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Antibiotic associated diarrhea in horses : with special reference to Clostridium difficile /Gustafsson, Agneta, January 2004 (has links) (PDF)
Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2004. / Härtill 4 uppsatser.
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Evaluation of the pharmaceutical availability of erythromycin from topical formulations /Mandimika, Nyaradzo January 2008 (has links)
Thesis (M.Sc. (Pharmacy)) - Rhodes University, 2008
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Development of nanodispersions based on polyoxylglycerides to protect unstable molecules : Application to Helicobacter pylori treatment / Développement de nanodispersions à base de polyoxylglycerides pour la protection de molécules instables : Application au traitement d'Helicobacter pyloriTran, Le Tuyet Chau 25 November 2014 (has links)
L’utilisation des nanoparticules Janus pour la délivrance de médicaments suscite un grand intérêt depuis quelques années. Cependant, beaucoup d’aspects sont encore à comprendre dans la préparation de tels systèmes, notamment en présence de principes actifs.Le but de cette thèse était d’étudier des nanoparticules compartimentées Janus (JNP) constituées d’excipients lipidiques, et principalement de glycérides polyoxyéthylénés. Les objectifs étaient, d’une part, une meilleure compréhension de la structure et de la physico-chimie de ces objets puis, d’autre part, leur utilisation contre Helicobacter pylori (H. pylori).Ces JNP sont produites facilement par un procédé d’homogénéisation haute pression qui a permis de fabriquer des lots allant de 10 mL à 1 L. Elles ont été caractérisées par cryo-microscopie, calorimétrie, diffraction des rayons X, diffusion quasi-élastique de la lumière notamment et ont montrées une très grande stabilité physique sur plus d’un an. Les résultats les plus intéressants ont été obtenus avec une formulation contenant 20% de phase lipidique (Labrafil® M1944CS ou Labrafil® M2125CS) et 3% d’un mélange de tensioactifs (Gelucire® 50/13 – Phospholipon® 90G 2:1 en masse). Diverses expériences autour de la formulation et du procédé ont permis d’identifier certains paramètres critiques, notamment la nature de la phase lipidique, l’ordre d’incorporation des excipients ou le taux de diesters de PEG 1500.Des essais d’encapsulation dans ces JNP et de protection d’un principe actif instable en milieu acide, l’érythromycine, ont également été menés. Les nanodispersions formées, physiquement stable pendant au moins 6 mois, avaient une taille de l’ordre de 150 nm et une distribution de taille unimodale. Des tests in vitro ont montré que l’érythromycine encapsulé gardait son efficacité anti-microbienne et était plus stable en milieu acide. Par contre, les nanoparticules ont perdu leur compartimentation en présence d’érythromycineEnfin, des études ont été menées pour comprendre l’influence de l’incorporation de principes actifs (API) sur le procédé. Ainsi, il a été montré que l’ajout de caféine, chloroxylénol, quercétine et tricloasan dans la formulation n’altérait pas la morphologie des JNP contrairement à l’érythromycine et à la dioxybenzone dans une moindre mesure.L’ensemble de ces résultats a montré que les nanoparticules compartimentées Janus sont des systèmes prometteurs de transport et de protection de principes actifs. / The use of Janus nanoparticles (JNPs) as constituent of drug delivery formulations has been a topic of considerable interest for the past several years. However, the formation of vesicles and nanoparticle-associated drug/active are still unclear.The aim of the present study was a physicochemical characterization of lipid nanodispersions based on polyoxylglycerides, especially focused on lipid-based JNPs. The experiments should lead to a better understanding of structure and behavior of the very interesting carrier system on Helicobacter pylori (the abbreviation is H. pylori).The multicompartment lipid-based JNPs were produced easily at small (10 to 50 ml/batch) and large scale (1 liter/batch) by hot high pressure homogenization method. Samples were very well long-time stable for at least over 12 months with respect to particle size, DSC, XRD, and special particle morphology characteristics. Especially for particles containing 20% of lipid phase (Labrafil® M1944CS or Labrafil® M2125CS) and surfactant mixture of Gelucire® 50/13 – Phospholipon® 90G (2:1), strong adhesion could be observed in the studies by cryo-TEM technique. By changing the formulation components and process parameters, data showed that the formation of the multicompartment lipid-based JNPs depended on the using of suitable oil phase with the surfactant mixture and the method to produce the vesicles under the identified conditions.We also successfully prepared the stable nanodispersions to protect a labile antibiotic, erythromycin. The mean diameter of the ERY-loaded nanodispersions was found approximately 150 nm, and the size distribution was unimodal. The system was physically stable at room temperature for over six months. The test of antimicrobial activity in vitro on H. pylori showed that not only the preparation process did not reduce the antimicrobial activity of erythromycin, but also the stability of erythromycin was also improved in acidic environment.Furthermore, the properties of APIs loaded into the blank vesicles also affected their particle morphologies. We achieved the nanoparticles like JNPs when loading caffeine, chloroxylenol, quercetin, and triclosan into the vesicles. These results demonstrated that the multicompartment lipid-based JNPs are a promising carrier to protect unstable APIs and enhance their stability and solubility, despite the changes in the structure of the JNPs when incorporating with erythromycin or dioxybenzone.
