<p>Cadmium (Cd) is a highly mobile and toxic heavy metal that negatively affects plants, soil biota, animals and humans, even in very low concentrations. Currently, Cd contamination of cocoa produced in Latin American countries is a significant problem, as concentrations can exceed acceptable levels set by the European Union (0.5 mg/kg), sometimes by more than 10 times allowable levels. In South America, <i>Theobroma cacao</i> is an essential component of the basic market basket. This crop contributes to the Latin-American trade balance, as these countries export cacao and chocolate-based products to major consumer countries such as the United States and Europe. Some soil amendments can alter the bioavailability and uptake of Cd into edible plant tissues, though cacao plants can accumulate Cd without displaying any visible symptoms of phytotoxicity, which makes it difficult to determine if potential remediation strategies are successful. Currently, the only effective way to quantify Cd accumulation in plant tissues is via destructive post-harvest practices that are time-consuming and expensive. New hyperspectral imaging (HSI) technologies developed for use in high-throughput plant phenotyping are powerful tools for monitoring environmental stress and predicting the nutritional status in plants. Consequently, the experiments described in this thesis were conducted to determine if HSI technologies could be adapted for monitoring plant stress caused by Cd, and estimating its concentration in vegetative plant tissues. Two leafy green crops were used in these experiments, basil (<i>Ocimum basilicum L.</i> var. Genovese) and kale (<i>Brassica oleracea L</i>. var. Lacinato), because they are fast growing, and therefore, could serve as indicator crops on cacao farms. In addition, we expected these two leafy green crops would differ in their morphological responses to Cd stress. Specifically, we predicted that stress responses would be visible in basil, but not kale, which is known to be a hyperaccumulator. The plants were subject to four levels of soil Cd (0, 5, 10 and 15 ppm), and half of the pots were amended with biochar at a rate of 3% (v/v), as this amendment has previously been demonstrated to improve plant health and reduce Cd uptake. The experiments were conducted at Purdue’s new Controlled Environment Phenotyping Center (CEPF). The plants were imaged weekly and manual measurements of plant growth and development were collected at the same times, and concentrations of Cd as well as many other elements were determined after harvest. Fourteen vegetation indices generated using HSI images collected from the side and top view of plants were evaluated for their potential to identify subtle signs of plant stress due soil Cd and the biochar amendment. In addition, three mathematical models were evaluated for their potential to estimate Cd concentrations in the plant biomass and determine if they exceed safe standards (0.28 mg/kg) set by the Food and Agriculture Organization (FAO) for leafy greens. Results of these studies confirm that like many plants, these leafy green crops can accumulate Cd levels that are well above safety thresholds for human health, but exhibit few visible symptoms of stress. The normalized difference vegetation index (NDVI) and the chlorophyll index at the red edge (CI_RE) were the best indices for detecting Cd stress in these crops, and the plant senescence and reflectance index (PSRI) and anthocyanin reflectance index (ARI) were the best at detecting subtle changes in plant physiology due to the biochar amendment. The heavy metal stress index (HMSSI), developed exclusively for detecting heavy metal stress, was only able to detect Cd stress in basil when images were taken from the top view. Results of the mathematical models indicated that principal components analysis (PCA) and partial least squares (PLS) models overfit despite efforts to transform the data, indicating that they are not capable of predicting Cd concentrations in these crops at these levels. However, the artificial neural networks (ANN) was able to predict whether leafy greens had levels of Cd that were above or below critical thresholds suggested by the FAO, indicating that HSI could be further developed to predict Cd concentrations in plant tissues. Further research conducted in the field and in the presence of other environmental stress factors are needed to confirm the utility of these tools, and determine whether they can be adapted to monitor Cd uptake in cacao plants.</p>

No description available.

