Fingers should demonstrate my filmmaking ability and encourage future employers to hire me to direct a project. This paper will explore in details what methods were used during the production with focus on working within the means of the budget. In the following chapters, I will discuss Fingers, including the writing, preproduction, directing, cinematography, editing, and the final product.

Este trabalho teve como objetivo observar, analisar e discutir como ocorre a busca por sinais-alvo, em indivíduos surdos, fluentes na Língua Brasileira de Sinais (Libras); verificar se ocorre, nesses indivíduos o fenômeno ponta dos dedos (TOF); e descrever os aspectos fonético-fonológicos na ocorrência do fenômeno. O TOF corresponde ao fenômeno ponta da língua (TOT) nas línguas orais. Esse fenômeno ocorre quando há o esquecimento momentâneo de palavras conhecidas e o sentimento de que essa palavra está prestes a ser recuperada. Tal fenômeno oferece pistas sobre o processamento da linguagem e a recuperação lexical. Poucos trabalhos foram realizados sobre este fenômeno nas línguas de sinais (THOMPSON; EMMOREY; GOLLAN, 2005) e essa investigação é importante, pois, por ser uma língua de modalidade distinta (visual-espacial), possui processos fonológicos particulares. Nesse sentido, foi elaborado um teste para eliciar o fenômeno em 34 adultos surdos, que relataram utilizar a Libras como principal meio de comunicação. O teste consistia na realização do sinal referente a personalidades famosas e a cidades no mundo. Foram exibidas imagens e o participante deveria dizer se sabia ou não o sinal referente à pessoa ou à cidade, ou se estava experienciando o TOF. Neste último caso, o participante deveria sinalizar o que lembrasse do sinal- alvo. Foram realizados 69 estímulos por participantes, somando um total de 2346 estímulos e, como resultado obtivemos a ocorrência de 20 TOFs (0,9% dos estímulos). Em todos os casos de TOF foi recuperado ao menos um dos parâmetros fonético-fonológicos (quais sejam, configuração de mão, localização/ espaço, orientação, movimento, número de mãos). Dos parâmetros fonético-fonológicos recuperados, a configuração de mão foi recuperada em 65% dos casos (13 vezes); a localização em 70% (14 vezes); o espaço em 85% (17 vezes); o movimento em 35% (7 vezes); a orientação em 50% (10 vezes); e o número de mãos em 90% dos casos (18 vezes). Corroboramos a conclusão de Thompson, Emmorey e Gollan (2005) de que o movimento é o parâmetro menos recuperado no momento do TOF. Esse fato pode indicar que os parâmetros localização, configuração de mão e orientação (mais recuperados no momento do TOF) constituem o onset da estrutura silábica do sinal, isto é, o segmento inicial do sinal. Além disso, a ocorrência do fenômeno ponta dos dedos na Libras ajuda a confirmar separação, no processamento da recuperação lexical em codificação semântica e codificação fonológica estabelecidas em modelos de recuperação de línguas orais (DELL, 1986; GARRETT, 1988; LEVELT, 1989). / The aim of this research is to analyze and discuss how the search for target signs occurs in the lexical retrieval in deaf people using Brazilian Sign Language (Libras); to verify if the \"tip of the fingers\" (TOF) phenomenon occurs; and describe the phonological aspects in the occurrence of the phenomenon. The TOF phenomenon mirrors the \"tip of the tongue\" phenomenon in oral languages. This phenomenon occurs when there is momentary forgetfulness of known words and the feeling that this word is about to be recovered. This phenomenon offers clues about language processing and lexical retrieval. Few studies have been carried out on this phenomenon in sign languages (THOMPSON; EMMOREY; GOLLAN, 2005) and this research is important because sign language has a different modality and therefore it has particular phonological processes. In this sense, a test was prepared to elicit the phenomenon in 34 deaf adults, who reported using Libras as the main means of communication. The test consisted of the signing regarding proper names of famous personalities and cities in the world. Images were displayed and the participant should say whether or not he knew the sign for the person or city, or whether he was experiencing TOF. In the latter case, the participant should sign what he remembered of the target signal. A total of 69 stimuli were performed per participant, totaling 2346 stimuli and, as a result, we obtained the occurrence of 20 TOFs (0.9% of the stimuli). In all TOF cases, at least one of the phonological parameters (ie, hand configuration, location / space, orientation, movement, number of hands) was retrieved. Of those, the hand configuration was recovered in 65% of the cases (13 times); the location in 70% (14 times); space 85% (17 times); movement in 35% (7 times); orientation by 50% (10 times); and the number of hands in 90% of the cases (18 times). We corroborate the conclusion of Thompson, Emmorey and Gollan (2005) that movement is the least recovered parameter at the time of TOF. This fact may indicate that the parameters localization, hand configuration and orientation (more retrieved while in TOF state) constitute the onset of the syllabic structure of the sign. In addition, the occurrence of the \"tip of the fingers\" phenomenon in Libras helps to confirm separation, in the processing of lexical retrieval in semantic encoding and phonological encoding established in lexical retrieval models of oral language (GARRETT, 1988; LEVELT, 1989).