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Characterization of 50S Ribosomal Subunit Assembly Inhibition in Erythromycin Treated <em>Escherichia coli</em> Cells.Usary, Jerry Edward 01 August 2000 (has links)
Erythromycin has long been recognized for its ability to inhibit protein synthesis by interfering with mRNA translation on the bacterial ribosome. We have recently shown that erythromycin also inhibits the assembly of the 50S ribosomal subunit in growing bacterial cells. The nature of this assembly inhibition has been investigated using 3H-uridine pulse-chase labeling of control and erythromycin treated E. coli cells.
Subunit assembly was examined by sucrose gradient centrifugation of labeled cell lysates. Normal assembly kinetics of subunit assembly were observed in control cells at 37°C. Formation of the 30S subunit was completed by 7.5 minutes and assembly of the 50S subunit was finished by 15 minutes after an unlabeled uridine chase. At 37°C, in the presence of erythromycin, 30S subunit assembly was unaffected but 50S assembly was greatly reduced. When the assembly kinetics were examined at 27°C, the assembly of both subunits was slowed and 30-32S precursor particle was seen to accumulate. This particle was found to bind 14C-erythromycin in vivo and in vitro.
RNase E has been implicated in the normal degradation and turnover of rRNA. A RNase E-mutant accumulated substantially more precursor to the 50S subunit than did control cells at either 37°C or 27°C. This precursor particle was also found to bind 14C-erythromycin. Specific 50S proteins and the 23S and 5SrRNAs were found in the 30S gradient region from lysates of cells grown at 27°C, confirming the presence of a 50S subunit precursor co-sedimenting with 30S subunits under these conditions. The precursor particle in the RNase E-mutant had a larger number of associated 50S proteins thandid the precursor from SK901. These data are consistent with our model of 50S subunit inhibition by erythromycin in which a fraction of 50S precursor particles undergo degraded.
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Eritromicina e tetraciclina resistenza in lactobacilli isolati da salami tipici del Nord Italia / Erythromycin and tetracycline resistant lactobacilli in the production of a typical dry sausage from the north of ItalyZONENSCHAIN, DANIELA 04 February 2009 (has links)
Lo scopo di questo lavoro di tesi era di stabilire la frequenza di lattobacilli resistenti all’eritromicina e alla tetraciclina sia nel prodotto finito che nella filiera di produzione di un salame DOP del Nord Italia. Il controllo della filiera produttiva comprendeva la pelle, la carne macinata e le feci di otto suini mentre il prodotto finito era analizzato a 0, 21, 35 e 45 giorni di stagionatura. Sono state isolate colonie di lattobacilli da terreni di coltura selettivi addizionati di antibiotici. Le specie antibiotico resistenti più frequentemente isolate erano Lactobacillus sakei, Lactobacillus curvatus and Lactobacillus plantarum. I geni che con maggior frequenza sono stati evidenziati nei ceppi isolati dalla filiera e nel prodotto finito erano tet(M) ed erm(B) mentre tet(W) ed erm(B) lo erano per i ceppi isolati dalle feci. Questo studio ha consentito di fornire dati circa la presenza e la diffusione di lattobacilli tetraciclina ed eritromicina resistenti in un salame tipico del Nord Italia. I lattobacilli antibiotico resistenti potrebbero trasferire ad altri batteri, patogeni o non patogeni, i geni responsabili della resistenza stessa. Alla luce di tutto questo l’80% dei salami analizzati possono essere considerati sicuri da questo punto di vista mentre il 20% può essere valutato come un prodotto a rischio anche se la situazione non è ancora preoccupante. / The scope of this study was to assess the frequency of erythromycin and tetracycline resistant lactobacilli in the production chain (skin, minced meat, and stools of eight swine, the natural casing, and the final product at days 0, 21, 35, and 45 of ripening) of a Protected Designation of Origin dry sausage from the North of Italy and in the end products. We isolated colonies of lactobacilli from selective medium supplemented with erythromycin or tetracycline and the most frequently antibiotic resistant species isolated were Lactobacillus sakei, Lactobacillus curvatus and Lactobacillus plantarum The most frequent resistance genes in process line strains were tet(M) and erm(B) while tet(W) and erm(B) were common in strains isolated from swine stools. This study provides evidence of the presence of tetracycline and, to a lesser extent, erythromycin resistant lactobacilli in fermented dry sausages produced in Northern Italy. Although these antibiotic resistant lactobacilli could serve as reservoir organisms, in our study 80% of salami could be considered as safe even though 20% could represent a border line situation regarding the possibility of transferring antibiotic resistant genes to pathogens.
