Com o objetivo de se caracterizar o processo de recombinação somática em Trichoderma pseudokoningii foram feitos cruzamentos via anastomose de hifas entre duas linhagens contrastantes para quatro marcadores de auxotrofia, coloração dos conídios e marcadores de RAPD. Foram feitos quatro cruzamentos, sendo analisados um total de 1052 colônias obtidas a partir de suspensões de conídios provenientes das colônias heterocarióticas. Sessenta e oito colônias recombinantes foram analisadas quanto às marcas de auxotrofia em quatro gerações de crescimento, sendo observado que 58 mantiveram o fenótipo recombinante, enquanto que as colônias restantes reverteram para um dos parentais. A maioria das colônias recombinantes se mostrou instável. Entretanto, após 4 gerações de crescimento estas colônias se tornaram estáveis para as marcas de auxotrofia avaliadas. As colônias recombinantes instáveis apresentaram bordas de crescimento irregular, esporulação esparsa e a freqüente formação de setores. Estas colônias recombinantes foram analisadas quanto aos marcadores RAPD, tendo mostrado grande similaridade, em relação ao perfil de bandas apresentado, com a maioria dos primers analisados. Somente com um primer foi possível visualizar a presença de uma banda polimórfica entre os recombinantes e a presença de bandas nos parentais não existentes em alguns recombinantes. Cinco colônias recombinantes foram analisadas quanto ao perfil de bandas cromossomais (PFGE), tendo sido observado que 2 colônias apresentaram padrões cromossomais igual a um dos parentais e 3 colônias apresentaram padrões recombinantes. Nos estudos citológicos verificou-se a formação de conídios uninucleados na conidiogênese, e a presença de conídios verdes maduros multinucleados, devido a prováveis divisões nucleares durante o processo de maturação dos conídios. Observou-se durante a formação dos heterocários a ocorrência de anastomoses e a passagem de núcleos, tendo sido observado a presença de núcleos com várias conformações, sugerindo um movimento ativo dos mesmos. Os resultados acima sugerem a ocorrência de mecanismos de recombinação no heterocário (recombinação somática), diferentes daqueles descritos para o ciclo parassexual ou parameiose, sendo proposto a ocorrência da degradação, no heterocário, dos núcleos de um dos parentais envolvidos nos cruzamentos (parental não prevalente) e a incorporação de segmentos destes em núcleos íntegros do parental prevalente. Se estes eventos realmente estiverem ocorrendo, sugere-se que estes sejam devido a possíveis reações limitadas de incompatibilidade vegetativa, ocasionando processos de lise e morte celular em algumas regiões do micélio heterocariótico. / To understand the somatic recombination process in Trichoderma pseudokoningii, auxotrophic complementary mutant strains were used to produce 4 heterokaryons. These strains were contrasting for four auxotrophic markers, conidia colors and for some RAPD markers. It was analyzed a total of 1052 colonies obtained from conidial suspensions of the heterokaryotic colonies. Stability of auxotrophic markers was evaluated in 68 recombinant colonies after four growing generations. In this analysis, 58 colonies kept the recombinant phenotype, while 10 reverted to one parental strain. Most of the recombinant colonies were initially unstable, but after at least 4 growing generations these recombinants became stable for auxotrophic markers. The unstable recombinant colonies showed irregular growing borders, sparse sporulation and frequent sector formation. The recombinant colonies were analyzed by RAPD technique. These colonies showed high similarity for the most of used primers. However, one primer showed a polymorphic band and some recombinants missing bands observed in parental strains. Chromosomal band profile of 5 recombinants and two parental strains were analyzed by Pulsed Field Gel Electrophoresis technique (PFGE). Two recombinants showed parental profiles and 3 showed recombinant profiles, respectively. In cytological studies of the conidiogenesis was observed the formation of only uninucleated conidia. However, presence of multinucleated mature green conidia was evident, probably due to nuclear divisions in course of maturing process of the conidia. During the process of heterokaryotic mycelium formation was possible to observe the occurrence of anastomosis that showed nuclear transfer. The presence of nuclei in several conformations was observed at the different regions of the heterokaryon, suggesting an active movement. The results presented in this study suggest the occurrence of recombination mechanisms in the heterokayon (somatic recombination), different from those described in classic parasexual cycle or parameiosis. Thus, it was proposed that may occur during this recombinant process the degradation of nuclei from one parental (non-prevalent parental) in the heterokaryon, and that the resulting chromosomal fragments may be incorporated into whole nuclei of the another parental (prevalent parental). If this natural transformation is occurring during this recombination process could be suggested that this event is due to a limited incompatible vegetative reactions, generating cellular lyses and death in some regions of the heterokaryotic mycelium.