<div>Manual function is a key determinant of functional independence. It is well known that manual dexterity declines with aging and negatively impacts quality of life. Therefore, much work has focused on understanding the biomechanics and motor control of manual function in general, and the action of the fingers in particular. Previous research has revealed consistent patterns of interdependence in the action of the fingers that (1) alter with age, and (2) have consequences for manual control, and thereby manual function. Most of this previous work on finger behavior quantifies finger capacities and interactions in terms of maximal forces. However, activities of daily living likely require individuals to rapidly change forces more frequently than produce maximal forces. Therefore, the present work quantifies, for the first time, finger capacities and interactions during rapid increase and decrease in finger forces, and how these quantities change with age. </div><div><br></div><div>Young and older adults performed maximal force production tasks and also tasks that required them to rapidly increase or decrease finger forces from three initial force levels using multiple combinations of the fingers of their dominant hand. The maximal finger forces and force rates, and the interdependence of the fingers (enslaving, individuation, sharing, and deficit) during both behaviors are reported in detail. Overall, similarities in finger behavior patterns obtained from maximal force and maximal force rates were observed. However, some differences are also noted, and novel findings (especially, comparison between force increase and decrease) are reported. Finally, future work that may lead to clinical applications is discussed. </div>

Este trabalho teve como objetivo observar, analisar e discutir como ocorre a busca por sinais-alvo, em indivíduos surdos, fluentes na Língua Brasileira de Sinais (Libras); verificar se ocorre, nesses indivíduos o fenômeno ponta dos dedos (TOF); e descrever os aspectos fonético-fonológicos na ocorrência do fenômeno. O TOF corresponde ao fenômeno ponta da língua (TOT) nas línguas orais. Esse fenômeno ocorre quando há o esquecimento momentâneo de palavras conhecidas e o sentimento de que essa palavra está prestes a ser recuperada. Tal fenômeno oferece pistas sobre o processamento da linguagem e a recuperação lexical. Poucos trabalhos foram realizados sobre este fenômeno nas línguas de sinais (THOMPSON; EMMOREY; GOLLAN, 2005) e essa investigação é importante, pois, por ser uma língua de modalidade distinta (visual-espacial), possui processos fonológicos particulares. Nesse sentido, foi elaborado um teste para eliciar o fenômeno em 34 adultos surdos, que relataram utilizar a Libras como principal meio de comunicação. O teste consistia na realização do sinal referente a personalidades famosas e a cidades no mundo. Foram exibidas imagens e o participante deveria dizer se sabia ou não o sinal referente à pessoa ou à cidade, ou se estava experienciando o TOF. Neste último caso, o participante deveria sinalizar o que lembrasse do sinal- alvo. Foram realizados 69 estímulos por participantes, somando um total de 2346 estímulos e, como resultado obtivemos a ocorrência de 20 TOFs (0,9% dos estímulos). Em todos os casos de TOF foi recuperado ao menos um dos parâmetros fonético-fonológicos (quais sejam, configuração de mão, localização/ espaço, orientação, movimento, número de mãos). Dos parâmetros fonético-fonológicos recuperados, a configuração de mão foi recuperada em 65% dos casos (13 vezes); a localização em 70% (14 vezes); o espaço em 85% (17 vezes); o movimento em 35% (7 vezes); a orientação em 50% (10 vezes); e o número de mãos em 90% dos casos (18 vezes). Corroboramos a conclusão de Thompson, Emmorey e Gollan (2005) de que o movimento é o parâmetro menos recuperado no momento do TOF. Esse fato pode indicar que os parâmetros localização, configuração de mão e orientação (mais recuperados no momento do TOF) constituem o onset da estrutura silábica do sinal, isto é, o segmento inicial do sinal. Além disso, a ocorrência do fenômeno ponta dos dedos na Libras ajuda a confirmar separação, no processamento da recuperação lexical em codificação semântica e codificação fonológica estabelecidas em modelos de recuperação de línguas orais (DELL, 1986; GARRETT, 1988; LEVELT, 1989). / The aim of this research is to analyze and discuss how the search for target signs occurs in the lexical retrieval in deaf people using Brazilian Sign Language (Libras); to verify