House flies (Musca domestica L.) are common pests affecting horses and their owners. Control of house flies in stable yards is currently based on the use of pesticides. However, the development of resistance by these flies to most pesticide groups has motivated horse owners to seek alternative methods of fly control. An entomopathogenic fungus, Beauveria bassiana (Bb) and an entomopathogenic bacterium, Bacillus thuringiensis var. israelensis (Bti) are two biological agents known to have activity against house flies. The broad objective of this study was to evaluate the effect of these two biological control agents on house flies in an equine environment. Using a structured questionnaire, presented in Chapter 2, thirty horse owners in KwaZulu-Natal were asked about the nuisance value of house flies, their current control measures, the potential market for biocontrol agents against house flies, and each owner’s perception of biocontrol methods. The horse owners were using three methods of house fly control namely, physical, chemical and biological. Most horse owners (97%) wanted access to effective biocontrol agents for control of house flies. Most horse owners (80%) stabled their horses at night, some or all of the time. The resultant manure piles in the stable yard were considered to be the primary cause of house fly problems. About 64% of the horse owners were dissatisfied with the currently available methods of controlling house flies in this situation. Chapter 3 covers two observational trials in which varying doses of Bacillus thuringiensis var. israelensis (Bti) were fed to horses, in order to identify a baseline dosage to give to horses in order to adequately control house fly populations growing in horse manure. The bacterium Bti, grown on wheat bran, was fed to six miniature horses at doses of 0, 0.125, 0.25, 0.5, 0.75, and 1.0 g per meal in Trial 3a, and at 0, 0.5, 1, 2, 4 and 8 g per meal in Trial 3b. Faeces were collected three times a week for 11 weeks and placed in incubation trays to allow the number of emerging adult house flies and closed pupae to be counted. In Trial 3a, there was a significant reduction in the number of closed pupae with an increase in Bti in the feed. The regression equation suggests that there will be 3.1 times as many closed pupae in the faeces when horses are fed 1 g of Bti in their feed, than when horses are fed no Bti. This dosage is the minimum baseline dosage for future trials. v Using manure from horses dosed in Trials 3a and 3b, the survival of the bacterium through the gut of horses was evaluated using a standard isolation technique. The growth of Bt colonies on the manure after the Bt isolation technique showed that some of the bacterial cells survived transition through the digestive tract of the horse. This study was qualitative in nature and did not attempt to quantify the level of Bti spore survival. These two observations suggest that Bacillus thuringiensis var. israelensis has the potential to be used as a biocontrol agent, applied via horse feed, for the control of house flies in stable yards. Future clinical trials, with appropriate replication, should be conducted using 1 g Bti/meal as the lowest test dosage. The objective of Chapter 4 was to determine whether spraying Bti or Bb on to horse manure is effective in the control of house flies. Over a six week period, two spraying trials were conducted in which increasing doses of Bb and Bti were sprayed on to 500 g samples of horse manure. Counts of house fly pupae and adults were taken. The doses of Bb and Bti tested were 0, 1, 2, 4 g in Trial 4a, and 0, 4, 8 and 12 g in Trial 4b. The research reported in Chapter 4 was characterized by the unexpectedly high levels of biological variation in egg, larvae and pupae numbers that were found in samples of horse manure, taken from the same skip two days apart. The statistical design of the two trials conducted was inadequate to cope with the high level of variation about treatment means for fly and larval counts. However, despite the lack of significant differences between treatment means, there is observational evidence that suggests that both Bb and Bti do have an effect on house fly survival. A simplified statistical model, which compared the number of hatched house flies on untreated manure, with the number on manure treated with any level of Bb (1 to 4 g /250 ml water), found a significant reduction in the number of hatched flies on treated manure. There was no significant corresponding reduction in the number of closed pupae, which suggests that Bb acts primarily before the larva pupates. The optimal dose of Bb and Bti to be sprayed on to manure could not be determined because of the high variation about treatment means. It is suggested that, in future trials similar dosages for Bb could be tested, but that higher dosages of Bti (starting at 2 g/250 ml water) should be used. Trial periods should be extended and replication increased dramatically to reduce variation about treatment means. Transformation of data before analysis may also be necessary to equalize variation about treatment means. / M.Sc.Agric. University of KwaZulu-Natal, Pietermaritzburg 2013.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Document formatted into pages; contains xv, 126 p.; also includes graphics (some col.). Includes bibliographical references (p. 96-107). Available online via OhioLINK's ETD Center

