Nagrinėjama besiūlio įrišimo (klijavimo) kokybė. Aprašomas besiūlio įrišimo specifiškumas, klijavimo mašinos parametrai, darantys įtaką suklijavimo kokybei, ypač pirmam ir paskutiniam puslapiui. Pateikiami rezultatai klijuojant skirtingais suklijavimo parametrais. Rezultatai apdorojami eksperimentų planavimo metodu. Parodytas principas ieškant rezultatų eksperimento planavimo metodu. Darbą sudaro 8 dalys: įvadas, literatūros apžvalga, tyrimų metodika, rezultatai ir jų aptarimas, išvados ir rekomendacijos, santrumpos, literatūros sąrašas, priedai. Darbo apimtis – 57 p. teksto be priedų, 9 lentelės, 25 paveikslai, 10 bibliografiniai šaltiniai. / It is presented the research of glue binding machine. It is discribed the specificity of glue binding, the parameters, which are the most important for glue binding quality, especially for binding first and last pages. There are given results of binding, when different parameters are chosen. It is also shown the principle of counting by method of planning experiment. Thesis consist of: 57 p. text without appendixes, 9 tables,25 pictures, 10 bibliographical entries.

Regulated secretion of hormones, digestive enzymes and other biologically active molecules requires formation of secretory granules. However, the molecular machinery required for secretory granule biogenesis is incompletely understood. I used powerful genetic approaches available in the fruit fly Drosophila melanogaster to investigate the factors required for biogenesis of mucin-containing ‘glue granules,’ which form within epithelial cells of the third-instar larval salivary gland. I discovered that clathrin and the clathrin adaptor protein complex (AP-1), as well the enzyme type II phosphatidylinositol 4-kinase (PI4KII), are indispensable for glue granule biogenesis. Clathrin and AP-1 are necessary for maturation of exocrine, endocrine and neuroendocrine secretory granules in mammalian cells. I found that Drosophila clathrin and AP-1 colocalize at the TGN and that clathrin recruitment requires AP-1. I further showed that clathrin and AP-1 colocalize with secretory cargo at the TGN and on glue granules. Finally, I demonstrated that loss of clathrin or AP-1 leads to a profound block in secretory granule biogenesis. These findings establish a novel role for AP-1/clathrin-dependent trafficking in the formation of mucin-containing secretory granules. Type II phosphatidylinositol 4-kinase (PI4KII) generates the membrane lipid phosphatidylinositol 4-phosphate (PI4P) at the trans-Golgi network and is required to recruit cargo to endosomes in mammalian cells. I generated null mutations in the sole Drosophila PI4KII and demonstrated a role for PI4KII in both glue granule and pigment granule biogenesis. PI4KII mutant salivary gland cells exhibit small glue granules and mislocalize glue protein to abnormally large late endosomes. Additionally, PI4KII mutants exhibit altered distribution of the granule specific SNARE, SNAP-24. These data point to a crucial role for PI4KII in sorting of regulated secretory products during granule biogenesis. Together, my results indicate that the larval salivary gland is a valuable system for investigating molecular mechanisms involved in secretory granule biogenesis, and provide a framework for future studies using this system.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The present research had the objective to evaluate the use of an n-butil cyanoacrylate in the fixation and maintenance of corneal autografts and aloimplants in the penetrating keratoplasty. There were used eight adult rabbits divided in two groups: the group I adhesive (GI 4 animals) and group II suture (GII 4 animals). In the GI, performed in the left eye, a penetrating keratoplasty with 5 mm in diameter received a 6 mm in diameter alogenous corneal button preserved in glycerin 98%, fixed with 4 simple interrupted stitches and with cyanoacrylate adhesive. In the right eye, the same procedure were done, but with a 4 mm in diameter defect with corneal left button that was removed (5 mm). In the GII, a similar procedure were done, but the corneal buttons were fixed with 8 simple interrupted stitches with mononylon 10-0. The pos-operatory (PO) ophthalmic evaluation were used the computer videoceratoscopy, ultrasound pachimetry and scanning eletronic microscopy. It was verified the integration of both transplants in both grafts. A bigger astigmatism grade were observed in the autotransplants from GI, and also with a lesser endothelial density. The pachimetry, were verified the decrease of the corneal thickness as the time elapsed. In all animals it was noted a complete endothelial progressive over the grafst and implants. It was concluded that the cyanoacrylate adhesive can be used as a fixative method in corneal autotransplants and also in corneal alografts in rabbits. / A procura de alternativas para a fixação dos enxertos