Master thesis is focused on proposal of improved manufacturing process of assembly named "sandwich" produced in Iveco bus company. There is solved new fixation of assembly bench, replacement of currently used glues and material of ledge as well. In the first part there is company introduction Iveco Bus. Second part consist of construction analysis and description of present manufacturing process. Further there is introduction, solving out of thesis topics and proposal of new manufacturing process is written. At the end of thesis are attached comparison between present and proposed manufacturing process.

There is an extended concern for how to quantify the fluxes of greenhouse gasses (GHG) and how they are related to climate change and land use. Efforts have already been done, mostly regarding carbon (C) compounds, but there is still much to be done especially to understand regulating factors and interactions with the Nitrogen cycle. NitroEurope is a large project for the integrated European research into the N cycle. This study shares its aim, to improve the understanding the physics involved in the fluxes of the reactive N (Nr). More specifically, the patterns of some physical and biological processes related to Nr have been studied for two wetlands, one in the proximity of Edinburgh and the other in the North of Finland.   A common model setup has been used for both of them, since they are both peat soils. The differences applied to achieve appropriate performances give insights of their nature. The presence of snow is a major factor that governs the behavior of the site in Finland. The scaling of existing models for the estimation of heat transfer in the soil of the Scottish site is also critical, such as its groundwater level. The sites represent different climatic conditions, but for CO2 fluxes they showed similar behavior and response to governing meteorological conditions. Using the GLUE method in combination with a process oriented ecosystem model, some further insights have been gained for the regulation and control of greenhouse gas emissions from the two sites.

How do additional data of the same and/or different type contribute to reducing model parameter and predictive uncertainties? This was the question addressed with two models – the HBV hydrological water balance model and the ICBM soil carbon balance model – that were used to investigate the usefulness of the Generalized Likelihood Uncertainty Estimation (GLUE) method for calibrations and uncertainty analyses.  The GLUE method is based on threshold screening of Monte Carlo simulations using so-called informal likelihood measures and subjective acceptance criterion. This method is highly appropriate for model calibrations when errors are dominated by epistemic rather than stochastic uncertainties.  The informative value of data for model calibrations was investigated with numerous calibrations aimed at conditioning posterior parameter distributions and boundaries on model predictions.  The key results demonstrated examples of: 1) redundant information in daily time series of hydrological data; 2) diminishing returns in the value of continued time series data collections of the same type; 3) the potential value of additional data of a different type; 4) a means to effectively incorporate fuzzy information in model calibrations; and 5) the robustness of estimated parameter uncertainty for portability of a soil carbon model between and tropical climate zones.  The key to obtaining these insights lied in the methods of uncertainty analysis used to produce them.  A paradigm for selecting between formal and informal likelihood measures in uncertainty analysis is presented and discussed for future use within a context of climate related environmental modeling. / QC 20110414

京都大学 / 0048 / 新制・課程博士 / 博士(理学) / 甲第18060号 / 理博第3938号 / 新制||理||1567(附属図書館) / 30918 / 京都大学大学院理学研究科物理学・宇宙物理学専攻 / (主査)教授 石田 憲二, 教授 前野 悦輝, 教授 松田 祐司 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

