Thesis (Ph. D.)--University of Rhode Island, 2005. / Typescript. Includes bibliographical references (leaves 129-143).

In available literature there is no recommended set of microsatellite markers in the improvement of cabbage cultivars (Brassica oleracea conv. capitata) to distinguish F1 hybrids and self-pollinated plants with a parental genotype. That is why microsatellite markers used to other Brassicas had been searched in this work. Tested microsatellite markers were studied for their ability of distinguishing F1 hybrids in seven cabbage cultivars. Two of 23 microsatellite markers were able to distinguish F1 hybrids in six cabbage cultivars. No microsatellite marker was able to distinguish F1 hybrid and parental genotypes of one cultivar, 'A'.

This thesis offers the first in-depth analysis of the network of Puerto Rican community activism in the Lower East Side from 1964 to 2001. The community of Loisaida organized itself to fight against postwar urban deindustrialization, housing disinvestment, and gentrification, which threatened to displace an entire generation of Puerto Ricans who migrated to this New York neighborhood and tried to make it their home. Using an amalgam of unprocessed organizational archives, oral histories, ephemera, and neighborhood publications, this project recreates the history of community action in Loisaida. Focusing on key institutions and community groups that mobilized residents and built a lasting activist network, this thesis demonstrates how community groups pioneered a methodology for more sustainable community activism. These activists turned Loisaida into their laboratory, constantly experimenting with and adapting new strategies to put up a solid defense against absentee landlords, greedy developers, opportunist politicians, and an era of increased policing of urban space. The interplay of community activism, urban politics, and Puerto Rican history in Loisaida provides three crucial insights: (1) the need for grassroots organizations to adapt their activism to the changing needs of the community, (2) the creativity of urban communities to transform and design their immediate environment, and (3) the key strategies that enable activists to develop campaigns to their full potential. By uncovering these insights, this thesis raises new and challenging questions about the nature of sustained neighborhood activism at a major transitional phase in United States urban history: the shift from 1960s antipoverty programs to 1980s neoliberal policies. It shows the ingenuity and strength of activists who confronted this shift in the socio-political urban landscape by devising strategies to continue serving the residents of Loisaida. In 2017, the same community leaders who mobilized residents in the early 1960s are still marching on City Hall to demand the return of their former headquarters.

Made available in DSpace on 2014-06-12T15:53:32Z (GMT). No. of bitstreams: 2 arquivo5149_1.pdf: 3970263 bytes, checksum: 9d5eb7ba6122760de6e5e455fb706f21 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2005 / Bactérias do gênero Yersinia possuem um sistema de secreção tipo III, responsável pela translocação de fatores protéicos conhecidos como Yops, para o interior de células eucarióticas do hospedeiro. A proteína YopH é uma tirosina fosfatase que atua desfosforilando moléculas sinalizadoras e impedindo a fagocitose em macrófagos. Para uma secreção eficiente, ela necessita da presença da chaperona SycH que permite o seu reconhecimento pela maquinaria de secreção. Com a finalidade de analisar a expressão da proteína YopH em E. coli, foi construído um promotor híbrido formado pelo promotor lac de Escherichia coli seguido do promotor yopH de Yersinia enterocolitica. Este promotor foi capaz de direcionar uma forte expressão da YopH, em E. coli, e uma síntese ótima de YopH foi observada com o aumento da temperatura (37oC) e em culturas em fase de crescimento. A expressão da YopH foi investigada em linhagens defectivas de E. coli para as proteínas homólogas às histonas, Hha e H-NS. A expressão foi significantemente maior a 24oC que a 37oC, na ausência de H-NS, mas não na ausência de Hha. Estes resultados são compatíveis com o papel da H-NS na regulação da atividade do promotor yoph, possivelmente através da presença de curvas em sua estrutura. Para determinar se a presença da chaperona SycH influenciaria na expressão da YopH, em E. coli, a sequência codificadora para esta proteína foi amplificada e clonada no plasmídio pBAD33. Foi observada a expressão da SycH em culturas de E. coli, apenas na presença de arabinose. A co-expressão das proteínas YopH e SycH, em EPEC, revelou uma redução na expressão da YopH, na presença de arabinose. O mesmo foi observado em culturas de EPEC contendo apenas o plasmídio pTZ/YopH, onde concentrações variadas de arabinose e glicose estariam influenciando a expressão da YopH. Para verificar o potencial da proteína YopH no diagnóstico da peste, diversos soros de pacientes suspeitos de peste e coelhos imunizados com Y. pestis foram testados por Western-blot. Não houve reconhecimento da YopH nos soros testados