Made available in DSpace on 2014-06-11T19:32:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-25Bitstream added on 2014-06-13T18:44:28Z : No. of bitstreams: 1 garcia_mv_dr_jabo.pdf: 282112 bytes, checksum: 05e978f0649d572c4c498e6b838390fe (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Este trabalho objetivou avaliar o efeito da aplicação de Metarhizium anisopliae em pastagem de Brachiaria decumbens naturalmente infestada, submetida ao pastejo com bovinos, visando o controle do carrapato Boophilus microplus. O estudo foi realizado no período de maio de 2006 a março de 2007, em pastagem de B. decumbens, implantada em solo de textura argilosa da Fazenda do Glória, pertencente à Universidade Federal de Uberlândia (UFU), em Uberlândia, MG. Utilizou-se população de B. microplus naturalmente existente na pastagem com área de 12.000 m2 e 24 novilhos mestiços com cerca de 1 ano de idade, como hospedeiros experimentais. A randomização dos animais foi feita pela contagem de fêmeas ingurgitadas (instar entre 4,5 a 8 mm de comprimento), no terceiro, segundo e primeiro dias anteriores à primeira aplicação do fungo na pastagem. A área da pastagem foi então dividida em dois pastos com 6.000 m2, e grupos homogêneos de 12 animais foram introduzidos em cada pasto, determinando-se por sorteio o pasto tratado e o controle. Para pulverizar o pasto tratado, foram utilizados 5 a 6 kg de arroz contendo o isolado E9 de M. anisopliae esporulado, com os quais se produziu 400 L de suspensão conidial, quantidade suficiente para pulverizar, em média, 66,7 mL de suspensão por metro quadrado de pasto... / In this work the efficacy of spraying Brachiaria decumbens pasture with Metarhizium anisopliae against natural infestation with the cattle tick Boophilus microplus was evaluated. The study was conducted from May 2006 to March 2007 at the Gloria farm from the Federal University of Uberlândia, in Uberlândia, Minas Gerais, Brazil. Experimental pasture of 12.000 m2, naturally infested with B. microplus and had 24 one year old mixed breed bovines was used. The area was halved in two pastures of 6.000 m2 and each pasture received 12 animals. Tick susceptibility of bovines of each area was similar. Pastures were than sprayed with either a conidial suspension of E9 M. anisopliae isolate (treated pasture) or the conidial suspension vehicle without the fungus (control pasture). Pastures were sprayed 12 times with 21 day intervals between applications. Bovines remained on the pasture during the spraying. Fungus persistence on the soil and the grass was evaluated on first, seven and 14 days after each spraying. Pasture infestation with larvae and animal infestation with engorging female ticks were evaluated 17, 19, 21, 26 and 33 days after each pasture treatment ...(Complete abstract, click electronic access below)

Made available in DSpace on 2014-06-11T19:32:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-10-30Bitstream added on 2014-06-13T21:05:10Z : No. of bitstreams: 1 prette_n_dr_jabo.pdf: 397603 bytes, checksum: f2f29adeabc306b7b382c01f4d6b99d5 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O presente trabalho teve como objetivo avaliar “in vitro”, a ação de Beauveria bassiana e Metarhizium anisopliae, para larvas, ninfas e adultos machos e fêmeas não alimentadas de Rhipicephalus sanguineus e para onde as mesmas fases do ciclo de vida do ácaro mantidas alimentadas em cães por meio de câmaras de alimentação coladas no dorso do animal. A ação no ambiente habitado pelo cão foi avaliada por meio de infestações naturais em boxes com todos os estádios do ácaro. Foram usadas suspensões contendo 108 conídios mL-1 de cada isolado. Nos ensaios com as fases não alimentadas os estádios foram imersos nas suspensões dos fungos e no ensaio com de alimentação no cão os diversos estádios do ciclo biológico do carrapato foram imersos ou pulverizados com as suspensões de conídios, e no ensaio do ambiente do cão as suspensões foram pulverizadas em todo o canil. Nos ensaios “in vitro” foram avaliadas a mortalidade de larvas, ninfas e adultos machos e fêmeas, e no ensaio com alimentação no cão avaliou-se a mortalidade de cada estádio e os indivíduos sobreviventes foram colocados para ingurgitar em coelhos, para