utilizados em defeitos corneais é realizada de forma incessante. Os cianoacrilatos são monômeros líquidos que em contato com a umidade polimerizam-se e solidificam-se rapidamente ocasionando a adesão nos tecidos. São utilizados em diversas situações clínicas na oftalmologia humana e veterinária. O presente trabalho teve como objetivo, avaliar o uso de um adesivo a base de n-butil cianoacrilato na fixação e manutenção de botões corneais autógenos e alógenos na ceratoplastia penetrante. Para tanto foram usados 16 coelhos divididos em dois grupos: O grupo I adesivo (GI 8 animais) e grupo II sutura (GII 8 animais). No GI, foi realizada, no olho esquerdo, uma ceratoplastia penetrante de 5 mm utilizando-se córnea alógena conservada em glicerina de 6 mm de diâmetro e fixando-a com 4 pontos isolados simples e adesivo de cianoacrilato sobre as bordas. No olho direito também foi realizada uma ceratoplastia penetrante, porém de 4 mm de diâmetro utilizando-se o botão que foi removido da córnea esquerda (5 mm) com o mesmo método de fixação. No GII procedimentos semelhantes fora realizados, porém, a fixação dos botões constou de 8 pontos isolados simples. Avaliações oftálmicas pós-operatórias (PO) foram realizadas. Aos 0 (antes dos procedimentos) 60 e 90 dias PO foram realizadas a videoceratoscopia computadorizada e a paquimetria ultra-sônica. Ao final do período de avaliação, as córneas foram avaliadas à microscopia eletrônica por varredura. Foi verificada a integração de ambos os transplantes em ambos os grupos. Houve um maior grau de astigmatismo visto nos transplantes autógenos do GI e, neste grupo a densidade endotelial foi menor. À paquimetria, foi verificada a diminuição da espessura conforme aumentavam os tempos. Em todos os animais houve a completa progressão do endotélio corneal sobre o aloimplante. Conclui-se que o adesivo de cianoacrilato pode ser usado como método de fixação de autotransplantes bem como alotransplantes de córnea em coelhos.

Submitted by JOÃO CARLOS BIAZZON null (carlosbiazzon@bol.com.br) on 2016-12-19T16:22:00Z No. of bitstreams: 1 Dissertação João Carlos Biazzon.pdf: 9362825 bytes, checksum: 73ab148e606959b23c89521eeb67a86e (MD5) / Approved for entry into archive by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-12-21T10:59:59Z (GMT) No. of bitstreams: 1 bizzon_jc_me_bauru.pdf: 9362825 bytes, checksum: 73ab148e606959b23c89521eeb67a86e (MD5) / Made available in DSpace on 2016-12-21T10:59:59Z (GMT). No. of bitstreams: 1 bizzon_jc_me_bauru.pdf: 9362825 bytes, checksum: 73ab148e606959b23c89521eeb67a86e (MD5) Previous issue date: 2016-12-07 / O presente trabalho teve como objetivo, verificar o desempenho de juntas coladas em função da qualidade das superfícies da madeira usinadas em operações de fresamento cilíndrico tangencial para diferentes velocidades de avanço, empregando-se quatro tipos de adesivos à base de Poliacetato de Vinila (PVAc) denominados como C1, C2, C3 e C4 e madeira da espécie Eucalyptus saligna. Para tanto, foram confeccionadas amostras para ensaios normatizados pela Norma ABNT - NBR 7190:1997, submetidas a testes de resistência à ruptura na linha de cola por esforços de cisalhamento. Na união das peças de madeira para confecção das amostras, essas foram coladas em planos aleatórios em relação aos anéis de crescimento da madeira. As amostras foram usinadas empregando-se as velocidades de avanço V1= 6,0; V2 = 11,0; V3 = 16,0 e V4 = 21,0 m.min-1, correspondendo, respectivamente aos avanços por dente “fz”: 0,25 mm; 0,45 mm; 0,66 mm e 0,87 mm. Pôde-se verificar um melhor desempenho para uso do adesivo “C4” e melhor superfície de adesão para a velocidade de avanço V1 = 6,0 m.min-1. Dos resultados podem-se destacar o melhor desempenho empregando-se o adesivo C4, e o pior desempenho o adesivo C2 para todas as velocidades de avanço testadas. / The present work aimed at verifying the performance of bonded joints in function to the quality to the wood surfaces machined tangential cylindrical milling operations to different advance speeds, using four types of adhesives based on vinyl polyacetate (PVAc) denominated as C1, C2, C3 and C4 and wood species Eucalyptus saligna. For this, samples were prepared for standardized tests by ABNT - NBR 7190:1997, subjected to tensile strength tests on the glue line shear efforts. In the union of pieces of wood for making the samples, these were glued on random plans in relation to the wood growth rings. The samples machined using the advance speed V1 = 6.0; V2 = 11.0; V3 = 16.0 and V4 = 21.0 m. min-1, corresponding respectively to advances by tooth "fz": 0.25 mm; 0.45 mm; 0.66 mm and 0.87 mm. It was verified better performance for adhesive use "C4" and better adhesion surface for the advance speed V1 6.0 m. min-1. From the results, it can be highlighted the best performance using the C4 adhesive, and the worst performance C2 adhesive for all tested advance speeds.

