Constructed wetland (CW) system is one natural purifying process. Using the CW systems to treat industrial wastewater or domestic sewage has been extensively application in many countries. Constructed wastewater treatment wetland must depend on the optimal operation parameters to achieve the best treatment efficiency. The objective of this research was to evaluate the effectiveness of Kaoping River Rail Bridge Constructed Wetland (KPCW) on contaminated river water treatment. The major influents came from the treated industrial wastewater from a paper mill [Yuen Foong Yu paper manufacturing company (System A)] and local drainage system (Chu Liao River) containing untreated domestic wastewater (System B).Results from this study show that the measured flow rates for Systems A and B systems were 10,968 and 13,147 m3/day, respectively. The hydraulic loading rates (HLR) and hydraulic retention time (HRT) for Systems A and B were 0.085 and 0.096 m/day, and 5.4 and 10.7 d, respectively. The average removal efficiencies for both systems ranged from 63.4-71.7% for biochemical oxygen demand (BOD), 39.5-44.4% for chemical oxygen demand (COD), 28.1-39.5% for ammonia nitrogen (NH4-N), 17.1-40.3% for total nitrogen (TN), 5.4-45.5% for total phosphorus (TP), and 91.1-98.7% for total coliform (TC). Reduction in suspend solid (SS) concentration was ineffective in both systems. This was due to the irregular harvest of the plants in the wetland. Results from the effluent probability method (EPM) evaluation indicate that the removal efficiency increased with the increase in influent pollutant concentration. Moreover, variations in pollutant loading rate (PLR) would affect both the removal rates and effluent concentrations. The experience obtained from this project will be helpful in designing similar natural water treatment systems for river water quality improvement for other river basins.

Produced water is formed in underground formations and brought up to the surface along with crude oil during production. It is by far the largest volume byproduct or waste stream. The most popular preference to deal with produced water is to re-inject it back into the formation. Produced water re-injection (PWRI) needs a treatment before injection to prevent formation blockage. Due to the increase of produced water during oil production in the west of Kuwait, an effluent treatment and water injection plants were established and commissioned in 2004 so that produced water could be used for re-injection purposes. It is estimated that oil wells in the west of Kuwait produce 15 to 40 % of produced water. The main aim of this treatment train is to reduce not only the oil-in-water amount to less than 10 ppm, but also total suspended solids to 5 ppm which is the maximum allowable concentration for re-injection and disposal. Furthermore, with respect to the upper limit for injection, the maximum number of particles between 5 and 8 microns is 200 in 0.1 ml. In practice the number is found to exceed this limit by 10 times...

On Site Inspection is the final measure for verifying compliance of Member States with the Comprehensive Nuclear-Test-Ban Treaty. In order to enable the use of ³⁷Ar as a radiotracer for On Site Inspection, the sources of radioargon background must be characterized and quantified. A radiation transport model of the University of Texas at Austin Nuclear Engineering Teaching Laboratory (NETL) TRIGA reactor was developed to simulate the neutron flux in various regions of the reactor. An activation and depletion code was written to calculate production of ³⁷Ar in the facility based on the results of the radiation transport model. Results showed ³⁷Ar production rates of (6.567±0.31)×10² Bq·kWh⁻¹ in the re- actor pool and the air-filled irradiation facilities, and (5.811±0.40)×10⁴ Bq·kWh⁻¹ in the biological shield. Although ⁴⁰Ca activation in the biological shield was found to dominate the total radioargon inventory, the contribution to the effluent release rate would be diminished by the immobility of Ar generated in the concrete matrix and the long diffusion path of mobile radioargon. Diffusion of radioargon out of the reactor pool was found to limit the release rate but would not significantly affect the integrated release activity. The integrated ³⁷Ar release for an 8 hour operation at 950 kW was calculated to be (1.05±0.8)×10⁷ Bq, with pool emissions continuing for days and biological shield emissions continuing for tens of days following the operation. Sensitivity analyses showed that estimates for the time-dependent concentrations of ³⁷Ar in the NETL TRIGA could be made with the calculated buildup coefficients or through analytical solution of the activation equations for only (n,[gamma]) reactions in stable argon or (n,[alpha]) reactions in ⁴⁰Ca. Analyses also indicated that, for a generalized system, the integrated thermal flux can be used to calculate the buildup due to air activation and the integrated fast flux can be used to calculate the buildup due to calcium activation. Based on the results of the NETL TRIGA, an estimate of the global research reactor source term for ³⁷Ar and an estimate of ground-level ³⁷Ar concentrations near a facility were produced. / text