if the \"tip of the fingers\" (TOF) phenomenon occurs; and describe the phonological aspects in the occurrence of the phenomenon. The TOF phenomenon mirrors the \"tip of the tongue\" phenomenon in oral languages. This phenomenon occurs when there is momentary forgetfulness of known words and the feeling that this word is about to be recovered. This phenomenon offers clues about language processing and lexical retrieval. Few studies have been carried out on this phenomenon in sign languages (THOMPSON; EMMOREY; GOLLAN, 2005) and this research is important because sign language has a different modality and therefore it has particular phonological processes. In this sense, a test was prepared to elicit the phenomenon in 34 deaf adults, who reported using Libras as the main means of communication. The test consisted of the signing regarding proper names of famous personalities and cities in the world. Images were displayed and the participant should say whether or not he knew the sign for the person or city, or whether he was experiencing TOF. In the latter case, the participant should sign what he remembered of the target signal. A total of 69 stimuli were performed per participant, totaling 2346 stimuli and, as a result, we obtained the occurrence of 20 TOFs (0.9% of the stimuli). In all TOF cases, at least one of the phonological parameters (ie, hand configuration, location / space, orientation, movement, number of hands) was retrieved. Of those, the hand configuration was recovered in 65% of the cases (13 times); the location in 70% (14 times); space 85% (17 times); movement in 35% (7 times); orientation by 50% (10 times); and the number of hands in 90% of the cases (18 times). We corroborate the conclusion of Thompson, Emmorey and Gollan (2005) that movement is the least recovered parameter at the time of TOF. This fact may indicate that the parameters localization, hand configuration and orientation (more retrieved while in TOF state) constitute the onset of the syllabic structure of the sign. In addition, the occurrence of the \"tip of the fingers\" phenomenon in Libras helps to confirm separation, in the processing of lexical retrieval in semantic encoding and phonological encoding established in lexical retrieval models of oral language (GARRETT, 1988; LEVELT, 1989).

Thesis (Ph. D.)--University of Washington, 2000. / Vita. Includes bibliographical references (leaves 72-79).

A side effect of generating an immune system for defense against invading pathogens is the potential to develop destructive cells that recognize self-tissues. Typically, through the "education" of developing immune cells, the organism inactivates potentially self-destructive cells, resulting in what is called self-tolerance. I proposed to explore the molecular mechanisms responsible for the induction and maintenance of tolerance. Our lab has developed a model of induced immune tolerance to skin and islet allografts utilizing a donor-specific transfusion of spleen cells and a brief course of anti-CD40L antibody. Because the difficulty in isolation of tolerant T cells from this system is prohibitive to performing large screens on these cells directly, I have chosen to study an in vitro CD4+Th1 cell line, A.E7, which can be made anergic via stimulation through the T cell receptor in the absence of costimulation. I hypothesized that anergized T cells upregulate genes that are responsible for the induction and maintenance of anergy and therefore exhibit a unique RNA expression profile. I have screened anergic cells using Affymetrix GeneChips and identified a small number of genes that are differentially expressed long-term in the anergic population compared to mock-stimulated and productively activated controls. The results have been confirmed by quantitative RT-PCR for each of the candidates. One of the most promising, the zinc-finger transcription factor Egr-2, was verified to be expressed long-term by western blotting, demonstrating perfect correlation between Egr-2 protein expression and the anergic phenotype. Silencing Egr-2 gene expression by siRNA in A.E7 T cells prior to anergy induction rescues the cells from the inability to phosphorylate ERK-1 and ERK-2 and also results in increased proliferation in response to antigen rechallenge. In this study I report that Egr-2 is specifically expressed long-term in anergic cells, protein expression correlates inversely with responsiveness to antigen rechallenge, and that Egr-2 is required for the full induction of anergy in T cell clones.