Abstract Most plants grow in association with arbuscular mycorrhizal (AM) fungi in their roots forming the so-called AM symbiosis. AM symbiosis is usually beneficial to the host as it improves plant survival and performance. However, AM symbiosis also entails a cost to the plant in terms of the carbon allocated to the fungus. In sexually dimorphic plants, more than one type of individual can be recognised with regard to their sexual expression or gender. The cost of reproduction in these plants will differ in relation to the relative investment in male versus female function, as the female and the male sexual functions incur different costs. This different cost of reproduction may be translated into differences in other plant functions between the sexes as all functions are connected through trade-offs. Therefore, since sexes differ in resource needs and allocation patterns, and AM mediate resource acquisition and allocation patterns through imposing both costs and benefits to the plant, the sexes of dimorphic plant species may possess, at least theoretically, a different relationship with their AM roots symbionts. In this thesis, I have investigated whether the sexes in sexually dimorphic plant species differ in their mycorrhizal relationship, and if so, in which ways. Several plant life history traits were studied in the dioecious species Antennaria dioica and also in the gynodioecious Geranium sylvaticum using greenhouse, common-garden and field experiments. Resource acquisition, resource allocation, and both plant and fungal benefits from AM symbiosis were considered. Mainly beneficial effects of AM symbiosis were observed in both sexes of the two dimorphic plant species for most of the studied plant life history traits. Overall, both partners benefited from the AM association. However, several sex-specific benefits were detected which were not uniformly present in all experiments for any given trait. Moreover, the responses observed in certain life history traits were dependent on both the AM fungal and plant species involved in the symbiosis. Remarkably, plants gained sex-specific benefits from the same species of AM fungi and the fungal benefit differed depending on the sex of the host plant. In addition, mycorrhizal benefits were lost under certain environmental conditions. To summarise, the results obtained in this study highlight the complexity of AM interactions. My results suggest that the plant-mycorrhizal fungus relationship may differ depending on the sex of the host plant. Through sex-specific effects on survival, growth and reproduction of the hosts, AM fungi may play a role in the evolution of the life histories in the studied species. In addition, sex-specific relationships between plants and their mycorrhizal symbionts may have potential important consequences for the population dynamics of the sexual morphs and the coevolution of the mycorrhizal relationship.

En raison des activités anthropiques croissantes, de larges sites sont contaminés par les métaux lourds qui affectent les systèmes biologiques. La souche Oidiodendron maius Zn pourrait être un organisme intéressant dans un programme de bioremédiation étant à la fois un champignon mycorhizien éricoïde et une souche tolérante aux métaux lourds. Pour comprendre les mécanismes de la tolérance de cette souche, trois approches différentes ont été menées.La première approche a abouti à la génération de mutants du gène superoxyde dismutase 1 (OmSOD1). Il s'agit de la première délétion d'un gène par recombinaison homologue chez un champignon mycorhizien. Nous démontrons que l'absence d'OmSOD1 cause un déséquilibre dans l'homéostasie rédox et un changement dans le dialogue entre le champignon et sa plante hôte.La deuxième approche a été basée sur la complémentation fonctionnelle d'un mutant de levure en utilisant une banque d'ADNc d'O. maius. Nous décrivons les premiers transporteurs d'un champignon mycorhizien éricoïde capables de conférer la tolérance au Zn dans des levures. Deux gènes ont été isolés et nommés OmCDF et OmFET. L'expression hétérologue de ces deux gènes dans différents mutants de levure a permis de conférer la tolérance au Zn. De plus, OmCDF a également permis de conférer la tolérance au Co. Nos données suggérent que OmCDF est un transporteur de Zn responsable du transfert du Zn cytoplasmique vers le réticulum endoplasmique, tandis que l'expression d'OmFET pourrait neutraliser la toxicité engendrée par le Zn en augmentant le contenu du Fe dans la cellule. La troisième approche a concerné le criblage d'une collection de mutants aléatoires d'O. maius sur Zn, Cd et ménadione. Nous décrivons la caractérisation d'un mutant dans le gène nmr. Dans ce mutant, une diminution de la teneur en glutamine et asparagine, ainsi qu'une réduction de l'activité de la glutamine synthétase ont été enregistrées. Les liens possibles entre la tolérance au stress oxydatif et le métabolisme azoté sont discutés. / Due to increasing anthropogenic activities, large areas are highly contaminated by heavy metals which are