<p>Cereal rye (<i>Secale cereale</i>, L., CR) is the most commonly utilized cover crop species within the United States. Yet, the total land area planted to CR on an annual basis remains relatively low despite its numerous proven environmental benefits. The relatively low rates of CR adoption could be due to a dearth of knowledge surrounding certain agronomic and economic components of CR adoption. Currently, there exists knowledge gaps within the scientific literature regarding CR performance, N cycling, and associated economic risk. <a>Thus, to address the above-mentioned knowledge gaps, three individual studies were developed to: i) investigate the fate of scavenged CR nitrogen (N) amongst soil N pools, ii) assess the suitability of visible-spectrum vegetation indices (VIs) to predict CR biomass and nutrient accumulation (BiNA), and iii) characterize the economic risk of CR adoption at a regional scale over time.</a></p> <p>In the first study, ¹⁵N, a stable isotope of N, was used in an aerobic incubation to track the fate of CR root and shoot N among the soil microbial biomass, inorganic, and organic N pools, as well as explore CR N bioavailability over a simulated corn growing season. In this study, the C:N ratio of the shoot residues was 16:1 and the roots was 31:1 and differences in residue quality affected the dynamics of CR N release from each residue type. On average, 14% of whole plant CR N was recovered in the soil inorganic N pool at the final sample date. Correspondingly, at the final sampling date 53%, 33%, and less than 1% of whole plant CR N was recovered as soil organic N, undecomposed residue, and as microbial biomass N, respectively. Most CR N remained unavailable to plants during the first cash crop growing season subsequent to termination. This knowledge could support the advancement of N fertilizer management strategies for cropping systems containing cereal rye.</p> <p>In the second study, a commercially available unmanned aerial vehicle (UAV) outfitted with a standard RGB sensor was used to collect aerial imagery of growing CR from which visible-spectrum VIs were computed. Computed VIs were then coupled with weather and geographic data using linear multiple regression to produce prediction models for CR biomass, carbon (C), N, phosphorus (P), potassium (K), and sulfur (S). Five visible-spectrum VIs (Visible Atmospherically Resistant Index (VARI), Green Leaf Index (GLI), Modified Green Red Vegetation Index (MGRVI), Red Green Blue Vegetation Index (RGBVI), and Excess of Green (ExG)) were evaluated and the results determined that MGRVI was the best predictor for CR biomass, C, K, and S and that RGBVI was the best predictor for CR N and P. Furthermore, the final prediction models for the VIs selected as the best predictors developed in this study performed satisfactorily in the prediction of CR biomass, C, N, P, K, and S producing adjusted R² values of 0.79, 0.79, 0.75, 0.81, 0.81, and 0.78, respectively. The results of this study have the potential to aid producers in making informed decisions regarding CR and fertility management. </p> <p>In the final study, agronomic data for corn and soybean cropping systems with and without CR was collected from six states (Illinois, Indiana, Iowa, Minnesota, Missouri, and Wisconsin) and used within a Monte-Carlo stochastic simulation to characterize the economic risk of adopting CR at a regional scale over time. The results of this study indicate that average net returns to CR are always negative regardless of CR tenure primarily due to added costs and increased variability in cash crop grain yields associated with CR adoption. Further, the results demonstrate that the additional risk assumed by adopting CR is not adequately compensated for with current CR adoption incentive programs and that the risk premium necessary can be 1.7 to 15 times greater than existing incentive payments. Knowledge gained from this study could be used to reimagine current incentive programs to further promote adoption of CR.</p>

No description available.

