Using trauma theory, I analyze the disjointed narrative structure of gothic works from 1764-1853 as symptomatic of the traumatic experience. Gothic novels contain multiple structural anomalies, including gaps in experience that indicate psychological wounding, use of the supernatural to violate rational thought, and the inability of witnesses to testify to the traumatic event. These structural abnormalities are the result of trauma that characters within these texts then seek to prevent or repair via detection.

Fluorescence-based assays have gained an ever-increasing popularity in life sciences. One of these rapidly emerging techniques is Protein Induced Fluorescence Enhancement (PIFE). Traditional explanations of PIFE focused exclusively on the role of the protein and largely neglected the role of the mediator DNA. In the same time, the existing models of PIFE were denying its exactly opposite effect. In the first part of the current dissertation we focus on a better understanding of PIFE, stimulated by the direct observation of its opposite effect, Induced Fluorescence Enhancement Quenching (PIFQ). This study offered us the leverage for obtaining on-demand fluorescence modulation in cyanine dyes. The following two chapters harvest this control over fluorescence modulation to generate two biotechnology applications: a sensitive potassium sensor with embedded fluorescent transducer, and a simple protocol for the fluorescent detection of His-tagged proteins. In the last part, a variety of fluorescence tools including Förster resonance energy transfer, fluorescence enhancement, and fluorescence quenching are employed for a much more complex task; to demystify the behavior of the human Maturation of Okazaki Fragments (MOF) machinery. First, we reconstituted the human MOF reaction and showed that it behaves considerably different than its well-established yeast homolog. Subsequently, our toolbox of fluorescence-based assays was used to pinpoint the kinetics and dynamics that lead to this unexpected MOF behavior.

Fragment-based virtual screening is an in-silico method that potentially identifies new startingpoints for drug molecules and provides an inexpensive and fast exploration of the relevantchemical space compared to its experimental counterpart. It focuses on docking small potentialbinding fragments to a binding pocket and is used to design improved binders by growing thefragments or joining fragments using suitable linkers. In this project, a fragment-based virtualscreening was evaluated by docking 21 fragments that are obtained from 4 different drugs. Here,the fragments were evaluated using SP score in place and SP and XP flexible docking methodsand were compared to the results of the two decoy fragment datasets. Three of the investigatedfragments are positioned at the top and docked with the correct poses and pockets when comparedto the corresponding substructure in the crystal structure and thus could be considered a successfulfragment starting points. Out of the two flexible docking methods used, the SP method providedadditional correct poses and pockets than XP in this limited dataset.

The thesis is an investigation of literary spaces and relationships.  How could the words from a novel translate into Architecture by using imagination and memories. Could the characters of imagination give a site or a space a certain atmosphere or create certain fragments of memories.

This thesis takes its starting point in four Stockholm locations; a self-service laundromat, a residential townhouse, a pedestrian tunnel and a public bath. They have emerged during different times but are brought together by their habitual and mundane character. They possess qualities that may not be seen at first glance, but becomes visible at a second or third viewing. The aim for this thesis has been to find what lies slightly out of sight – hidden yet visible. Through several steps of translations and fragmentation new scenarios have emerged.

<p> Lower Cretaceous Mannville Group sandstones of Alberta were deposited in non-marine and marginal environments. A shallow sea transgressed several times over the study area and left evidence of tidal action. The proportion of rock fragments increases from Lower Mannville to Upper Mannville sandstones due to the uplifting of strata to the west. </p> <p> Observed petrographic and SEM textures indicate that authigenic pyrite, quartz and calcite cements were precipitated in that order followed by the dissolution of carbonate material and feldspar grains with the simultaneous precipitation of kaolinite and quartz. The secondary (or dissolution) porosity is the result of an influx of acidic pore waters. This secondary porosity is best developed in the Ellerslie Sandstone because its remnant intergranular porosity and permeability are superior to the porosity and permeability in the overlying sandstones. The present degree of diagenesis in the sandstones is largely controlled by the permeability of the rock. </p> / Thesis / Bachelor of Science (BSc)

<p> Near Medicine Hat, Alberta, Pleistocene deposits are exposed on numerous bluffs along the South Saskatchewan River. The Quaternary beds are very fossiliferous, yielding a large number of mammal bones and teeth. The enamel (calcium hydroxyapatite) portion of teeth within the sediments, is used to date the deposits with the electron spin resonance (ESR) dating method. The ESR age is strongly dependent on the dose rate which in turn depends on the uranium accumulation in the tooth fragments. Two U uptake models are used based on an early and a continuous, linear accumulation of uranium.</p> <p> At young, well dated sites the ESR ages are in relatively good agreement with independent estimates. For slightly older samples (approx. 100 ka), the linear U uptake model comes closer to the estimated age at one site, but at another site, the early U uptake model agrees more favourably with the estimated age. Based on the ESR ages, several older sites can be assigned to interglacial stages 7, 9 and 13, however, these ages are much younger than those determined by faunal and stratigraphic correlation (>0.5 Ma). Samples with extremely high U contents in dentine and enamel gave unreliable ESR results. These samples may have experienced a late stage of U accumulation.</p> / Thesis / Master of Science (MSc)

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Several methods for the palaeobotanical analysis of inorganic rocks are developed and through the employment of these methods both microfossils and macrofossils have been recovered from certain Upper Ordovician Strata. These fragments are assigned to series, basing such designation upon their sources and individual morphological characteristics. The problematical identity and biological importance of these fragments is discussed, and their practical usage in problems of correlation is considered. / Thesis / Master of Science (MSc)