affecting biological systems. Oidiodendron maius strain Zn could be an interesting organism in a bioremediation program being both an ericoid mycorrhizal fungus and a heavy metal-tolerant strain. To understand the mechanisms underlying the oxidative stress tolerance of this strain, three different approaches were used. The first approach allowed us to obtain superoxide dismutase 1 (SOD1) null mutants. The most important technical advance in this work was the first successful disruption of a gene by homologous recombination in a mycorrhizal fungus. We demonstrate that the lack of OmSOD may cause an imbalance in the redox homeostasis and an alteration in the delicate dialogue between the fungus and its host plant. The second approach was based on a yeast functional complementation screening using an O. maius cDNA library. In this work we report the first transporters of an ericoid mycorrhizal fungus capable of conferring Zn tolerance to yeast transformants. Two full-length cDNAs were isolated and named OmCDF and OmFET. The heterologous expression of these two genes in various yeast mutants conferred resistance to zinc. Additionally, OmCDF expression also conferred Co tolerance. We provide evidence that OmCDF functions as a Zn transporter responsible for relocating cytoplasmic Zn into the endoplasmic reticulum, whereas expression of OmFET could counteract Zn toxicity by increasing Fe content of cells. The third approach consisted in the screening of a collection of O. maius random-mutants on Zn, Cd and menadione. We report the characterization of an O. maius-mutant that carries a mutation in the nmr gene. In this mutant, a decrease of glutamine and asparagine pools, and a reduction of the activity of glutamine synthase were recorded. Possible links between the oxidative stress tolerance and the nitrogen metabolism are discussed.

This thesis investigates the effects of co-culturing microorganisms including 37 yeast, 38 bacteria, nine diazotrophic cyanobacteria, and three fungi on biomass and lipid production in fresh- and saltwater algae. Algal lipid content was measured using Nile Red method and gravimetric techniques. Among the algal strains tested, freshwater Coelastrum sp. 46-4, and saltwater Cricosphaera sp. 146-2-9, showed enhanced biomass yield and lipid content in response to co-culture with bacteria, cyanobacteria, and fungi. While co-culture with yeast caused inhibition of algal productivity, no difference in algal productivity was observed between nitrogen-free diazotrophic cyanobacterial co-culture and nitrogen-replete monoalgal culture. Results indicated that extracellular compounds from the freshwater bacteria Pseudomonas stutzeri and marine fungus Fusarium sp. significantly account for stimulation of lipid accumulation within algal cells, while co-cultivation with live microorganism cells stimulated biomass production in algae.

Among fungal pathogens responsible for opportunistic infections, species of the genus Candida have a major role (Mitchell, 1998). Various Candida species cause superficial infections which can be cured by the currently available antifungal arsenal (Noble and Johnson, 2007). However, species of the genus Candida are also responsible for life-threatening systemic infections, particularly in immunocompromised patients with weakened immune system. Among Candida species, C. albicans, which can also be a commensal of the skin and the gastrointestinal and genitourinary tracts, is responsible for the majority of Candida bloodstream infections. However, there is an increasing incidence of infections caused by C. glabrata because it is less susceptible to azoles. Other medically important Candida species include C. parapsilosis, C. tropicalis and C. dubliniensis. The problem has been further worsened by the emergence of many drug resistant isolates which pose a major hurdle during a given treatment regimen. Therefore, there is a dire need to identify novel drug targets and the current study focuses on one such protein found in C. albicans and related Candida species. CaGAL102 does not encode a functional galactose epimerase CaGAL102 was previously identified in the lab as a paralog of CaGAL10. CaGAL10 endoes a functional UDP-galactose 4-epimerase and it can complement a Scgal10 null strain. Further, work on the Gal10 protein in the encapsulated yeast Cryptococcus neoformans identified two Gal10 paralogs in the genome, Uge1 and Uge2 with distinct functions (Moyrand et al., 2008). A similar scenario is found in S. pombe in which two Gal10 sequence homologs have been annotated. In the light of these observations, we wanted to test if CaGAL102 also encodes a functional ScGAL10 homolog. We found that CaGAL102 could not complement Scgal10 null strain though there was a strong conservation in the cofactor and the catalytic motif in both the proteins. We found after a careful literature review that Gal10 belongs to a family of proteins called the short chain dehydratase/reductase family (SDR) (Jornvall et al., 1995), members of which are characterised by the