The marine environment provides essential ecosystem services that are critical to the functioning of the earth’s life support system and the maintenance of human well-being. Marine Protected Areas (MPAs) are recognised as being the mechanism though which marine natural capital may be conserved. This thesis focuses on the value associated with marine conservation in a case study area, Lyme Bay, England where a ‘closed area’ was created in 2008. A review of literature spanning 20 years shows that despite sound ecological knowledge of a marine area, the reliance on traditional neo-classical economic valuations for marine spatial planning can obscure other issues pertinent to the ecosystem approach. A further valuation of the marine leisure and recreation industry shows that the industry is of economic significance and that the MPA enables the protection of the most valuable sites but has limited benefits for protecting the full resource base. In terms of ecological value, a ‘service orientated framework’ was developed to enable decision makers to understand the links between benthic species, ecological function and indirect ecosystem services. Results spatially identify which ecosystem services occur and demonstrate the value of the MPA in ensuring delivery of these ecosystem services. In relation to the social value of the MPA the research reveals that support for the MPA is strong amongst the majority of stakeholder groups. Values are expressed as the economic, environmental and social benefits of the MPA. However, there have been clear social costs of the MPA policy and these have been borne by mobile and static gear fishermen and charter boat operators. Each valuation methodology can inform decision making. Though, if ecosystem service valuation is to become a deliberative tool for marine conservation and planning, then there is a need for a larger societal discussion on what activities and trade-offs society considers acceptable.

No description available.

No description available.

We are currently faced with the global challenge of conserving biological diversity while also increasing food production to meet the demands of a growing human population. Land-use change, primarily resulting from conversion to production land, is currently the leading cause of biodiversity loss. This occurs through habitat loss, fragmentation of remaining natural habitats, and resulting edge effects. Land-sparing and land-sharing approaches have been discussed as alternative ways to engineer landscapes to mitigate biodiversity loss while meeting production objectives. However, these represent extremes on a continuum of real-world landscapes, and it will be important to understand the mechanisms by which adjacent land use affects natural remnant ecosystems in order to make local land-management decisions that achieve conservation, as well as production, objectives. This thesis investigates the impact of juxtaposing production and natural forest on the community-wide interactions between lepidopteran herbivores and their parasitoids, as mediated by parasitoid spillover between habitats. The first and overarching objective was to determine whether herbivore productivity drives asymmetrical spillover of predators and parasitoids, primarily from managed to natural habitats, and whether this spillover alters trophic interactions in the recipient habitat. The study of trophic interactions at a community level requires understanding of both direct and indirect interactions. However, community-level indirect interactions are generally difficult to predict and measure, and these have therefore remained understudied. Apparent competition is an indirect interaction mechanism thought to be very important in structuring host-parasitoid assemblages. However, this is known primarily from studies of single species pairs, and its community-wide impacts are less clear. Therefore, my second objective was to determine whether apparent competition could be predicted for all species pairs within an herbivore assemblage, based on a measure of parasitoid overlap. My third objective was to determine whether certain host or parasitoid species traits can predict the involvement of those species in apparent competition. My key findings were that there is a net spillover of generalist predators and parasitoids from plantation to native forest, and that for generalists, this depends on herbivore abundance in the plantation forest. Herbivore populations across the edge were linked by shared parasitoids in apparent competition. Consequently, an experimental reduction of herbivore density in the plantation forest changed parasitism rates in the natural forest, as predicted based on parasitoid overlap. Finally, several host and parasitoid traits were identified that can predict the degree to which host or parasitoid species will be involved in apparent competition, a finding which may have extensive application in biological control, as well as in predicting spillover edge effects. Overall, this work suggests that asymmetrical spillover between production and natural habitats occurs in relation to productivity differences, with greater movement of predators and parasitoids in the managed-to-natural forest direction. The degree to which this affected species interactions has implications for landscape design to achieve conservation objectives in production landscapes.