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Επίδραση του ιοντικού περιβάλλοντος στη λειτουργία αντιβιοτικών που αναστέλλουν την πρωτεϊνική σύνθεσηΠετρόπουλος, Αλέξανδρος Δ. 23 December 2008 (has links)
Τα ριβοσώματα, οι μακρομοριακές μεταφραστικές μηχανές που είναι
υπεύθυνες για την πρωτεϊνική σύνθεση, αποτελούν έναν από τους
κυριότερους κυτταρικούς στόχους των αντιβιοτικών, που χορηγούνται για
αντιμικροβιακή θεραπεία. Μελέτες για περισσότερο από 40 χρόνια δείχνουν
ότι το κατάλληλο ιοντικό περιβάλλον (μονοσθενή, δισθενή κατιόντα και
πολυαμίνες) είναι απαραίτητο για τη σωστή ριβοσωματική λειτουργία, ενώ
παράλληλα επηρεάζει τις αλληλεπιδράσεις του με διάφορους προσδέτες.
Παρόλα αυτά η μοριακή βάση της επίδρασης του ιοντικού περιβάλλοντος στο
μηχανισμό δράσης των αντιβιοτικών δεν έχει ενδελεχώς μελετηθεί. Στόχος της
παρούσας διατριβής είναι η διερεύνηση του μηχανισμού δράσης
αντιβιοτικών που αναστέλλουν την πρωτεϊνική σύνθεση σε συνθήκες που
προσομοιάζουν με τις φυσιολογικές του κυττάρου και η μελέτη της επίδρασης
του ιοντικού περιβάλλοντος στη δράση αυτών. Τα αντιβιοτικά που
μελετήθηκαν ήταν: α) η βλαστισιδίνη, ως κλασικός αναστολέας της
πεπτιδυλοτρανσφεράσης (ΡΤάσης), β) το μακρολίδιο τυλοσίνη που
αναστέλλει την ΡΤάση, αλλά παράλληλα προσδένεται στην αρχή του τούνελ
εξόδου και παρεμποδίζει την πολυπεπτιδική αλυσίδα να εξέλθει από το
ριβόσωμα, και γ) τα μακρολίδια ερυθρομυκίνη (πρώτης γενεάς),
αζιθρομυκίνη (δεύτερης γενεάς) και τελιθρομυκίνη (μακρολίδιο τρίτης γενεάς
ή κετολίδιο), η δράση των οποίων έγκειται στην παρεμπόδιση του τούνελ
εξόδου. Εξίσου σημαντική φαίνεται να είναι η επίδραση των μακρολιδίων στη
συγκρότηση της 50S ριβοσωματικής υπομονάδας.
Ο μηχανισμός δράσης των αντιβιοτικών και η επίδραση του ιοντικού
περιβάλλοντος στη δράση τους έγινε αρχικά με κινητικές μελέτες. Το
πειραματικό σύστημα που χρησιμοποιήθηκε ήταν η αντίδραση
πουρομυκίνης, η οποία μας έδωσε τη δυνατότητα τιτλοδότησης των ενεργών
ριβοσωμάτων. Βάσει αυτού μελετήθηκαν τα αντιβιοτικά βλαστισιδίνη και
τυλοσίνη που αναστέλλουν άμεσα την ΡΤάση, ενώ για τη μελέτη των
υπολοίπων μακρολιδίων διεξήχθησαν πειράματα συναγωνιστικής αναστολής.