presence of glycine rich cofactor binding motif at the N-terminus and an YXXXK catalytic motif. Proteins belonging to the SDR family have a residue level identity of 15-30% indicating early duplication and divergence. Based on our literature survey we carried out a BLAST search in the NCBI protein database using CaGal102 as the bait protein. We found that CaGal102 is 32% identical at the protein level to dTDP-glucose 4,6 dehydratase (RmlB), another member of the SDR family. RmlB is the second enzyme of the rhamnose biosynthetic pathway which gives rise to dTDP-rhamnose. This pathway is involved in cell wall biosynthesis in bacteria and it has been shown that rmlB is essential for growth of Mycobacterium smegmatis (Li et al, 2006). Interestingly rhamnose is not present in the cell wall of C. albicans. Biochemical characterisation of CaCaGal102 A plant homolog of RmlB is found in A. thaliana which uses UDP-glucose as the substrate (Oka et al., 2007). Based on our alignment data we identified many critical residues in CaGal102. Most importantly we identified that lysine at position 159 lies in the YXXXK motif and could be important for activity. We therefore, mutated the lysine at position 159 to alanine. In order to find out the biochemical function of CaGal102 in vitro, we cloned expressed and purified recombinant wild type and catalytic mutant proteins from E. coli and used the purified proteins for our assays. We found that CaGal102 uses UDP-glucose as the preferred substrate. To further substantiate our data, we reintegrated the wild type or the mutant alleles in the native locus of CaGAL102 and checked for the rescue of morphology defects like filamentation and sensitivity to cell wall damaging agents. We also found that the Cagal102∆/∆ strain is avirulent in a mouse model of systemic infection. We have also carried out infection studies with the null mutant and the wild type and the catalytic mutant reintegrant strains. Our observation suggests that reintegrating one copy of the wild type allele rescues the virulence defect. Interestingly the strain harbouring one copy of the mutant allele behaves like the null mutant in a mouse model of systemic infection. We have also identified sequence homologs of CaGal102 in related Candida species. It is plausible to think that the homologs in related species also have similar effects and hence targeting this protein by a small molecule could help in treating candidiasis caused by related species. CaGAL102 is involved in cell wall architecture in C. albicans To elucidate the role of CaGal102 in C. albicans we generated a knockout out strain and studied various mutant phenotypes. The most striking observation was that the cells of the null mutant were filamentous as compared to the wild type control when grown in normal rich media. Further the cells were sensitive to various cell wall damaging agents and also to hygromycin B. We reasoned that lack of CaGal102 causes perturbation in the cell wall architecture rendering the cells sensitive to various cell wall damaging agents. To further strengthen this hypothesis, we decided to study the genetic interaction of CaGAL102 with genes known to be involved in cell wall biosynthesis in C. albicans. One of the candidate genes we chose for our study was GAL10, deletion of which in C. albicans renders the cells sensitive to various cell wall damaging agents. Loss of function of UGE1 in C. neoformans impaired biosynthesis of a cell wall component, galactoxylomannan. We found that cells lacking both Gal102 and Gal10 adhered to nylon membranes poorly as compared to single mutants or the wild type control. The second gene we chose was a P-type ATPase, PMR1 deletion of which causes increased sensitivity to cell wall damaging agents and hyper-activation of the cell wall integrity pathway similar to Cagal102∆/∆ strain. We found that cells lacking both Pmr1 and Gal102 were more sensitive to hygromycin B as compared to the single mutants. This confirmed our idea that CaGal102 is a novel gene involved in cell wall biogenesis in C. albicans. REFERENCES: Mitchell, A.P. (1998) Dimorphism and virulence in Candida albicans. Curr Opin Microbiol, 1, 687-692. Noble, S.M. and Johnson, A.D. (2007) Genetics of Candida albicans, a diploid human fungal pathogen. Annu Rev Genet, 41, 193-211. Moyrand, F., Lafontaine, I., Fontaine, T. and Janbon, G. (2008) UGE1 and UGE2 regulate the UDP-glucose/UDP-galactose equilibrium in Cryptococcus neoformans. Eukaryot Cell, 7: 2069-2077. Jornvall Hans, Persson Bengt, Krook Maria,‟ Atrian Silvia, Gonzalez-Duarte Roser, Jeffery Jonathan, and Ghosh Debashis (1995). Short-Chain Dehydrogenases Reductases (SDR). Biochemistry, 34: 6004-13. Li, W., Xin, Y., McNeil, M.R. and Ma, Y. (2006) rmlB and rmlC genes are essential for growth of mycobacteria. Biochem Biophys Res Commun, 342: 170-178. Oka, T., Nemoto, T. and Jigami, Y. (2007) Functional analysis of Arabidopsis thaliana RHM2/MUM4, a multidomain protein involved in UDP-D-glucose to UDP-L-rhamnose conversion. J Biol Chem, 282: 5389-5403.

