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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
161

Bytový dům / Apartment house

Doležal, Ondřej January 2014 (has links)
The main theme of this Master´s thesis is a project of detached residential house of a terraced type, partially located in terrain. The building has three floors and a basement and is covered by a flat roof. There are six residential units in the house and three parking spaces, including technical support. The house will be built in the village of Pribyslavice, on the plots with parcel numbers 140/1 and 145/1. The ground plan of the house is of a rectangular shape. The vertical load-bearing structural system is chosen to be made from bricks. Horizontal system is made by using Spiroll prestressed panels. The internal staircase is made from concrete, monolithic. The material and construction solutions is chosen to be close to passive standards. The project fully respects currently applicable standards and technical regulations of individual manufacturers.
162

Vliv provedení zateplení bytového domu na výdaje spojené s provozem této nemovitosti / The impact of executing new thermal insulation on a apartment building on the expenses associated with its operation

Machová, Petra Unknown Date (has links)
The topic of the diploma thesis „The impact of executing new thermal insulation on a apartment building on the expenses associated with its operation “ is a draft and appraisal of four variants of thermal insulation of the building. The thesis deals with the assessment of the current proposal for apartment building envelopes along with proposing alternative plans with emphasis on reducing heating costs. The first section describes the types of buildings according to heating demand and a list of different variants of thermal insulation materials. The second part is devoted to a specific apartment building in the Ústí nad Labem for which there are designed in four variants of insulation. The work includes thermal assessment in original condition and the assessment of new condition modified by adding thermal insulation. Through research will bedetermined price of implementation of proposed insulation options together with the costs of operation of the apartment building. In conclusion, the diploma thesis evaluates the payback period of insulation.
163

Importance of substrate quality and clay content on microbial extracellular polymeric substances production and aggregate stability in soils

Olagoke, Folasade K., Bettermann, Antje, Nguyen, Phuong Thi Bich, Redmile-Gordon, Marc, Babin, Doreen, Smalla, Kornelia, Nesme, Joseph, Sørensen, Søren J., Kalbitz, Karsten, Vogel, Cordula 04 June 2024 (has links)
We investigated the effects of substrate (cellulose or starch) and different clay contents on the production of microbial extracellular polymeric substances (EPS) and concomitant development of stable soil aggregates. Soils were incubated with different amounts of montmorillonite (+ 0.1%, + 1%, + 10%) both with and without two substrates of contrasting quality (starch and cellulose). Microbial respiration (CO2), biomass carbon (C), EPS-protein, and EPS-polysaccharide were determined over the experimental period. The diversity and compositional shifts of microbial communities (bacteria/archaea) were analysed by sequencing 16S rRNA gene fragments amplified from soil DNA. Soil aggregate size distribution was determined and geometric mean diameter calculated for aggregate formation. Aggregate stabilities were compared among 1–2-mm size fraction. Starch amendment supported a faster increase than cellulose in both respiration and microbial biomass. Microbial community structure and composition differed depending on the C substrate added. However, clay addition had a more pronounced effect on alpha diversity compared to the addition of starch or cellulose. Substrate addition resulted in an increased EPS concentration only if combined with clay addition. At high clay addition, starch resulted in higher EPS concentrations than cellulose. Where additional substrate was not provided, EPS-protein was only weakly correlated with aggregate formation and stability. The relationship became stronger with addition of substrate. Labile organic C thus clearly plays a role in aggregate formation, but increasing clay content was found to enhance aggregate stability and additionally resulted in the development of distinct microbial communities and increased EPS production.
164