Submitted by Jaqueline Silva (jtas29@gmail.com) on 2016-09-13T17:34:46Z No. of bitstreams: 5 Dissertação - Helder Amorim Silva Borges de Deus - 2016 (1).pdf: 16375896 bytes, checksum: fa3e3bb95cf6f30a32a1dd8d1a529846 (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (2).pdf: 15731290 bytes, checksum: 50295692526e16122da9589638dcfadd (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (3).pdf: 10294020 bytes, checksum: 7e3b48ef326b52364ef11302dc09586f (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (4).pdf: 18265933 bytes, checksum: 59523587b394d1e88ff8655f19e1905f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Jaqueline Silva (jtas29@gmail.com) on 2016-09-13T17:35:05Z (GMT) No. of bitstreams: 5 Dissertação - Helder Amorim Silva Borges de Deus - 2016 (1).pdf: 16375896 bytes, checksum: fa3e3bb95cf6f30a32a1dd8d1a529846 (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (2).pdf: 15731290 bytes, checksum: 50295692526e16122da9589638dcfadd (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (3).pdf: 10294020 bytes, checksum: 7e3b48ef326b52364ef11302dc09586f (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (4).pdf: 18265933 bytes, checksum: 59523587b394d1e88ff8655f19e1905f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-09-13T17:35:05Z (GMT). No. of bitstreams: 5 Dissertação - Helder Amorim Silva Borges de Deus - 2016 (1).pdf: 16375896 bytes, checksum: fa3e3bb95cf6f30a32a1dd8d1a529846 (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (2).pdf: 15731290 bytes, checksum: 50295692526e16122da9589638dcfadd (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (3).pdf: 10294020 bytes, checksum: 7e3b48ef326b52364ef11302dc09586f (MD5) Dissertação - Helder Amorim Silva Borges de Deus - 2016 (4).pdf: 18265933 bytes, checksum: 59523587b394d1e88ff8655f19e1905f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-04-07 / The research entitled Image engraved: from cardboard matrixes and glue vinyl to chalcography impressions proposes a study in two steps. In the first instance, it aims the production of matrixes and engravings, exploring the combination, materiality and the versatility of two materials which are considered alternative when compared to the traditional use of wood for woodcuts, of metal plates for chalcography, of linoleum for linocuts and stones for lithographs: the cardboard and glue vinyl. Then, these matrixes, which emphasized the chalcography’s processes, were presented to one group of visually impaired sharing narratives constructed by dialogues between our interpretations. / A pesquisa intitulada Imagem gravada: das matrizes de papelão e cola vinil às impressões em encavo propõe um estudo em duas etapas. Em um primeiro momento, dedica-se à produção de matrizes e gravuras explorando a combinação, a materialidade e a versatilidade de dois materiais considerados alternativos, quando comparados ao tradicional uso da madeira nas xilogravuras, das chapas de metal nas calcografias, do linóleo nas linóleogravuras e da pedra calcária nas litografias: o papelão e a cola vinil. Em seguida, essas matrizes, que deram ênfase aos processos de impressão em encavo, foram apresentadas a um grupo de deficientes visuais compartilhando narrativas construídas pelos diálogos entre as nossas interpretações.