Globally agricultural production is facing serious challenges to provide adequate food supply to meet a growing population. However, the reduced capacity of soil to support and sustain agricultural production as a result of soil fertility decline is impacting negatively on agricultural growth. Increase in the price of inorganic fertilisers and limited availability of nutrients from organic amendments has reduced progress in improving soil fertility. This research therefore aims at contributing knowledge towards evaluating the maximisation/optimisation of nutrients in compost and secondary treated sewage effluent (STSE) amended soils to meet the nutritional requirements of crops for sustainable crop production and environmental protection. STSE was irrigated on soils (sandy loam and clay loam) amended with greenwaste compost in soil incubation, glasshouse/pot and lysimeter studies. Perennial ryegrass (Lolium perenne) was grown in the pots and lysimeter studies. The incubation experiment showed that for a clay loam, N mineralisation in treatments with STSE alone and combinations of compost and STSE was higher than the applied N. Increasing compost quantity in compost and STSE nutrient integration resulted in reduced net N mineralisation in the clay loam soil. In the sandy loam, increasing compost contribution in compost and STSE nutrient integration resulted in an increase in net N mineralisation. Cation exchange capacity, microbial diversity, quality of available carbon and drying and rewetting cycles influenced the net nitrogen mineralisation dynamics in both soil types. Increasing the contribution of STSE while reducing compost quantity resulted in increased nitrogen use efficiency and ryegrass dry matter yield. The environmental threat to ground and surface water pollution through NO3 --N leaching may be enhanced by the inclusion of STSE in integrated compost and STSE nutrient supply to plants. Similarly, the threat to eutrophication due to phosphorous leaching is likely to be higher with integration of compost and STSE. Ryegrass dry matter yield reduced with increasing compost contribution while the concentration of N in ryegrass herbage for the combinations of compost and STSE was above the minimum requirement for N in herbage for productive grazing and dairy cattle in the pot experiment. Using compost and STSE of similar characteristics, the ideal approach to maximise nutrient potential from compost through irrigation with STSE is when 25% compost is integrated with 75% STSE with respect to nitrogen supply.

Pharmaceuticals and personal care products (PPCPs) are detected routinely in raw and treated municipal wastewater. Conventional wastewater treatment processes are not effective in removing PPCP; therefore, treated wastewater discharges are one of the main entry points for PPCPs into the aquatic environment, and eventually into drinking water supplies. The use of laccase-catalyzed oxidation for removing low concentrations of PPCPs from municipal wastewater after primary treatment is investigated. Oxybenzone was selected as a representative PPCP. Like many other PPCPs, oxybenzone is not recognized directly by the laccase enzyme. Therefore, mediators were used to expand the oxidative range of laccase, and the efficacy of this laccase-mediator system in primary effluent was evaluated. Eight potential mediators were investigated. The greatest oxybenzone removal efficiencies were observed when 2,2’-azino-bis(3-ethylbenzthiazoline-6sulphonic acid) (ABTS), a synthetic mediator, and acetosyringone (ACE), a natural mediator, were present. An environmentally relevant concentration of oxybenzone (10 µg/L) in primary effluent was removed below the detection limit after two hours of treatment with ABTS, and 95% was removed after two hours of treatment with ACE. Several mediator/oxybenzone molar ratios were evaluated at two different initial oxybenzone concentrations. Higher mediator/oxybenzone molar ratios were required at the lower (environmentally relevant) oxybenzone concentrations, and ACE required higher molar ratios than ABTS to achieve comparable oxybenzone removal. The oxidation mechanisms and kinetics of the ACE mediator was evaluated. A better understanding of the mediator oxidation process would lead to a better design of the laccase-mediator system. An alternative laccase-mediator treatment configuration, which allows the enzyme and mediator to react prior to coming in contact with the target PPCP, was investigated. This treatment configuration shows promise for further development since it might reduce laccase and mediator requirements. Oxidation byproducts generated by the laccase-mediator system were characterized and compared to those generated during ozonation. Enzymatic treatment generated byproducts with higher mass to charge (m/z) ratios, likely due to oxidative coupling reactions. The results of this study suggest that, with further development, a laccase-mediator system has the potential to extend the treatment range of laccase to PPCPs not directly recognized by the enzyme, even in a primary effluent matrix. / text