興櫃公司初次申請上市(櫃)(IPOs)股票蜜月現象及影響因素之研究

陳崇生, Chen, Boss Unknown Date (has links)
民國91年1月2日啟動交易的「興櫃市場」,提供投資者一個合法、安全及透明的未上市(櫃)股票交易市場,而依台灣證券交易所(TSEC)上市審查準則及櫃檯買中心(OTC)上櫃審查準則之規定欲申請初次申請上市(櫃)股票之公開發行公司,必先登錄興櫃股票,並掛牌交易達六個月以上,先熟悉市場交易規則及充分透明的資訊揭露,進而才有資格申請成為上市櫃公司。然興櫃市場是否具有「價格發現」機制,又影響其申請上市櫃(IPOs)股票蜜月現象因素為何,係本研究之探討重點。 本研究主要探討興櫃公司初次申請上市(櫃)(IPOs)股票蜜月現象及其影響因素,研究目的在於「暸解檢驗興櫃公司從興櫃登錄至申請上市(櫃)(IPOs)期間是否存在超額報酬,並從公司基本因素、公司於興櫃市場表現因素、公司上市(櫃)時條件因素、市場景氣與制度因素與公司治理因素等五大類,瞭解其對於IPOs蜜月現象的影響。此外,並以實際登錄興櫃公司申請上市櫃進度,從興櫃股票在登錄興櫃日、上市(櫃)送件日、上市(櫃)審議委員會通過日等三個不同時點的兩個超額報酬,相對於上市(櫃)審議委員會通過日至上市(櫃)掛牌日這段期間之超額報酬的影響。另解析個案公司SWOT分析結果,探討個案公司相對於其所面對外部環境之機會與威脅,以及公司內部環境所具備的優勢與劣勢等相關影響因素。 本研究運用複迴歸分析,得到實證結果發現興櫃申請上市(櫃)公司: 1.「登錄興櫃日至上市(櫃)送件日之超額報酬」主要與產(行)業別、興櫃交易天數、登錄興櫃日至上市(櫃)送件日之大盤漲幅、會計師事務所聲譽等因素有關。 2.「上市(櫃)送件日至審議委員會通過日之超額報酬」受到公司成立年數、興櫃交易天數、稅後EPS、登錄興櫃日至上市(櫃)送件日之大盤漲幅、上市(櫃)送件日至審議委員會通過日之大盤漲幅的影響。 3.「審議委員會通過日至上市(櫃)掛牌日收盤價之超額報酬」與產(行)業別、興櫃個股日平均成交金額、興櫃交易天數、中籤率、承銷價、登錄興櫃日至上市(櫃)送件日之大盤漲幅、上市(櫃)送件日至審議委員會通過日之大盤漲幅、審議委員會通過日至掛牌日收盤價之大盤漲幅、首五日無漲跌幅限制、會計師事務所聲譽有關。 4.「議定承銷價至上市(櫃)掛牌日收盤價之超額報酬」則是受到公司成立年數、興櫃交易天數、承銷價、稅後EPS、首五日無漲跌幅限制等變數的影響。 除上述影響因素外,個案分析興櫃申請上市櫃(IPOs)公司可發現若該公司在產業皆具有領導地位、產品當紅有競爭利基(如IC設計等)具有國際競爭力及成長潛力、掌握關鍵研發創新技術、營收或獲利不斷成長、有較高毛利率及稅後每股盈餘、部份有富爸爸資源支持及垂直整合從與外資青睞,從登錄興櫃經審議委員會到上市(櫃)掛牌皆有超額報酬現象。 / Emerging Stock Market, which was started the transaction on January 2, 2002, provides the investors a legitimate, safe and transparent stock transaction market of unlisted company. According to the listed examination criterion of TSEC and OTC, an initial public offerings (IPOs) company has to be listed and transacted on Emerging Stock Market for over than six months to be familiar with the transaction rule of market and disclose the information of the company fully transparently. Then it has the admissibility of applying to transfer to listed market. This thesis mainly explores the honeymoon effect of IPOs stock and its influence factors. The five purposes including: 1. To examine whether there is exceed return when an emerging company transferring to an IPOs company. 2. To discuss the influence to the exceed return of IPOs by the five group factors, including the company fundamental, the performance in Emerging Market, the conditions of the company at IPOs, the market situations and systems, and the company governance. 3. To discuss the influence to the exceed return of IPOs by the two time period factors between the three time points: listed day in the Emerging Market, the apply day to TSEC and OTC, and the day the application approved by Examination Committee of TSEC or OTC. 4. To give SWOT analysis (combined with five forces analysis) in the case studies to explore, of the target companies, the opportunities and threats of the external environment and the strengths and weakness of the internal environment. 5. To apply the exploration in Purpose 1, Purpose 2, and Purpose 3 to the target companies in the case studies. By using multiple regression model, we found the following results: 1. The exceed return from the listed day in Emerging Market to the apply day to TSEC or OTC is related to the industry, the days staying in Emerging Stock Market, the performance of TSEC index, and the reputations of accountant business offices. 2. The exceed return from the day applying to TSEC or OTC to the day the application approved by Examination Committee is related to the company age, the days a company staying in Emerging Stock Market, the EPS after tax, and the performance of TSEC index. 3. The exceed return from the day the application passed by Examination Committee to the closing price at listing day is related to the industry, the average amount of transaction in Emerging Stock Market, the days a company staying in Emerging Stock Market, the transaction lot size, the stock offering price, the performance of TSEC index, the 7% price limit has been cancelled under the new underwriting system in five trading days after IPO, the reputations of accountant business offices. 4. The exceed return from the IPOs price to the closing price at listing day is related to the company age, the days a company staying in Emerging Stock Market, the stock offering price, the EPS after tax, the 7% price limit has been cancelled under the new underwriting system in five trading days after IPO. Besides the above factors, we found, from the case studies, that IPO companies have the exceed return when they possess: the leadership in the industry, the international competitiveness and growth potential of their niche products, the key creative technology of R&D, the continuation growing of revenue and profit, the higher profit rate and the EPS after tax, the support of the parent group, and the foreign capital investors.
165