<p dir="ltr">The goal of this thesis is to design nature-inspired biomimetic materials that recapitulate essential features of tissues for biomedical applications including tissue modeling of drug transport and surgical adhesion.</p><p dir="ltr">The first part of this thesis utilizes collagen and glycosaminoglycans to mimic tissues for preclinical modeling of large-molecule drug transport. We first utilize hydrazone crosslinking chemistry with hyaluronic acid to form interpenetrating networks with collagen at different concentrations. The interpenetrating networks enabled a wide range of mechanical properties, including stiffness and swellability, and microstructures, such as pore morphology and size, that can better recapitulate diverse tissues. The mechanical and microstructural differences translated into differences in transport of the macromolecules of different sizes and charges from these matrices. Large macromolecules were impacted by mesh size, whereas small macromolecules were influenced primarily by electrostatic forces. The tunable properties demonstrated by the collagen and crosslinked hyaluronic acid hydrogels can be used to mimic different tissues for early-stage assays to understand drug transport and its relationship to matrix properties.</p><p dir="ltr">We then explore how the glycosaminoglycans hyaluronic acid, chondroitin sulfate, and heparin in collagen hydrogels influence drug transport via glycosaminoglycan-drug interactions and network development. Incorporating different types and concentrations of glycosaminoglycans led to glycosaminoglycan-collagen hydrogels with a range of collagen networks and negative charge densities to recapitulate different tissue compositions. Hyaluronic acid increased the overall viscosity of the hydrogel matrix, and chondroitin sulfate and heparin altered collagen fibrillogenesis. All three GAGs formed concentration-dependent polyelectrolyte complexes with positively charged macromolecules. Transport of positively charged macromolecules through collagen gels with chondroitin sulfate and high concentrations of heparin was inhibited due to complexation and charge effects. Conversely, collagen with low concentrations of heparin hastened the transport of macromolecules due to the limited collagen network resulting from fibrillogenesis inhibition. Overall, the addition of different GAGs into tissue models can better recapitulate native tissue to accurately predict therapeutics transport through a variety of tissues.</p><p>17</p><p dir="ltr">The second part of this thesis investigates the impact of pH and oxidation on an elastin- and mussel-inspired surgical sealant. We combined sodium periodate, an oxidizer, with an L-3,4-dihydroxyphenylalanine-modified elastin-like polypeptide to elucidate how the crosslinking mechanism and intermediate formation impacted adhesion, cure time, and stiffness. Formulations resisted burst pressures greater than physiological internal pressures. They did not swell and had stiffnesses similar to those of soft tissues, and their gelation times varied from seconds to hours. Small increases in the formulation pH led to the formation of α,β-dehydrodopamine intermediates which facilitated the development of multiple crosslinking networks. The mussel-inspired elastin-like adhesive can serve as a model of mussel proteins to further improve our understanding of mussel chemistry. This study exemplifies the importance of pH and oxidation on the performance of mussel-inspired adhesives in surgical sealing within physiological environments.</p><p dir="ltr">The final part of this thesis explores using biomimetic designs in an outreach activity aimed at engaging high school women in chemical engineering. The design and application of the activity led to increased interest in chemical engineering among the participants. There was greater alignment between students' aspirations and the field of chemical engineering, highlighting the potential for such outreach initiatives to inspire future generations of chemical engineers.</p>

京都大学 / 新制・論文博士 / 博士(医学) / 乙第13587号 / 論医博第2307号 / 新制||医||1070(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 安達 泰治, 教授 森本 尚樹, 教授 伊達 洋至 / 学位規則第4条第2項該当 / Doctor of Medical Science / Kyoto University / DFAM

名古屋大学博士学位論文 学位の種類 : 博士(医学)(論文) 学位授与年月日:平成3年2月1日 服部寿門氏の博士論文として提出された

I denna rapport utforskas möjligheterna med BIM-modeller som finns på dagens byggarbetsplatser.  Trots att BIM erbjuder effektivare lösningar inom områden som bland annat visualisering, samordning och kvalitetsarbete, är det få projekt som aktivt använder sig av BIM på byggarbetsplatsen. Den här rapporten syftar till att belysa potentialen med BIM under produktionsfasen samt analysera svårigheter som finns, då produktionspersonal vill använda sig av BIM-modellen. Det långsiktiga målet med rapporten är att främja framtida användning av BIM i produktionsfasen. I arbetet produktionsanpassas delar av BIM-modellen för Skanskas och Vasakronans samverkansprojekt Klara C till att den kan användas ifrån en handhållen enhet. Genom att visa praktiska tillämpningar, med verktyg och BIM-modeller som finns tillgängliga idag, synliggörs möjligheter med BIM i byggproduktionen. För att ha möjlighet att påverka kvalitet och format på den BIM-modell, vilken ändå uppförs vid projekteringen, är det av stor vikt att tidigt beakta vilka effekter ett aktivt användande av BIM har i produktionsfasen. Slutsatsen sammanfattas i ett antal frågeställningar som planerande personal bör ha i åtanke, detta för att främja en utvecklande och värdeskapande användning av BIM även ute på byggarbetsplatsen. Vilken nytta tillför modellen i det specifika projektet? Ex. Samordning, tidsplanering eller visualisering. Hur ska modellen användas av produktionspersonalen? Ex. Vilken enhet (Surfplatta/PC) Vilka krav ställs då på BIM-modellen? Ex. Detaljeringsnivå, parametrar. Vad krävs av arbets- och informationsflöden? Ex. Format på leverans av modell/modeller. Är tillämpningen anpassad till användaren? Ex. Behövs det ytterligare stöd i form av kortare utbildningar eller kurser? / This thesis explores the potential of the BIM-models available at today's construction sites. There are few projects that actively use BIM on the construction sites, despite the fact that BIM offers efficient solutions in areas such as visualization, coordination and quality control. This report aims to highlight the potential of BIM during the production phase and to analyze the difficulties that exist when production staff wants to use the BIM model. The long term goal of this report is to promote use of BIM in the production phase. In this report, parts of the BIM-model of Skanska and Vasakronan collaborative project Klara C, is adapted so that it can be used from a handheld device. Positive effects from active use of BIM-models are shown with the tools and BIM-models that are available today. In order to influence the quality and setup of the BIM-model, which is produced during the design stage, it is of great importance that all potential applications of the model is taken in to account. The conclusion is summarized in a number of questions that planning staff should keep in mind in order to promote value-adding use of BIM. What benefits does the model bring the specific project? E.g. Coordination, time planning or visualization. How will the model be used by the staff? E.g. What kind of unit? (E.g. Tablet or PC) What is required from the model? E.g. Level of detailing, parameters. What work- and information flows are required? E.g. How is the model/models updated and delivered. Is the application user friendly? E.g. Is additional, supporting education required?