Tendões são descritos como tecido conjuntivo denso modelado que inserem os músculos aos ossos. Sua principal função é servir como tecido de transição das forças contráteis geradas pelos músculos aos ossos, podendo assim gerar movimentos. Entre todos os tendões, o calcâneo é um dos mais frequentemente lesados. As lesões ocorrem principalmente em homens e são mais frequentes entre a terceira e quarta década de vida. Dentre os vários métodos de sutura existentes, do ponto de vista clínico, a de Kessler-Tajima com os nós entre os cotos é muito utilizada por evitar o estrangulamento da microcirculação. Após a cirurgia o paciente retorna ao trabalho após 85 dias, em média, podendo atingir até 270 dias. O objetivo do presente estudo foi testar a eficiência da aplicação da proteína biocompatível F1, uma proteína extraída a partir do látex natural da Hevea brasiliensis, sobre tendões lesados, buscando uma melhora no reparo, assim como, a influência da sutura-U e de Kessler-Tajima. Alterações musculares decorrentes da lesão no tendão também foram avaliadas. Para isso, utilizamos ratos Sprague Dawley machos de 3 meses, que foram submetidos a tenotomia completa, a lesão foi corrigida com ambas as suturas, seguido da aplicação da proteína e avaliados após duas e quatro semanas. As técnicas utilizadas para análises no tendão foram as de microscopia de luz, microscopia eletrônica de varredura e transmissão, bem como, a síntese de colágeno tipos I e III, TIMP-1 e 2, MMP-2 e 9 por western blot. Para analisar as alterações musculares, a expressão dos genes MuRF1 e Atrogin1 foram quantificadas por RT-qPCR, seguido de análises de contração muscular máxima e teste de fadiga. Os resultados sugerem uma melhora no reparo do tendão no grupo que utilizamos a sutura-U associada à proteína após quatro semanas, devido ao aumento na síntese de colágeno tipo I e a diminuição de MMP-9, assim como, a capacidade de contração muscular máxima retorna aos níveis do grupo controle. A sutura-U também influencia menos na fadiga muscular. / Tendons are described as dense modeled connective tissue which insert the muscles to the bones. Its main function is to serve as transition tissue of the contractile forces generated by muscles to the bones, and thus generate movements. Among all the tendons, the calcaneal is the most frequently injured one. The lesions occur mainly in men and are more frequent between the third and fourth decade of life. Among the several existing suture methods, from a clinical point of view, the Kessler-Tajima with the nodes between the stumps is used to a great extent in order to avoid the microcirculations strangling. After surgery, the patient returns to work after 85 days, on average, and can reach up to 270 days. The objective of this study was to test the efficiency of the application of biocompatible F1 protein, a protein extracted from natural latex from the Hevea brasiliensis, on injured tendons, seeking an improvement in the repair, as well as the influence of the suture-U and Kessler-Tajima. Muscle changes arising from the tendon injury were also evaluated. For this reason, Sprague Dawley male rats at 3 mounths-old were used, which underwent complete tenotomy, the lesion was corrected with both sutures, followed by the application of protein and evaluated after two and four weeks. The techniques used for analyzes in the tendon were the light microscopy, scanning electron microscopy and transmission, as well as the synthesis of collagen types I and III, TIMP-1, -2, MMP-2, and -9 by Western blot. In order to analyze the muscular alterations, the expression of genes MuRF1 and Atrogin1 were quantified by RT-qPCR, followed by analysis of maximum muscle contraction and fatigue testing. The results suggest an improvement in the tendon repair in the group that the suture-U was used associated with protein after four weeks, due to the increase in the synthesis of collagen type I and the decrease of MMP-9, as well as the ability of maximum muscle contraction to return to the levels of the control group. The suture-U also influences on less muscle fatigue.

La F1 Fo ATP synthase est un complexe enzymatique localisé au sein de la membrane interne mitochondriale qui utilise le gradient électrochimique en protons formé par la chaîne respiratoire pour synthétiser de l'ATP à partir d'ADP et de Pi. Cette enzyme conservée de la levure S. cerevisiae aux cellules de mammifères s'organise dans les membranes internes mitochondriales sous forme de structures supramoléculaires d'ATP synthases. Chez la levure, il est aujourd'hui parfaitement identifiée que cette organisation nécessite la présence de deux sous-unités accessoires de l'enzyme : les sous-unités e et g.Les travaux présentés dans ce manuscrit visaient à étudier l'implication des sous-unités e et g dans les mécanismes de dimérisation et d'oligomérisation des ATP synthases ainsi que dans la morphogénèse des crêtes mitochondriales chez la levure S. cerevisiae et dans les cellules humaines en culture.Chez la levure, l'étude réalisée nous a permis de déterminer la stœchiométrie des sous-unités e et g, élément indispensable à la modélisation de l'agencement des sous-unités membranaires de l'enzyme dans la membrane interne mitochondriale.Dans les cellules humaines en culture, nous avons pu établir que les sous-unités e et g participent à la stabilité des dimères d'ATP synthases. Cependant l'implication de ces sous-unités dans la stabilité de l'enzyme semble différente des observations effectuées dans les cellules de levure / The F1Fo ATP synthase is an enzymatic complex embedded in the inner mitochondrial membrane which use the electrochemical proton gradient generated by the phosphorylation oxydative pathway to synthesize ATP from ADP and inorganic phosphate. This enzyme is conserved from yeast to mammalian cells and displays supramolecular organization in the inner mitochondrial membrane. In yeast, it is actually well-known that the supramolecular assembly required two accessory subunits : e and g subunits.The present work was realized to understand the involvement of subunits e and g in dimerization and oligomerization of mitochondrial ATP synthases as well as their effect on mitochondrial inner membrane morphogenesis in yeast S. cerevisiae and human cultured cells.In yeast, this study led us to determine subunits e and g stoechiometry, which was cruelly missing to establish a model of the ATP synthases membranous subunits layout in the inner mitochondrial membrane.In human cells, we have demonstrated that subunits e and g are implicated in ATP synthase dimer stabilization. However, their involvement in this stabilization seems to be quietly different of what have been observed in yeast cells.