E-procurement adoption, its benefits and costs

Mahdillou, Hossein, Akbary, Jawid January 2014 (has links)
This thesis report aims at illustrating and describing how e-procurement can contribute in creating value and reducing overheads associated with the procurement process through adopting and implementing e-procurement systems and applications in the supply chain network of an organization. It is an obvious fact that e-procurement plays a vital role in managing the supply chain especially now that outsourcing has become very common in the business market environment around the world. The main purpose of our bachelor thesis work is to find out how e-procurement can contribute in creating value, reducing cost and gaining more profit out of the procurement process. Since this thesis report is meant to cover the available literature on procurement and e-procurement only limited areas of the supply chain management is presented.When it comes to methodologies, available literatures on procurement and e-procurement have been covered to extract the most plausible information and data related how e-procurement contributes in creating value and reducing costs and overheads in the supply chain management. First a framework of modern procurement processes and stages is given as a basis and then e-procurement is treated being the technologies and key factors in implementation of e-procurement.This thesis work tries to consolidate prevalent ideas as well as facts that have been tested by means of academic researches carried out by scholars of the field and interdisciplinary of industrial engineering. / Program: BSc in Industrial Engineering - International Business Engineering
166

Educação permanente em saúde: mecanismo indutor do processo educativo no cotidiano do trabalho