A micologia marinha é uma ciência relativamente recente e pouco se conhece sobre a diversidade das suas comunidades. Assim, o isolamento, triagem e preservação de fungos derivados do mar podem levar à descoberta de novas tecnologias. O objetivo deste estudo foi conhecer a diversidade de fungos filamentosos derivados marinhos e selecionar isolados capazes de produzir enzimas celulolíticas. Para tanto, foram isolados seletivamente fungos filamentosos a partir de amostras de macro-organismos marinhos coletados em 2007 e 2008. Os resultados demonstraram uma ampla diversidade de fungos potencialmente celulolíticos, pertencentes ao filo Basidiomycota e Ascomycota. Nos experimentos de produção de celulases, 17 apresentaram resultados satisfatórios de CMCase e FPase e foram selecionados para a avaliação da Celobiase. Os experimentos de cinética enzimática apresentaram os melhores resultados de produção de celulases em meio contendo farelo de trigo. O trabalho demonstra o potencial para aplicação biotecnológica dos fungos e estimula novos estudos com as celulases. / The Marine mycology is a relatively recent and little is known about the diversity of its communities. Thus, the isolation, separation and preservation of fungi derived from the sea can lead to the discovery of new technologies. The aim of this study was the diversity of filamentous fungi isolates derived marine and select capable of producing cellulolytic enzymes. It had been selectively isolated filamentous fungi from samples of marine macro-organisms collected in 2007 and 2008. The results showed a wide range of potential cellulolytic fungi, belonging to the phylum Basidiomycota and Ascomycota. In the experiments to produce cellulases, 17 had satisfactory results of CMCase and FPase and were selected for evaluation of cellobiase. The enzyme kinetics experiments showed better results for the production of cellulases in a medium containing wheat bran. The work demonstrates the potential for biotechnological application of fungi and stimulate further research with cellulases.

Die Rote Liste informiert über die Gefährdungssituation der Arten und Lebensräume und stellt eine Grundlage für die Fachplanung im Naturschutz dar. In der Broschüre werden 5.360 in Sachsen vorkommende Pilzarten aufgelistet und bewertet. Eine Rote Liste für Pilze in Sachsen erschien zuletzt 1999.