Competition over freshwater resources is increasing at local and global scales. Growing urban and suburban centers utilize surface and groundwater resources to meet municipal, industrial, and agricultural demands, often at the expense of riparian ecosystems. Paradoxically, those same urban centers produce a significant volume of treated effluent that can be reused to restore and sustain riparian systems. Use of effluent as a source of water for the environment raises important questions about the benefits and impacts of effluent on riparian functions and ecosystem services, particularly in the context of climate change and drought conditions. This dissertation addresses knowledge gaps surrounding riparian change and resilience along the effluent-dominated Upper Santa Cruz River in southern Arizona. Appendix A investigates whether the Netleaf hackberry (Celtis laevigata var. reticulata) tree can provide accurate information on historic changes in climatic and hydrological conditions. Results indicate that hackberry trees do record climate-related stress in annual ring-width patterns and can therefore provide a historic frame of reference against which to compare current and future changes in riparian conditions. Appendix B documents spatial and temporal patterns of effluent uptake by Fremont cottonwood trees (Populus fremontii) through development of a new application for dendrochronology, specifically dendrochemistry. Results show that annual tree rings contain temporally variable concentrations of a micropollutant found only in effluent and may have the potential to record spatial and temporal patterns of effluent dispersion in riparian ecosystems. Appendix C investigates the complex interactions of ecohydrological conditions that led to a riparian mortality event along the Upper Santa Cruz River in 2005. Effluent is shown to contribute to riparian vegetation expansion, but also, due to its consistent delivery of nutrients and water, homogenize the system and ultimately diminish its resilience to perturbations and stress. Results highlight the paradoxical nature of effluent as both a contributor to riparian growth and a potential impediment to riparian function. This paradox can be resolved through a well-defined effluent impact monitoring and assessment program that incorporates historic information as well as current trends to detect significant changes in ecosystem functions and services.

The objectives of this project are to rate three approaches for deriving effluent requirements; create a prioritized list of improvement steps; apply a watershed-level model to determine treatment plant requirements; and provide recommendations for deriving effluent requirements in Ontario. Results of a two-part stakeholder survey show that until advancements are made, compromise is necessary when selecting an approach for deriving effluent requirements, as no one approach meets all the evaluation criteria. However, the necessary steps toward improvement are relatively clear and require multi-disciplinary input. When the watershed-level modelling approach was applied, it was found that although there are challenges that must be addressed, overall, it appears advantageous to use a tool such as watershed-level models for the purpose of deriving effluent requirements in Ontario. / Natural Sciences and Engineering Research Council of Canada, Ontario Graduate Scholarship Program