Many of the 10 Central European candidate member countries for EU accession entered into the transition period with strongly undervalued exchange rates to stimulate exports and protect domestic industries. However, this policy was not maintained. During 1993-1995 real currency appreciation increased competitive pressure by foreign firms. To protect domestic firms governments applied high third country tariffs, temporary import taxes, and numerous administrative barriers to trade. As countervailing pressure by the EU and the USA increased and current account deficits soared in 1996 and 1997, the CE-10 more and more brought exchange rate policies in line with the changes in purchasing power parity. However, petty protection and harassment of importers prevails. (author's abstract) / Series: EI Working Papers / Europainstitut

This diploma thesis deals with testing of hybrid seeds genetic purity in three varieties of head cabbage (Brassica oleracea var. capitata L.). The suitability of SSR primers chosen according to an earlier screening carried out as a part of a research project in the workplace of Mendeleum and hybrid seeds genetic purity in head cabbage by SSR molecular method was tested. Among the total of 8 SSR primers screened, only 5 could produce both parent specific markers and therefore they were able to differentiate hybrid offspring and its corresponding parental lines, by which crossbreeding they originated. For each variety, 70 samples of hybrid offspring and 2 samples of parental lines were tested always in two sets. By using these primers, the genetic purity of the variety D - F1 was determinated as 100% and of the varieties H - F1 and T - F1 as 98.57%. The results of this thesis have the potential to be used in practice by a commercial company that has provided the seed samples for testing.

I detta projekt har ett koncept garage för Red Bull Racing F1 Team tagits fram inför säsongen 2023. Designutmaningen i projektet var att studera nuvarande koncept och hitta lösningar för ett fortsatt flow för personal men också ett stort fokus på hur man på bästa sätt kan hitta en designlösning på hur man kan visa upp varumärket och bilarna inför 2023 och framtiden. Uppgiften togs fram av mentorn från Red Bulls sida på detta projekt, Stefan Bridges, head of creative design. Under hela samarbetet har kontakten med Red Bull vart stor och de är en stor del av utvecklingen av garaget. Projektet följde en industridesign process med tre faser, analysfas, kreativ fas och en presentationsfas.

Myocardial injury is the leading cause of death in Europe following non-cardiac surgery. Its causes, diagnosis, and treatment are still under investigation by the scientific community. Some research groups have hypothesized a connection between myocardial injury and hypotension during surgery. This thesis investigated the development of a machine learning binary classifier to make a diagnosis of myocardial injury from a set of patients undergoing non-cardiac surgery. Furthermore, it aimed to clarify the potential of hypotension as a predictor of the pathology. After an evaluation of the requirements that the model had to meet, it was decided to use a decision tree. 4 features were selected combining ANOVA and the domain knowledge of the doctors. The classifier obtained a F1 macro-score of 0.68, showing to have potential in classifying patients as positive or negative. Among the selected features, hypotension obtained the lowest predictive power. Despite the performance of the model, further research is needed to validate the results across different populations and to investigate the use of hypotension as a predictor of myocardial injury. / Myokardiell skada är den främsta dödsorsaken i Europa efter icke-kardiell kirurgi. Dess orsaker, diagnos och behandling är fortfarande under utredning av vetenskapssamfundet. Vissa forskargrupper har hypotiserat om ett samband mellan myokardiell skada och hypotoni under operationen. Denna avhandling undersökte utvecklingen av en binär klassificerare med maskininlärning för att ställa diagnos på myokardiell skada hos en grupp patienter som genomgick icke-kardiell kirurgi. Dessutom syftade den till att klargöra hypotoniens potentiella betydelse som en prediktor för sjukdomen. Efter en utvärdering av modellens krav beslutades det att använda ett beslutsträd. Fyra egenskaper valdes ut genom att kombinera ANOVA och läkarnas domänkunskap. Klassificeraren uppnådde en F1-makropoäng på 0,68 och visade potential att klassificera patienter som positiva eller negativa. Bland de valda egenskaperna hade hypotension den lägsta prediktiva förmågan. Trots modellens prestanda behövs ytterligare forskning för att validera resultaten i olika populationer och undersöka användningen av hypotension som en prediktor för myokardiell skada.