avaliação dos estádios subseqüentes do ciclo do carrapato, até se obter novamente a mesma fase em que iniciaram o ciclo. No ensaio do ambiente do cão foi avaliada diariamente a infestação dos animais com as diversas fases do ciclo de vida do ácaro. B. bassiana e M. anisopliae promoveram significativa redução da sobrevivência de todos os estádios não alimentados, deixando claro a atividade patogênica para a fase não alimentada de R. sanguineus. No experimento com a alimentação em cães, B. bassiana promoveu mortalidade de fêmea, mas não houve mortalidade de larvas e ninfas, fato este também observado para M. anisopliae... / This work aimed to assess the in vitro” action of Beauveria bassiana and Metarhizium anisopliae, for unfed larvae, nymphs and adult male and female of Rhipicephalus sanguineus and for the same stadiums the tick life cycle maintained fed on dogs through feeding cameras fixed on dog’s dorsal region. The action on the ambient where the dog lives was also evaluated through natural infestations with all of the stadiums of the acarid. Suspensions containing 108 conidia mL-1 of each isolated were used. In every assay with the unfed phase, the stadiums were immersed in the suspensions of the fungus and in the assays with feeding cameras, all the phases of the biological cycle of the tick were immersed or powdered with the conidial suspension and in the assay in the ambient suspensions were spread powdered in the whole box. In the in vitro assays it was evaluated the larvae, nymphs and adult male and female mortality. In the feeding cameras assay, the mortality of each stadium was evaluated and the surviving individuals were put for engorged in rabbits, to evaluate subsequent stadiums of the cycle of the tick, until obtain the same stadium wich was attained at the moment they began the cycle. In the environmental assay it was daily evaluated the infestation of the animals with all the phases of the life cycle of the acarid. B. bassiana and M. anisopliae promoted a significant reduction of the survival rate of all unfed stadiums of the tick what brings a strong evidence of pathogenicicity to unfed phases of R. sanguineus. In the experiment with feeding cameras, B. bassiana promoted female mortality, but mortality of larvae and nymphs were not observed fact this also observed for M. anisopliae. In the environment of the dog naturally infested with the stadiums of R. sanguineus was possible to observe that B. bassiana reduced the number of unfed and engorged larvae in the first week after treatment ...(Complete abstract click electronic access below)

Orientador: Sérgio Antonio de Bortoli / Banca: Nelson Wanderley Perioto / Banca: Luis Garrigós Leite / Banca: Ricardo Antonio Polanczyk / Banca: Alexandre de Sene Pinto / Resumo: O objetivo deste trabalho foi avaliar a ação do produto comercial Agree® nas características biológicas de três inimigos naturais em laboratório, utilizando a traça-das-crucíferas, P. xylostella. Os experimentos foram conduzidos em sala climatizada a 25±1°C, umidade relativa de 70±10% e fotofase de 12 horas. Foram verificados os aspectos biológicos de P. nigrispinus, Orius insidiosus e Trichogramma pretiosum por meio da ação do produto. Para os predadores foram elaboradas tabelas de vida de fertilidade. Os dados biológicos obtidos indicaram que quando P. nigrispinus se alimenta de lagartas infectadas com o produto os predadores sofrem efeito negativo em sua biologia, provocado provavelmente por alterações fisiológicas na lagarta. No experimento com O. insidiosus as características duração do segundo instar ninfal, o consumo ninfal e a longevidade das fêmeas foram afetadas pela ação do produto. Para T. pretiosum o produto não apresentou efeitos negativos que possam inviabilizar sua utilização conjunta com o produto para o Manejo Integrado de P. xylostella em brassicáceas. Apesar de o produto ter provocado efeitos negativos a P. nigrispinus e O. insidiosus estudos e técnicas complementares devem ser realizados para que, no futuro, esses inimigos naturais possam ou não ser utilizados em programas de controle biológico em culturas de brassicáceas associados com a bactéria entomopatogênica / Abstract: This work aimed to evaluate the action of biological insecticide Agree on biological characteristics of 3 natural enemies in laboratory, using the host diamondback moth, Plutella xylostella. The experiments were conducted in acclimatized room under 25±1°C, 70±10% relative umidity and 12 h photophase. The biological aspects of Podisus nigrispinus, Orius insidiosus and Trichogramma pretiosum were verified through the product action. Life and fertility table were elaborated to the predators. The biological data obtained showed that P. nigrispinus has the biology affected negatively when fed on infected caterpillars, probably occurred by physiological alterations in caterpillar. The characteristics duration and consumption of second nymphal instar, nymphal consumption and female longevity were affected by products in the assay with Orius insidiosus. The insecticide didn't show negative effects that can be unfeasible the use associated with the parasitoid T. pretiosum in the Integrated Pest Management to P. xylostella in brassicas. Thus the product cause negative effects on P. nigrispinus and O. insidiosus, complementary studies need to be realized in order to the future, these natural enemies can be used in Biological Programs associated with Bt in brassicas crops / Doutor