The removal of colour from effluent after dyeing of cellulosic fibres is a major problem due to the difficulty in treating such effluents by conventional treatment methods. Cellulosic fibres of a specific shade (colour) are produced by reacting the fibres with a mixture of reactive dyestuffs, salt and auxiliary chemicals. Reactive dye exhaustion to the fibre is about 80% i.e. 20% remains in the effluent; this is hydrolysed dye which cannot be reused for dyeing. The exhausted reactive dye bath together with first rinse represents 6 to 30 L effluent/kg of fabric and most of the colour and salt. The total water consumption for reactive dyeing ranges from 25 to 100 L effluent/kg fabric while the water consumption from total textile finishing ranges from 30 to 150 L effluent/kg fabric. Thus the concentration of the dye and salts are 5 times more concentrated when obtained at source. The use of activated carbon adsorption for the removal of colour from exhausted dyebath for the re-use of water, salt and energy has been studied. Four different commercial reactive dye chemistries and associated auxiliaries were tested and commercial activated carbon was used as an adsorbent. Different temperatures (20°C to 100°C), pH values (acidic, neutral, basic) and salt concentrations (50 g/L to 100 g/L) were evaluated and the high adsorption results were achieved when using high temperature, low pH (acidic) and high salt concentrations. The Freundlich adsorption parameters were obtained for activated carbon adsorption capacity and adsorption bond strength between reactive dyes and activated carbon. The overall removal of the reactive dye shades was 36% to 53% (based on colour in the effluent), salt recovery was about 94% to 97%, the water recovery ranged from 14% to 25% and energy savings of 17% to 32% could be achieved. Column adsorption test were performed at different temperatures and flowrates. The saturated activated carbon was regenerated and repeatedly used by elution with 1.0 M NaOH. The regenerated carbon was found to be effective up to 3 cycles of operations. A column system for treatment of reactive dyes using activated carbon was designed. The outcome of this study showed that treatment of reactive dyebath with activated carbon adsorption technique can allow re-use of water, salt and energy, thus enabling environmental improvements with a savings in salt, energy, water and treatment costs. / Thesis (M.Sc.Eng.)-University of KwaZulu-Natal, Durban, 2007.

The impact of septic tank effluent disposal on groundwater quality was investigated at Oxford. The Oxford township can be regarded as typical of many small communities on the Canterbury Plains which have a high density of septic systems serviced by soakage pit drainage. The primary concern with grouped septic systems is the potential for both chemical and microbial groundwater contamination. The alluvial gravel aquifers of the Canterbury Plains are especially susceptible to microbial contamination due to the high rates of groundwater flow which may transport both bacteria and viral contaminants over large distances. Geological investigations established the presence of an areally extensive, tuff derived, clay unit which forms an aquitard beneath the unconfined aquifer in the north of the Oxford area. Recharge of the unconfined aquifer above the clay unit is exclusively from rainfall infiltration while to the south, groundwater levels respond to rainfall infiltration and influent seepage from the Eyre River. The presence of two hydrogeologically distinct gravel units within the unconfined aquifer was determined by application of the column dilution technique. Point dilution tests showed the average groundwater velocity of 130 m/day in the upper gravel unit to be significantly higher than the 40 m/day measured in the underlying gravels. The presence of discrete channels of preferred flow within the unconfined aquifer system was also established by point dilution tests, intrachannel velocities ranging from 210 to 400 m/day. A resistivity salt tracing test indicated groundwater flow in an easterly direction with a velocity of 250 m/day through an observed channel feature. Groundwater quality monitoring showed a significant degree of groundwater contamination close to the Oxford township. Concentrations of faecal coliform bacteria in excess of drinking water standards were detected up to 900 m downgradient of the nearest septic tank. Elevated levels of chemical indicators (N03-N, CL-) were also detected in all monitoring wells. The pattern of groundwater contamination was complex, reflecting both the heterogeneity of groundwater flow through the unconfined aquifer system and the influence of monitoring well location. Predictive modelling indicated the potential for the transport of faecal coliform bacteria up to 2.6 km downgradient of Oxford. Modelling also suggested increased urban development within Oxford to have a relatively minor effect on the overall extent of groundwater contamination. Additional hydrogeological and water quality data, aided by the application of numerical solute transport modelling techniques, may provide a more accurate estimate of the impact of septic tank effluent disposal on groundwater quality. Future sewage disposal options for Oxford have to balance the low potential for microbial contamination of drinking water supplies outside the groundwater zone delineated by this study, against the environmental acceptability continuing contamination of this zone. This study has identified the need for further research into the effects of septic tank effluent discharge on groundwater quality in the Canterbury region, to provide a sound base for future resource management decisions.