Ως γνωστό, τα μακρολίδια ερυθρομυκίνη, αζιθρομυκίνη και τελιθρομυκίνη μοιράζονται κοινές θέσεις πρόσδεσης στο ριβόσωμα με την τυλοσίνη. Έτσι,
για την εύρεση των σταθερών πρόσδεσης αυτών στο ριβόσωμα έγινε
συναγωνισμός με τυλοσίνη. Τα πειράματα συναγωνισμού
πραγματοποιήθηκαν, επωάζοντας το ριβόσωμα με μείγμα μακρολιδίου και
τυλοσίνης, και τιτλοδοτώντας την απομένουσα δραστικότητα του
ριβοσώματος με την αντίδραση πουρομυκίνης απομακρύνοντας την
περίσσεια αντιβιοτικών. Σε παράλληλα πειράματα, το ριβόσωμα
προεπωάστηκε αρχικά με το μακρολίδιο και στη συνέχεια προστέθηκε
τυλοσίνη, η οποία ανιχνεύει το εναπομείναν ριβοσωματικό σύμπλοκο. Επειδή
η σταθερά συγγένειας στη δεύτερη περίπτωση βρέθηκε μικρότερη (ισχυρότερη
πρόσδεση αντιβιοτικού) συμπεράναμε, ότι ο μηχανισμός πρόσδεσης του
αντιβιοτικού είναι βραδύς και ακολουθεί δυο στάδια. Βασιζόμενοι στις τιμές
των σταθερών συγγένειας σε πειράματα αναγέννησης του ριβοσωματικού
συμπλόκου από την απενεργοποιημένη μορφή του, προσδιορίστηκαν
ξεχωριστά όλες οι κινητικές παράμετροι που χαρακτηρίζουν την πρόσδεση
του αντιβιοτικού στο ριβόσωμα. Συγκρίναμε τις παραμέτρους αυτές και
γενικότερα την ισχύ πρόσδεσης των αντιβιοτικών στο ριβόσωμα σε πέντε
ιοντικές συνθήκες: (α) 4,5 mM Mg2+, 150 mM NH4+, (β) 4,5 mM Mg2+, 150 mM
NH4+, 100 μΜ σπερμίνη, (γ) 4,5 mM Mg2+, 150 mM NH4+, 50 μΜ σπερμίνη και
2 mM σπερμιδίνη, (δ) 4,5 mM Mg2+, 150 mM NH4+ ριβοσωματικό σύμπλοκο
φωτοσημασμένο με 100 μΜ ΑΒΑ-σπερμίνη, και (ε) 10 mM Mg2+, 100 mM
NH4+. Τα αποτελέσματα έδειξαν ότι οι πολυαμίνες βελτιώνουν την πρόσδεση
της βλαστισιδίνης, αλλά μειώνουν την πρόσδεση των μακρολιδίων. Η
επίδραση των ιόντων Mg2+ προσομοιάζει εκείνης των πολυαμινών, αλλά είναι
λιγότερο αποτελεσματική, αφού 100 μΜ σπερμίνης επιφέρουν μεγαλύτερη
αναστολή πρόσδεσης, από ότι 10 mM Mg2+.
Για να ερμηνευτεί σε μοριακό επίπεδο η επίδραση της σπερμίνης στη
συγγένεια των αντιβιοτικών έναντι του ριβοσώματος, οι θέσεις πρόσδεσης
των πολυαμινών στο ριβόσωμα προσδιορίστηκαν με φωτοσήμανση
συγγένειας, χρησιμοποιώντας ως μοριακό ανιχνευτή ένα φωτοδραστικό
ανάλογο της σπερμίνης, την ΑΒΑ-σπερμίνη. Οι θέσεις αυτές αποκάλυψαν ότι οι πολυαμίνες προσδένονται σε γειτονικές θέσεις προς τα αντιβιοτικά,
επηρεάζοντας την τοπική διαμόρφωση και το φορτίο.
Επιβεβαίωση του μηχανισμού δράσης και της επίδρασης των
πολυαμινών έγινε με ανάλυση αποτυπώματος. Σύμφωνα με την τεχνική αυτή,
τα μακρολίδια προσδενόμενα στο ριβόσωμα προστατεύουν ορισμένα
νουκλεοτίδια από την επίδραση χημικών τροποποιητών. Τα αποτελέσματα
έδειξαν ότι τα αντιβιοτικά διέρχονται μια ενδιάμεση κατάσταση πρόσδεσης
στο ριβόσωμα δεσμευόμενα αρχικά στην είσοδο του τούνελ εξόδου και στη
συνέχεια εισχωρώντας βαθύτερα σε αυτό. Η φύση της ενδιάμεσης κατάστασης
εξαρτάται από τα ιδιαίτερα χαρακτηριστικά του κάθε μακρολιδίου. Η
επίδραση των πολυαμινών στο μηχανισμό πρόσδεσης ελέγχθηκε
επαναλαμβάνοντας τα πειράματα χημικής προστασίας παρουσία αυτών. Τα
αποτελέσματα έδειξαν ότι η μείωση της πρόσδεσης των μακρολιδίων στο
ριβόσωμα επιτελείται κυρίως μέσω της επίδρασης των πολυαμινών στη
δέσμευση του υδρόφοβου λακτονικού δακτυλίου. Τα ιδιαίτερα
χαρακτηριστικά του κάθε αντιβιοτικού επηρεάζουν ποικιλοτρόπως το μέγεθος
της επίδρασης αυτής.