This thesis is focused on evaluation of the compatibility of entomopathogenic fungus Isaria fumosorosea with different species of entomopathogenic fungi and mycoparasitic fungi at different temperatures. The strains of species Beauveria bassiana, Metarhizium anisopliae, Lecanicillium muscarium, Coniothyrium minitans and Clonostachys rosea f. catenulata were selected for experiments base on compatibility. The results showed that combination of I. fumosorosea with species L. muscarium is compatible. The species do not limit to each other in the environment at all temperatures (15, 23 and 25 °C). On the other side, fungus I. fumosorosea in combination with other species have affected their growth and spore production. The efficacy of entomopathogenic fungi against larvae Tenebrio molitor was evaluated. The most effective species against larvae were species I. fumosorosea, B. bassiana and M. anisopliae. On the contrary, the smallest effective was observed after infection larvae by L. muscarium. Mycoparasitic fungus C. rosea f. catenulata was not able to directly infect larvae of T. molitor. This species did not infect healthy larvae. However it is able to infect weakened individuals or is growing as saprotrophs on the cadavers.

Diploma Thesis concerns with usage of entomopathogenic fungi in system of biological control against greenhouse whitefly Trialeurodoes vaporariorum and sweetpotato whitefly Bemisia tabaci. The Aim of diploma thesis was through in vivo and in vitro experiments determinated the basic evolutional and vegetative characteristics of selected entomopathogenic fungi and compare their effectivity in completed system {\clqq}plant - pest - pathogenic``. All experiments and results are demonstrated in the tables and the graphs. The photo-documentation of the method used during founding and interpretation of experiments is the part of the thesis.

Made available in DSpace on 2014-06-11T19:26:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-12-03Bitstream added on 2014-06-13T20:14:46Z : No. of bitstreams: 1 marucci_sc_me_jabo_parcial.pdf: 42548 bytes, checksum: 705ccf6a74fac7e11f4cb25dc8fb9403 (MD5) Bitstreams deleted on 2015-01-16T10:37:51Z: marucci_sc_me_jabo_parcial.pdf,Bitstream added on 2015-01-16T10:38:35Z : No. of bitstreams: 1 000638549.pdf: 1096070 bytes, checksum: 93634385c53868612602ba315f79ea43 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Como uma alternativa para diminuir as agressões constantes que o ecossistema vem sofrendo, devido à grande quantidade de produtos químicos utilizados no controle de pragas, pesquisas envolvendo microrganismos capazes de promover o controle biológico tem se intensificado. Dentre estes microrganismos a bactéria Bacillus thuringiensis tem se destacado. Essa bactéria caracteriza-se pela produção de proteínas tóxicas a representantes de diversas ordens de insetos. Em particular, as proteínas Vip3A, estão em amplo estudo devido a sua especificidade, alto potencial ativo e como alternativa para o controle da resistência de insetos às proteínas Cry. Diante disto, o objetivo deste trabalho foi selecionar, a partir de 1080 isolados de diferentes regiões brasileiras, aqueles portadores de genes vip3A e obter a sequência de nucleotídeos completa dos mesmos. As linhagens padrão B. thuringiensis var. kurstaki HD1, B. thuringiensis var. tolworthi HD125 e o isolado I187 tiveram seus DNAs amplificados com oligonucleotídeos baseados na sequência de genes vip3A, descritos no banco de dados de B. thuringiensis e, a partir dos amplicons obtidos, a sequência completa de nucleotídeos dos mesmos foi determinada, utilizando-se da estratégia de “primer walking”. A proteína Vip3Aa43 (GenBank: [HQ594534]) da linhagem HD1 demonstrou ser 100% idêntica às proteínas Vip3Aa já descritas. Já as proteínas Vip3Aa42 (GenBank: [HQ587048]) da linhagem HD125 e Vip3Ag5 (GenBank: [HQ542193]) do isolado I187 demonstraram similaridade de 99% com as proteínas descritas Vip3Aa35 e Vip3Ag2, respectivamente, demonstrando serem duas novas proteínas Vip3A, devido às substituições de aminoácidos ocorridas. Os três genes vip3A obtidos poderão ser utilizados na produção de plantas Bt, piramidadas ou