Les agents d'embolisation liquides utilisés dans le traitement endovasculaire ont de nombreuses limitations. Des polymères, tels que l’Onyx et les cyanoacrylates sont disponibles. L’alcool est un agent puissant, mais non radio-opaque. Les cyanoacrylates entrainent une réaction inflammatoire significative. Leur polymérisation rapide est responsable d’un comportement mal prévisible.Il existe une possibilité de traitement incomplet.L’Onyx est efficace pour le traitement des MAV.Une pénétration plus distale est obtenue avec l’alcool, mais associée à un risque de migration systémique. Nous avons évalué les propriétés d'un nouvel agent embolique (Alconyx) composé d'un mélange d'alcool et d'Onyx. Cet agent devrait cumuler les avantages respectifs de l'alcool et de l'onyx avec une visualisation adéquate sous fluoroscopie, une meilleure pénétration distale que l'Onyx seul et moins de toxicité systémique liée à la réduction de quantité d’alcool injecté. Divers mélanges ont été testés avec différentes concentrations d'Onyx 18 et d’alcool absolu. Alconyx 25 (75% Onyx 18; 25% d'éthanol) est la formulation la plus prometteuse. Nous avons démontré sa facilité d'injection in vivo, sa nature cohésive sans fragmentation ainsi que sa bonne visualisation sous fluoroscopie. En raison de sa moindre viscosité démontrée in vitro, Alconyx a été capable de pénétrer profondément dans le lit artériel.. L'occlusion proximale par Alconyx 25 devrait permettre d'améliorer le contact entre l'éthanol et la paroi vasculaire et donc augmenter son pouvoir sclérosant et limiter son passage systémique. Les propriétés occlusives d’Alconyx 25 sont similaires à celle de l’Onyx 18 sous haute pression in vitro. / Commercially available liquid embolization agents used in endovascular treatment have many limitations. Polymeric agents as Onyx and cyanoacrylate are available. Ethanol also is a potent sclero-embolic agent. Cyanoacrylates are effective liquid embolic agents, however, their rapid polymerization makes their behaviour unpredictable with possibility of incomplete treatment. These properties render their use challenging.Onyx is easy to use. However, in very small arterial niduses, Onyx, is unable to penetrate deeply. Deep penetration is obtained with ethanol, associated with risk of systemic migration.Poor visualization of ethanol under fluoroscopy is major drawback. Mixing Onyx with ethanol had never been described in the literature till now. In this work, various mixtures have been tested with different concentrations of Onyx 18 and absolute ethanol. Alconyx 25 (75% Onyx 18; 25% ethanol) seems to be a promising product. We proved its ease of injection in vivo and in vitro, its cohesive nature showing no fragmentation or interruption of the injected column as well as its good visualization under fluoroscopy. It was able to penetrate deeply in the arterial bed. The occlusive properties of Alconyx 25 were rated as good as Onyx 18 under high pressure in vitro. Further investigation is needed to better understand the behavior of ethanol in the suspension and its effect on tissues compared to Onyx diluted simply with an equivalent amount of DMSO. Studies on other commercially available concentrations of Onyx would certainly be interesting.

The aim of this diploma thesis is to prepare the project documentation for operational building design documentation of a pension with restauration. I it an object with three above-ground floors and one underground floor. The structural construction system of the building is mixed. The technical report and the drawing documentation is a part of the thesis.

The main object of this master´s thesis is to increase productivity glueing and lowering the weight of the bus roof while maintaining its toughness. The first part describes the present glueing technologies. Subsequently, there is adduced theoretically various hypothesis about the glue seam. The proposed solution is to use the glue containing accelerators. Two glues from the companies DINITROL and SIKA are suggested and compared. The following chapter details the suggested technology and describes its possibilities. The final part presents the project of ultralight roof made from sandwich panels.