Στο τελευταίο κομμάτι της διατριβής μελετήθηκε η ισχύς των
μακρολιδίων, υπολογίζοντας την αναστολή που προκαλούν στο συζευγμένο
σύστημα μεταγραφής-μετάφρασης του γονιδίου της GFP πρωτεΐνης, και τα
αποτελέσματα επιβεβαίωσαν τα κινητικά δεδομένα πρόσδεσης των
μακρολιδίων στο ριβόσωμα-στόχο. Σε υψηλή συγκέντρωση ιόντων Mg2+ η
τυλοσίνη έχει μεγαλύτερη ισχύ, ενώ σε χαμηλή συγκέντρωση ιόντων απουσία
ή παρουσία πολυαμινών η αζιθρομυκίνη. Η τελιθρομυκίνη παρουσίασε τη
χαμηλότερη ισχύ πρόσδεσης στο ριβόσωμα και αναστολής της πρωτεϊνικής
σύνθεσης. Επιπρόσθετα, ελέγχθηκε πιθανή επίδραση των μακρολιδίων στην
πρόσδεση των υποστρωμάτων (tRNAs) στην Α-, Ρ- και Ε- θέση του
ριβοσώματος, στη μετατόπιση αυτών από την Α- στην Ρ- θέση και στη
μεταφραστική πιστότητα του ριβοσώματος. Βρήκαμε ότι τα μακρολίδια δεν
μπορούν να επηρεάσουν αυτά τα στάδια της ριβοσωματικής λειτουργίας. / Ribosomes, the macromolecular translating machines responsible for
protein biosynthesis, are the most common targets for many antibacterial
agents. Experiments for more than 40 years have demonstrated that a distinct
ionic environment (monovalent, divalent cations and polyamines) is essential
for ribosomal functions and their interactions with the ligands. Nevertheless,
the molecular basis of the ionic environment’s influence on antibiotic
mechanism of action has never been precisely elucidated.
The aim of this thesis was first to investigate the mechanism of action
of several antibiotics –inhibitors of protein synthesis, under ionic conditions
close to the cell environment and second, to clarify the role of the ionic
environment on their mechanism of action. The antibiotics studied were: a)
blasticidin-S, a classic inhibitor of peptidyl tranferase (PTase) activity, b)
tylosin which inhibits PTase, but in parallel binds at the entrance of exit
tunnel and blocks the passage of the nascent polypeptide chain, and c)
erythromycin (a first generation macrolide), azithromycin (a second
generation macrolide), and telithromycin (a third generation macrolide,
ketolide), that blocks the exit tunnel.
The mechanism of action of antibiotics and the influence of ionic
environment on antibiotic potency was studied primarily with kinetic
methods. The experimental procedure was based on the puromycin reaction,
performed under conditions allowing the estimation of the catalytic rate
constant. Using this experimental approach we studied the mechanism of
action of blasticidin and tylosin which directly inhibit PTase. For studying
the other macrolides, experiments employing competitive kinetics were
performed. Erythromycin, azithromycin and telithromycin share common
binding sites on ribosomes with tylosin. Thus, to estimate the kinetic
constants of their interactions with ribosomes, competitive kinetic
experiments were carried out in the presence of tylosin. Namely, a posttranslocation
ribosomal complex formed from Escherichia coli 70S ribosomes
bearing tRNAPhe at the E-site and AcPhe-tRNA at the P-site (complex-C) was incubated with a mixture of each macrolide and tylosin for the desired time
intervals. The rest of ribosomal activity was titrated by the puromycin
reaction. In parallel experiments, complex-C was pre-incubated with each one
of the macrolides and then reacted with tylosin. The rest of complex-C activity
was again titrated with the puromycin reaction. Since the affinity constant
obtained by the second series of experiments was less than that obtained by
the first series of experiments, we concluded that the mechanism of action of
antibiotics follows a slow onset inhibition process, which includes two steps.
Based on secondary plots and on kinetic plots derived from regeneration of
complex-C, we measured the kinetic parameters participating in the kinetic
model. Thus, the potency of each antibiotic was determined under five
different ionic conditions: (a) 4,5 mM Mg2+, 150 mM NH4+, (b) 4,5 mM Mg2+,
150 mM NH4+, 100 μΜ spermine, (c) 4,5 mM Mg2+, 150 mM NH4+, 50 μΜ
spermine and 2 mM spermidine, (d) 4,5 mM Mg2+, 150 mM NH4+, and
ribosomal complex photolabelled with 100μΜ ΑΒΑ-spermine, and (e) 10 mM
Mg2+, 100 mM NH4+. Processing of the data led us to the conclusion that
polyamines and Mg2+ ions increase the potency of blasticidin, but decrease the
potency of macrolides.
To explain the diverse action of polyamines and of the ionic
environment in general on antibiotic potency, the binding sites of spermine in
ribosomes were localized by photoaffinity labeling, using a photoactive
analogue of spermine, ABA-spermine. These experiments revealed that
polyamines bind at the vicinity of antibiotics, influencing the ionic charge and
the local conformation of rRNA.