não, visando ao manejo da resistência dos insetos praga / As an alternative to decrease the constant aggressions that the ecosystem has suffered due to the large amount of chemical products used in pest control, researches involving microorganisms able to promoting biological control have been intensified. Among these microorganisms the bacterium Bacillus thuringiensis has been stood out. This bacterium is characterized by the production of toxic proteins to representatives of several insect orders. In particular, the Vip3A proteins are in large study due to its specificity, and high active potential as an alternative to control of insect's resistance to Cry proteins. According to this, the aim of this work was to select from 1080 isolates in different Brazilian regions, those carrying vip3A genes and obtain the complete nucleotide sequence of the same. The standard strains B. thuringiensis var. kurstaki HD1, B. thuringiensis var. tolworthi HD125 and the isolate I187 had their DNA amplified with primers based on vip3A gene sequence described in database of B. thuringiensis, and from the amplicons obtained, the full sequence of nucleotides was determined, by the use of the strategy of primer walking. The protein Vip3Aa43 (GenBank: [HQ594534]) of HD1 strain showed to be 100% identical to Vip3Aa proteins already described. However, the proteins Vip3Aa42 (GenBank: [HQ587048]) of HD125 strain and Vip3Ag5 (GenBank: [HQ542193]) of the isolate I187 showed 99% similarity with the Vip3Aa35 and Vip3Ag2 proteins described, respectively, showing been two new Vip3A proteins due to amino acid substitutions occurred. The three vip3A genes obtained can be used in the production of Bt crops, pyramidal or not, aiming to resistance management of pest insects

Made available in DSpace on 2014-06-11T19:35:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-03-12Bitstream added on 2014-06-13T21:06:59Z : No. of bitstreams: 1 tavares_fm_dr_botfca.pdf: 538754 bytes, checksum: da1ec50d10839c5269a5a1390715eb52 (MD5) / Fundação de Apoio à Pesquisa Agrícola (FUNDAG) / Nematóides entomopatogênicos (NEPs) dos gêneros Heterorhabditis e Steinernema vem sendo utilizados para o controle de mosca-dos-fungos em diversos países. Esse estudo teve por objetivo avaliar diferentes isolados de NEPs, contra larvas de Bradysia mabiusi, em condições de laboratório e casa de vegetação. Nos testes de seleção de isolados, o nematóide Heterorhabditis indica apresentou melhor desempenho comparado a todos os demais isolados, proporcionando mortalidade do inseto de até 98%. Para o estudo em casa de vegetação, avaliou-se o efeito dos nematóides entomopatogênicos Heterorhabditis indica e Steinernema feltiae, nas dosagens de 10, 50 e 100 JI/cm2, em cultivo protegido de Chrysanthemum sp. Foram realizados 3 experimentos. Houve diferença significativa entre os tratamentos somente no terceiro teste, entretanto, nos três testes os nematóides demonstraram uma tendência de redução na população do inseto, em função do aumento das dosagens, o que sugere o efeito dos agentes no controle do inseto. Da mesma forma, em todos os testes, o nematóide H. indica apresentou maior redução na população da mosca-dos-fungos que o S. feltiae, confirmando sua maior eficiência com níveis de controle variando de 6 a 67,5% no primeiro ensaio, de 17 a 77,5% no segundo, e de 62,5 a 78,5% no terceiro. Os resultados revelam que H. indica apresenta potencial de uso para o controle da mosca-dos-fungos. / Entomopathogenic nematodes (EPNs) of the genus Heterorhabditis and Steinernema have been use against the fungus gnat in several countries. This study aimed to evaluate different strains of EPNs against larvae of Bradysia mabiusi, in laboratory and greenhouse conditions. At the screening of EPNs, the nematode H. indica showed better performance compared to the other nematodes, providing up to 98% mortality of the insect. To the study in greenhouse, evaluated the effects of the entomopathogenic nematodes Heterorhabditis indica e Steinernema feltiae, at dosage of 10, 50 and 100 IJ/cm2 in commercial growth of Chrysanthemum sp., in greenhouse conditions. Three experiments were accomplished. There were significative difference among the treatments only for the third experiment, mean while, in the three tests, the nematodes showed a tendency of reduction on the insect population according to the dosage increase, which suggest the effect of the agents on the insect control. In addition, in all the tests, the nematode H. indica showed higher reduction on the insect population compared to the S. feltiae, confirming its higher efficiency with control levels ranging from 6 to 67,5% in the first test, from 17 to 77,5% in the second, and from 62,5 to 78,5% in the third. The data show that H. indica has potential use for the controlling of the fungus gnat.