Municipal wastewater effluent (MWWE) is an important source of pollution in the aquatic environment impacting fish. MWWE is a complex mixture of chemicals including pharmaceuticals, personal care products, industrial chemicals and pesticides. A link between reproductive endocrine disruption and MWWE exposure has been established in fish, but less is known about the effects of MWWE on non-reproductive endocrine disruption. The overall objective of this thesis was to examine the impacts of MWWE exposure on the stress response and intermediary metabolism in rainbow trout (Oncorhynchus mykiss). In fish, the primary adaptive organismal stress response involves the activation of hypothalamic-sympathetic-chromaffin axis to produce catecholamines, predominantly epinephrine, and the hypothalamic-pituitary-interrenal (HPI) axis to produce cortisol. Both of these hormones play a key role in elevating plasma glucose levels that is essential to fuel the increased energy demand associated with stress. Along with the organismal stress response, the cellular stress response, involving the synthesis of a suite of heat shock proteins (hsps), also plays an important role in protecting cellular protein homeostasis in response to stressors, including toxicants. The impact of MWWE on stress-related pathways were identified using a low-density trout cDNA microarray enriched with genes encoding for proteins involved in endocrine-, stress- and metabolism-related processes. This was further confirmed by assessing plasma hormone and metabolite levels and stress-related targeted genes and proteins expression and enzyme activities in select tissues in rainbow trout. Studies were carried out in controlled field (caging) and laboratory experiments to examine the impacts of MWWE on stress and tissue-specific metabolic responses in rainbow trout. Further in vitro studies using rainbow trout hepatocytes in primary cultures were carried out to investigate the mechanism of action of two pharmaceuticals, atenolol and venlafaxine, found in relatively high concentrations in MWWE in impacting the stress-mediated glucose response. In caged fish, MWWE exposure significantly elevated plasma cortisol and glucose concentrations, and altered the mRNA abundance of a number of stress-related genes, hormone receptors, glucose transporter 2 and genes related to immune function. When fish were exposed to an acute handling stress following a 14 d exposure to MWWE, the cortisol response was abolished and the glucose response was attenuated. The effects on cortisol did not correlate with changes in the expression of genes involved in cortisol biosynthesis, but were associated with an increase in hepatic glucocorticoid receptor (GR) protein expression. Upon further investigation in controlled laboratory studies, MWWE exposure elevated constitutive hsp 70 and hsp90 expression after 8 d exposure, which correlated with a decrease in glycogen levels in the liver in fish exposed to a high concentration of MWWE compared to control fish, pointing to a MWWE-induced increase in liver energy demand. By 14 d, glycogen stores were replenished, and this was commensurate with increases in liver gluconeogenic capacity, including increases in the activities of phosphoenolpyruvate carboxykinase (PEPCK) and alanine aminotransferase (AlaAT), along with a decrease in liver GR expression. In the heart, GR protein expression increased in treated fish, and the activity of pyruvate kinase increased, indicating an increase in glycolytic capacity. Subjecting the MWWE exposed fish to a secondary handling disturbance (acute stress) led to an attenuated plasma cortisol and glucose response compared to the control group. This corresponded with a reduced liver gluconeogenic capacity and a lower liver and heart glycolytic capacities, reflecting a disturbance in the energy substrate repartitioning that is essential to cope with stress. While it is difficult to establish causative agents from a complex mixture such as MWWE, the two pharmaceutical that were tested impacted glucose production. Specifically, atenolol and venlafaxine disrupted the epinephrine-induced glucose production, but did not modify cortisol-mediated glucose production in trout hepatocytes. The suppression of epinephrine-mediated glucose production by atenolol and venlafaxine was abolished by cAMP analogue (8-bromo cAMP) or glucagon (a metabolic hormone that increases glucose production). This suggests that both drugs disrupt β-adrenoceptor signaling, while it remains to be determined if the response is receptor isoform-specific. Altogether MWWE exposure disrupts the organismal and cellular stress responses in trout. Key targets for MWWE impact leading to the impaired cortisol and metabolic responses to stress include liver and heart GR expression, liver gluconeogenic capacity, and liver, heart and gill glycolytic capacities. Most significantly, MWWE impairs the ability to metabolically adjust to a secondary acute stressor, which is an important adaptive process that is integral to successful stress performance. From an environmental stand-point, long-term exposure to MWWE will lead to reduced fitness and will compromise the capacity of fish to cope with additional stressor, including escape from predators.