Då Tarfalahallens tak kollapsade i Mars 2020 kallades den SvenskaHaverikommissionen in för att utreda bakomliggande orsaker tillraset. En av de primära orsakerna bakom raset var en vippad balk iden bärande takkonstruktionen. Takkonstruktionen utgjordes avunderspända takstolar, en konstruktion som på grund avHaverikommissionens utredning och påföljande anmälan tilltillsynsmyndigheten och Boverket skulle komma att bli ifrågasattgällande dess säkerhet. Byggnadsnämnderna i Sveriges kommunerfick 2020/2021 i uppdrag att undersöka om byggnader medunderspända limträtakstolar finns, byggs eller projekteras inomderas verksamhetsområde. Om så var fallet, raskt bedömastabiliteten i konstruktionerna, vidta nödvändiga åtgärder därinstabilitet upptäcktes samt rapportera alla fynd tillbaka tillBoverket.Då ledande svensk litteratur inom limträkonstruktion ej behandlarunderspända konstruktioner i någon större utsträckning fanns ettbehov av att se på vilka mekanismer som föranlederstabilitet/instabilitet för denna konstruktionstyp. Dettaexamensarbete ämnar försöka besvara dessa frågor och har genomen litteraturstudie sammanställt parametrar för stabilitet för dennakonstruktionstyp. Checklistor för initial stabilitetskontroll utifrånrelationshandlingar och inmätningsdata har tagits fram. Eninmätningsplan har skapats för att underlätta datainsamlingen förden initiala bedömningen. Ett förslag på ett övervakningssystemför konstruktionstypen har tagits fram och en bedömning avbefintliga takstolar har gjorts med hjälp av de checklistor somskapats under arbetets gång. Slutligen verifierades stabiliteten i detakstolar som kontrollerades genom en bärverksanalys enligtEurokod 5 och EKS 11 / In march of 2020 the roof of Tarfalahallen in Kiruna, Sweden,collapsed and the Swedish accident and incident investigationcommittee was called on site to investigate the causes for thestructural collapse. One of the primary reasons for the incident waslateral instability in one of the beams. The loadbearing constructionconsisted of underspanned beams. A construction type that cameinto question regarding its safety and practicality due to theinvestigations report and the following notification to thesupervisory authority. All Swedish municipalities were asked toinvestigate whether there were any buildings with thisconstruction type within their governing areas. If there were anyplans for constructing buildings with these roof systems or if anyunderspanned systems were in construction within their governingareas. If so, they were to investigate the stability of saidconstructions and take necessary precautions if instability wasfound and report back to the supervisory authority. Sinceinformation on underspanned, glue laminated timber structures,are scarce in leading Swedish literature there was a nedd to look atthe mechanisms behind what causes stability and instability withinthis construction type. The aim of this bachelor thesis is to try andanswer the questions regarding mechanisms behind stability andinstability for underspanned systems of glue laminated timber.This work also tries to answer the question regarding theconstruction-types safety and practicality. A literature study hasbeen performed to compile parameters regarding stability forunderspanned beams of glue laminated timber. Checklists havebeen produced to aid in the initial evaluation of stability. A planfor how to gather physical data through surveying has beenproduced for the initial evaluation. Lastly, an initial evaluation hasbeen performed on a set of underspanned beams with thechecklists produced in this thesis, a glue laminated timberstructure in Skellefteå, Sweden was evaluated. A loadbearinganalysis has been performed on the same set of beams inaccordance with Eurocode 5 and EKS 11 to verify the stability ofsaid beams. The indicators of stability from the initial evaluationwere confirmed by the loadbearing analysis. / <p>2022-06.21</p>

To propose a methodology for the uncertainty estimation in water quality modeling as related to TMDL development, four Monte Carlo (MC) based techniques—single-phase MC, two-phase MC, Generalized Likelihood Uncertainty Estimation (GLUE), and Markov Chain Monte Carlo (MCMC) —were applied to a Hydrological Simulation Program–FORTRAN (HSPF) model developed for the Mossy Creek bacterial TMDL in Virginia. Predictive uncertainty in percent violations of instantaneous fecal coliform concentration criteria for the prediction period under two TMDL pollutant allocation scenarios was estimated. The average percent violations of the applicable water quality criteria were less than 2% for all the evaluated techniques. Single-phase MC reported greater uncertainty in percent violations than the two-phase MC for one of the allocation scenarios. With the two-phase MC, it is computationally expensive to sample the complete parameter space, and with increased simulations, the estimates of single and two-phase MC may be similar. Two-phase MC reported significantly greater effect of knowledge uncertainty than stochastic variability on uncertainty estimates. Single and two-phase MC require manual model calibration as opposed to GLUE and MCMC that provide a framework to obtain posterior or calibrated parameter distributions based on a comparison between observed and simulated data and prior parameter distributions. Uncertainty estimates using GLUE and MCMC were similar when GLUE was applied following the log-transformation of observed and simulated FC concentrations. GLUE provides flexibility in selecting any model goodness of fit criteria for calculating the likelihood function and does not make any assumption about the distribution of residuals, but this flexibility is also a controversial aspect of GLUE. MCMC has a robust formulation that utilizes a statistical likelihood function, and requires normal distribution of model errors. However, MCMC is computationally expensive to apply in a watershed modeling application compared to GLUE. Overall, GLUE is the preferred approach among all the evaluated uncertainty estimation techniques, for the application of watershed modeling as related to bacterial TMDL development. However, the application of GLUE in watershed-scale water quality modeling requires further research to evaluate the effect of different likelihood functions, and different parameter set acceptance/rejection criteria. / Ph. D.