Confirmation of the macrolide mechanism of action and verification of
the influence of polyamines on their potency was achieved by footprinting
analysis. According to this technique, macrolides bind to ribosomes and
protect specific nucleotides from modification by chemical reagents like DMS,
CMCT and kethoxal. The results demonstrated that the antibiotics (I) form an
encounter complex with complex-C (CI), in which the antibiotics occupy the
entrance of the exit tunnel. This intermediate complex is then isomerized slowly to a tighter complex (C*I) with which antibiotics move deeply in the
exit tunnel. The exact interactions stabilizing the intermediate complex
depend on the characteristic groups of each macrolide. The influence of
polyamines was checked by repeating the experiment in the presence of
polyamines. The results showed that polyamines reduce the macrolide
binding to ribosomes, by affecting mainly the interactions of the hydrophobic
lactone ring with the ribosome. The special characteristic groups of each
macrolide affect the polyamine action. The potency of macrolides action was
also estimated using a coupled transcription-translation system for GFP
expression. The results obtained were consistent with those produced by
kinetic analysis. In addition, we check for possible macrolide effects on tRNA
binding at the A-, P- and E- sites of the ribosome, on translocation, and on
translational fidelity. No strong effects were identified excluding the
macrolide from these ribosomal functions.
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Macrolide resistance and its linkage to tetracycline resistance /Chung, Whasun Oh. January 1999 (has links)
Thesis (Ph. D.)--University of Washington, 1999. / Vita. Includes bibliographical references (leaves 112-144).
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Avaliação da penetração de agentes antimicrobianos em biofilme de staphylococcus spp. e pseudomonas aeruginosa : considerações físico-químicas / Evaluation of the penetration of antimicrobial agents on biofilm of staphylococcus spp. and pseudomonas aeruginosa : physical-chemical considerationsPinto, Camille Catani Ferreira January 2011 (has links)
O advento do uso de cateteres venosos centrais na prática médica trouxe muitos benefícios aos pacientes, porém está relacionado a um aumento na incidência de infecções por microrganismos multirresistentes. Além disso, freqüentemente ocorre colonização por bactérias produtoras de biofilme. Estes microrganismos se aderem ao material abiótico desses dispositivos intravenosos, ficando protegidos sob a matriz exopolissacarídica do biofilme. Isso faz com que sistema imunológico e antimicrobianos sejam incapazes de ter sua ação plena e, muitas vezes, não atingem os microrganismos mais internos. O motivo deste insucesso é porque muitos desses agentes biológicos e farmacológicos apresentam propriedades físico-químicas incompatíveis com a penetração nesta matriz. Com o objetivo de determinar quais antimicrobianos são mais adequados para uso quando o microrganismo é produtor de biofilme e quais as propriedades físico-químicas que estão diretamente relacionadas à penetração do antimicrobiano na matriz polissacarídica, utilizou-se método colorimétrico com cristal violeta em microplacas modificado para obtenção de concentração inibitória mínima em biofilme (MBIC) e método já padronizado para concentração inibitória mínima (MIC). Para isso foram testados 10 antimicrobianos em Staphylococcus spp.: rifampicina, azitromicina, claritromicina, eritromicina, levofloxacino, gentamicina, doxiciclina, cloranfenicol, clindamicina e vancomicina. Para Pseudomonas aeruginosa foram testados os mesmos, exceto rifampicina e vancomicina. A discrepância entre MIC e MBIC foi muito grande para vários fármacos, mostrando a necessidade de se avaliar estes parâmetros antes do início da farmacoterapia para uma escolha correta, especialmente em hospitais. Os fármacos que apresentaram melhores resultados foram a rifampicina e os macrolídeos, enquanto que os menos efetivos foram vancomicina e clindamicina. Isso foi atribuído ao perfil lipofílico, porém com alguma solubilidade em água das melhores moléculas. Em contra ponto, a elevada área polar, complexidade e massa molar foram características negativas para a penetração em biofilme, resultando numa ineficácia para essas moléculas. Além disso, também foi avaliado o tratamento de polímeros plásticos com EDTA, obtendo-se redução significativa da produção de biofilme nas placas tratadas com o agente químico. / The use of central venous catheters in medicine has brought benefits to the patients and represents a great advance in clinical practice, while on the other hand this device is related to an increase in the incidence of infections caused by multiresistant pathogens. Furthermore, frequently, the catheters get colonized by biofilm producing bacteria. These microorganisms adhere to the abiotic material of the catheters keeping themselves protected underneath the exopolysaccharide matrix of biofilm, this way the immune system and antimicrobials are incapable to fulfill their action and, many times, are unable to reach internal bacteria. This fact is explained by the fact that many of the biological and pharmacological agents have physical-chemical properties incompatible with the penetration into the matrix. Aiming to determine which antimicrobials are suitable for using when dealing with a biofilm producing microorganism and which physical-chemical properties are directly related to the agent penetration into the polysaccharide matrix, we used colorimetric method with crystal violet to obtain biofilm minimum inhibitory concentration (MBIC) and the already standardized method for minimum inhibitory concentration (MIC). To accomplish these 10 antimicrobials were tested in Staphylococcus spp.: rifampin, azithromycin, clarithromycin, erythromycin, levofloxacin, gentamicin, doxycycline, chloramphenicol, clindamycin and vancomycin. For Pseudomonas aeruginosa all antimicrobials except for rifampin and vancomycin were included. There was a great difference between MIC and MBIC for many drugs, showing the need to evaluate these parameters before beginning treatment. The drugs with better results were rifampin and macrolides, while the worse were vancomycin and clindamycin, which can be attributed to the lipophilic profile with some water solubility present in the molecules with better results. The characteristics associated with poor penetration into biofilm were high polar surface area, complexity e molecular weight. Furthermore, the previous treatment of the plastic polymers with EDTA was accessed resulting in statistically significant reduction of biofilm production.