abstract: Effective tactile sensing in prosthetic and robotic hands is crucial for improving the functionality of such hands and enhancing the user's experience. Thus, improving the range of tactile sensing capabilities is essential for developing versatile artificial hands. Multimodal tactile sensors called BioTacs, which include a hydrophone and a force electrode array, were used to understand how grip force, contact angle, object texture, and slip direction may be encoded in the sensor data. Findings show that slip induced under conditions of high contact angles and grip forces resulted in significant changes in both AC and DC pressure magnitude and rate of change in pressure. Slip induced under conditions of low contact angles and grip forces resulted in significant changes in the rate of change in electrode impedance. Slip in the distal direction of a precision grip caused significant changes in pressure magnitude and rate of change in pressure, while slip in the radial direction of the wrist caused significant changes in the rate of change in electrode impedance. A strong relationship was established between slip direction and the rate of change in ratios of electrode impedance for radial and ulnar slip relative to the wrist. Consequently, establishing multiple thresholds or establishing a multivariate model may be a useful method for detecting and characterizing slip. Detecting slip for low contact angles could be done by monitoring electrode data, while detecting slip for high contact angles could be done by monitoring pressure data. Predicting slip in the distal direction could be done by monitoring pressure data, while predicting slip in the radial and ulnar directions could be done by monitoring electrode data. / Dissertation/Thesis / M.S. Bioengineering 2012

Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2016-09-09T19:01:20Z No. of bitstreams: 2 Dissertação- Bruno Rodrigues de Oliveira - 2016.pdf: 17683570 bytes, checksum: 9b371db8aea2547b14113e6356efda81 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-09-12T14:19:29Z (GMT) No. of bitstreams: 2 Dissertação- Bruno Rodrigues de Oliveira - 2016.pdf: 17683570 bytes, checksum: 9b371db8aea2547b14113e6356efda81 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-09-12T14:19:29Z (GMT). No. of bitstreams: 2 Dissertação- Bruno Rodrigues de Oliveira - 2016.pdf: 17683570 bytes, checksum: 9b371db8aea2547b14113e6356efda81 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-06-18 / In everyday applications, the use of hands is essential in performing many tasks. Several situations such as car acidents, labor or stroke, impair mobility and maneuverability of the hands, forcing the individual to seek rehabilitation methods. This study aims to model and develop a device for rehabilitation of the finger that allows performthemovements of flexion / extension, within the limits of each joint. Evaluating types of fingers rehabilitation devices, they are observed its peculiarities and its main advantages, culminating in proposing an efficient model. Thus, this research presents the development of a more affordable device that meets the needs of patients in order to recover their normal activities. / Emaplicações diárias, a utilização dasmãos é indispensável na realização de grande número de tarefas. Diversas situações, como acidentes automobilísticos, trabalhistas ou vasculares, prejudicam a mobilidade e manuseabilidade das mãos, obrigando o indivíduo a procurar métodos de reabilitação. Este estudo tem como objetivo modelar e desenvolver um dispositivo para reabilitação dos dedos da mão que possibilite realizar os movimentos de flexão/ extensão, respeitando os limites de cada articulação. Avaliando tipos de dispositivos de reabilitação de dedos da mão existentes, são observadas suas particularidades e suas principais vantagens, culminando na proposição de um modelo eficiente. Assim, esta pesquisa apresenta o desenvolvimento de um dispositivo mais acessível que atenda às necessidades dos pacientes, auxiliando na recuperação de suas atividades normais.