The task of this diploma thesis was to investigate the influence of inorganic pigments on the identification of casein oil and animal glue tempera by mass spectrometry MALDI- -TOF MS (Matrix-Assisted Laser Desorption/Ionisation - Time of Flight Mass Spectrometry). First, it was necessary to prepare a set of model colour layers containing casein and animal glue tempera together with ten inorganic pigments. Consequently, the method of peptide mass fingerprinting was used to obtain mass spectra of the samples. Finally, it was possible to compare and determine the characteristic peptide fragments for both proteinaceous binders and find pigments that most affected the identification of casein and rabbit glue temperas. The obtained values of m/z casein oil tempera and animal glue tempera were used to improve and complete reference database of protein binders that is used to identify protein binders in works of art at the Institute of Chemical Technology in Prague.

Regulated secretion of hormones, digestive enzymes and other biologically active molecules requires formation of secretory granules. However, the molecular machinery required for secretory granule biogenesis is incompletely understood. I used powerful genetic approaches available in the fruit fly Drosophila melanogaster to investigate the factors required for biogenesis of mucin-containing ‘glue granules,’ which form within epithelial cells of the third-instar larval salivary gland. I discovered that clathrin and the clathrin adaptor protein complex (AP-1), as well the enzyme type II phosphatidylinositol 4-kinase (PI4KII), are indispensable for glue granule biogenesis. Clathrin and AP-1 are necessary for maturation of exocrine, endocrine and neuroendocrine secretory granules in mammalian cells. I found that Drosophila clathrin and AP-1 colocalize at the TGN and that clathrin recruitment requires AP-1. I further showed that clathrin and AP-1 colocalize with secretory cargo at the TGN and on glue granules. Finally, I demonstrated that loss of clathrin or AP-1 leads to a profound block in secretory granule biogenesis. These findings establish a novel role for AP-1/clathrin-dependent trafficking in the formation of mucin-containing secretory granules. Type II phosphatidylinositol 4-kinase (PI4KII) generates the membrane lipid phosphatidylinositol 4-phosphate (PI4P) at the trans-Golgi network and is required to recruit cargo to endosomes in mammalian cells. I generated null mutations in the sole Drosophila PI4KII and demonstrated a role for PI4KII in both glue granule and pigment granule biogenesis. PI4KII mutant salivary gland cells exhibit small glue granules and mislocalize glue protein to abnormally large late endosomes. Additionally, PI4KII mutants exhibit altered distribution of the granule specific SNARE, SNAP-24. These data point to a crucial role for PI4KII in sorting of regulated secretory products during granule biogenesis. Together, my results indicate that the larval salivary gland is a valuable system for investigating molecular mechanisms involved in secretory granule biogenesis, and provide a framework for future studies using this system.