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Avaliação da penetração de agentes antimicrobianos em biofilme de staphylococcus spp. e pseudomonas aeruginosa : considerações físico-químicas / Evaluation of the penetration of antimicrobial agents on biofilm of staphylococcus spp. and pseudomonas aeruginosa : physical-chemical considerationsPinto, Camille Catani Ferreira January 2011 (has links)
O advento do uso de cateteres venosos centrais na prática médica trouxe muitos benefícios aos pacientes, porém está relacionado a um aumento na incidência de infecções por microrganismos multirresistentes. Além disso, freqüentemente ocorre colonização por bactérias produtoras de biofilme. Estes microrganismos se aderem ao material abiótico desses dispositivos intravenosos, ficando protegidos sob a matriz exopolissacarídica do biofilme. Isso faz com que sistema imunológico e antimicrobianos sejam incapazes de ter sua ação plena e, muitas vezes, não atingem os microrganismos mais internos. O motivo deste insucesso é porque muitos desses agentes biológicos e farmacológicos apresentam propriedades físico-químicas incompatíveis com a penetração nesta matriz. Com o objetivo de determinar quais antimicrobianos são mais adequados para uso quando o microrganismo é produtor de biofilme e quais as propriedades físico-químicas que estão diretamente relacionadas à penetração do antimicrobiano na matriz polissacarídica, utilizou-se método colorimétrico com cristal violeta em microplacas modificado para obtenção de concentração inibitória mínima em biofilme (MBIC) e método já padronizado para concentração inibitória mínima (MIC). Para isso foram testados 10 antimicrobianos em Staphylococcus spp.: rifampicina, azitromicina, claritromicina, eritromicina, levofloxacino, gentamicina, doxiciclina, cloranfenicol, clindamicina e vancomicina. Para Pseudomonas aeruginosa foram testados os mesmos, exceto rifampicina e vancomicina. A discrepância entre MIC e MBIC foi muito grande para vários fármacos, mostrando a necessidade de se avaliar estes parâmetros antes do início da farmacoterapia para uma escolha correta, especialmente em hospitais. Os fármacos que apresentaram melhores resultados foram a rifampicina e os macrolídeos, enquanto que os menos efetivos foram vancomicina e clindamicina. Isso foi atribuído ao perfil lipofílico, porém com alguma solubilidade em água das melhores moléculas. Em contra ponto, a elevada área polar, complexidade e massa molar foram características negativas para a penetração em biofilme, resultando numa ineficácia para essas moléculas. Além disso, também foi avaliado o tratamento de polímeros plásticos com EDTA, obtendo-se redução significativa da produção de biofilme nas placas tratadas com o agente químico. / The use of central venous catheters in medicine has brought benefits to the patients and represents a great advance in clinical practice, while on the other hand this device is related to an increase in the incidence of infections caused by multiresistant pathogens. Furthermore, frequently, the catheters get colonized by biofilm producing bacteria. These microorganisms adhere to the abiotic material of the catheters keeping themselves protected underneath the exopolysaccharide matrix of biofilm, this way the immune system and antimicrobials are incapable to fulfill their action and, many times, are unable to reach internal bacteria. This fact is explained by the fact that many of the biological and pharmacological agents have physical-chemical properties incompatible with the penetration into the matrix. Aiming to determine which antimicrobials are suitable for using when dealing with a biofilm producing microorganism and which physical-chemical properties are directly related to the agent penetration into the polysaccharide matrix, we used colorimetric method with crystal violet to obtain biofilm minimum inhibitory concentration (MBIC) and the already standardized method for minimum inhibitory concentration (MIC). To accomplish these 10 antimicrobials were tested in Staphylococcus spp.: rifampin, azithromycin, clarithromycin, erythromycin, levofloxacin, gentamicin, doxycycline, chloramphenicol, clindamycin and vancomycin. For Pseudomonas aeruginosa all antimicrobials except for rifampin and vancomycin were included. There was a great difference between MIC and MBIC for many drugs, showing the need to evaluate these parameters before beginning treatment. The drugs with better results were rifampin and macrolides, while the worse were vancomycin and clindamycin, which can be attributed to the lipophilic profile with some water solubility present in the molecules with better results. The characteristics associated with poor penetration into biofilm were high polar surface area, complexity e molecular weight. Furthermore, the previous treatment of the plastic polymers with EDTA was accessed resulting in statistically significant reduction of biofilm production.