Ciidae is a fairly homogenous family of mycetobiont, mycetophagous beetles (Coleoptera, infraorder Cucujiformia), with a worldwide distribution and about 750 described species. They have a body length of 0.5‒7 mm, a mostly uniform colouration from light brown to almost black, and usually a fairly cylindrical body shape. The knowledge on the taxonomy, morphology, phylogenetic relationships, geographic distribution (faunistics), and ecology of Ciidae is quite fragmentary. Part I of the thesis provides the first detailed study of head morphology (by scanning electron microscopy) in a Ciidae species: Cis chinensis. Many new structural elements are detected and named to ease their use in more sophisticated forthcoming taxonomic work. Most head characters are compared with Ciidae from genera Ennearthron, Octotemnus, Ropalodontus, and Xylographus, with a focus on the area between compound eye and buccal cavity. This revealed a great uniformity, with a near-identical structuring of subantennal groove, margin of buccal cavity, anterior tentorial pit, and first antennomere (scapus). A comparison with Tenebrio molitor from the closely related Tenebrionidae (data from the literature) revealed great similarity, but also differences in some characters, such as the absence of a subantennal groove. Part II presents the first study of the Ciidae fauna of the Caspian (or Hyrcanian) Forest of northern Iran, a region known to have conserved much pre-Pleistocene fauna and flora. In 2010‒2018, Ciidae were sampled in 62 localities across the Iranian provinces Gilan, Mazandaran, and Golistan, from below sea level on the Caspian Sea southern coast up to near 2000 m a.s.l. on the northern slopes of the Alborz Mountains. 19 Ciidae species were identified in the basidiomes of 31 species of bracket fungi (Basidiomycetes: mainly Polyporales, Hymenochaetales, and Russulales-Stereaceae). For all Ciidae species taxonomically relevant morphological features are described and illustrated; the distribution over collecting localities and occurrence in host fungus species are given. An identification key for the species of the area is given. The identification of species is discussed, as well as some morphological characters and their systematic implications, the distribution and ecology of the individual species (especially the pest species Cis chinensis), the evidence from overall patterns with regard to geographic and elevational distribution and fungus host ranges, and evidence on possible faunal links. No previously undescribed Ciidae species was found, but most species are new for Iran. Illustrations are provided for morphological characters, distribution in the area (maps), and coexistence of species (column diagrams).:Abstract 7 Kurzfassung 7 Key words 8 List of figures and tables 9 List of abbreviations 13 Introduction to Ciidae 16 Taxonomy and systematics of Ciidae 16 Ecology and faunistics of Ciidae 19 Part I: External Head Morphology of Cis chinensis 22 1. Introduction to head morphology of Ciidae 22 2. Material and methods 22 2.1. Specimens 22 2.2. Pictorial documentation 23 2.3. Terminology 23 2.4. Morphological directions 23 2.5. Abbreviations 24 3. Description of head of Cis chinensis 24 3.1. Sexes and male morphs 24 3.2. Head capsule 24 3.3. Clypeus, labrum and epipharynx 26 3.4. Mandibles 27 3.5. Maxillae 28 3.6. Labium and hypopharynx 29 3.7. Antennae 30 4. Discussion of head morphology 32 4.1. Notes on morphological terms and interpretations 32 4.2. Comparison with some other Ciidae 35 4.3. Comparison with Tenebrio molitor (Tenebrionidae) 37 Part II: Ciidae in Caspian-Hyrcanian Forest in Northern Iran 50 1. Introduction to Caspian-Hyrcanian forest 50 1.1. Geography of the Caspian Forest 50 1.2. History and significance of the Caspian Forest 51 1.3. Climate of the Caspian Forest 51 1.4. Geographic relationships of the Caspian Forest 51 1.5. Trees of the Caspian Forest 52 1.6. Fungi of the Caspian Forest 52 1.7. Ciidae of the Caspian Forest 53 2. Material and methods 53 2.1. Area covered and collecting localities 53 2.2. Collecting of Ciidae 54 2.3. Morphological preparation and pictorial documentation 54 2.4. Processing of images 54 2.5. Identification of fungus species 55 2.6. Identification of Ciidae species 55 2.7. Morphological data and terminologies 56 2.8. Abbreviations 56 3. Results on Ciidae in Caspian Forest 56 3.1. Survey of observed Ciidae species and their systematics 56 3.2. Survey of observed host fungus species and their systematics 59 3.3. Morphology and distinguishing characters of Ciidae 60 3.4. Results on Cis chinensis 69 3.5. Results on Cis submicans 71 