Très répandues dans le monde vivant, les protéines à doigt de zinc constituent une super-famille dont les membres possèdent un site à zinc de formule générale [ZnII(Cys)4-X(His)X] (x=0, 1 or 2). Tandis que la majorité de ces sites joue un rôle purement structural, certains présentent une fonction réactive, comme la détection de stress oxydant par exemple. En effet, les sites doigt de zinc de Hsp33 et de RsrA ont été décrits comme des interrupteurs rédox[1,2] : transmettant l'information « stress oxydant » sous forme d'un signal structural, via l'oxydation/réduction des cystéines coordonnées au zinc, détruisant/reformant le domaine doigt de zinc. Cependant, certains aspects de l'étape d'oxydation restent mal compris.Étant donné le grand nombre des protéines à doigt de zinc et leurs rôles clés, il est de tout intérêt d'identifier les facteurs contrôlant leur réactivité afin de comprendre pourquoi certaines espèces réactives de l'oxygène (ERO) sont capable d'oxyder des doigts de zinc in vivo, contrairement à H2O2. Durant ce projet, nous avons décidé de nous focaliser sur deux ERO très puissantes et jouant un rôle important en biologie : l'acide hypochloreux (HOCl) et l'oxygène singulet (1O2). Nous étudierons la réactivité des doigts de zinc vis-à-vis de ces deux oxydant en utilisant des modèles peptidiques, reproduisant parfaitement la structure de doigts de zinc courants. / Widely spread in the living world, zinc finger proteins constitute a large superfamily, with a zinc site of general formula [ZnII(Cys)4-X(His)X] (x=0, 1 or 2) as a common feature. Whereas the majority of such sites plays a purely structural role, a few of them exhibit a reactive function (e.g. oxidative stress detection). Indeed, zinc finger sites of Hsp33 and RsrA have been shown to act as redox switches[1,2]: transmitting the information “oxidative stress” as a structural signal, by means of oxidation/reduction of its ZnII-coordinating cysteines. However, the precise mechanism of the oxidation step remains poorly understood.Given the occurrence of zinc finger proteins and their key roles, it is of high biological interest to identify factors controlling their reactivity and to understand why some ROS are able to oxidize them in vivo, on the contrary to H2O2. In this project, we decided to focus on two major ROS: hypochlorous acid (HOCl), a key player of the immune response, and singlet oxygen (1O2), produced in significant amount by photosynthetic organisms. By use of peptide model complexes, reproducing perfectly the structure of some archetypal zinc fingers, we investigated the reactivity of zinc fingers toward those ROS.

The utility of a model organism for studying biological processes is closely tied to its amenability to genome manipulation. Although tools for targeted genome engineering in mice have been available since 1987, most organisms including zebrafish have lacked efficient reverse genetic tools, which has stymied their broad implementation as a model system to study biological processes. The development of zinc finger nucleases (ZFNs) that can create double-strand breaks at desired sites in a genome has provided a universal platform for targeted genome modification. ZFNs are artificial restriction endonucleases that comprise of an array of 3- to 6-C2H2-zinc finger DNA-binding domains fused with the dimeric cleavage domain of the type IIs endonuclease FokI. C2H2-zinc fingers are the most common, naturally occurring DNA-binding domain, and their specificity can be engineered to recognize a variety of DNA sequences providing a strategy for targeting the appended nuclease domain to desired sites in a genome. The utility of ZFNs for gene editing relies on their activity and precision in vivo both of which depend on the generation of ZFPs that bind desired target sites high specificity and affinity. Although various methods are available that allow construction of ZFPs with novel specificities, ZFNs assembled using existing approaches often display negligible in vivo activity, presumably resulting from ZFPs with either low affinity or suboptimal specificity. A root cause of this deficiency is the presence of interfering interactions at the finger-finger interface upon assembly of multiple fingers. In this study we have employed bacterial-one-hybrid (B1H)-based selections to identify two-finger zinc finger units (2F-modules) containing optimized interface residues that can be combined with published finger archives to rapidly yield ZFNs that can target more than 95% of the zebrafish and human protein-coding genes while maintaining a success rate higher than that of ZFNs constructed using available methods. In addition to genome engineering in model organisms, this advancement in ZFN design will aid in the development of ZFN-based therapeutics. In the process of creating this archive, we have undertaken a broader study of zinc finger specificity to better understand fundamental aspects of DNA recognition. In the process we have created the largest protein-DNA interaction dataset for zinc fingers to be described that will facilitate the development of better predictive models of recognition. Ultimately, these predictive models would enable the rational design of synthetic zinc finger proteins for targeted gene regulation or genomic modification, and the prediction of genomic binding sites for naturally occurring zinc finger proteins for the construction of more accurate gene regulatory networks.