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Avaliação da penetração de agentes antimicrobianos em biofilme de staphylococcus spp. e pseudomonas aeruginosa : considerações físico-químicas / Evaluation of the penetration of antimicrobial agents on biofilm of staphylococcus spp. and pseudomonas aeruginosa : physical-chemical considerationsPinto, Camille Catani Ferreira January 2011 (has links)
O advento do uso de cateteres venosos centrais na prática médica trouxe muitos benefícios aos pacientes, porém está relacionado a um aumento na incidência de infecções por microrganismos multirresistentes. Além disso, freqüentemente ocorre colonização por bactérias produtoras de biofilme. Estes microrganismos se aderem ao material abiótico desses dispositivos intravenosos, ficando protegidos sob a matriz exopolissacarídica do biofilme. Isso faz com que sistema imunológico e antimicrobianos sejam incapazes de ter sua ação plena e, muitas vezes, não atingem os microrganismos mais internos. O motivo deste insucesso é porque muitos desses agentes biológicos e farmacológicos apresentam propriedades físico-químicas incompatíveis com a penetração nesta matriz. Com o objetivo de determinar quais antimicrobianos são mais adequados para uso quando o microrganismo é produtor de biofilme e quais as propriedades físico-químicas que estão diretamente relacionadas à penetração do antimicrobiano na matriz polissacarídica, utilizou-se método colorimétrico com cristal violeta em microplacas modificado para obtenção de concentração inibitória mínima em biofilme (MBIC) e método já padronizado para concentração inibitória mínima (MIC). Para isso foram testados 10 antimicrobianos em Staphylococcus spp.: rifampicina, azitromicina, claritromicina, eritromicina, levofloxacino, gentamicina, doxiciclina, cloranfenicol, clindamicina e vancomicina. Para Pseudomonas aeruginosa foram testados os mesmos, exceto rifampicina e vancomicina. A discrepância entre MIC e MBIC foi muito grande para vários fármacos, mostrando a necessidade de se avaliar estes parâmetros antes do início da farmacoterapia para uma escolha correta, especialmente em hospitais. Os fármacos que apresentaram melhores resultados foram a rifampicina e os macrolídeos, enquanto que os menos efetivos foram vancomicina e clindamicina. Isso foi atribuído ao perfil lipofílico, porém com alguma solubilidade em água das melhores moléculas. Em contra ponto, a elevada área polar, complexidade e massa molar foram características negativas para a penetração em biofilme, resultando numa ineficácia para essas moléculas. Além disso, também foi avaliado o tratamento de polímeros plásticos com EDTA, obtendo-se redução significativa da produção de biofilme nas placas tratadas com o agente químico. / The use of central venous catheters in medicine has brought benefits to the patients and represents a great advance in clinical practice, while on the other hand this device is related to an increase in the incidence of infections caused by multiresistant pathogens. Furthermore, frequently, the catheters get colonized by biofilm producing bacteria. These microorganisms adhere to the abiotic material of the catheters keeping themselves protected underneath the exopolysaccharide matrix of biofilm, this way the immune system and antimicrobials are incapable to fulfill their action and, many times, are unable to reach internal bacteria. This fact is explained by the fact that many of the biological and pharmacological agents have physical-chemical properties incompatible with the penetration into the matrix. Aiming to determine which antimicrobials are suitable for using when dealing with a biofilm producing microorganism and which physical-chemical properties are directly related to the agent penetration into the polysaccharide matrix, we used colorimetric method with crystal violet to obtain biofilm minimum inhibitory concentration (MBIC) and the already standardized method for minimum inhibitory concentration (MIC). To accomplish these 10 antimicrobials were tested in Staphylococcus spp.: rifampin, azithromycin, clarithromycin, erythromycin, levofloxacin, gentamicin, doxycycline, chloramphenicol, clindamycin and vancomycin. For Pseudomonas aeruginosa all antimicrobials except for rifampin and vancomycin were included. There was a great difference between MIC and MBIC for many drugs, showing the need to evaluate these parameters before beginning treatment. The drugs with better results were rifampin and macrolides, while the worse were vancomycin and clindamycin, which can be attributed to the lipophilic profile with some water solubility present in the molecules with better results. The characteristics associated with poor penetration into biofilm were high polar surface area, complexity e molecular weight. Furthermore, the previous treatment of the plastic polymers with EDTA was accessed resulting in statistically significant reduction of biofilm production.
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