende, Carmela Gross and Waltercio Caldas’s sculptures are the case study for the debate of such issues. Investigating the possibility of transforming spaces into sites through the placement of artworks produced for that purpose.

KwaGandaganda is an Early Iron Age (EIA) site in the .Mngeni valley (KwaZulu-Nata~ South Africa). Three cultural phases, Msuluzi, Ndondondwane and Ntshekane, dating between AD 620- AD 1030 were identified. The objective was to establish the extent of animal exploitation during the EIA in KwaZulu-Natal by means of faunal analysis, using internationally accepted methods. The collection included 41 006 fragments of which 22.9% were identifiable. A large number of species (68) were identified, including Rattus rattus. The collection yielded the largest quantity of ivory chips ever found on an EIA site in southern Africa, as well as an extensive variety of pathological specimens, mostly from Bos taurus. Several possible divining bones were present in the sample. Herd management, hunting strategies, gathering activities, fishing and trading of animal goods during the EIA were discussed, while the consequences of the unique excavation methods (i.e. the use of bulldozers) were also commented upon. / Anthropology and Archaeology / M.A. (Anthropology)

Este trabalho tem por objetivo investigar o significado das obras de arte pública permanentes, comissionadas pela 5.ª Bienal do Mercosul, em Porto Alegre, entre 2004 e 2006, sob o prisma das questões da site-specificity e/ou place-specificity. As esculturas de Mauro Fuke, José Resende, Carmela Gross e Waltercio Caldas, são tomadas como estudo de caso para a discussão dessas questões, ou seja, para investigar as possibilidades de transformação de um local (espaço físico) em lugar (local dotado de significados), por meio de obras de arte projetadas especificamente com esse objetivo. / The paper aims at searching for the meaning of perennial public artworks done under the 5th Mercosul Biennial (Porto Alegre, 2004-2006) order, looking at the issues of site-specificity and/or place-specificity. Mauro Fuke, José Resende, Carmela Gross and Waltercio Caldas’s sculptures are the case study for the debate of such issues. Investigating the possibility of transforming spaces into sites through the placement of artworks produced for that purpose.

Este trabalho tem por objetivo investigar o significado das obras de arte pública permanentes, comissionadas pela 5.ª Bienal do Mercosul, em Porto Alegre, entre 2004 e 2006, sob o prisma das questões da site-specificity e/ou place-specificity. As esculturas de Mauro Fuke, José Resende, Carmela Gross e Waltercio Caldas, são tomadas como estudo de caso para a discussão dessas questões, ou seja, para investigar as possibilidades de transformação de um local (espaço físico) em lugar (local dotado de significados), por meio de obras de arte projetadas especificamente com esse objetivo. / The paper aims at searching for the meaning of perennial public artworks done under the 5th Mercosul Biennial (Porto Alegre, 2004-2006) order, looking at the issues of site-specificity and/or place-specificity. Mauro Fuke, José Resende, Carmela Gross and Waltercio Caldas’s sculptures are the case study for the debate of such issues. Investigating the possibility of transforming spaces into sites through the placement of artworks produced for that purpose.