DIAS, Geyse Aline Rodrigues 29 February 2016 (has links)
Submitted by Edisangela Bastos (edisangela@ufpa.br) on 2017-04-12T11:47:47Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_EducacaoPermanenteSaude.pdf: 1540226 bytes, checksum: a17043c0b65fbc9b28721140fedb5bfe (MD5) / Approved for entry into archive by Edisangela Bastos (edisangela@ufpa.br) on 2017-04-12T11:48:22Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_EducacaoPermanenteSaude.pdf: 1540226 bytes, checksum: a17043c0b65fbc9b28721140fedb5bfe (MD5) / Made available in DSpace on 2017-04-12T11:48:23Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_EducacaoPermanenteSaude.pdf: 1540226 bytes, checksum: a17043c0b65fbc9b28721140fedb5bfe (MD5) Previous issue date: 2016-02-29 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / O estudo tem como objetivo desvelar o desenvolvimento da proposta de educação permanente em saúde (EPS) em um município paraense, identificando as dificuldades e a influência destas no processo educativo no cotidiano do trabalho em saúde, tendo em vista a necessidade de se discutir sobre a Política Nacional de Educação Permanente em Saúde (PNEPS), a fim de modificar as práticas, qualificar a assistência em saúde, avaliar suas influências e reflexos para consolidação do Sistema Único de Saúde (SUS). No referencial teórico, utilizam-se os pressupostos da concepção pedagógica libertadora de Paulo Freire. Participaram do estudo quarenta e três (43) sujeitos, entre coordenadores e demais profissionais da saúde envolvidos nas propostas de educação permanente do município de Benevides-PA. Trata-se de um Estudo de Caso único holístico, de abordagem qualitativa e, segundo seus objetivos, com uma estratégia explanatória e descritiva (YIN, 2005). O período de coleta de dados foi de fevereiro a junho de 2014, por meio de entrevista focada, orientada por Yin (2015), a partir da utilização de protocolo de entrevista. Utilizou-se para a análise uma estratégia analítica geral denominada “Contando com proposições teóricas”, orientada por Yin (2015), alicerçada nas proposições teóricas da Educação Permanente em Saúde e do Processo Educativo no Cotidiano do Trabalho em Saúde, seguida do procedimento analítico de Strauss; Corbin (2008), do qual emergiram no total três (03) categorias, quais sejam: “Compreensão sobre Educação Permanente em Saúde”, “Processo de Educação Permanente em Saúde vivenciado no Cotidiano do Trabalho”, “Importância da Educação Permanente no Cotidiano do Trabalho em Saúde”; e oito (08) subcategorias, quais sejam: “Conceito de Educação Permanente em Saúde”, “Objetivo da Educação Permanente em Saúde”, “Demandas das ações a serem desenvolvidas”, “Elaboração do Projeto de Educação Permanente em Saúde”; “Desenvolvimento das ações de Educação Permanente em Saúde”, “Avaliação das ações de Educação Permanente em Saúde”, “Dificuldades no desenvolvimento das ações de Educação Permanente em Saúde”, e “Influência da Educação Permanente em Saúde no cotidiano do trabalho”. Os resultados mostraram que o desenvolvimento da proposta de EPS implantada no município está em fase inicial, pois a partir do que orienta a PNEPS, é imprescindível a interação e articulação entre as esferas de gestão, equipes de saúde, o ensino e a comunidade, entretanto, evidenciou-se um processo de relação vertical, hierárquico e centralizador da gestão, com predomínio de processos educativos tradicionais, de repasse de informações com pouco ou nenhum processo de reflexão crítica da realidade. Compreende-se que o município vive uma fase desafiadora de implementação da EPS, nessa perspectiva, sugere-se priorizar e criar mais momentos e espaços coletivos, dialógicos de planejamento participativo com todos os envolvidos, para assim favorecer processos horizontais de produção de saúde, elaboração e desenvolvimento de atividades pedagógicas problematizadoras, crítico-reflexivas, que atendam as reais necessidades dos trabalhadores e usuários, bem como, contribuir à motivação e valorização profissional, desenvolvendo e ampliando os serviços e colaborando efetivamente com o processo de consolidação do SUS. / The study aims to reveal the development of health continuing education proposal (EPS) on a Pará municipality, identifying difficulties and their influence in the educational process in health work daily, in view of the need to discuss on the National Policy of Permanent Education in health (PNEPS) in order to modify the practices, qualify the health care, to evaluate their influences and reflexes to consolidation of the Unified health System (SUS). In the theoretical referential, are used the assumptions of liberating pedagogical concept of Paulo Freire. Participated in the study forty-three (43) subjects, including coordinators and other health professionals involved in permanent education proposals in the Benevides-PA municipality.It consists a study of holistic single case, from a qualitative approach and, according to its objectives, with an explanatory and descriptive strategy (YIN, 2005). The data collection period was from February to June 2014, through focused interview guided by Yin (2015), from the use of interview protocol. Was used to analyze a general analytical strategy named "Couting on theoretical propositions," directed by Yin (2015), founded on the theoretical propositions of Continuing Education in Health and Educational Process in the Labour Everyday Health, followed by analytical procedure Strauss; Corbin (2008), from which emerged a total of three (03) categories, which are : "Understanding on Permanent Education in Health", "Process of Continuing Health Education experienced in Labor Daily", "Importance of Continuing Education in Daily the work in Health "; eight (08) subcategories, namely: "Concept of Continuing Education in Health", "Objective of Continuing Education in Health", "Demands of actions to be taken", "Development of Permanent Education Project Health"; "Development of the actions of Continuing Education in Health", "Difficulties in the development of the actions of Continuing Health Education", "Evaluation of the actions of Continuing Education in Health" and "Influence of Continuing Education in Health in daily work." The results showed that the development of EPS proposal deployed in the city is in its initial phase, because from that guides the PNEPS, interaction and coordination is essential between the levels of government, health workers, education and the community, however , was evidenced of a vertical relationship process, management hierarchical and centralized, with a predominance of traditional educational processes, the transfer of information with little or no process of critical reflection of reality. It is understood that the municipality is experiencing a challenging phase implementation of EPS, in this perspective, it is suggested to prioritize and create more moments and collective spaces, and dialogic participatory planning with all stakeholders, so as to foster horizontal processes of health production, preparation and development of problem-solving educational activities, critical and reflective, that meet the real needs of workers and users, as well as contribute to the motivation and professional appreciation , developing and expanding the services and collaborating effectively with the SUS consolidation.
167