Increasingly unpredictable, extreme and erratic rainfall with higher temperatures threatens to undermine the adaptive capacity of food systems and ecological resilience of smallholder landscapes. Despite growing concern, land managers still lack quantitative techniques to collect empirical data about the potential impact of climatic variability and change. This thesis aims to assess how ecosystem services and function and how this links with biodiversity and human wellbeing in smallholder agro-ecosystems in a changing climate. To this end, rather than relying on scenarios or probabilistic modelling, space was used as a proxy for time to compare states in disparate climatic conditions. Furthermore, an integrated methodological framework to assess ecosystem services at the field and landscape level was developed and operationalised, the results of which can be modelled with measures of wellbeing. Various multidisciplinary analytical tools were utilised, including ecological and socio-economic surveys, biological assessments, participatory open enquiry, and documenting ethnobotanical knowledge. The study was located within monsoon rice farms in the Terai Plains of Nepal, and dry season vegetable farms in Northern Ghana. Sites were selected that are climatically and culturally diverse to enable comparative analysis, with application to broad areas of adaptive planning. The linkages that bring about biophysical and human changes are complex and operate through social, political, economic and demographic drivers, making attribution extremely challenging. Nevertheless, it was demonstrated that within hotter and drier conditions in Ghana long-tongued pollinators and granivores, important for decomposition processes and pollination services, are more abundant in farms. Results further indicated that in cooler and drier conditions in Nepal, the taxonomic diversity of indigenous and close relative plant species growing in and around farms, important for the provisioning of ecosystem services, decreases. All other things equal, in both Nepal and Ghana findings indicate that overall human wellbeing may be adversely effected in hotter conditions, with a potentially significantly lower yields, fewer months of the year in which food is available, higher exposure to natural hazards and crop loss, unemployment, and psychological anxiety. Yet, surveys indicate smallholders continue to maintain a fair diversity of species in and around farms, which may allow them to secure basic necessities from provisioning ecosystem services. Moreover, farmers may employ adaptive strategies such as pooling labour and food sharing more frequently, and may have greater access to communication, technology, and infrastructure. Novel methodological and empirical contributions of this research offer predictive insights that could inform innovations in climate-smart agricultural practice and planning.

The world-wide intensification of agriculture has led to a decline in species richness due to land use change, isolation, and fragmentation of natural and semi-natural habitats in agricultural and forestry landscapes. As a consequence, there is a current landscape management focus on the importance of green infrastructure to mitigate biodiversity decline and preserve ecosystem functions e.g. pollination services and pest control. Even though intensification in agriculture has been ongoing for several hundreds of years, remnant habitats from earlier management practices may still be remaining with a surprisingly high plant richness. Preserving these habitats could help conserving plant species richness in agricultural landscapes, as well as other organisms that are dependent on plants for food and shelter. In this thesis I focus on two small remnant habitats; midfield islets and borders between managed forest and crop field in southeastern Sweden. In the past, both habitats were included in the grazing system and therefore often still have remnant population of grassland specialist species left today. I have used these two remnant habitats as model habitats to investigate the effect of landscape factors and local factors on species richness of plants, flower morphologies and plants with fleshy fruits. Additively, I analysed the effect of surrounding landscape and local openness on the functions; pollination success, biological pest control of aphids and seed predation on midfield islets. One of my studies showed that spatial distribution and size of the habitat affected plant species richness. Larger habitat size and higher connectivity between habitats increased species richness of plants in the habitats. Openness of the habitats was shown to be an important factor to increase species richness and richness of flower morphologies, both on midfield islets and in forest borders. Even though midfield islets had the highest species and morphology richness, both habitat types are needed for habitat complementary as forest borders have more plants with fleshy fruits and a higher richness of plant species that flowers in spring/early summer. It was also shown that a more complex forest border, not just with gaps in the canopy, but also with high variation in tree stem sizes increases plant species richness in the field layer. The conclusion is that by managing small remnant habitats to remain or become more semi-open and complex in their structure, would increase species richness of plants, grassland specialist species, and flower morphologies. It would also increase some ecosystem functions as seed predation and biologic pest control of aphids are more effective close to trees. If both midfield islets and forest borders would be managed to be semi-open, the area and connectivity of semi-open habitat would increase in the agricultural landscape, which may also improve pollination success as the connectivity between populations has a possibility to increase. Grassland specialist species are clearly abundant in the small remnant habitats. As the decline of semi-natural grasslands is causing a decline in grassland specialists’ species, not only plants, I recommend that small remnant habitats are included in conservation and management plans and strategies to improve habitat availability and connectivity for grassland species in agricultural landscapes. / <p>Research funder Ekoklim. Project:4339602.</p><p>At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 4: Manuscript.</p>