Local site conditions are known to influence ground motion during earthquake events and increase the severity of damage. Data from earthquakes are useful to study the response but they are available only from active regions. Ubiquitous ambient vibrations on the other hand offer a more practical approach to quantify site responses. This thesis explores the use of various methods for obtaining site responses. The primary area of study is the Kachchh rift basin, NW India, a Mesozoic rift that features significant lateral variations in surface geology and has experienced ground responses during 1819 and 2001 earthquakes. The Mw 7.6, 2001 event was followed by hundreds of aftershocks, which were recorded by temporary networks. In this study we have used earthquake signals as well as ambient vibrations to understand site response in various parts of the basin. In addition we have collected data from a few sites from the Indo-Gangetic plains and Kathmandu valley, both affected by large earthquakes, 1934 the M ~ 8 (Bihar) and 2015, Mw 7.8 (Nepal). Velocity and acceleration records from a network of eight stations in the Kachchh Rift were used to evaluate site responses using Standard Spectral Ratio (SSR) and Horizontal to Vertical spectral ratio (HVSR-E) methods. Ambient vibrations were analyzed following Nakamura’s H/V method (HVSR-AV), for data collected from 110 sites that represent different field conditions within the Kachchh Rift. Fundamental resonance frequency (f0) varied between 0.12 – 2.30 Hz, while the amplification factor (A0) was in the range of 2.0 – 9.1. We found that higher A0 and liquefaction index (Kg) values were mostly associated with higher liquefaction potential. Using a close network of stations, we studied the role of site response in damage to the Bhuj city that suffered maximum damage in 2001; our results suggest that site response was not a significant factor. Studies based on passive data were complemented by Multi-channel Analysis of Surface Waves (MASW) to map shear wave velocities of the various subsurface units up to depths of 10m (Vs10) and 30m (Vs30). Our results imply average Vs could be a good proxy to characterize site amplifications where sediment thicknesses are shallow. Power law relationship between f0 and thickness (h) suggest a strong positive correlation (r = 0.89) adding credence to HVSR-AV method, making it a cost-effective alternative to MASW to infer site conditions. Further, to understand the influence of topography on site effects, we analyzed data from hills, valleys and their edges, both from the Kachchh rift and Kathmandu valley. Sites on the edges of valleys showed multiple, fuzzy peaks in the low frequency range (< 1 Hz) and broad peaks attributable to sites prone to higher damage. Spectrograms generated through Huang-Hilbert Transforms (HHT) suggested focusing of energy in narrow frequency bands on the edges, while valleys tend to scatter energy over wide frequencies. Although our current results are based on limited observations, we recognize spectral analysis as a powerful tool to quantify site effects in regions with significant topography. It is known that coseismic liquefaction could lead to nonlinear behavior wherein the near-surface soil layer loses its shear strength, causing a reduction of its fundamental resonance frequency. We used data from selected sites of coseismic liquefaction to highlight the significance of nonlinear effects in site response. Earthquake signals and ambient vibrations from Umedpur, a region that experienced intense liquefaction during 2001 were used in this analysis. Here we followed an empirical decomposition method based on HHT and signals were decomposed as many intrinsic mode functions (IMFs) that showed characteristic peaks for events of various values of PGAs. Thus, the first IMF for events with relatively higher PGAs (0.03g) showed distinct peaks for the S wave coda part, which were not noted for those with lower PGA (0.01g). These observations in a region of coseismic liquefaction are useful in developing models for quantifying nonlinear behavior. In conclusion, site response studies using different types of data and processing techniques in regions affected by recent earthquakes brings out the scope and limitations of each of these sets of data and techniques. This study suggests that ambient vibrations provide reasonable estimates of site response and can be reliably used in regions where earthquake data are not available.

In the mid-19th century, the Fort Vancouver employee Village was one of the most diverse settlements on the Pacific Coast. Trappers, tradesmen, and laborers from Europe, North America, and Hawaii worked and lived within a highly stratified colonial social structure. Their homes have been the site of archaeological research for nearly 50 years, but the architectural features and artifacts have received limited attention. Inspired by an 1845 description of the Village that described houses that were "as various in form" as their occupants (Hussey 1957:218), this study examined community-level social relationships in this 19th-century fur trade community through vernacular architecture and landscape. This thesis presents the life histories and layouts of five Village houses. The architectural analysis relied on data from features, square nails, window glass, and bricks. The resulting architectural interpretations were synthesized to explore the larger vernacular landscape of the Village and investigate whether the house styles reflect processes of creolization and community development, or distinction and segregation among the Village residents. The houses all stem from a common French-Canadian architectural tradition, built by the first employees at Fort Vancouver, but the life histories also revealed that the houses were occupied (and repaired) by a second wave of employees at some time during the 1840s. A reminder that Village houses deposits may reflect multiple owners, and should not be conceptualized as the result of a single household. Finally, this thesis demonstrates that nuanced architectural data that can yet be learned from past excavation assemblages when the many nails, bricks, and window glass specimens are reanalyzed using current methods.