Made available in DSpace on 2014-06-11T19:27:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-05-06Bitstream added on 2014-06-13T19:55:58Z : No. of bitstreams: 1 santos_ps_me_jabo.pdf: 335704 bytes, checksum: 115114dd0becad3405d05e364da4f676 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Financiadora de Estudos e Projetos (FINEP) / Nos últimos anos o ataque da Dactylopius opuntiae, conhecida como cochonilha-do-carmim, à palma forrageira no Nordeste, tem causado grandes prejuízos principalmente na região da Paraíba, Pernambuco e Alagoas. Uma alternativa em desenvolvimento para o controle da praga é a seleção de fungos patogênicos e virulentos ao inseto. Neste estudo foi proposta a avaliação de produtos a serem usados como componentes da formulação de bioinseticidas, para o preparo de caldas a serem aplicadas no controle da cochonilha. Foi utilizado o isolado LCB63 de Beauveria bassiana previamente selecionado quanto à patogenicidade e virulência ao inseto. Avaliou-se a exposição do isolado a diferentes concentrações (0,005, 0,01, 0,05, 0,1, 0,5 e 1,0%) de surfactantes (Unitol L/20, Ultranex NP/100, Ultratan D, Surfon 3403, Surfon 5409, Sabão OMO®, Detergente neutro Ypê®) através do crescimento de colônias, germinação após exposição temporária aos produtos e a influência do balanço hidrofílico-lipofílico (HLB) na dispersão e viabilidade de conídios. Em outro experimento, avaliou-se a tolerância dos conídios do isolado à radiação solar e ultravioleta em fluxo laminar sob efeito de diferentes concentrações de fotoprotetores (Neo Heliopan AV, Neo Heliopan E1000, Oxibenzona, Amido e Leite) em diferentes períodos de exposição. Finalmente, verificou-se a mortalidade de ninfas de D. opuntiae utilizando-se formulações contendo os surfactantes e fotoprotetores que apresentaram os melhores resultados nos experimentos anteriores. Unitol L/20 e Ultranex NP/100 não apresentaram efeito deletério na formação de colônias, e Surfon 5409, detergente neutro Ypê® e sabão OMO®, apresentaram grande toxicidade ao fungo. Não houve interferência de Unitol L/20, Ultranex NP/100 e Ultratan D na germinação dos conídios nos primeiros 10 minutos de exposição aos surfactantes... / For the last years the attack of Dactylopius opuntiae know as Carmine cochineal to forage palm, in northern states of Brazil, have causes great prejudice, mainly in the regions of Paraíba, Pernambuco e Alagoas. One developing alternative for the control of this pest is the selection of pathogenic and virulent fungi to the insect. This study proposed the evaluation of products to be used as compounds in the formulation of bioinsecticides, for the preparation of broths applied in the control of cochineal. The isolate LCB63 of Beauveria bassiana previously selected for its pathogenicity and virulence to the insect, was used. The exposition of the isolate in different concentrations (0.005, 0.01, 0.05, 0.1, 0.5 and 1.0%) of surfactants (Unitol L/20, Ultranex NP/100, Ultratan D, Surfon 3403, Surfon 5409, OMO® washing powder, Ypê® neutral detergent) was evaluated through the colonies growth, germination after temporary exposition to the products and influence of the hydro-lipofilic balance (HLB) in the dispersion and viability of the conidia. Another experiment evaluated the tolerance of the conidia to solar radiation and ultraviolet in laminar flow hood, under the effect of different photoprotector concentrations (Neo Heliopan AV, Neo Heliopan E1000, Oxibenzona, starch and milk) with different exposition timing. Finally, the mortality of D. opuntiae in the nymph stage was verified by using the formulations containing the surfactants and the photoprotectors that demonstrated the best results in the previous experiments. Unitol L/20 and Ultranex NP/100 didn’t show a deleterious effect in the formation of the colonies, while Surfon 5409, Ypê® neutral detergent and OMO® washing powder showed great toxicity to the fungus. There was no interference of Unitol L/20, Ultranex NP/100 and Ultratan D in the conidia germination during the first ten minutes of exposition to the surfactants... (Complete abstract click electronic access below)