Independent component analysis of evoked potentials for the classification of psychiatric patients and normal controls / Ανάλυση ανεξάρτητων συνιστώσων προκλητών δυναμικών για ταξινόμηση ψυχιατρικών ασθενών και υγιών μαρτύρων

Κοψαύτης, Νικόλαος Ι. 18 February 2009 (has links)
The last twenty years presented increased interest for the study of cerebral processes caused by external events (stimuli). One of the most significant endogenous components of Evoked Potentials is the P600 component. The P600 component may be defined as the most positive peak in the time window between 500 and 800 msec after an eliciting stimulus. This component is thought to reflect the response selection stage of information processing. P600 component is usually less pronounced compared to other components, such as the N100 or the P300. Frequently the P600 component appears as a not-easily discernible secondary peak overlying the ascending negative-going slope of the P300 waveform. In our study we used ERP data from various groups of patients and healthy controls. Patients were recruited from the outpatient university clinic of Eginition Hospital of the University of Athens. The controls were recruited from hospital staff and local volunteer groups. The aim of the study is the implementation of classification systems for these groups, using P600 features. This is usually not achieved well using as features the ERPs amplitude and latency. So for that reason, in our study, we want to extract new features using advanced techniques for processing the original ERPs, such as the Independent Component Analysis (ICA) method. However as a precursor of ICA, is considered the Principal Component Analysis (PCA) method, which we used for comparison reasons to ICA. In the application of ICA we achieve the decomposition of the recorded signals in ICs, supposing temporally independent components and propose ICs selection techniques in order to recompose the P600 component. The next stage was the use of a classification method based on the features extracted using the original data, data extracted through PCA processing and ICA-processed data. First we applied Kolmogorov-Smirnov test to check the normality of the distribution of the features, then we used the Logistic Regression method for classification and finally we have done two implementations of classification using Probabilistic Neural Networks. The first implementation was done with the creation of 15 features from the P600 peak amplitudes from the subjects’ data and the second implementation was done with the creation of four meta-features from the subjects’ P600 amplitude data. The results show that the application of ICA, combined with the logistic regression classification technique, provides notable improvement, compared to the classification performance based on the original ERPs. The main merit of the application is that classification is based on single parameters, i.e. amplitude of the P600 component, or its latency or its termination latency, which are directly related to the brain mechanisms related to ERP generation and pathological processes. / Τα τελευταία 20 χρόνια παρουσιάζεται αυξημένο ενδιαφέρον για