3.6. Results on Cis comptus 73 3.7. Results on Cis striatulus 75 3.8. Results on Cis tomentosus 77 3.9. Results on Cis reitteri 79 3.10. Results on Cis castaneus 81 3.11. Results on Cis lugowoji 83 3.12. Results on Cis fissicollis 85 3.13. Results on Cis festivus 87 3.14. Results on Ennearthron cornutum 88 3.15. Results on Orthocis reflexicollis 90 3.16. Results on Strigocis bicornis 92 3.17. Results on Sulcacis fronticornis 94 3.18. Results on Sulcacis nitidus 96 3.19. Results on Ropalodontus baudueri 97 3.20. Results on Ropalodontus perrini 99 3.21. Results on Octotemnus rugosopunctatus 101 3.22. Results on Xylographus bostrichoides 104 3.23. Identification key to Ciidae of Caspian Forest 106 4. Discussion of Ciidae in Caspian Forest 109 4.1. Taxonomic distinctions 109 4.1.1. Cis multidentatus species group: Cis chinensis 109 4.1.2. Cis boleti species group: Cis submicans 110 4.1.3. Cis comptus species group: Cis comptus and Cis striatulus 110 4.1.4. Cis punctulatus species group: Cis tomentosus and Cis reitteri 111 4.1.5. Cis castaneus species group: Cis castaneus and Cis lugowoji 112 4.1.6. Cis fissicollis (not assigned to a species group) 113 4.1.7. Cis festivus species group: Cis festivus 113 4.1.8. Genus Ennearthron: Ennearthron cornutum 114 4.1.9. Genus Orthocis: Orthocis reflexicollis 114 4.1.10. Genus Strigocis: Strigocis bicornis 114 4.1.11. Genus Sulcacis: Sulcacis fronticornis and S. nitidus 115 4.1.12. Genus Ropalodontus: Ropalodontus baudueri and R. perrini 115 4.1.13. Genus Octotemnus: Octotemnus rugosopunctatus 115 4.1.14. Genus Xylographus: Xylographus bostrichoides 116 4.2. Morphological characters and systematic implications 116 4.2.1. Sensillifers of antennal club 116 4.2.2. Fovea on 1st abdominal ventrite 116 4.2.3. Cephalofoveae on forehead 117 4.3. Distribution and ecology of individual species 117 4.3.1. Distribution and ecology of Cis chinensis 118 4.3.2. Distribution and ecology of Cis submicans 122 4.3.3. Distribution and ecology of Cis comptus 122 4.3.4. Distribution and ecology of Cis striatulus 123 4.3.5. Distribution and ecology of Cis tomentosus 123 4.3.6. Distribution and ecology of Cis reitteri 124 4.3.7. Distribution and ecology of Cis castaneus 124 4.3.8. Distribution and ecology of Cis lugowoji 124 4.3.9. Distribution and ecology of Cis fissicollis 125 4.3.10. Distribution and ecology of Cis festivus 125 4.3.11. Distribution and ecology of Ennearthron cornutum 125 4.3.12. Distribution and ecology of Orthocis reflexicollis 126 4.3.13. Distribution and ecology of Strigocis bicornis 126 4.3.14. Distribution and ecology of Sulcacis fronticornis 127 4.3.15. Distribution and ecology of Sulcacis nitidus 127 4.3.16. Distribution and ecology of Ropalodontus baudueri 128 4.3.17. Distribution and ecology of Ropalodontus perrini 128 4.3.18. Distribution and ecology of Octotemnus rugosopunctatus 129 4.3.19. Distribution and ecology of Xylographus bostrichoides 129 4.4. Overview of distribution ranges 130 4.5. Overview of elevation ranges 131 4.6. Overview of fungus host ranges 132 4.7. Ciidae fauna of the wider region 136 4.8. Faunal links and dispersal of Ciidae of Caspian Forest 137 Acknowledgements 250 References 251 Appendix: Collecting data, host fungi, co-occurrence 257

No description available.

<p><strong>Apstrakt je obrađen tehnologijama za optičko prepoznavanje teksta (OCR).</strong></p><p>Ispitana je antioksidativna aktivnost različito pripremljenih napitaka i fermentativnih tečnosti od čajne gljive, i to prvenstveno praćenjem sposobnosti transformacije i stabilizacije reaktivnih hidroksi-radikala i redukcije stabilnih 1,1-difeniI- 2-pikriIhidraziI (DPPH) radikala. Određeni su i neki od metabolita kombuhe koji deluju kao antioksidanti (vitamini B₂ i C), kao i organske kiseline koje stabilizuju napitak od čajne gljive. Osnovne analitičke tehnike kori&scaron;ćene u radu bile su ESR, HPLC, TLC, spektrofotometrija proizvoda enzimskih reakcija i volumetrija.</p> / <p><strong>Abstract was processed by technology for Optical character recognition (OCR).</strong></p><p>The antioxidant activity of differently prepared beverages and fermentative liquids of tea fungus was examined, primarily by following of ability for transformation and stabilization of reactive hydroxyl-radicals and reduction of stable 1,1- diphenyl-2-picrylhydrazyl (DPPH) radicals. Some of the metabolites of kombucha that act as antioxidants (vitamins B2 and C) and organic acids that stabilize tea fungus beverage were determined. The primary used analytical techniques were ESR, HPLC, TLC, spectrophotometry of products of enzymatic reactions and voIumetry.</p><p>&nbsp;</p>