την μελέτη εγκεφαλικών επεξεργασιών που προκλήθηκαν από εξωτερικά γεγονότα (ερέθισμα). Ένα από τα πιο σημαντικά ενδογενή συστατικά των Προκλητών Δυναμικών είναι το συστατικό P600. Το συστατικό P600 μπορεί να οριστεί σαν η πιο θετική αιχμή στο χρονικό διάστημα μεταξύ 500 και 800 msec μετά από ένα εκλυτικό ερέθισμα. Το συστατικό αυτό θεωρείται ότι απεικονίζει το στάδιο επιλογής απόκρισης της επεξεργασίας πληροφορίας. Το συστατικό P600 είναι συνήθως λιγότερο έντονο συγκρίνοντας το με άλλα συστατικά, όπως το N100 ή το P300. Συχνά το συστατικό P600 εμφανίζεται ως μια δυσδιάκριτη δεύτερη αιχμή, επικαλύπτοντας την ανοδική αρνητική κλίση της κυματομορφής του P300. Στη μελέτη μας χρησιμοποιήσαμε δεδομένα ΠΔ από ποικίλες ομάδες ασθενών και υγιών μαρτύρων. Οι ασθενείς συλλέχθησαν από τη πανεπιστημιακή κλινική του Αιγηνήτειου Νοσοκομείου του Πανεπιστημίου Αθηνών. Οι υγιείς συλλέχθησαν από το προσωπικό του νοσοκομείου και ομάδες εθελοντών. Ο σκοπός της μελέτης είναι η εφαρμογή συστημάτων ταξινόμησης για αυτές τις ομάδες, χρησιμοποιώντας χαρακτηριστικά του P600. Αυτό συνήθως δεν επιτυγχάνεται καλά χρησιμοποιώντας σαν χαρακτηριστικά το πλάτος και τον λανθάνοντα χρόνο των ΠΔ. Για αυτό το λόγο, στην μελέτη μας, θέλουμε να εξάγουμε νέα χαρακτηριστικά χρησιμοποιώντας προηγμένες τεχνικές για επεξεργασία των αρχικών ΠΔ, όπως τη μέθοδο Ανάλυσης Ανεξαρτήτων Συνιστωσών (ICA). Εντούτοις ως πρόδρομο της ICA, θεωρείται η μέθοδος Ανάλυσης Κύριων Συνιστωσών (PCA), την οποία χρησιμοποιήσαμε για συγκριτικούς λόγους με την ICA. Στην εφαρμογή της ICA προχωρήσαμε στην αποσύνθεση των καταγραφόμενων σημάτων σε Ανεξάρτητες Συνιστώσες και διερευνήσαμε τρεις τεχνικές επιλογής ανεξαρτήτων συνιστωσών μέσω των οποίων επανασυνθέσαμε το συστατικό P600. Το επόμενο βήμα ήταν η χρήση μεθόδου ταξινόμησης βασισμένης στα χαρακτηριστικά που εξάχθηκαν χρησιμοποιώντας τα αρχικά δεδομένα, τα δεδομένα με επεξεργασία PCA και τα δεδομένα με επεξεργασία ICA. Πρώτα εφαρμόσαμε το τεστ Kolmogorov-Smirnov για τον έλεγχο της κανονικότητας της κατανομής των χαρακτηριστικών, μετά χρησιμοποιήσαμε τη μέθοδο Λογαριθμικής Παλινδρόμησης (Logistic Regression) για ταξινόμηση και τελικά πραγματοποιήσαμε δύο εφαρμογές ταξινόμησης χρησιμοποιώντας Πιθανοκρατικά Νευρωνικά Δίκτυα (Probabilistic Neural Networks). Η πρώτη εφαρμογή έγινε με την δημιουργία 15 χαρακτηριστικών από τα πλάτη των αιχμών του P600 από τα δεδομένα των ομάδων και η δεύτερη εφαρμογή έγινε με την δημιουργία τεσσάρων μετά-χαρακτηριστικών από τα δεδομένα των πλατών των ομάδων. Τα αποτελέσματα δείχνουν ότι η εφαρμογή της ICA, συνδυασμένη με την τεχνική ταξινόμησης λογαριθμικής παλινδρόμησης, παρέχει αξιοσημείωτη βελτίωση, συγκριτικά με την απόδοση ταξινόμησης βάση των αρχικών ΠΔ. Η κύρια αξία της εφαρμογής είναι ότι η ταξινόμηση πετυχαίνει ποσοστά μεγαλύτερα του 80% βασιζόμενη σε μία μόνο κάθε φορά παράμετρο, π.χ. το πλάτος του συστατικού P600, ή τον λανθάνοντα χρόνο του ή τον λανθάνοντα χρόνο τερματισμού του, οι οποίες σχετίζονται άμεσα με τους μηχανισμούς του εγκεφάλου σχετικούς με την παραγωγή ΠΔ και τις παθολογικές διαδικασίες.
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Stress response in the cyanobacterium Synechocystis sp. PCC 6803

Miranda, Helder January 2011 (has links)
Adaptation to environmental changes is important for the survival of living organisms. Under extreme abiotic conditions, organic molecules (such as lipids, proteins and nucleic acids) are prone to damage. Under these conditions stress response mechanisms are activated, either to prevent the source of damage or to promote the rapid turnover of damaged molecules. Like all photoautotrophic organisms, cyanobacteria are sensitive to high light intensity and oxidative stress, which induces damage to the photosynthetic apparatus. My thesis is divided in two subjects related to particular stress responses in the cyanobacterium Synechocystis sp. PCC 6803: 1) the role of Deg/HtrA proteases and 2) investigations on the small CAB-like proteins. Deg/HtrA proteases are ATP-independent serine endopeptidases with a characteristic C-terminal PDZ domain. These proteases are largely dispersed among living organisms, with many different functions, mostly involved in protein quality control. The genome of Synechocystis sp. PCC 6803 contains three genes coding for Deg/HtrA proteases: HtrA, HhoA and HhoB. These proteases are essential for survival under high light and heat stress, and may overlap in their functions. During my Ph.D. studies I focused on the identification of the precise localization of the Deg/HtrA proteases in the cyanobacterial cell, analyzed the biochemical properties of recombinant Synechocystis Deg/HtrA proteases in vitro and adopted proteomic and metabolomic approaches to study the physiological importance of these proteases. My data show that Deg/HtrA proteases are not only important in stress response mechanisms under adverse conditions, but are also involved in the stabilization of important physiological processes, such as polysaccharides biosynthesis and peptidoglycan turnover. The small CAB-like proteins (SCPs) belong to the light harvesting-like family of stress induced proteins and are thought to be involved in the photoprotection of the photosynthetic apparatus. Five small CAB-like proteins where identified in Synechocystis sp. PCC 6803 (ScpA-E). In my studies I identified another relative to the SCPs, LilA, which I found to be co-transcribed with ScpD. I also focused on the subcellular localization and identification of potential interaction partners of the SCPs.
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Caractérisation et modélisation des potentiels induits par les commutations des gradients de champ magnétique sur les signaux électrophysiologiques en IRM

El Tatar, Aziz 29 March 2013 (has links) (PDF)
Les développements récents des techniques IRM engendre des sources de potentiels induits qui " polluent " les signaux électrophysiologiques, utilisé simultanément en IRM pour surveiller le patient et synchroniser les images. Le système élaboré dans ce travail est compose de deux modules " émetteur-récepteur " IRM-compatibles. Le premier permet d'introduire dans le tunnel d'IRM des signaux EPS dont on connait les caractéristiques. Les signaux sont injectés dans un tissu conducteur placé dans le tunnel. Le second module permet de recueillir les signaux après leur contamination par les artéfacts générés par les séquences d'imagerie. Il comporte 20 canaux répartis en quatre bandes fréquentielles (40, 80, 160 et 350 Hz). Des mesures du potentiel induit ont été réalisées en environnements 1,5 T et 3 T. Nous pouvons ainsi analyser les modifications des paramètres des signaux selon les séquences, mais aussi à l'intérieur des différentes bandes de fréquences. Dans ce travail, nous présentons une caractérisation et modélisation des potentiels induits par les commutations de gradients de champ magnétique recueilli par notre dispositif expérimental. La caractérisation et la modélisation permettent d'obtenir des informations pertinentes à prendre en compte pour l'élaboration des algorithmes de filtrage efficaces et robustes.
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Can Companies Providing Public Sanitation Services Commercialize Treated Wastewater? / ¿Pueden las Empresas Prestadoras de Servicios Públicos Comercializar Agua Residual Tratada?

Monteza Palacios, Carlos, Monge Sayán, Fiorella, Aliaga Aliaga, Jenny 10 April 2018 (has links)
The authors of this article conducted an analysis of the companies providing publicsanitation services. In particular, they focus on determining if these are legally entitled to market the treated waste water generated as a result of the provision of public services in charge. Additionally, in order to provide a complete analysis of the issue, the authors speak about the powers of the National Water Authority and his power to permit reuse of treated wastewater. / Los autores del presente artículo realizan un análisis sobre las empresas prestadoras de servicios públicos de saneamiento. En particular, se enfocan en determinar si es que estas se encuentran facultadas legalmente para comercializar el agua residual tratada que generan como consecuencia de la prestación de los servicios públicos a su cargo. Adicionalmente, a fin de brindar un análisis completo del tema, se toman en cuenta las facultades de la Autoridad Nacional del Agua para conceder autorizaciones de reúso del agua residual tratada.

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